MD09G1059300.v1.1

Plant protein of unknown function (DUF863)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0003069
Physical Location & Seq
Forward (+)
3957138 .. 3959786
2649 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1059300.v1.1.491

Sequence Viewer

Length: 1818 bp
ATGTATTTCCCGGGATATTATTCCATACAGAATCTAAGCAGCGCTGTCAGCCATGGGAGCTGGTCCCTACTCCACGAGAACAGAAACTTGAAGAATGGGCAACAATATGAAGTATTCTTGACAAGGCCAGTTACTGATGGATTTCACGGATGCGATAAGGAACAATTGAGGCAAACTATACTGAAGCATGAGTCAATATTCAAGCATCAGCTAAATGAACTCCACCATCTTTACGAAAGGCAAGAGGACCTGATGAACGAGATGAGAAGCAAAGAGCTGCTCAAACATAAAAAAGCAACAGGGGTATCACAATCAACTTTCTCATCATCCGGCTTTCCAACTGGAGGCTATACAAAGAGTTGGCATCTTTCTAATTTGCCCATGGTGGATTCCAGTTATGGTAGACGATCTACATTGAGTACTTGTATCAGCCAATCTCCTTGTGATTCTATTGGAAAAACCCTGCAAAACAGCGGTGTCCTCAGCCAAAGTAGAGTAGGGATGAAGGACCATGAATCTTTGGATTCCAGAGGGAAGAAGCCTCGAAGAAAATTGTTCAATCTTGAACTTCCAGCCGAGGAATGCCTCAGTGACGAAGACGAACCAGATGGAGGTTTGGGGTTGGGAACAGACAAATCTGGCTGTAACAATAATGCGTCGAGCTCTGATTTGCATTTGGTAAGAAGCAATGGTTTGACTGATTTGAATGAACCTATTCAGATGGACAAAGTGTCTGCTTCAACTTCTGTCGTTATGTCTGGCAATCACCTGAGCTCCAAAGAGGAGATAGAAAGGCAGGTTTTATCTGCAAATGCATACAAAGGGGTGTGGCCCTTTGCAAAGAAATTCCCTGAAAACCCACAAGCCGGGAAGGATGGAAAAGTTCATGATCTGCATTCAAAGAATGAATTCCAAAAAGAATGGTCAACAAATGCTCTCAAAGCTGAGCAAACGACAATTTCTTATGGTGCAAGTTTTAGTCTTCAAGATTTTAATAAGCCATGTGAATCATCAGAACCTGAAGCTAGGATAGCCTGTGAGCCTGCTAAGCTTTTCACATCTGATCAGGATAAAATGGAGAAACGAATAAAGAGGACACTTTTTGGTATAAAAATCTCTGAAAGCGATGTAAGTTCATCTGTTATGGCTTTGCAATCTGATGCAGCCATTTCCCAGTCATCTTCCAATTGGACAAAACCTCCATGTAGCTTGAGCCAGAACTTAACACCAATTCAAGTAAGCACTTCCGTTAATACCTCAACACTATCTAATAGGGCCTTAATCGTGATGCCTGGAAGCAGCGAAGTTATCAGGGACAGATTACTTCTAGATTGTAATTCAATACCATCAACTCCCGGTCTTAAAGATGAAGTGTCACACCAAAGCGGTTTTTTCTTTCGTGCGAAGTTGGAGGTCAATGAAGTACAGGCTTGTCACCCCTCAATGAGCCTTGTCCACCAGGGGAACCATTCAAGTTTGGGTCACCGGGTGGATGTGTCTGCAATTGATGTCAACAATGTTCCTCCGAATGAATATCTGCATGGAGGAACTTGTGGATATTTCTCTTTAGGGGCTAAGGTACCAGAAAGCCGACAAGGAAGATTGCATTGGCTTGAAGCAGTATCACTTTGCAATGGTGAATCTTCCAAAGAAAGTGAAGATTCATCTTCATTAATGCATGGCCATGATGCTGAAAATGGCTACTCTGCTAGAATTGCTTTTGATAAGAGTGATATGGTTCAGGACCCTGTGTCATGTACGTGTAGAGAGCAACTGATTGCACACGGTCTGGTTCTAGACAAGGGATTATCAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF863

Protein Analysis

606

Amino Acids

66.68

Weight (kDa)

