MD10G1232800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0003173
Physical Location & Seq
Forward (+)
32915864 .. 32921828
5965 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1232800.v1.1.491

Sequence Viewer

Length: 501 bp
ATGGATGAGACCATGAAGCAGTTCCAGAAAAGCTTAATTGAGCTCGAGACTGAAGCTGAGCATCTCCTTCTAGCTCGAAACCAGATGGTTGAAAATGACAGGGTGAGGAATGGGAACAGAGAAGCACTTACATCTCTGAGGAAGATAGCTCGGACAACCAAAACTAGCGTTCCATCTCCTTTCGAGTCAATAATGAAGGAGATTGGAGGGCCTCAATCAAGACCTCTGGTGAAGGAGGTATGCCCTACCTGTGGCAACCATGACTCAAATGACCGTACTTGGATGATGTTCCCGGGAACTGATGTCTTTGCAAGGATTCCATTTCATGCTGCCCATACCATCTTGGAGACAGAACAAGAACAACTTGATTTAGACTCTAGAAGGTTGCAAGGCATTGTGAAGGAGAAATCCCTTGTGATTTCGGGCAAAGGTGCCCTTGCTGATAAGATCTGTCCGGGTGTGCTGAAGTCCTTGGTAACCCTGACAGACAAACCAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

167

Amino Acids

18.66

Weight (kDa)

7.75

Isoelectric Point (pI)

