Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr17

Orthogroup ID

OG0003069

Physical Location & Seq

Genomic Coordinates Forward (+)

3641289 .. 3644535

Gene Structure 3247 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom17g05270.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2655 bp

ATGTATTTGCCGGGATATTGTTCCATACAGAATCTAAGCAGCAATGTCGGCCATGGGAGCTGGTCCCTACTCCACGAGAACAGAAACTTGAACAATGGGCAACAATACGAAGTGTTCTTGACAAGGCCAGTTATTGATGGATTTCACGAACATGATAAGGAGCAACTGAGGCAAACTATTCTGAAGCATGAGACAATTTTCAGGCATCAGCTCAATGAACTCCATCGTCTCTACGAAAGGCAAAAGGACCTGATGAACGAGATCAAAAGCAAAGAGCTTCTCAAACATAAAAAAGTAGCAGGGATATCACAATCAACTTTCTCATCATCCAGCTTTCCGACTGGAGACGACAGAAAGAGTTGGCATATTTCTAATTTACCCTTGGTGGATTCCAGTTATGGTAGACAATCTACATTGAGTACTTGTATCAGCCAATCTCCTTGTGATTCTATTGGAAAAACCCTGCGAAACAGCAGTCTCCCCAGCCAGAGTAGAGTAGGGATGAAGGACCATGAATCTTTGGATTCCAGAGTGAAGAAGCCTCGAAGAAGATTGTTCAATCTTGAACTTCCAGCCGATGAATACCTCAGTGATGAAGAAGAACCAGAACGAGGTTTGGGGTCGGGAACTCACAAATCTGGCTATAACAATAGTGTGTCGAGCTCTGGTTTGCATTTAGTAAGAAGCATTGGTTTGGCTGATCGGAATGAACCTATTCAGATAGACAAAGTGTCTGCTTCAACTTCTGTCATAATGTCTGGCAACCAACCGAGCTCCAAAGAGGAGATAGAAAGGCAGGTTTTATCAGCAAATGCGTACAAAGTTATGTGGCCTTTTGCAAAGAAATTCCCTGAAAACCCACAAACGGGGAAGGATGGAAGAGTTGGTGATCTGCATTTAAAGAATGAAATCCAAAAAGAGTGGTCAACAAATGCTCTCAGAGATGAGCAAACAAGAAATTCTTCTGGCGGAAAGTTTGGTCTTCAAGATTTTAATAAGCCATGTGAATCATCAGAAAACGAAGCTAGGATACCCTGTGATCGTCCTGCGAAGGTTTTCACTTTTGATCAGAATAAAACGGAGAAACGAATAAAGAGGACACTTTTTGGTATAGAAATCTCTGAAAGCGATATAAGTTCATCTGCTATGACTTCCCAATCTGATGCAGCCATTTCCGAGTCATCTCCTAATTGGACAATACCTCCAAGTAGCTTAAGCCAGAACTTAACATCGATTCAAGTAAGCACTTCCATTAATACCTCCACACAATCTAATAGCGCTTCACTCATGATGCCACAAAGCCGTGAAGTTACCAAGGACAGATTGCTTCTAGACTGTAATTCAATACCGTCAACTCCCAGTCTGAAAGACGAAGTGTCACACCAAAGCGGTTTTTTCTTCCGCGCTAAGTTGGAGGCCAATGAATTACAGGCTTGTCACCCCTCAATGAGCCTTGCCTGCCAAGGGAACCATTCAGCGTCAGGTCACCAGGTGGATGTCAAACCTGCTATTGATGTGAATGATGTTCCTTCTAATAAATATCTGCATGAAGGAGCTTGTGAACAAAATATAATTTCTCTGAATGGGCTGAGGAACCAAGAAAGCCGACAAGGAAGATTGCATTGGCTTGAAGCAGTGCCACTTTGTGATGGCGAATCTTCCAAAGAAATTGAAAGCAGACGCATATGTAGAGAGCAACTGAAAGCAGACAGTCTGATTCTAGACAAGGGATTTTCAAATGAGAATTCTGGTTCAAGACACCAAATAGATTTAAATAGATGTTTAACCGAAGAAGAAACTGAAACGACTGCCGCTTCCTCAATTATGAGGACAGAAACTGTGATAGATTTGGAGGCACCGGTGGTAGTTGAAACAAATATATATGGGGAAGATTCCACTGAAAGAAGACGTAAGGAACTTCTTGACTCACCTCATGAGGGCCTTATCAGAGTTGCAGCCGAGGCTCTAGTTGCCATCTCAGCATCTCAAGCTCCCAAGATGCAGGAAAATACCACTTGTTGCAATCTGGAAGCTTCGGAAAACGACTCCCTTCTTTGGTTTGCTGAAGTAATTTCTTCACATGATGGAAATCTTGACAACGGAAATGCGGCAGAGGTTAAGCCGACTTCCTGTGATGAAGAGGCTTTTCCCGTTGACATGGATTATTTTGAGCACATGACATTAAATTTGGTGGAAACCAAAGAAGAGCAGCCATGCTATGTGCCTCCAAATTCAGACAACCTCATAGAGGAAGGGACATTGTCAAAACGGCCACAAAGAGGCAAATCGAGGAGGGGGAGGCAAAGGAAGGACTTCGGGAGAGATGTACTTCCCGGTCTTGCTTCTTTGTCACAAAATGAGGTGACTGATGATCTTCAAATAATTGAAGGGTTGATTAGGGAATCTGGTGGGAGATGGCAGTCAAGTTTGTCACATAGAAATTCCGGTAAGGGTGGTAGGGGAAGGGGGAGGAAGCGTATGGGAACTACTGCTCCATCTACTAGTGTGGCTGAAGTTAGCCGGCCCCAGATTGAGCAACCAAAATGCGAGCTGCAGGGACCTGAGGAGAGAAACTTCACCTTTCGGGGTAAGAGGACAAGGCGCCCACCACGGCAAAGATATCCCATTGATAATTCTCCTGTTCCTCAAACCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF863

