MD07G1111400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003173
Physical Location & Seq
Forward (+)
12787898 .. 12790676
2779 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1111400.v1.1.491

Sequence Viewer

Length: 501 bp
ATGGATGAGACCATGAAGCAGTTCCAGAAGAGCTTAATTGAGCTCGAGACTGAAGCTGAGCATCTCCTTCTAGCTCGAAACCAGATGGTTGAAAATGACAGGGTGAGGAATGGGAACAGAGAAGCACTTACATCTCTGAGGAAGATAGCTCGGACAACCAAAACTAGCGTTCCATCTCCTTTCGAGTCAATAATGAAGGAGATTGGGGGCCCTCAATCAAGACCTCTGGTGAAGGAGGTATGCCCTACCTGTGGCAACCATGACTCAAATGACCGTACTTGGATGATGTTCCCAGGAACTGATGTCTTTGCAAGGATTCCATTTCATGCTGCCCATACCATCTTGGAGACAGAACAAGAACAACTTGATTTAGACTCTAGAAGGTTGCAAGGCATTGTGAAGGAGAAATCCCTTGTGATTTCGGGCAAAGGTGCCCTTGCTGATAAGATCTGTCCGGGTGTGCTGAAGTCCTTGGTAACCCTGACAGACAAACCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

167

Amino Acids

18.63

Weight (kDa)

7.74

Isoelectric Point (pI)

