Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0003069

Physical Location & Seq

Genomic Coordinates Forward (+)

4230326 .. 4235068

Gene Structure 4743 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1054900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2565 bp

ATGTATTTCCCGGAATATTGTTCCATACAGAATCTAAGCAGCAATGTCGGCCATGGGAGCTGGTCCCTACTCCACGAGAACACAAACTTGAAGAATGGGCAACAATACGAAGTGTTCTTGACAAGGCCAGTTATTGATGGATTTCACGGACACGATAAGGAGCAACTGAGGCAAACTATTCTGAAGCATGAGACAATTTTCAGGCATCAGCTCAATGAACTCCATCGTCTTTACGAAAGGCAAAAGGACCTGATGAACGAGATCAAAAGCAAAGAGCTTCTTAAACATCAAAAAGTAGCAGGGATATCACAATCAACTTTCTCATCATCCGGCTTTCCAACTGGAGACGACAGAAAGAGTTGGCATATTTCTAATTTAGCCTTGTTGGATTCCAGTTATGGTAGGCAATCTACATCGAGTACCTGTATCAGCCAACCTCCTCGCGATTCTATTGGAAAAACCCTGCAAAACAGCTGTGTCCCCAGCCAAAGTAGAGTAGGGATGAAGGACTACGAATCTTCGGATTCCAGAGTGAAGAAGCCTCGAAGAAGATTGTTCAATCTTGAACTTCCAGCCGATGAATACCTCAGTGATGAAGAAGAACCAGAAGGAGGTTTGGGGTCGGGAACAGACAAATCTGGCTATAACAATAGTGTGTCGAGCTCTGGTTTGCATTTAGTAAGAAGCAATGGTTTGGCTGATCTGAATGAACCTATTCAGATAGACAAAGTGTCTGCCTCAACTTCTGTCGTTATGTCTGGCAACCAACCGAGCTCCAAAGAGGAGATAGAAAGGCAGGTTTTATCTGCAAATGCGTACAAAGGCGTGTGGCCTTTTGCAAAGAAATTCCCTGAAAACCCACAAACAGGGAAGGATGGAAGAGTTGGTGATCTGCATTTAAAGAATGAAATCCAAAAAGAGTGGTCAACAAATGCTCTCAGAGATGAGCAAATAAGAAGTTCTTCTGGCGGAAAGTTTGGTCTTCAAGATTTTAATAAGCCATGTGAATTGTCAGAAAACGAAGCTACGGTAACCTGTGCTCCTTCTGCTAAGCTTTTCACTTCTGATCAGAATAAAACGGAGAAACAAATAAAGAGGACACTTTTTGGTATAGAAATCTCTGAAAGCGATATAAGTTCATCTGCTATGACTTCGCAATCTGGTGCAGCCATTTCCGAGTCATCTCCTAATTGGACAATACCTCCAAGTAGCTTGAGCGAGAACTTAACATCAATTCAAGTAAGCACTTCCGTTAATACCTCCACACAATCTAATAGGGCTTCAATCATGATGCCACAAAGCAGTGAAGTTACCCGGGACAGATTGCTTCTTGACTGTAATTCAATACCGTCAACTCCCAGTCTGAAAGACGAAGTGTCACACCAAAGCAGTTTTTTCTTCCGCACTAAGTTGGAGGCCAATCAATTACAGGCTTGGCACCCCTCAATGAGCCTTGGCTGCCAAGGGAACCATTCAACGTCAGGTCACCGGGTGGATGTCAAACCTGCTATTGATGTGAATGATGTTCCTCCTAATAAATATCTGCATGGAGGAGCTTGTGAACAAAATATAATTTCTCTCAATGGGTTGAGGAACCAAGAAAGCCGACAAGGAAGATTGCATTGGCTTGAAGCACTGCCACTTTGTGATGGCAAATCTTCCAGAGAAATTGAAAGCAGACGCACGTGTAGAGGGCAACTGAAAGCAGGCGGTCTGGTTCTAGACAAGGGATTTTCAAATGAGAATTCTGATCCAAGACACCAAATAGATTTAAATAGATGTTTAACTGAAGAAGAAACTGAAATGACTGCTGCTTCCCCAGTTATGAGGGCAGAAATTGTGACAGATTTGGAGGCACCTCTTGACTCACCCCATGAGGGCTTTATCAGAGTTGCAGCCGAGGCTCTAGTTGCCATCTCATCATCCCAAGCTCCCGATATGCAGGAAAAAGCCACTTGTCGCAATCTGGAGGCTTCAGAAAACAACTCCCTTCTTTGGTTTGCAGAAGTAATTTCTTCACATGATGGAAATCTTGACAATGGAAATGCAGCAGAGGTTAAGCAAACTTCCTGTGATGAAGAGGCTTTTCCCGTTGACATGGATTTTTTTGAGCACATGACATTAAATTTGGTGGAAACCAAAGAAGAGCAGCAATGTTATGTGCCTCCAAATTCAGACAGCCTCACAGAAGAAGCGACATTGTCGAAACGGCCACGAAGAGGCCAATCGAGGAGGGGGAGGCAACAGAAGGATTTCCAGAGAGATGTACTTCCCAGTCTTGCTTCTTTGTCAAAAAATGAGGTGATCGATGATCTTCAAATAATTGAAGGGTTGATTAGGGAATCTGGTGGGAATTGGCAATCAAGTTTGTCAAAAAGAAATTCTGGTAAGGGTGGTAGGGGAAGGGGGAGGAAACGTATGGACACTCCCATACCGTCTACTAATGTGGCTGAAGTTTGCCAGCCCCAGATTGAGCAACCAAAATGCGAGGAGCTGCAGGGACCTGAGGAGAGAAATTTAACTTGTTGGGGTAAGAGGACAAGGCGTCCACCACGGCAAAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF863

Protein Analysis

855

Amino Acids

95.02

Weight (kDa)

5.92

Isoelectric Point (pI)

58.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF863	PF05904	133 - 201	2.1e-07	Plant protein of unknown function (DUF863)
DUF863	PF05904	211 - 482	7.5e-12	Plant protein of unknown function (DUF863)
DUF863	PF05904	622 - 854	3.1e-71	Plant protein of unknown function (DUF863)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0003069)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g47740 FvH4_6g47740 FvH4_6g47740 FvH4_6g47740
malus_domestica	MD09G1059300.v1.1 MD17G1054900.v1.1
prunus_persica	Prupe.3G261200_v2.0.a1
pyrus_communis	pycom08g18570 pycom111g05020 pycom111g05040 pycom111g05060 pycom17g05270 pycom17g05290
rosa_chinensis	RchiOBHm_Chr2g0166861 RchiOBHm_Chr4g0401141 RchiOBHm_Chr5g0037041 RchiOBHm_Chr7g0237831
rosa_laevigata	RLG00000021662 RLG00000021675
rosa_multiflora	Rmu_sc0005344.1_g000006
rosa_roxburghii	Rroxscaffold_2G00084060 Rroxscaffold_2G00084090 Rroxscaffold_2G00084470 Rroxscaffold_4G00327630
rosa_rugosa	Rorug02G0525900 Rorug02G0526000
rosa_samantha	Rh2AG592900 Rh2BG604100 Rh2BG604300 Rh2CG575000 Rh2CG575200 Rh2DG615600 Rh2DG615800 Rh3DG312900 Rh4DG186200 Rh5DG059300 Rh5DG383900
rosa_wichuraiana	Rw2G049350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	787, 1513
AccB1I	GGYRCC	2 cut(s)	1437, 1855
AccI	GTMKAC	1 cut(s)	2438
