Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0002625

Physical Location & Seq

Genomic Coordinates Reverse (-)

52624254 .. 52629642

Gene Structure 5389 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1425300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1326 bp

ATGTCGAAGTTTGAGTACCCGAAGCTTACTCGCTCCGACATCGTCACAATCCTCGCCGACTCTCACATCGTCGTTATTTCCGACCGCGATCTCGTCAACCCCAATCCTGATTTCGTCGCCGACCTCTACACCCGCATCCTCATCTCCCTCGACTTCTTCCACGAGGAAGATTATGGGCAGGTTGAGTTTTCGGCCTTGGAGCAGCTCGAGAACCCGGATTTTCACATGGACTCGGTCCGGACCATGAAGCTATACAACAGAATCAAGGAAGTGGTGGCTTTAGTGGATTGTCCTAAAAGATTTACTCTAAAAGATATGATAAAACCCGAGACGGATCGGACTGAATATTTTGTCAGTGCACTTCTCAATTTCTCTCTTCACAGAGAGACAAAAATGAATACCCTTACCCAAGTCGTGGATCAATTAACTGACATAGATGAGCAACGCAAAGGGTGGGAGGACAAAATTTCCCAGTTGAATGCAGAGATTGCGGACTACAATGAAGCAAGAGAAAAGGAGTTGCCTCTTGTTCAAGAGGTAGATGCAAAAGTTAAAGAATTGCACCAAACTGTTTCAGGCCTTAACAATCAGCAAAAGTCACTGAGAACTTCTCGTCAGAAGTTGAAGGAGAAGATTGGAGAAATAGAGGAAAAGGTTTCGAGTGCAGAGTTTTCTTTGGTACAAAGTGTTCAAGAAAATGCAAATTTGCGCTCAAAAATTGTTCAGTCACCAGATAAATTGCAGAGGGCTTTAGAAGAGAAGAAATCAGTTCGTGAGGAGGCGAAAAATGCTGAAAGGTCAGCGAAGCAATCCTTGGAGGAGAAGACCGCTGTTGATGAGGTTTATACAAAGGTGTCTAAGAAATTGTCAAAACACTTGGCCCAAATGCAAGCTATACAAGAACAGGTTAATTCTGCTAAATCAGTCGACAGAGACTTTAAGGCTGTAAAAGCTAAACTAAGTGATGATGGAGTTGTAAGCAAGTCTCTTCAGGCCAAACTGGTTGAACGGGAGGGAAAAGTGGAACAGTTGAATGAATTAAAAAAGAAGCTAGAAAGGGAAAGAGATTTGAAGTTTGAGGAGGCTTCTAAAGATTTGAATAATGTGGAGCTGGAGGTGGAATCTAGGAGGCGTGATCTAGAAGCAAGGCAAAAAGCTGTTGAAGCTGCGGTGGAAGAGGTGGATTCTATCACTTCAAAGACAGTATCAATAAAAGAATCTGGGGCAGCTGATCAGAAAGAATTAGCTCGTAAATGCGAGGAGATTATGAAAGAGTTCCACCAGTACCAGAACTCAATCAGGGTCCTGCTGATGGAGAGCCAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000070 GO:0000228 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000777 GO:0000778 GO:0000779 GO:0000780 GO:0000793 GO:0000794 GO:0000819 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0005773 GO:0005774 GO:0005815 GO:0005816 GO:0005856 GO:0005886 GO:0006996 GO:0007049 GO:0007059 GO:0007080 GO:0007127 GO:0008150 GO:0008608 GO:0009987 GO:0015630 GO:0016020 GO:0016043 GO:0022402 GO:0022414 GO:0031090 GO:0031262 GO:0031617 GO:0031974 GO:0031981 GO:0032991 GO:0035974 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044437 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0045132 GO:0045143 GO:0048285 GO:0050000 GO:0051179 GO:0051234 GO:0051276 GO:0051303 GO:0051310 GO:0051315 GO:0051316 GO:0051321 GO:0051455 GO:0051640 GO:0051641 GO:0051649 GO:0051656 GO:0061982 GO:0070013 GO:0071840 GO:0071944 GO:0098588 GO:0098687 GO:0098805 GO:0098813 GO:0140013 GO:0140014 GO:1903046 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Nuf2

