Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0002625

Physical Location & Seq

Genomic Coordinates Reverse (-)

29962435 .. 29967594

Gene Structure 5160 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1233000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1326 bp

ATGTCGAAGTTTGAGTACCCGAAGCTTACTCGCTCCGACATCGTCACAATCCTCGCCGACGCTCACATCGTCGCTATTTCCGACCGCGATCTCGTCAACCCCAACCCTGATTTCGTCGCCGACCTCTACACCCGCATCCTCGTCTCCCTCGACTTCTTCCACGAGGAAGATTTTGGGCAGGTTGAGTTTTCGGCCTTGGAGCAGCTCGAGAACCCTGATTTTCACATGGATTCGGTCCGGACCATGAAGCTATACAACAGAATCAAGGAAGTGGTGGCTTTAGTGGATTGTCCGAAGAGATTTACCTTAAAAGATCTCGTAAAACCTGAGACGGATCGGACTGAATTTTTTGTCAGTGCACTTCTCAATTTCTCTCTTCACAGAGAGACAAAAATGAATATCCTTACCCAAGTCGTGGATCAATTAACTGACATAGATGAGCGACGCAAAGGATGGGAGGATAAGATTTCCCAGTTGAATGCAGAGATTGCGGACTACAATGAAGCAAGAGAAAAGGAGTTGCCTCTTGTTCAAGAGGTAGATGCAAAAGTTAAAGAATTGCACCACACCGTTTCAGGCCTTAACAATCAGCAAAAGTCACTGAGAACTTCTCGTCAGAAGTTGAAGGAGAAGATCGGAGAAATAGAGGAAAAGGTTTCGAGTGCAGAGTTTTCTCTGGTACAAAGTGTCCAAGAAAATGCAAATTTGCGCTCAAGAATTGTTCAGTCACCAGATAAATTGCAGAGGGCTTTAGAAGAGAAGAAATCAGTTCGTGAGGAGGCGAAAAATGCTGAAAGGTCAGCGAAGCAATCTTTTGAGGAGAAGACTGCTGTTGATGAGGTTTATACAAAGGTCTCTAAGAAATTGTCAAAGCACTTGGCCCAAATGCAGGCTATACAGGAACAGGTCAATTCTGCTAAATCAGTCGACAGAGACGTTAAGGCTGTAAAAGCTAAACTAAGTGATGATGGAGTTGTAAGCAAGTCTCTTCAGGCCAAACTGGTTGAACGGGAGGGAAAAGTGGAACAGTTGAATGAACTAAAAAAGCAGCTAGAAAGAGAGAGAGATCTAAAGTTTGAGGAGGCTTCTAAAGATTTGAATAATGTGGAGCTGGAGGTGGAATCTAGGAGGCGTGATCTAGAAGCAAGGCATAAAGCTGTTGAAGCTGCAGTGGAAGAGGTGGATTCTATCACTTCAAAGACAGCATCGATAAAAGAATCTGGAGCAGCTGATCAGAAAGAATTAGCTCGTAAATGTGAGGAGATCATGAAAGAGTTCCACCAGTACCAGAACTCAATTAGGGTCCTGCTGCTGGGGAGCCAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000070 GO:0000228 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000777 GO:0000778 GO:0000779 GO:0000780 GO:0000793 GO:0000794 GO:0000819 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0005773 GO:0005774 GO:0005815 GO:0005816 GO:0005856 GO:0005886 GO:0006996 GO:0007049 GO:0007059 GO:0007080 GO:0007127 GO:0008150 GO:0008608 GO:0009987 GO:0015630 GO:0016020 GO:0016043 GO:0022402 GO:0022414 GO:0031090 GO:0031262 GO:0031617 GO:0031974 GO:0031981 GO:0032991 GO:0035974 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044437 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0045132 GO:0045143 GO:0048285 GO:0050000 GO:0051179 GO:0051234 GO:0051276 GO:0051303 GO:0051310 GO:0051315 GO:0051316 GO:0051321 GO:0051455 GO:0051640 GO:0051641 GO:0051649 GO:0051656 GO:0061982 GO:0070013 GO:0071840 GO:0071944 GO:0098588 GO:0098687 GO:0098805 GO:0098813 GO:0140013 GO:0140014 GO:1903046 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Nuf2

Protein Analysis

442

Amino Acids

50.62

Weight (kDa)

