Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0003782

Physical Location & Seq

Genomic Coordinates Reverse (-)

31368911 .. 31376342

Gene Structure 7432 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1249500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3840 bp

ATGAAAATGTCGTCATCAGCAGCAGCAGCAGCAGTGGGCGTCTCTGATCCAAGCCAACTTCACTTGAAAAGGGAGCTCACTCAGATTCGAAAAGCCGCCCGCGTTCTTCGTGATCCCGGCACCACTTCCTCCTGGCGCTCTCCGCTCGCCTCCTCCTCCTCTAGATCTGCGGCGGTGGCGGCAGCTGCCCAAGCAGCAATCACTACTACTAAAACAACTGCCACTTCCACTTGGAACAACAACAACAGCATTGGGAGTAATAGAAATGGCAGCGACAGGAGGGTCTTCTTGCACAACTGGAAGAACTCCAAATCCTCCTCCAGAAACGATGACTACAACGACGATGAAGATGACGATGAAGATTACAATGATGATGATGGCATTGACGTTATTGAAGATGGCATTGATGCTTCTTCTTCAGTTGCAGAGTTGAGTGTTGATGATAGCTTGAGTGATGCGCGTACCGGAAGAGGAGGAGGAGGACCTGGAGGAGATTCAAGGAGTGACACTCAGACTCACAGCCGCTCCTCATCCACCATGCTCCGGCGTAGATATGCACACCTTTTGCCTCCTCGCAAAAACACTAACAAAACAAGTAAGAAAACCGATGCCCACTCCGACCTTTTATCCAAATATCAGAAGAAAGAGTTATTTCTTGGTAGGAACAGAAAACTAGTCGTGGACGGTCACCCAAGATCATCCGCCCGCCGTGACTTGGTCGACCAATCTGATGACACCGAAGACTACTGCAACTCGGAGGATCTTAGGCCAATTTCAGGGGCTTCCCCATTATTGCTATCCAAACTTAAGCACAAGAATTGGCCTCACTCCTCCTCTAAGTTTAGGAGAGATAATAGTATTCCAAGAGAGGACTCTTCCTATTCTTATAGCACCCCTGCATTGTCAACTAGTTCTTATAACAGGTACGCCGGTGTTCGCAATCCTAGCACTGTTGGATCTTGGGATGGCACTACAACTTCCATGAATGATGGGGATGATGAGGTTGATGACCACTTGGAATTTCCCGGCCGCCAGGGATGTGGGATTCCTTGTTATTGGTCGAAAAGGACACCCAAACACAAAGGCATGTATGGAAGCTGCTGCTCCCCATCGCTTTCTGATACTTTGAGGAGGAAAGGAAGTATCATATTTTGTGGAAGTCAAAATATTTACCCTAGACAACGTCGATCATCATCAGGTTCCAATAAGCAGAGAATTGCTTCTAGAAGTGCTCAGGGCGTCCTCCCATTGCTTACCAATAGTGGTGATGGTAGGGGAGGATCATCCATAGGAACTGGGAGAAGTGATGACGAGCTTTCCACAAACTTTGGGGAACTTGATTTGGAGGCTCTGAGTCGATTAGATGGAAGGAGGTGGTCGTCAAGCTGCCGAAGTCAAGAAGGGTTGGAGATTGTAGCTGTGAATGGAGATGGAGAAGAGGAAGGCTCACCAGAAAATATTAGGAGTTTTAGCCAGAAGTACAAGCCAATGTTCTTTGGTGAGTTGATTGGGCAAAATATTGTGGTGCAATCACTCATAAATGCTATTGCAAGGGGTCGGATTGCCCCTGTTTATCTTTTCCAGGGGCCCCGTGGAACTGGAAAAACATCAGCAGCCAGGATTTTCACTGCTTCTTTGAATTGCCTATCTCCTGATGAAAATAAGCCATGTGGCTTTTGCAGGGAGTGCTCTGATTTTATGTCCGGTAAGAACAAGGATCTAATGGAAGTTGATGGCACCAATAAGAAAGGAATTGACAAAGTTAGATACCTATTGAAAACTCTATCAGCAGCACCGCCATCGGCATCCTCGAGGTACAAGGTTTTTGTGATTGATGAGTGTCACTTGTTACCTTCTAAGACGTGGCTGGCATTTCTCAAATATCTGGAAGAACCGCCACAGAGAGTTGTCTTCATATTTATAACAACTGATTTGGACAATGTTCCCCGTACAATACAATCTCGGTGTCAGAAATACCTCTTTAACAAAATTAAAGAGAGTGACATTGTGGCCAGGTTAAGGAAAATATCTGTTGAAGAAAACCTGGATGTTGAATCAGATGCACTAGAATTAATTGCTTTGAATGCGGATGGTTCACTTCGCGACGCTGAAACTATGCTGGATCAGTTGAGTTTGCTGGGGAAAAGAATTTCAACATCCCTCGTGAATGAACTTGTGGGAGTTGTTTCTGATGAAAAGCTACTGGAACTTTTGGAATTAGCAATGTCATCAGACACTGCAGAAACAGTGAAAAGGGCCAGAGAGTTGATGGACTCCGGGGTAGATCCCATGGTTTTGATGTCTCAGTTGGCCAGCCTAATCATGGACATCATTGCTGGAACCTACAATATCAATGATATCAAACATGATTCATTCTTTGGTGACCGCAATTTGGCCGAAGCAGAACTGGAGAGACTAAAACATGCTTTGAAGATTCTTTCAGAGGCTGAAAAACAGCTTAGGGTTTCAAGTGAGCGCTCAACATGGTTTACAGCGACTCTATTACAGCTTGGTTCAGTGCCTTCTCCAGACCTTACTCGCTCATGCAGCAGGAGGCACAGCAGTAAGACTACTGAGGATGATTCGTCCAGTGCTTCAAGAGAAGCTGCCACCTACAAACAGAAGTCGAATGCCCAATATATGCTTCATAAATCAATTTCCCATGCTTCTGTTCAAAAAGCACTAAATGGAAATTCCAACCATCAGGGAGATTCATTGTCGCGAAATAACGATGCCAAGCCCTTGCATGGCCATTTCACGGACAGTGGTGCCTCAACTCCCTTGCATGATGATGTTACAGCTGGAAATGCAATCCTCAGATGTGCTAACTCTGAGAAGTTGGAGGATGTCTGGGCACAGTGTATTGAGAGATGCCATTCAAAGACACTGAGGCAACTACTACACTCTCACGGAAAACTAGTGTCTATATCTGAAGCTGAAGGTGTTCTGGTTGCTTATGTCACGTTTGAAGATGGAAGTATCAAATCTAGGGTAGAAAGGTTTGTGAGCAGTATCACTAATTCAATGGAAGTGGTTCTCAGACGCAATGTAGAGGTTAGAATTGTTCACTTGCCAGGTGGAGAGGCTTCTCTAAATGGTCCAAGTCCAGTTCACTTACCAGGAACCGTGGCGGCAATTCTTAGGGAAAGGAAAAGAGTCGGATCAAATGCCACAGATGGTTACTCAAATTGCTCTTTGTTTCTTGATGAGACCCACAAGTCCACTTCTGATTCATCAGACGTGATCGCCGAAGGCAATGGCCAAGCAAGTGTTACAAGAGAGAGCAGACAGGAGATTCCAATGCAAAGGATAGAATCCATTATTCGCGACCAAAGGTTAGAGACTGCATGGCTACAGGTGGCAGAGAAAGGCACTCCTGGTTCTTTGAGTCATTTGAAACCTGAGAAGAATCAGGTCCTGCCGCAGGATGGCACTTATTATGAAGATCAGATGGAGTCATTGAATTCAATGAGGTTGTCCTCTCAGCATGGGGAAGATGGATTAAACCCTGAAGTCAAAATTTTGAAGGTTAATAGTGGAAGGGACATCCCGAAGGACCCGTTAGGTAGAAGGGTTGACCGTTATCCTATGTCCCCAAGTTTACTACATGATAGTAACTTTGTGGGAAATTCCAACAAAGATAACCAGGGTGATGAATCGGGATCAGGTAAAGGAGGAGGTTGTAGTGGATTTTTCCATTGTTATAAGACTAAACCTCGTAAAAGAGGAAAGATAAAAGGGGGGACGGCTGTTCAGGCATGCAAAGGCAGACGGTATTCATTGTTTGGGGAGTGTGGGAAGAAGTCAAGGAAAACAGAGAGCAAACATAATACAAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0000904 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005663 GO:0005694 GO:0005886 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009888 GO:0009987 GO:0010026 GO:0010090 GO:0010091 GO:0016020 GO:0016043 GO:0030154 GO:0032502 GO:0032989 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0046483 GO:0048468 GO:0048856 GO:0048869 GO:0050896 GO:0051716 GO:0071704 GO:0071840 GO:0071944 GO:0090304 GO:0090558 GO:0090626 GO:1901360 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DNA_pol3_delta2 DNA_pol3_gamma3