Protein Analysis

885

Amino Acids

98.79

Weight (kDa)

6.06

Isoelectric Point (pI)

62.86

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003069)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g47740 FvH4_6g47740 FvH4_6g47740 FvH4_6g47740
malus_domestica	MD09G1059300.v1.1 MD17G1054900.v1.1
prunus_persica	Prupe.3G261200_v2.0.a1
pyrus_communis	pycom08g18570 pycom111g05020 pycom111g05040 pycom111g05060 pycom17g05270 pycom17g05290
rosa_chinensis	RchiOBHm_Chr2g0166861 RchiOBHm_Chr4g0401141 RchiOBHm_Chr5g0037041 RchiOBHm_Chr7g0237831
rosa_laevigata	RLG00000021662 RLG00000021675
rosa_multiflora	Rmu_sc0005344.1_g000006
rosa_roxburghii	Rroxscaffold_2G00084060 Rroxscaffold_2G00084090 Rroxscaffold_2G00084470 Rroxscaffold_4G00327630
rosa_rugosa	Rorug02G0525900 Rorug02G0526000
rosa_samantha	Rh2AG592900 Rh2BG604100 Rh2BG604300 Rh2CG575000 Rh2CG575200 Rh2DG615600 Rh2DG615800 Rh3DG312900 Rh4DG186200 Rh5DG059300 Rh5DG383900
rosa_wichuraiana	Rw2G049350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	787, 1513
AccB1I	GGYRCC	2 cut(s)	1855, 2601
AccI	GTMKAC	1 cut(s)	403
AccII	CGCG	1 cut(s)	1404
AciI	CCGC	5 cut(s)	969, 1389, 1402, 1812, 2108
AcoI	YGGCCR	2 cut(s)	49, 2270
AcsI	RAATTY	6 cut(s)	845, 958, 1744, 2185, 2230, 2440
AcuI	CTGAAG	3 cut(s)	203, 2085, 2532
AcyI	GRCGYC	1 cut(s)	2602
AdeI	CACNNNGTG	2 cut(s)	1492, 1646
AfaI	GTAC	3 cut(s)	421, 818, 2328
AfeI	AGCGCT	1 cut(s)	1279
AfiI	CCNNNNNNNGG	8 cut(s)	611, 865, 866, 1464, 1937, 2055, 2248, 2279
AflII	CTTAAG	1 cut(s)	1213
AgeI	ACCGGT	1 cut(s)	1858
AhdI	GACNNNNNGTC	2 cut(s)	730, 1375
AhlI	ACTAGT	1 cut(s)	2501
AjnI	CCWGG	1 cut(s)	1488
AjuI	GAANNNNNNNTTGG	2 cut(s)	1606, 1638
AloI	GAACNNNNNNTCC	2 cut(s)	2476, 2508
Alw21I	GWGCWC	3 cut(s)	665, 776, 2175
Alw26I	GTCTC	4 cut(s)	185, 233, 339, 482
AlwNI	CAGNNNCTG	1 cut(s)	1838
Aor51HI	AGCGCT	1 cut(s)	1279
AoxI	GGCC	7 cut(s)	49, 125, 830, 1416, 1939, 2270, 2522
ApeKI	GCWGC	5 cut(s)	39, 1166, 1955, 2209, 2551
ApoI	RAATTY	6 cut(s)	845, 958, 1744, 2185, 2230, 2440
ArsI	GACNNNNNNTTYG	4 cut(s)	460, 492, 2170, 2202
AseI	ATTAAT	1 cut(s)	1254
AsiGI	ACCGGT	1 cut(s)	1858
Asp700I	GAANNNNTTC	4 cut(s)	714, 961, 1055, 2312
AspLEI	GCGC	3 cut(s)	1280, 1406, 2604
