MD08G1181300.v1.1

rRNA-processing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0003163
Physical Location & Seq
Forward (+)
22489702 .. 22490486
785 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1181300.v1.1.491

Sequence Viewer

Length: 714 bp
ATGAGAGTGAAAAGGCAGAAGCGTCATCGGAAGAGTGTGAGGTTCTACACAGCATGCTACGGCTTCAGACAGCCCTACAGGGTGCTATGCGATGTTACTTTCGTGCATCACCTCATCACCAATCTCATTACCCCTGCTGACAAGGCCATCTCTAATGTCCTTGGTGCCCCTGTCCTGCTCTTCACCACCAAATGCGCCATCGCCAAGTTGAAACAACATGGTCCAATGCTTGGTCCTTCGTATTCACAGTCTCTTGAAGCTGCTAATAGCCTTATTACTGCAAGGTGTGATCACGAGAGTCACGTGAGTGCTGAAGATTGTATATTGGATGTTGTTGGGCAAAGTAATTCCGAGCACTTCTTTGTTGCTACTCATCACGTTGATCTTCATCGGAAACTTCTCAAGACACCAGGTGTCCCTGCTATTTATGCTCTGAGGACTGCTCTTTTGTTTAAGTCCCCTTCTGATAACCAACACCAATTTGTCAAAACTTTTAAAGAACAACGCTTGCATATGACTAACTTGGAATATAAGTTGTTGAAGAAGCAAAAGAATATTTCGAATTCTCTTAAAATAAATGATCCTCCTGGTGAAGAAGATGGTTATGGAGATCAGAACATGGAAGCGCAGGCTGTAGCAAATAGGGATATTGCTAGGATAGGATTGGGCGTGAAGGATAAAGTTCAATTTAATAGAAAGAAGGCAAAGCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fcf1

Protein Analysis

238

Amino Acids

26.79

Weight (kDa)

9.71

Isoelectric Point (pI)