6.19

Isoelectric Point (pI)

49.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF863 PF05904 135 - 483 1.2e-16 Plant protein of unknown function (DUF863)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 787
Acc65I GGTACC 1 cut(s) 1579
AccB1I GGYRCC 1 cut(s) 1579
AccI GTMKAC 1 cut(s) 403
AciI CCGC 2 cut(s) 474, 1386
AcoI YGGCCR 1 cut(s) 1681
AcsI RAATTY 2 cut(s) 845, 908
AcuI CTGAAG 2 cut(s) 203, 1041
AdeI CACNNNGTG 1 cut(s) 1489
AfaI GTAC 4 cut(s) 421, 1425, 1581, 1759
AfeI AGCGCT 1 cut(s) 43
AfiI CCNNNNNNNGG 3 cut(s) 344, 611, 866
AflIII ACRYGT 1 cut(s) 1760
AhdI GACNNNNNGTC 2 cut(s) 730, 1750
AjnI CCWGG 2 cut(s) 1291, 1458
AjuI GAANNNNNNNTTGG 2 cut(s) 1591, 1623
AluBI AGCT 9 cut(s) 60, 211, 277, 663, 774, 944, 1025, 1051, 1209
AluI AGCT 9 cut(s) 60, 211, 277, 663, 774, 944, 1025, 1051, 1209
Alw21I GWGCWC 2 cut(s) 665, 776
AlwNI CAGNNNCTG 2 cut(s) 134, 1019
Ama87I CYCGRG 1 cut(s) 10
Aor51HI AGCGCT 1 cut(s) 43
AoxI GGCC 4 cut(s) 125, 830, 1275, 1681
ApeKI GCWGC 4 cut(s) 39, 277, 1163, 1299
ApoI RAATTY 2 cut(s) 845, 908
AseI ATTAAT 1 cut(s) 1673
Asp700I GAANNNNTTC 2 cut(s) 714, 908
Asp718I GGTACC 1 cut(s) 1579
AspLEI GCGC 1 cut(s) 44
AspS9I GGNCC 6 cut(s) 63, 247, 508, 831, 1275, 1744
AsuC2I CCSGG 5 cut(s) 11, 12, 868, 1356, 1487
AsuHPI GGTGA 4 cut(s) 758, 1427, 1475, 1649
AvaI CYCGRG 1 cut(s) 10
AvaII GGWCC 4 cut(s) 63, 247, 508, 1744
BaeI ACNNNNGTAYC 2 cut(s) 288, 321
BalI TGGCCA 1 cut(s) 1683
BanI GGYRCC 1 cut(s) 1579
BanII GRGCYC 2 cut(s) 665, 776
BauI CACGAG 1 cut(s) 74
BbsI GAAGAC 2 cut(s) 603, 974
Bbv12I GWGCWC 2 cut(s) 665, 776
BbvCI CCTCAGC 1 cut(s) 482
BbvI GCAGC 4 cut(s) 51, 264, 1175, 1311
BccI CCATC 6 cut(s) 131, 234, 602, 715, 869, 1354
BciT130I CCWGG 2 cut(s) 1293, 1460
BclI TGATCA 1 cut(s) 1063
BcnI CCSGG 5 cut(s) 11, 12, 868, 1356, 1487
BfaI CTAG 4 cut(s) 1026, 1328, 1710, 1796
BfoI RGCGCY 1 cut(s) 45
BfuAI ACCTGC 1 cut(s) 787
BisI GCNGC 4 cut(s) 40, 278, 1164, 1300
BlpI GCTNAGC 2 cut(s) 945, 1047
BlsI GCNGC 4 cut(s) 41, 279, 1165, 1301
BmcAI AGTACT 1 cut(s) 421
Bme1390I CCNGG 7 cut(s) 11, 12, 868, 1293, 1356, 1460, 1487
Bme18I GGWCC 4 cut(s) 63, 247, 508, 1744
BmeRI GACNNNNNGTC 2 cut(s) 730, 1750
BmeT110I CYCGRG 1 cut(s) 10
BmgT120I GGNCC 6 cut(s) 63, 247, 508, 831, 1275, 1744
