Rw2G049350

Plant protein of unknown function (DUF863)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0003069
Physical Location & Seq
Reverse (-)
76992181 .. 76996469
4289 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G049350.1

Sequence Viewer

Length: 1818 bp
ATGTCTGCGTCAACAAATGTGTACAGAGGTGTCTGGCCTTTTGCAAAGAAGTTCCCGGAGAACCCACAACTGGGAAAGGATGGAGGAGTTAGTAATCTGCATGGGAAGAATCAAAGCAACCAAAAGGAGTGGTTAACAAATGCTCTCAAGGCTGATCAAACAAGAAATTCTTTTGGTGGAAGTTTTGGCCTTCGAGATTCTCAGAAGCCTTGCGAACCATCAGAAACTGAAGCTAGGAAAGCATGTGAGCCTGTCAAGAGGACAATTTTTGGTATAGAAATCTCTGAAAGAGATATAAATCCATCTGGTAAGGCTTCTCAGTCTGATGTGGCCGCCATTTCTGAGTCACCTCCTACTTGGGAAAGACCTCCTAGTAGCTTGAGCCACAACCGGACATCAGGTCAATCGAACAGTTTGTTTAACACCTCCACACACTCTAATAGGGCTTCGATGATGTTGCAGCAAAACCCTGAAGTTATCAGGGACAGATTGCTCGTAGATTGCAATTCTATACCATTAACTCCAGCTCTGGGATCTGAAGTGTCACACCACAATGGTGTTTGCGTTCGCACCAAGATTGAGAGCAATGAATTACACAGTTATCACCCTTCAATCAGACTTGGCTTCCCCTATGCAGACAGCAGCTATAGTTCTGCTCCTAAAGATTTTACACAACATGTTCCTCAGTGGCAGTATTCGAGTGTAGGTTGCAGGGCGAATGCAAAGCCTTCACCTAATGTGCATGATAATAAATTTCTTCACAGACTGAGGAACCAAGAAACGCCACAAGGAAGCTTGCATTGGCTTGAGGCAGCTTCACTTTGTAATGGTCCACTTGCTTCAGCAGTTGACAATGACAATTCTGTTGGAGTTGGAGAGCAACTGAAAGCAGAGAGTTTATTTTCGGAAAAGGGATTGGATAATGACAATGCTTATTCAGGGCACCAAATAGACTTGAACAGATGTTTAACTGAAGAAGAAACTCGAATAGCTGTGCAGGCACCAGTAATAGTTGGAACAGATGTCAATGTTGGAGAAGCTTCTGAGGAAAGAAAATGTACAGAACTTTTTTATTCATCTCATGATGGTTTATTCAGGGTTGCAGCTGAGGCTCTCGTTGCCATCTCATCATCACAAGCTCATGAGATGCAGGAAAATGCTGCACATGGTCTTGATCAAGAAATTTCTGCTTGTCGTGAAGATGATGTAACAGTAATTGACGACTCCCTTCTTTGGTTTGCAGAAATAGTTTCTTCAAACGAAGAAAATCTTGAACAGACGGATAATGTGGTAAAAGTTAAGGGAGCGATGGATTACTTTGAGTACATGACATTAAATATGGCGGAAACCAAGTTAGAGCAGTGCTGCAATGTGCCTTTAGACAACCCAAAAGAAGTGCCGCTGTCAAAACGGCCACAAAGGGGTCAGACGAGGAAAGGGAGGCAACGAAAGGACTTCCAGAGGGATGTTCTCCCTGGTATTGCTTGTTTGTCAAGAAATGAGGTGACTGAGGATCTTCAAACATTTGAAGGGTCGATTAGAGCAAGTGGTGGCAGCTGGCAGTCAGGTTTGTCACAGAGAAATGCTGGTAAGGTTGGCGGGGGAAGAGGGCGGAAGCGTTTGGGAACTTGCGCACTACCTACTTCTAGTGCTGTGCAGGCTGCAGTTAGTCAGCCCCAGATGGAGCAGCAGCCTCAATGTGGGGAGCTGCAGGAACCTGGGGAAAGAAGTATGGCGGTGACAGGTTGGGGTAAGAGGACAAGGCGTCCACCGCGGCAAAGATATCCGATTAATAGTTCTCCTGTTTCTCAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF863

