pycom01g05780

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0003173
Physical Location & Seq
Reverse (-)
5638896 .. 5640920
2025 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g05780.4

Sequence Viewer

Length: 504 bp
ATGGATGAGACCATGAAGCAATTCCAGAAAAGCTTAATTGAACTCGAGACTGAAGCTGAGCATCTCCTCCTAGCTCGAAACCAGATGGTTGAAAATGACAGGGTGAGGAATGGGAACAGAGAAGCACTTACATCTCTGAGGAAGATAGCTCGGACAACCAAAACTAGTGTTCCATCTCCTTTTGAGTCAATAATGAAGGAGATTGGGGGGCCTCAATCAAGACCTTTGGTGAAGGAGGTATGCCCTACCTGTGGCAACCATGATTCAAATGAGCGCACTTGGATGATGTTCCCGGGAACTGATGTCTTTGCAAGGATTCCATTTCATGCTGCCCATACCATCTTGGAGACAGAACAAGAGCAACTTGATTCAGACTCTAGAAGGTTGCAAGGCATTGTGAAGGAAAAATCCCTTGTGATTTCGGACAAAGGTGCCCTTGCTGACAAGATCTGTCCGGGTGTGCTGAAGTCCTTGGTAACCCTGACAGACAAACCAAAGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

168

Amino Acids

18.79

Weight (kDa)

6.44

Isoelectric Point (pI)