AspS9I	GGNCC	6 cut(s)	63, 247, 508, 1939, 2523, 2558
AsuC2I	CCSGG	2 cut(s)	12, 2334
AsuHPI	GGTGA	6 cut(s)	899, 1430, 1478, 1920, 2374, 2569
AvaII	GGWCC	4 cut(s)	63, 247, 508, 2558
AxyI	CCTNAGG	1 cut(s)	2562
BanI	GGYRCC	2 cut(s)	1855, 2601
BanII	GRGCYC	2 cut(s)	665, 776
BarI	GAAGNNNNNNTAC	2 cut(s)	1014, 1046
BauI	CACGAG	1 cut(s)	74
BbsI	GAAGAC	2 cut(s)	974, 1912
Bbv12I	GWGCWC	3 cut(s)	665, 776, 2175
BbvCI	CCTCAGC	1 cut(s)	1589
BbvI	GCAGC	5 cut(s)	51, 1178, 1967, 2221, 2538
BccI	CCATC	8 cut(s)	131, 231, 869, 1643, 1982, 2078, 2409, 2503
BceAI	ACGGC	3 cut(s)	1287, 2285, 2627
BcgI	CGANNNNNNTGC	2 cut(s)	28, 62
BciT130I	CCWGG	1 cut(s)	1490
BciVI	GTATCC	1 cut(s)	1023
BclI	TGATCA	1 cut(s)	1066
BcnI	CCSGG	2 cut(s)	12, 2334
BcoDI	GTCTC	4 cut(s)	185, 233, 339, 482
BcuI	ACTAGT	1 cut(s)	2501
BfaI	CTAG	5 cut(s)	1026, 1331, 1721, 1967, 2502
BfmI	CTRYAG	1 cut(s)	2552
BfoI	RGCGCY	2 cut(s)	1281, 2605
BfrI	CTTAAG	1 cut(s)	1213
BfuAI	ACCTGC	2 cut(s)	787, 1513
BfuI	GTATCC	1 cut(s)	1023
BisI	GCNGC	7 cut(s)	40, 1167, 1812, 1956, 2109, 2210, 2552
BlsI	GCNGC	7 cut(s)	41, 1168, 1813, 1957, 2110, 2211, 2553
BmcAI	AGTACT	1 cut(s)	421
Bme1390I	CCNGG	3 cut(s)	12, 1490, 2334
Bme18I	GGWCC	4 cut(s)	63, 247, 508, 2558
BmeRI	GACNNNNNGTC	2 cut(s)	730, 1375
BmgT120I	GGNCC	6 cut(s)	63, 247, 508, 1939, 2523, 2558
BmiI	GGNNCC	7 cut(s)	65, 1469, 1595, 1857, 2525, 2559, 2603
BmrFI	CCNGG	3 cut(s)	12, 1490, 2334
BmrI	ACTGGG	1 cut(s)	1353
BmsI	GCATC	5 cut(s)	214, 1153, 1281, 1989, 1991
BmuI	ACTGGG	1 cut(s)	1353
BpiI	GAAGAC	2 cut(s)	974, 1912
BpmI	CTGGAG	1 cut(s)	363
Bpu10I	CCTNAGC	1 cut(s)	1589
BpuEI	CTTGAG	1 cut(s)	1971
BpuMI	CCSGG	2 cut(s)	12, 2334
Bsa29I	ATCGAT	1 cut(s)	1232
BsaBI	GATNNNNATC	1 cut(s)	2088
BsaHI	GRCGYC	1 cut(s)	2602
BsaJI	CCNNGG	6 cut(s)	52, 381, 1314, 1462, 1959, 2609
BsaWI	WCCGGW	2 cut(s)	1858, 2444
BsaXI	ACNNNNNCTCC	6 cut(s)	1186, 1216, 2461, 2476, 2491, 2506
Bsc4I	CCNNNNNNNGG	8 cut(s)	611, 865, 866, 1464, 1937, 2055, 2248, 2279
Bse118I	RCCGGY	2 cut(s)	1858, 2520
Bse1I	ACTGG	4 cut(s)	128, 346, 393, 1359
Bse21I	CCTNAGG	1 cut(s)	2562
Bse3DI	GCAATG	1 cut(s)	49
Bse8I	GATNNNNATC	1 cut(s)	2088
BseBI	CCWGG	1 cut(s)	1490