BmiI GGNNCC 4 cut(s) 65, 1466, 1581, 1746
BmrFI CCNGG 7 cut(s) 11, 12, 868, 1293, 1356, 1460, 1487
BmrI ACTGGG 1 cut(s) 1168
BmsI GCATC 6 cut(s) 140, 214, 373, 1150, 1278, 1678
BmuI ACTGGG 1 cut(s) 1168
BpiI GAAGAC 2 cut(s) 603, 974
BpmI CTGGAG 1 cut(s) 363
Bpu10I CCTNAGC 3 cut(s) 482, 770, 1575
Bpu1102I GCTNAGC 2 cut(s) 945, 1047
BpuEI CTTGAG 1 cut(s) 1231
BpuMI CCSGG 5 cut(s) 11, 12, 868, 1356, 1487
BsaAI YACGTR 1 cut(s) 1761
BsaJI CCNNGG 5 cut(s) 10, 52, 381, 576, 1459
BsaXI ACNNNNNCTCC 4 cut(s) 758, 788, 1183, 1213
Bsc4I CCNNNNNNNGG 3 cut(s) 344, 611, 866
Bse1I ACTGG 4 cut(s) 128, 346, 393, 1174
Bse3DI GCAATG 2 cut(s) 694, 1639
BseBI CCWGG 2 cut(s) 1293, 1460
BseDI CCNNGG 5 cut(s) 10, 52, 381, 576, 1459
BseGI GGATG 5 cut(s) 155, 326, 507, 880, 1498
BseLI CCNNNNNNNGG 3 cut(s) 344, 611, 866
BseMI GCAATG 2 cut(s) 694, 1639
BseMII CTCAG 4 cut(s) 496, 601, 761, 936
BseNI ACTGG 4 cut(s) 128, 346, 393, 1174
BseRI GAGGAG 1 cut(s) 797
BseXI GCAGC 4 cut(s) 51, 264, 1175, 1311
BshFI GGCC 4 cut(s) 127, 832, 1277, 1683
BshNI GGYRCC 1 cut(s) 1579
BsiHKAI GWGCWC 2 cut(s) 665, 776
BsiHKCI CYCGRG 1 cut(s) 10
BsiSI CCGG 5 cut(s) 11, 330, 867, 1356, 1486
BslFI GGGAC 2 cut(s) 49, 1328
BslI CCNNNNNNNGG 3 cut(s) 344, 611, 866
BsmFI GGGAC 2 cut(s) 49, 1328
BsmI GAATGC 2 cut(s) 587, 895
BsnI GGCC 4 cut(s) 127, 832, 1277, 1683
BsoBI CYCGRG 1 cut(s) 10
Bsp1286I GDGCHC 2 cut(s) 665, 776
Bsp143I GATC 3 cut(s) 407, 889, 1063
Bsp1720I GCTNAGC 2 cut(s) 945, 1047
Bsp19I CCATGG 2 cut(s) 52, 381
BspACI CCGC 2 cut(s) 474, 1386
BspANI GGCC 4 cut(s) 127, 832, 1277, 1683
BspCNI CTCAG 4 cut(s) 495, 600, 762, 937
BspHI TCATGA 1 cut(s) 886
BspLI GGNNCC 4 cut(s) 65, 1466, 1581, 1746
BspMI ACCTGC 1 cut(s) 787
BspT107I GGYRCC 1 cut(s) 1579
BsrDI GCAATG 2 cut(s) 694, 1639
BsrI ACTGG 4 cut(s) 128, 346, 393, 1174
BssECI CCNNGG 5 cut(s) 10, 52, 381, 576, 1459
BssMI GATC 3 cut(s) 407, 889, 1063
BssSI CACGAG 1 cut(s) 74
BssT1I CCWWGG 2 cut(s) 52, 381
Bst2BI CACGAG 1 cut(s) 74
Bst2UI CCWGG 2 cut(s) 1293, 1460
Bst4CI ACNGT 1 cut(s) 1787
BstBAI YACGTR 1 cut(s) 1761
BstC8I GCNNGC 1 cut(s) 1044
BstDEI CTNAG 7 cut(s) 35, 482, 587, 770, 945, 1047, 1575
BstDSI CCRYGG 2 cut(s) 52, 381
BstEII GGTNACC 1 cut(s) 1481
BstF5I GGATG 5 cut(s) 155, 326, 507, 880, 1498