Protein Analysis

442

Amino Acids

50.79

Weight (kDa)

5.48

Isoelectric Point (pI)

33.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Nuf2	PF03800	4 - 141	2.3e-28	Nuf2 family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002625)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G61000
fragaria_vesca	FvH4_6g25020 FvH4_6g25020 FvH4_6g25020 FvH4_6g25020 FvH4_6g25020 FvH4_6g25020 FvH4_7g06682 FvH4_7g06682 FvH4_7g06682 FvH4_7g06682 FvH4_7g06682
malus_domestica	MD08G1232500.v1.1 MD08G1233000.v1.1 MD15G1425300.v1.1
prunus_persica	Prupe.1G570900_v2.0.a1 Prupe.1G570900_v2.0.a1
pyrus_communis	pycom08g20190 pycom15g37700
rosa_chinensis	RchiOBHm_Chr1g0336301 RchiOBHm_Chr7g0222021
rosa_laevigata	RLG00000002102 RLG00000029374
rosa_multiflora	Rmu_co8043290.1_g000001 Rmu_sc0001453.1_g000009 Rmu_sc0004325.1_g000003
rosa_roxburghii	Rroxscaffold_3G00237490 Rroxscaffold_4G00315800
rosa_rugosa	Rorug01G0123600 Rorug07G0205100
rosa_samantha	Rh1AG144300 Rh1BG113700 Rh1CG135800 Rh1DG150800 Rh7AG345600 Rh7BG336200 Rh7CG363300 Rh7DG343900
rosa_wichuraiana	Rw0G015220 Rw7G029310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	169
AccB7I	CCANNNNNTGG	1 cut(s)	415
AccI	GTMKAC	1 cut(s)	927
AccII	CGCG	1 cut(s)	87
AccIII	TCCGGA	1 cut(s)	237
AciI	CCGC	5 cut(s)	85, 133, 491, 828, 1169
AclWI	GGATC	2 cut(s)	342, 426
AcsI	RAATTY	2 cut(s)	465, 703
AcuI	CTGAAG	1 cut(s)	974
AfaI	GTAC	3 cut(s)	17, 681, 1286
AfiI	CCNNNNNNNGG	2 cut(s)	415, 1312
AjuI	GAANNNNNNNTTGG	4 cut(s)	95, 127, 797, 829
Alw21I	GWGCWC	1 cut(s)	361
Alw26I	GTCTC	4 cut(s)	323, 380, 927, 990
Alw44I	GTGCAC	1 cut(s)	357
AlwI	GGATC	2 cut(s)	342, 426
Ama87I	CYCGRG	2 cut(s)	206, 326
Aor13HI	TCCGGA	1 cut(s)	237
AoxI	GGCC	4 cut(s)	192, 577, 879, 993
ApaLI	GTGCAC	1 cut(s)	357
ApeKI	GCWGC	3 cut(s)	202, 1166, 1226
ApoI	RAATTY	2 cut(s)	465, 703
Asp700I	GAANNNNTTC	1 cut(s)	1274
AspLEI	GCGC	1 cut(s)	711
AspS9I	GGNCC	4 cut(s)	235, 240, 880, 1303
AsuC2I	CCSGG	1 cut(s)	215
AsuHPI	GGTGA	1 cut(s)	720
AvaI	CYCGRG	2 cut(s)	206, 326
AvaII	GGWCC	3 cut(s)	235, 240, 1303