Protein Analysis

605

Amino Acids

66.56

Weight (kDa)

6.15

Isoelectric Point (pI)

51.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF863 PF05904 291 - 596 7e-68 Plant protein of unknown function (DUF863)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1633
AccB1I GGYRCC 2 cut(s) 942, 1000
AccII CGCG 1 cut(s) 1774
AciI CCGC 8 cut(s) 333, 1343, 1400, 1599, 1612, 1736, 1772, 1774
AclWI GGATC 2 cut(s) 541, 1521
AcoI YGGCCR 2 cut(s) 330, 1412
AcsI RAATTY 3 cut(s) 166, 752, 1182
AcuI CTGAAG 5 cut(s) 249, 492, 558, 825, 993
AcyI GRCGYC 1 cut(s) 1765
AfaI GTAC 3 cut(s) 23, 1060, 1325
AfiI CCNNNNNNNGG 6 cut(s) 70, 71, 530, 1233, 1421, 1700
AflIII ACRYGT 1 cut(s) 676
AgsI TTSAA 6 cut(s) 612, 958, 1257, 1274, 1520, 1529
AhdI GACNNNNNGTC 2 cut(s) 399, 1764
AjnI CCWGG 2 cut(s) 1474, 1717
AjuI GAANNNNNNNTTGG 2 cut(s) 784, 816
AleI CACNNNNGTG 1 cut(s) 555
AlwI GGATC 2 cut(s) 541, 1521
AlwNI CAGNNNCTG 1 cut(s) 227
AoxI GGCC 4 cut(s) 35, 187, 330, 1412
ApoI RAATTY 3 cut(s) 166, 752, 1182
AseI ATTAAT 1 cut(s) 1791
AspLEI GCGC 1 cut(s) 1634
AspS9I GGNCC 1 cut(s) 830
AsuC2I CCSGG 1 cut(s) 56
AsuHPI GGTGA 5 cut(s) 339, 596, 723, 1516, 1750
AvaII GGWCC 1 cut(s) 830
BaeGI GKGCMC 1 cut(s) 945
BanI GGYRCC 2 cut(s) 942, 1000
BbvCI CCTCAGC 1 cut(s) 1107
BccI CCATC 7 cut(s) 74, 226, 310, 1079, 1130, 1303, 1675
BceAI ACGGC 1 cut(s) 1427
BcgI CGANNNNNNTGC 2 cut(s) 439, 473
BciT130I CCWGG 2 cut(s) 1476, 1719
BclI TGATCA 2 cut(s) 154, 1174
BcnI CCSGG 1 cut(s) 56
BfaI CTAG 3 cut(s) 234, 372, 1647
BfmI CTRYAG 3 cut(s) 646, 1662, 1709
Bme1390I CCNGG 3 cut(s) 56, 1476, 1719
Bme18I GGWCC 1 cut(s) 830
BmeRI GACNNNNNGTC 2 cut(s) 399, 1764
BmgT120I GGNCC 1 cut(s) 830
BmiI GGNNCC 4 cut(s) 773, 944, 1002, 1716
BmrFI CCNGG 3 cut(s) 56, 1476, 1719
BmrI ACTGGG 1 cut(s) 80
BmsI GCATC 1 cut(s) 1137
BmuI ACTGGG 1 cut(s) 80
BpmI CTGGAG 1 cut(s) 507
Bpu10I CCTNAGC 1 cut(s) 1107
BpuEI CTTGAG 3 cut(s) 131, 400, 827
BpuMI CCSGG 1 cut(s) 56
BsaBI GATNNNNATC 1 cut(s) 297
BsaHI GRCGYC 1 cut(s) 1765
BsaJI CCNNGG 3 cut(s) 1474, 1718, 1772