BstH2I RGCGCY 1 cut(s) 45
BstHHI GCGC 1 cut(s) 44
BstKTI GATC 3 cut(s) 410, 892, 1066
BstMBI GATC 3 cut(s) 407, 889, 1063
BstMWI GCNNNNNNNGC 6 cut(s) 48, 57, 941, 1031, 1048, 1715
BstNI CCWGG 2 cut(s) 1293, 1460
BstPI GGTNACC 1 cut(s) 1481
BstSCI CCNGG 7 cut(s) 9, 10, 866, 1291, 1354, 1458, 1485
BstV1I GCAGC 4 cut(s) 51, 264, 1175, 1311
BstV2I GAAGAC 2 cut(s) 603, 974
BsuRI GGCC 4 cut(s) 127, 832, 1277, 1683
BtgI CCRYGG 2 cut(s) 52, 381
BtgZI GCGATG 1 cut(s) 1140
BtsCI GGATG 5 cut(s) 155, 326, 507, 880, 1498
BtsIMutI CAGTG 1 cut(s) 595
BveI ACCTGC 1 cut(s) 787
Cac8I GCNNGC 1 cut(s) 1044
CaiI CAGNNNCTG 2 cut(s) 134, 1019
CciI TCATGA 1 cut(s) 886
CfoI GCGC 1 cut(s) 44
Cfr13I GGNCC 6 cut(s) 63, 247, 508, 831, 1275, 1744
Cfr9I CCCGGG 1 cut(s) 10
CseI GACGC 1 cut(s) 645
Csp6I GTAC 4 cut(s) 420, 1424, 1580, 1758
CviQI GTAC 4 cut(s) 420, 1424, 1580, 1758
DdeI CTNAG 7 cut(s) 35, 482, 587, 770, 945, 1047, 1575
DpnI GATC 3 cut(s) 409, 891, 1065
DpnII GATC 3 cut(s) 407, 889, 1063
DraIII CACNNNGTG 1 cut(s) 1489
DriI GACNNNNNGTC 2 cut(s) 730, 1750
EaeI YGGCCR 1 cut(s) 1681
Eam1105I GACNNNNNGTC 2 cut(s) 730, 1750
Ecl136II GAGCTC 2 cut(s) 663, 774
Eco130I CCWWGG 2 cut(s) 52, 381
Eco24I GRGCYC 2 cut(s) 665, 776
Eco47I GGWCC 4 cut(s) 63, 247, 508, 1744
Eco47III AGCGCT 1 cut(s) 43
Eco53kI GAGCTC 2 cut(s) 663, 774
Eco57I CTGAAG 2 cut(s) 203, 1041
Eco88I CYCGRG 1 cut(s) 10
Eco91I GGTNACC 1 cut(s) 1481
EcoICRI GAGCTC 2 cut(s) 663, 774
EcoO109I RGGNCCY 3 cut(s) 247, 1275, 1744
EcoO65I GGTNACC 1 cut(s) 1481
EcoRI GAATTC 1 cut(s) 908
EcoRII CCWGG 2 cut(s) 1291, 1458
EcoT14I CCWWGG 2 cut(s) 52, 381
EcoT22I ATGCAT 2 cut(s) 817, 1680
EcoT38I GRGCYC 2 cut(s) 665, 776
ErhI CCWWGG 2 cut(s) 52, 381
FaqI GGGAC 2 cut(s) 49, 1328
FbaI TGATCA 1 cut(s) 1063
FblI GTMKAC 1 cut(s) 403
Fnu4HI GCNGC 4 cut(s) 40, 278, 1164, 1300
FokI GGATG 5 cut(s) 162, 313, 514, 887, 1505
FriOI GRGCYC 2 cut(s) 665, 776
Fsp4HI GCNGC 4 cut(s) 40, 278, 1164, 1300
FspBI CTAG 4 cut(s) 1026, 1328, 1710, 1796
GlaI GCGC 1 cut(s) 43
GluI GCNGC 4 cut(s) 40, 278, 1164, 1300
GsuI CTGGAG 1 cut(s) 363
HaeII RGCGCY 1 cut(s) 45
HaeIII GGCC 4 cut(s) 127, 832, 1277, 1683
HapII CCGG 5 cut(s) 11, 330, 867, 1356, 1486
HgaI GACGC 1 cut(s) 645
HhaI GCGC 1 cut(s) 44
Hin6I GCGC 1 cut(s) 42