BaeGI	GKGCMC	1 cut(s)	361
BarI	GAAGNNNNNNTAC	1 cut(s)	31
BauI	CACGAG	1 cut(s)	161
BbsI	GAAGAC	1 cut(s)	830
Bbv12I	GWGCWC	1 cut(s)	361
BbvI	GCAGC	3 cut(s)	214, 1153, 1238
BccI	CCATC	2 cut(s)	962, 1306
BclI	TGATCA	1 cut(s)	1231
BcnI	CCSGG	1 cut(s)	215
BcoDI	GTCTC	4 cut(s)	323, 380, 927, 990
BfaI	CTAG	3 cut(s)	1052, 1125, 1139
BfuAI	ACCTGC	1 cut(s)	169
BisI	GCNGC	3 cut(s)	203, 1167, 1227
BlsI	GCNGC	3 cut(s)	204, 1168, 1228
Bme1390I	CCNGG	1 cut(s)	215
Bme18I	GGWCC	3 cut(s)	235, 240, 1303
BmeT110I	CYCGRG	2 cut(s)	206, 326
BmgT120I	GGNCC	4 cut(s)	235, 240, 880, 1303
BmiI	GGNNCC	1 cut(s)	1304
BmrFI	CCNGG	1 cut(s)	215
BmrI	ACTGGG	1 cut(s)	466
BmsI	GCATC	2 cut(s)	144, 532
BmuI	ACTGGG	1 cut(s)	466
BpiI	GAAGAC	1 cut(s)	830
BplI	GAGNNNNNCTC	2 cut(s)	595, 627
BpmI	CTGGAG	1 cut(s)	1133
BpuMI	CCSGG	1 cut(s)	215
BsaJI	CCNNGG	2 cut(s)	195, 813
BsaWI	WCCGGW	1 cut(s)	237
Bsc4I	CCNNNNNNNGG	2 cut(s)	415, 1312
Bse1I	ACTGG	3 cut(s)	472, 1005, 1282
BseAI	TCCGGA	1 cut(s)	237
BseDI	CCNNGG	2 cut(s)	195, 813
BseGI	GGATG	1 cut(s)	135
BseLI	CCNNNNNNNGG	2 cut(s)	415, 1312
BseMII	CTCAG	1 cut(s)	593
BseNI	ACTGG	3 cut(s)	472, 1005, 1282
BseRI	GAGGAG	4 cut(s)	791, 833, 1094, 1274
BseSI	GKGCMC	1 cut(s)	361
BseXI	GCAGC	3 cut(s)	214, 1153, 1238
BsgI	GTGCAG	1 cut(s)	684
Bsh1236I	CGCG	1 cut(s)	87
Bsh1285I	CGRYCG	1 cut(s)	85
BshFI	GGCC	4 cut(s)	194, 579, 881, 995
BsiEI	CGRYCG	1 cut(s)	85
BsiHKAI	GWGCWC	1 cut(s)	361
BsiHKCI	CYCGRG	2 cut(s)	206, 326
BsiSI	CCGG	2 cut(s)	215, 238
BslI	CCNNNNNNNGG	2 cut(s)	415, 1312
BsmAI	GTCTC	4 cut(s)	323, 380, 927, 990
BsmBI	CGTCTC	1 cut(s)	323
BsmI	GAATGC	1 cut(s)	484
BsnI	GGCC	4 cut(s)	194, 579, 881, 995
BsoBI	CYCGRG	2 cut(s)	206, 326
Bsp1286I	GDGCHC	1 cut(s)	361
Bsp13I	TCCGGA	1 cut(s)	237
Bsp143I	GATC	5 cut(s)	88, 334, 418, 1135, 1231
BspACI	CCGC	5 cut(s)	85, 133, 491, 828, 1169
BspANI	GGCC	4 cut(s)	194, 579, 881, 995
BspCNI	CTCAG	1 cut(s)	594
BspEI	TCCGGA	1 cut(s)	237
BspFNI	CGCG	1 cut(s)	87
BspLI	GGNNCC	1 cut(s)	1304
BspMI	ACCTGC	1 cut(s)	169
BspPI	GGATC	2 cut(s)	342, 426
BsrI	ACTGG	3 cut(s)	472, 1005, 1282