BseCI	ATCGAT	1 cut(s)	1232
BseDI	CCNNGG	6 cut(s)	52, 381, 1314, 1462, 1959, 2609
BseGI	GGATG	4 cut(s)	326, 507, 880, 1501
BseJI	GATNNNNATC	1 cut(s)	2088
BseLI	CCNNNNNNNGG	8 cut(s)	611, 865, 866, 1464, 1937, 2055, 2248, 2279
BseMI	GCAATG	1 cut(s)	49
BseMII	CTCAG	6 cut(s)	158, 601, 952, 1580, 1992, 2553
BseNI	ACTGG	4 cut(s)	128, 346, 393, 1359
BseRI	GAGGAG	3 cut(s)	797, 2305, 2579
BseXI	GCAGC	5 cut(s)	51, 1178, 1967, 2221, 2538
BseYI	CCCAGC	1 cut(s)	482
Bsh1236I	CGCG	1 cut(s)	1404
BshFI	GGCC	7 cut(s)	51, 127, 832, 1418, 1941, 2272, 2524
BshNI	GGYRCC	2 cut(s)	1855, 2601
BshTI	ACCGGT	1 cut(s)	1858
BshVI	ATCGAT	1 cut(s)	1232
BsiHKAI	GWGCWC	3 cut(s)	665, 776, 2175
BsiSI	CCGG	5 cut(s)	11, 1859, 2334, 2445, 2521
BslFI	GGGAC	3 cut(s)	49, 2269, 2571
BslI	CCNNNNNNNGG	8 cut(s)	611, 865, 866, 1464, 1937, 2055, 2248, 2279
BsmAI	GTCTC	4 cut(s)	185, 233, 339, 482
BsmBI	CGTCTC	2 cut(s)	233, 339
BsmFI	GGGAC	3 cut(s)	49, 2269, 2571
BsnI	GGCC	7 cut(s)	51, 127, 832, 1418, 1941, 2272, 2524
Bsp1286I	GDGCHC	3 cut(s)	665, 776, 2175
Bsp143I	GATC	6 cut(s)	261, 700, 889, 1039, 1066, 2371
Bsp19I	CCATGG	1 cut(s)	52
BspACI	CCGC	5 cut(s)	969, 1389, 1402, 1812, 2108
BspANI	GGCC	7 cut(s)	51, 127, 832, 1418, 1941, 2272, 2524
BspCNI	CTCAG	6 cut(s)	159, 600, 951, 1581, 1991, 2554
BspDI	ATCGAT	1 cut(s)	1232
BspFNI	CGCG	1 cut(s)	1404
BspHI	TCATGA	2 cut(s)	1287, 1933
BspLI	GGNNCC	7 cut(s)	65, 1469, 1595, 1857, 2525, 2559, 2603
BspMAI	CTGCAG	1 cut(s)	2556
BspMI	ACCTGC	2 cut(s)	787, 1513
BspQI	GCTCTTC	1 cut(s)	2199
BspT107I	GGYRCC	2 cut(s)	1855, 2601
BspTI	CTTAAG	1 cut(s)	1213
BsrDI	GCAATG	1 cut(s)	49
BsrFI	RCCGGY	2 cut(s)	1858, 2520
BsrI	ACTGG	4 cut(s)	128, 346, 393, 1359
BssAI	RCCGGY	2 cut(s)	1858, 2520
BssECI	CCNNGG	6 cut(s)	52, 381, 1314, 1462, 1959, 2609
BssMI	GATC	6 cut(s)	261, 700, 889, 1039, 1066, 2371
BssNI	GRCGYC	1 cut(s)	2602
BssSI	CACGAG	1 cut(s)	74
BssT1I	CCWWGG	4 cut(s)	52, 381, 1314, 1462
Bst2BI	CACGAG	1 cut(s)	74
Bst2UI	CCWGG	1 cut(s)	1490
Bst4CI	ACNGT	4 cut(s)	1337, 1350, 1712, 1840
Bst6I	CTCTTC	3 cut(s)	874, 2133, 2199
BstACI	GRCGYC	1 cut(s)	2602
BstAFI	CTTAAG	1 cut(s)	1213
BstC8I	GCNNGC	3 cut(s)	1459, 2522, 2549
BstDEI	CTNAG	8 cut(s)	35, 167, 587, 938, 1407, 1589, 1978, 2562