5.65

Isoelectric Point (pI)

37.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Nuf2	PF03800	4 - 141	3.9e-29	Nuf2 family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002625)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G61000
fragaria_vesca	FvH4_6g25020 FvH4_6g25020 FvH4_6g25020 FvH4_6g25020 FvH4_6g25020 FvH4_6g25020 FvH4_7g06682 FvH4_7g06682 FvH4_7g06682 FvH4_7g06682 FvH4_7g06682
malus_domestica	MD08G1232500.v1.1 MD08G1233000.v1.1 MD15G1425300.v1.1
prunus_persica	Prupe.1G570900_v2.0.a1 Prupe.1G570900_v2.0.a1
pyrus_communis	pycom08g20190 pycom15g37700
rosa_chinensis	RchiOBHm_Chr1g0336301 RchiOBHm_Chr7g0222021
rosa_laevigata	RLG00000002102 RLG00000029374
rosa_multiflora	Rmu_co8043290.1_g000001 Rmu_sc0001453.1_g000009 Rmu_sc0004325.1_g000003
rosa_roxburghii	Rroxscaffold_3G00237490 Rroxscaffold_4G00315800
rosa_rugosa	Rorug01G0123600 Rorug07G0205100
rosa_samantha	Rh1AG144300 Rh1BG113700 Rh1CG135800 Rh1DG150800 Rh7AG345600 Rh7BG336200 Rh7CG363300 Rh7DG343900
rosa_wichuraiana	Rw0G015220 Rw7G029310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	169
AccB7I	CCANNNNNTGG	1 cut(s)	415
AccI	GTMKAC	1 cut(s)	927
AccII	CGCG	1 cut(s)	87
AccIII	TCCGGA	1 cut(s)	237
AciI	CCGC	3 cut(s)	85, 133, 491
AclWI	GGATC	2 cut(s)	342, 426
AcsI	RAATTY	2 cut(s)	344, 703
AcuI	CTGAAG	1 cut(s)	974
AfaI	GTAC	3 cut(s)	17, 681, 1286
AfiI	CCNNNNNNNGG	3 cut(s)	415, 889, 1312
AgsI	TTSAA	8 cut(s)	478, 533, 625, 1007, 1033, 1099, 1163, 1197
AjuI	GAANNNNNNNTTGG	2 cut(s)	95, 127
Alw21I	GWGCWC	1 cut(s)	361
Alw26I	GTCTC	6 cut(s)	148, 323, 380, 859, 927, 990
Alw44I	GTGCAC	1 cut(s)	357
AlwI	GGATC	2 cut(s)	342, 426
Ama87I	CYCGRG	1 cut(s)	206
Aor13HI	TCCGGA	1 cut(s)	237
AoxI	GGCC	4 cut(s)	192, 577, 879, 993
ApaLI	GTGCAC	1 cut(s)	357
ApeKI	GCWGC	5 cut(s)	202, 1048, 1166, 1226, 1309
ApoI	RAATTY	2 cut(s)	344, 703
Asp700I	GAANNNNTTC	1 cut(s)	1274
AspLEI	GCGC	1 cut(s)	711
AspS9I	GGNCC	4 cut(s)	235, 240, 880, 1303
AsuHPI	GGTGA	1 cut(s)	720
AvaI	CYCGRG	1 cut(s)	206
AvaII	GGWCC	3 cut(s)	235, 240, 1303
BaeGI	GKGCMC	1 cut(s)	361
BarI	GAAGNNNNNNTAC	1 cut(s)	31
BauI	CACGAG	1 cut(s)	161
BbsI	GAAGAC	1 cut(s)	830
Bbv12I	GWGCWC	1 cut(s)	361
BbvI	GCAGC	5 cut(s)	214, 1060, 1153, 1238, 1296
BccI	CCATC	2 cut(s)	447, 962