BsaWI WCCGGW 1 cut(s) 390
Bsc4I CCNNNNNNNGG 6 cut(s) 70, 71, 530, 1233, 1421, 1700
Bse1I ACTGG 2 cut(s) 75, 1004
Bse3DI GCAATG 2 cut(s) 592, 1375
Bse8I GATNNNNATC 1 cut(s) 297
BseBI CCWGG 2 cut(s) 1476, 1719
BseDI CCNNGG 3 cut(s) 1474, 1718, 1772
BseGI GGATG 2 cut(s) 85, 1471
BseJI GATNNNNATC 1 cut(s) 297
BseLI CCNNNNNNNGG 6 cut(s) 70, 71, 530, 1233, 1421, 1700
BseMI GCAATG 2 cut(s) 592, 1375
BseMII CTCAG 8 cut(s) 215, 332, 333, 698, 758, 1035, 1098, 1500
BseNI ACTGG 2 cut(s) 75, 1004
BseRI GAGGAG 1 cut(s) 99
BseSI GKGCMC 1 cut(s) 945
BsgI GTGCAG 3 cut(s) 1016, 1146, 1676
Bsh1236I CGCG 1 cut(s) 1774
BshFI GGCC 4 cut(s) 37, 189, 332, 1414
BshNI GGYRCC 2 cut(s) 942, 1000
BsiSI CCGG 2 cut(s) 56, 391
BslFI GGGAC 1 cut(s) 497
BslI CCNNNNNNNGG 6 cut(s) 70, 71, 530, 1233, 1421, 1700
BsmFI GGGAC 1 cut(s) 497
BsmI GAATGC 1 cut(s) 724
BsnI GGCC 4 cut(s) 37, 189, 332, 1414
Bsp1286I GDGCHC 1 cut(s) 945
Bsp1407I TGTACA 2 cut(s) 21, 1058
Bsp143I GATC 4 cut(s) 154, 533, 1174, 1513
BspACI CCGC 8 cut(s) 333, 1343, 1400, 1599, 1612, 1736, 1772, 1774
BspANI GGCC 4 cut(s) 37, 189, 332, 1414
BspCNI CTCAG 8 cut(s) 214, 331, 334, 697, 759, 1036, 1099, 1501
BspFNI CGCG 1 cut(s) 1774
BspHI TCATGA 2 cut(s) 1081, 1141
BspLI GGNNCC 4 cut(s) 773, 944, 1002, 1716
BspMAI CTGCAG 2 cut(s) 1666, 1713
BspPI GGATC 2 cut(s) 541, 1521
BspT107I GGYRCC 2 cut(s) 942, 1000
BsrDI GCAATG 2 cut(s) 592, 1375
BsrGI TGTACA 2 cut(s) 21, 1058
BsrI ACTGG 2 cut(s) 75, 1004
BssECI CCNNGG 3 cut(s) 1474, 1718, 1772
BssMI GATC 4 cut(s) 154, 533, 1174, 1513
BssNI GRCGYC 1 cut(s) 1765
Bst2UI CCWGG 2 cut(s) 1476, 1719
Bst4CI ACNGT 3 cut(s) 413, 599, 1213
Bst6I CTCTTC 1 cut(s) 1600
BstACI GRCGYC 1 cut(s) 1765
BstAUI TGTACA 2 cut(s) 21, 1058
BstC8I GCNNGC 4 cut(s) 797, 999, 1559, 1659
BstDEI CTNAG 8 cut(s) 201, 318, 342, 684, 767, 1044, 1107, 1509
BstDSI CCRYGG 1 cut(s) 1772
BstF5I GGATG 2 cut(s) 85, 1471
BstFNI CGCG 1 cut(s) 1774
BstHHI GCGC 1 cut(s) 1634
BstKTI GATC 4 cut(s) 157, 536, 1177, 1516
BstMBI GATC 4 cut(s) 154, 533, 1174, 1513