BssECI	CCNNGG	2 cut(s)	195, 813
BssMI	GATC	5 cut(s)	88, 334, 418, 1135, 1231
BssSI	CACGAG	1 cut(s)	161
BssT1I	CCWWGG	2 cut(s)	195, 813
Bst2BI	CACGAG	1 cut(s)	161
Bst4CI	ACNGT	3 cut(s)	571, 1029, 1204
Bst6I	CTCTTC	4 cut(s)	381, 750, 993, 1170
BstAPI	GCANNNNNTGC	1 cut(s)	488
BstC8I	GCNNGC	1 cut(s)	891
BstDEI	CTNAG	3 cut(s)	602, 858, 959
BstF5I	GGATG	1 cut(s)	135
BstFNI	CGCG	1 cut(s)	87
BstHHI	GCGC	1 cut(s)	711
BstKTI	GATC	5 cut(s)	91, 337, 421, 1138, 1234
BstMAI	GTCTC	4 cut(s)	323, 380, 927, 990
BstMBI	GATC	5 cut(s)	88, 334, 418, 1135, 1231
BstMCI	CGRYCG	1 cut(s)	85
BstMWI	GCNNNNNNNGC	4 cut(s)	488, 788, 950, 1163
BstSCI	CCNGG	1 cut(s)	213
BstSLI	GKGCMC	1 cut(s)	361
BstUI	CGCG	1 cut(s)	87
BstV1I	GCAGC	3 cut(s)	214, 1153, 1238
BstV2I	GAAGAC	1 cut(s)	830
BsuRI	GGCC	4 cut(s)	194, 579, 881, 995
BtsCI	GGATG	1 cut(s)	135
BtsIMutI	CAGTG	2 cut(s)	361, 599
BveI	ACCTGC	1 cut(s)	169
Cac8I	GCNNGC	1 cut(s)	891
CfoI	GCGC	1 cut(s)	711
Cfr13I	GGNCC	4 cut(s)	235, 240, 880, 1303
CpoI	CGGWCCG	1 cut(s)	235
Csp6I	GTAC	3 cut(s)	16, 680, 1285
CspI	CGGWCCG	1 cut(s)	235
CviAII	CATG	2 cut(s)	226, 244
CviQI	GTAC	3 cut(s)	16, 680, 1285
DdeI	CTNAG	3 cut(s)	602, 858, 959
DpnI	GATC	5 cut(s)	90, 336, 420, 1137, 1233
DpnII	GATC	5 cut(s)	88, 334, 418, 1135, 1231
Eam1104I	CTCTTC	4 cut(s)	381, 750, 993, 1170
EarI	CTCTTC	4 cut(s)	381, 750, 993, 1170
Eco130I	CCWWGG	2 cut(s)	195, 813
Eco147I	AGGCCT	1 cut(s)	579
Eco47I	GGWCC	3 cut(s)	235, 240, 1303
Eco57I	CTGAAG	1 cut(s)	974
Eco88I	CYCGRG	2 cut(s)	206, 326
EcoO109I	RGGNCCY	1 cut(s)	1303
EcoT14I	CCWWGG	2 cut(s)	195, 813
ErhI	CCWWGG	2 cut(s)	195, 813
Esp3I	CGTCTC	1 cut(s)	323
FaeI	CATG	2 cut(s)	229, 247
FaiI	YATR	9 cut(s)	174, 227, 245, 253, 317, 434, 846, 896, 1268
FalI	AAGNNNNNCTT	2 cut(s)	797, 829
FatI	CATG	2 cut(s)	225, 243
FauI	CCCGC	1 cut(s)	140
FbaI	TGATCA	1 cut(s)	1231
FblI	GTMKAC	1 cut(s)	927
Fnu4HI	GCNGC	3 cut(s)	203, 1167, 1227
FokI	GGATG	1 cut(s)	122
Fsp4HI	GCNGC	3 cut(s)	203, 1167, 1227
FspBI	CTAG	3 cut(s)	1052, 1125, 1139
GlaI	GCGC	1 cut(s)	710
