Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0002627

Physical Location & Seq

Genomic Coordinates Reverse (-)

61454869 .. 61468737

Gene Structure 13869 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ21219

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3732 bp

ATGAATAGCGCACCGCCGCGGAGAGGCCTCGAAGTCTACGATTTCGTCGACGAGGACGAGTTCCCGGACCCGGTCGTCAAATTTTCCGGCAAACTCAAAAACCCTAGCCGCGAGGACCATCGGAATTCGAAGGACGAGTTTCTCCAACCTGGTTCGAAAGAAGCCAACGTTCAAGCCAAAGAAATCAGTAGCATACCTTGTGTAGAAGTTGATGAAGTTGACACTGACCACTGTTGTAAAAATGCTATTTCTCATTCTCCTCGGTATACAATTGAAGAGAGGCTTGCCATCGTAAATGATTTGAAACTCAGTGCTGCCACAGACTCCAGTCATGAACAATCTGATTTATGTTCGAAAGAAGCCAACGTTCAAGCCAAAGAAATCAGTAGCATACCTTGTGTAGGTGTTGATGAAGTTGACACTGACCACTGTTGTAAAAATGCTATTTCTCATTCTCCTCTGTATACAATTGAAGAGAGGCGTGCTATCATAAACGATTTGAAATTGGGTGCTGCCACAGACTCCAGTCATGAACAACAATCTGATTTATGTTCGAAAGAAGCCAACGTTCAAGCCAAAGAAATCAGTAGCATACCTTGTGTAGGTGTTGATGAAGTTGACACTGACCACTGTTGTAAAAATGCTATTTCTCATTCTCCTCTGTATACAATTGAAGAGAGGCGTGCTATCATAAACGATTTGAAATTGGGTGCTGCCACAGACTCCAGTCATGAACAACAATCTGATTTATGTTCGAAAGAAGCCAACGTTCAAGCCAAAGAAATCAGTAGCATACCCTGTGTAGATGTTGATGAAGTTGACACTGACCACTGTTGTAAAAATGCTATTTCTCATTCTACTCGGTATACAATTGAAGAGAGGCTTGCTATCATAAATGATTTGAAATTGAGTGCTGCCACAGACTCCAGTCATGAACAACAATCTGATTTGGACAAAGTCAGTAACATACTTTATGCAGGGGTTGATGCAATGGACCATGATCATTGCAGTGATAGTGCTAGTTTAGATTCCCGCCCGGGCACAATGGAAGAGGATCCGGCTACAAAAAAGGATTATTTGGAATTGGATGCTGCTACACAATCCAAATTTTTGAGTCATGAACAACAGTCTGAATTAGATGACAATGGACGCAGAAAGTTTCTTTCTGAAACAGAGACTGGCGGTTCAGATGTTCCCTCACCTTCCCCCCAGACAAGCCAGTTAGACTGTGACCTTACAGTGTCCCCTTCCAGTAACGACCCAGTTGATGTTATTTCAGATGCTGATGAAAGCACAAGTGAGTCTCCATCAAGTCCCCTATCCCCAATTGAAGAGGATGACGGTTCTTTGGGTACTTATGAATTGGGTCATTGCTCAGGCAATTTTGAAATGGATAACATAAATATGACGGTTGTTCTTTATCCGGATTATGTTGTATATGGGGATAGTTATTGTACAGGAGCCCAGCAGTTAACCTTCTCTCACAGTTGCATCAAAATCAGTGGTTTAGCACTGTCTGAAAACGACAAAACTTTGAATTTTGAATGGGGAGTTGATGATCTTGTCAATGTTGAGTGTCAGTGGGTTCAAAATGCTGAGTTTGTCATGATAAAACTCCATGTACTATCAAAGGATGCAGATGGAGATGATGGTGCACTTGGTATTTCAGGCATTGAGGAGTTGAAAATTGGAGTTGTTGAACCCAACTGGTCACAGCAACAAGAAAGAATAGCATGTTTGAATGATAAATATTTGGATATATTGGAACCGGTGCAAATCATGGAAGCGGACAATTCACTTGGCCAGAGGCATCATTTTCCAAATTTCGACGAGGATTTTGAAACTGTTGTATATCCGGAGGGGGATCCTGATGCCGTTTCCATCAGTAAGAGAGATGTTGATCTCTTACAGCCAGAGATATTTATTAACGATACATTGATCGACTTCTATATCAAGTATTTGGAAAATCGGATTCAACCCGAGGAAAAGCACAGGTTCTACTTTTTCAATAGTTTTTTCTTTCGAAAGCTGGCTGACCTGGACAAGGATCCATCCAGTGTTCCTGGTGGCAGGGCTGCTTTCCAACGTGTTCGGAAATGGACAAGGAAAGTGGATTTGTTTGAAAAGGATTACATCTTCATCCCTGTAAACTTCAATCTACACTGGACTCTAATTGTTATATGTCATCCTGGTGAAGTGCCTAGATATAAGGTTGGAGACTCAGGCAAGTCAGTTAAGGTACCTTGTATATTGCACTTGGATTCTCTCAAAGGAAGTCATACAGGTCTCAAAAATCATGTTCAAAGTTATTTGTGGGAAGAGTGGAAAGAAAAGAAAAAGGAGACATCGGAAGAAATCTCATCAAACTTTCACAACCTGCGGTTTCTCTCACTGGAGCTACCACAACAGGAAAATACATATGATTGTGGCCTGTTTTTGCTTCACTATCTAGAGCTGTTTTTGGAAGAAGCTCCTGCTATCTTCAATCCATTCGACATAAACAAGTTCTCCACCTTTCTTAATGCAAATTGGTTTCTTCCTTCCGAGGCTTCTCTCAAGCGCACTCTTATCCAGAGATTAATTTTTGATCTCATTGAAAATCGTTATCGGGAAGTCTGTTTGCCAGCTTGCAGTGATGGAGATCAGTCTAAATATCAGGGATGTAATCAGCTTGAAATAGGTGCAAAGTTTCTTTCTGGAAGATCCAGTCCTGCTATAGCTTGCCAAGAAAATATATCAACTTCTCAAGCAGACCACGGAATCGAAATGACTCTGTTACCCACATCTTCTCTTAGGAGTTCTGAGTGTGTTAGTGATGCAGGCCTGGTTCTCAGGGAATTTTTTCAGTCAGGAGAAACTGCAGGTTCACTATTTGGACAATATCAATCCTTCGAACAGAAATCGCCTTTTTACCATCCTAATGGAGCTACACCACCAGTAGAGGAAGAAACCCAAACTGGAGAGCAGTTTGCCTATTTGGCTACGGGAGACACTGGCTTTCAAGAGATGTCTACAATCACATCTCAAACTTGTGGTATTCAATGCTCTTCGAAATCTTATGGAGTTGAGACTTCGTATAACTTGGGGGTGTCTGAACAGGCACCAAATGGAAACATTGATGCATCCCCTGACCGATCAGTTTGTGCCTCTGATCTTTCTGAAGGCATAGAGATCATTGAAAATGGTTCAGTTAGAGAAGATTTAGGTCCAGGCCAGAAAGAAGAAATGGATGTACAAAATTATCCATCATTGGACAGTGTCACGTGTGTGGTAGATGGCCTTGTTTCTGGTTTGGGTGAGGTGCAGAGCGGATCCTTGATTGAAGAAGGTTCTCAGGATCATGAAGGGGTGCATGACGCACTTGAAAATGGAGGTCCAAGTCGGAAAGAAGAAAGGGATGTACAGACCCATTCATCAACAGAAGATGTCTTGTGTCTAGCAGATGGCCTTGTATCTGGTTCAGGCAATATGCAGAGCACTTCTGTTACTGAAGAAAGTTCTCTGGACCAAAAAAAGGTGCATGTTGGAAATGAAAATAGTGCTTCACTTCCCTTATCCCAAGAAATTTCTGATATACCCGTTCTTGAAGACGTTGACATGGTAGGCAATGGTACAATCTCATGTGATAATGGTTTGGTGGGCGAGTCACAGGAGCCAGAGTCTGAAGAGCAACGAGCTGCAAAAAGAATGAGGCTTACACCTCCAGTTGAAGGGGAAAAATGTCTCACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0006464 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009987 GO:0016787 GO:0016926 GO:0016929 GO:0018193 GO:0018205 GO:0019538 GO:0019783 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0070011 GO:0070137 GO:0070139 GO:0070646 GO:0070647 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_C48

