Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0003782

Physical Location & Seq

Genomic Coordinates Reverse (-)

32110095 .. 32117745

Gene Structure 7651 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00240440.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3513 bp

ATGGGGGTGTCTGATCCAAGCCAACTCCACTTGAAGAAAGAGCTCACCCAGATTCGAAAGGCCGCCCGCGTCCTCCGCGATCCGGGGACCACTTCTTCTTGGCGCTCGCCTTTGGCTTCCTCTAGATCTGCGGCGGCGGCGCCGGTTCCTTCTACTTGTTCCACTTCCCACAATACCAACAATGGCGGCGGCGACAACAAGAAGGTGTTCTTGTACAACTGGAAGGGCTCTTCCAAATCGTCGAGAAATGATGACTACGAAGAGGATGAGGAGGAGGATGATTATGGTGTTGAAGATGAGGATGAAGCTTCTTCTGTTGCGGCATTGAGTGTGGATGACAGCTTGAGCCACGGAGATTCCAGGAGTGACACTGGTGGTGGTCAGAGCCGGTCGTTGATGATGCTCCGGCGCAGATATCCGAATCTTCTACCGCCGCTCAAAGATGGTAATGGTAAGAAAGCAAGATCTGAGGCACATGAGGGGGCTTCCCCATTGCTCTTGAAGCTTAGGCACAAGCATTGGCCTCCGGCCTCGACTAATGTTAGGAGAAATGATAGTAGAGCAGAGGACTCTTCTTATTGCTATAGCACTCCTGCATTGTCGACTAGTTCTTATAATAGGTATGGTGTTCGGAATCCAAGTACTGTTGGATCTTGGGATGGAACTGCGACTTCAATGAATGATGGGGATGATGAGGTTGAGGACCATTTGGATTTTTCGGGTAGCAAAGGATGTGGGATTCCTTGTTATTGGTCCAAAAAGACTCCAAAGCACAGAGGCATGTATGGAAGCTGTTGCTCCCCTTCGCTTTCTGATACTATAAGGAGAAAAGGAAGTGTCCTTTTGTGTGGAAGTCAAAATGTTTATCCTAGACGACGGGGGTCGTCGTCAGGTTCCAATAAGCAGAGAATTGCATCTAGAAGTGCGCAAGGTGTTCTGCCATTGCTAAACAATAGTGGTGATGGTAGAGGAGGGTCATCCATAGGAACTGGGAGGAGTGATGACGAACTCTCCACAAACTTTGGGGAACTTGATTTGGAAGCTTTGAGTAGATTGGATGGACGGAGATGGTCAAGCTGCCGAAGTCAAGAAGGCTTGGAGATTGTAGCTCTGAGTGGAGTGGGGGAAGACGAAAGCACTCCAGATAGTGTTAGAAGTTTTAGCCAGAAGTACAAGCCGATGTTCTTTCGTGAATTGATTGGCCAGAATATTGTGGTGCAATCACTTATAAATGCTATTGATAGGTGTAGGATTGCTCCCGTTTATCTTTTCCAGGGACCCCGTGGAACTGGAAAAACATCAACAGCTAGAATTTTTGCTGCTTCCTTGAATTGCCTAGCTCCTGATGAAACTAAACCCTGTGGCTACTGCAGGGAGTGCACTGATTTTATGTCTGGTAAGAACAAGGATCTATTGGAAGTTGATGGTACCAATAAGAAAGGAATTGATAAAGTCAGGTACCTATTGAAAACTCTATCTGGGGCTCCCTCATCAGCATCGTCACGGTATAAGGTTTTTGTAATTGATGAGTGTCACTTATTGCCTTCCAAGACATGGTTGGCATTTCTTAAATATTTGGAAGAACCACCGCAGCGAGTTGTCTTCATATTCATTACAACTGATTTGGATAATGTTCCTCGTACCATACAATCTCGGTGTCAGAAATATCTCTTCAACAAAATTAAGGATAGTGAGATTGTGGCCAGGTTGAGGAAAATTGCTGTTGAAGAGAACCTAGCTGTTGAACCAGATGCATTAGATTTGATTGCTTTGAATGCAGATGGTTCACTTCGAGATGCAGAAACTATGTTGGACCAGTTGAGTTTGCTGGGGAAAAGAATTTCAACATCTCTTGTGAATGAACTTGTGGGAGTTGTTTCCGATGACAAGTTACTAGATCTTTTGGAGTTAGCAATGTCATCGGACACTGCAGAAACAGTGAAAAGGGCCAGAGATTTGATGGACTGCGGGGTTGATCCCATGGTTTTGATGTCTCAGTTGGCCAGCTTAATTATGGACATCATTGCGGGAACCAACAGTACTAACGATGTCAAACATGACTCCTTATATGGTGCTCGGAACTTGACCGAAGCAGAATTGGAGAGATTAAAACATGCTCTGAAGATTCTTTCGGAGGCTGAAAAACAGCTTAGGGTTTCAAGTGAGCGCTCAACATGGTTTACAGCGACTCTGTTGCAGCTTGGTTCAGTGCCTTCTCCAGACCTTAGTCACTCATGCAGCAGGAGGCATAGCTGCAAGACAACTGAGGATGATTCTTCAAGTGCTTCAACTTCAAGAGAAGCAGCCACTTACAAACAGAAGTTAGACGGTCACCATATGCTACAAAAATCAACTCAACATGCTTCTGTACAAAGAGCACCAAATGACAATTCCAACCATCAGGGGGATTTGTTGTCAAGAAATAATGGTTTCGGTGTGAATACCAAGCCCTCACATGATCAGTTTGTGGATAGTGGAGCCTCAATTCCCTTGTGTGGAAATGTTATCGCTGGAAATATGAGCCTTAGATGTGTAAACTCTGAAAAGTTAAATGATGTATGGGCACAGTGTATTGAGAAATGTCATTCAAAGACATTGAGGCAGCTTCTACATTCTCATGGAAAGCTAGTGTCTATATCTGAAGCTGAAGATGGAAGTATCAAATCTAGAGCAGAGAGGTTTGTGAGCAGTATCACAAATTCAATGGAAATAGTTCTCAGACGCAATGTAGAGGTTAGAATTGTCCACTTGCCAGGTGGGGAGGCTTCTCTAAATGGTCCAAGTCCAGTTCACTTGCTCGAAGGTCTAAAGCAGGCAGGAACTATAGCAGAGCTTGTCAGGGAAAGGAAAAGAGTCGGTTCAAATGCCACAGATGGTTATTCCAATTGTTCTCTGTTTCTGGATGGGACCCACAAGTCAACCTCTGATTCATCAGACTTAGTTGCAGATGGCAATGCTCAAACAAGTGATACAAGAGAGAGCGGACAGGAGATCCCAATGCAGAGAATAGAATCCATTATTCGCGACCAAAGGTTAGAGACTGCGTGGCTACAGGTTGTAGAGAAAGGCACCCCTGGTTCTTTGAGTCGTTTGAAACCTGAAAAGAATCAGGTCCTGCCTCAGGATGGCATTTATTATGAAGATCAGATGGAATCGATGAATTCAATGGGATTATCCTCTCAGCAATGGGATGGTGGATTAAACCATGAAGTAAAAATCTTGAGTGCTAACAGTGGGAAGGTCATTCAGCAGGACCAAATAGGTAAAAGGGTTGAACATTATCCTATGTCCCCGAGTTTACTACATGAAAAGGGCAAGTCCGACAAAGATCACCTGGGCGATGAATCAGGGTCAGGTAGAGGAGGTTGCAGTGGATTTTTTCGTTGTTATAACACTAGACGTCGTAAAAGAGGAAAGGTAAAAGGGACCCCTGTTCAACCACGCAAAGGTAGACGGTTTTCATTGTTTGGTGTGTGTGGCAAGTCAAGGAAGAAAGCAGAGAAGACAAGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0000904 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005663 GO:0005694 GO:0005886 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009888 GO:0009987 GO:0010026 GO:0010090 GO:0010091 GO:0016020 GO:0016043 GO:0030154 GO:0032502 GO:0032989 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0046483 GO:0048468 GO:0048856 GO:0048869 GO:0050896 GO:0051716 GO:0071704 GO:0071840 GO:0071944 GO:0090304 GO:0090558 GO:0090626 GO:1901360 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DNA_pol3_delta2 DNA_pol3_gamma3

Protein Analysis

1170

Amino Acids

128.39

Weight (kDa)

8.49

Isoelectric Point (pI)

47.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_pol3_delta2	PF13177	402 - 561	3.6e-34	DNA polymerase III, delta subunit
AAA	PF00004	423 - 558	8.4e-09	ATPase family associated with various cellular activities (AAA)
DNAX_ATPase_lid	PF22608	565 - 606	9e-10	DNA polymerase III clamp loader subunit, ATPase lid domain
DNA_pol3_gamma3	PF12169	615 - 736	3.3e-08	DNA polymerase III subunits gamma and tau domain III