GluI	GCNGC	3 cut(s)	203, 1167, 1227
GsuI	CTGGAG	1 cut(s)	1133
HaeIII	GGCC	4 cut(s)	194, 579, 881, 995
HapII	CCGG	2 cut(s)	215, 238
HhaI	GCGC	1 cut(s)	711
Hin1II	CATG	2 cut(s)	229, 247
Hin6I	GCGC	1 cut(s)	709
HinP1I	GCGC	1 cut(s)	709
HincII	GTYRAC	2 cut(s)	97, 928
HindII	GTYRAC	2 cut(s)	97, 928
HindIII	AAGCTT	1 cut(s)	23
HinfI	GANTC	6 cut(s)	59, 230, 261, 1121, 1184, 1217
HpaII	CCGG	2 cut(s)	215, 238
HphI	GGTGA	1 cut(s)	720
Hpy166II	GTNNAC	3 cut(s)	97, 359, 928
Hpy188I	TCNGA	5 cut(s)	37, 82, 339, 618, 1236
Hpy188III	TCNNGA	7 cut(s)	107, 208, 238, 533, 692, 773, 1139
Hpy8I	GTNNAC	3 cut(s)	97, 359, 928
Hpy99I	CGWCG	2 cut(s)	74, 119
HpyAV	CCTTC	1 cut(s)	619
HpyCH4III	ACNGT	3 cut(s)	571, 1029, 1204
HpyCH4V	TGCA	8 cut(s)	359, 482, 545, 562, 665, 701, 742, 889
HpyF10VI	GCNNNNNNNGC	4 cut(s)	488, 788, 950, 1163
HpyF3I	CTNAG	3 cut(s)	602, 858, 959
Hsp92II	CATG	2 cut(s)	229, 247
HspAI	GCGC	1 cut(s)	709
Kpn2I	TCCGGA	1 cut(s)	237
Ksp22I	TGATCA	1 cut(s)	1231
Kzo9I	GATC	5 cut(s)	88, 334, 418, 1135, 1231
LmnI	GCTCC	3 cut(s)	38, 199, 1108
Lsp1109I	GCAGC	3 cut(s)	214, 1153, 1238
LweI	GCATC	2 cut(s)	144, 532
MaeI	CTAG	3 cut(s)	1052, 1125, 1139
MaeIII	GTNAC	3 cut(s)	43, 597, 726
MalI	GATC	5 cut(s)	90, 336, 420, 1137, 1233
MboI	GATC	5 cut(s)	88, 334, 418, 1135, 1231
MboII	GAAGA	9 cut(s)	148, 179, 368, 643, 767, 772, 835, 980, 1187
MhlI	GDGCHC	1 cut(s)	361
MlyI	GAGTC	2 cut(s)	53, 224
MmeI	TCCRAC	2 cut(s)	60, 105
MroI	TCCGGA	1 cut(s)	237
MroXI	GAANNNNTTC	1 cut(s)	1274
MseI	TTAA	6 cut(s)	425, 552, 582, 909, 939, 1040
MspA1I	CMGCKG	2 cut(s)	830, 1229
MspI	CCGG	2 cut(s)	215, 238
MspR9I	CCNGG	1 cut(s)	215
Mva1269I	GAATGC	1 cut(s)	484
MvnI	CGCG	1 cut(s)	87
MwoI	GCNNNNNNNGC	4 cut(s)	488, 788, 950, 1163
NciI	CCSGG	1 cut(s)	215
NdeII	GATC	5 cut(s)	88, 334, 418, 1135, 1231
NlaIII	CATG	2 cut(s)	229, 247
NlaIV	GGNNCC	1 cut(s)	1304
NmuCI	GTSAC	3 cut(s)	43, 597, 726
PaeR7I	CTCGAG	1 cut(s)	206
PceI	AGGCCT	1 cut(s)	579
PcsI	WCGNNNNNNNCGW	1 cut(s)	78
PctI	GAATGC	1 cut(s)	484
PdmI	GAANNNNTTC	1 cut(s)	1274
PfeI	GAWTC	4 cut(s)	261, 1121, 1184, 1217