Protein Analysis

1280

Amino Acids

140.5

Weight (kDa)

8.48

Isoelectric Point (pI)

49.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_pol3_delta2	PF13177	505 - 664	5.4e-35	DNA polymerase III, delta subunit
AAA	PF00004	526 - 661	9.4e-08	ATPase family associated with various cellular activities (AAA)
DNAX_ATPase_lid	PF22608	669 - 709	4.9e-09	DNA polymerase III clamp loader subunit, ATPase lid domain
DNA_pol3_gamma3	PF12169	718 - 830	1.1e-07	DNA polymerase III subunits gamma and tau domain III
DnaA_N-like_STI	PF23007	940 - 1021	2.7e-30	STICHEL, DnaA_N-like alpha-beta domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0003782)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G14460 AT2G02480 AT2G02480
fragaria_vesca	FvH4_5g27450 FvH4_5g27450
malus_domestica	MD08G1249500.v1.1
prunus_persica	Prupe.1G586700_v2.0.a1 Prupe.1G586700_v2.0.a1 Prupe.1G586700_v2.0.a1 Prupe.1G586700_v2.0.a1 Prupe.1G586700_v2.0.a1
pyrus_communis	pycom08g21590
rosa_chinensis	RchiOBHm_Chr2g0146641 RchiOBHm_Chr7g0218871
rosa_laevigata	RLG00000001635 RLG00000002346 RLG00000002355
rosa_multiflora	Rmu_sc0003823.1_g000001 Rmu_sc0006998.1_g000001
rosa_roxburghii	Rroxscaffold_3G00240440
rosa_rugosa	Rorug07G0179300
rosa_samantha	Rh3AG021300 Rh3CG020600 Rh5BG537900 Rh5CG561800 Rh7AG322600 Rh7BG313700 Rh7CG339400 Rh7DG319800 Rh7DG389400
rosa_wichuraiana	Rw5G027500 Rw7G026930 Rw7G027310 Rw7G028430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	918, 1922, 3705
AasI	GACNNNNNNGTC	2 cut(s)	281, 3217
AbsI	CCTCGAGG	1 cut(s)	1810
AccB1I	GGYRCC	3 cut(s)	119, 1736, 2768
AccBSI	CCGCTC	2 cut(s)	145, 525
AccI	GTMKAC	1 cut(s)	720
AccII	CGCG	5 cut(s)	102, 460, 2103, 2722, 3327
AcoI	YGGCCR	6 cut(s)	1027, 2010, 2310, 2394, 2749, 3259
AcsI	RAATTY	6 cut(s)	1019, 2148, 2692, 3463, 3519, 3628
AcuI	CTGAAG	4 cut(s)	402, 2952, 2958, 3531
AcyI	GRCGYC	2 cut(s)	39, 1239
AfaI	GTAC	5 cut(s)	463, 926, 1481, 1817, 1951
AfeI	AGCGCT	1 cut(s)	2477
AflII	CTTAAG	1 cut(s)	806
AhlI	ACTAGT	3 cut(s)	673, 908, 2917
AjiI	CACGTC	2 cut(s)	1863, 3241
AjuI	GAANNNNNNNTTGG	2 cut(s)	2628, 2660
AloI	GAACNNNNNNTCC	2 cut(s)	296, 328
Alw21I	GWGCWC	3 cut(s)	78, 1234, 1691
Alw26I	GTCTC	5 cut(s)	46, 2307, 2407, 3203, 3335