Protein Analysis

1243

Amino Acids

137.38

Weight (kDa)

4.47

Isoelectric Point (pI)

50.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PH_ULP	PF25352	469 - 587	7.2e-44	Ubiquitin-like protease PH domain
Peptidase_C48	PF02902	642 - 840	1.4e-35	Ulp1 protease family, C-terminal catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0002627)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09730 AT1G09730
fragaria_vesca	FvH4_3g25421 FvH4_5g35390 FvH4_5g35390 FvH4_5g35390 FvH4_5g35390
malus_domestica	MD08G1197400.v1.1 MD15G1385000.v1.1
prunus_persica	Prupe.6G149300_v2.0.a1 Prupe.6G149300_v2.0.a1 Prupe.6G149300_v2.0.a1 Prupe.6G149300_v2.0.a1 Prupe.6G149300_v2.0.a1 Prupe.6G149300_v2.0.a1 Prupe.6G149300_v2.0.a1 Prupe.6G149300_v2.0.a1 Prupe.6G149300_v2.0.a1 Prupe.6G149300_v2.0.a1 Prupe.6G149300_v2.0.a1 Prupe.6G149300_v2.0.a1 Prupe.6G149300_v2.0.a1
pyrus_communis	pycom08g16950 pycom08g16970 pycom15g34500
rosa_chinensis	RchiOBHm_Chr7g0236781
rosa_laevigata	RLG00000001062
rosa_multiflora	Rmu_sc0003720.1_g000028
rosa_roxburghii	Rroxscaffold_3G00224860
rosa_rugosa	Rorug07G0298800 Rorug07G0298900 Rorug07G0299000.1 Rorug07G0299100 Rorug07G0299200
rosa_samantha	Rh7AG454400 Rh7BG425600 Rh7CG472900 Rh7DG442100
rosa_wichuraiana	Rw7G037660 Rw7G037720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	74
Acc36I	ACCTGC	2 cut(s)	2385, 2852
Acc65I	GGTACC	1 cut(s)	2239
AccB1I	GGYRCC	2 cut(s)	2239, 3100
AccBSI	CCGCTC	1 cut(s)	3309
AccI	GTMKAC	7 cut(s)	36, 48, 266, 464, 665, 866, 3011
AccII	CGCG	2 cut(s)	19, 111
AccIII	TCCGGA	2 cut(s)	1423, 1855
AciI	CCGC	9 cut(s)	14, 17, 19, 109, 1033, 1182, 1787, 2380, 3309
AclI	AACGTT	4 cut(s)	168, 366, 567, 768
AcoI	YGGCCR	1 cut(s)	1801
AcsI	RAATTY	7 cut(s)	80, 124, 1106, 1537, 1822, 2837, 3564
AcuI	CTGAAG	3 cut(s)	3180, 3510, 3684
AcvI	CACGTG	1 cut(s)	3264
AfaI	GTAC	7 cut(s)	1354, 1456, 1623, 2241, 3234, 3402, 3613
AfiI	CCNNNNNNNGG	7 cut(s)	23, 70, 401, 602, 2044, 3514, 3710
AflIII	ACRYGT	2 cut(s)	2086, 3263
AgeI	ACCGGT	1 cut(s)	1768
AjnI	CCWGG	6 cut(s)	148, 2037, 2062, 2188, 2823, 3208
AleI	CACNNNNGTG	1 cut(s)	3266
AluBI	AGCT	9 cut(s)	2029, 2398, 2455, 2471, 2627, 2671, 2720, 2927, 3677
AluI	AGCT	9 cut(s)	2029, 2398, 2455, 2471, 2627, 2671, 2720, 2927, 3677
Alw21I	GWGCWC	2 cut(s)	1657, 3479
Alw26I	GTCTC	8 cut(s)	1169, 1308, 2211, 2291, 2336, 2982, 3062, 3728
Alw44I	GTGCAC	1 cut(s)	1653
AlwNI	CAGNNNCTG	3 cut(s)	1178, 1283, 3662
Ama87I	CYCGRG	2 cut(s)	1036, 1979
Aor13HI	TCCGGA	2 cut(s)	1423, 1855
AoxI	GGCC	7 cut(s)	25, 1801, 2428, 2821, 3211, 3277, 3445
ApaLI	GTGCAC	1 cut(s)	1653
ApeKI	GCWGC	7 cut(s)	314, 512, 713, 914, 1091, 2075, 3677