BstMWI GCNNNNNNNGC 7 cut(s) 149, 239, 998, 1109, 1118, 1658, 1771
BstNI CCWGG 2 cut(s) 1476, 1719
BstNSI RCATGY 2 cut(s) 246, 680
BstSCI CCNGG 3 cut(s) 54, 1474, 1717
BstSFI CTRYAG 3 cut(s) 646, 1662, 1709
BstSLI GKGCMC 1 cut(s) 945
BstUI CGCG 1 cut(s) 1774
BstX2I RGATCY 2 cut(s) 533, 1513
BstYI RGATCY 2 cut(s) 533, 1513
BsuRI GGCC 4 cut(s) 37, 189, 332, 1414
BtgI CCRYGG 1 cut(s) 1772
BtgZI GCGATG 1 cut(s) 1322
BtsCI GGATG 2 cut(s) 85, 1471
BtsI GCAGTG 1 cut(s) 1367
BtsIMutI CAGTG 2 cut(s) 692, 1367
Cac8I GCNNGC 4 cut(s) 797, 999, 1559, 1659
CaiI CAGNNNCTG 1 cut(s) 227
CciI TCATGA 2 cut(s) 1081, 1141
CfoI GCGC 1 cut(s) 1634
Cfr13I GGNCC 1 cut(s) 830
Cfr42I CCGCGG 1 cut(s) 1775
CseI GACGC 1 cut(s) 1754
Csp6I GTAC 3 cut(s) 22, 1059, 1324
CspCI CAANNNNNGTGG 2 cut(s) 110, 145
CviAII CATG 8 cut(s) 101, 243, 677, 743, 1082, 1142, 1166, 1327
CviQI GTAC 3 cut(s) 22, 1059, 1324
DdeI CTNAG 8 cut(s) 201, 318, 342, 684, 767, 1044, 1107, 1509
DpnI GATC 4 cut(s) 156, 535, 1176, 1515
DpnII GATC 4 cut(s) 154, 533, 1174, 1513
DriI GACNNNNNGTC 2 cut(s) 399, 1764
EaeI YGGCCR 2 cut(s) 330, 1412
Eam1104I CTCTTC 1 cut(s) 1600
Eam1105I GACNNNNNGTC 2 cut(s) 399, 1764
EarI CTCTTC 1 cut(s) 1600
EciI GGCGGA 2 cut(s) 1358, 1627
Eco32I GATATC 1 cut(s) 1784
Eco47I GGWCC 1 cut(s) 830
Eco57I CTGAAG 5 cut(s) 249, 492, 558, 825, 993
EcoRII CCWGG 2 cut(s) 1474, 1717
EcoRV GATATC 1 cut(s) 1784
FaeI CATG 8 cut(s) 104, 246, 680, 746, 1085, 1145, 1169, 1330
FalI AAGNNNNNCTT 4 cut(s) 154, 186, 1254, 1286
FaqI GGGAC 1 cut(s) 497
FatI CATG 8 cut(s) 100, 242, 676, 742, 1081, 1141, 1165, 1326
FauI CCCGC 1 cut(s) 1592
FbaI TGATCA 2 cut(s) 154, 1174
FokI GGATG 2 cut(s) 92, 1478
FspBI CTAG 3 cut(s) 234, 372, 1647
FspI TGCGCA 1 cut(s) 1633
GlaI GCGC 1 cut(s) 1633
GsuI CTGGAG 1 cut(s) 507
HaeIII GGCC 4 cut(s) 37, 189, 332, 1414
HapII CCGG 2 cut(s) 56, 391
HgaI GACGC 1 cut(s) 1754
HhaI GCGC 1 cut(s) 1634
Hin1I GRCGYC 1 cut(s) 1765
Hin1II CATG 8 cut(s) 104, 246, 680, 746, 1085, 1145, 1169, 1330
Hin6I GCGC 1 cut(s) 1632
HinP1I GCGC 1 cut(s) 1632