DnaA_N-like_STI	PF23007	843 - 914	4.6e-22	STICHEL, DnaA_N-like alpha-beta domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0003782)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G14460 AT2G02480 AT2G02480
fragaria_vesca	FvH4_5g27450 FvH4_5g27450
malus_domestica	MD08G1249500.v1.1
prunus_persica	Prupe.1G586700_v2.0.a1 Prupe.1G586700_v2.0.a1 Prupe.1G586700_v2.0.a1 Prupe.1G586700_v2.0.a1 Prupe.1G586700_v2.0.a1
pyrus_communis	pycom08g21590
rosa_chinensis	RchiOBHm_Chr2g0146641 RchiOBHm_Chr7g0218871
rosa_laevigata	RLG00000001635 RLG00000002346 RLG00000002355
rosa_multiflora	Rmu_sc0003823.1_g000001 Rmu_sc0006998.1_g000001
rosa_roxburghii	Rroxscaffold_3G00240440
rosa_rugosa	Rorug07G0179300
rosa_samantha	Rh3AG021300 Rh3CG020600 Rh5BG537900 Rh5CG561800 Rh7AG322600 Rh7BG313700 Rh7CG339400 Rh7DG319800 Rh7DG389400
rosa_wichuraiana	Rw5G027500 Rw7G026930 Rw7G027310 Rw7G028430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	615, 1229, 3390
AasI	GACNNNNNNGTC	1 cut(s)	2914
AatII	GACGTC	1 cut(s)	3403
Acc16I	TGCGCA	1 cut(s)	927
Acc65I	GGTACC	2 cut(s)	1427, 1458
AccB1I	GGYRCC	4 cut(s)	139, 1427, 1458, 3068
AccB7I	CCANNNNNTGG	1 cut(s)	1554
AccBSI	CCGCTC	2 cut(s)	436, 2982
AccI	GTMKAC	2 cut(s)	602, 3451
AccII	CGCG	3 cut(s)	69, 78, 3024
AclWI	GGATC	6 cut(s)	8, 74, 658, 1416, 1970, 2986
AcoI	YGGCCR	3 cut(s)	1201, 1701, 2001
AcsI	RAATTY	4 cut(s)	1311, 1839, 2698, 3160
AcuI	CTGAAG	3 cut(s)	2141, 2661, 2667
AcyI	GRCGYC	2 cut(s)	140, 3400
AfaI	GTAC	8 cut(s)	215, 643, 1172, 1429, 1460, 1642, 2041, 2370
AfeI	AGCGCT	1 cut(s)	2168
AfiI	CCNNNNNNNGG	8 cut(s)	82, 1554, 1710, 2404, 2495, 3121, 3125, 3446
AhlI	ACTAGT	1 cut(s)	605
AjnI	CCWGG	6 cut(s)	359, 1272, 1703, 2752, 3073, 3333
AjuI	GAANNNNNNNTTGG	2 cut(s)	2374, 2406
Alw21I	GWGCWC	4 cut(s)	45, 1382, 2077, 2380
Alw26I	GTCTC	2 cut(s)	1998, 3032
Alw44I	GTGCAC	1 cut(s)	1378
AlwI	GGATC	6 cut(s)	8, 74, 658, 1416, 1970, 2986
AlwNI	CAGNNNCTG	1 cut(s)	3115
Ama87I	CYCGRG	1 cut(s)	3292
Aor51HI	AGCGCT	1 cut(s)	2168
AoxI	GGCC	7 cut(s)	60, 521, 528, 1201, 1701, 1947, 2001
ApaLI	GTGCAC	1 cut(s)	1378
ApeKI	GCWGC	8 cut(s)	1077, 1319, 1591, 2197, 2238, 2253, 2303, 2602
ApoI	RAATTY	4 cut(s)	1311, 1839, 2698, 3160
Asp700I	GAANNNNTTC	2 cut(s)	206, 2712
Asp718I	GGTACC	2 cut(s)	1427, 1458
AspLEI	GCGC	5 cut(s)	105, 142, 411, 928, 2169
AsuC2I	CCSGG	1 cut(s)	84
AsuHPI	GGTGA	4 cut(s)	37, 971, 2324, 3323
AsuII	TTCGAA	1 cut(s)	55
AvaI	CYCGRG	1 cut(s)	3292
AxyI	CCTNAGG	1 cut(s)	3120
BaeGI	GKGCMC	2 cut(s)	1382, 2566
BalI	TGGCCA	3 cut(s)	1203, 1703, 2003