ApoI	RAATTY	7 cut(s)	80, 124, 1106, 1537, 1822, 2837, 3564
ArsI	GACNNNNNNTTYG	2 cut(s)	1820, 1852
AseI	ATTAAT	1 cut(s)	2579
AsiGI	ACCGGT	1 cut(s)	1768
Asp700I	GAANNNNTTC	1 cut(s)	2841
Asp718I	GGTACC	1 cut(s)	2239
AspLEI	GCGC	2 cut(s)	11, 2562
AspS9I	GGNCC	6 cut(s)	67, 115, 994, 3206, 3374, 3505
AsuC2I	CCSGG	4 cut(s)	65, 71, 1037, 1038
AsuHPI	GGTGA	3 cut(s)	1191, 2204, 3308
AsuII	TTCGAA	8 cut(s)	128, 155, 353, 554, 755, 2023, 2892, 3050
AvaI	CYCGRG	2 cut(s)	1036, 1979
AvaII	GGWCC	6 cut(s)	67, 115, 994, 3206, 3374, 3505
BaeGI	GKGCMC	2 cut(s)	1043, 1657
BalI	TGGCCA	1 cut(s)	1803
BamHI	GGATCC	4 cut(s)	1054, 1864, 2047, 3311
BanI	GGYRCC	2 cut(s)	2239, 3100
BanII	GRGCYC	1 cut(s)	1465
BbrPI	CACGTG	1 cut(s)	3264
BbsI	GAAGAC	1 cut(s)	3594
Bbv12I	GWGCWC	2 cut(s)	1657, 3479
BbvI	GCAGC	7 cut(s)	301, 499, 700, 901, 1078, 2062, 3664
BceAI	ACGGC	1 cut(s)	1859
BciT130I	CCWGG	6 cut(s)	150, 2039, 2064, 2190, 2825, 3210
BclI	TGATCA	1 cut(s)	1000
BcnI	CCSGG	4 cut(s)	65, 71, 1037, 1038
BcoDI	GTCTC	8 cut(s)	1169, 1308, 2211, 2291, 2336, 2982, 3062, 3728
BfaI	CTAG	5 cut(s)	105, 1020, 2202, 2450, 3437
BfmI	CTRYAG	2 cut(s)	2715, 2859
BfuAI	ACCTGC	2 cut(s)	2385, 2852
BglI	GCCNNNNNGGC	1 cut(s)	2978
BisI	GCNGC	9 cut(s)	17, 109, 315, 513, 714, 915, 1092, 2076, 3678
BlsI	GCNGC	9 cut(s)	18, 110, 316, 514, 715, 916, 1093, 2077, 3679
Bme18I	GGWCC	6 cut(s)	67, 115, 994, 3206, 3374, 3505
BmeT110I	CYCGRG	2 cut(s)	1036, 1979
BmgT120I	GGNCC	6 cut(s)	67, 115, 994, 3206, 3374, 3505
BmrI	ACTGGG	1 cut(s)	1256
BmuI	ACTGGG	1 cut(s)	1256
BoxI	GACNNNNGTC	4 cut(s)	327, 525, 726, 927
BpiI	GAAGAC	1 cut(s)	3594
BpmI	CTGGAG	7 cut(s)	310, 508, 709, 910, 2414, 2979, 3687
Bpu10I	CCTNAGC	1 cut(s)	1375
Bpu14I	TTCGAA	8 cut(s)	128, 155, 353, 554, 755, 2023, 2892, 3050
BpuEI	CTTGAG	2 cut(s)	2540, 2730
BpuMI	CCSGG	4 cut(s)	65, 71, 1037, 1038
BsaAI	YACGTR	1 cut(s)	3264
BsaBI	GATNNNNATC	2 cut(s)	1899, 2640
BsaI	GGTCTC	1 cut(s)	2291
BsaJI	CCNNGG	6 cut(s)	17, 260, 1036, 1980, 2544, 2755
BsaWI	WCCGGW	3 cut(s)	1423, 1768, 1855
BsaXI	ACNNNNNCTCC	4 cut(s)	2492, 2522, 3644, 3674
Bsc4I	CCNNNNNNNGG	7 cut(s)	23, 70, 401, 602, 2044, 3514, 3710
Bse118I	RCCGGY	1 cut(s)	1768
Bse3DI	GCAATG	4 cut(s)	996, 1003, 1369, 3613
Bse8I	GATNNNNATC	2 cut(s)	1899, 2640
BseAI	TCCGGA	2 cut(s)	1423, 1855
BseBI	CCWGG	6 cut(s)	150, 2039, 2064, 2190, 2825, 3210
BseDI	CCNNGG	6 cut(s)	17, 260, 1036, 1980, 2544, 2755
BseJI	GATNNNNATC	2 cut(s)	1899, 2640
BseLI	CCNNNNNNNGG	7 cut(s)	23, 70, 401, 602, 2044, 3514, 3710
BseMI	GCAATG	4 cut(s)	996, 1003, 1369, 3613
BseMII	CTCAG	7 cut(s)	322, 1389, 1587, 2235, 2793, 2845, 3347
BseRI	GAGGAG	4 cut(s)	249, 447, 648, 1691