AccII	CGCG	1 cut(s)	444
AciI	CCGC	3 cut(s)	969, 1402, 1710
AclWI	GGATC	1 cut(s)	1745
AcoI	YGGCCR	2 cut(s)	49, 2210
AcsI	RAATTY	6 cut(s)	845, 1744, 2125, 2170, 2380, 2515
AcuI	CTGAAG	4 cut(s)	203, 1809, 1959, 2472
AcvI	CACGTG	1 cut(s)	1686
AcyI	GRCGYC	1 cut(s)	2545
AdeI	CACNNNGTG	2 cut(s)	1492, 1646
AfaI	GTAC	3 cut(s)	421, 818, 2268
AfiI	CCNNNNNNNGG	5 cut(s)	611, 866, 1877, 1995, 2219
AflIII	ACRYGT	1 cut(s)	1685
AhdI	GACNNNNNGTC	3 cut(s)	730, 1375, 2544
AjuI	GAANNNNNNNTTGG	2 cut(s)	1606, 1638
Alw21I	GWGCWC	4 cut(s)	665, 776, 1042, 2115
Alw26I	GTCTC	2 cut(s)	185, 339
AlwI	GGATC	1 cut(s)	1745
Ama87I	CYCGRG	1 cut(s)	1314
AoxI	GGCC	6 cut(s)	49, 125, 830, 1416, 2210, 2221
ApeKI	GCWGC	8 cut(s)	39, 1166, 1458, 1811, 1895, 2048, 2149, 2494
ApoI	RAATTY	6 cut(s)	845, 1744, 2125, 2170, 2380, 2515
ArsI	GACNNNNNNTTYG	2 cut(s)	2110, 2142
Asp700I	GAANNNNTTC	3 cut(s)	714, 961, 2252
AspS9I	GGNCC	3 cut(s)	63, 247, 2501
AsuC2I	CCSGG	4 cut(s)	11, 1315, 1316, 1490
AsuHPI	GGTGA	4 cut(s)	899, 1478, 1860, 2314
AvaI	CYCGRG	1 cut(s)	1314
AvaII	GGWCC	3 cut(s)	63, 247, 2501
AxyI	CCTNAGG	1 cut(s)	2505
BanI	GGYRCC	2 cut(s)	1437, 1855
BanII	GRGCYC	2 cut(s)	665, 776
BauI	CACGAG	1 cut(s)	74
BbrPI	CACGTG	1 cut(s)	1686
BbsI	GAAGAC	1 cut(s)	974
Bbv12I	GWGCWC	4 cut(s)	665, 776, 1042, 2115
BbvI	GCAGC	8 cut(s)	51, 1178, 1445, 1798, 1907, 2060, 2161, 2481
BccI	CCATC	6 cut(s)	131, 231, 869, 1643, 1922, 2018
BceAI	ACGGC	1 cut(s)	2225
BcgI	CGANNNNNNTGC	2 cut(s)	28, 62
BclI	TGATCA	1 cut(s)	1066
BcnI	CCSGG	4 cut(s)	11, 1315, 1316, 1490
BcoDI	GTCTC	2 cut(s)	185, 339
BfaI	CTAG	2 cut(s)	1721, 1907
BfmI	CTRYAG	1 cut(s)	2495
BfuAI	ACCTGC	2 cut(s)	787, 1513
BisI	GCNGC	8 cut(s)	40, 1167, 1459, 1812, 1896, 2049, 2150, 2495
BlpI	GCTNAGC	1 cut(s)	1050
BlsI	GCNGC	8 cut(s)	41, 1168, 1460, 1813, 1897, 2050, 2151, 2496
Bme1390I	CCNGG	4 cut(s)	11, 1315, 1316, 1490
Bme18I	GGWCC	3 cut(s)	63, 247, 2501
BmeRI	GACNNNNNGTC	3 cut(s)	730, 1375, 2544
BmeT110I	CYCGRG	1 cut(s)	1314
BmgT120I	GGNCC	3 cut(s)	63, 247, 2501
BmiI	GGNNCC	6 cut(s)	65, 1439, 1469, 1595, 1857, 2502
BmrFI	CCNGG	4 cut(s)	11, 1315, 1316, 1490
BmrI	ACTGGG	3 cut(s)	1353, 1814, 2268