BanI	GGYRCC	4 cut(s)	139, 1427, 1458, 3068
BanII	GRGCYC	3 cut(s)	45, 230, 1486
BbsI	GAAGAC	3 cut(s)	1134, 1594, 3509
Bbv12I	GWGCWC	4 cut(s)	45, 1382, 2077, 2380
BbvI	GCAGC	8 cut(s)	1064, 1306, 1603, 2209, 2240, 2250, 2315, 2614
BcgI	CGANNNNNNTGC	4 cut(s)	1902, 1936, 2176, 2210
BciT130I	CCWGG	6 cut(s)	361, 1274, 1705, 2754, 3075, 3335
BclI	TGATCA	1 cut(s)	2458
BcnI	CCSGG	1 cut(s)	84
BcoDI	GTCTC	2 cut(s)	1998, 3032
BcuI	ACTAGT	1 cut(s)	605
BfmI	CTRYAG	5 cut(s)	583, 1369, 1929, 2823, 3050
BfoI	RGCGCY	3 cut(s)	106, 143, 2170
BglII	AGATCT	3 cut(s)	125, 464, 1897
BmcAI	AGTACT	2 cut(s)	643, 2041
Bme1390I	CCNGG	7 cut(s)	84, 361, 1274, 1705, 2754, 3075, 3335
BmeT110I	CYCGRG	1 cut(s)	3292
BmrFI	CCNGG	7 cut(s)	84, 361, 1274, 1705, 2754, 3075, 3335
BmrI	ACTGGG	1 cut(s)	999
BmsI	GCATC	5 cut(s)	390, 923, 1505, 1741, 1786
BmuI	ACTGGG	1 cut(s)	999
BoxI	GACNNNNGTC	2 cut(s)	880, 2226
BpiI	GAAGAC	3 cut(s)	1134, 1594, 3509
BpmI	CTGGAG	2 cut(s)	1125, 2202
Bpu10I	CCTNAGC	2 cut(s)	506, 2150
Bpu14I	TTCGAA	1 cut(s)	55
BpuEI	CTTGAG	2 cut(s)	364, 3241
BpuMI	CCSGG	1 cut(s)	84
Bsa29I	ATCGAT	1 cut(s)	3155
BsaHI	GRCGYC	2 cut(s)	140, 3400
BsaJI	CCNNGG	7 cut(s)	83, 349, 1273, 1282, 1980, 3073, 3334
BsaXI	ACNNNNNCTCC	2 cut(s)	995, 1025
Bsc4I	CCNNNNNNNGG	8 cut(s)	82, 1554, 1710, 2404, 2495, 3121, 3125, 3446
Bse118I	RCCGGY	2 cut(s)	142, 387
Bse1I	ACTGG	6 cut(s)	224, 376, 994, 1294, 1816, 2786
Bse21I	CCTNAGG	1 cut(s)	3120
Bse3DI	GCAATG	7 cut(s)	491, 941, 1920, 2022, 2731, 2959, 3191
BseBI	CCWGG	6 cut(s)	361, 1274, 1705, 2754, 3075, 3335
BseCI	ATCGAT	1 cut(s)	3155
BseDI	CCNNGG	7 cut(s)	83, 349, 1273, 1282, 1980, 3073, 3334
BseLI	CCNNNNNNNGG	8 cut(s)	82, 1554, 1710, 2404, 2495, 3121, 3125, 3446
BseMI	GCAATG	7 cut(s)	491, 941, 1920, 2022, 2731, 2959, 3191
BseMII	CTCAG	7 cut(s)	459, 1103, 2009, 2256, 2731, 3134, 3194
BseNI	ACTGG	6 cut(s)	224, 376, 994, 1294, 1816, 2786
BseRI	GAGGAG	5 cut(s)	284, 287, 984, 1009, 3375
BseSI	GKGCMC	2 cut(s)	1382, 2566
BseXI	GCAGC	8 cut(s)	1064, 1306, 1603, 2209, 2240, 2250, 2315, 2614
BseYI	CCCAGC	1 cut(s)	1828
Bsh1236I	CGCG	3 cut(s)	69, 78, 3024
Bsh1285I	CGRYCG	1 cut(s)	392
BshFI	GGCC	7 cut(s)	62, 523, 530, 1203, 1703, 1949, 2003
BshNI	GGYRCC	4 cut(s)	139, 1427, 1458, 3068
BshVI	ATCGAT	1 cut(s)	3155
BsiEI	CGRYCG	1 cut(s)	392
BsiHKAI	GWGCWC	4 cut(s)	45, 1382, 2077, 2380
BsiHKCI	CYCGRG	1 cut(s)	3292
BsiSI	CCGG	5 cut(s)	83, 143, 388, 406, 527
BslFI	GGGAC	5 cut(s)	100, 1290, 2920, 3274, 3439
BslI	CCNNNNNNNGG	8 cut(s)	82, 1554, 1710, 2404, 2495, 3121, 3125, 3446