40.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 431
AcuI CTGAAG 2 cut(s) 72, 485
AfaI GTAC 1 cut(s) 277
AfiI CCNNNNNNNGG 1 cut(s) 251
AgsI TTSAA 1 cut(s) 92
AjnI CCWGG 1 cut(s) 292
AluBI AGCT 5 cut(s) 33, 43, 56, 74, 149
AluI AGCT 5 cut(s) 33, 43, 56, 74, 149
Alw21I GWGCWC 1 cut(s) 45
Alw26I GTCTC 3 cut(s) 2, 41, 341
AlwNI CAGNNNCTG 1 cut(s) 299
Ama87I CYCGRG 1 cut(s) 44
AoxI GGCC 1 cut(s) 208
ApaI GGGCCC 1 cut(s) 212
ApeKI GCWGC 1 cut(s) 329
Asp700I GAANNNNTTC 1 cut(s) 20
AspS9I GGNCC 2 cut(s) 208, 209
AsuC2I CCSGG 1 cut(s) 456
AsuHPI GGTGA 2 cut(s) 115, 241
AvaI CYCGRG 1 cut(s) 44
BaeGI GKGCMC 2 cut(s) 212, 436
BanI GGYRCC 1 cut(s) 431
BanII GRGCYC 2 cut(s) 45, 212
Bbv12I GWGCWC 1 cut(s) 45
BbvI GCAGC 1 cut(s) 316
BccI CCATC 3 cut(s) 79, 181, 347
BciT130I CCWGG 1 cut(s) 294
BcnI CCSGG 1 cut(s) 456
BcoDI GTCTC 3 cut(s) 2, 41, 341
BfaI CTAG 3 cut(s) 71, 165, 378
BglII AGATCT 1 cut(s) 447
BisI GCNGC 1 cut(s) 330
BlpI GCTNAGC 1 cut(s) 57
BlsI GCNGC 1 cut(s) 331
Bme1390I CCNGG 2 cut(s) 294, 456
BmeT110I CYCGRG 1 cut(s) 44
BmgT120I GGNCC 2 cut(s) 208, 209
BmiI GGNNCC 3 cut(s) 209, 210, 433
BmrFI CCNGG 2 cut(s) 294, 456
BmsI GCATC 1 cut(s) 70
Bpu1102I GCTNAGC 1 cut(s) 57
BpuMI CCSGG 1 cut(s) 456
BsaI GGTCTC 1 cut(s) 2
BsaJI CCNNGG 2 cut(s) 292, 471
Bsc4I CCNNNNNNNGG 1 cut(s) 251
BseBI CCWGG 1 cut(s) 294
BseDI CCNNGG 2 cut(s) 292, 471
BseGI GGATG 2 cut(s) 10, 288
BseLI CCNNNNNNNGG 1 cut(s) 251
BseMII CTCAG 2 cut(s) 48, 128
BseSI GKGCMC 2 cut(s) 212, 436
BseXI GCAGC 1 cut(s) 316
BshFI GGCC 1 cut(s) 210
BshNI GGYRCC 1 cut(s) 431
BsiHKAI GWGCWC 1 cut(s) 45
BsiHKCI CYCGRG 1 cut(s) 44
BsiSI CCGG 1 cut(s) 455
BslI CCNNNNNNNGG 1 cut(s) 251
BsmAI GTCTC 3 cut(s) 2, 41, 341
BsnI GGCC 1 cut(s) 210
Bso31I GGTCTC 1 cut(s) 2
BsoBI CYCGRG 1 cut(s) 44
Bsp120I GGGCCC 1 cut(s) 208
Bsp1286I GDGCHC 3 cut(s) 45, 212, 436
Bsp143I GATC 1 cut(s) 447
Bsp1720I GCTNAGC 1 cut(s) 57
BspANI GGCC 1 cut(s) 210
BspCNI CTCAG 2 cut(s) 49, 129
BspLI GGNNCC 3 cut(s) 209, 210, 433
BspQI GCTCTTC 1 cut(s) 23
BspT107I GGYRCC 1 cut(s) 431
BspTNI GGTCTC 1 cut(s) 2
BssECI CCNNGG 2 cut(s) 292, 471
BssMI GATC 1 cut(s) 447
BssT1I CCWWGG 1 cut(s) 471
Bst2UI CCWGG 1 cut(s) 294
Bst4CI ACNGT 1 cut(s) 275
Bst6I CTCTTC 1 cut(s) 23
BstDEI CTNAG 2 cut(s) 57, 137
BstEII GGTNACC 1 cut(s) 475
BstF5I GGATG 2 cut(s) 10, 288
BstKTI GATC 1 cut(s) 450
BstMAI GTCTC 3 cut(s) 2, 41, 341
BstMBI GATC 1 cut(s) 447
BstNI CCWGG 1 cut(s) 294
BstPI GGTNACC 1 cut(s) 475
BstSCI CCNGG 2 cut(s) 292, 454
BstSLI GKGCMC 2 cut(s) 212, 436
BstV1I GCAGC 1 cut(s) 316
BstX2I RGATCY 1 cut(s) 447
BstYI RGATCY 1 cut(s) 447
BsuRI GGCC 1 cut(s) 210
BtsCI GGATG 2 cut(s) 10, 288
CaiI CAGNNNCTG 1 cut(s) 299
Cfr13I GGNCC 2 cut(s) 208, 209
Csp6I GTAC 1 cut(s) 276
CviAII CATG 3 cut(s) 13, 260, 326
CviJI RGCY 6 cut(s) 33, 43, 56, 74, 149, 210
CviKI_1 RGCY 6 cut(s) 33, 43, 56, 74, 149, 210
CviQI GTAC 1 cut(s) 276
DdeI CTNAG 2 cut(s) 57, 137
DpnI GATC 1 cut(s) 449
DpnII GATC 1 cut(s) 447
Eam1104I CTCTTC 1 cut(s) 23
EarI CTCTTC 1 cut(s) 23
Ecl136II GAGCTC 1 cut(s) 43
Eco130I CCWWGG 1 cut(s) 471
Eco24I GRGCYC 2 cut(s) 45, 212
Eco31I GGTCTC 1 cut(s) 2
