Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0002627

Physical Location & Seq

Genomic Coordinates Reverse (-)

25590725 .. 25603786

Gene Structure 13062 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1197400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3291 bp

ATGAAGACTAGCGGTCTCGAAGTCTTCGACTTCAGCGAGGAGGACGACCACTCGGAATCGCTCCAGGGGAAATATTTTGGCAAGTTAAAAAACCCTAGGCTCGATACGAATCCGAATTTCAAGTACGATTTTCTCCAGAATGTTGTGCAGGGAGCCAAACTTGAATCAAAAGATATTGGCAGCATACCTTGTGTAGATGTTGATTCAGTTGACCAAGATCATTGTTGTGATAATGCCATTTCATATTCCCCCGTGGGAACAATTGAAGAGAGCCTTGCTACCAAGATAGAGTATATGGAATTGGATGCTGCACCACAATTCAAATGTTTGAGTCATGAACAACACTCTGATTCCAAATTAGATAGTCATGGATCCAGAAGTCCTGTTTCTGAGCCAGAGAGAAGAGGTTCAAATGCTATGTCATCATCGTCATGGAAAAGCCAGTTACACTCTGCCCTTGAGGTGTCTCCGTCCAGTAATGACCCAGTTGATGTGATTTTAGATGCTGATGAAAGCTCGAGTGACTCTCCATCAAGTCCTGCTTCTGAAATTGAAGAGGATGACGATTCTTTGGGTACATATAAACCGTATCATTGCTCTGGTGATTTGGAAATGGATAATATAAATATGACGGTTGTTCTTTATCCTGATTATGTTATATATCGGGATAGTTATTGTACAGAACCTCAGTTAACCTTTTCTCCCGGTTGCATCAAAATCAGTGGTTCAACATCCGAACGCCCTGGAACCTTTAGTTTTGAATGGGAAGTCGGTGATCTCATTGATGTAGAGTGTCAGTGGTTTCAAAAAGTTGAATTTGTCATGATAAAACTTCGTGTTGTATCAAAGAATGCCACGGAAGATGATGGTGCACCCAGTACTTCTGGCACTGAAGAGTTGAAGATTGCAAGTGTTGAACCCAACTGGTTCGAGCAACAACAAAGGATAATGTCTTTGAATGCAAAATATATCAATTCATGGGTTTTGCATGACATGGGCATGGAAACAGATGAGGACGATTCAATTGGGCAGAGGCATCATTTTCCGAATTTTGATGAGCCTTTTGAAGATGTTGTATATCCGAAAGGGGACTCAGATGCTGTTTCCATCAGCAAGAGAGATGTTGATCTCTTACAGCCAGAGACATTTATTAATGATACAATTATTGACTTCTATATCAAGTATCTGAAGAATCAGATTCAGCCTGAGAAAAGGCATAGGTTCCACTTTTTCAATAGTTTTTTCTTTCGGAAGCTGGCTGACCTGGACAAAGATCCATCCAGTGTTTCCGATGGCAGAGCTGCTTTTCAACGAGTTCGTAAATGGACGAGGAAAGTGGATTTATTTGAAAGGGATTACATCTTCATTCCTATAAACTTCAATCTACACTGGAGCCTAATAGTCATATGCCATCTTGGTGAAGTGCCTAAACATAATGATGGAGAATCAGGAAATTCACGTAAAGTACCTTGTATTTTACACATGGATTCTATCAAAGGAAGTCATACGGGTCTGAAAAATCTAATTCAAAGTTATCTGTGGGAAGAGTGGAAAGAAAGGAAAAAGGAGACATCTGAAGAAATCTCATCAAAGTTTCACAACCTGCGATTTGTCTCACTTGAGCTACCGCAACAGGAAAATTCGTTCGATTGTGGCCTGTTTTTACTCCACTATTTGGAGCTCTTTTTGGCAGAAGCTCCTGTTCATTTCAGCCCTTTCAAAATAACCAAGTTTTCCAACTTTCTTAATGCAAATTGGTTTCTTCCGTCCGAGGCATCTCTCAAGCGTACCCTTATTCAAAGGTTAATTTTTGAGCTACTTGAAGATCGTTGTCGGGGACTCTCTTCAGCAGCTTCCAGTGATGAAGACCAGGCTAAGTTTCCAGAATGTAACAAGCATGAAACTGGTGTGCAGTCTTTTTCAGGAAGATGTGGTCCTGCTGTAGCTTGCCAAGAAAATATGTCAAGTTCTCAAGCAGGCCAGGGAATTGAAATCACTCTATTATCCTCATCTTCTCTAAGGAGTTCTGAGTGTGTTAATGATGCAGGCTTGGTTCTCCAGGAACTTTTAGAGCCAGGAGCCACCCCCGGCTCACTCTATGGAGAATATCAATCATTTGACCAAAAATCATCTTTTTACCGTCTTAATGGAGCTATATCACCGATGGAGGACGACACCAAAACTGGAGAGCAGTTCGCTTTTATGCCTACAGGAGACTCTGGTTTTCAGCAAATAACTGGAATTACATCTCAAACTTGTGACATTGAGCATACATCAAGAGCTTATGGCGTTGAGACTGACTTTAACTTGGCGCAAGCGGAAAATGGAAACACTGATTCATCCCCTAAACTATCAATGTGTGTCTCTGACCAATCTGAATATATAGCGGTCATTGAGAACCATTCAGTTGGGGAAGGTTTGGGTTCGAGTCAGAAAGAAGAAATGGATGTAAACCATTCTCATTTTGTGGAAAACGTCACATGTTTAATAGATGTCCTTGTTTCTGCTCCAAGCAAGATGCAGGACGCTTCCATTATAGAATTAGAAGGTTCTCAAGATCATGTGCATGACTGGAATGAAAATGGAGATTCTCAAGACCATGATGCTTTCCATGATGGCAACGGAAGTTGTCAAGACCATTATAAGGTGCATGATGGCAACAAAAATGGAGGTTCTGAAGACCATGGTAAGGTGCGTGATGGCAAAAATGGAGGTTCCCAAGACCATGATATAGTGCTGGATGCCAATGAAAATAGAGGTTCTCAAGACCATGATAGATTGCATCATGGCAACAAAACTACAGGTTTTCAAGACCATGATAAGGTGCATGCTGTCAACGTAAATGGAGCTTCTCAAGACCATGATAAGGTGCAGGATGGCAACGAAATTGGAGGCTCTCATGACCATGATAAGGTGCTTGATGGCTACGAAAATGGAGCTTCTGAAGACCATGATGAGGTGCAGGATGGCAACGAAAATGGAGGCTTTCAGGACCATGATAAGGTGCAAGATGGCAACAGAAATGGAGCTTCTGAACACCATGGTATGGTGCAGGAAGCGGCTCCAATTCCCTCGTGCCAAGAAAATCCCGATGTACAAATGGATCAAGACTCCGATATGGTAGACAACAGGACAGTCTCATGTGATGATGATGTTCAAATTTCCGATGGACCAACGCCTGACGATGGTCTGATACCCGAGTCGCTAGAGCAACGCGCTGCAAAAAGGCTGCGGCTTACACCTCCAGTTGAAGGGGATAAATGTGTCACAAGAAGCCTTGTTGAAGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0006464 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009987 GO:0016787 GO:0016926 GO:0016929 GO:0018193 GO:0018205 GO:0019538 GO:0019783 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0070011 GO:0070137 GO:0070139 GO:0070646 GO:0070647 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_C48

Protein Analysis

1097

Amino Acids

122.37

Weight (kDa)

4.62

Isoelectric Point (pI)

48.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PH_ULP	PF25352	210 - 327	2.4e-45	Ubiquitin-like protease PH domain
Peptidase_C48	PF02902	383 - 577	1.4e-35	Ulp1 protease family, C-terminal catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002627)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09730 AT1G09730
fragaria_vesca	FvH4_3g25421 FvH4_5g35390 FvH4_5g35390 FvH4_5g35390 FvH4_5g35390
malus_domestica	MD08G1197400.v1.1 MD15G1385000.v1.1