39.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 431
AcuI CTGAAG 2 cut(s) 72, 485
AfaI GTAC 1 cut(s) 277
AfiI CCNNNNNNNGG 1 cut(s) 251
AgsI TTSAA 1 cut(s) 92
AluBI AGCT 5 cut(s) 33, 43, 56, 74, 149
AluI AGCT 5 cut(s) 33, 43, 56, 74, 149
Alw21I GWGCWC 1 cut(s) 45
Alw26I GTCTC 3 cut(s) 2, 41, 341
Ama87I CYCGRG 2 cut(s) 44, 292
AoxI GGCC 1 cut(s) 209
ApeKI GCWGC 1 cut(s) 329
Asp700I GAANNNNTTC 1 cut(s) 20
AspS9I GGNCC 1 cut(s) 209
AsuC2I CCSGG 3 cut(s) 293, 294, 456
AsuHPI GGTGA 2 cut(s) 115, 241
AvaI CYCGRG 2 cut(s) 44, 292
BaeGI GKGCMC 1 cut(s) 436
BanI GGYRCC 1 cut(s) 431
BanII GRGCYC 1 cut(s) 45
Bbv12I GWGCWC 1 cut(s) 45
BbvI GCAGC 1 cut(s) 316
BccI CCATC 3 cut(s) 79, 181, 347
BcnI CCSGG 3 cut(s) 293, 294, 456
BcoDI GTCTC 3 cut(s) 2, 41, 341
BfaI CTAG 3 cut(s) 71, 165, 378
BglII AGATCT 1 cut(s) 447
BisI GCNGC 1 cut(s) 330
BlpI GCTNAGC 1 cut(s) 57
BlsI GCNGC 1 cut(s) 331
Bme1390I CCNGG 3 cut(s) 293, 294, 456
BmeT110I CYCGRG 2 cut(s) 44, 292
BmgT120I GGNCC 1 cut(s) 209
BmiI GGNNCC 1 cut(s) 433
BmrFI CCNGG 3 cut(s) 293, 294, 456
BmsI GCATC 1 cut(s) 70
Bpu1102I GCTNAGC 1 cut(s) 57
BpuMI CCSGG 3 cut(s) 293, 294, 456
BsaI GGTCTC 1 cut(s) 2
BsaJI CCNNGG 3 cut(s) 292, 471, 494
Bsc4I CCNNNNNNNGG 1 cut(s) 251
BseDI CCNNGG 3 cut(s) 292, 471, 494
BseGI GGATG 2 cut(s) 10, 288
BseLI CCNNNNNNNGG 1 cut(s) 251
BseMII CTCAG 2 cut(s) 48, 128
BseSI GKGCMC 1 cut(s) 436
BseXI GCAGC 1 cut(s) 316
BshFI GGCC 1 cut(s) 211
BshNI GGYRCC 1 cut(s) 431
BsiHKAI GWGCWC 1 cut(s) 45
BsiHKCI CYCGRG 2 cut(s) 44, 292
BsiSI CCGG 2 cut(s) 293, 455
BslI CCNNNNNNNGG 1 cut(s) 251
BsmAI GTCTC 3 cut(s) 2, 41, 341
BsnI GGCC 1 cut(s) 211
Bso31I GGTCTC 1 cut(s) 2
BsoBI CYCGRG 2 cut(s) 44, 292
Bsp1286I GDGCHC 2 cut(s) 45, 436
Bsp143I GATC 1 cut(s) 447
Bsp1720I GCTNAGC 1 cut(s) 57
BspANI GGCC 1 cut(s) 211
BspCNI CTCAG 2 cut(s) 49, 129
BspLI GGNNCC 1 cut(s) 433
BspT107I GGYRCC 1 cut(s) 431
BspTNI GGTCTC 1 cut(s) 2
BssECI CCNNGG 3 cut(s) 292, 471, 494
BssMI GATC 1 cut(s) 447
BssT1I CCWWGG 2 cut(s) 471, 494
Bst4CI ACNGT 1 cut(s) 275
BstDEI CTNAG 2 cut(s) 57, 137
BstEII GGTNACC 1 cut(s) 475
BstF5I GGATG 2 cut(s) 10, 288
BstKTI GATC 1 cut(s) 450
BstMAI GTCTC 3 cut(s) 2, 41, 341
BstMBI GATC 1 cut(s) 447
BstPI GGTNACC 1 cut(s) 475
BstSCI CCNGG 3 cut(s) 291, 292, 454
BstSLI GKGCMC 1 cut(s) 436
BstV1I GCAGC 1 cut(s) 316
BstX2I RGATCY 1 cut(s) 447
BstYI RGATCY 1 cut(s) 447
BsuRI GGCC 1 cut(s) 211
BtsCI GGATG 2 cut(s) 10, 288
Cfr13I GGNCC 1 cut(s) 209
Cfr9I CCCGGG 1 cut(s) 292
Csp6I GTAC 1 cut(s) 276
CviAII CATG 3 cut(s) 13, 260, 326
CviJI RGCY 6 cut(s) 33, 43, 56, 74, 149, 211
CviKI_1 RGCY 6 cut(s) 33, 43, 56, 74, 149, 211
CviQI GTAC 1 cut(s) 276
DdeI CTNAG 2 cut(s) 57, 137
DpnI GATC 1 cut(s) 449
DpnII GATC 1 cut(s) 447
Ecl136II GAGCTC 1 cut(s) 43
Eco130I CCWWGG 2 cut(s) 471, 494
Eco24I GRGCYC 1 cut(s) 45
Eco31I GGTCTC 1 cut(s) 2
Eco53kI GAGCTC 1 cut(s) 43
Eco57I CTGAAG 2 cut(s) 72, 485
Eco88I CYCGRG 2 cut(s) 44, 292