41.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 431
AcuI CTGAAG 2 cut(s) 72, 485
AfiI CCNNNNNNNGG 1 cut(s) 251
AgsI TTSAA 3 cut(s) 41, 92, 267
AhlI ACTAGT 1 cut(s) 164
AluBI AGCT 4 cut(s) 33, 56, 74, 149
AluI AGCT 4 cut(s) 33, 56, 74, 149
Alw26I GTCTC 3 cut(s) 2, 41, 341
Ama87I CYCGRG 2 cut(s) 44, 292
AoxI GGCC 1 cut(s) 209
ApeKI GCWGC 1 cut(s) 329
Asp700I GAANNNNTTC 1 cut(s) 20
AspLEI GCGC 1 cut(s) 276
AspS9I GGNCC 1 cut(s) 209
AsuC2I CCSGG 3 cut(s) 293, 294, 456
AsuHPI GGTGA 2 cut(s) 115, 241
AvaI CYCGRG 2 cut(s) 44, 292
BaeGI GKGCMC 1 cut(s) 436
BanI GGYRCC 1 cut(s) 431
BbvI GCAGC 1 cut(s) 316
BccI CCATC 3 cut(s) 79, 181, 347
BcnI CCSGG 3 cut(s) 293, 294, 456
BcoDI GTCTC 3 cut(s) 2, 41, 341
BcuI ACTAGT 1 cut(s) 164
BfaI CTAG 3 cut(s) 71, 165, 378
BglII AGATCT 1 cut(s) 447
BisI GCNGC 1 cut(s) 330
BlpI GCTNAGC 1 cut(s) 57
BlsI GCNGC 1 cut(s) 331
Bme1390I CCNGG 3 cut(s) 293, 294, 456
BmeT110I CYCGRG 2 cut(s) 44, 292
BmgT120I GGNCC 1 cut(s) 209
BmiI GGNNCC 2 cut(s) 210, 433
BmrFI CCNGG 3 cut(s) 293, 294, 456
BmsI GCATC 1 cut(s) 70
Bpu1102I GCTNAGC 1 cut(s) 57
BpuMI CCSGG 3 cut(s) 293, 294, 456
BsaI GGTCTC 1 cut(s) 2
BsaJI CCNNGG 2 cut(s) 292, 471
Bsc4I CCNNNNNNNGG 1 cut(s) 251
BseDI CCNNGG 2 cut(s) 292, 471
BseGI GGATG 2 cut(s) 10, 288
BseLI CCNNNNNNNGG 1 cut(s) 251
BseMII CTCAG 2 cut(s) 48, 128
BseRI GAGGAG 1 cut(s) 56
BseSI GKGCMC 1 cut(s) 436
BseXI GCAGC 1 cut(s) 316
BshFI GGCC 1 cut(s) 211
BshNI GGYRCC 1 cut(s) 431
BsiHKCI CYCGRG 2 cut(s) 44, 292
BsiSI CCGG 2 cut(s) 293, 455
BslI CCNNNNNNNGG 1 cut(s) 251
BsmAI GTCTC 3 cut(s) 2, 41, 341
BsnI GGCC 1 cut(s) 211
Bso31I GGTCTC 1 cut(s) 2
BsoBI CYCGRG 2 cut(s) 44, 292
Bsp1286I GDGCHC 1 cut(s) 436
Bsp143I GATC 1 cut(s) 447
Bsp1720I GCTNAGC 1 cut(s) 57
BspANI GGCC 1 cut(s) 211
BspCNI CTCAG 2 cut(s) 49, 129
BspLI GGNNCC 2 cut(s) 210, 433
BspT107I GGYRCC 1 cut(s) 431
BspTNI GGTCTC 1 cut(s) 2
BssECI CCNNGG 2 cut(s) 292, 471
BssMI GATC 1 cut(s) 447
BssT1I CCWWGG 1 cut(s) 471
BstDEI CTNAG 2 cut(s) 57, 137
BstEII GGTNACC 1 cut(s) 475
BstF5I GGATG 2 cut(s) 10, 288
BstHHI GCGC 1 cut(s) 276
BstKTI GATC 1 cut(s) 450
BstMAI GTCTC 3 cut(s) 2, 41, 341
BstMBI GATC 1 cut(s) 447
BstPI GGTNACC 1 cut(s) 475
BstSCI CCNGG 3 cut(s) 291, 292, 454
BstSLI GKGCMC 1 cut(s) 436
BstV1I GCAGC 1 cut(s) 316
BstX2I RGATCY 1 cut(s) 447
BstYI RGATCY 1 cut(s) 447
BsuRI GGCC 1 cut(s) 211
BtsCI GGATG 2 cut(s) 10, 288
CfoI GCGC 1 cut(s) 276
Cfr13I GGNCC 1 cut(s) 209
Cfr9I CCCGGG 1 cut(s) 292
CviAII CATG 3 cut(s) 13, 260, 326
CviJI RGCY 5 cut(s) 33, 56, 74, 149, 211
CviKI_1 RGCY 5 cut(s) 33, 56, 74, 149, 211
DdeI CTNAG 2 cut(s) 57, 137
DpnI GATC 1 cut(s) 449
DpnII GATC 1 cut(s) 447
Eco130I CCWWGG 1 cut(s) 471
Eco31I GGTCTC 1 cut(s) 2
Eco57I CTGAAG 2 cut(s) 72, 485
Eco88I CYCGRG 2 cut(s) 44, 292
Eco91I GGTNACC 1 cut(s) 475
EcoO109I RGGNCCY 1 cut(s) 209
EcoO65I GGTNACC 1 cut(s) 475