BclI	TGATCA	1 cut(s)	1231
BcoDI	GTCTC	6 cut(s)	148, 323, 380, 859, 927, 990
BfaI	CTAG	3 cut(s)	1052, 1125, 1139
BfmI	CTRYAG	1 cut(s)	1167
BfuAI	ACCTGC	1 cut(s)	169
BglII	AGATCT	2 cut(s)	313, 1066
BisI	GCNGC	5 cut(s)	203, 1049, 1167, 1227, 1310
BlsI	GCNGC	5 cut(s)	204, 1050, 1168, 1228, 1311
Bme18I	GGWCC	3 cut(s)	235, 240, 1303
BmeT110I	CYCGRG	1 cut(s)	206
BmgT120I	GGNCC	4 cut(s)	235, 240, 880, 1303
BmiI	GGNNCC	2 cut(s)	1304, 1319
BmrI	ACTGGG	1 cut(s)	466
BmsI	GCATC	3 cut(s)	144, 532, 1214
BmuI	ACTGGG	1 cut(s)	466
BpiI	GAAGAC	1 cut(s)	830
BplI	GAGNNNNNCTC	2 cut(s)	595, 627
BpmI	CTGGAG	2 cut(s)	1133, 1242
BpuEI	CTTGAG	1 cut(s)	697
Bsa29I	ATCGAT	1 cut(s)	1208
BsaI	GGTCTC	1 cut(s)	859
BsaJI	CCNNGG	1 cut(s)	195
BsaWI	WCCGGW	1 cut(s)	237
Bsc4I	CCNNNNNNNGG	3 cut(s)	415, 889, 1312
Bse1I	ACTGG	3 cut(s)	472, 1005, 1282
BseAI	TCCGGA	1 cut(s)	237
BseCI	ATCGAT	1 cut(s)	1208
BseDI	CCNNGG	1 cut(s)	195
BseGI	GGATG	2 cut(s)	135, 458
BseLI	CCNNNNNNNGG	3 cut(s)	415, 889, 1312
BseMII	CTCAG	2 cut(s)	318, 593
BseNI	ACTGG	3 cut(s)	472, 1005, 1282
BseRI	GAGGAG	4 cut(s)	791, 833, 1094, 1274
BseSI	GKGCMC	1 cut(s)	361
BseXI	GCAGC	5 cut(s)	214, 1060, 1153, 1238, 1296
BseYI	CCCAGC	1 cut(s)	1312
BsgI	GTGCAG	1 cut(s)	684
Bsh1236I	CGCG	1 cut(s)	87
Bsh1285I	CGRYCG	1 cut(s)	85
BshFI	GGCC	4 cut(s)	194, 579, 881, 995
BshVI	ATCGAT	1 cut(s)	1208
BsiEI	CGRYCG	1 cut(s)	85
BsiHKAI	GWGCWC	1 cut(s)	361
BsiHKCI	CYCGRG	1 cut(s)	206
BsiSI	CCGG	1 cut(s)	238
BslI	CCNNNNNNNGG	3 cut(s)	415, 889, 1312
BsmAI	GTCTC	6 cut(s)	148, 323, 380, 859, 927, 990
BsmBI	CGTCTC	3 cut(s)	148, 323, 927
BsmI	GAATGC	1 cut(s)	484
BsnI	GGCC	4 cut(s)	194, 579, 881, 995
Bso31I	GGTCTC	1 cut(s)	859
BsoBI	CYCGRG	1 cut(s)	206
Bsp1286I	GDGCHC	1 cut(s)	361
Bsp13I	TCCGGA	1 cut(s)	237
Bsp143I	GATC	9 cut(s)	88, 313, 334, 418, 633, 1066, 1135, 1231, 1263
BspACI	CCGC	3 cut(s)	85, 133, 491
BspANI	GGCC	4 cut(s)	194, 579, 881, 995
BspCNI	CTCAG	2 cut(s)	319, 594
BspDI	ATCGAT	1 cut(s)	1208
BspEI	TCCGGA	1 cut(s)	237
BspFNI	CGCG	1 cut(s)	87
BspHI	TCATGA	1 cut(s)	1266
BspLI	GGNNCC	2 cut(s)	1304, 1319
BspMAI	CTGCAG	1 cut(s)	1171
BspMI	ACCTGC	1 cut(s)	169
BspPI	GGATC	2 cut(s)	342, 426
BspTNI	GGTCTC	1 cut(s)	859
BsrI	ACTGG	3 cut(s)	472, 1005, 1282
BssECI	CCNNGG	1 cut(s)	195