HinP1I GCGC 1 cut(s) 42
HincII GTYRAC 2 cut(s) 927, 1513
HindII GTYRAC 2 cut(s) 927, 1513
HindIII AAGCTT 1 cut(s) 1049
HinfI GANTC 9 cut(s) 31, 191, 389, 446, 515, 524, 1007, 1640, 1661
HpaII CCGG 5 cut(s) 11, 330, 867, 1356, 1486
HphI GGTGA 4 cut(s) 758, 1427, 1475, 1649
Hpy166II GTNNAC 4 cut(s) 404, 927, 1456, 1513
Hpy188I TCNGA 7 cut(s) 667, 720, 1015, 1063, 1120, 1159, 1527
Hpy8I GTNNAC 4 cut(s) 404, 927, 1456, 1513
Hpy99I CGWCG 1 cut(s) 661
HpyAV CCTTC 2 cut(s) 499, 865
HpyCH4III ACNGT 1 cut(s) 1787
HpyCH4IV ACGT 1 cut(s) 1760
HpyF10VI GCNNNNNNNGC 6 cut(s) 48, 57, 941, 1031, 1048, 1715
HpyF3I CTNAG 7 cut(s) 35, 482, 587, 770, 945, 1047, 1575
HpySE526I ACGT 1 cut(s) 1760
HspAI GCGC 1 cut(s) 42
KpnI GGTACC 1 cut(s) 1583
Ksp22I TGATCA 1 cut(s) 1063
Kzo9I GATC 3 cut(s) 407, 889, 1063
LmnI GCTCC 2 cut(s) 57, 779
Lsp1109I GCAGC 4 cut(s) 51, 264, 1175, 1311
LweI GCATC 6 cut(s) 140, 214, 373, 1150, 1278, 1678
MaeI CTAG 4 cut(s) 1026, 1328, 1710, 1796
MaeII ACGT 1 cut(s) 1760
MaeIII GTNAC 6 cut(s) 130, 590, 644, 1374, 1433, 1481
MalI GATC 3 cut(s) 409, 891, 1065
MboI GATC 3 cut(s) 407, 889, 1063
MfeI CAATTG 3 cut(s) 164, 1186, 1503
MhlI GDGCHC 2 cut(s) 665, 776
MlsI TGGCCA 1 cut(s) 1683
MluNI TGGCCA 1 cut(s) 1683
MlyI GAGTC 1 cut(s) 200
MmeI TCCRAC 2 cut(s) 362, 1389
Mox20I TGGCCA 1 cut(s) 1683
Mph1103I ATGCAT 2 cut(s) 817, 1680
MroXI GAANNNNTTC 2 cut(s) 714, 908
MscI TGGCCA 1 cut(s) 1683
MseI TTAA 6 cut(s) 993, 1223, 1251, 1280, 1362, 1673
MslI CAYNNNNRTG 2 cut(s) 1683, 1759
Msp20I TGGCCA 1 cut(s) 1683
MspA1I CMGCKG 1 cut(s) 474
MspI CCGG 5 cut(s) 11, 330, 867, 1356, 1486
MspR9I CCNGG 7 cut(s) 11, 12, 868, 1293, 1356, 1460, 1487
MunI CAATTG 3 cut(s) 164, 1186, 1503
Mva1269I GAATGC 2 cut(s) 587, 895
MvaI CCWGG 2 cut(s) 1293, 1460
MwoI GCNNNNNNNGC 6 cut(s) 48, 57, 941, 1031, 1048, 1715
NciI CCSGG 5 cut(s) 11, 12, 868, 1356, 1487
NcoI CCATGG 2 cut(s) 52, 381
NdeII GATC 3 cut(s) 407, 889, 1063
NlaIV GGNNCC 4 cut(s) 65, 1466, 1581, 1746
NmeAIII GCCGAG 1 cut(s) 601
NmuCI GTSAC 4 cut(s) 590, 1374, 1433, 1481
NsiI ATGCAT 2 cut(s) 817, 1680
PagI TCATGA 1 cut(s) 886
PctI GAATGC 2 cut(s) 587, 895
PdmI GAANNNNTTC 2 cut(s) 714, 908
PfeI GAWTC 8 cut(s) 31, 389, 446, 515, 524, 1007, 1640, 1661