Eco91I GGTNACC 1 cut(s) 475
EcoICRI GAGCTC 1 cut(s) 43
EcoO109I RGGNCCY 1 cut(s) 209
EcoO65I GGTNACC 1 cut(s) 475
EcoT14I CCWWGG 2 cut(s) 471, 494
EcoT38I GRGCYC 1 cut(s) 45
ErhI CCWWGG 2 cut(s) 471, 494
FaeI CATG 3 cut(s) 16, 263, 329
FaiI YATR 5 cut(s) 14, 241, 261, 327, 336
FalI AAGNNNNNCTT 4 cut(s) 348, 380, 420, 452
FatI CATG 3 cut(s) 12, 259, 325
Fnu4HI GCNGC 1 cut(s) 330
FokI GGATG 2 cut(s) 17, 295
FriOI GRGCYC 1 cut(s) 45
Fsp4HI GCNGC 1 cut(s) 330
FspBI CTAG 3 cut(s) 71, 165, 378
GluI GCNGC 1 cut(s) 330
HaeIII GGCC 1 cut(s) 211
HapII CCGG 2 cut(s) 293, 455
Hin1II CATG 3 cut(s) 16, 263, 329
HindIII AAGCTT 1 cut(s) 31
HinfI GANTC 4 cut(s) 185, 263, 316, 374
HpaII CCGG 2 cut(s) 293, 455
HphI GGTGA 2 cut(s) 115, 241
Hpy188I TCNGA 2 cut(s) 138, 153
Hpy188III TCNNGA 4 cut(s) 25, 46, 219, 378
HpyAV CCTTC 5 cut(s) 77, 190, 226, 375, 394
HpyCH4III ACNGT 1 cut(s) 275
HpyCH4V TGCA 2 cut(s) 311, 388
HpyF3I CTNAG 2 cut(s) 57, 137
Hsp92II CATG 3 cut(s) 16, 263, 329
Kzo9I GATC 1 cut(s) 447
LpnPI CCDG 8 cut(s) 38, 85, 95, 212, 262, 306, 468, 494
Lsp1109I GCAGC 1 cut(s) 316
LweI GCATC 1 cut(s) 70
MaeI CTAG 3 cut(s) 71, 165, 378
MaeIII GTNAC 1 cut(s) 475
MalI GATC 1 cut(s) 449
MboI GATC 1 cut(s) 447
MboII GAAGA 1 cut(s) 154
MflI RGATCY 1 cut(s) 447
MhlI GDGCHC 2 cut(s) 45, 436
MluCI AATT 1 cut(s) 36
MlyI GAGTC 3 cut(s) 194, 257, 368
MnlI CCTC 6 cut(s) 99, 132, 200, 222, 229, 234
MroXI GAANNNNTTC 1 cut(s) 20
MseI TTAA 1 cut(s) 35
MspI CCGG 2 cut(s) 293, 455
MspR9I CCNGG 3 cut(s) 293, 294, 456
NciI CCSGG 3 cut(s) 293, 294, 456
NdeII GATC 1 cut(s) 447
NlaIII CATG 3 cut(s) 16, 263, 329
NlaIV GGNNCC 1 cut(s) 433
PaeR7I CTCGAG 1 cut(s) 44
PdmI GAANNNNTTC 1 cut(s) 20
PfeI GAWTC 1 cut(s) 316
PkrI GCNGC 1 cut(s) 331
PleI GAGTC 3 cut(s) 193, 257, 368
PpsI GAGTC 3 cut(s) 193, 257, 368
Psp124BI GAGCTC 1 cut(s) 45
PspEI GGTNACC 1 cut(s) 475
PspN4I GGNNCC 1 cut(s) 433
PspPI GGNCC 1 cut(s) 209
PsuI RGATCY 1 cut(s) 447
RsaI GTAC 1 cut(s) 277
RsaNI GTAC 1 cut(s) 276
SacI GAGCTC 1 cut(s) 45
SaqAI TTAA 1 cut(s) 35
SatI GCNGC 1 cut(s) 330
Sau3AI GATC 1 cut(s) 447
Sau96I GGNCC 1 cut(s) 209
SchI GAGTC 3 cut(s) 194, 257, 368
ScrFI CCNGG 3 cut(s) 293, 294, 456
SduI GDGCHC 2 cut(s) 45, 436
SfaNI GCATC 1 cut(s) 70
Sfr274I CTCGAG 1 cut(s) 44
SlaI CTCGAG 1 cut(s) 44
SmaI CCCGGG 1 cut(s) 294
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
Sse9I AATT 1 cut(s) 36
SspMI CTAG 3 cut(s) 71, 165, 378
SstI GAGCTC 1 cut(s) 45
StyD4I CCNGG 3 cut(s) 291, 292, 454
StyI CCWWGG 2 cut(s) 471, 494
TaaI ACNGT 1 cut(s) 275
TaqI TCGA 3 cut(s) 45, 76, 183
TasI AATT 1 cut(s) 36
TfiI GAWTC 1 cut(s) 316
Tru1I TTAA 1 cut(s) 35
Tru9I TTAA 1 cut(s) 35
TseI GCWGC 1 cut(s) 329
TspDTI ATGAA 3 cut(s) 29, 209, 314
TspMI CCCGGG 1 cut(s) 292
XbaI TCTAGA 1 cut(s) 377
XhoI CTCGAG 1 cut(s) 44
XmaI CCCGGG 1 cut(s) 292
XmnI GAANNNNTTC 1 cut(s) 20
XspI CTAG 3 cut(s) 71, 165, 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.