MD16G1246600.v1.1

rRNA-processing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0003163
Physical Location & Seq
Reverse (-)
27285539 .. 27286132
594 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1246600.v1.1.491

Sequence Viewer

Length: 594 bp
ATGATAGTGAAAAGGCAGAAGCGTCACCGAAAGAGTGTGAGGTTCTACACAGCATGCTACGGCTTCAAACAGCCCTACAAGGTGTTGTGCAATGTTACTTTCGTGCATCACCTCGTCACCAATCGCATTACCCCCGCTGACAAGGCCATCTCTAATGTCCTCGGTGCCCCTGTCCTGCTCTTCACCACCAAATGCGCCATCGCCGAGTTGAAACAACATGGTCCGATGTTTGATCATTCGTATTCACAGTCTCTTGAAACTGCTAATAGCCTTATTACCGCAAGGTGTGATCACGAGAAACACGTGAGTGCTGAAGATTATATATTGGATGTTGTTGGGCAAAGTAATTCCGAGCACTTCTTTGTTGCTACTCAGCACGTTGATCTTCGTCGGAAACTTCTCAAGATGCCAGATGTCCATGTTATTTATGCTCTGAGGACTGCTCTTTTGCTTGAGTCCCCTTCGGATGACCAACACCAATTTGTCAAAACTTCTGAAGAACAACGCTTGCATATGACTGACTTGGAATATAAGTTGTTGAAGAAGCAAAAGAATATTCTGAATTCTCTTAAAAATAAACGATCCTTCTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fcf1

Protein Analysis

198

Amino Acids

22.86

Weight (kDa)

9.49

Isoelectric Point (pI)

