RchiOBHm_Chr7g0177811
MYB Family

Transcription repressor KAN1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0004135
Physical Location & Seq
Reverse (-)
633261 .. 637405
4145 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ15842

Sequence Viewer

Length: 1224 bp
ATGCCTCATAAAGGGATTTTGGAGTCTTTGAATCCGATTCCTGATCTTTCACTTCATATAAGTCCTCCAAACAGTGCTCCAAACGAACCTGCAGACTCTGCGGCCTTCAACATATGGCGGTGCAGAGACAACACCGACGTGCACGACGGCGGTCTAGTCAAATCTCACAGTGACGATGGCGCTACTACTCCACTCGCCGCAGACACTGAGCTTTCCCTTGCAAATCCCACTAGTGCTTCGGAGGCCGAGAGTGACAGCAACTCATGGAAGATGATGAAATCGAGCTATTTCGCAAGAGATCAGCAGCAAGCAGCGGCCGAAAAGCACAACAACATAAGTCACGGCCTTTCCATGCTTGATGTGGCCGAGCTAGGAATCAAACCCATCAAAGGAATTCCAGTTTATAACACCACTCCCGCAGCAGCTAATTGCTCCTTTCCGTTTTCTCCACCTAATTTGGATCCGTCTAGGGATATTTCAGCAGCTGCTAATTCCAAGCTTTGCTTCTACCAAACGCCGCCGCCGTATCTATCTCCTGCTGCACCCTTCAGACCCGGTGGTGGTGCTGCTATGGGAGCAGGAGTAGGAGGAGGGTTTGAGTCCAGGTTTAACGGGATAACTATGGACAGTCTAAGAGTGCCTCAACAACACTTACAGTATCTGAATCATCATCACCATCAACAACAGCAGCTGCTGCAGCATCAACACCAATGTGGAGTTGGGGTTGGGATTGGGATTGGAGGTCATCATCATCATCATCATCATGGTGATTTCTCTAATACTAACGGGTTCATCATGAGATCTAGATTTACGCCCAAGTCGTATCAGAGTAAGAGGAACATGAGGGCACCCAGAATGCGATGGACCAGCTCCCTCCACGCGCGTTTTGTTCACGCCGTCGAGCTCCTTGGCGGCCATGAAAGGGCGACTCCAAAGTCAGTGTTGGAGCTCATGGATGTCAAAGATCTCACACTTGCTCATGTTAAGAGCCATTTGCAGATGTATAGAACTGTGAAGAACACTGACAAACCTGTAGCTTCCTCAGATGGGTCTGGTGATGAAGATCTTATGTCCACAACAACGACAACGCATCATCAGAATTCAGCAAATGGTTTACTAAACCAAAGAGGAGGCTCAAATGCAAGTTTAAATTTAGAGCAAGATACGGAACACCCTTCCAGCAATCTGTGGGGTAACTCTTCCAGGTATGATCTTCATTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

407

Amino Acids

44.32

Weight (kDa)

6.74

Isoelectric Point (pI)