prunus_persica	Prupe.6G149300_v2.0.a1 Prupe.6G149300_v2.0.a1 Prupe.6G149300_v2.0.a1 Prupe.6G149300_v2.0.a1 Prupe.6G149300_v2.0.a1 Prupe.6G149300_v2.0.a1 Prupe.6G149300_v2.0.a1 Prupe.6G149300_v2.0.a1 Prupe.6G149300_v2.0.a1 Prupe.6G149300_v2.0.a1 Prupe.6G149300_v2.0.a1 Prupe.6G149300_v2.0.a1 Prupe.6G149300_v2.0.a1
pyrus_communis	pycom08g16950 pycom08g16970 pycom15g34500
rosa_chinensis	RchiOBHm_Chr7g0236781
rosa_laevigata	RLG00000001062
rosa_multiflora	Rmu_sc0003720.1_g000028
rosa_roxburghii	Rroxscaffold_3G00224860
rosa_rugosa	Rorug07G0298800 Rorug07G0298900 Rorug07G0299000.1 Rorug07G0299100 Rorug07G0299200
rosa_samantha	Rh7AG454400 Rh7BG425600 Rh7CG472900 Rh7DG442100
rosa_wichuraiana	Rw7G037660 Rw7G037720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2643
Acc36I	ACCTGC	1 cut(s)	1611
AccB7I	CCANNNNNTGG	2 cut(s)	1675, 3046
AccI	GTMKAC	1 cut(s)	3123
AccII	CGCG	1 cut(s)	3216
AciI	CCGC	6 cut(s)	12, 1628, 2318, 2387, 3059, 3232
AclWI	GGATC	4 cut(s)	366, 379, 1268, 3111
AcsI	RAATTY	6 cut(s)	115, 815, 1048, 1453, 1639, 3159
AcuI	CTGAAG	7 cut(s)	16, 912, 1208, 1596, 1830, 2697, 2964
AfaI	GTAC	7 cut(s)	125, 577, 679, 880, 1467, 1789, 3096
AflIII	ACRYGT	1 cut(s)	2480
AhdI	GACNNNNNGTC	1 cut(s)	12
AjnI	CCWGG	7 cut(s)	63, 742, 1263, 1869, 1980, 2058, 2074
AloI	GAACNNNNNNTCC	2 cut(s)	1628, 1660
Alw21I	GWGCWC	2 cut(s)	874, 1683
Alw26I	GTCTC	9 cut(s)	20, 471, 1136, 1562, 1618, 2208, 2288, 2368, 3142
Alw44I	GTGCAC	1 cut(s)	870
AlwI	GGATC	4 cut(s)	366, 379, 1268, 3111
AlwNI	CAGNNNCTG	1 cut(s)	1100
Ama87I	CYCGRG	2 cut(s)	517, 3197
AoxI	GGCC	2 cut(s)	1654, 1978
ApaLI	GTGCAC	1 cut(s)	870
ApeKI	GCWGC	6 cut(s)	180, 308, 1301, 1850, 3218, 3229
ApoI	RAATTY	6 cut(s)	115, 815, 1048, 1453, 1639, 3159
ArsI	GACNNNNNNTTYG	2 cut(s)	404, 436
AseI	ATTAAT	1 cut(s)	1152
Asp700I	GAANNNNTTC	1 cut(s)	406
AspA2I	CCTAGG	1 cut(s)	95
AspLEI	GCGC	2 cut(s)	2314, 3218
AspS9I	GGNCC	3 cut(s)	1934, 2992, 3170
AsuC2I	CCSGG	2 cut(s)	705, 2088
AsuHPI	GGTGA	4 cut(s)	614, 785, 1430, 2151
AvaI	CYCGRG	2 cut(s)	517, 3197
AvaII	GGWCC	3 cut(s)	1934, 2992, 3170
AvrII	CCTAGG	1 cut(s)	95
BaeGI	GKGCMC	1 cut(s)	874
BaeI	ACNNNNGTAYC	2 cut(s)	3185, 3218
BamHI	GGATCC	1 cut(s)	371
BanII	GRGCYC	1 cut(s)	1683
BauI	CACGAG	1 cut(s)	3073
BbsI	GAAGAC	5 cut(s)	11, 16, 1872, 2685, 2952
Bbv12I	GWGCWC	2 cut(s)	874, 1683