50.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fcf1 PF04900 53 - 152 5.9e-20 Fcf1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 164
AciI CCGC 2 cut(s) 135, 279
AclWI GGATC 1 cut(s) 576
AcsI RAATTY 1 cut(s) 562
AcuI CTGAAG 2 cut(s) 333, 516
AcvI CACGTG 1 cut(s) 304
AflIII ACRYGT 1 cut(s) 301
AgsI TTSAA 4 cut(s) 67, 211, 257, 541
AleI CACNNNNGTG 1 cut(s) 306
Alw21I GWGCWC 1 cut(s) 357
Alw26I GTCTC 1 cut(s) 255
AlwI GGATC 1 cut(s) 576
AoxI GGCC 1 cut(s) 144
ApoI RAATTY 1 cut(s) 562
ArsI GACNNNNNNTTYG 2 cut(s) 430, 462
AspLEI GCGC 1 cut(s) 197
AspS9I GGNCC 1 cut(s) 221
AsuHPI GGTGA 4 cut(s) 17, 101, 109, 175
AvaII GGWCC 1 cut(s) 221
BaeGI GKGCMC 1 cut(s) 169
BanI GGYRCC 1 cut(s) 164
BauI CACGAG 1 cut(s) 293
BbrPI CACGTG 1 cut(s) 304
Bbv12I GWGCWC 1 cut(s) 357
BccI CCATC 2 cut(s) 155, 206
BceAI ACGGC 1 cut(s) 76
BclI TGATCA 2 cut(s) 232, 289
BcoDI GTCTC 1 cut(s) 255
Bme18I GGWCC 1 cut(s) 221
BmgT120I GGNCC 1 cut(s) 221
BmiI GGNNCC 1 cut(s) 166
BmsI GCATC 2 cut(s) 115, 396
BplI GAGNNNNNCTC 2 cut(s) 427, 459
BpuEI CTTGAG 2 cut(s) 386, 473
BsaAI YACGTR 1 cut(s) 304
BsaJI CCNNGG 1 cut(s) 160
Bse3DI GCAATG 1 cut(s) 97
BseDI CCNNGG 1 cut(s) 160
BseGI GGATG 2 cut(s) 334, 472
BseMI GCAATG 1 cut(s) 97
BseMII CTCAG 2 cut(s) 386, 425
BseSI GKGCMC 1 cut(s) 169
BshFI GGCC 1 cut(s) 146
BshNI GGYRCC 1 cut(s) 164
BsiHKAI GWGCWC 1 cut(s) 357
BslFI GGGAC 1 cut(s) 442
BsmAI GTCTC 1 cut(s) 255
BsmFI GGGAC 1 cut(s) 442
BsnI GGCC 1 cut(s) 146
Bsp1286I GDGCHC 2 cut(s) 169, 357
Bsp143I GATC 4 cut(s) 232, 289, 382, 581
BspACI CCGC 2 cut(s) 135, 279
BspANI GGCC 1 cut(s) 146
BspCNI CTCAG 2 cut(s) 385, 426
BspLI GGNNCC 1 cut(s) 166
BspPI GGATC 1 cut(s) 576
BspQI GCTCTTC 1 cut(s) 185
BspT107I GGYRCC 1 cut(s) 164
BsrDI GCAATG 1 cut(s) 97
BssECI CCNNGG 1 cut(s) 160
BssMI GATC 4 cut(s) 232, 289, 382, 581
BssSI CACGAG 1 cut(s) 293
Bst2BI CACGAG 1 cut(s) 293
Bst4CI ACNGT 1 cut(s) 249
Bst6I CTCTTC 1 cut(s) 185
BstBAI YACGTR 1 cut(s) 304
BstC8I GCNNGC 2 cut(s) 55, 509
BstDEI CTNAG 2 cut(s) 372, 434
BstF5I GGATG 2 cut(s) 334, 472
BstHHI GCGC 1 cut(s) 197
BstKTI GATC 4 cut(s) 235, 292, 385, 584
BstMAI GTCTC 1 cut(s) 255
BstMBI GATC 4 cut(s) 232, 289, 382, 581
BstMWI GCNNNNNNNGC 1 cut(s) 143
BstNSI RCATGY 1 cut(s) 57
BstSLI GKGCMC 1 cut(s) 169
BsuRI GGCC 1 cut(s) 146
BtgZI GCGATG 1 cut(s) 184
BtsCI GGATG 2 cut(s) 334, 472
Cac8I GCNNGC 2 cut(s) 55, 509
CfoI GCGC 1 cut(s) 197
Cfr13I GGNCC 1 cut(s) 221
CseI GACGC 1 cut(s) 11
CviAII CATG 3 cut(s) 54, 218, 419
CviJI RGCY 4 cut(s) 63, 73, 146, 270
CviKI_1 RGCY 4 cut(s) 63, 73, 146, 270
DdeI CTNAG 2 cut(s) 372, 434
DpnI GATC 4 cut(s) 234, 291, 384, 583
DpnII GATC 4 cut(s) 232, 289, 382, 581
Eam1104I CTCTTC 1 cut(s) 185
EarI CTCTTC 1 cut(s) 185
Eco47I GGWCC 1 cut(s) 221
Eco57I CTGAAG 2 cut(s) 333, 516
Eco72I CACGTG 1 cut(s) 304
EcoRI GAATTC 1 cut(s) 562
FaeI CATG 3 cut(s) 57, 221, 422