43.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 285 - 336 3e-06 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 405
AasI GACNNNNNNGTC 1 cut(s) 934
Acc36I ACCTGC 1 cut(s) 97
AccB1I GGYRCC 1 cut(s) 847
AccII CGCG 2 cut(s) 881, 883
AciI CCGC 9 cut(s) 101, 118, 150, 198, 314, 417, 518, 521, 912
AclWI GGATC 2 cut(s) 455, 468
AcoI YGGCCR 3 cut(s) 315, 363, 913
AcsI RAATTY 3 cut(s) 393, 1099, 1150
AcuI CTGAAG 1 cut(s) 532
AfiI CCNNNNNNNGG 5 cut(s) 11, 389, 560, 922, 1047
AgsI TTSAA 2 cut(s) 31, 109
AhlI ACTAGT 1 cut(s) 230
AjiI CACGTC 1 cut(s) 139
AjnI CCWGG 2 cut(s) 602, 1202
AjuI GAANNNNNNNTTGG 2 cut(s) 488, 520
AleI CACNNNNGTG 2 cut(s) 137, 711
Alw21I GWGCWC 4 cut(s) 79, 144, 906, 951
Alw26I GTCTC 1 cut(s) 120
Alw44I GTGCAC 1 cut(s) 140
AlwI GGATC 2 cut(s) 455, 468
AlwNI CAGNNNCTG 6 cut(s) 98, 206, 485, 661, 691, 694
AoxI GGCC 6 cut(s) 102, 243, 315, 343, 363, 913
ApaLI GTGCAC 1 cut(s) 140
ApoI RAATTY 3 cut(s) 393, 1099, 1150
AspLEI GCGC 2 cut(s) 182, 883
AspS9I GGNCC 1 cut(s) 864
AsuC2I CCSGG 1 cut(s) 555
AsuHPI GGTGA 3 cut(s) 665, 779, 1067
AvaII GGWCC 1 cut(s) 864
BaeGI GKGCMC 2 cut(s) 144, 850
BamHI GGATCC 1 cut(s) 460
BanI GGYRCC 1 cut(s) 847
BanII GRGCYC 2 cut(s) 906, 951
Bbv12I GWGCWC 4 cut(s) 79, 144, 906, 951
BccI CCATC 5 cut(s) 170, 392, 684, 855, 1040
BceAI ACGGC 4 cut(s) 163, 358, 508, 881
BciT130I CCWGG 2 cut(s) 604, 1204
BcnI CCSGG 1 cut(s) 555
BcoDI GTCTC 1 cut(s) 120
BcuI ACTAGT 1 cut(s) 230
BfaI CTAG 5 cut(s) 155, 231, 371, 468, 804
BfmI CTRYAG 3 cut(s) 90, 695, 1032
BfoI RGCGCY 1 cut(s) 183
BfuAI ACCTGC 1 cut(s) 97
BglII AGATCT 3 cut(s) 800, 964, 1063
Bme1390I CCNGG 3 cut(s) 555, 604, 1204
Bme18I GGWCC 1 cut(s) 864
BmgBI CACGTC 1 cut(s) 139
BmgT120I GGNCC 1 cut(s) 864
BmiI GGNNCC 2 cut(s) 462, 849
BmrFI CCNGG 3 cut(s) 555, 604, 1204
BmsI GCATC 2 cut(s) 709, 1099
BoxI GACNNNNGTC 1 cut(s) 150
BpuMI CCSGG 1 cut(s) 555
BsaBI GATNNNNATC 1 cut(s) 1062
BsaJI CCNNGG 1 cut(s) 907
BsaXI ACNNNNNCTCC 4 cut(s) 567, 597, 708, 738
Bsc4I CCNNNNNNNGG 5 cut(s) 11, 389, 560, 922, 1047
Bse1I ACTGG 1 cut(s) 398
Bse8I GATNNNNATC 1 cut(s) 1062
BseBI CCWGG 2 cut(s) 604, 1204
BseDI CCNNGG 1 cut(s) 907
BseGI GGATG 1 cut(s) 961
BseJI GATNNNNATC 1 cut(s) 1062
BseLI CCNNNNNNNGG 5 cut(s) 11, 389, 560, 922, 1047
BseMII CTCAG 2 cut(s) 198, 1056
BseNI ACTGG 1 cut(s) 398
BseRI GAGGAG 2 cut(s) 603, 1143
BseSI GKGCMC 2 cut(s) 144, 850
BseX3I CGGCCG 1 cut(s) 315
BsgI GTGCAG 2 cut(s) 142, 525
Bsh1236I CGCG 2 cut(s) 881, 883
Bsh1285I CGRYCG 1 cut(s) 318
BshFI GGCC 6 cut(s) 104, 245, 317, 345, 365, 915
