RLG00000021662

Plant protein of unknown function (DUF863)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0003069
Physical Location & Seq
Reverse (-)
77085817 .. 77087924
2108 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021662

Sequence Viewer

Length: 1746 bp
ATGTTTATCGAGTTAATTGCAGGAATGGGAACTGAAGTACAGTCAAAGATGTATCTACCAGGATATTGTTCCATACCAACTCTAACTAACAATGCTGGCCATGGTGGATGGTCCCTACTCCACGAGAACAAAACCTTGAAGAATGGGCAACAATATGACATACTCTCAACGAGGCCAGTTGTTGATGGATTTCATGGATGCAGTAAGGAACAAGTGAGGCAAATGATATTGAAGCATGAATCCATTTTCAAACATCAGCTCAATGAACTTCATCGTCTTTATGAAAGACAAAAGGACTTGAAGAATAGATCAAAAGCAAAGAACTGCTCAAACATCAAAAAGAAGCAGCGACATCACAATTACCTCTTTTTTCATCTGCAAACTAGTGGTGGCCCCAGCCAAAGTGGAGTCAAGAAGCTTCAGAGAAGATTATTCAACCTTGAACTTCCAGCTGACGAATACATCAGTGATGGAGAAGAACCAGGTGTTGTTTGCTTGGGACCAGCAACCGAAAATTATCCTCCTATCAGAAGAAATGAGGCTAAAGTGTCATTTAATCGCATTGGTGAAGCATCTGGCAATAACAATGGTGCGTTGAGCTCTGGCTTGTATCTAGAAAGAAGCTATTGTTTGACCACTCAGAATGAACCCATTCAGATTGATAAAATGTCTGCCTCAACAAATGTGTACAAAGGTGTCTGGCCTTTTGCAAAGAAGTTTCCGGAGAACCCACAACTGGGAAAGGATGGTGGGGTTAGTAATCTGCATGTGAAGAATGACAGCAACCAAAAGGTGAATGCAAAGCCTTCACCTGAAGTGCATGATAATAAATTTCTTCGCAGACTGAGGAACCAAGAAACGCCACAAGGAAGATTGCATTGGCTTCAGGCAGCTTCACTTTGTAATGGTCCACTTGCTTCAGCAGTTGACGATGACAATTCTGTTGGAGTTGGAGAGCAACTGAAAGCAGAGAGTTTATTTCCGGAAAAGGGATTGGATAATTACAATGCTTATTCAGGGCACCAAATAGACTTGAACAGATGTTTAACTGAAGAAGAAACTCGAATAGCTGTGCAGGCACAAGTAATAGTTGCAACAGATGTCAATGTTGGAGAAGCTTCTGAGGAAAGAAAGTGTAAAGAACTTTTTGATTCATCTCATGATGGTTTATTCAGGGTTGCAGCTGAGGCTTTGGTTGCCATCTCATCATCACAAGCTCATGAGATGCTGGAAAATACTGCACATGGTCTTGATCAAGAAATTTCTGCTTGTTGTGAAGATGATGCAGCGTGCTGCAATGTGCCTTCAGACAACCCAAAAGAAGTGCCACTGTCAAAACGGCCACAAAGGGGTCAGACGAGGAGAGGGAGGCAACGAAAGGACTTCCAGAGAGATGTTGTCCCTGGTATTGCTTGTTTGCCAAGAAATGAGGTGACTGAGGATCTTCAAACATTTGAAGGGTCGATTAGAGCAAGTGGTGGCAGCTGGCAGTCAGATTTATCGCAGAGAAATGCTGGTAAGGTTGGCGGGGGAAGAGGGAGGAAGCGTTTGGGAACTTGCGCCCTAGCTACTTCTAGTACTGTGCAGGCTGCAGTTAGTCAGCCCCAGATGGAGCAGCAGCCTCAATGTGGGGAGCTGCAGGAACCTGAGGAGAGAAGTATGGCATTGACAGTTTGGGGTAAGAGGACAAGGCGTCCACCACGGCAAAGATATCCGATTAATAGTTCTCCCGTTTCTCAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF863

Protein Analysis

582

Amino Acids

64.57

Weight (kDa)

8.59

Isoelectric Point (pI)