Eco53kI GAGCTC 1 cut(s) 43
Eco57I CTGAAG 2 cut(s) 72, 485
Eco88I CYCGRG 1 cut(s) 44
Eco91I GGTNACC 1 cut(s) 475
EcoICRI GAGCTC 1 cut(s) 43
EcoO109I RGGNCCY 2 cut(s) 208, 209
EcoO65I GGTNACC 1 cut(s) 475
EcoRII CCWGG 1 cut(s) 292
EcoT14I CCWWGG 1 cut(s) 471
EcoT38I GRGCYC 2 cut(s) 45, 212
ErhI CCWWGG 1 cut(s) 471
FaeI CATG 3 cut(s) 16, 263, 329
FaiI YATR 5 cut(s) 14, 241, 261, 327, 336
FalI AAGNNNNNCTT 4 cut(s) 348, 380, 420, 452
FatI CATG 3 cut(s) 12, 259, 325
Fnu4HI GCNGC 1 cut(s) 330
FokI GGATG 2 cut(s) 17, 295
FriOI GRGCYC 2 cut(s) 45, 212
Fsp4HI GCNGC 1 cut(s) 330
FspBI CTAG 3 cut(s) 71, 165, 378
GluI GCNGC 1 cut(s) 330
HaeIII GGCC 1 cut(s) 210
HapII CCGG 1 cut(s) 455
Hin1II CATG 3 cut(s) 16, 263, 329
HinfI GANTC 4 cut(s) 185, 263, 316, 374
HpaII CCGG 1 cut(s) 455
HphI GGTGA 2 cut(s) 115, 241
Hpy188I TCNGA 2 cut(s) 138, 153
Hpy188III TCNNGA 4 cut(s) 25, 46, 219, 378
HpyAV CCTTC 5 cut(s) 77, 190, 226, 375, 394
HpyCH4III ACNGT 1 cut(s) 275
HpyCH4V TGCA 2 cut(s) 311, 388
HpyF3I CTNAG 2 cut(s) 57, 137
Hsp92II CATG 3 cut(s) 16, 263, 329
Kzo9I GATC 1 cut(s) 447
LguI GCTCTTC 1 cut(s) 23
LpnPI CCDG 9 cut(s) 38, 85, 95, 212, 262, 279, 306, 468, 494
Lsp1109I GCAGC 1 cut(s) 316
LweI GCATC 1 cut(s) 70
MaeI CTAG 3 cut(s) 71, 165, 378
MaeIII GTNAC 1 cut(s) 475
MalI GATC 1 cut(s) 449
MboI GATC 1 cut(s) 447
MboII GAAGA 2 cut(s) 40, 154
MflI RGATCY 1 cut(s) 447
MhlI GDGCHC 3 cut(s) 45, 212, 436
MluCI AATT 1 cut(s) 36
MlyI GAGTC 3 cut(s) 194, 257, 368
MnlI CCTC 5 cut(s) 99, 132, 222, 229, 234
MroXI GAANNNNTTC 1 cut(s) 20
MseI TTAA 1 cut(s) 35
MspI CCGG 1 cut(s) 455
MspR9I CCNGG 2 cut(s) 294, 456
MvaI CCWGG 1 cut(s) 294
NciI CCSGG 1 cut(s) 456
NdeII GATC 1 cut(s) 447
NlaIII CATG 3 cut(s) 16, 263, 329
NlaIV GGNNCC 3 cut(s) 209, 210, 433
PaeR7I CTCGAG 1 cut(s) 44
PciSI GCTCTTC 1 cut(s) 23
PdmI GAANNNNTTC 1 cut(s) 20
PfeI GAWTC 1 cut(s) 316
PkrI GCNGC 1 cut(s) 331
PleI GAGTC 3 cut(s) 193, 257, 368
PpsI GAGTC 3 cut(s) 193, 257, 368
Psp124BI GAGCTC 1 cut(s) 45
Psp6I CCWGG 1 cut(s) 292
PspEI GGTNACC 1 cut(s) 475
PspGI CCWGG 1 cut(s) 292
PspN4I GGNNCC 3 cut(s) 209, 210, 433
PspOMI GGGCCC 1 cut(s) 208
PspPI GGNCC 2 cut(s) 208, 209
PstNI CAGNNNCTG 1 cut(s) 299
PsuI RGATCY 1 cut(s) 447
RsaI GTAC 1 cut(s) 277
RsaNI GTAC 1 cut(s) 276
SacI GAGCTC 1 cut(s) 45
SapI GCTCTTC 1 cut(s) 23
SaqAI TTAA 1 cut(s) 35
SatI GCNGC 1 cut(s) 330
Sau3AI GATC 1 cut(s) 447
Sau96I GGNCC 2 cut(s) 208, 209
SchI GAGTC 3 cut(s) 194, 257, 368
ScrFI CCNGG 2 cut(s) 294, 456
SduI GDGCHC 3 cut(s) 45, 212, 436
SfaNI GCATC 1 cut(s) 70
Sfr274I CTCGAG 1 cut(s) 44
SlaI CTCGAG 1 cut(s) 44
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
Sse9I AATT 1 cut(s) 36
SspMI CTAG 3 cut(s) 71, 165, 378
SstI GAGCTC 1 cut(s) 45
StyD4I CCNGG 2 cut(s) 292, 454
StyI CCWWGG 1 cut(s) 471
TaaI ACNGT 1 cut(s) 275
TaqI TCGA 3 cut(s) 45, 76, 183
TasI AATT 1 cut(s) 36
TfiI GAWTC 1 cut(s) 316
Tru1I TTAA 1 cut(s) 35
Tru9I TTAA 1 cut(s) 35
TseI GCWGC 1 cut(s) 329
TspDTI ATGAA 3 cut(s) 29, 209, 314
XbaI TCTAGA 1 cut(s) 377
XhoI CTCGAG 1 cut(s) 44
XmnI GAANNNNTTC 1 cut(s) 20
XspI CTAG 3 cut(s) 71, 165, 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.