BseSI	GKGCMC	2 cut(s)	1043, 1657
BseXI	GCAGC	7 cut(s)	301, 499, 700, 901, 1078, 2062, 3664
BseYI	CCCAGC	1 cut(s)	1464
BsgI	GTGCAG	1 cut(s)	3323
Bsh1236I	CGCG	2 cut(s)	19, 111
Bsh1285I	CGRYCG	1 cut(s)	75
BshFI	GGCC	7 cut(s)	27, 1803, 2430, 2823, 3213, 3279, 3447
BshNI	GGYRCC	2 cut(s)	2239, 3100
BshTI	ACCGGT	1 cut(s)	1768
BsiEI	CGRYCG	1 cut(s)	75
BsiHKAI	GWGCWC	2 cut(s)	1657, 3479
BsiHKCI	CYCGRG	2 cut(s)	1036, 1979
BsiSI	CCGG	8 cut(s)	65, 71, 87, 1037, 1058, 1424, 1769, 1856
BslFI	GGGAC	2 cut(s)	1228, 1299
BslI	CCNNNNNNNGG	7 cut(s)	23, 70, 401, 602, 2044, 3514, 3710
BsmAI	GTCTC	8 cut(s)	1169, 1308, 2211, 2291, 2336, 2982, 3062, 3728
BsmFI	GGGAC	2 cut(s)	1228, 1299
BsnI	GGCC	7 cut(s)	27, 1803, 2430, 2823, 3213, 3279, 3447
Bso31I	GGTCTC	1 cut(s)	2291
BsoBI	CYCGRG	2 cut(s)	1036, 1979
Bsp119I	TTCGAA	8 cut(s)	128, 155, 353, 554, 755, 2023, 2892, 3050
Bsp1286I	GDGCHC	4 cut(s)	1043, 1465, 1657, 3479
Bsp13I	TCCGGA	2 cut(s)	1423, 1855
Bsp1407I	TGTACA	3 cut(s)	1454, 3232, 3400
BspACI	CCGC	9 cut(s)	14, 17, 19, 109, 1033, 1182, 1787, 2380, 3309
BspANI	GGCC	7 cut(s)	27, 1803, 2430, 2823, 3213, 3279, 3447
BspCNI	CTCAG	7 cut(s)	321, 1388, 1588, 2234, 2794, 2844, 3346
BspEI	TCCGGA	2 cut(s)	1423, 1855
BspFNI	CGCG	2 cut(s)	19, 111
BspHI	TCATGA	7 cut(s)	331, 529, 730, 931, 1117, 1605, 3340
BspMAI	CTGCAG	1 cut(s)	2863
BspMI	ACCTGC	2 cut(s)	2385, 2852
BspQI	GCTCTTC	2 cut(s)	3052, 3660
BspT104I	TTCGAA	8 cut(s)	128, 155, 353, 554, 755, 2023, 2892, 3050
BspT107I	GGYRCC	2 cut(s)	2239, 3100
BspTNI	GGTCTC	1 cut(s)	2291
BsrBI	CCGCTC	1 cut(s)	3309
BsrDI	GCAATG	4 cut(s)	996, 1003, 1369, 3613
BsrFI	RCCGGY	1 cut(s)	1768
BsrGI	TGTACA	3 cut(s)	1454, 3232, 3400
BssAI	RCCGGY	1 cut(s)	1768
BssECI	CCNNGG	6 cut(s)	17, 260, 1036, 1980, 2544, 2755
BssNAI	GTATAC	4 cut(s)	267, 465, 666, 867
Bst1107I	GTATAC	4 cut(s)	267, 465, 666, 867
Bst2UI	CCWGG	6 cut(s)	150, 2039, 2064, 2190, 2825, 3210
Bst6I	CTCTTC	9 cut(s)	270, 468, 669, 870, 1044, 1326, 2313, 3052, 3660
BstAUI	TGTACA	3 cut(s)	1454, 3232, 3400
BstBAI	YACGTR	1 cut(s)	3264
BstBI	TTCGAA	8 cut(s)	128, 155, 353, 554, 755, 2023, 2892, 3050
BstC8I	GCNNGC	9 cut(s)	285, 483, 684, 885, 2031, 2625, 2629, 2722, 2821
BstDEI	CTNAG	8 cut(s)	308, 1375, 1596, 2221, 2792, 2802, 2831, 3333
BstDSI	CCRYGG	2 cut(s)	17, 2755
BstENI	CCTNNNNNAGG	3 cut(s)	399, 600, 2042
BstFNI	CGCG	2 cut(s)	19, 111
BstHHI	GCGC	2 cut(s)	11, 2562
BstMAI	GTCTC	8 cut(s)	1169, 1308, 2211, 2291, 2336, 2982, 3062, 3728
BstMCI	CGRYCG	1 cut(s)	75
BstMWI	GCNNNNNNNGC	1 cut(s)	2978
BstNI	CCWGG	6 cut(s)	150, 2039, 2064, 2190, 2825, 3210
BstNSI	RCATGY	2 cut(s)	1737, 3524
BstPAI	GACNNNNGTC	4 cut(s)	327, 525, 726, 927