BstDSI	CCRYGG	2 cut(s)	52, 2609
BstEII	GGTNACC	1 cut(s)	1484
BstENI	CCTNNNNNAGG	1 cut(s)	2246
BstF5I	GGATG	4 cut(s)	326, 507, 880, 1501
BstFNI	CGCG	1 cut(s)	1404
BstH2I	RGCGCY	2 cut(s)	1281, 2605
BstHHI	GCGC	3 cut(s)	1280, 1406, 2604
BstKTI	GATC	6 cut(s)	264, 703, 892, 1042, 1069, 2374
BstMAI	GTCTC	4 cut(s)	185, 233, 339, 482
BstMBI	GATC	6 cut(s)	261, 700, 889, 1039, 1066, 2371
BstMWI	GCNNNNNNNGC	8 cut(s)	48, 57, 169, 1458, 1961, 1970, 1979, 1988
BstNI	CCWGG	1 cut(s)	1490
BstPI	GGTNACC	1 cut(s)	1484
BstSCI	CCNGG	3 cut(s)	10, 1488, 2332
BstSFI	CTRYAG	1 cut(s)	2552
BstUI	CGCG	1 cut(s)	1404
BstV1I	GCAGC	5 cut(s)	51, 1178, 1967, 2221, 2538
BstV2I	GAAGAC	2 cut(s)	974, 1912
Bsu15I	ATCGAT	1 cut(s)	1232
Bsu36I	CCTNAGG	1 cut(s)	2562
BsuI	GTATCC	1 cut(s)	1023
BsuRI	GGCC	7 cut(s)	51, 127, 832, 1418, 1941, 2272, 2524
BsuTUI	ATCGAT	1 cut(s)	1232
BtgI	CCRYGG	2 cut(s)	52, 2609
BtsCI	GGATG	4 cut(s)	326, 507, 880, 1501
BtsI	GCAGTG	1 cut(s)	1641
BtsIMutI	CAGTG	3 cut(s)	595, 1641, 1896
BveI	ACCTGC	2 cut(s)	787, 1513
Cac8I	GCNNGC	3 cut(s)	1459, 2522, 2549
CaiI	CAGNNNCTG	1 cut(s)	1838
CciI	TCATGA	2 cut(s)	1287, 1933
CfoI	GCGC	3 cut(s)	1280, 1406, 2604
Cfr10I	RCCGGY	2 cut(s)	1858, 2520
Cfr13I	GGNCC	6 cut(s)	63, 247, 508, 1939, 2523, 2558
ClaI	ATCGAT	1 cut(s)	1232
CseI	GACGC	2 cut(s)	1467, 1689
CsiI	ACCWGGT	1 cut(s)	1488
Csp6I	GTAC	3 cut(s)	420, 817, 2327
CspAI	ACCGGT	1 cut(s)	1858
CspCI	CAANNNNNGTGG	2 cut(s)	902, 937
CviQI	GTAC	3 cut(s)	420, 817, 2327
DdeI	CTNAG	8 cut(s)	35, 167, 587, 938, 1407, 1589, 1978, 2562
DinI	GGCGCC	1 cut(s)	2603
DpnI	GATC	6 cut(s)	263, 702, 891, 1041, 1068, 2373
DpnII	GATC	6 cut(s)	261, 700, 889, 1039, 1066, 2371
DraI	TTTAAA	2 cut(s)	900, 1773
DraIII	CACNNNGTG	2 cut(s)	1492, 1646
DriI	GACNNNNNGTC	2 cut(s)	730, 1375
EaeI	YGGCCR	2 cut(s)	49, 2270
Eam1104I	CTCTTC	3 cut(s)	874, 2133, 2199
Eam1105I	GACNNNNNGTC	2 cut(s)	730, 1375
EarI	CTCTTC	3 cut(s)	874, 2133, 2199
EciI	GGCGGA	1 cut(s)	984
Ecl136II	GAGCTC	2 cut(s)	663, 774
Eco130I	CCWWGG	4 cut(s)	52, 381, 1314, 1462
Eco24I	GRGCYC	2 cut(s)	665, 776
Eco32I	GATATC	2 cut(s)	306, 2621
Eco47I	GGWCC	4 cut(s)	63, 247, 508, 2558
Eco47III	AGCGCT	1 cut(s)	1279