BbvI	GCAGC	6 cut(s)	192, 295, 1288, 1862, 3205, 3216
BciT130I	CCWGG	7 cut(s)	65, 744, 1265, 1871, 1982, 2060, 2076
BcnI	CCSGG	2 cut(s)	705, 2088
BcoDI	GTCTC	9 cut(s)	20, 471, 1136, 1562, 1618, 2208, 2288, 2368, 3142
BfaI	CTAG	3 cut(s)	9, 96, 3206
BfmI	CTRYAG	3 cut(s)	1941, 2208, 2799
BfuAI	ACCTGC	1 cut(s)	1611
BisI	GCNGC	8 cut(s)	181, 309, 1302, 1851, 3060, 3219, 3230, 3233
BlnI	CCTAGG	1 cut(s)	95
BlsI	GCNGC	8 cut(s)	182, 310, 1303, 1852, 3061, 3220, 3231, 3234
BmcAI	AGTACT	1 cut(s)	880
Bme1390I	CCNGG	9 cut(s)	65, 705, 744, 1265, 1871, 1982, 2060, 2076, 2088
Bme18I	GGWCC	3 cut(s)	1934, 2992, 3170
BmeRI	GACNNNNNGTC	1 cut(s)	12
BmeT110I	CYCGRG	2 cut(s)	517, 3197
BmgT120I	GGNCC	3 cut(s)	1934, 2992, 3170
BmiI	GGNNCC	8 cut(s)	154, 373, 748, 1223, 1394, 2080, 2716, 3063
BmrFI	CCNGG	9 cut(s)	65, 705, 744, 1265, 1871, 1982, 2060, 2076, 2088
BmrI	ACTGGG	2 cut(s)	479, 870
BmuI	ACTGGG	2 cut(s)	479, 870
BoxI	GACNNNNGTC	1 cut(s)	3186
BpiI	GAAGAC	5 cut(s)	11, 16, 1872, 2685, 2952
BplI	GAGNNNNNCTC	2 cut(s)	511, 543
BpmI	CTGGAG	6 cut(s)	47, 119, 1411, 2042, 2205, 3228
BpuEI	CTTGAG	8 cut(s)	479, 1640, 1766, 1956, 2538, 2577, 2748, 2838
BpuMI	CCSGG	2 cut(s)	705, 2088
BsaAI	YACGTR	1 cut(s)	1460
BsaBI	GATNNNNATC	2 cut(s)	108, 1125
BsaI	GGTCTC	1 cut(s)	20
BsaXI	ACNNNNNCTCC	2 cut(s)	685, 715
Bse3DI	GCAATG	1 cut(s)	592
Bse8I	GATNNNNATC	2 cut(s)	108, 1125
BseBI	CCWGG	7 cut(s)	65, 744, 1265, 1871, 1982, 2060, 2076
BseGI	GGATG	9 cut(s)	310, 565, 731, 1277, 2339, 2452, 2746, 2881, 2971
BseJI	GATNNNNATC	2 cut(s)	108, 1125
BseMI	GCAATG	1 cut(s)	592
BseMII	CTCAG	5 cut(s)	381, 701, 1107, 1197, 2019
BseRI	GAGGAG	1 cut(s)	53
BseSI	GKGCMC	1 cut(s)	874
BseXI	GCAGC	6 cut(s)	192, 295, 1288, 1862, 3205, 3216
BsgI	GTGCAG	6 cut(s)	167, 294, 1931, 2891, 2981, 3071
Bsh1236I	CGCG	1 cut(s)	3216
BshFI	GGCC	2 cut(s)	1656, 1980
BsiHKAI	GWGCWC	2 cut(s)	874, 1683
BsiHKCI	CYCGRG	2 cut(s)	517, 3197
BsiSI	CCGG	2 cut(s)	705, 2088
BslFI	GGGAC	2 cut(s)	1103, 1851
BsmAI	GTCTC	9 cut(s)	20, 471, 1136, 1562, 1618, 2208, 2288, 2368, 3142
BsmFI	GGGAC	2 cut(s)	1103, 1851
BsmI	GAATGC	2 cut(s)	856, 964
BsnI	GGCC	2 cut(s)	1656, 1980
Bso31I	GGTCTC	1 cut(s)	20
BsoBI	CYCGRG	2 cut(s)	517, 3197
Bsp1286I	GDGCHC	2 cut(s)	874, 1683
Bsp1407I	TGTACA	2 cut(s)	677, 3094
Bsp143I	GATC	8 cut(s)	217, 371, 775, 1126, 1273, 1825, 2557, 3103