HincII GTYRAC 3 cut(s) 12, 135, 850
HindII GTYRAC 3 cut(s) 12, 135, 850
HindIII AAGCTT 2 cut(s) 793, 1038
HinfI GANTC 4 cut(s) 109, 197, 344, 1223
HpaI GTTAAC 1 cut(s) 135
HpaII CCGG 2 cut(s) 56, 391
HphI GGTGA 5 cut(s) 339, 596, 723, 1516, 1750
Hpy166II GTNNAC 6 cut(s) 12, 22, 135, 833, 850, 1769
Hpy8I GTNNAC 6 cut(s) 12, 22, 135, 833, 850, 1769
HpyAV CCTTC 5 cut(s) 200, 618, 738, 1238, 1523
HpyCH4III ACNGT 3 cut(s) 413, 599, 1213
HpyF10VI GCNNNNNNNGC 7 cut(s) 149, 239, 998, 1109, 1118, 1658, 1771
HpyF3I CTNAG 8 cut(s) 201, 318, 342, 684, 767, 1044, 1107, 1509
Hsp92I GRCGYC 1 cut(s) 1765
Hsp92II CATG 8 cut(s) 104, 246, 680, 746, 1085, 1145, 1169, 1330
HspAI GCGC 1 cut(s) 1632
Ksp22I TGATCA 2 cut(s) 154, 1174
KspAI GTTAAC 1 cut(s) 135
KspI CCGCGG 1 cut(s) 1775
Kzo9I GATC 4 cut(s) 154, 533, 1174, 1513
LmnI GCTCC 4 cut(s) 661, 1304, 1684, 1705
LweI GCATC 1 cut(s) 1137
MaeI CTAG 3 cut(s) 234, 372, 1647
MaeIII GTNAC 6 cut(s) 345, 543, 1207, 1504, 1572, 1738
MalI GATC 4 cut(s) 156, 535, 1176, 1515
MboI GATC 4 cut(s) 154, 533, 1174, 1513
MboII GAAGA 9 cut(s) 118, 749, 986, 989, 1211, 1245, 1274, 1508, 1617
MflI RGATCY 2 cut(s) 533, 1513
MhlI GDGCHC 1 cut(s) 945
MluCI AATT 8 cut(s) 166, 264, 505, 590, 752, 859, 1182, 1215
MlyI GAGTC 2 cut(s) 353, 1217
MmeI TCCRAC 4 cut(s) 847, 853, 994, 1012
MseI TTAA 7 cut(s) 134, 420, 518, 968, 1299, 1334, 1791
MslI CAYNNNNRTG 2 cut(s) 552, 555
MspA1I CMGCKG 4 cut(s) 1106, 1402, 1557, 1774
MspI CCGG 2 cut(s) 56, 391
MspR9I CCNGG 3 cut(s) 56, 1476, 1719
Mva1269I GAATGC 1 cut(s) 724
MvaI CCWGG 2 cut(s) 1476, 1719
MvnI CGCG 1 cut(s) 1774
MwoI GCNNNNNNNGC 7 cut(s) 149, 239, 998, 1109, 1118, 1658, 1771
NciI CCSGG 1 cut(s) 56
NdeII GATC 4 cut(s) 154, 533, 1174, 1513
NlaIII CATG 8 cut(s) 104, 246, 680, 746, 1085, 1145, 1169, 1330
NlaIV GGNNCC 4 cut(s) 773, 944, 1002, 1716
NmuCI GTSAC 5 cut(s) 345, 543, 1504, 1572, 1738
NsbI TGCGCA 1 cut(s) 1633
NspI RCATGY 2 cut(s) 246, 680
OliI CACNNNNGTG 1 cut(s) 555
PagI TCATGA 2 cut(s) 1081, 1141
PciI ACATGT 1 cut(s) 676