BshNI GGYRCC 1 cut(s) 847
BsiEI CGRYCG 1 cut(s) 318
BsiHKAI GWGCWC 4 cut(s) 79, 144, 906, 951
BsiSI CCGG 1 cut(s) 555
BslI CCNNNNNNNGG 5 cut(s) 11, 389, 560, 922, 1047
BsmAI GTCTC 1 cut(s) 120
BsmI GAATGC 1 cut(s) 861
BsnI GGCC 6 cut(s) 104, 245, 317, 345, 365, 915
Bsp1286I GDGCHC 5 cut(s) 79, 144, 850, 906, 951
Bsp143I GATC 7 cut(s) 43, 298, 460, 800, 964, 1063, 1210
BspACI CCGC 9 cut(s) 101, 118, 150, 198, 314, 417, 518, 521, 912
BspANI GGCC 6 cut(s) 104, 245, 317, 345, 365, 915
BspCNI CTCAG 2 cut(s) 199, 1055
BspFNI CGCG 2 cut(s) 881, 883
BspHI TCATGA 1 cut(s) 795
BspLI GGNNCC 2 cut(s) 462, 849
BspMAI CTGCAG 2 cut(s) 94, 699
BspMI ACCTGC 1 cut(s) 97
BspPI GGATC 2 cut(s) 455, 468
BspT107I GGYRCC 1 cut(s) 847
BsrI ACTGG 1 cut(s) 398
BssECI CCNNGG 1 cut(s) 907
BssMI GATC 7 cut(s) 43, 298, 460, 800, 964, 1063, 1210
BssT1I CCWWGG 1 cut(s) 907
Bst2UI CCWGG 2 cut(s) 604, 1204
Bst4CI ACNGT 5 cut(s) 74, 170, 629, 657, 1012
Bst6I CTCTTC 1 cut(s) 1204
BstAPI GCANNNNNTGC 2 cut(s) 98, 694
BstC8I GCNNGC 1 cut(s) 309
BstDEI CTNAG 3 cut(s) 207, 632, 1042
BstENI CCTNNNNNAGG 1 cut(s) 9
BstF5I GGATG 1 cut(s) 961
BstFNI CGCG 2 cut(s) 881, 883
BstH2I RGCGCY 1 cut(s) 183
BstHHI GCGC 2 cut(s) 182, 883
BstKTI GATC 7 cut(s) 46, 301, 463, 803, 967, 1066, 1213
BstMAI GTCTC 1 cut(s) 120
BstMBI GATC 7 cut(s) 43, 298, 460, 800, 964, 1063, 1210
BstMCI CGRYCG 1 cut(s) 318
BstMWI GCNNNNNNNGC 5 cut(s) 98, 242, 575, 694, 697
BstNI CCWGG 2 cut(s) 604, 1204
BstPAI GACNNNNGTC 1 cut(s) 150
BstSCI CCNGG 3 cut(s) 553, 602, 1202
BstSFI CTRYAG 3 cut(s) 90, 695, 1032
BstSLI GKGCMC 2 cut(s) 144, 850
BstUI CGCG 2 cut(s) 881, 883
BstX2I RGATCY 4 cut(s) 460, 800, 964, 1063
BstYI RGATCY 4 cut(s) 460, 800, 964, 1063
BstZI CGGCCG 1 cut(s) 315
BsuRI GGCC 6 cut(s) 104, 245, 317, 345, 365, 915
BtgZI GCGATG 1 cut(s) 874
BtrI CACGTC 1 cut(s) 139
BtsCI GGATG 1 cut(s) 961
BtsIMutI CAGTG 5 cut(s) 79, 175, 204, 945, 1020
BveI ACCTGC 1 cut(s) 97
Cac8I GCNNGC 1 cut(s) 309
CaiI CAGNNNCTG 6 cut(s) 98, 206, 485, 661, 691, 694
CciI TCATGA 1 cut(s) 795
CfoI GCGC 2 cut(s) 182, 883
Cfr13I GGNCC 1 cut(s) 864
CspCI CAANNNNNGTGG 2 cut(s) 438, 473
CviAII CATG 8 cut(s) 264, 352, 764, 796, 841, 917, 952, 980
DdeI CTNAG 3 cut(s) 207, 632, 1042
DpnI GATC 7 cut(s) 45, 300, 462, 802, 966, 1065, 1212
DpnII GATC 7 cut(s) 43, 298, 460, 800, 964, 1063, 1210
DraI TTTAAA 1 cut(s) 1149
DrdI GACNNNNNNGTC 1 cut(s) 934
DseDI GACNNNNNNGTC 1 cut(s) 934
EaeI YGGCCR 3 cut(s) 315, 363, 913
EagI CGGCCG 1 cut(s) 315
Eam1104I CTCTTC 1 cut(s) 1204
EarI CTCTTC 1 cut(s) 1204