Eco53kI	GAGCTC	2 cut(s)	663, 774
Eco57I	CTGAAG	3 cut(s)	203, 2085, 2532
Eco81I	CCTNAGG	1 cut(s)	2562
Eco91I	GGTNACC	1 cut(s)	1484
EcoICRI	GAGCTC	2 cut(s)	663, 774
EcoNI	CCTNNNNNAGG	1 cut(s)	2246
EcoO109I	RGGNCCY	3 cut(s)	247, 1939, 2558
EcoO65I	GGTNACC	1 cut(s)	1484
EcoRI	GAATTC	1 cut(s)	1744
EcoRII	CCWGG	1 cut(s)	1488
EcoRV	GATATC	2 cut(s)	306, 2621
EcoT14I	CCWWGG	4 cut(s)	52, 381, 1314, 1462
EcoT38I	GRGCYC	2 cut(s)	665, 776
EgeI	GGCGCC	1 cut(s)	2603
EheI	GGCGCC	1 cut(s)	2603
ErhI	CCWWGG	4 cut(s)	52, 381, 1314, 1462
Esp3I	CGTCTC	2 cut(s)	233, 339
FalI	AAGNNNNNCTT	2 cut(s)	946, 978
FaqI	GGGAC	3 cut(s)	49, 2269, 2571
FauNDI	CATATG	1 cut(s)	1685
FbaI	TGATCA	1 cut(s)	1066
FblI	GTMKAC	1 cut(s)	403
Fnu4HI	GCNGC	7 cut(s)	40, 1167, 1812, 1956, 2109, 2210, 2552
FokI	GGATG	4 cut(s)	313, 514, 887, 1508
FriOI	GRGCYC	2 cut(s)	665, 776
Fsp4HI	GCNGC	7 cut(s)	40, 1167, 1812, 1956, 2109, 2210, 2552
FspBI	CTAG	5 cut(s)	1026, 1331, 1721, 1967, 2502
GlaI	GCGC	3 cut(s)	1279, 1405, 2603
GluI	GCNGC	7 cut(s)	40, 1167, 1812, 1956, 2109, 2210, 2552
GsaI	CCCAGC	1 cut(s)	486
GsuI	CTGGAG	1 cut(s)	363
HaeII	RGCGCY	2 cut(s)	1281, 2605
HaeIII	GGCC	7 cut(s)	51, 127, 832, 1418, 1941, 2272, 2524
HapII	CCGG	5 cut(s)	11, 1859, 2334, 2445, 2521
HgaI	GACGC	2 cut(s)	1467, 1689
HhaI	GCGC	3 cut(s)	1280, 1406, 2604
Hin1I	GRCGYC	1 cut(s)	2602
Hin6I	GCGC	3 cut(s)	1278, 1404, 2602
HinP1I	GCGC	3 cut(s)	1278, 1404, 2602
HincII	GTYRAC	3 cut(s)	927, 1353, 2155
HindII	GTYRAC	3 cut(s)	927, 1353, 2155
HindIII	AAGCTT	1 cut(s)	2031
HpaII	CCGG	5 cut(s)	11, 1859, 2334, 2445, 2521
HphI	GGTGA	6 cut(s)	899, 1430, 1478, 1920, 2374, 2569
Hpy166II	GTNNAC	5 cut(s)	404, 927, 1353, 1562, 2155
Hpy8I	GTNNAC	5 cut(s)	404, 927, 1353, 1562, 2155
HpyCH4III	ACNGT	4 cut(s)	1337, 1350, 1712, 1840
HpyCH4IV	ACGT	1 cut(s)	1909
HpyF10VI	GCNNNNNNNGC	8 cut(s)	48, 57, 169, 1458, 1961, 1970, 1979, 1988
HpyF3I	CTNAG	8 cut(s)	35, 167, 587, 938, 1407, 1589, 1978, 2562
HpySE526I	ACGT	1 cut(s)	1909
Hsp92I	GRCGYC	1 cut(s)	2602
HspAI	GCGC	3 cut(s)	1278, 1404, 2602
KasI	GGCGCC	1 cut(s)	2601
KroI	GCCGGC	1 cut(s)	2520
KroNI	GCCGGC	1 cut(s)	2522
Ksp22I	TGATCA	1 cut(s)	1066
Kzo9I	GATC	6 cut(s)	261, 700, 889, 1039, 1066, 2371
