MD14G1240500.v1.1
MYB Family

Transcription repressor KAN1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0004135
Physical Location & Seq
Forward (+)
31941870 .. 31947077
5208 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1240500.v1.1.491

Sequence Viewer

Length: 1257 bp
ATGCTTCACGAAGAAGGGCTTTCATCAGAAGCAGCTGCAGGCGGCAGAAGCTGCTTATCTTTGAATCCGATTCCTGATCTTTCACTTCACATAAGCCCTCCAGACACAAACACAAATAGTGCTCCATCCTCCATTTGCACCGACCGAGCAACACCCGATCACGACTCCGCCAGCTGCTTCAACATATGGCGAAGAGACACCGACGAGGAAGATACCAACGGCGGCGCCCTAACCAACAAATCCCACAGCGACAGCTGTATTAGGGCCAGCTACACCAGTTCTTCCCCAGTAGCCGCTGATGCCGACACTGAGCTCTCCCTCGCCAATCCCTCGGCCTCAGAGGCCGAGAGCGCATGGAAGAAGAACTATTTCGGCGGCGGAGAAGTTTACCATGACGAAGGGAAGAATACTAGTATTAGTGCAAGTAGTGGCAATAAGATTCCGTTGCTGGAGAGACTCAACCCCATCAAAGGGATTCCTGTTTACGGTACTAGTAGTACTAATTGCTCGTTTCCTCCTACGTTTTCGCCTAATCCTGTGTACCAAACGGCGCCGTATCTGTCTAATTCCTTCAGACCGGCAGCACCTGCTGGGAATTGTAGTGGCATGGGCATGGGAATTGGAATAGGATTGGGAGGGTTTAACGGGATAACGATGGACAGTTTAAGCCTAGGAGTAGGAGGAGTGCAGCCACAACAGCCGCAGCATCAACGGCAACACTATTTTCCGTCCTATAATTTGAATCCTCATTACCCTCAGCAGCATCATTGTGTAGGGGGAGGTGGGAATTACAACGCCGACTTGTCGTCCAATGGTTTCATCATGAGATCTAGATTTACGCCTCCAAAGCTCATAGGGCAGAATATCAACAAGAGGAACATGAGAGCTCCCAGGATGCGATGGACTACTTCTCTTCACGCCCGTTTTGTTCATGCTGTCGAGCTCCTTGGTGGGGCTACTCCAAAGTCCGTGTTGGAGCTCATGGATGTGAAGGATCTCACACTTGCTCATGTTAAAAGCCATTTGCAGATGTATAGAACCGTTAAGAACACTGACAAACCTCTAGCTTCCTCTGATGGATCTGGGGATGACGATCTTTTGTCTACAACAAGCCATCATCATAATTCAAATTCCAATGGTTTACTTAACCAAAGAGGAGCCTCAAATGCAACTTTAAACTTAGAGCACGATCATATGGAACACCACCCTTCTAACAATCTATGGGGCAACTCCTCCAGGTATGATCTTCATTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

419

Amino Acids

45.03

Weight (kDa)

6.32

Isoelectric Point (pI)