BmsI	GCATC	2 cut(s)	214, 1281
BmuI	ACTGGG	3 cut(s)	1353, 1814, 2268
BpiI	GAAGAC	1 cut(s)	974
BpmI	CTGGAG	2 cut(s)	363, 1988
Bpu1102I	GCTNAGC	1 cut(s)	1050
BpuEI	CTTGAG	1 cut(s)	1234
BpuMI	CCSGG	4 cut(s)	11, 1315, 1316, 1490
Bsa29I	ATCGAT	1 cut(s)	2307
BsaAI	YACGTR	1 cut(s)	1686
BsaBI	GATNNNNATC	1 cut(s)	2028
BsaHI	GRCGYC	1 cut(s)	2545
BsaJI	CCNNGG	6 cut(s)	52, 1314, 1453, 1462, 1899, 2552
BsaXI	ACNNNNNCTCC	6 cut(s)	1024, 1054, 1186, 1216, 2401, 2431
Bsc4I	CCNNNNNNNGG	5 cut(s)	611, 866, 1877, 1995, 2219
Bse1I	ACTGG	6 cut(s)	128, 346, 393, 1359, 1820, 2274
Bse21I	CCTNAGG	1 cut(s)	2505
Bse3DI	GCAATG	3 cut(s)	49, 694, 2159
Bse8I	GATNNNNATC	1 cut(s)	2028
BseCI	ATCGAT	1 cut(s)	2307
BseDI	CCNNGG	6 cut(s)	52, 1314, 1453, 1462, 1899, 2552
BseGI	GGATG	5 cut(s)	326, 507, 880, 1501, 1922
BseJI	GATNNNNATC	1 cut(s)	2028
BseLI	CCNNNNNNNGG	5 cut(s)	611, 866, 1877, 1995, 2219
BseMI	GCAATG	3 cut(s)	49, 694, 2159
BseMII	CTCAG	4 cut(s)	158, 601, 952, 2496
BseNI	ACTGG	6 cut(s)	128, 346, 393, 1359, 1820, 2274
BseRI	GAGGAG	6 cut(s)	429, 797, 1566, 2245, 2504, 2522
BseXI	GCAGC	8 cut(s)	51, 1178, 1445, 1798, 1907, 2060, 2161, 2481
BseYI	CCCAGC	1 cut(s)	482
BsgI	GTGCAG	1 cut(s)	1185
Bsh1236I	CGCG	1 cut(s)	444
BshFI	GGCC	6 cut(s)	51, 127, 832, 1418, 2212, 2223
BshNI	GGYRCC	2 cut(s)	1437, 1855
BshVI	ATCGAT	1 cut(s)	2307
BsiHKAI	GWGCWC	4 cut(s)	665, 776, 1042, 2115
BsiHKCI	CYCGRG	1 cut(s)	1314
BsiSI	CCGG	4 cut(s)	11, 330, 1315, 1489
BslFI	GGGAC	4 cut(s)	49, 464, 1331, 2514
BslI	CCNNNNNNNGG	5 cut(s)	611, 866, 1877, 1995, 2219
BsmAI	GTCTC	2 cut(s)	185, 339
BsmBI	CGTCTC	1 cut(s)	339
BsmFI	GGGAC	4 cut(s)	49, 464, 1331, 2514
BsnI	GGCC	6 cut(s)	51, 127, 832, 1418, 2212, 2223
BsoBI	CYCGRG	1 cut(s)	1314
Bsp1286I	GDGCHC	4 cut(s)	665, 776, 1042, 2115
Bsp143I	GATC	7 cut(s)	261, 700, 889, 1066, 1750, 2304, 2311
Bsp1720I	GCTNAGC	1 cut(s)	1050
Bsp19I	CCATGG	1 cut(s)	52
Bsp68I	TCGCGA	1 cut(s)	444
BspACI	CCGC	3 cut(s)	969, 1402, 1710
BspANI	GGCC	6 cut(s)	51, 127, 832, 1418, 2212, 2223
BspCNI	CTCAG	4 cut(s)	159, 600, 951, 2497
BspDI	ATCGAT	1 cut(s)	2307
BspFNI	CGCG	1 cut(s)	444
BspHI	TCATGA	1 cut(s)	1287