BssMI	GATC	9 cut(s)	88, 313, 334, 418, 633, 1066, 1135, 1231, 1263
BssSI	CACGAG	1 cut(s)	161
BssT1I	CCWWGG	1 cut(s)	195
Bst2BI	CACGAG	1 cut(s)	161
Bst4CI	ACNGT	2 cut(s)	571, 1029
Bst6I	CTCTTC	5 cut(s)	290, 381, 750, 993, 1170
BstAPI	GCANNNNNTGC	1 cut(s)	488
BstC8I	GCNNGC	1 cut(s)	891
BstDEI	CTNAG	4 cut(s)	327, 602, 858, 959
BstF5I	GGATG	2 cut(s)	135, 458
BstFNI	CGCG	1 cut(s)	87
BstHHI	GCGC	1 cut(s)	711
BstKTI	GATC	9 cut(s)	91, 316, 337, 421, 636, 1069, 1138, 1234, 1266
BstMAI	GTCTC	6 cut(s)	148, 323, 380, 859, 927, 990
BstMBI	GATC	9 cut(s)	88, 313, 334, 418, 633, 1066, 1135, 1231, 1263
BstMCI	CGRYCG	1 cut(s)	85
BstMWI	GCNNNNNNNGC	4 cut(s)	488, 788, 950, 1163
BstSFI	CTRYAG	1 cut(s)	1167
BstSLI	GKGCMC	1 cut(s)	361
BstUI	CGCG	1 cut(s)	87
BstV1I	GCAGC	5 cut(s)	214, 1060, 1153, 1238, 1296
BstV2I	GAAGAC	1 cut(s)	830
BstX2I	RGATCY	2 cut(s)	313, 1066
BstYI	RGATCY	2 cut(s)	313, 1066
Bsu15I	ATCGAT	1 cut(s)	1208
BsuRI	GGCC	4 cut(s)	194, 579, 881, 995
BsuTUI	ATCGAT	1 cut(s)	1208
BtsCI	GGATG	2 cut(s)	135, 458
BtsI	GCAGTG	1 cut(s)	1176
BtsIMutI	CAGTG	3 cut(s)	361, 599, 1176
BveI	ACCTGC	1 cut(s)	169
Cac8I	GCNNGC	1 cut(s)	891
CciI	TCATGA	1 cut(s)	1266
CfoI	GCGC	1 cut(s)	711
Cfr13I	GGNCC	4 cut(s)	235, 240, 880, 1303
ClaI	ATCGAT	1 cut(s)	1208
CpoI	CGGWCCG	1 cut(s)	235
CseI	GACGC	2 cut(s)	68, 453
Csp6I	GTAC	3 cut(s)	16, 680, 1285
CspI	CGGWCCG	1 cut(s)	235
CviAII	CATG	3 cut(s)	226, 244, 1267
CviQI	GTAC	3 cut(s)	16, 680, 1285
DdeI	CTNAG	4 cut(s)	327, 602, 858, 959
DpnI	GATC	9 cut(s)	90, 315, 336, 420, 635, 1068, 1137, 1233, 1265
DpnII	GATC	9 cut(s)	88, 313, 334, 418, 633, 1066, 1135, 1231, 1263
Eam1104I	CTCTTC	5 cut(s)	290, 381, 750, 993, 1170
EarI	CTCTTC	5 cut(s)	290, 381, 750, 993, 1170
Eco130I	CCWWGG	1 cut(s)	195
Eco147I	AGGCCT	1 cut(s)	579
Eco31I	GGTCTC	1 cut(s)	859
Eco47I	GGWCC	3 cut(s)	235, 240, 1303
Eco57I	CTGAAG	1 cut(s)	974
Eco88I	CYCGRG	1 cut(s)	206
EcoO109I	RGGNCCY	1 cut(s)	1303
EcoT14I	CCWWGG	1 cut(s)	195
ErhI	CCWWGG	1 cut(s)	195
Esp3I	CGTCTC	3 cut(s)	148, 323, 927
FaeI	CATG	3 cut(s)	229, 247, 1270
FaiI	YATR	8 cut(s)	227, 245, 253, 434, 846, 896, 1152, 1268
FatI	CATG	3 cut(s)	225, 243, 1266
FauI	CCCGC	1 cut(s)	140
FbaI	TGATCA	1 cut(s)	1231
FblI	GTMKAC	1 cut(s)	927
Fnu4HI	GCNGC	5 cut(s)	203, 1049, 1167, 1227, 1310