Bsp19I	CCATGG	2 cut(s)	2683, 3040
BspACI	CCGC	6 cut(s)	12, 1628, 2318, 2387, 3059, 3232
BspANI	GGCC	2 cut(s)	1656, 1980
BspCNI	CTCAG	5 cut(s)	382, 700, 1106, 1198, 2020
BspFNI	CGCG	1 cut(s)	3216
BspHI	TCATGA	3 cut(s)	334, 822, 2899
BspLI	GGNNCC	8 cut(s)	154, 373, 748, 1223, 1394, 2080, 2716, 3063
BspMI	ACCTGC	1 cut(s)	1611
BspPI	GGATC	4 cut(s)	366, 379, 1268, 3111
BspTNI	GGTCTC	1 cut(s)	20
BsrDI	GCAATG	1 cut(s)	592
BsrGI	TGTACA	2 cut(s)	677, 3094
BssMI	GATC	8 cut(s)	217, 371, 775, 1126, 1273, 1825, 2557, 3103
BssSI	CACGAG	1 cut(s)	3073
BssT1I	CCWWGG	3 cut(s)	95, 2683, 3040
Bst2BI	CACGAG	1 cut(s)	3073
Bst2UI	CCWGG	7 cut(s)	65, 744, 1265, 1871, 1982, 2060, 2076
Bst4CI	ACNGT	4 cut(s)	588, 634, 2141, 3136
Bst6I	CTCTTC	6 cut(s)	261, 397, 549, 888, 1539, 1849
BstAUI	TGTACA	2 cut(s)	677, 3094
BstBAI	YACGTR	1 cut(s)	1460
BstC8I	GCNNGC	6 cut(s)	1257, 1948, 1978, 2047, 2316, 2829
BstDEI	CTNAG	7 cut(s)	390, 687, 1093, 1206, 1875, 2018, 2028
BstDSI	CCRYGG	4 cut(s)	252, 855, 2683, 3040
BstF5I	GGATG	9 cut(s)	310, 565, 731, 1277, 2339, 2452, 2746, 2881, 2971
BstFNI	CGCG	1 cut(s)	3216
BstHHI	GCGC	2 cut(s)	2314, 3218
BstKTI	GATC	8 cut(s)	220, 374, 778, 1129, 1276, 1828, 2560, 3106
BstMAI	GTCTC	9 cut(s)	20, 471, 1136, 1562, 1618, 2208, 2288, 2368, 3142
BstMBI	GATC	8 cut(s)	217, 371, 775, 1126, 1273, 1825, 2557, 3103
BstNI	CCWGG	7 cut(s)	65, 744, 1265, 1871, 1982, 2060, 2076
BstNSI	RCATGY	2 cut(s)	2484, 2831
BstPAI	GACNNNNGTC	1 cut(s)	3186
BstSCI	CCNGG	9 cut(s)	63, 703, 742, 1263, 1869, 1980, 2058, 2074, 2086
BstSFI	CTRYAG	3 cut(s)	1941, 2208, 2799
BstSLI	GKGCMC	1 cut(s)	874
BstUI	CGCG	1 cut(s)	3216
BstV1I	GCAGC	6 cut(s)	192, 295, 1288, 1862, 3205, 3216
BstV2I	GAAGAC	5 cut(s)	11, 16, 1872, 2685, 2952
BstX2I	RGATCY	2 cut(s)	371, 1273
BstXI	CCANNNNNNTGG	1 cut(s)	2408
BstYI	RGATCY	2 cut(s)	371, 1273
BsuRI	GGCC	2 cut(s)	1656, 1980
BtgI	CCRYGG	4 cut(s)	252, 855, 2683, 3040
BtsCI	GGATG	9 cut(s)	310, 565, 731, 1277, 2339, 2452, 2746, 2881, 2971
BtsIMutI	CAGTG	7 cut(s)	727, 803, 888, 1288, 1387, 1864, 2331
BveI	ACCTGC	1 cut(s)	1611
Cac8I	GCNNGC	6 cut(s)	1257, 1948, 1978, 2047, 2316, 2829
CaiI	CAGNNNCTG	1 cut(s)	1100
CciI	TCATGA	3 cut(s)	334, 822, 2899
CfoI	GCGC	2 cut(s)	2314, 3218
Cfr13I	GGNCC	3 cut(s)	1934, 2992, 3170
CseI	GACGC	1 cut(s)	2534