43.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 298 - 346 8.6e-07 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 595
Acc36I ACCTGC 1 cut(s) 595
AccB1I GGYRCC 2 cut(s) 224, 550
AccI GTMKAC 1 cut(s) 1103
AciI CCGC 7 cut(s) 42, 168, 222, 294, 375, 378, 701
AclWI GGATC 2 cut(s) 1002, 1087
AcsI RAATTY 1 cut(s) 1129
AcuI CTGAAG 1 cut(s) 556
AcyI GRCGYC 2 cut(s) 225, 551
AfaI GTAC 3 cut(s) 490, 499, 542
AfiI CCNNNNNNNGG 4 cut(s) 470, 471, 485, 952
AgsI TTSAA 4 cut(s) 64, 181, 742, 1128
AhdI GACNNNNNGTC 1 cut(s) 805
AhlI ACTAGT 2 cut(s) 410, 491
AjnI CCWGG 2 cut(s) 890, 1235
Alw21I GWGCWC 6 cut(s) 124, 315, 889, 945, 981, 1188
Alw26I GTCTC 2 cut(s) 189, 448
AlwI GGATC 2 cut(s) 1002, 1087
AlwNI CAGNNNCTG 2 cut(s) 51, 587
AoxI GGCC 3 cut(s) 264, 333, 342
ApeKI GCWGC 8 cut(s) 32, 35, 51, 174, 581, 688, 703, 760
ApoI RAATTY 1 cut(s) 1129
Asp700I GAANNNNTTC 1 cut(s) 368
AspA2I CCTAGG 1 cut(s) 670
AspLEI GCGC 3 cut(s) 227, 353, 553
AspS9I GGNCC 1 cut(s) 264
AvrII CCTAGG 1 cut(s) 670
BanI GGYRCC 2 cut(s) 224, 550
BanII GRGCYC 4 cut(s) 315, 889, 945, 981
Bbv12I GWGCWC 6 cut(s) 124, 315, 889, 945, 981, 1188
BbvCI CCTCAGC 1 cut(s) 756
BbvI GCAGC 8 cut(s) 22, 38, 44, 161, 593, 700, 715, 772
BccI CCATC 6 cut(s) 133, 473, 649, 894, 1070, 1122
BceAI ACGGC 4 cut(s) 235, 538, 564, 728
BciT130I CCWGG 2 cut(s) 892, 1237
BcoDI GTCTC 2 cut(s) 189, 448
BcuI ACTAGT 2 cut(s) 410, 491
BfaI CTAG 5 cut(s) 411, 492, 671, 831, 1064
BfmI CTRYAG 1 cut(s) 36
BfoI RGCGCY 2 cut(s) 228, 554
BfuAI ACCTGC 1 cut(s) 595
BglI GCCNNNNNGGC 1 cut(s) 341
BglII AGATCT 1 cut(s) 827
BlnI CCTAGG 1 cut(s) 670
BmcAI AGTACT 1 cut(s) 499
Bme1390I CCNGG 2 cut(s) 892, 1237
BmeRI GACNNNNNGTC 1 cut(s) 805
BmgT120I GGNCC 1 cut(s) 264
BmiI GGNNCC 3 cut(s) 226, 552, 1159
BmrFI CCNGG 2 cut(s) 892, 1237
BmrI ACTGGG 1 cut(s) 281
BmsI GCATC 4 cut(s) 289, 715, 772, 885
BmuI ACTGGG 1 cut(s) 281
BpmI CTGGAG 3 cut(s) 84, 470, 1219
Bpu10I CCTNAGC 1 cut(s) 756
BsaBI GATNNNNATC 1 cut(s) 1092
BsaHI GRCGYC 2 cut(s) 225, 551
BsaJI CCNNGG 4 cut(s) 330, 670, 890, 946
Bsc4I CCNNNNNNNGG 4 cut(s) 470, 471, 485, 952
Bse118I RCCGGY 1 cut(s) 577
Bse1I ACTGG 2 cut(s) 276, 287
Bse8I GATNNNNATC 1 cut(s) 1092
BseBI CCWGG 2 cut(s) 892, 1237
BseDI CCNNGG 4 cut(s) 330, 670, 890, 946
BseGI GGATG 4 cut(s) 125, 900, 991, 1093
BseJI GATNNNNATC 1 cut(s) 1092
BseLI CCNNNNNNNGG 4 cut(s) 470, 471, 485, 952
BseMII CTCAG 3 cut(s) 300, 351, 770
BseNI ACTGG 2 cut(s) 276, 287
BseRI GAGGAG 3 cut(s) 696, 1170, 1222
BseXI GCAGC 8 cut(s) 22, 38, 44, 161, 593, 700, 715, 772
BseYI CCCAGC 1 cut(s) 590
BsgI GTGCAG 1 cut(s) 707
Bsh1285I CGRYCG 1 cut(s) 145
BshFI GGCC 3 cut(s) 266, 335, 344