32.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fcf1 PF04900 53 - 149 8e-21 Fcf1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 164
AccB7I CCANNNNNTGG 1 cut(s) 230
AclWI GGATC 1 cut(s) 575
AcsI RAATTY 1 cut(s) 562
AcuI CTGAAG 2 cut(s) 49, 333
AcvI CACGTG 1 cut(s) 304
AdeI CACNNNGTG 1 cut(s) 413
AfiI CCNNNNNNNGG 1 cut(s) 230
AgsI TTSAA 4 cut(s) 211, 257, 541, 686
AjnI CCWGG 2 cut(s) 409, 586
AleI CACNNNNGTG 1 cut(s) 306
AluBI AGCT 1 cut(s) 260
AluI AGCT 1 cut(s) 260
Alw21I GWGCWC 1 cut(s) 357
Alw26I GTCTC 1 cut(s) 255
AlwI GGATC 1 cut(s) 575
AoxI GGCC 1 cut(s) 144
ApeKI GCWGC 1 cut(s) 260
ApoI RAATTY 1 cut(s) 562
ArsI GACNNNNNNTTYG 2 cut(s) 430, 462
AspLEI GCGC 2 cut(s) 197, 628
AspS9I GGNCC 2 cut(s) 221, 233
AsuHPI GGTGA 4 cut(s) 101, 109, 175, 602
AsuII TTCGAA 1 cut(s) 560
AvaII GGWCC 2 cut(s) 221, 233
BaeGI GKGCMC 1 cut(s) 169
BanI GGYRCC 1 cut(s) 164
BauI CACGAG 1 cut(s) 293
BbrPI CACGTG 1 cut(s) 304
Bbv12I GWGCWC 1 cut(s) 357
BbvI GCAGC 1 cut(s) 247
BccI CCATC 3 cut(s) 155, 206, 593
BceAI ACGGC 1 cut(s) 76
BciT130I CCWGG 2 cut(s) 411, 588
BclI TGATCA 1 cut(s) 289
BcoDI GTCTC 1 cut(s) 255
BfaI CTAG 2 cut(s) 654, 712
BfmI CTRYAG 2 cut(s) 76, 633
BisI GCNGC 1 cut(s) 261
BlsI GCNGC 1 cut(s) 262
Bme1390I CCNGG 2 cut(s) 411, 588
Bme18I GGWCC 2 cut(s) 221, 233
BmgT120I GGNCC 2 cut(s) 221, 233
BmiI GGNNCC 1 cut(s) 166
BmrFI CCNGG 2 cut(s) 411, 588
BmsI GCATC 1 cut(s) 115
BplI GAGNNNNNCTC 2 cut(s) 427, 459
Bpu14I TTCGAA 1 cut(s) 560
BpuEI CTTGAG 1 cut(s) 386
BsaAI YACGTR 1 cut(s) 304
BsaBI GATNNNNATC 1 cut(s) 387
BsaJI CCNNGG 1 cut(s) 160
Bsc4I CCNNNNNNNGG 1 cut(s) 230
Bse8I GATNNNNATC 1 cut(s) 387
BseBI CCWGG 2 cut(s) 411, 588
BseDI CCNNGG 1 cut(s) 160
BseGI GGATG 1 cut(s) 334
BseJI GATNNNNATC 1 cut(s) 387
BseLI CCNNNNNNNGG 1 cut(s) 230
BseMII CTCAG 1 cut(s) 425
BseSI GKGCMC 1 cut(s) 169
BseXI GCAGC 1 cut(s) 247
BshFI GGCC 1 cut(s) 146
BshNI GGYRCC 1 cut(s) 164
BsiHKAI GWGCWC 1 cut(s) 357
BslFI GGGAC 2 cut(s) 401, 442
BslI CCNNNNNNNGG 1 cut(s) 230
BsmAI GTCTC 1 cut(s) 255
BsmFI GGGAC 2 cut(s) 401, 442
BsnI GGCC 1 cut(s) 146
Bsp119I TTCGAA 1 cut(s) 560
Bsp1286I GDGCHC 2 cut(s) 169, 357
Bsp143I GATC 4 cut(s) 289, 382, 580, 610
BspANI GGCC 1 cut(s) 146
BspCNI CTCAG 1 cut(s) 426
BspLI GGNNCC 1 cut(s) 166
BspPI GGATC 1 cut(s) 575
BspQI GCTCTTC 1 cut(s) 185
BspT104I TTCGAA 1 cut(s) 560
BspT107I GGYRCC 1 cut(s) 164
BssECI CCNNGG 1 cut(s) 160
BssMI GATC 4 cut(s) 289, 382, 580, 610
BssSI CACGAG 1 cut(s) 293