FokI	GGATG	2 cut(s)	122, 465
Fsp4HI	GCNGC	5 cut(s)	203, 1049, 1167, 1227, 1310
FspBI	CTAG	3 cut(s)	1052, 1125, 1139
GlaI	GCGC	1 cut(s)	710
GluI	GCNGC	5 cut(s)	203, 1049, 1167, 1227, 1310
GsaI	CCCAGC	1 cut(s)	1316
GsuI	CTGGAG	2 cut(s)	1133, 1242
HaeIII	GGCC	4 cut(s)	194, 579, 881, 995
HapII	CCGG	1 cut(s)	238
HgaI	GACGC	2 cut(s)	68, 453
HhaI	GCGC	1 cut(s)	711
Hin1II	CATG	3 cut(s)	229, 247, 1270
Hin6I	GCGC	1 cut(s)	709
HinP1I	GCGC	1 cut(s)	709
HincII	GTYRAC	2 cut(s)	97, 928
HindII	GTYRAC	2 cut(s)	97, 928
HindIII	AAGCTT	1 cut(s)	23
HinfI	GANTC	5 cut(s)	230, 261, 1121, 1184, 1217
HpaII	CCGG	1 cut(s)	238
HphI	GGTGA	1 cut(s)	720
Hpy166II	GTNNAC	3 cut(s)	97, 359, 928
Hpy188I	TCNGA	7 cut(s)	37, 82, 294, 339, 618, 638, 1236
Hpy188III	TCNNGA	8 cut(s)	208, 238, 533, 714, 773, 1139, 1221, 1267
Hpy8I	GTNNAC	3 cut(s)	97, 359, 928
Hpy99I	CGWCG	4 cut(s)	62, 74, 119, 447
HpyAV	CCTTC	1 cut(s)	619
HpyCH4III	ACNGT	2 cut(s)	571, 1029
HpyCH4IV	ACGT	1 cut(s)	936
HpyCH4V	TGCA	9 cut(s)	359, 482, 545, 562, 665, 701, 742, 889, 1169
HpyF10VI	GCNNNNNNNGC	4 cut(s)	488, 788, 950, 1163
HpyF3I	CTNAG	4 cut(s)	327, 602, 858, 959
HpySE526I	ACGT	1 cut(s)	936
Hsp92II	CATG	3 cut(s)	229, 247, 1270
HspAI	GCGC	1 cut(s)	709
Kpn2I	TCCGGA	1 cut(s)	237
Ksp22I	TGATCA	1 cut(s)	1231
Kzo9I	GATC	9 cut(s)	88, 313, 334, 418, 633, 1066, 1135, 1231, 1263
LmnI	GCTCC	5 cut(s)	38, 199, 1108, 1223, 1317
Lsp1109I	GCAGC	5 cut(s)	214, 1060, 1153, 1238, 1296
LweI	GCATC	3 cut(s)	144, 532, 1214
MaeI	CTAG	3 cut(s)	1052, 1125, 1139
MaeII	ACGT	1 cut(s)	936
MaeIII	GTNAC	3 cut(s)	43, 597, 726
MalI	GATC	9 cut(s)	90, 315, 336, 420, 635, 1068, 1137, 1233, 1265
MboI	GATC	9 cut(s)	88, 313, 334, 418, 633, 1066, 1135, 1231, 1263
MflI	RGATCY	2 cut(s)	313, 1066
MhlI	GDGCHC	1 cut(s)	361
MmeI	TCCRAC	2 cut(s)	60, 105
MroI	TCCGGA	1 cut(s)	237
MroXI	GAANNNNTTC	1 cut(s)	1274
MseI	TTAA	5 cut(s)	308, 425, 552, 582, 939
MspA1I	CMGCKG	1 cut(s)	1229
MspI	CCGG	1 cut(s)	238
Mva1269I	GAATGC	1 cut(s)	484
MvnI	CGCG	1 cut(s)	87
MwoI	GCNNNNNNNGC	4 cut(s)	488, 788, 950, 1163
NdeII	GATC	9 cut(s)	88, 313, 334, 418, 633, 1066, 1135, 1231, 1263
NlaIII	CATG	3 cut(s)	229, 247, 1270
NlaIV	GGNNCC	2 cut(s)	1304, 1319
NmuCI	GTSAC	3 cut(s)	43, 597, 726
PaeR7I	CTCGAG	1 cut(s)	206