BshNI GGYRCC 2 cut(s) 224, 550
BsiEI CGRYCG 1 cut(s) 145
BsiHKAI GWGCWC 6 cut(s) 124, 315, 889, 945, 981, 1188
BsiSI CCGG 1 cut(s) 578
BslI CCNNNNNNNGG 4 cut(s) 470, 471, 485, 952
BsmAI GTCTC 2 cut(s) 189, 448
BsnI GGCC 3 cut(s) 266, 335, 344
Bsp1286I GDGCHC 6 cut(s) 124, 315, 889, 945, 981, 1188
Bsp143I GATC 8 cut(s) 76, 157, 827, 994, 1079, 1093, 1189, 1243
BspACI CCGC 7 cut(s) 42, 168, 222, 294, 375, 378, 701
BspANI GGCC 3 cut(s) 266, 335, 344
BspCNI CTCAG 3 cut(s) 301, 350, 769
BspHI TCATGA 1 cut(s) 822
BspLI GGNNCC 3 cut(s) 226, 552, 1159
BspMAI CTGCAG 1 cut(s) 40
BspMI ACCTGC 1 cut(s) 595
BspPI GGATC 2 cut(s) 1002, 1087
BspT107I GGYRCC 2 cut(s) 224, 550
BsrFI RCCGGY 1 cut(s) 577
BsrI ACTGG 2 cut(s) 276, 287
BssAI RCCGGY 1 cut(s) 577
BssECI CCNNGG 4 cut(s) 330, 670, 890, 946
BssMI GATC 8 cut(s) 76, 157, 827, 994, 1079, 1093, 1189, 1243
BssNI GRCGYC 2 cut(s) 225, 551
BssT1I CCWWGG 2 cut(s) 670, 946
Bst2UI CCWGG 2 cut(s) 892, 1237
Bst4CI ACNGT 3 cut(s) 488, 662, 1042
Bst6I CTCTTC 2 cut(s) 187, 918
BstACI GRCGYC 2 cut(s) 225, 551
BstAPI GCANNNNNTGC 2 cut(s) 51, 587
BstC8I GCNNGC 3 cut(s) 40, 172, 268
BstDEI CTNAG 4 cut(s) 309, 337, 756, 1180
BstF5I GGATG 4 cut(s) 125, 900, 991, 1093
BstH2I RGCGCY 2 cut(s) 228, 554
BstHHI GCGC 3 cut(s) 227, 353, 553
BstKTI GATC 8 cut(s) 79, 160, 830, 997, 1082, 1096, 1192, 1246
BstMAI GTCTC 2 cut(s) 189, 448
BstMBI GATC 8 cut(s) 76, 157, 827, 994, 1079, 1093, 1189, 1243
BstMCI CGRYCG 1 cut(s) 145
BstNI CCWGG 2 cut(s) 892, 1237
BstSCI CCNGG 2 cut(s) 890, 1235
BstSFI CTRYAG 1 cut(s) 36
BstV1I GCAGC 8 cut(s) 22, 38, 44, 161, 593, 700, 715, 772
BstX2I RGATCY 3 cut(s) 827, 994, 1079
BstYI RGATCY 3 cut(s) 827, 994, 1079
BsuRI GGCC 3 cut(s) 266, 335, 344
BtgZI GCGATG 1 cut(s) 913
BtsCI GGATG 4 cut(s) 125, 900, 991, 1093
BtsIMutI CAGTG 2 cut(s) 306, 1050
BveI ACCTGC 1 cut(s) 595
Cac8I GCNNGC 3 cut(s) 40, 172, 268
CaiI CAGNNNCTG 2 cut(s) 51, 587
CciI TCATGA 1 cut(s) 822
CfoI GCGC 3 cut(s) 227, 353, 553
Cfr10I RCCGGY 1 cut(s) 577
Cfr13I GGNCC 1 cut(s) 264
Csp6I GTAC 3 cut(s) 489, 498, 541
CviAII CATG 9 cut(s) 354, 392, 607, 613, 823, 880, 932, 982, 1010
CviQI GTAC 3 cut(s) 489, 498, 541
DdeI CTNAG 4 cut(s) 309, 337, 756, 1180
DinI GGCGCC 2 cut(s) 226, 552
DpnI GATC 8 cut(s) 78, 159, 829, 996, 1081, 1095, 1191, 1245
DpnII GATC 8 cut(s) 76, 157, 827, 994, 1079, 1093, 1189, 1243
DraI TTTAAA 1 cut(s) 1176
DriI GACNNNNNGTC 1 cut(s) 805
Eam1104I CTCTTC 2 cut(s) 187, 918
Eam1105I GACNNNNNGTC 1 cut(s) 805
EarI CTCTTC 2 cut(s) 187, 918
EciI GGCGGA 2 cut(s) 157, 393
Ecl136II GAGCTC 4 cut(s) 313, 887, 943, 979
Eco130I CCWWGG 2 cut(s) 670, 946