LguI	GCTCTTC	1 cut(s)	2199
LmnI	GCTCC	6 cut(s)	57, 160, 779, 1553, 1996, 2497
Lsp1109I	GCAGC	5 cut(s)	51, 1178, 1967, 2221, 2538
LweI	GCATC	5 cut(s)	214, 1153, 1281, 1989, 1991
MabI	ACCWGGT	1 cut(s)	1488
MaeI	CTAG	5 cut(s)	1026, 1331, 1721, 1967, 2502
MaeII	ACGT	1 cut(s)	1909
MaeIII	GTNAC	7 cut(s)	1309, 1377, 1436, 1484, 2349, 2362, 2430
MalI	GATC	6 cut(s)	263, 702, 891, 1041, 1068, 2373
MboI	GATC	6 cut(s)	261, 700, 889, 1039, 1066, 2371
MhlI	GDGCHC	3 cut(s)	665, 776, 2175
Mly113I	GGCGCC	1 cut(s)	2602
MlyI	GAGTC	3 cut(s)	1187, 1919, 2039
MmeI	TCCRAC	2 cut(s)	362, 1392
MroNI	GCCGGC	1 cut(s)	2520
MroXI	GAANNNNTTC	4 cut(s)	714, 961, 1055, 2312
MseI	TTAA	9 cut(s)	899, 993, 1214, 1226, 1254, 1772, 1784, 2118, 2183
MslI	CAYNNNNRTG	1 cut(s)	150
MspCI	CTTAAG	1 cut(s)	1213
MspI	CCGG	5 cut(s)	11, 1859, 2334, 2445, 2521
MspR9I	CCNGG	3 cut(s)	12, 1490, 2334
MvaI	CCWGG	1 cut(s)	1490
MvnI	CGCG	1 cut(s)	1404
MwoI	GCNNNNNNNGC	8 cut(s)	48, 57, 169, 1458, 1961, 1970, 1979, 1988
NaeI	GCCGGC	1 cut(s)	2522
NarI	GGCGCC	1 cut(s)	2602
NciI	CCSGG	2 cut(s)	12, 2334
NcoI	CCATGG	1 cut(s)	52
NdeI	CATATG	1 cut(s)	1685
NdeII	GATC	6 cut(s)	261, 700, 889, 1039, 1066, 2371
NgoMIV	GCCGGC	1 cut(s)	2520
NlaIV	GGNNCC	7 cut(s)	65, 1469, 1595, 1857, 2525, 2559, 2603
NmeAIII	GCCGAG	1 cut(s)	1984
NmuCI	GTSAC	6 cut(s)	1377, 1436, 1484, 2349, 2362, 2430
PagI	TCATGA	2 cut(s)	1287, 1933
PciSI	GCTCTTC	1 cut(s)	2199
PdiI	GCCGGC	1 cut(s)	2522
PdmI	GAANNNNTTC	4 cut(s)	714, 961, 1055, 2312
PflFI	GACNNNGTC	1 cut(s)	2260
PinAI	ACCGGT	1 cut(s)	1858
PkrI	GCNGC	7 cut(s)	41, 1168, 1813, 1957, 2110, 2211, 2553
PleI	GAGTC	3 cut(s)	1186, 1919, 2039
PluTI	GGCGCC	1 cut(s)	2605
PpsI	GAGTC	3 cut(s)	1186, 1919, 2039
PpuMI	RGGWCCY	2 cut(s)	247, 2558
PshBI	ATTAAT	1 cut(s)	1254
Psp124BI	GAGCTC	2 cut(s)	665, 776
Psp5II	RGGWCCY	2 cut(s)	247, 2558
Psp6I	CCWGG	1 cut(s)	1488
PspEI	GGTNACC	1 cut(s)	1484
PspFI	CCCAGC	1 cut(s)	482
PspGI	CCWGG	1 cut(s)	1488
PspN4I	GGNNCC	7 cut(s)	65, 1469, 1595, 1857, 2525, 2559, 2603
PspPI	GGNCC	6 cut(s)	63, 247, 508, 1939, 2523, 2558
PspPPI	RGGWCCY	2 cut(s)	247, 2558
PstI	CTGCAG	1 cut(s)	2556
PstNI	CAGNNNCTG	1 cut(s)	1838
PsyI	GACNNNGTC	1 cut(s)	2260