Csp6I	GTAC	7 cut(s)	124, 576, 678, 879, 1466, 1788, 3095
CspCI	CAANNNNNGTGG	2 cut(s)	703, 738
CviQI	GTAC	7 cut(s)	124, 576, 678, 879, 1466, 1788, 3095
DdeI	CTNAG	7 cut(s)	390, 687, 1093, 1206, 1875, 2018, 2028
DpnI	GATC	8 cut(s)	219, 373, 777, 1128, 1275, 1827, 2559, 3105
DpnII	GATC	8 cut(s)	217, 371, 775, 1126, 1273, 1825, 2557, 3103
DriI	GACNNNNNGTC	1 cut(s)	12
Eam1104I	CTCTTC	6 cut(s)	261, 397, 549, 888, 1539, 1849
Eam1105I	GACNNNNNGTC	1 cut(s)	12
EarI	CTCTTC	6 cut(s)	261, 397, 549, 888, 1539, 1849
Ecl136II	GAGCTC	1 cut(s)	1681
Eco130I	CCWWGG	3 cut(s)	95, 2683, 3040
Eco24I	GRGCYC	1 cut(s)	1683
Eco31I	GGTCTC	1 cut(s)	20
Eco47I	GGWCC	3 cut(s)	1934, 2992, 3170
Eco53kI	GAGCTC	1 cut(s)	1681
Eco57I	CTGAAG	7 cut(s)	16, 912, 1208, 1596, 1830, 2697, 2964
Eco88I	CYCGRG	2 cut(s)	517, 3197
EcoICRI	GAGCTC	1 cut(s)	1681
EcoRII	CCWGG	7 cut(s)	63, 742, 1263, 1869, 1980, 2058, 2074
EcoT14I	CCWWGG	3 cut(s)	95, 2683, 3040
EcoT38I	GRGCYC	1 cut(s)	1683
ErhI	CCWWGG	3 cut(s)	95, 2683, 3040
FalI	AAGNNNNNCTT	4 cut(s)	258, 290, 526, 558
FaqI	GGGAC	2 cut(s)	1103, 1851
FauNDI	CATATG	1 cut(s)	1406
FblI	GTMKAC	1 cut(s)	3123
Fnu4HI	GCNGC	8 cut(s)	181, 309, 1302, 1851, 3060, 3219, 3230, 3233
FokI	GGATG	9 cut(s)	317, 572, 718, 1264, 2326, 2459, 2753, 2888, 2978
FriOI	GRGCYC	1 cut(s)	1683
Fsp4HI	GCNGC	8 cut(s)	181, 309, 1302, 1851, 3060, 3219, 3230, 3233
FspBI	CTAG	3 cut(s)	9, 96, 3206
GlaI	GCGC	2 cut(s)	2313, 3217
GluI	GCNGC	8 cut(s)	181, 309, 1302, 1851, 3060, 3219, 3230, 3233
GsuI	CTGGAG	6 cut(s)	47, 119, 1411, 2042, 2205, 3228
HaeIII	GGCC	2 cut(s)	1656, 1980
HapII	CCGG	2 cut(s)	705, 2088
HgaI	GACGC	1 cut(s)	2534
HhaI	GCGC	2 cut(s)	2314, 3218
Hin6I	GCGC	2 cut(s)	2312, 3216
HinP1I	GCGC	2 cut(s)	2312, 3216
HincII	GTYRAC	3 cut(s)	211, 693, 2836
HindII	GTYRAC	3 cut(s)	211, 693, 2836
HpaI	GTTAAC	1 cut(s)	693
HpaII	CCGG	2 cut(s)	705, 2088
HphI	GGTGA	4 cut(s)	614, 785, 1430, 2151
Hpy166II	GTNNAC	6 cut(s)	211, 693, 872, 2452, 2836, 3124
Hpy8I	GTNNAC	6 cut(s)	211, 693, 872, 2452, 2836, 3124
HpyAV	CCTTC	3 cut(s)	2408, 2540, 3245
HpyCH4III	ACNGT	4 cut(s)	588, 634, 2141, 3136
HpyCH4IV	ACGT	3 cut(s)	1459, 2475, 2838
HpyF3I	CTNAG	7 cut(s)	390, 687, 1093, 1206, 1875, 2018, 2028
HpySE526I	ACGT	3 cut(s)	1459, 2475, 2838
HspAI	GCGC	2 cut(s)	2312, 3216