BsmAI	GTCTC	2 cut(s)	1998, 3032
BsmFI	GGGAC	5 cut(s)	100, 1290, 2920, 3274, 3439
BsmI	GAATGC	1 cut(s)	1780
BsnI	GGCC	7 cut(s)	62, 523, 530, 1203, 1703, 1949, 2003
BsoBI	CYCGRG	1 cut(s)	3292
Bsp119I	TTCGAA	1 cut(s)	55
Bsp1286I	GDGCHC	7 cut(s)	45, 230, 1382, 1486, 2077, 2380, 2566
Bsp1407I	TGTACA	2 cut(s)	213, 2368
Bsp19I	CCATGG	1 cut(s)	1980
Bsp68I	TCGCGA	1 cut(s)	3024
BspANI	GGCC	7 cut(s)	62, 523, 530, 1203, 1703, 1949, 2003
BspCNI	CTCAG	7 cut(s)	460, 1104, 2008, 2257, 2730, 3133, 3193
BspDI	ATCGAT	1 cut(s)	3155
BspFNI	CGCG	3 cut(s)	69, 78, 3024
BspMAI	CTGCAG	2 cut(s)	1373, 1933
BspPI	GGATC	6 cut(s)	8, 74, 658, 1416, 1970, 2986
BspQI	GCTCTTC	1 cut(s)	235
BspT104I	TTCGAA	1 cut(s)	55
BspT107I	GGYRCC	4 cut(s)	139, 1427, 1458, 3068
BsrBI	CCGCTC	2 cut(s)	436, 2982
BsrDI	GCAATG	7 cut(s)	491, 941, 1920, 2022, 2731, 2959, 3191
BsrFI	RCCGGY	2 cut(s)	142, 387
BsrGI	TGTACA	2 cut(s)	213, 2368
BsrI	ACTGG	6 cut(s)	224, 376, 994, 1294, 1816, 2786
BssAI	RCCGGY	2 cut(s)	142, 387
BssECI	CCNNGG	7 cut(s)	83, 349, 1273, 1282, 1980, 3073, 3334
BssNI	GRCGYC	2 cut(s)	140, 3400
BssT1I	CCWWGG	1 cut(s)	1980
Bst2UI	CCWGG	6 cut(s)	361, 1274, 1705, 2754, 3075, 3335
Bst4CI	ACNGT	8 cut(s)	646, 1506, 1939, 2039, 2330, 2568, 3233, 3456
Bst6I	CTCTTC	5 cut(s)	235, 255, 577, 1676, 1722
BstACI	GRCGYC	2 cut(s)	140, 3400
BstAPI	GCANNNNNTGC	1 cut(s)	1377
BstAUI	TGTACA	2 cut(s)	213, 2368
BstBI	TTCGAA	1 cut(s)	55
BstC8I	GCNNGC	4 cut(s)	67, 107, 2005, 2814
BstDSI	CCRYGG	3 cut(s)	349, 1282, 1980
BstEII	GGTNACC	1 cut(s)	2330
BstENI	CCTNNNNNAGG	1 cut(s)	3119
BstFNI	CGCG	3 cut(s)	69, 78, 3024
BstH2I	RGCGCY	3 cut(s)	106, 143, 2170
BstHHI	GCGC	5 cut(s)	105, 142, 411, 928, 2169
BstMAI	GTCTC	2 cut(s)	1998, 3032
BstMCI	CGRYCG	1 cut(s)	392
BstMWI	GCNNNNNNNGC	5 cut(s)	75, 137, 502, 1377, 1775
BstNI	CCWGG	6 cut(s)	361, 1274, 1705, 2754, 3075, 3335
BstNSI	RCATGY	3 cut(s)	784, 2117, 2363
BstPAI	GACNNNNGTC	2 cut(s)	880, 2226
BstPI	GGTNACC	1 cut(s)	2330
BstSCI	CCNGG	7 cut(s)	82, 359, 1272, 1703, 2752, 3073, 3333
BstSFI	CTRYAG	5 cut(s)	583, 1369, 1929, 2823, 3050
BstSLI	GKGCMC	2 cut(s)	1382, 2566
BstUI	CGCG	3 cut(s)	69, 78, 3024
BstV1I	GCAGC	8 cut(s)	1064, 1306, 1603, 2209, 2240, 2250, 2315, 2614
BstV2I	GAAGAC	3 cut(s)	1134, 1594, 3509
BstX2I	RGATCY	6 cut(s)	125, 464, 650, 1408, 1897, 2991
BstYI	RGATCY	6 cut(s)	125, 464, 650, 1408, 1897, 2991
Bsu15I	ATCGAT	1 cut(s)	3155
Bsu36I	CCTNAGG	1 cut(s)	3120
BsuRI	GGCC	7 cut(s)	62, 523, 530, 1203, 1703, 1949, 2003
BsuTUI	ATCGAT	1 cut(s)	3155