BspLI	GGNNCC	6 cut(s)	65, 1439, 1469, 1595, 1857, 2502
BspMAI	CTGCAG	1 cut(s)	2499
BspMI	ACCTGC	2 cut(s)	787, 1513
BspPI	GGATC	1 cut(s)	1745
BspQI	GCTCTTC	1 cut(s)	2139
BspT107I	GGYRCC	2 cut(s)	1437, 1855
BsrDI	GCAATG	3 cut(s)	49, 694, 2159
BsrI	ACTGG	6 cut(s)	128, 346, 393, 1359, 1820, 2274
BssECI	CCNNGG	6 cut(s)	52, 1314, 1453, 1462, 1899, 2552
BssMI	GATC	7 cut(s)	261, 700, 889, 1066, 1750, 2304, 2311
BssNI	GRCGYC	1 cut(s)	2545
BssSI	CACGAG	1 cut(s)	74
BssT1I	CCWWGG	3 cut(s)	52, 1453, 1462
Bst2BI	CACGAG	1 cut(s)	74
Bst4CI	ACNGT	4 cut(s)	1030, 1337, 1350, 2436
Bst6I	CTCTTC	4 cut(s)	874, 2073, 2139, 2212
BstACI	GRCGYC	1 cut(s)	2545
BstBAI	YACGTR	1 cut(s)	1686
BstC8I	GCNNGC	2 cut(s)	1708, 2462
BstDEI	CTNAG	7 cut(s)	35, 167, 587, 938, 1050, 1407, 2505
BstDSI	CCRYGG	2 cut(s)	52, 2552
BstEII	GGTNACC	2 cut(s)	1030, 1484
BstF5I	GGATG	5 cut(s)	326, 507, 880, 1501, 1922
BstFNI	CGCG	1 cut(s)	444
BstKTI	GATC	7 cut(s)	264, 703, 892, 1069, 1753, 2307, 2314
BstMAI	GTCTC	2 cut(s)	185, 339
BstMBI	GATC	7 cut(s)	261, 700, 889, 1066, 1750, 2304, 2311
BstMWI	GCNNNNNNNGC	7 cut(s)	48, 57, 169, 1046, 1458, 1901, 1910
BstPI	GGTNACC	2 cut(s)	1030, 1484
BstSCI	CCNGG	4 cut(s)	9, 1313, 1314, 1488
BstSFI	CTRYAG	1 cut(s)	2495
BstUI	CGCG	1 cut(s)	444
BstV1I	GCAGC	8 cut(s)	51, 1178, 1445, 1798, 1907, 2060, 2161, 2481
BstV2I	GAAGAC	1 cut(s)	974
Bsu15I	ATCGAT	1 cut(s)	2307
Bsu36I	CCTNAGG	1 cut(s)	2505
BsuRI	GGCC	6 cut(s)	51, 127, 832, 1418, 2212, 2223
BsuTUI	ATCGAT	1 cut(s)	2307
BtgI	CCRYGG	2 cut(s)	52, 2552
BtsCI	GGATG	5 cut(s)	326, 507, 880, 1501, 1922
BtsI	GCAGTG	2 cut(s)	1309, 1634
BtsIMutI	CAGTG	3 cut(s)	595, 1309, 1634
BtuMI	TCGCGA	1 cut(s)	444
BveI	ACCTGC	2 cut(s)	787, 1513
Cac8I	GCNNGC	2 cut(s)	1708, 2462
CciI	TCATGA	1 cut(s)	1287
Cfr13I	GGNCC	3 cut(s)	63, 247, 2501
Cfr9I	CCCGGG	1 cut(s)	1314
ClaI	ATCGAT	1 cut(s)	2307
CseI	GACGC	2 cut(s)	1689, 2534
Csp6I	GTAC	3 cut(s)	420, 817, 2267
CspCI	CAANNNNNGTGG	2 cut(s)	902, 937
CviAII	CATG	9 cut(s)	53, 188, 1002, 1288, 1547, 1874, 2021, 2098, 2116
CviQI	GTAC	3 cut(s)	420, 817, 2267
DdeI	CTNAG	7 cut(s)	35, 167, 587, 938, 1050, 1407, 2505
DpnI	GATC	7 cut(s)	263, 702, 891, 1068, 1752, 2306, 2313