BstSFI	CTRYAG	2 cut(s)	2715, 2859
BstSLI	GKGCMC	2 cut(s)	1043, 1657
BstUI	CGCG	2 cut(s)	19, 111
BstV1I	GCAGC	7 cut(s)	301, 499, 700, 901, 1078, 2062, 3664
BstV2I	GAAGAC	1 cut(s)	3594
BstX2I	RGATCY	5 cut(s)	1054, 1864, 2047, 2702, 3311
BstXI	CCANNNNNNTGG	1 cut(s)	2921
BstYI	RGATCY	5 cut(s)	1054, 1864, 2047, 2702, 3311
BstZ17I	GTATAC	4 cut(s)	267, 465, 666, 867
BsuRI	GGCC	7 cut(s)	27, 1803, 2430, 2823, 3213, 3279, 3447
BtgI	CCRYGG	2 cut(s)	17, 2755
BtsI	GCAGTG	2 cut(s)	1015, 2638
BveI	ACCTGC	2 cut(s)	2385, 2852
Cac8I	GCNNGC	9 cut(s)	285, 483, 684, 885, 2031, 2625, 2629, 2722, 2821
CaiI	CAGNNNCTG	3 cut(s)	1178, 1283, 3662
CciI	TCATGA	7 cut(s)	331, 529, 730, 931, 1117, 1605, 3340
CfoI	GCGC	2 cut(s)	11, 2562
Cfr10I	RCCGGY	1 cut(s)	1768
Cfr13I	GGNCC	6 cut(s)	67, 115, 994, 3206, 3374, 3505
Cfr42I	CCGCGG	1 cut(s)	20
Cfr9I	CCCGGG	1 cut(s)	1036
CseI	GACGC	2 cut(s)	1158, 3365
CsiI	ACCWGGT	1 cut(s)	148
Csp6I	GTAC	7 cut(s)	1353, 1455, 1622, 2240, 3233, 3401, 3612
CspAI	ACCGGT	1 cut(s)	1768
CspCI	CAANNNNNGTGG	4 cut(s)	1483, 1518, 2091, 2126
CviQI	GTAC	7 cut(s)	1353, 1455, 1622, 2240, 3233, 3401, 3612
DdeI	CTNAG	8 cut(s)	308, 1375, 1596, 2221, 2792, 2802, 2831, 3333
DrdI	GACNNNNNNGTC	1 cut(s)	74
DseDI	GACNNNNNNGTC	1 cut(s)	74
EaeI	YGGCCR	1 cut(s)	1801
Eam1104I	CTCTTC	9 cut(s)	270, 468, 669, 870, 1044, 1326, 2313, 3052, 3660
EarI	CTCTTC	9 cut(s)	270, 468, 669, 870, 1044, 1326, 2313, 3052, 3660
Eco147I	AGGCCT	2 cut(s)	27, 2823
Eco24I	GRGCYC	1 cut(s)	1465
Eco31I	GGTCTC	1 cut(s)	2291
Eco47I	GGWCC	6 cut(s)	67, 115, 994, 3206, 3374, 3505
Eco57I	CTGAAG	3 cut(s)	3180, 3510, 3684
Eco72I	CACGTG	1 cut(s)	3264
Eco88I	CYCGRG	2 cut(s)	1036, 1979
EcoNI	CCTNNNNNAGG	3 cut(s)	399, 600, 2042
EcoRI	GAATTC	1 cut(s)	124
EcoRII	CCWGG	6 cut(s)	148, 2037, 2062, 2188, 2823, 3208
EcoT22I	ATGCAT	1 cut(s)	3124
EcoT38I	GRGCYC	1 cut(s)	1465
FalI	AAGNNNNNCTT	4 cut(s)	267, 299, 867, 899
FaqI	GGGAC	2 cut(s)	1228, 1299
FauI	CCCGC	1 cut(s)	1040
FauNDI	CATATG	1 cut(s)	2419
FbaI	TGATCA	1 cut(s)	1000
FblI	GTMKAC	7 cut(s)	36, 48, 266, 464, 665, 866, 3011
Fnu4HI	GCNGC	9 cut(s)	17, 109, 315, 513, 714, 915, 1092, 2076, 3678
FriOI	GRGCYC	1 cut(s)	1465
Fsp4HI	GCNGC	9 cut(s)	17, 109, 315, 513, 714, 915, 1092, 2076, 3678
FspBI	CTAG	5 cut(s)	105, 1020, 2202, 2450, 3437
GlaI	GCGC	2 cut(s)	10, 2561
GluI	GCNGC	9 cut(s)	17, 109, 315, 513, 714, 915, 1092, 2076, 3678
GsaI	CCCAGC	1 cut(s)	1468
GsuI	CTGGAG	7 cut(s)	310, 508, 709, 910, 2414, 2979, 3687
HaeIII	GGCC	7 cut(s)	27, 1803, 2430, 2823, 3213, 3279, 3447
HapII	CCGG	8 cut(s)	65, 71, 87, 1037, 1058, 1424, 1769, 1856
HgaI	GACGC	2 cut(s)	1158, 3365
HhaI	GCGC	2 cut(s)	11, 2562