FaiI YATR 9 cut(s) 55, 219, 321, 323, 420, 429, 513, 515, 531
FaqI GGGAC 1 cut(s) 442
FatI CATG 3 cut(s) 53, 217, 418
FauI CCCGC 1 cut(s) 142
FauNDI CATATG 1 cut(s) 513
FbaI TGATCA 2 cut(s) 232, 289
FokI GGATG 2 cut(s) 341, 479
GlaI GCGC 1 cut(s) 196
HaeIII GGCC 1 cut(s) 146
HgaI GACGC 1 cut(s) 11
HhaI GCGC 1 cut(s) 197
Hin1II CATG 3 cut(s) 57, 221, 422
Hin6I GCGC 1 cut(s) 195
HinP1I GCGC 1 cut(s) 195
HinfI GANTC 1 cut(s) 455
HphI GGTGA 4 cut(s) 17, 101, 109, 175
Hpy188I TCNGA 7 cut(s) 225, 352, 393, 435, 466, 496, 561
Hpy188III TCNNGA 3 cut(s) 254, 293, 403
Hpy99I CGWCG 1 cut(s) 393
HpyAV CCTTC 1 cut(s) 471
HpyCH4III ACNGT 1 cut(s) 249
HpyCH4IV ACGT 2 cut(s) 303, 378
HpyCH4V TGCA 3 cut(s) 90, 106, 511
HpyF10VI GCNNNNNNNGC 1 cut(s) 143
HpyF3I CTNAG 2 cut(s) 372, 434
HpySE526I ACGT 2 cut(s) 303, 378
Hsp92II CATG 3 cut(s) 57, 221, 422
HspAI GCGC 1 cut(s) 195
Ksp22I TGATCA 2 cut(s) 232, 289
Kzo9I GATC 4 cut(s) 232, 289, 382, 581
LguI GCTCTTC 1 cut(s) 185
LpnPI CCDG 4 cut(s) 183, 188, 423, 574
LweI GCATC 2 cut(s) 115, 396
MaeII ACGT 2 cut(s) 303, 378
MaeIII GTNAC 3 cut(s) 23, 94, 115
MalI GATC 4 cut(s) 234, 291, 384, 583
MboI GATC 4 cut(s) 232, 289, 382, 581
MboII GAAGA 5 cut(s) 172, 326, 377, 509, 553
MhlI GDGCHC 2 cut(s) 169, 357
MluCI AATT 3 cut(s) 346, 479, 562
MlyI GAGTC 1 cut(s) 464
MmeI TCCRAC 1 cut(s) 371
MnlI CCTC 4 cut(s) 33, 122, 170, 429
MseI TTAA 1 cut(s) 570
MslI CAYNNNNRTG 1 cut(s) 306
MspA1I CMGCKG 1 cut(s) 137
MwoI GCNNNNNNNGC 1 cut(s) 143
NdeI CATATG 1 cut(s) 513
NdeII GATC 4 cut(s) 232, 289, 382, 581
NlaIII CATG 3 cut(s) 57, 221, 422
NlaIV GGNNCC 1 cut(s) 166
NmeAIII GCCGAG 1 cut(s) 229
NmuCI GTSAC 2 cut(s) 23, 115
NspI RCATGY 1 cut(s) 57
OliI CACNNNNGTG 1 cut(s) 306
PaeI GCATGC 1 cut(s) 57
PciSI GCTCTTC 1 cut(s) 185
PcsI WCGNNNNNNNCGW 1 cut(s) 300
PleI GAGTC 1 cut(s) 463
PmaCI CACGTG 1 cut(s) 304
PmlI CACGTG 1 cut(s) 304
PpsI GAGTC 1 cut(s) 463
Ppu21I YACGTR 1 cut(s) 304
PspCI CACGTG 1 cut(s) 304
PspN4I GGNNCC 1 cut(s) 166
PspPI GGNCC 1 cut(s) 221
RseI CAYNNNNRTG 1 cut(s) 306
SapI GCTCTTC 1 cut(s) 185
SaqAI TTAA 1 cut(s) 570
Sau3AI GATC 4 cut(s) 232, 289, 382, 581
Sau96I GGNCC 1 cut(s) 221
SchI GAGTC 1 cut(s) 464
SduI GDGCHC 2 cut(s) 169, 357
SetI ASST 6 cut(s) 44, 84, 114, 287, 306, 381
SfaNI GCATC 2 cut(s) 115, 396
SinI GGWCC 1 cut(s) 221
SmiMI CAYNNNNRTG 1 cut(s) 306
SmlI CTYRAG 2 cut(s) 401, 452
SmoI CTYRAG 2 cut(s) 401, 452
SphI GCATGC 1 cut(s) 57
Sse9I AATT 3 cut(s) 346, 479, 562
SsiI CCGC 2 cut(s) 135, 279
SspI AATATT 1 cut(s) 556
TaaI ACNGT 1 cut(s) 249
TaiI ACGT 2 cut(s) 306, 381
TasI AATT 3 cut(s) 346, 479, 562
Tru1I TTAA 1 cut(s) 570
Tru9I TTAA 1 cut(s) 570
TseFI GTSAC 2 cut(s) 23, 115
Tsp45I GTSAC 2 cut(s) 23, 115
VpaK11BI GGWCC 1 cut(s) 221
XapI RAATTY 1 cut(s) 562
XceI RCATGY 1 cut(s) 57
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.