RsaI	GTAC	3 cut(s)	421, 818, 2328
RsaNI	GTAC	3 cut(s)	420, 817, 2327
RseI	CAYNNNNRTG	1 cut(s)	150
SacI	GAGCTC	2 cut(s)	665, 776
SapI	GCTCTTC	1 cut(s)	2199
SaqAI	TTAA	9 cut(s)	899, 993, 1214, 1226, 1254, 1772, 1784, 2118, 2183
SatI	GCNGC	7 cut(s)	40, 1167, 1812, 1956, 2109, 2210, 2552
Sau3AI	GATC	6 cut(s)	261, 700, 889, 1039, 1066, 2371
Sau96I	GGNCC	6 cut(s)	63, 247, 508, 1939, 2523, 2558
ScaI	AGTACT	1 cut(s)	421
SchI	GAGTC	3 cut(s)	1187, 1919, 2039
ScrFI	CCNGG	3 cut(s)	12, 1490, 2334
SduI	GDGCHC	3 cut(s)	665, 776, 2175
SexAI	ACCWGGT	1 cut(s)	1488
SfaNI	GCATC	5 cut(s)	214, 1153, 1281, 1989, 1991
SfcI	CTRYAG	1 cut(s)	2552
SfoI	GGCGCC	1 cut(s)	2603
SgrAI	CRCCGGYG	1 cut(s)	1858
SinI	GGWCC	4 cut(s)	63, 247, 508, 2558
SmiI	ATTTAAAT	1 cut(s)	1773
SmiMI	CAYNNNNRTG	1 cut(s)	150
SmlI	CTYRAG	2 cut(s)	1213, 1986
SmoI	CTYRAG	2 cut(s)	1213, 1986
SpeI	ACTAGT	1 cut(s)	2501
SsiI	CCGC	5 cut(s)	969, 1389, 1402, 1812, 2108
SspDI	GGCGCC	1 cut(s)	2601
SspMI	CTAG	5 cut(s)	1026, 1331, 1721, 1967, 2502
SstI	GAGCTC	2 cut(s)	665, 776
StyD4I	CCNGG	3 cut(s)	10, 1488, 2332
StyI	CCWWGG	4 cut(s)	52, 381, 1314, 1462
SwaI	ATTTAAAT	1 cut(s)	1773
TaaI	ACNGT	4 cut(s)	1337, 1350, 1712, 1840
TaiI	ACGT	1 cut(s)	1912
TaqI	TCGA	4 cut(s)	544, 659, 1232, 2288
TatI	WGTACW	2 cut(s)	419, 2326
TauI	GCSGC	2 cut(s)	1814, 2111
Tru1I	TTAA	9 cut(s)	899, 993, 1214, 1226, 1254, 1772, 1784, 2118, 2183
Tru9I	TTAA	9 cut(s)	899, 993, 1214, 1226, 1254, 1772, 1784, 2118, 2183
TscAI	CASTG	3 cut(s)	595, 1641, 1903
TseFI	GTSAC	6 cut(s)	1377, 1436, 1484, 2349, 2362, 2430
TseI	GCWGC	5 cut(s)	39, 1166, 1955, 2209, 2551
Tsp45I	GTSAC	6 cut(s)	1377, 1436, 1484, 2349, 2362, 2430
TspGWI	ACGGA	2 cut(s)	1094, 2115
TspRI	CASTG	3 cut(s)	595, 1641, 1903
Tth111I	GACNNNGTC	1 cut(s)	2260
Vha464I	CTTAAG	1 cut(s)	1213
VpaK11BI	GGWCC	4 cut(s)	63, 247, 508, 2558
VspI	ATTAAT	1 cut(s)	1254
XagI	CCTNNNNNAGG	1 cut(s)	2246
XapI	RAATTY	6 cut(s)	845, 958, 1744, 2185, 2230, 2440
XbaI	TCTAGA	2 cut(s)	1330, 1720
XmiI	GTMKAC	1 cut(s)	403
XmnI	GAANNNNTTC	4 cut(s)	714, 961, 1055, 2312
XspI	CTAG	5 cut(s)	1026, 1331, 1721, 1967, 2502
ZrmI	AGTACT	1 cut(s)	421

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.