Eco24I GRGCYC 4 cut(s) 315, 889, 945, 981
Eco53kI GAGCTC 4 cut(s) 313, 887, 943, 979
Eco57I CTGAAG 1 cut(s) 556
EcoICRI GAGCTC 4 cut(s) 313, 887, 943, 979
EcoRII CCWGG 2 cut(s) 890, 1235
EcoT14I CCWWGG 2 cut(s) 670, 946
EcoT38I GRGCYC 4 cut(s) 315, 889, 945, 981
EgeI GGCGCC 2 cut(s) 226, 552
EheI GGCGCC 2 cut(s) 226, 552
ErhI CCWWGG 2 cut(s) 670, 946
FaeI CATG 9 cut(s) 357, 395, 610, 616, 826, 883, 935, 985, 1013
FalI AAGNNNNNCTT 1 cut(s) 35
FatI CATG 9 cut(s) 353, 391, 606, 612, 822, 879, 931, 981, 1009
FauNDI CATATG 2 cut(s) 185, 1194
FblI GTMKAC 1 cut(s) 1103
FokI GGATG 4 cut(s) 112, 907, 998, 1100
FriOI GRGCYC 4 cut(s) 315, 889, 945, 981
FspBI CTAG 5 cut(s) 411, 492, 671, 831, 1064
GlaI GCGC 3 cut(s) 226, 352, 552
GsaI CCCAGC 1 cut(s) 594
GsuI CTGGAG 3 cut(s) 84, 470, 1219
HaeII RGCGCY 2 cut(s) 228, 554
HaeIII GGCC 3 cut(s) 266, 335, 344
HapII CCGG 1 cut(s) 578
HhaI GCGC 3 cut(s) 227, 353, 553
Hin1I GRCGYC 2 cut(s) 225, 551
Hin1II CATG 9 cut(s) 357, 395, 610, 616, 826, 883, 935, 985, 1013
Hin6I GCGC 3 cut(s) 225, 351, 551
HinP1I GCGC 3 cut(s) 225, 351, 551
HinfI GANTC 7 cut(s) 64, 70, 164, 439, 456, 475, 742
HpaII CCGG 1 cut(s) 578
Hpy166II GTNNAC 5 cut(s) 388, 484, 541, 1104, 1142
Hpy188I TCNGA 5 cut(s) 28, 69, 340, 575, 1075
Hpy188III TCNNGA 6 cut(s) 8, 74, 101, 161, 823, 831
Hpy8I GTNNAC 5 cut(s) 388, 484, 541, 1104, 1142
Hpy99I CGWCG 1 cut(s) 206
HpyAV CCTTC 5 cut(s) 8, 392, 580, 985, 1218
HpyCH4III ACNGT 3 cut(s) 488, 662, 1042
HpyCH4IV ACGT 1 cut(s) 521
HpyCH4V TGCA 6 cut(s) 38, 138, 422, 688, 1027, 1169
HpyF3I CTNAG 4 cut(s) 309, 337, 756, 1180
HpySE526I ACGT 1 cut(s) 521
Hsp92I GRCGYC 2 cut(s) 225, 551
Hsp92II CATG 9 cut(s) 357, 395, 610, 616, 826, 883, 935, 985, 1013
HspAI GCGC 3 cut(s) 225, 351, 551
KasI GGCGCC 2 cut(s) 224, 550
Kzo9I GATC 8 cut(s) 76, 157, 827, 994, 1079, 1093, 1189, 1243
LmnI GCTCC 5 cut(s) 127, 892, 948, 976, 1157
Lsp1109I GCAGC 8 cut(s) 22, 38, 44, 161, 593, 700, 715, 772
LweI GCATC 4 cut(s) 289, 715, 772, 885
MaeI CTAG 5 cut(s) 411, 492, 671, 831, 1064
MaeII ACGT 1 cut(s) 521
MalI GATC 8 cut(s) 78, 159, 829, 996, 1081, 1095, 1191, 1245
MboI GATC 8 cut(s) 76, 157, 827, 994, 1079, 1093, 1189, 1243
MboII GAAGA 9 cut(s) 23, 204, 221, 273, 370, 373, 415, 905, 1238
MflI RGATCY 3 cut(s) 827, 994, 1079
MhlI GDGCHC 6 cut(s) 124, 315, 889, 945, 981, 1188
MluCI AATT 8 cut(s) 502, 565, 595, 618, 736, 787, 1123, 1129
Mly113I GGCGCC 2 cut(s) 225, 551
MlyI GAGTC 2 cut(s) 158, 450
MmeI TCCRAC 1 cut(s) 954
MroXI GAANNNNTTC 1 cut(s) 368
MseI TTAA 6 cut(s) 642, 665, 1014, 1044, 1146, 1175
MslI CAYNNNNRTG 3 cut(s) 611, 768, 986
MspA1I CMGCKG 4 cut(s) 35, 174, 255, 296