EcoT14I CCWWGG 1 cut(s) 471
ErhI CCWWGG 1 cut(s) 471
FaeI CATG 3 cut(s) 16, 263, 329
FaiI YATR 5 cut(s) 14, 241, 261, 327, 336
FalI AAGNNNNNCTT 4 cut(s) 348, 380, 420, 452
FatI CATG 3 cut(s) 12, 259, 325
Fnu4HI GCNGC 1 cut(s) 330
FokI GGATG 2 cut(s) 17, 295
Fsp4HI GCNGC 1 cut(s) 330
FspBI CTAG 3 cut(s) 71, 165, 378
GlaI GCGC 1 cut(s) 275
GluI GCNGC 1 cut(s) 330
HaeIII GGCC 1 cut(s) 211
HapII CCGG 2 cut(s) 293, 455
HhaI GCGC 1 cut(s) 276
Hin1II CATG 3 cut(s) 16, 263, 329
Hin6I GCGC 1 cut(s) 274
HinP1I GCGC 1 cut(s) 274
HindIII AAGCTT 1 cut(s) 31
HinfI GANTC 5 cut(s) 185, 263, 316, 368, 374
HpaII CCGG 2 cut(s) 293, 455
HphI GGTGA 2 cut(s) 115, 241
Hpy188I TCNGA 4 cut(s) 138, 153, 373, 424
Hpy188III TCNNGA 4 cut(s) 25, 46, 219, 378
HpyAV CCTTC 4 cut(s) 190, 226, 375, 394
HpyCH4V TGCA 2 cut(s) 311, 388
HpyF3I CTNAG 2 cut(s) 57, 137
Hsp92II CATG 3 cut(s) 16, 263, 329
HspAI GCGC 1 cut(s) 274
Kzo9I GATC 1 cut(s) 447
LpnPI CCDG 7 cut(s) 38, 85, 95, 262, 306, 468, 494
Lsp1109I GCAGC 1 cut(s) 316
LweI GCATC 1 cut(s) 70
MaeI CTAG 3 cut(s) 71, 165, 378
MaeIII GTNAC 1 cut(s) 475
MalI GATC 1 cut(s) 449
MboI GATC 1 cut(s) 447
MboII GAAGA 1 cut(s) 154
MflI RGATCY 1 cut(s) 447
MhlI GDGCHC 1 cut(s) 436
MluCI AATT 2 cut(s) 20, 36
MlyI GAGTC 2 cut(s) 194, 368
MnlI CCTC 5 cut(s) 77, 99, 132, 222, 229
MroXI GAANNNNTTC 1 cut(s) 20
MseI TTAA 1 cut(s) 35
MslI CAYNNNNRTG 1 cut(s) 281
MspI CCGG 2 cut(s) 293, 455
MspR9I CCNGG 3 cut(s) 293, 294, 456
NciI CCSGG 3 cut(s) 293, 294, 456
NdeII GATC 1 cut(s) 447
NlaIII CATG 3 cut(s) 16, 263, 329
NlaIV GGNNCC 2 cut(s) 210, 433
PaeR7I CTCGAG 1 cut(s) 44
PdmI GAANNNNTTC 1 cut(s) 20
PfeI GAWTC 3 cut(s) 263, 316, 368
PkrI GCNGC 1 cut(s) 331
PleI GAGTC 2 cut(s) 193, 368
PpsI GAGTC 2 cut(s) 193, 368
PspEI GGTNACC 1 cut(s) 475
PspN4I GGNNCC 2 cut(s) 210, 433
PspPI GGNCC 1 cut(s) 209
PsuI RGATCY 1 cut(s) 447
RseI CAYNNNNRTG 1 cut(s) 281
SaqAI TTAA 1 cut(s) 35
SatI GCNGC 1 cut(s) 330
Sau3AI GATC 1 cut(s) 447
Sau96I GGNCC 1 cut(s) 209
SchI GAGTC 2 cut(s) 194, 368
ScrFI CCNGG 3 cut(s) 293, 294, 456
SduI GDGCHC 1 cut(s) 436
SetI ASST 9 cut(s) 35, 58, 76, 151, 226, 240, 251, 386, 433
SfaNI GCATC 1 cut(s) 70
Sfr274I CTCGAG 1 cut(s) 44
SlaI CTCGAG 1 cut(s) 44
SmaI CCCGGG 1 cut(s) 294
SmiMI CAYNNNNRTG 1 cut(s) 281
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
SpeI ACTAGT 1 cut(s) 164
Sse9I AATT 2 cut(s) 20, 36
SspMI CTAG 3 cut(s) 71, 165, 378
StyD4I CCNGG 3 cut(s) 291, 292, 454
StyI CCWWGG 1 cut(s) 471
TaqI TCGA 2 cut(s) 45, 76
TasI AATT 2 cut(s) 20, 36
TfiI GAWTC 3 cut(s) 263, 316, 368
Tru1I TTAA 1 cut(s) 35
Tru9I TTAA 1 cut(s) 35
TseI GCWGC 1 cut(s) 329
TspDTI ATGAA 3 cut(s) 29, 209, 314
TspMI CCCGGG 1 cut(s) 292
XbaI TCTAGA 1 cut(s) 377
XhoI CTCGAG 1 cut(s) 44
XmaI CCCGGG 1 cut(s) 292
XmnI GAANNNNTTC 1 cut(s) 20
XspI CTAG 3 cut(s) 71, 165, 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.