DpnII	GATC	7 cut(s)	261, 700, 889, 1066, 1750, 2304, 2311
DraI	TTTAAA	2 cut(s)	900, 1773
DraIII	CACNNNGTG	2 cut(s)	1492, 1646
DriI	GACNNNNNGTC	3 cut(s)	730, 1375, 2544
EaeI	YGGCCR	2 cut(s)	49, 2210
Eam1104I	CTCTTC	4 cut(s)	874, 2073, 2139, 2212
Eam1105I	GACNNNNNGTC	3 cut(s)	730, 1375, 2544
EarI	CTCTTC	4 cut(s)	874, 2073, 2139, 2212
EciI	GGCGGA	1 cut(s)	984
Ecl136II	GAGCTC	2 cut(s)	663, 774
Eco130I	CCWWGG	3 cut(s)	52, 1453, 1462
Eco24I	GRGCYC	2 cut(s)	665, 776
Eco32I	GATATC	1 cut(s)	306
Eco47I	GGWCC	3 cut(s)	63, 247, 2501
Eco53kI	GAGCTC	2 cut(s)	663, 774
Eco57I	CTGAAG	4 cut(s)	203, 1809, 1959, 2472
Eco72I	CACGTG	1 cut(s)	1686
Eco81I	CCTNAGG	1 cut(s)	2505
Eco88I	CYCGRG	1 cut(s)	1314
Eco91I	GGTNACC	2 cut(s)	1030, 1484
EcoICRI	GAGCTC	2 cut(s)	663, 774
EcoO109I	RGGNCCY	2 cut(s)	247, 2501
EcoO65I	GGTNACC	2 cut(s)	1030, 1484
EcoRI	GAATTC	1 cut(s)	1744
EcoRV	GATATC	1 cut(s)	306
EcoT14I	CCWWGG	3 cut(s)	52, 1453, 1462
EcoT38I	GRGCYC	2 cut(s)	665, 776
ErhI	CCWWGG	3 cut(s)	52, 1453, 1462
Esp3I	CGTCTC	1 cut(s)	339
FaeI	CATG	9 cut(s)	56, 191, 1005, 1291, 1550, 1877, 2024, 2101, 2119
FalI	AAGNNNNNCTT	4 cut(s)	264, 296, 946, 978
FaqI	GGGAC	4 cut(s)	49, 464, 1331, 2514
FatI	CATG	9 cut(s)	52, 187, 1001, 1287, 1546, 1873, 2020, 2097, 2115
FbaI	TGATCA	1 cut(s)	1066
FblI	GTMKAC	1 cut(s)	2438
Fnu4HI	GCNGC	8 cut(s)	40, 1167, 1459, 1812, 1896, 2049, 2150, 2495
FokI	GGATG	5 cut(s)	313, 514, 887, 1508, 1909
FriOI	GRGCYC	2 cut(s)	665, 776
Fsp4HI	GCNGC	8 cut(s)	40, 1167, 1459, 1812, 1896, 2049, 2150, 2495
FspBI	CTAG	2 cut(s)	1721, 1907
GluI	GCNGC	8 cut(s)	40, 1167, 1459, 1812, 1896, 2049, 2150, 2495
GsaI	CCCAGC	1 cut(s)	486
GsuI	CTGGAG	2 cut(s)	363, 1988
HaeIII	GGCC	6 cut(s)	51, 127, 832, 1418, 2212, 2223
HapII	CCGG	4 cut(s)	11, 330, 1315, 1489
HgaI	GACGC	2 cut(s)	1689, 2534
Hin1I	GRCGYC	1 cut(s)	2545
Hin1II	CATG	9 cut(s)	56, 191, 1005, 1291, 1550, 1877, 2024, 2101, 2119
HincII	GTYRAC	3 cut(s)	927, 1353, 2095
HindII	GTYRAC	3 cut(s)	927, 1353, 2095
HindIII	AAGCTT	1 cut(s)	1052
HinfI	GANTC	8 cut(s)	31, 389, 446, 515, 524, 1178, 1865, 2342
HpaII	CCGG	4 cut(s)	11, 330, 1315, 1489
HphI	GGTGA	4 cut(s)	899, 1478, 1860, 2314