AlwNI	CAGNNNCTG	3 cut(s)	2237, 2447, 3418
Ama87I	CYCGRG	1 cut(s)	1810
Aor51HI	AGCGCT	1 cut(s)	2477
ApaI	GGGCCC	1 cut(s)	1590
ApoI	RAATTY	6 cut(s)	1019, 2148, 2692, 3463, 3519, 3628
AseI	ATTAAT	1 cut(s)	2072
Asp700I	GAANNNNTTC	3 cut(s)	1219, 2943, 3033
AspLEI	GCGC	3 cut(s)	138, 460, 2478
AspS9I	GGNCC	7 cut(s)	482, 1586, 1587, 2256, 3098, 3415, 3556
AsuC2I	CCSGG	3 cut(s)	117, 1026, 2278
AsuHPI	GGTGA	6 cut(s)	680, 1277, 1440, 1511, 2393, 3662
AsuII	TTCGAA	1 cut(s)	88
AvaI	CYCGRG	1 cut(s)	1810
AvaII	GGWCC	4 cut(s)	482, 3098, 3415, 3556
BaeGI	GKGCMC	2 cut(s)	1590, 2857
BaeI	ACNNNNGTAYC	2 cut(s)	916, 949
BalI	TGGCCA	4 cut(s)	2012, 2312, 2751, 3261
BanI	GGYRCC	3 cut(s)	119, 1736, 2768
BanII	GRGCYC	2 cut(s)	78, 1590
BauI	CACGAG	1 cut(s)	2162
BbsI	GAAGAC	3 cut(s)	277, 747, 1903
Bbv12I	GWGCWC	3 cut(s)	78, 1234, 1691
BceAI	ACGGC	2 cut(s)	693, 3762
BcgI	CGANNNNNNTGC	2 cut(s)	1782, 1816
BcnI	CCSGG	3 cut(s)	117, 1026, 2278
BcoDI	GTCTC	5 cut(s)	46, 2307, 2407, 3203, 3335
BcuI	ACTAGT	3 cut(s)	673, 908, 2917
BfaI	CTAG	9 cut(s)	162, 674, 909, 945, 1176, 1224, 2066, 2918, 2988
BfmI	CTRYAG	2 cut(s)	2238, 3353
BfoI	RGCGCY	2 cut(s)	139, 2479
BfrI	CTTAAG	1 cut(s)	806
BglII	AGATCT	1 cut(s)	164
Bme18I	GGWCC	4 cut(s)	482, 3098, 3415, 3556
BmeT110I	CYCGRG	1 cut(s)	1810
BmgBI	CACGTC	2 cut(s)	1863, 3241
BmgT120I	GGNCC	7 cut(s)	482, 1586, 1587, 2256, 3098, 3415, 3556
BmrI	ACTGGG	1 cut(s)	1305
BmsI	GCATC	7 cut(s)	397, 445, 598, 1814, 2050, 2722, 2861
BmuI	ACTGGG	1 cut(s)	1305
BpiI	GAAGAC	3 cut(s)	277, 747, 1903
BplI	GAGNNNNNCTC	4 cut(s)	493, 525, 1430, 1462
BpmI	CTGGAG	4 cut(s)	304, 507, 2429, 2511
Bpu10I	CCTNAGC	2 cut(s)	1233, 2459
Bpu14I	TTCGAA	1 cut(s)	88
BpuEI	CTTGAG	1 cut(s)	469
BpuMI	CCSGG	3 cut(s)	117, 1026, 2278
BsaBI	GATNNNNATC	1 cut(s)	1192
BsaHI	GRCGYC	2 cut(s)	39, 1239
BsaI	GGTCTC	1 cut(s)	3203
BsaJI	CCNNGG	7 cut(s)	1033, 1582, 1591, 2277, 2289, 3126, 3646
BsaWI	WCCGGW	2 cut(s)	464, 1703
BsaXI	ACNNNNNCTCC	2 cut(s)	509, 539
Bse118I	RCCGGY	1 cut(s)	929
Bse1I	ACTGG	7 cut(s)	302, 1300, 1603, 2208, 2412, 2589, 3107
Bse3DI	GCAATG	5 cut(s)	1247, 2229, 2331, 3052, 3262
Bse8I	GATNNNNATC	1 cut(s)	1192
BseDI	CCNNGG	7 cut(s)	1033, 1582, 1591, 2277, 2289, 3126, 3646
BseJI	GATNNNNATC	1 cut(s)	1192
BseMI	GCAATG	5 cut(s)	1247, 2229, 2331, 3052, 3262