BtgI	CCRYGG	3 cut(s)	349, 1282, 1980
BtgZI	GCGATG	1 cut(s)	3354
BtsI	GCAGTG	2 cut(s)	1926, 3376
BtsIMutI	CAGTG	8 cut(s)	369, 1380, 1926, 1944, 2214, 2573, 3238, 3376
BtuMI	TCGCGA	1 cut(s)	3024
Cac8I	GCNNGC	4 cut(s)	67, 107, 2005, 2814
CaiI	CAGNNNCTG	1 cut(s)	3115
CfoI	GCGC	5 cut(s)	105, 142, 411, 928, 2169
Cfr10I	RCCGGY	2 cut(s)	142, 387
ClaI	ATCGAT	1 cut(s)	3155
CseI	GACGC	2 cut(s)	58, 2730
Csp6I	GTAC	8 cut(s)	214, 642, 1171, 1428, 1459, 1641, 2040, 2369
CspCI	CAANNNNNGTGG	4 cut(s)	715, 750, 1003, 1038
CviQI	GTAC	8 cut(s)	214, 642, 1171, 1428, 1459, 1641, 2040, 2369
DinI	GGCGCC	1 cut(s)	141
DrdI	GACNNNNNNGTC	1 cut(s)	2914
DseDI	GACNNNNNNGTC	1 cut(s)	2914
EaeI	YGGCCR	3 cut(s)	1201, 1701, 2001
Eam1104I	CTCTTC	5 cut(s)	235, 255, 577, 1676, 1722
EarI	CTCTTC	5 cut(s)	235, 255, 577, 1676, 1722
Ecl136II	GAGCTC	1 cut(s)	43
Eco130I	CCWWGG	1 cut(s)	1980
Eco24I	GRGCYC	3 cut(s)	45, 230, 1486
Eco32I	GATATC	1 cut(s)	416
Eco47III	AGCGCT	1 cut(s)	2168
Eco53kI	GAGCTC	1 cut(s)	43
Eco57I	CTGAAG	3 cut(s)	2141, 2661, 2667
Eco81I	CCTNAGG	1 cut(s)	3120
Eco88I	CYCGRG	1 cut(s)	3292
Eco91I	GGTNACC	1 cut(s)	2330
EcoICRI	GAGCTC	1 cut(s)	43
EcoNI	CCTNNNNNAGG	1 cut(s)	3119
EcoO109I	RGGNCCY	4 cut(s)	1277, 2907, 3112, 3426
EcoO65I	GGTNACC	1 cut(s)	2330
EcoRI	GAATTC	1 cut(s)	3160
EcoRII	CCWGG	6 cut(s)	359, 1272, 1703, 2752, 3073, 3333
EcoRV	GATATC	1 cut(s)	416
EcoT14I	CCWWGG	1 cut(s)	1980
EcoT22I	ATGCAT	1 cut(s)	1756
EcoT38I	GRGCYC	3 cut(s)	45, 230, 1486
EgeI	GGCGCC	1 cut(s)	141
EheI	GGCGCC	1 cut(s)	141
ErhI	CCWWGG	1 cut(s)	1980
FalI	AAGNNNNNCTT	2 cut(s)	194, 226
FaqI	GGGAC	5 cut(s)	100, 1290, 2920, 3274, 3439
FauI	CCCGC	3 cut(s)	74, 1961, 2020
FauNDI	CATATG	1 cut(s)	2337
FbaI	TGATCA	1 cut(s)	2458
FblI	GTMKAC	2 cut(s)	602, 3451
FriOI	GRGCYC	3 cut(s)	45, 230, 1486
FspI	TGCGCA	1 cut(s)	927
GlaI	GCGC	5 cut(s)	104, 141, 410, 927, 2168
GsaI	CCCAGC	1 cut(s)	1832
GsuI	CTGGAG	2 cut(s)	1125, 2202
HaeII	RGCGCY	3 cut(s)	106, 143, 2170
HaeIII	GGCC	7 cut(s)	62, 523, 530, 1203, 1703, 1949, 2003
HapII	CCGG	5 cut(s)	83, 143, 388, 406, 527
HgaI	GACGC	2 cut(s)	58, 2730
HhaI	GCGC	5 cut(s)	105, 142, 411, 928, 2169
Hin1I	GRCGYC	2 cut(s)	140, 3400
Hin6I	GCGC	5 cut(s)	103, 140, 409, 926, 2167
HinP1I	GCGC	5 cut(s)	103, 140, 409, 926, 2167
HincII	GTYRAC	2 cut(s)	603, 2919
HindII	GTYRAC	2 cut(s)	603, 2919
HindIII	AAGCTT	3 cut(s)	306, 503, 1041
HpaII	CCGG	5 cut(s)	83, 143, 388, 406, 527
HphI	GGTGA	4 cut(s)	37, 971, 2324, 3323
Hpy99I	CGWCG	4 cut(s)	244, 879, 889, 3405