Hin6I	GCGC	2 cut(s)	9, 2560
HinP1I	GCGC	2 cut(s)	9, 2560
HincII	GTYRAC	7 cut(s)	49, 220, 418, 619, 820, 1473, 3595
HindII	GTYRAC	7 cut(s)	49, 220, 418, 619, 820, 1473, 3595
HpaI	GTTAAC	1 cut(s)	1473
HpaII	CCGG	8 cut(s)	65, 71, 87, 1037, 1058, 1424, 1769, 1856
HphI	GGTGA	3 cut(s)	1191, 2204, 3308
Hpy99I	CGWCG	3 cut(s)	50, 53, 1832
HpyCH4IV	ACGT	7 cut(s)	168, 366, 567, 768, 2086, 3263, 3591
HpyF10VI	GCNNNNNNNGC	1 cut(s)	2978
HpyF3I	CTNAG	8 cut(s)	308, 1375, 1596, 2221, 2792, 2802, 2831, 3333
HpySE526I	ACGT	7 cut(s)	168, 366, 567, 768, 2086, 3263, 3591
HspAI	GCGC	2 cut(s)	9, 2560
Kpn2I	TCCGGA	2 cut(s)	1423, 1855
KpnI	GGTACC	1 cut(s)	2243
Ksp22I	TGATCA	1 cut(s)	1000
KspAI	GTTAAC	1 cut(s)	1473
KspI	CCGCGG	1 cut(s)	20
LguI	GCTCTTC	2 cut(s)	3052, 3660
LmnI	GCTCC	5 cut(s)	1460, 2395, 2476, 2924, 3652
Lsp1109I	GCAGC	7 cut(s)	301, 499, 700, 901, 1078, 2062, 3664
MabI	ACCWGGT	1 cut(s)	148
MaeI	CTAG	5 cut(s)	105, 1020, 2202, 2450, 3437
MaeII	ACGT	7 cut(s)	168, 366, 567, 768, 2086, 3263, 3591
MaeIII	GTNAC	8 cut(s)	962, 1229, 1253, 1710, 2775, 3259, 3484, 3645
MbiI	CCGCTC	1 cut(s)	3309
MfeI	CAATTG	5 cut(s)	270, 468, 669, 870, 1326
MflI	RGATCY	5 cut(s)	1054, 1864, 2047, 2702, 3311
MhlI	GDGCHC	4 cut(s)	1043, 1465, 1657, 3479
MlsI	TGGCCA	1 cut(s)	1803
MluNI	TGGCCA	1 cut(s)	1803
MmeI	TCCRAC	5 cut(s)	169, 2107, 2194, 3362, 3505
Mox20I	TGGCCA	1 cut(s)	1803
Mph1103I	ATGCAT	1 cut(s)	3124
MroI	TCCGGA	2 cut(s)	1423, 1855
MroXI	GAANNNNTTC	1 cut(s)	2841
MscI	TGGCCA	1 cut(s)	1803
MseI	TTAA	5 cut(s)	1472, 1926, 2235, 2520, 2579
MslI	CAYNNNNRTG	5 cut(s)	1008, 1403, 2190, 2919, 3266
Msp20I	TGGCCA	1 cut(s)	1803
MspA1I	CMGCKG	1 cut(s)	19
MspI	CCGG	8 cut(s)	65, 71, 87, 1037, 1058, 1424, 1769, 1856
MunI	CAATTG	5 cut(s)	270, 468, 669, 870, 1326
MvaI	CCWGG	6 cut(s)	150, 2039, 2064, 2190, 2825, 3210
MvnI	CGCG	2 cut(s)	19, 111
MwoI	GCNNNNNNNGC	1 cut(s)	2978
NciI	CCSGG	4 cut(s)	65, 71, 1037, 1038
NdeI	CATATG	1 cut(s)	2419
NmuCI	GTSAC	4 cut(s)	1229, 1710, 3259, 3645
NsiI	ATGCAT	1 cut(s)	3124
NspI	RCATGY	2 cut(s)	1737, 3524
NspV	TTCGAA	8 cut(s)	128, 155, 353, 554, 755, 2023, 2892, 3050
OliI	CACNNNNGTG	1 cut(s)	3266
PagI	TCATGA	7 cut(s)	331, 529, 730, 931, 1117, 1605, 3340
PceI	AGGCCT	2 cut(s)	27, 2823
PciSI	GCTCTTC	2 cut(s)	3052, 3660
PcsI	WCGNNNNNNNCGW	3 cut(s)	36, 45, 54
PdmI	GAANNNNTTC	1 cut(s)	2841
PfeI	GAWTC	4 cut(s)	1028, 1972, 2261, 2760
PflFI	GACNNNGTC	3 cut(s)	71, 956, 3257
PfoI	TCCNGGA	1 cut(s)	63
PinAI	ACCGGT	1 cut(s)	1768
PkrI	GCNGC	9 cut(s)	18, 110, 316, 514, 715, 916, 1093, 2077, 3679
PmaCI	CACGTG	1 cut(s)	3264
PmlI	CACGTG	1 cut(s)	3264
Ppu21I	YACGTR	1 cut(s)	3264