BseNI	ACTGG	7 cut(s)	302, 1300, 1603, 2208, 2412, 2589, 3107
BseSI	GKGCMC	2 cut(s)	1590, 2857
BseX3I	CGGCCG	1 cut(s)	1027
BseYI	CCCAGC	1 cut(s)	2137
Bsh1236I	CGCG	5 cut(s)	102, 460, 2103, 2722, 3327
Bsh1285I	CGRYCG	1 cut(s)	1030
BshNI	GGYRCC	3 cut(s)	119, 1736, 2768
BsiEI	CGRYCG	1 cut(s)	1030
BsiHKAI	GWGCWC	3 cut(s)	78, 1234, 1691
BsiHKCI	CYCGRG	1 cut(s)	1810
BsiSI	CCGG	7 cut(s)	117, 465, 544, 930, 1026, 1704, 2277
BslFI	GGGAC	3 cut(s)	3557, 3577, 3757
BsmAI	GTCTC	5 cut(s)	46, 2307, 2407, 3203, 3335
BsmBI	CGTCTC	1 cut(s)	46
BsmFI	GGGAC	3 cut(s)	3557, 3577, 3757
BsmI	GAATGC	2 cut(s)	2089, 2635
Bso31I	GGTCTC	1 cut(s)	3203
BsoBI	CYCGRG	1 cut(s)	1810
Bsp119I	TTCGAA	1 cut(s)	88
Bsp120I	GGGCCC	1 cut(s)	1586
Bsp1286I	GDGCHC	5 cut(s)	78, 1234, 1590, 1691, 2857
Bsp19I	CCATGG	1 cut(s)	2289
Bsp68I	TCGCGA	3 cut(s)	2103, 2722, 3327
BspFNI	CGCG	5 cut(s)	102, 460, 2103, 2722, 3327
BspMAI	CTGCAG	1 cut(s)	2242
BspT104I	TTCGAA	1 cut(s)	88
BspT107I	GGYRCC	3 cut(s)	119, 1736, 2768
BspTI	CTTAAG	1 cut(s)	806
BspTNI	GGTCTC	1 cut(s)	3203
BsrBI	CCGCTC	2 cut(s)	145, 525
BsrDI	GCAATG	5 cut(s)	1247, 2229, 2331, 3052, 3262
BsrFI	RCCGGY	1 cut(s)	929
BsrI	ACTGG	7 cut(s)	302, 1300, 1603, 2208, 2412, 2589, 3107
BssAI	RCCGGY	1 cut(s)	929
BssECI	CCNNGG	7 cut(s)	1033, 1582, 1591, 2277, 2289, 3126, 3646
BssNI	GRCGYC	2 cut(s)	39, 1239
BssSI	CACGAG	1 cut(s)	2162
BssT1I	CCWWGG	1 cut(s)	2289
Bst2BI	CACGAG	1 cut(s)	2162
Bst4CI	ACNGT	8 cut(s)	686, 952, 2248, 2765, 2859, 3127, 3581, 3774
Bst6I	CTCTTC	3 cut(s)	463, 880, 1431
BstACI	GRCGYC	2 cut(s)	39, 1239
BstAFI	CTTAAG	1 cut(s)	806
BstAPI	GCANNNNNTGC	1 cut(s)	1686
BstBI	TTCGAA	1 cut(s)	88
BstC8I	GCNNGC	6 cut(s)	100, 147, 706, 1869, 2314, 3760
BstDSI	CCRYGG	3 cut(s)	1591, 2289, 3126
BstEII	GGTNACC	2 cut(s)	686, 2381
BstENI	CCTNNNNNAGG	1 cut(s)	3422
BstFNI	CGCG	5 cut(s)	102, 460, 2103, 2722, 3327
BstH2I	RGCGCY	2 cut(s)	139, 2479
BstHHI	GCGC	3 cut(s)	138, 460, 2478
BstMAI	GTCTC	5 cut(s)	46, 2307, 2407, 3203, 3335
BstMCI	CGRYCG	1 cut(s)	1030
BstNSI	RCATGY	3 cut(s)	1090, 2426, 3762
BstPI	GGTNACC	2 cut(s)	686, 2381
BstSFI	CTRYAG	2 cut(s)	2238, 3353
BstSLI	GKGCMC	2 cut(s)	1590, 2857
BstUI	CGCG	5 cut(s)	102, 460, 2103, 2722, 3327
BstV2I	GAAGAC	3 cut(s)	277, 747, 1903
BstX2I	RGATCY	5 cut(s)	164, 760, 956, 1717, 2284
BstXI	CCANNNNNNTGG	1 cut(s)	1040
BstYI	RGATCY	5 cut(s)	164, 760, 956, 1717, 2284