PctI GAATGC 1 cut(s) 724
PfeI GAWTC 2 cut(s) 109, 197
PfoI TCCNGGA 1 cut(s) 54
PleI GAGTC 2 cut(s) 352, 1217
PpsI GAGTC 2 cut(s) 352, 1217
PscI ACATGT 1 cut(s) 676
PshBI ATTAAT 1 cut(s) 1791
Psp6I CCWGG 2 cut(s) 1474, 1717
PspGI CCWGG 2 cut(s) 1474, 1717
PspN4I GGNNCC 4 cut(s) 773, 944, 1002, 1716
PspPI GGNCC 1 cut(s) 830
PstI CTGCAG 2 cut(s) 1666, 1713
PstNI CAGNNNCTG 1 cut(s) 227
PsuI RGATCY 2 cut(s) 533, 1513
PvuII CAGCTG 2 cut(s) 1106, 1557
RsaI GTAC 3 cut(s) 23, 1060, 1325
RsaNI GTAC 3 cut(s) 22, 1059, 1324
RseI CAYNNNNRTG 2 cut(s) 552, 555
SacII CCGCGG 1 cut(s) 1775
SaqAI TTAA 7 cut(s) 134, 420, 518, 968, 1299, 1334, 1791
Sau3AI GATC 4 cut(s) 154, 533, 1174, 1513
Sau96I GGNCC 1 cut(s) 830
SchI GAGTC 2 cut(s) 353, 1217
ScrFI CCNGG 3 cut(s) 56, 1476, 1719
SduI GDGCHC 1 cut(s) 945
SfaNI GCATC 1 cut(s) 1137
SfcI CTRYAG 3 cut(s) 646, 1662, 1709
Sfr303I CCGCGG 1 cut(s) 1775
SgrBI CCGCGG 1 cut(s) 1775
SinI GGWCC 1 cut(s) 830
SmiMI CAYNNNNRTG 2 cut(s) 552, 555
SmlI CTYRAG 3 cut(s) 146, 379, 806
SmoI CTYRAG 3 cut(s) 146, 379, 806
Sse9I AATT 8 cut(s) 166, 264, 505, 590, 752, 859, 1182, 1215
SsiI CCGC 8 cut(s) 333, 1343, 1400, 1599, 1612, 1736, 1772, 1774
SspMI CTAG 3 cut(s) 234, 372, 1647
StyD4I CCNGG 3 cut(s) 54, 1474, 1717
TaaI ACNGT 3 cut(s) 413, 599, 1213
TaqI TCGA 6 cut(s) 193, 407, 449, 698, 985, 1535
TasI AATT 8 cut(s) 166, 264, 505, 590, 752, 859, 1182, 1215
TatI WGTACW 3 cut(s) 21, 1058, 1323
TauI GCSGC 3 cut(s) 335, 1402, 1777
TfiI GAWTC 2 cut(s) 109, 197
Tru1I TTAA 7 cut(s) 134, 420, 518, 968, 1299, 1334, 1791
Tru9I TTAA 7 cut(s) 134, 420, 518, 968, 1299, 1334, 1791
TscAI CASTG 2 cut(s) 692, 1367
TseFI GTSAC 5 cut(s) 345, 543, 1504, 1572, 1738
Tsp45I GTSAC 5 cut(s) 345, 543, 1504, 1572, 1738
TspDTI ATGAA 2 cut(s) 603, 1065
TspGWI ACGGA 1 cut(s) 1295
TspRI CASTG 2 cut(s) 692, 1367
VpaK11BI GGWCC 1 cut(s) 830
VspI ATTAAT 1 cut(s) 1791
XapI RAATTY 3 cut(s) 166, 752, 1182
XceI RCATGY 2 cut(s) 246, 680
XspI CTAG 3 cut(s) 234, 372, 1647
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.