KspAI	GTTAAC	1 cut(s)	693
Kzo9I	GATC	8 cut(s)	217, 371, 775, 1126, 1273, 1825, 2557, 3103
Lsp1109I	GCAGC	6 cut(s)	192, 295, 1288, 1862, 3205, 3216
MaeI	CTAG	3 cut(s)	9, 96, 3206
MaeII	ACGT	3 cut(s)	1459, 2475, 2838
MaeIII	GTNAC	6 cut(s)	444, 521, 1889, 2258, 2476, 3265
MalI	GATC	8 cut(s)	219, 373, 777, 1128, 1275, 1827, 2559, 3105
MboI	GATC	8 cut(s)	217, 371, 775, 1126, 1273, 1825, 2557, 3103
MfeI	CAATTG	2 cut(s)	261, 1023
MflI	RGATCY	2 cut(s)	371, 1273
MhlI	GDGCHC	2 cut(s)	874, 1683
MlyI	GAGTC	8 cut(s)	340, 518, 1085, 1833, 2210, 2437, 3104, 3209
MmeI	TCCRAC	1 cut(s)	1761
MroXI	GAANNNNTTC	1 cut(s)	406
MseI	TTAA	9 cut(s)	86, 692, 1152, 1746, 1805, 2037, 2145, 2304, 2486
MslI	CAYNNNNRTG	7 cut(s)	225, 430, 998, 1416, 1437, 2565, 2904
MspI	CCGG	2 cut(s)	705, 2088
MspR9I	CCNGG	9 cut(s)	65, 705, 744, 1265, 1871, 1982, 2060, 2076, 2088
MunI	CAATTG	2 cut(s)	261, 1023
Mva1269I	GAATGC	2 cut(s)	856, 964
MvaI	CCWGG	7 cut(s)	65, 744, 1265, 1871, 1982, 2060, 2076
MvnI	CGCG	1 cut(s)	3216
NciI	CCSGG	2 cut(s)	705, 2088
NcoI	CCATGG	2 cut(s)	2683, 3040
NdeI	CATATG	1 cut(s)	1406
NdeII	GATC	8 cut(s)	217, 371, 775, 1126, 1273, 1825, 2557, 3103
NlaIV	GGNNCC	8 cut(s)	154, 373, 748, 1223, 1394, 2080, 2716, 3063
NmuCI	GTSAC	4 cut(s)	521, 2258, 2476, 3265
NspI	RCATGY	2 cut(s)	2484, 2831
PaeI	GCATGC	1 cut(s)	2831
PaeR7I	CTCGAG	1 cut(s)	517
PagI	TCATGA	3 cut(s)	334, 822, 2899
PciI	ACATGT	1 cut(s)	2480
PcsI	WCGNNNNNNNCGW	2 cut(s)	24, 33
PctI	GAATGC	2 cut(s)	856, 964
PdmI	GAANNNNTTC	1 cut(s)	406
PflMI	CCANNNNNTGG	2 cut(s)	1675, 3046
PfoI	TCCNGGA	1 cut(s)	2058
PkrI	GCNGC	8 cut(s)	182, 310, 1303, 1852, 3061, 3220, 3231, 3234
PleI	GAGTC	8 cut(s)	339, 518, 1085, 1833, 2210, 2436, 3104, 3208
PpsI	GAGTC	8 cut(s)	339, 518, 1085, 1833, 2210, 2436, 3104, 3208
Ppu21I	YACGTR	1 cut(s)	1460
PscI	ACATGT	1 cut(s)	2480
PshAI	GACNNNNGTC	1 cut(s)	3186
PshBI	ATTAAT	1 cut(s)	1152
PsiI	TTATAA	1 cut(s)	2643
Psp124BI	GAGCTC	1 cut(s)	1683
Psp6I	CCWGG	7 cut(s)	63, 742, 1263, 1869, 1980, 2058, 2074
PspGI	CCWGG	7 cut(s)	63, 742, 1263, 1869, 1980, 2058, 2074
PspN4I	GGNNCC	8 cut(s)	154, 373, 748, 1223, 1394, 2080, 2716, 3063
PspPI	GGNCC	3 cut(s)	1934, 2992, 3170
PspXI	VCTCGAGB	1 cut(s)	517
PstNI	CAGNNNCTG	1 cut(s)	1100
PsuI	RGATCY	2 cut(s)	371, 1273
RsaI	GTAC	7 cut(s)	125, 577, 679, 880, 1467, 1789, 3096