PagI	TCATGA	1 cut(s)	1266
PceI	AGGCCT	1 cut(s)	579
PcsI	WCGNNNNNNNCGW	4 cut(s)	66, 78, 147, 933
PctI	GAATGC	1 cut(s)	484
PdmI	GAANNNNTTC	1 cut(s)	1274
PfeI	GAWTC	5 cut(s)	230, 261, 1121, 1184, 1217
PflFI	GACNNNGTC	1 cut(s)	41
PflMI	CCANNNNNTGG	1 cut(s)	415
PkrI	GCNGC	5 cut(s)	204, 1050, 1168, 1228, 1311
PpuMI	RGGWCCY	1 cut(s)	1303
Psp5II	RGGWCCY	1 cut(s)	1303
PspFI	CCCAGC	1 cut(s)	1312
PspN4I	GGNNCC	2 cut(s)	1304, 1319
PspPI	GGNCC	4 cut(s)	235, 240, 880, 1303
PspPPI	RGGWCCY	1 cut(s)	1303
PstI	CTGCAG	1 cut(s)	1171
PsuI	RGATCY	2 cut(s)	313, 1066
PsyI	GACNNNGTC	1 cut(s)	41
PvuII	CAGCTG	1 cut(s)	1229
RsaI	GTAC	3 cut(s)	17, 681, 1286
RsaNI	GTAC	3 cut(s)	16, 680, 1285
Rsr2I	CGGWCCG	1 cut(s)	235
RsrII	CGGWCCG	1 cut(s)	235
SalI	GTCGAC	1 cut(s)	926
SaqAI	TTAA	5 cut(s)	308, 425, 552, 582, 939
SatI	GCNGC	5 cut(s)	203, 1049, 1167, 1227, 1310
Sau3AI	GATC	9 cut(s)	88, 313, 334, 418, 633, 1066, 1135, 1231, 1263
Sau96I	GGNCC	4 cut(s)	235, 240, 880, 1303
SduI	GDGCHC	1 cut(s)	361
SfaNI	GCATC	3 cut(s)	144, 532, 1214
SfcI	CTRYAG	1 cut(s)	1167
Sfr274I	CTCGAG	1 cut(s)	206
SinI	GGWCC	3 cut(s)	235, 240, 1303
SlaI	CTCGAG	1 cut(s)	206
SmlI	CTYRAG	2 cut(s)	206, 712
SmoI	CTYRAG	2 cut(s)	206, 712
SseBI	AGGCCT	1 cut(s)	579
SsiI	CCGC	3 cut(s)	85, 133, 491
SspMI	CTAG	3 cut(s)	1052, 1125, 1139
StuI	AGGCCT	1 cut(s)	579
StyI	CCWWGG	1 cut(s)	195
TaaI	ACNGT	2 cut(s)	571, 1029
TaiI	ACGT	1 cut(s)	939
TaqI	TCGA	6 cut(s)	5, 150, 207, 659, 927, 1208
TaqII	GACCGA	1 cut(s)	223
TfiI	GAWTC	5 cut(s)	230, 261, 1121, 1184, 1217
Tru1I	TTAA	5 cut(s)	308, 425, 552, 582, 939
Tru9I	TTAA	5 cut(s)	308, 425, 552, 582, 939
TscAI	CASTG	3 cut(s)	361, 606, 1176
TseFI	GTSAC	3 cut(s)	43, 597, 726
TseI	GCWGC	5 cut(s)	202, 1048, 1166, 1226, 1309
Tsp45I	GTSAC	3 cut(s)	43, 597, 726
TspDTI	ATGAA	5 cut(s)	260, 410, 516, 1050, 1283
TspGWI	ACGGA	1 cut(s)	347
TspRI	CASTG	3 cut(s)	361, 606, 1176
Tth111I	GACNNNGTC	1 cut(s)	41
Van91I	CCANNNNNTGG	1 cut(s)	415
VneI	GTGCAC	1 cut(s)	357
VpaK11BI	GGWCC	3 cut(s)	235, 240, 1303
XapI	RAATTY	2 cut(s)	344, 703
XbaI	TCTAGA	1 cut(s)	1138
XhoI	CTCGAG	1 cut(s)	206
XmiI	GTMKAC	1 cut(s)	927
XmnI	GAANNNNTTC	1 cut(s)	1274
XspI	CTAG	3 cut(s)	1052, 1125, 1139

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.