PflFI	GACNNNGTC	2 cut(s)	41, 233
PflMI	CCANNNNNTGG	1 cut(s)	415
PkrI	GCNGC	3 cut(s)	204, 1168, 1228
PleI	GAGTC	2 cut(s)	53, 224
PpsI	GAGTC	2 cut(s)	53, 224
PpuMI	RGGWCCY	1 cut(s)	1303
Psp5II	RGGWCCY	1 cut(s)	1303
PspN4I	GGNNCC	1 cut(s)	1304
PspPI	GGNCC	4 cut(s)	235, 240, 880, 1303
PspPPI	RGGWCCY	1 cut(s)	1303
PsrI	GAACNNNNNNTAC	2 cut(s)	672, 704
PsyI	GACNNNGTC	2 cut(s)	41, 233
PvuII	CAGCTG	1 cut(s)	1229
RsaI	GTAC	3 cut(s)	17, 681, 1286
RsaNI	GTAC	3 cut(s)	16, 680, 1285
Rsr2I	CGGWCCG	1 cut(s)	235
RsrII	CGGWCCG	1 cut(s)	235
SalI	GTCGAC	1 cut(s)	926
SaqAI	TTAA	6 cut(s)	425, 552, 582, 909, 939, 1040
SatI	GCNGC	3 cut(s)	203, 1167, 1227
Sau3AI	GATC	5 cut(s)	88, 334, 418, 1135, 1231
Sau96I	GGNCC	4 cut(s)	235, 240, 880, 1303
SchI	GAGTC	2 cut(s)	53, 224
ScrFI	CCNGG	1 cut(s)	215
SduI	GDGCHC	1 cut(s)	361
SfaNI	GCATC	2 cut(s)	144, 532
Sfr274I	CTCGAG	1 cut(s)	206
SinI	GGWCC	3 cut(s)	235, 240, 1303
SlaI	CTCGAG	1 cut(s)	206
SmlI	CTYRAG	1 cut(s)	206
SmoI	CTYRAG	1 cut(s)	206
SseBI	AGGCCT	1 cut(s)	579
SsiI	CCGC	5 cut(s)	85, 133, 491, 828, 1169
SspI	AATATT	1 cut(s)	347
SspMI	CTAG	3 cut(s)	1052, 1125, 1139
StuI	AGGCCT	1 cut(s)	579
StyD4I	CCNGG	1 cut(s)	213
StyI	CCWWGG	2 cut(s)	195, 813
TaaI	ACNGT	3 cut(s)	571, 1029, 1204
TaqI	TCGA	5 cut(s)	5, 150, 207, 659, 927
TaqII	GACCGA	1 cut(s)	223
TfiI	GAWTC	4 cut(s)	261, 1121, 1184, 1217
Tru1I	TTAA	6 cut(s)	425, 552, 582, 909, 939, 1040
Tru9I	TTAA	6 cut(s)	425, 552, 582, 909, 939, 1040
TscAI	CASTG	2 cut(s)	361, 606
TseFI	GTSAC	3 cut(s)	43, 597, 726
TseI	GCWGC	3 cut(s)	202, 1166, 1226
Tsp45I	GTSAC	3 cut(s)	43, 597, 726
TspDTI	ATGAA	5 cut(s)	260, 410, 516, 1050, 1283
TspGWI	ACGGA	1 cut(s)	347
TspRI	CASTG	2 cut(s)	361, 606
Tth111I	GACNNNGTC	2 cut(s)	41, 233
Van91I	CCANNNNNTGG	1 cut(s)	415
VneI	GTGCAC	1 cut(s)	357
VpaK11BI	GGWCC	3 cut(s)	235, 240, 1303
XapI	RAATTY	2 cut(s)	465, 703
XbaI	TCTAGA	1 cut(s)	1138
XhoI	CTCGAG	1 cut(s)	206
XmiI	GTMKAC	1 cut(s)	927
XmnI	GAANNNNTTC	1 cut(s)	1274
XspI	CTAG	3 cut(s)	1052, 1125, 1139

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.