Hpy166II	GTNNAC	6 cut(s)	927, 1353, 1562, 2095, 2439, 2549
Hpy8I	GTNNAC	6 cut(s)	927, 1353, 1562, 2095, 2439, 2549
HpyAV	CCTTC	8 cut(s)	499, 602, 865, 1053, 2000, 2242, 2321, 2397
HpyCH4III	ACNGT	4 cut(s)	1030, 1337, 1350, 2436
HpyCH4IV	ACGT	3 cut(s)	1478, 1685, 2416
HpyF10VI	GCNNNNNNNGC	7 cut(s)	48, 57, 169, 1046, 1458, 1901, 1910
HpyF3I	CTNAG	7 cut(s)	35, 167, 587, 938, 1050, 1407, 2505
HpySE526I	ACGT	3 cut(s)	1478, 1685, 2416
Hsp92I	GRCGYC	1 cut(s)	2545
Hsp92II	CATG	9 cut(s)	56, 191, 1005, 1291, 1550, 1877, 2024, 2101, 2119
Ksp22I	TGATCA	1 cut(s)	1066
Kzo9I	GATC	7 cut(s)	261, 700, 889, 1066, 1750, 2304, 2311
LguI	GCTCTTC	1 cut(s)	2139
LmnI	GCTCC	7 cut(s)	57, 160, 779, 1045, 1553, 1936, 2491
Lsp1109I	GCAGC	8 cut(s)	51, 1178, 1445, 1798, 1907, 2060, 2161, 2481
LweI	GCATC	2 cut(s)	214, 1281
MaeI	CTAG	2 cut(s)	1721, 1907
MaeII	ACGT	3 cut(s)	1478, 1685, 2416
MaeIII	GTNAC	5 cut(s)	1030, 1309, 1377, 1484, 1840
MalI	GATC	7 cut(s)	263, 702, 891, 1068, 1752, 2306, 2313
MboI	GATC	7 cut(s)	261, 700, 889, 1066, 1750, 2304, 2311
MhlI	GDGCHC	4 cut(s)	665, 776, 1042, 2115
MlyI	GAGTC	2 cut(s)	1187, 1859
MmeI	TCCRAC	3 cut(s)	362, 366, 1392
MroXI	GAANNNNTTC	3 cut(s)	714, 961, 2252
MspA1I	CMGCKG	1 cut(s)	474
MspI	CCGG	4 cut(s)	11, 330, 1315, 1489
MspR9I	CCNGG	4 cut(s)	11, 1315, 1316, 1490
MvnI	CGCG	1 cut(s)	444
MwoI	GCNNNNNNNGC	7 cut(s)	48, 57, 169, 1046, 1458, 1901, 1910
NciI	CCSGG	4 cut(s)	11, 1315, 1316, 1490
NcoI	CCATGG	1 cut(s)	52
NdeII	GATC	7 cut(s)	261, 700, 889, 1066, 1750, 2304, 2311
NlaIII	CATG	9 cut(s)	56, 191, 1005, 1291, 1550, 1877, 2024, 2101, 2119
NlaIV	GGNNCC	6 cut(s)	65, 1439, 1469, 1595, 1857, 2502
NmeAIII	GCCGAG	1 cut(s)	1924
NmuCI	GTSAC	3 cut(s)	1377, 1484, 1840
NruI	TCGCGA	1 cut(s)	444
PagI	TCATGA	1 cut(s)	1287
PciSI	GCTCTTC	1 cut(s)	2139
PdmI	GAANNNNTTC	3 cut(s)	714, 961, 2252
PfeI	GAWTC	6 cut(s)	31, 389, 446, 515, 524, 2342
PflFI	GACNNNGTC	1 cut(s)	2200
PfoI	TCCNGGA	1 cut(s)	9
PkrI	GCNGC	8 cut(s)	41, 1168, 1460, 1813, 1897, 2050, 2151, 2496
PleI	GAGTC	2 cut(s)	1186, 1859
PmaCI	CACGTG	1 cut(s)	1686
PmlI	CACGTG	1 cut(s)	1686
PpsI	GAGTC	2 cut(s)	1186, 1859
Ppu21I	YACGTR	1 cut(s)	1686