BstZI	CGGCCG	1 cut(s)	1027
BtgI	CCRYGG	3 cut(s)	1591, 2289, 3126
BtgZI	GCGATG	1 cut(s)	1095
BtrI	CACGTC	2 cut(s)	1863, 3241
BtsI	GCAGTG	3 cut(s)	39, 1626, 2235
BtuMI	TCGCGA	3 cut(s)	2103, 2722, 3327
Cac8I	GCNNGC	6 cut(s)	100, 147, 706, 1869, 2314, 3760
CaiI	CAGNNNCTG	3 cut(s)	2237, 2447, 3418
CfoI	GCGC	3 cut(s)	138, 460, 2478
Cfr10I	RCCGGY	1 cut(s)	929
Cfr13I	GGNCC	7 cut(s)	482, 1586, 1587, 2256, 3098, 3415, 3556
CseI	GACGC	4 cut(s)	28, 1228, 2114, 3051
Csp6I	GTAC	5 cut(s)	462, 925, 1480, 1816, 1950
CspCI	CAANNNNNGTGG	4 cut(s)	1309, 1344, 3012, 3047
CviQI	GTAC	5 cut(s)	462, 925, 1480, 1816, 1950
DrdI	GACNNNNNNGTC	2 cut(s)	281, 3217
DseDI	GACNNNNNNGTC	2 cut(s)	281, 3217
EaeI	YGGCCR	6 cut(s)	1027, 2010, 2310, 2394, 2749, 3259
EagI	CGGCCG	1 cut(s)	1027
Eam1104I	CTCTTC	3 cut(s)	463, 880, 1431
EarI	CTCTTC	3 cut(s)	463, 880, 1431
EciI	GGCGGA	1 cut(s)	691
Ecl136II	GAGCTC	1 cut(s)	76
EclXI	CGGCCG	1 cut(s)	1027
Eco130I	CCWWGG	1 cut(s)	2289
Eco24I	GRGCYC	2 cut(s)	78, 1590
Eco31I	GGTCTC	1 cut(s)	3203
Eco32I	GATATC	1 cut(s)	2359
Eco47I	GGWCC	4 cut(s)	482, 3098, 3415, 3556
Eco47III	AGCGCT	1 cut(s)	2477
Eco52I	CGGCCG	1 cut(s)	1027
Eco53kI	GAGCTC	1 cut(s)	76
Eco57I	CTGAAG	4 cut(s)	402, 2952, 2958, 3531
Eco88I	CYCGRG	1 cut(s)	1810
Eco91I	GGTNACC	2 cut(s)	686, 2381
EcoICRI	GAGCTC	1 cut(s)	76
EcoNI	CCTNNNNNAGG	1 cut(s)	3422
EcoO109I	RGGNCCY	5 cut(s)	482, 1586, 1587, 3415, 3556
EcoO65I	GGTNACC	2 cut(s)	686, 2381
EcoRI	GAATTC	1 cut(s)	3463
EcoRV	GATATC	1 cut(s)	2359
EcoT14I	CCWWGG	1 cut(s)	2289
EcoT38I	GRGCYC	2 cut(s)	78, 1590
ErhI	CCWWGG	1 cut(s)	2289
Esp3I	CGTCTC	1 cut(s)	46
FaqI	GGGAC	3 cut(s)	3557, 3577, 3757
FauI	CCCGC	2 cut(s)	107, 713
FblI	GTMKAC	1 cut(s)	720
FriOI	GRGCYC	2 cut(s)	78, 1590
FspBI	CTAG	9 cut(s)	162, 674, 909, 945, 1176, 1224, 2066, 2918, 2988
GlaI	GCGC	3 cut(s)	137, 459, 2477
GsaI	CCCAGC	1 cut(s)	2141
GsuI	CTGGAG	4 cut(s)	304, 507, 2429, 2511
HaeII	RGCGCY	2 cut(s)	139, 2479
HapII	CCGG	7 cut(s)	117, 465, 544, 930, 1026, 1704, 2277
HgaI	GACGC	4 cut(s)	28, 1228, 2114, 3051
HhaI	GCGC	3 cut(s)	138, 460, 2478
Hin1I	GRCGYC	2 cut(s)	39, 1239
Hin6I	GCGC	3 cut(s)	136, 458, 2476
HinP1I	GCGC	3 cut(s)	136, 458, 2476
HincII	GTYRAC	3 cut(s)	721, 906, 3577
HindII	GTYRAC	3 cut(s)	721, 906, 3577
HpaII	CCGG	7 cut(s)	117, 465, 544, 930, 1026, 1704, 2277