RsaNI	GTAC	7 cut(s)	124, 576, 678, 879, 1466, 1788, 3095
RseI	CAYNNNNRTG	7 cut(s)	225, 430, 998, 1416, 1437, 2565, 2904
SacI	GAGCTC	1 cut(s)	1683
SaqAI	TTAA	9 cut(s)	86, 692, 1152, 1746, 1805, 2037, 2145, 2304, 2486
SatI	GCNGC	8 cut(s)	181, 309, 1302, 1851, 3060, 3219, 3230, 3233
Sau3AI	GATC	8 cut(s)	217, 371, 775, 1126, 1273, 1825, 2557, 3103
Sau96I	GGNCC	3 cut(s)	1934, 2992, 3170
ScaI	AGTACT	1 cut(s)	880
SchI	GAGTC	8 cut(s)	340, 518, 1085, 1833, 2210, 2437, 3104, 3209
ScrFI	CCNGG	9 cut(s)	65, 705, 744, 1265, 1871, 1982, 2060, 2076, 2088
SduI	GDGCHC	2 cut(s)	874, 1683
SfcI	CTRYAG	3 cut(s)	1941, 2208, 2799
Sfr274I	CTCGAG	1 cut(s)	517
SinI	GGWCC	3 cut(s)	1934, 2992, 3170
SlaI	CTCGAG	1 cut(s)	517
SmiMI	CAYNNNNRTG	7 cut(s)	225, 430, 998, 1416, 1437, 2565, 2904
SmlI	CTYRAG	9 cut(s)	458, 517, 1619, 1781, 1971, 2553, 2592, 2763, 2853
SmoI	CTYRAG	9 cut(s)	458, 517, 1619, 1781, 1971, 2553, 2592, 2763, 2853
SphI	GCATGC	1 cut(s)	2831
SsiI	CCGC	6 cut(s)	12, 1628, 2318, 2387, 3059, 3232
SspI	AATATT	1 cut(s)	74
SspMI	CTAG	3 cut(s)	9, 96, 3206
SstI	GAGCTC	1 cut(s)	1683
StyD4I	CCNGG	9 cut(s)	63, 703, 742, 1263, 1869, 1980, 2058, 2074, 2086
StyI	CCWWGG	3 cut(s)	95, 2683, 3040
TaaI	ACNGT	4 cut(s)	588, 634, 2141, 3136
TaiI	ACGT	3 cut(s)	1462, 2478, 2841
TaqI	TCGA	7 cut(s)	18, 27, 102, 518, 930, 1647, 2426
TatI	WGTACW	3 cut(s)	677, 878, 3094
TauI	GCSGC	2 cut(s)	3062, 3235
Tru1I	TTAA	9 cut(s)	86, 692, 1152, 1746, 1805, 2037, 2145, 2304, 2486
Tru9I	TTAA	9 cut(s)	86, 692, 1152, 1746, 1805, 2037, 2145, 2304, 2486
TscAI	CASTG	7 cut(s)	727, 803, 895, 1288, 1394, 1864, 2338
TseFI	GTSAC	4 cut(s)	521, 2258, 2476, 3265
TseI	GCWGC	6 cut(s)	180, 308, 1301, 1850, 3218, 3229
Tsp45I	GTSAC	4 cut(s)	521, 2258, 2476, 3265
TspGWI	ACGGA	4 cut(s)	459, 872, 1755, 2637
TspRI	CASTG	7 cut(s)	727, 803, 895, 1288, 1394, 1864, 2338
Van91I	CCANNNNNTGG	2 cut(s)	1675, 3046
VneI	GTGCAC	1 cut(s)	870
VpaK11BI	GGWCC	3 cut(s)	1934, 2992, 3170
VspI	ATTAAT	1 cut(s)	1152
XapI	RAATTY	6 cut(s)	115, 815, 1048, 1453, 1639, 3159
XceI	RCATGY	2 cut(s)	2484, 2831
XhoI	CTCGAG	1 cut(s)	517
XmaJI	CCTAGG	1 cut(s)	95
XmiI	GTMKAC	1 cut(s)	3123
XmnI	GAANNNNTTC	1 cut(s)	406
XspI	CTAG	3 cut(s)	9, 96, 3206
ZrmI	AGTACT	1 cut(s)	880

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.