HpyAV	CCTTC	9 cut(s)	159, 196, 217, 813, 1085, 1554, 2223, 2795, 3232
HpyCH4III	ACNGT	8 cut(s)	646, 1506, 1939, 2039, 2330, 2568, 3233, 3456
HpyCH4IV	ACGT	1 cut(s)	3400
HpyF10VI	GCNNNNNNNGC	5 cut(s)	75, 137, 502, 1377, 1775
HpySE526I	ACGT	1 cut(s)	3400
Hsp92I	GRCGYC	2 cut(s)	140, 3400
HspAI	GCGC	5 cut(s)	103, 140, 409, 926, 2167
KasI	GGCGCC	1 cut(s)	139
KflI	GGGWCCC	3 cut(s)	1277, 2907, 3426
KpnI	GGTACC	2 cut(s)	1431, 1462
Ksp22I	TGATCA	1 cut(s)	2458
LguI	GCTCTTC	1 cut(s)	235
LmnI	GCTCC	6 cut(s)	408, 803, 1261, 1345, 1489, 2477
Lsp1109I	GCAGC	8 cut(s)	1064, 1306, 1603, 2209, 2240, 2250, 2315, 2614
LweI	GCATC	5 cut(s)	390, 923, 1505, 1741, 1786
MaeII	ACGT	1 cut(s)	3400
MaeIII	GTNAC	6 cut(s)	365, 1500, 1532, 1890, 2228, 2330
MbiI	CCGCTC	2 cut(s)	436, 2982
MfeI	CAATTG	1 cut(s)	2884
MflI	RGATCY	6 cut(s)	125, 464, 650, 1408, 1897, 2991
MhlI	GDGCHC	7 cut(s)	45, 230, 1382, 1486, 2077, 2380, 2566
MlsI	TGGCCA	3 cut(s)	1203, 1703, 2003
MluNI	TGGCCA	3 cut(s)	1203, 1703, 2003
Mly113I	GGCGCC	1 cut(s)	140
MlyI	GAGTC	6 cut(s)	563, 757, 2054, 2182, 2862, 3094
MmeI	TCCRAC	4 cut(s)	628, 1791, 2418, 3345
Mox20I	TGGCCA	3 cut(s)	1203, 1703, 2003
Mph1103I	ATGCAT	1 cut(s)	1756
MroXI	GAANNNNTTC	2 cut(s)	206, 2712
MscI	TGGCCA	3 cut(s)	1203, 1703, 2003
MseI	TTAA	6 cut(s)	1569, 1683, 2009, 2108, 2549, 3200
MslI	CAYNNNNRTG	1 cut(s)	2616
Msp20I	TGGCCA	3 cut(s)	1203, 1703, 2003
MspI	CCGG	5 cut(s)	83, 143, 388, 406, 527
MspR9I	CCNGG	7 cut(s)	84, 361, 1274, 1705, 2754, 3075, 3335
MunI	CAATTG	1 cut(s)	2884
Mva1269I	GAATGC	1 cut(s)	1780
MvaI	CCWGG	6 cut(s)	361, 1274, 1705, 2754, 3075, 3335
MvnI	CGCG	3 cut(s)	69, 78, 3024
MwoI	GCNNNNNNNGC	5 cut(s)	75, 137, 502, 1377, 1775
NarI	GGCGCC	1 cut(s)	140
NciI	CCSGG	1 cut(s)	84
NcoI	CCATGG	1 cut(s)	1980
NdeI	CATATG	1 cut(s)	2337
NmuCI	GTSAC	5 cut(s)	365, 1500, 1532, 2228, 2330
NruI	TCGCGA	1 cut(s)	3024
NsbI	TGCGCA	1 cut(s)	927
NsiI	ATGCAT	1 cut(s)	1756
NspI	RCATGY	3 cut(s)	784, 2117, 2363
NspV	TTCGAA	1 cut(s)	55
PciSI	GCTCTTC	1 cut(s)	235
PctI	GAATGC	1 cut(s)	1780
PdmI	GAANNNNTTC	2 cut(s)	206, 2712
PflMI	CCANNNNNTGG	1 cut(s)	1554
PfoI	TCCNGGA	1 cut(s)	359
PleI	GAGTC	6 cut(s)	563, 757, 2054, 2182, 2861, 3093
PluTI	GGCGCC	1 cut(s)	143
PpsI	GAGTC	6 cut(s)	563, 757, 2054, 2182, 2861, 3093
PpuMI	RGGWCCY	4 cut(s)	1277, 2907, 3112, 3426
PshAI	GACNNNNGTC	2 cut(s)	880, 2226
PsiI	TTATAA	3 cut(s)	615, 1229, 3390
Psp124BI	GAGCTC	1 cut(s)	45
Psp5II	RGGWCCY	4 cut(s)	1277, 2907, 3112, 3426
Psp6I	CCWGG	6 cut(s)	359, 1272, 1703, 2752, 3073, 3333