HphI	GGTGA	6 cut(s)	680, 1277, 1440, 1511, 2393, 3662
Hpy99I	CGWCG	3 cut(s)	344, 1188, 2108
HpyCH4III	ACNGT	8 cut(s)	686, 952, 2248, 2765, 2859, 3127, 3581, 3774
HpyCH4IV	ACGT	5 cut(s)	387, 1183, 1862, 2963, 3240
HpySE526I	ACGT	5 cut(s)	387, 1183, 1862, 2963, 3240
Hsp92I	GRCGYC	2 cut(s)	39, 1239
HspAI	GCGC	3 cut(s)	136, 458, 2476
LmnI	GCTCC	4 cut(s)	73, 530, 546, 1109
LweI	GCATC	7 cut(s)	397, 445, 598, 1814, 2050, 2722, 2861
MaeI	CTAG	9 cut(s)	162, 674, 909, 945, 1176, 1224, 2066, 2918, 2988
MaeII	ACGT	5 cut(s)	387, 1183, 1862, 2963, 3240
MbiI	CCGCTC	2 cut(s)	145, 525
MflI	RGATCY	5 cut(s)	164, 760, 956, 1717, 2284
MhlI	GDGCHC	5 cut(s)	78, 1234, 1590, 1691, 2857
MlsI	TGGCCA	4 cut(s)	2012, 2312, 2751, 3261
MluNI	TGGCCA	4 cut(s)	2012, 2312, 2751, 3261
MlyI	GAGTC	8 cut(s)	508, 866, 1363, 2267, 2491, 3165, 3397, 3464
MmeI	TCCRAC	8 cut(s)	642, 934, 1386, 1538, 2721, 2820, 3139, 3657
Mox20I	TGGCCA	4 cut(s)	2012, 2312, 2751, 3261
MroXI	GAANNNNTTC	3 cut(s)	1219, 2943, 3033
MscI	TGGCCA	4 cut(s)	2012, 2312, 2751, 3261
MseI	TTAA	7 cut(s)	807, 1983, 1992, 2018, 2072, 3503, 3531
MslI	CAYNNNNRTG	1 cut(s)	2790
Msp20I	TGGCCA	4 cut(s)	2012, 2312, 2751, 3261
MspA1I	CMGCKG	2 cut(s)	185, 2801
MspCI	CTTAAG	1 cut(s)	806
MspI	CCGG	7 cut(s)	117, 465, 544, 930, 1026, 1704, 2277
Mva1269I	GAATGC	2 cut(s)	2089, 2635
MvnI	CGCG	5 cut(s)	102, 460, 2103, 2722, 3327
NciI	CCSGG	3 cut(s)	117, 1026, 2278
NcoI	CCATGG	1 cut(s)	2289
NmuCI	GTSAC	7 cut(s)	503, 686, 710, 1841, 2000, 2381, 2959
NruI	TCGCGA	3 cut(s)	2103, 2722, 3327
NspI	RCATGY	3 cut(s)	1090, 2426, 3762
NspV	TTCGAA	1 cut(s)	88
PaeI	GCATGC	1 cut(s)	3762
PaeR7I	CTCGAG	1 cut(s)	1810
PcsI	WCGNNNNNNNCGW	2 cut(s)	1808, 3246
PctI	GAATGC	2 cut(s)	2089, 2635
PdmI	GAANNNNTTC	3 cut(s)	1219, 2943, 3033
PflFI	GACNNNGTC	2 cut(s)	716, 1182
PleI	GAGTC	8 cut(s)	508, 866, 1362, 2267, 2491, 3164, 3396, 3463
PpsI	GAGTC	8 cut(s)	508, 866, 1362, 2267, 2491, 3164, 3396, 3463
PpuMI	RGGWCCY	3 cut(s)	482, 3415, 3556
PshBI	ATTAAT	1 cut(s)	2072
PsiI	TTATAA	3 cut(s)	918, 1922, 3705
Psp124BI	GAGCTC	1 cut(s)	78
Psp5II	RGGWCCY	3 cut(s)	482, 3415, 3556
PspEI	GGTNACC	2 cut(s)	686, 2381
PspFI	CCCAGC	1 cut(s)	2137
PspOMI	GGGCCC	1 cut(s)	1586
PspPI	GGNCC	7 cut(s)	482, 1586, 1587, 2256, 3098, 3415, 3556
PspPPI	RGGWCCY	3 cut(s)	482, 3415, 3556
PspXI	VCTCGAGB	1 cut(s)	1810