BssT1I CCWWGG 1 cut(s) 160
Bst2BI CACGAG 1 cut(s) 293
Bst2UI CCWGG 2 cut(s) 411, 588
Bst4CI ACNGT 1 cut(s) 249
Bst6I CTCTTC 2 cut(s) 26, 185
BstBAI YACGTR 1 cut(s) 304
BstBI TTCGAA 1 cut(s) 560
BstC8I GCNNGC 3 cut(s) 55, 509, 630
BstDEI CTNAG 1 cut(s) 434
BstF5I GGATG 1 cut(s) 334
BstHHI GCGC 2 cut(s) 197, 628
BstKTI GATC 4 cut(s) 292, 385, 583, 613
BstMAI GTCTC 1 cut(s) 255
BstMBI GATC 4 cut(s) 289, 382, 580, 610
BstMWI GCNNNNNNNGC 2 cut(s) 143, 428
BstNI CCWGG 2 cut(s) 411, 588
BstNSI RCATGY 1 cut(s) 57
BstSCI CCNGG 2 cut(s) 409, 586
BstSFI CTRYAG 2 cut(s) 76, 633
BstSLI GKGCMC 1 cut(s) 169
BstV1I GCAGC 1 cut(s) 247
BsuRI GGCC 1 cut(s) 146
BtgZI GCGATG 2 cut(s) 105, 184
BtsCI GGATG 1 cut(s) 334
Cac8I GCNNGC 3 cut(s) 55, 509, 630
CfoI GCGC 2 cut(s) 197, 628
Cfr13I GGNCC 2 cut(s) 221, 233
CseI GACGC 1 cut(s) 11
CsiI ACCWGGT 1 cut(s) 409
CviAII CATG 3 cut(s) 54, 218, 619
CviJI RGCY 7 cut(s) 63, 73, 146, 260, 270, 632, 709
CviKI_1 RGCY 7 cut(s) 63, 73, 146, 260, 270, 632, 709
DdeI CTNAG 1 cut(s) 434
DpnI GATC 4 cut(s) 291, 384, 582, 612
DpnII GATC 4 cut(s) 289, 382, 580, 610
DraI TTTAAA 1 cut(s) 496
DraIII CACNNNGTG 1 cut(s) 413
Eam1104I CTCTTC 2 cut(s) 26, 185
EarI CTCTTC 2 cut(s) 26, 185
Eco130I CCWWGG 1 cut(s) 160
Eco47I GGWCC 2 cut(s) 221, 233
Eco57I CTGAAG 2 cut(s) 49, 333
Eco72I CACGTG 1 cut(s) 304
EcoRI GAATTC 1 cut(s) 562
EcoRII CCWGG 2 cut(s) 409, 586
EcoT14I CCWWGG 1 cut(s) 160
ErhI CCWWGG 1 cut(s) 160
FaeI CATG 3 cut(s) 57, 221, 622
FaqI GGGAC 2 cut(s) 401, 442
FatI CATG 3 cut(s) 53, 217, 618
FauNDI CATATG 1 cut(s) 513
FbaI TGATCA 1 cut(s) 289
Fnu4HI GCNGC 1 cut(s) 261
FokI GGATG 1 cut(s) 341
Fsp4HI GCNGC 1 cut(s) 261
FspBI CTAG 2 cut(s) 654, 712
GlaI GCGC 2 cut(s) 196, 627
GluI GCNGC 1 cut(s) 261
HaeIII GGCC 1 cut(s) 146
HgaI GACGC 1 cut(s) 11
HhaI GCGC 2 cut(s) 197, 628
Hin1II CATG 3 cut(s) 57, 221, 622
Hin6I GCGC 2 cut(s) 195, 626
HinP1I GCGC 2 cut(s) 195, 626
HinfI GANTC 1 cut(s) 298
HphI GGTGA 4 cut(s) 101, 109, 175, 602
Hpy188I TCNGA 7 cut(s) 30, 68, 352, 393, 435, 466, 615
Hpy188III TCNNGA 3 cut(s) 254, 293, 403
HpyAV CCTTC 4 cut(s) 246, 471, 667, 694
HpyCH4III ACNGT 1 cut(s) 249
HpyCH4IV ACGT 2 cut(s) 303, 378
HpyCH4V TGCA 3 cut(s) 106, 281, 511
HpyF10VI GCNNNNNNNGC 2 cut(s) 143, 428
HpyF3I CTNAG 1 cut(s) 434
HpySE526I ACGT 2 cut(s) 303, 378
Hsp92II CATG 3 cut(s) 57, 221, 622
HspAI GCGC 2 cut(s) 195, 626
Ksp22I TGATCA 1 cut(s) 289
Kzo9I GATC 4 cut(s) 289, 382, 580, 610