Ecl136II GAGCTC 2 cut(s) 904, 949
EclXI CGGCCG 1 cut(s) 315
Eco130I CCWWGG 1 cut(s) 907
Eco24I GRGCYC 2 cut(s) 906, 951
Eco47I GGWCC 1 cut(s) 864
Eco52I CGGCCG 1 cut(s) 315
Eco53kI GAGCTC 2 cut(s) 904, 949
Eco57I CTGAAG 1 cut(s) 532
EcoICRI GAGCTC 2 cut(s) 904, 949
EcoNI CCTNNNNNAGG 1 cut(s) 9
EcoRI GAATTC 2 cut(s) 393, 1099
EcoRII CCWGG 2 cut(s) 602, 1202
EcoT14I CCWWGG 1 cut(s) 907
EcoT38I GRGCYC 2 cut(s) 906, 951
ErhI CCWWGG 1 cut(s) 907
FaeI CATG 8 cut(s) 267, 355, 767, 799, 844, 920, 955, 983
FalI AAGNNNNNCTT 2 cut(s) 488, 520
FatI CATG 8 cut(s) 263, 351, 763, 795, 840, 916, 951, 979
FauI CCCGC 1 cut(s) 424
FauNDI CATATG 1 cut(s) 113
FokI GGATG 1 cut(s) 968
FriOI GRGCYC 2 cut(s) 906, 951
FspBI CTAG 5 cut(s) 155, 231, 371, 468, 804
GlaI GCGC 2 cut(s) 181, 882
HaeII RGCGCY 1 cut(s) 183
HaeIII GGCC 6 cut(s) 104, 245, 317, 345, 365, 915
HapII CCGG 1 cut(s) 555
HhaI GCGC 2 cut(s) 182, 883
Hin1II CATG 8 cut(s) 267, 355, 767, 799, 844, 920, 955, 983
Hin6I GCGC 2 cut(s) 180, 881
HinP1I GCGC 2 cut(s) 180, 881
HindIII AAGCTT 1 cut(s) 497
HinfI GANTC 8 cut(s) 23, 31, 37, 95, 375, 599, 664, 928
HpaII CCGG 1 cut(s) 555
HphI GGTGA 3 cut(s) 665, 779, 1067
Hpy166II GTNNAC 4 cut(s) 142, 892, 1074, 1115
Hpy188I TCNGA 7 cut(s) 36, 241, 551, 663, 828, 1045, 1098
Hpy188III TCNNGA 3 cut(s) 41, 796, 804
Hpy8I GTNNAC 4 cut(s) 142, 892, 1074, 1115
Hpy99I CGWCG 3 cut(s) 140, 149, 902
HpyAV CCTTC 3 cut(s) 115, 556, 1185
HpyCH4III ACNGT 5 cut(s) 74, 170, 629, 657, 1012
HpyCH4IV ACGT 1 cut(s) 138
HpyCH4V TGCA 8 cut(s) 92, 123, 142, 221, 542, 697, 997, 1142
HpyF10VI GCNNNNNNNGC 5 cut(s) 98, 242, 575, 694, 697
HpyF3I CTNAG 3 cut(s) 207, 632, 1042
HpySE526I ACGT 1 cut(s) 138
Hsp92II CATG 8 cut(s) 267, 355, 767, 799, 844, 920, 955, 983
HspAI GCGC 2 cut(s) 180, 881
Kzo9I GATC 7 cut(s) 43, 298, 460, 800, 964, 1063, 1210
LmnI GCTCC 6 cut(s) 82, 437, 575, 875, 909, 946
LweI GCATC 2 cut(s) 709, 1099
MaeI CTAG 5 cut(s) 155, 231, 371, 468, 804
MaeII ACGT 1 cut(s) 138
MaeIII GTNAC 4 cut(s) 170, 251, 338, 1193
MalI GATC 7 cut(s) 45, 300, 462, 802, 966, 1065, 1212
MboI GATC 7 cut(s) 43, 298, 460, 800, 964, 1063, 1210
MboII GAAGA 5 cut(s) 280, 1027, 1073, 1191, 1205
MflI RGATCY 4 cut(s) 460, 800, 964, 1063
MhlI GDGCHC 5 cut(s) 79, 144, 850, 906, 951
MluCI AATT 6 cut(s) 393, 427, 454, 490, 1099, 1150
MlyI GAGTC 4 cut(s) 32, 89, 608, 922
MmeI TCCRAC 1 cut(s) 924
MseI TTAA 3 cut(s) 609, 984, 1148
MslI CAYNNNNRTG 4 cut(s) 137, 711, 762, 765
MspA1I CMGCKG 3 cut(s) 314, 485, 691
MspI CCGG 1 cut(s) 555
MspR9I CCNGG 3 cut(s) 555, 604, 1204