PstI	CTGCAG	1 cut(s)	2242
PstNI	CAGNNNCTG	3 cut(s)	2237, 2447, 3418
PsuI	RGATCY	5 cut(s)	164, 760, 956, 1717, 2284
PsyI	GACNNNGTC	2 cut(s)	716, 1182
PvuII	CAGCTG	2 cut(s)	185, 2801
RruI	TCGCGA	3 cut(s)	2103, 2722, 3327
RsaI	GTAC	5 cut(s)	463, 926, 1481, 1817, 1951
RsaNI	GTAC	5 cut(s)	462, 925, 1480, 1816, 1950
RseI	CAYNNNNRTG	1 cut(s)	2790
SacI	GAGCTC	1 cut(s)	78
SalI	GTCGAC	1 cut(s)	719
SaqAI	TTAA	7 cut(s)	807, 1983, 1992, 2018, 2072, 3503, 3531
Sau96I	GGNCC	7 cut(s)	482, 1586, 1587, 2256, 3098, 3415, 3556
SchI	GAGTC	8 cut(s)	508, 866, 1363, 2267, 2491, 3165, 3397, 3464
SduI	GDGCHC	5 cut(s)	78, 1234, 1590, 1691, 2857
SfaNI	GCATC	7 cut(s)	397, 445, 598, 1814, 2050, 2722, 2861
SfcI	CTRYAG	2 cut(s)	2238, 3353
Sfr274I	CTCGAG	1 cut(s)	1810
SfuI	TTCGAA	1 cut(s)	88
SgrAI	CRCCGGYG	1 cut(s)	929
SinI	GGWCC	4 cut(s)	482, 3098, 3415, 3556
SlaI	CTCGAG	1 cut(s)	1810
SmiMI	CAYNNNNRTG	1 cut(s)	2790
SmlI	CTYRAG	3 cut(s)	448, 806, 1810
SmoI	CTYRAG	3 cut(s)	448, 806, 1810
SpeI	ACTAGT	3 cut(s)	673, 908, 2917
SphI	GCATGC	1 cut(s)	3762
SspI	AATATT	3 cut(s)	1168, 1459, 1519
SspMI	CTAG	9 cut(s)	162, 674, 909, 945, 1176, 1224, 2066, 2918, 2988
SstI	GAGCTC	1 cut(s)	78
StyI	CCWWGG	1 cut(s)	2289
TaaI	ACNGT	8 cut(s)	686, 952, 2248, 2765, 2859, 3127, 3581, 3774
TaiI	ACGT	5 cut(s)	390, 1186, 1865, 2966, 3243
TaqI	TCGA	7 cut(s)	88, 720, 1061, 1186, 1357, 1811, 2627
TatI	WGTACW	1 cut(s)	1479
TauI	GCSGC	7 cut(s)	98, 173, 182, 525, 1032, 3134, 3424
Tru1I	TTAA	7 cut(s)	807, 1983, 1992, 2018, 2072, 3503, 3531
Tru9I	TTAA	7 cut(s)	807, 1983, 1992, 2018, 2072, 3503, 3531
TseFI	GTSAC	7 cut(s)	503, 686, 710, 1841, 2000, 2381, 2959
Tsp45I	GTSAC	7 cut(s)	503, 686, 710, 1841, 2000, 2381, 2959
TspGWI	ACGGA	2 cut(s)	2774, 2925
Tth111I	GACNNNGTC	2 cut(s)	716, 1182
Vha464I	CTTAAG	1 cut(s)	806
VpaK11BI	GGWCC	4 cut(s)	482, 3098, 3415, 3556
VspI	ATTAAT	1 cut(s)	2072
XagI	CCTNNNNNAGG	1 cut(s)	3422
XapI	RAATTY	6 cut(s)	1019, 2148, 2692, 3463, 3519, 3628
XbaI	TCTAGA	2 cut(s)	161, 1223
XceI	RCATGY	3 cut(s)	1090, 2426, 3762
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1589, 2266, 2320
XhoI	CTCGAG	1 cut(s)	1810
XmiI	GTMKAC	1 cut(s)	720
XmnI	GAANNNNTTC	3 cut(s)	1219, 2943, 3033
XspI	CTAG	9 cut(s)	162, 674, 909, 945, 1176, 1224, 2066, 2918, 2988

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.