LguI GCTCTTC 1 cut(s) 185
Lsp1109I GCAGC 1 cut(s) 247
LweI GCATC 1 cut(s) 115
MabI ACCWGGT 1 cut(s) 409
MaeI CTAG 2 cut(s) 654, 712
MaeII ACGT 2 cut(s) 303, 378
MaeIII GTNAC 2 cut(s) 94, 299
MalI GATC 4 cut(s) 291, 384, 582, 612
MboI GATC 4 cut(s) 289, 382, 580, 610
MboII GAAGA 7 cut(s) 43, 172, 326, 377, 553, 605, 608
MhlI GDGCHC 2 cut(s) 169, 357
MluCI AATT 4 cut(s) 346, 479, 562, 686
MlyI GAGTC 1 cut(s) 307
MnlI CCTC 4 cut(s) 33, 122, 429, 594
MseI TTAA 4 cut(s) 453, 495, 570, 690
MslI CAYNNNNRTG 1 cut(s) 306
MspR9I CCNGG 2 cut(s) 411, 588
MvaI CCWGG 2 cut(s) 411, 588
MwoI GCNNNNNNNGC 2 cut(s) 143, 428
NdeI CATATG 1 cut(s) 513
NdeII GATC 4 cut(s) 289, 382, 580, 610
NlaIII CATG 3 cut(s) 57, 221, 622
NlaIV GGNNCC 1 cut(s) 166
NmuCI GTSAC 1 cut(s) 299
NspI RCATGY 1 cut(s) 57
NspV TTCGAA 1 cut(s) 560
OliI CACNNNNGTG 1 cut(s) 306
PaeI GCATGC 1 cut(s) 57
PciSI GCTCTTC 1 cut(s) 185
PcsI WCGNNNNNNNCGW 1 cut(s) 300
PflMI CCANNNNNTGG 1 cut(s) 230
PkrI GCNGC 1 cut(s) 262
PleI GAGTC 1 cut(s) 306
PmaCI CACGTG 1 cut(s) 304
PmlI CACGTG 1 cut(s) 304
PpsI GAGTC 1 cut(s) 306
Ppu21I YACGTR 1 cut(s) 304
Psp6I CCWGG 2 cut(s) 409, 586
PspCI CACGTG 1 cut(s) 304
PspGI CCWGG 2 cut(s) 409, 586
PspN4I GGNNCC 1 cut(s) 166
PspPI GGNCC 2 cut(s) 221, 233
RseI CAYNNNNRTG 1 cut(s) 306
SapI GCTCTTC 1 cut(s) 185
SaqAI TTAA 4 cut(s) 453, 495, 570, 690
SatI GCNGC 1 cut(s) 261
Sau3AI GATC 4 cut(s) 289, 382, 580, 610
Sau96I GGNCC 2 cut(s) 221, 233
SchI GAGTC 1 cut(s) 307
ScrFI CCNGG 2 cut(s) 411, 588
SduI GDGCHC 2 cut(s) 169, 357
SetI ASST 7 cut(s) 44, 114, 262, 287, 306, 381, 415
SexAI ACCWGGT 1 cut(s) 409
SfaNI GCATC 1 cut(s) 115
SfcI CTRYAG 2 cut(s) 76, 633
SfuI TTCGAA 1 cut(s) 560
SinI GGWCC 2 cut(s) 221, 233
SmiMI CAYNNNNRTG 1 cut(s) 306
SmlI CTYRAG 1 cut(s) 401
SmoI CTYRAG 1 cut(s) 401
SphI GCATGC 1 cut(s) 57
Sse9I AATT 4 cut(s) 346, 479, 562, 686
SspI AATATT 1 cut(s) 556
SspMI CTAG 2 cut(s) 654, 712
StyD4I CCNGG 2 cut(s) 409, 586
StyI CCWWGG 1 cut(s) 160
TaaI ACNGT 1 cut(s) 249
TaiI ACGT 2 cut(s) 306, 381
TaqI TCGA 1 cut(s) 560
TasI AATT 4 cut(s) 346, 479, 562, 686
Tru1I TTAA 4 cut(s) 453, 495, 570, 690
Tru9I TTAA 4 cut(s) 453, 495, 570, 690
TseFI GTSAC 1 cut(s) 299
TseI GCWGC 1 cut(s) 260
Tsp45I GTSAC 1 cut(s) 299
TspDTI ATGAA 1 cut(s) 377
Van91I CCANNNNNTGG 1 cut(s) 230
VpaK11BI GGWCC 2 cut(s) 221, 233
XapI RAATTY 1 cut(s) 562
XceI RCATGY 1 cut(s) 57
XspI CTAG 2 cut(s) 654, 712
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.