55.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF863 PF05904 444 - 572 4.3e-40 Plant protein of unknown function (DUF863)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1020
AccIII TCCGGA 2 cut(s) 721, 982
AciI CCGC 1 cut(s) 1527
AclWI GGATC 1 cut(s) 1449
AcoI YGGCCR 2 cut(s) 97, 1340
AcsI RAATTY 2 cut(s) 830, 1260
AcuI CTGAAG 7 cut(s) 54, 404, 834, 869, 903, 1071, 1290
AcyI GRCGYC 1 cut(s) 1693
AfaI GTAC 3 cut(s) 39, 689, 1579
AfiI CCNNNNNNNGG 5 cut(s) 736, 737, 989, 1349, 1628
AgsI TTSAA 9 cut(s) 139, 232, 250, 301, 436, 443, 1036, 1448, 1457
AhdI GACNNNNNGTC 1 cut(s) 1692
AhlI ACTAGT 1 cut(s) 383
AjnI CCWGG 3 cut(s) 58, 481, 1402
AjuI GAANNNNNNNTTGG 2 cut(s) 862, 894
Alw21I GWGCWC 1 cut(s) 602
AlwI GGATC 1 cut(s) 1449
Aor13HI TCCGGA 2 cut(s) 721, 982
AoxI GGCC 5 cut(s) 97, 173, 391, 701, 1340
ApoI RAATTY 2 cut(s) 830, 1260
AseI ATTAAT 1 cut(s) 1719
Asp700I GAANNNNTTC 1 cut(s) 651
AspLEI GCGC 1 cut(s) 1562
AspS9I GGNCC 4 cut(s) 111, 392, 500, 908
AsuHPI GGTGA 4 cut(s) 578, 801, 805, 1444
AvaII GGWCC 3 cut(s) 111, 500, 908
AxyI CCTNAGG 1 cut(s) 1647
BaeGI GKGCMC 1 cut(s) 1023
BalI TGGCCA 1 cut(s) 99
BanI GGYRCC 1 cut(s) 1020
BanII GRGCYC 1 cut(s) 602
BauI CACGAG 1 cut(s) 122
Bbv12I GWGCWC 1 cut(s) 602
BbvCI CCTCAGC 1 cut(s) 1185
BccI CCATC 7 cut(s) 102, 179, 464, 740, 1157, 1208, 1603
BceAI ACGGC 2 cut(s) 1355, 1718
BcgI CGANNNNNNTGC 1 cut(s) 33
BciT130I CCWGG 3 cut(s) 60, 483, 1404
BclI TGATCA 1 cut(s) 1252
BcuI ACTAGT 1 cut(s) 383
BfaI CTAG 4 cut(s) 384, 614, 1565, 1575
BfmI CTRYAG 2 cut(s) 1590, 1637
BmcAI AGTACT 1 cut(s) 1579
Bme1390I CCNGG 3 cut(s) 60, 483, 1404
Bme18I GGWCC 3 cut(s) 111, 500, 908
BmeRI GACNNNNNGTC 1 cut(s) 1692
BmgT120I GGNCC 4 cut(s) 111, 392, 500, 908
BmiI GGNNCC 6 cut(s) 113, 394, 501, 851, 1022, 1644
BmrFI CCNGG 3 cut(s) 60, 483, 1404
BmrI ACTGGG 1 cut(s) 746
BmsI GCATC 4 cut(s) 188, 581, 1215, 1273
BmuI ACTGGG 1 cut(s) 746
Bpu10I CCTNAGC 1 cut(s) 1185
BsaHI GRCGYC 1 cut(s) 1693
BsaJI CCNNGG 3 cut(s) 100, 1402, 1700
BsaWI WCCGGW 2 cut(s) 721, 982
BsaXI ACNNNNNCTCC 2 cut(s) 1531, 1561
Bsc4I CCNNNNNNNGG 5 cut(s) 736, 737, 989, 1349, 1628
Bse1I ACTGG 2 cut(s) 176, 741
Bse21I CCTNAGG 1 cut(s) 1647
Bse3DI GCAATG 1 cut(s) 1303
BseAI TCCGGA 2 cut(s) 721, 982
BseBI CCWGG 3 cut(s) 60, 483, 1404
BseDI CCNNGG 3 cut(s) 100, 1402, 1700
BseGI GGATG 3 cut(s) 113, 203, 751