PshAI	GACNNNNGTC	4 cut(s)	327, 525, 726, 927
PshBI	ATTAAT	1 cut(s)	2579
Psp1406I	AACGTT	4 cut(s)	168, 366, 567, 768
Psp6I	CCWGG	6 cut(s)	148, 2037, 2062, 2188, 2823, 3208
PspCI	CACGTG	1 cut(s)	3264
PspFI	CCCAGC	1 cut(s)	1464
PspGI	CCWGG	6 cut(s)	148, 2037, 2062, 2188, 2823, 3208
PspPI	GGNCC	6 cut(s)	67, 115, 994, 3206, 3374, 3505
PstI	CTGCAG	1 cut(s)	2863
PstNI	CAGNNNCTG	3 cut(s)	1178, 1283, 3662
PsuI	RGATCY	5 cut(s)	1054, 1864, 2047, 2702, 3311
PsyI	GACNNNGTC	3 cut(s)	71, 956, 3257
RsaI	GTAC	7 cut(s)	1354, 1456, 1623, 2241, 3234, 3402, 3613
RsaNI	GTAC	7 cut(s)	1353, 1455, 1622, 2240, 3233, 3401, 3612
RseI	CAYNNNNRTG	5 cut(s)	1008, 1403, 2190, 2919, 3266
SacII	CCGCGG	1 cut(s)	20
SalI	GTCGAC	1 cut(s)	47
SapI	GCTCTTC	2 cut(s)	3052, 3660
SaqAI	TTAA	5 cut(s)	1472, 1926, 2235, 2520, 2579
SatI	GCNGC	9 cut(s)	17, 109, 315, 513, 714, 915, 1092, 2076, 3678
Sau96I	GGNCC	6 cut(s)	67, 115, 994, 3206, 3374, 3505
SduI	GDGCHC	4 cut(s)	1043, 1465, 1657, 3479
SexAI	ACCWGGT	1 cut(s)	148
SfcI	CTRYAG	2 cut(s)	2715, 2859
Sfr303I	CCGCGG	1 cut(s)	20
SfuI	TTCGAA	8 cut(s)	128, 155, 353, 554, 755, 2023, 2892, 3050
SgrBI	CCGCGG	1 cut(s)	20
SgrDI	CGTCGACG	1 cut(s)	47
SinI	GGWCC	6 cut(s)	67, 115, 994, 3206, 3374, 3505
SmaI	CCCGGG	1 cut(s)	1038
SmiMI	CAYNNNNRTG	5 cut(s)	1008, 1403, 2190, 2919, 3266
SmlI	CTYRAG	2 cut(s)	2555, 2745
SmoI	CTYRAG	2 cut(s)	2555, 2745
SrfI	GCCCGGGC	1 cut(s)	1038
SseBI	AGGCCT	2 cut(s)	27, 2823
SsiI	CCGC	9 cut(s)	14, 17, 19, 109, 1033, 1182, 1787, 2380, 3309
SspI	AATATT	1 cut(s)	1751
SspMI	CTAG	5 cut(s)	105, 1020, 2202, 2450, 3437
StuI	AGGCCT	2 cut(s)	27, 2823
TaiI	ACGT	7 cut(s)	171, 369, 570, 771, 2089, 3266, 3594
TaqII	GACCGA	1 cut(s)	3147
TatI	WGTACW	4 cut(s)	1454, 1621, 3232, 3400
TauI	GCSGC	2 cut(s)	19, 111
TfiI	GAWTC	4 cut(s)	1028, 1972, 2261, 2760
Tru1I	TTAA	5 cut(s)	1472, 1926, 2235, 2520, 2579
Tru9I	TTAA	5 cut(s)	1472, 1926, 2235, 2520, 2579
TseFI	GTSAC	4 cut(s)	1229, 1710, 3259, 3645
TseI	GCWGC	7 cut(s)	314, 512, 713, 914, 1091, 2075, 3677
Tsp45I	GTSAC	4 cut(s)	1229, 1710, 3259, 3645
TspGWI	ACGGA	1 cut(s)	2772
TspMI	CCCGGG	1 cut(s)	1036
Tth111I	GACNNNGTC	3 cut(s)	71, 956, 3257
VneI	GTGCAC	1 cut(s)	1653
VpaK11BI	GGWCC	6 cut(s)	67, 115, 994, 3206, 3374, 3505
VspI	ATTAAT	1 cut(s)	2579
XagI	CCTNNNNNAGG	3 cut(s)	399, 600, 2042
XapI	RAATTY	7 cut(s)	80, 124, 1106, 1537, 1822, 2837, 3564
XbaI	TCTAGA	1 cut(s)	2449
XceI	RCATGY	2 cut(s)	1737, 3524
XmaI	CCCGGG	1 cut(s)	1036
XmiI	GTMKAC	7 cut(s)	36, 48, 266, 464, 665, 866, 3011
XmnI	GAANNNNTTC	1 cut(s)	2841
XspI	CTAG	5 cut(s)	105, 1020, 2202, 2450, 3437
Zsp2I	ATGCAT	1 cut(s)	3124

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.