MspI CCGG 1 cut(s) 578
MspR9I CCNGG 2 cut(s) 892, 1237
MvaI CCWGG 2 cut(s) 892, 1237
NarI GGCGCC 2 cut(s) 225, 551
NdeI CATATG 2 cut(s) 185, 1194
NdeII GATC 8 cut(s) 76, 157, 827, 994, 1079, 1093, 1189, 1243
NlaIII CATG 9 cut(s) 357, 395, 610, 616, 826, 883, 935, 985, 1013
NlaIV GGNNCC 3 cut(s) 226, 552, 1159
NmeAIII GCCGAG 2 cut(s) 311, 370
PagI TCATGA 1 cut(s) 822
PaqCI CACCTGC 1 cut(s) 595
PdmI GAANNNNTTC 1 cut(s) 368
PfeI GAWTC 5 cut(s) 64, 70, 439, 475, 742
PleI GAGTC 2 cut(s) 158, 450
PluTI GGCGCC 2 cut(s) 228, 554
PpsI GAGTC 2 cut(s) 158, 450
Psp124BI GAGCTC 4 cut(s) 315, 889, 945, 981
Psp6I CCWGG 2 cut(s) 890, 1235
PspFI CCCAGC 1 cut(s) 590
PspGI CCWGG 2 cut(s) 890, 1235
PspN4I GGNNCC 3 cut(s) 226, 552, 1159
PspPI GGNCC 1 cut(s) 264
PstI CTGCAG 1 cut(s) 40
PstNI CAGNNNCTG 2 cut(s) 51, 587
PsuI RGATCY 3 cut(s) 827, 994, 1079
PvuII CAGCTG 3 cut(s) 35, 174, 255
RsaI GTAC 3 cut(s) 490, 499, 542
RsaNI GTAC 3 cut(s) 489, 498, 541
RseI CAYNNNNRTG 3 cut(s) 611, 768, 986
SacI GAGCTC 4 cut(s) 315, 889, 945, 981
SaqAI TTAA 6 cut(s) 642, 665, 1014, 1044, 1146, 1175
Sau3AI GATC 8 cut(s) 76, 157, 827, 994, 1079, 1093, 1189, 1243
Sau96I GGNCC 1 cut(s) 264
ScaI AGTACT 1 cut(s) 499
SchI GAGTC 2 cut(s) 158, 450
ScrFI CCNGG 2 cut(s) 892, 1237
SduI GDGCHC 6 cut(s) 124, 315, 889, 945, 981, 1188
SfaNI GCATC 4 cut(s) 289, 715, 772, 885
SfcI CTRYAG 1 cut(s) 36
SfiI GGCCNNNNNGGCC 1 cut(s) 341
SfoI GGCGCC 2 cut(s) 226, 552
SmiMI CAYNNNNRTG 3 cut(s) 611, 768, 986
SpeI ACTAGT 2 cut(s) 410, 491
Sse9I AATT 8 cut(s) 502, 565, 595, 618, 736, 787, 1123, 1129
SsiI CCGC 7 cut(s) 42, 168, 222, 294, 375, 378, 701
SspDI GGCGCC 2 cut(s) 224, 550
SspMI CTAG 5 cut(s) 411, 492, 671, 831, 1064
SstI GAGCTC 4 cut(s) 315, 889, 945, 981
StyD4I CCNGG 2 cut(s) 890, 1235
StyI CCWWGG 2 cut(s) 670, 946
TaaI ACNGT 3 cut(s) 488, 662, 1042
TaiI ACGT 1 cut(s) 524
TaqI TCGA 1 cut(s) 939
TaqII GACCGA 1 cut(s) 159
TasI AATT 8 cut(s) 502, 565, 595, 618, 736, 787, 1123, 1129
TatI WGTACW 1 cut(s) 497
TauI GCSGC 5 cut(s) 45, 225, 296, 378, 703
TfiI GAWTC 5 cut(s) 64, 70, 439, 475, 742
Tru1I TTAA 6 cut(s) 642, 665, 1014, 1044, 1146, 1175
Tru9I TTAA 6 cut(s) 642, 665, 1014, 1044, 1146, 1175
TscAI CASTG 2 cut(s) 313, 1057
TseI GCWGC 8 cut(s) 32, 35, 51, 174, 581, 688, 703, 760
TspDTI ATGAA 4 cut(s) 12, 808, 920, 1238
TspGWI ACGGA 3 cut(s) 432, 717, 958
TspRI CASTG 2 cut(s) 313, 1057
XapI RAATTY 1 cut(s) 1129
XbaI TCTAGA 1 cut(s) 830
XmaJI CCTAGG 1 cut(s) 670
XmiI GTMKAC 1 cut(s) 1103
XmnI GAANNNNTTC 1 cut(s) 368
XspI CTAG 5 cut(s) 411, 492, 671, 831, 1064
ZrmI AGTACT 1 cut(s) 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.