BseLI CCNNNNNNNGG 5 cut(s) 736, 737, 989, 1349, 1628
BseMI GCAATG 1 cut(s) 1303
BseMII CTCAG 6 cut(s) 653, 836, 1113, 1176, 1428, 1638
BseNI ACTGG 2 cut(s) 176, 741
BseRI GAGGAG 2 cut(s) 1375, 1664
BseSI GKGCMC 1 cut(s) 1023
BseYI CCCAGC 1 cut(s) 395
BsgI GTGCAG 3 cut(s) 1094, 1224, 1604
BshFI GGCC 5 cut(s) 99, 175, 393, 703, 1342
BshNI GGYRCC 1 cut(s) 1020
BsiHKAI GWGCWC 1 cut(s) 602
BsiSI CCGG 2 cut(s) 722, 983
BslFI GGGAC 3 cut(s) 97, 513, 1385
BslI CCNNNNNNNGG 5 cut(s) 736, 737, 989, 1349, 1628
BsmFI GGGAC 3 cut(s) 97, 513, 1385
BsmI GAATGC 1 cut(s) 802
BsnI GGCC 5 cut(s) 99, 175, 393, 703, 1342
Bsp1286I GDGCHC 2 cut(s) 602, 1023
Bsp13I TCCGGA 2 cut(s) 721, 982
Bsp1407I TGTACA 1 cut(s) 687
Bsp143I GATC 3 cut(s) 308, 1252, 1441
Bsp19I CCATGG 1 cut(s) 100
BspACI CCGC 1 cut(s) 1527
BspANI GGCC 5 cut(s) 99, 175, 393, 703, 1342
BspCNI CTCAG 6 cut(s) 652, 837, 1114, 1177, 1429, 1639
BspEI TCCGGA 2 cut(s) 721, 982
BspHI TCATGA 2 cut(s) 1159, 1219
BspLI GGNNCC 6 cut(s) 113, 394, 501, 851, 1022, 1644
BspMAI CTGCAG 2 cut(s) 1594, 1641
BspPI GGATC 1 cut(s) 1449
BspT107I GGYRCC 1 cut(s) 1020
BsrDI GCAATG 1 cut(s) 1303
BsrGI TGTACA 1 cut(s) 687
BsrI ACTGG 2 cut(s) 176, 741
BssECI CCNNGG 3 cut(s) 100, 1402, 1700
BssMI GATC 3 cut(s) 308, 1252, 1441
BssNI GRCGYC 1 cut(s) 1693
BssSI CACGAG 1 cut(s) 122
BssT1I CCWWGG 1 cut(s) 100
Bst2BI CACGAG 1 cut(s) 122
Bst2UI CCWGG 3 cut(s) 60, 483, 1404
Bst4CI ACNGT 4 cut(s) 42, 1332, 1582, 1672
Bst6I CTCTTC 1 cut(s) 1528
BstACI GRCGYC 1 cut(s) 1693
BstAUI TGTACA 1 cut(s) 687
BstC8I GCNNGC 5 cut(s) 97, 1077, 1291, 1487, 1587
BstDEI CTNAG 6 cut(s) 639, 845, 1122, 1185, 1437, 1647
BstDSI CCRYGG 2 cut(s) 100, 1700
BstF5I GGATG 3 cut(s) 113, 203, 751
BstHHI GCGC 1 cut(s) 1562
BstKTI GATC 3 cut(s) 311, 1255, 1444
BstMBI GATC 3 cut(s) 308, 1252, 1441
BstMWI GCNNNNNNNGC 3 cut(s) 1076, 1187, 1196
BstNI CCWGG 3 cut(s) 60, 483, 1404
BstNSI RCATGY 1 cut(s) 770
BstSCI CCNGG 3 cut(s) 58, 481, 1402
BstSFI CTRYAG 2 cut(s) 1590, 1637
BstSLI GKGCMC 1 cut(s) 1023
BstX2I RGATCY 1 cut(s) 1441
BstYI RGATCY 1 cut(s) 1441
Bsu36I CCTNAGG 1 cut(s) 1647
BsuRI GGCC 5 cut(s) 99, 175, 393, 703, 1342
BtgI CCRYGG 2 cut(s) 100, 1700
BtsCI GGATG 3 cut(s) 113, 203, 751
BtsIMutI CAGTG 2 cut(s) 472, 1328
Cac8I GCNNGC 5 cut(s) 97, 1077, 1291, 1487, 1587
CciI TCATGA 2 cut(s) 1159, 1219
CfoI GCGC 1 cut(s) 1562