PpuMI	RGGWCCY	2 cut(s)	247, 2501
Psp124BI	GAGCTC	2 cut(s)	665, 776
Psp5II	RGGWCCY	2 cut(s)	247, 2501
PspCI	CACGTG	1 cut(s)	1686
PspEI	GGTNACC	2 cut(s)	1030, 1484
PspFI	CCCAGC	1 cut(s)	482
PspN4I	GGNNCC	6 cut(s)	65, 1439, 1469, 1595, 1857, 2502
PspPI	GGNCC	3 cut(s)	63, 247, 2501
PspPPI	RGGWCCY	2 cut(s)	247, 2501
PstI	CTGCAG	1 cut(s)	2499
PsyI	GACNNNGTC	1 cut(s)	2200
PvuII	CAGCTG	1 cut(s)	474
RruI	TCGCGA	1 cut(s)	444
RsaI	GTAC	3 cut(s)	421, 818, 2268
RsaNI	GTAC	3 cut(s)	420, 817, 2267
SacI	GAGCTC	2 cut(s)	665, 776
SapI	GCTCTTC	1 cut(s)	2139
SatI	GCNGC	8 cut(s)	40, 1167, 1459, 1812, 1896, 2049, 2150, 2495
Sau3AI	GATC	7 cut(s)	261, 700, 889, 1066, 1750, 2304, 2311
Sau96I	GGNCC	3 cut(s)	63, 247, 2501
SchI	GAGTC	2 cut(s)	1187, 1859
ScrFI	CCNGG	4 cut(s)	11, 1315, 1316, 1490
SduI	GDGCHC	4 cut(s)	665, 776, 1042, 2115
SfaNI	GCATC	2 cut(s)	214, 1281
SfcI	CTRYAG	1 cut(s)	2495
SinI	GGWCC	3 cut(s)	63, 247, 2501
SmaI	CCCGGG	1 cut(s)	1316
SmiI	ATTTAAAT	1 cut(s)	1773
SmlI	CTYRAG	1 cut(s)	1213
SmoI	CTYRAG	1 cut(s)	1213
SsiI	CCGC	3 cut(s)	969, 1402, 1710
SspI	AATATT	1 cut(s)	17
SspMI	CTAG	2 cut(s)	1721, 1907
SstI	GAGCTC	2 cut(s)	665, 776
StyD4I	CCNGG	4 cut(s)	9, 1313, 1314, 1488
StyI	CCWWGG	3 cut(s)	52, 1453, 1462
SwaI	ATTTAAAT	1 cut(s)	1773
TaaI	ACNGT	4 cut(s)	1030, 1337, 1350, 2436
TaiI	ACGT	3 cut(s)	1481, 1688, 2419
TaqI	TCGA	6 cut(s)	416, 544, 659, 2204, 2228, 2307
TatI	WGTACW	1 cut(s)	2266
TfiI	GAWTC	6 cut(s)	31, 389, 446, 515, 524, 2342
TscAI	CASTG	3 cut(s)	595, 1309, 1641
TseFI	GTSAC	3 cut(s)	1377, 1484, 1840
TseI	GCWGC	8 cut(s)	39, 1166, 1458, 1811, 1895, 2048, 2149, 2494
Tsp45I	GTSAC	3 cut(s)	1377, 1484, 1840
TspDTI	ATGAA	9 cut(s)	231, 269, 518, 594, 609, 723, 921, 1128, 2091
TspGWI	ACGGA	3 cut(s)	162, 1094, 1240
TspMI	CCCGGG	1 cut(s)	1314
TspRI	CASTG	3 cut(s)	595, 1309, 1641
Tth111I	GACNNNGTC	1 cut(s)	2200
VpaK11BI	GGWCC	3 cut(s)	63, 247, 2501
XapI	RAATTY	6 cut(s)	845, 1744, 2125, 2170, 2380, 2515
XbaI	TCTAGA	1 cut(s)	1720
XmaI	CCCGGG	1 cut(s)	1314
XmiI	GTMKAC	1 cut(s)	2438
XmnI	GAANNNNTTC	3 cut(s)	714, 961, 2252
XspI	CTAG	2 cut(s)	1721, 1907

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.