Mva1269I GAATGC 1 cut(s) 861
MvaI CCWGG 2 cut(s) 604, 1204
MvnI CGCG 2 cut(s) 881, 883
MwoI GCNNNNNNNGC 5 cut(s) 98, 242, 575, 694, 697
NciI CCSGG 1 cut(s) 555
NdeI CATATG 1 cut(s) 113
NdeII GATC 7 cut(s) 43, 298, 460, 800, 964, 1063, 1210
NlaIII CATG 8 cut(s) 267, 355, 767, 799, 844, 920, 955, 983
NlaIV GGNNCC 2 cut(s) 462, 849
NmeAIII GCCGAG 2 cut(s) 271, 391
NmuCI GTSAC 3 cut(s) 170, 251, 338
OliI CACNNNNGTG 2 cut(s) 137, 711
PagI TCATGA 1 cut(s) 795
PcsI WCGNNNNNNNCGW 2 cut(s) 521, 818
PctI GAATGC 1 cut(s) 861
PfeI GAWTC 4 cut(s) 31, 37, 375, 664
PleI GAGTC 4 cut(s) 31, 89, 607, 922
PpsI GAGTC 4 cut(s) 31, 89, 607, 922
PshAI GACNNNNGTC 1 cut(s) 150
PsiI TTATAA 1 cut(s) 405
Psp124BI GAGCTC 2 cut(s) 906, 951
Psp6I CCWGG 2 cut(s) 602, 1202
PspGI CCWGG 2 cut(s) 602, 1202
PspN4I GGNNCC 2 cut(s) 462, 849
PspPI GGNCC 1 cut(s) 864
PstI CTGCAG 2 cut(s) 94, 699
PstNI CAGNNNCTG 6 cut(s) 98, 206, 485, 661, 691, 694
PsuI RGATCY 4 cut(s) 460, 800, 964, 1063
PvuII CAGCTG 2 cut(s) 485, 691
RseI CAYNNNNRTG 4 cut(s) 137, 711, 762, 765
SacI GAGCTC 2 cut(s) 906, 951
SaqAI TTAA 3 cut(s) 609, 984, 1148
Sau3AI GATC 7 cut(s) 43, 298, 460, 800, 964, 1063, 1210
Sau96I GGNCC 1 cut(s) 864
SchI GAGTC 4 cut(s) 32, 89, 608, 922
ScrFI CCNGG 3 cut(s) 555, 604, 1204
SduI GDGCHC 5 cut(s) 79, 144, 850, 906, 951
SfaNI GCATC 2 cut(s) 709, 1099
SfcI CTRYAG 3 cut(s) 90, 695, 1032
SinI GGWCC 1 cut(s) 864
SmiMI CAYNNNNRTG 4 cut(s) 137, 711, 762, 765
SpeI ACTAGT 1 cut(s) 230
Sse9I AATT 6 cut(s) 393, 427, 454, 490, 1099, 1150
SsiI CCGC 9 cut(s) 101, 118, 150, 198, 314, 417, 518, 521, 912
SspMI CTAG 5 cut(s) 155, 231, 371, 468, 804
SstI GAGCTC 2 cut(s) 906, 951
StyD4I CCNGG 3 cut(s) 553, 602, 1202
StyI CCWWGG 1 cut(s) 907
TaaI ACNGT 5 cut(s) 74, 170, 629, 657, 1012
TaiI ACGT 1 cut(s) 141
TaqI TCGA 2 cut(s) 281, 900
TasI AATT 6 cut(s) 393, 427, 454, 490, 1099, 1150
TauI GCSGC 6 cut(s) 104, 200, 317, 520, 523, 915
TfiI GAWTC 4 cut(s) 31, 37, 375, 664
Tru1I TTAA 3 cut(s) 609, 984, 1148
Tru9I TTAA 3 cut(s) 609, 984, 1148
TscAI CASTG 5 cut(s) 79, 175, 211, 945, 1027
TseFI GTSAC 3 cut(s) 170, 251, 338
Tsp45I GTSAC 3 cut(s) 170, 251, 338
TspDTI ATGAA 6 cut(s) 44, 290, 781, 933, 1074, 1205
TspGWI ACGGA 3 cut(s) 429, 453, 1181
TspRI CASTG 5 cut(s) 79, 175, 211, 945, 1027
VneI GTGCAC 1 cut(s) 140
VpaK11BI GGWCC 1 cut(s) 864
XagI CCTNNNNNAGG 1 cut(s) 9
XapI RAATTY 3 cut(s) 393, 1099, 1150
XbaI TCTAGA 1 cut(s) 803
XcmI CCANNNNNNNNNTGG 2 cut(s) 358, 716
XspI CTAG 5 cut(s) 155, 231, 371, 468, 804
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.