Cfr13I GGNCC 4 cut(s) 111, 392, 500, 908
CseI GACGC 1 cut(s) 1682
CsiI ACCWGGT 1 cut(s) 481
Csp6I GTAC 3 cut(s) 38, 688, 1578
CviAII CATG 8 cut(s) 101, 194, 236, 767, 821, 1160, 1220, 1244
CviQI GTAC 3 cut(s) 38, 688, 1578
DdeI CTNAG 6 cut(s) 639, 845, 1122, 1185, 1437, 1647
DpnI GATC 3 cut(s) 310, 1254, 1443
DpnII GATC 3 cut(s) 308, 1252, 1441
DriI GACNNNNNGTC 1 cut(s) 1692
EaeI YGGCCR 2 cut(s) 97, 1340
Eam1104I CTCTTC 1 cut(s) 1528
Eam1105I GACNNNNNGTC 1 cut(s) 1692
EarI CTCTTC 1 cut(s) 1528
Ecl136II GAGCTC 1 cut(s) 600
Eco130I CCWWGG 1 cut(s) 100
Eco24I GRGCYC 1 cut(s) 602
Eco32I GATATC 1 cut(s) 1712
Eco47I GGWCC 3 cut(s) 111, 500, 908
Eco53kI GAGCTC 1 cut(s) 600
Eco57I CTGAAG 7 cut(s) 54, 404, 834, 869, 903, 1071, 1290
Eco81I CCTNAGG 1 cut(s) 1647
EcoICRI GAGCTC 1 cut(s) 600
EcoRII CCWGG 3 cut(s) 58, 481, 1402
EcoRV GATATC 1 cut(s) 1712
EcoT14I CCWWGG 1 cut(s) 100
EcoT38I GRGCYC 1 cut(s) 602
ErhI CCWWGG 1 cut(s) 100
FaeI CATG 8 cut(s) 104, 197, 239, 770, 824, 1163, 1223, 1247
FaqI GGGAC 3 cut(s) 97, 513, 1385
FatI CATG 8 cut(s) 100, 193, 235, 766, 820, 1159, 1219, 1243
FauI CCCGC 1 cut(s) 1520
FbaI TGATCA 1 cut(s) 1252
FokI GGATG 3 cut(s) 120, 210, 758
FriOI GRGCYC 1 cut(s) 602
FspBI CTAG 4 cut(s) 384, 614, 1565, 1575
GlaI GCGC 1 cut(s) 1561
GsaI CCCAGC 1 cut(s) 399
HaeIII GGCC 5 cut(s) 99, 175, 393, 703, 1342
HapII CCGG 2 cut(s) 722, 983
HgaI GACGC 1 cut(s) 1682
HhaI GCGC 1 cut(s) 1562
Hin1I GRCGYC 1 cut(s) 1693
Hin1II CATG 8 cut(s) 104, 197, 239, 770, 824, 1163, 1223, 1247
Hin6I GCGC 1 cut(s) 1560
HinP1I GCGC 1 cut(s) 1560
HincII GTYRAC 1 cut(s) 928
HindII GTYRAC 1 cut(s) 928
HindIII AAGCTT 2 cut(s) 416, 1116
HinfI GANTC 3 cut(s) 239, 408, 1151
HpaII CCGG 2 cut(s) 722, 983
HphI GGTGA 4 cut(s) 578, 801, 805, 1444
Hpy166II GTNNAC 4 cut(s) 688, 911, 928, 1697
Hpy188I TCNGA 9 cut(s) 423, 530, 642, 657, 1123, 1309, 1356, 1495, 1716
Hpy188III TCNNGA 9 cut(s) 412, 614, 722, 983, 1160, 1220, 1250, 1256, 1387
Hpy8I GTNNAC 4 cut(s) 688, 911, 928, 1697
HpyAV CCTTC 3 cut(s) 816, 1314, 1451
HpyCH4III ACNGT 4 cut(s) 42, 1332, 1582, 1672
HpyF10VI GCNNNNNNNGC 3 cut(s) 1076, 1187, 1196
HpyF3I CTNAG 6 cut(s) 639, 845, 1122, 1185, 1437, 1647
Hsp92I GRCGYC 1 cut(s) 1693
Hsp92II CATG 8 cut(s) 104, 197, 239, 770, 824, 1163, 1223, 1247
HspAI GCGC 1 cut(s) 1560
Kpn2I TCCGGA 2 cut(s) 721, 982
Ksp22I TGATCA 1 cut(s) 1252