PspEI	GGTNACC	1 cut(s)	2330
PspFI	CCCAGC	1 cut(s)	1828
PspGI	CCWGG	6 cut(s)	359, 1272, 1703, 2752, 3073, 3333
PspPPI	RGGWCCY	4 cut(s)	1277, 2907, 3112, 3426
PsrI	GAACNNNNNNTAC	2 cut(s)	2023, 2055
PstI	CTGCAG	2 cut(s)	1373, 1933
PstNI	CAGNNNCTG	1 cut(s)	3115
PsuI	RGATCY	6 cut(s)	125, 464, 650, 1408, 1897, 2991
RruI	TCGCGA	1 cut(s)	3024
RsaI	GTAC	8 cut(s)	215, 643, 1172, 1429, 1460, 1642, 2041, 2370
RsaNI	GTAC	8 cut(s)	214, 642, 1171, 1428, 1459, 1641, 2040, 2369
RseI	CAYNNNNRTG	1 cut(s)	2616
SacI	GAGCTC	1 cut(s)	45
SalI	GTCGAC	1 cut(s)	601
SapI	GCTCTTC	1 cut(s)	235
SaqAI	TTAA	6 cut(s)	1569, 1683, 2009, 2108, 2549, 3200
ScaI	AGTACT	2 cut(s)	643, 2041
SchI	GAGTC	6 cut(s)	563, 757, 2054, 2182, 2862, 3094
ScrFI	CCNGG	7 cut(s)	84, 361, 1274, 1705, 2754, 3075, 3335
SduI	GDGCHC	7 cut(s)	45, 230, 1382, 1486, 2077, 2380, 2566
SfaNI	GCATC	5 cut(s)	390, 923, 1505, 1741, 1786
SfcI	CTRYAG	5 cut(s)	583, 1369, 1929, 2823, 3050
SfoI	GGCGCC	1 cut(s)	141
SfuI	TTCGAA	1 cut(s)	55
SmiMI	CAYNNNNRTG	1 cut(s)	2616
SmlI	CTYRAG	2 cut(s)	343, 3220
SmoI	CTYRAG	2 cut(s)	343, 3220
SpeI	ACTAGT	1 cut(s)	605
SspDI	GGCGCC	1 cut(s)	139
SspI	AATATT	2 cut(s)	1210, 1574
SstI	GAGCTC	1 cut(s)	45
StyD4I	CCNGG	7 cut(s)	82, 359, 1272, 1703, 2752, 3073, 3333
StyI	CCWWGG	1 cut(s)	1980
TaaI	ACNGT	8 cut(s)	646, 1506, 1939, 2039, 2330, 2568, 3233, 3456
TaiI	ACGT	1 cut(s)	3403
TaqI	TCGA	7 cut(s)	55, 242, 533, 602, 1792, 2799, 3155
TaqII	GACCGA	1 cut(s)	2102
TatI	WGTACW	5 cut(s)	213, 641, 1170, 2039, 2368
TauI	GCSGC	8 cut(s)	65, 134, 137, 140, 189, 192, 323, 436
Tru1I	TTAA	6 cut(s)	1569, 1683, 2009, 2108, 2549, 3200
Tru9I	TTAA	6 cut(s)	1569, 1683, 2009, 2108, 2549, 3200
TscAI	CASTG	8 cut(s)	376, 1387, 1933, 1944, 2214, 2573, 3238, 3376
TseFI	GTSAC	5 cut(s)	365, 1500, 1532, 2228, 2330
TseI	GCWGC	8 cut(s)	1077, 1319, 1591, 2197, 2238, 2253, 2303, 2602
Tsp45I	GTSAC	5 cut(s)	365, 1500, 1532, 2228, 2330
TspGWI	ACGGA	2 cut(s)	366, 1078
TspRI	CASTG	8 cut(s)	376, 1387, 1933, 1944, 2214, 2573, 3238, 3376
Van91I	CCANNNNNTGG	1 cut(s)	1554
VneI	GTGCAC	1 cut(s)	1378
XagI	CCTNNNNNAGG	1 cut(s)	3119
XapI	RAATTY	4 cut(s)	1311, 1839, 2698, 3160
XbaI	TCTAGA	3 cut(s)	122, 917, 2666
XceI	RCATGY	3 cut(s)	784, 2117, 2363
XcmI	CCANNNNNNNNNTGG	5 cut(s)	1280, 1555, 1957, 2011, 2753
XmiI	GTMKAC	2 cut(s)	602, 3451
XmnI	GAANNNNTTC	2 cut(s)	206, 2712
ZraI	GACGTC	1 cut(s)	3401
ZrmI	AGTACT	2 cut(s)	643, 2041
Zsp2I	ATGCAT	1 cut(s)	1756

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.