PkrI GCNGC 4 cut(s) 41, 279, 1165, 1301
PleI GAGTC 1 cut(s) 199
PpsI GAGTC 1 cut(s) 199
Ppu21I YACGTR 1 cut(s) 1761
PpuMI RGGWCCY 2 cut(s) 247, 1744
PshBI ATTAAT 1 cut(s) 1673
Psp124BI GAGCTC 2 cut(s) 665, 776
Psp5II RGGWCCY 2 cut(s) 247, 1744
Psp6I CCWGG 2 cut(s) 1291, 1458
PspEI GGTNACC 1 cut(s) 1481
PspGI CCWGG 2 cut(s) 1291, 1458
PspN4I GGNNCC 4 cut(s) 65, 1466, 1581, 1746
PspPI GGNCC 6 cut(s) 63, 247, 508, 831, 1275, 1744
PspPPI RGGWCCY 2 cut(s) 247, 1744
PstNI CAGNNNCTG 2 cut(s) 134, 1019
RsaI GTAC 4 cut(s) 421, 1425, 1581, 1759
RsaNI GTAC 4 cut(s) 420, 1424, 1580, 1758
RseI CAYNNNNRTG 2 cut(s) 1683, 1759
SacI GAGCTC 2 cut(s) 665, 776
SaqAI TTAA 6 cut(s) 993, 1223, 1251, 1280, 1362, 1673
SatI GCNGC 4 cut(s) 40, 278, 1164, 1300
Sau3AI GATC 3 cut(s) 407, 889, 1063
Sau96I GGNCC 6 cut(s) 63, 247, 508, 831, 1275, 1744
ScaI AGTACT 1 cut(s) 421
SchI GAGTC 1 cut(s) 200
ScrFI CCNGG 7 cut(s) 11, 12, 868, 1293, 1356, 1460, 1487
SduI GDGCHC 2 cut(s) 665, 776
SfaNI GCATC 6 cut(s) 140, 214, 373, 1150, 1278, 1678
SinI GGWCC 4 cut(s) 63, 247, 508, 1744
SmaI CCCGGG 1 cut(s) 12
SmiMI CAYNNNNRTG 2 cut(s) 1683, 1759
SmlI CTYRAG 1 cut(s) 1210
SmoI CTYRAG 1 cut(s) 1210
SsiI CCGC 2 cut(s) 474, 1386
SspI AATATT 1 cut(s) 198
SspMI CTAG 4 cut(s) 1026, 1328, 1710, 1796
SstI GAGCTC 2 cut(s) 665, 776
StyD4I CCNGG 7 cut(s) 9, 10, 866, 1291, 1354, 1458, 1485
StyI CCWWGG 2 cut(s) 52, 381
TaaI ACNGT 1 cut(s) 1787
TaiI ACGT 1 cut(s) 1763
TaqI TCGA 2 cut(s) 544, 659
TatI WGTACW 2 cut(s) 419, 1423
TfiI GAWTC 8 cut(s) 31, 389, 446, 515, 524, 1007, 1640, 1661
Tru1I TTAA 6 cut(s) 993, 1223, 1251, 1280, 1362, 1673
Tru9I TTAA 6 cut(s) 993, 1223, 1251, 1280, 1362, 1673
TscAI CASTG 1 cut(s) 595
TseFI GTSAC 4 cut(s) 590, 1374, 1433, 1481
TseI GCWGC 4 cut(s) 39, 277, 1163, 1299
Tsp45I GTSAC 4 cut(s) 590, 1374, 1433, 1481
TspGWI ACGGA 2 cut(s) 162, 1237
TspMI CCCGGG 1 cut(s) 10
TspRI CASTG 1 cut(s) 595
VpaK11BI GGWCC 4 cut(s) 63, 247, 508, 1744
VspI ATTAAT 1 cut(s) 1673
XapI RAATTY 2 cut(s) 845, 908
XbaI TCTAGA 2 cut(s) 1327, 1795
XmaI CCCGGG 1 cut(s) 10
XmiI GTMKAC 1 cut(s) 403
XmnI GAANNNNTTC 2 cut(s) 714, 908
XspI CTAG 4 cut(s) 1026, 1328, 1710, 1796
ZrmI AGTACT 1 cut(s) 421
Zsp2I ATGCAT 2 cut(s) 817, 1680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.