Kzo9I GATC 3 cut(s) 308, 1252, 1441
LmnI GCTCC 2 cut(s) 1612, 1633
LweI GCATC 4 cut(s) 188, 581, 1215, 1273
MabI ACCWGGT 1 cut(s) 481
MaeI CTAG 4 cut(s) 384, 614, 1565, 1575
MaeIII GTNAC 1 cut(s) 1432
MalI GATC 3 cut(s) 310, 1254, 1443
MboI GATC 3 cut(s) 308, 1252, 1441
MflI RGATCY 1 cut(s) 1441
MhlI GDGCHC 2 cut(s) 602, 1023
MlsI TGGCCA 1 cut(s) 99
MluCI AATT 7 cut(s) 15, 358, 514, 830, 937, 1000, 1260
MluNI TGGCCA 1 cut(s) 99
MlyI GAGTC 1 cut(s) 417
MmeI TCCRAC 3 cut(s) 925, 931, 1090
Mox20I TGGCCA 1 cut(s) 99
MroI TCCGGA 2 cut(s) 721, 982
MroXI GAANNNNTTC 1 cut(s) 651
MscI TGGCCA 1 cut(s) 99
MseI TTAA 4 cut(s) 14, 555, 1046, 1719
Msp20I TGGCCA 1 cut(s) 99
MspA1I CMGCKG 3 cut(s) 452, 1184, 1485
MspI CCGG 2 cut(s) 722, 983
MspR9I CCNGG 3 cut(s) 60, 483, 1404
Mva1269I GAATGC 1 cut(s) 802
MvaI CCWGG 3 cut(s) 60, 483, 1404
MwoI GCNNNNNNNGC 3 cut(s) 1076, 1187, 1196
NcoI CCATGG 1 cut(s) 100
NdeII GATC 3 cut(s) 308, 1252, 1441
NlaIII CATG 8 cut(s) 104, 197, 239, 770, 824, 1163, 1223, 1247
NlaIV GGNNCC 6 cut(s) 113, 394, 501, 851, 1022, 1644
NmuCI GTSAC 1 cut(s) 1432
NspI RCATGY 1 cut(s) 770
PagI TCATGA 2 cut(s) 1159, 1219
PctI GAATGC 1 cut(s) 802
PdmI GAANNNNTTC 1 cut(s) 651
PfeI GAWTC 2 cut(s) 239, 1151
PleI GAGTC 1 cut(s) 416
PpsI GAGTC 1 cut(s) 416
PshBI ATTAAT 1 cut(s) 1719
Psp124BI GAGCTC 1 cut(s) 602
Psp6I CCWGG 3 cut(s) 58, 481, 1402
PspFI CCCAGC 1 cut(s) 395
PspGI CCWGG 3 cut(s) 58, 481, 1402
PspN4I GGNNCC 6 cut(s) 113, 394, 501, 851, 1022, 1644
PspPI GGNCC 4 cut(s) 111, 392, 500, 908
PstI CTGCAG 2 cut(s) 1594, 1641
PsuI RGATCY 1 cut(s) 1441
PvuII CAGCTG 3 cut(s) 452, 1184, 1485
RsaI GTAC 3 cut(s) 39, 689, 1579
RsaNI GTAC 3 cut(s) 38, 688, 1578
SacI GAGCTC 1 cut(s) 602
SaqAI TTAA 4 cut(s) 14, 555, 1046, 1719
Sau3AI GATC 3 cut(s) 308, 1252, 1441
Sau96I GGNCC 4 cut(s) 111, 392, 500, 908
ScaI AGTACT 1 cut(s) 1579
SchI GAGTC 1 cut(s) 417
ScrFI CCNGG 3 cut(s) 60, 483, 1404
SduI GDGCHC 2 cut(s) 602, 1023
SexAI ACCWGGT 1 cut(s) 481
SfaNI GCATC 4 cut(s) 188, 581, 1215, 1273
SfcI CTRYAG 2 cut(s) 1590, 1637
SinI GGWCC 3 cut(s) 111, 500, 908
SpeI ACTAGT 1 cut(s) 383
Sse9I AATT 7 cut(s) 15, 358, 514, 830, 937, 1000, 1260
SsiI CCGC 1 cut(s) 1527
SspMI CTAG 4 cut(s) 384, 614, 1565, 1575
SstI GAGCTC 1 cut(s) 602
StyD4I CCNGG 3 cut(s) 58, 481, 1402
StyI CCWWGG 1 cut(s) 100
TaaI ACNGT 4 cut(s) 42, 1332, 1582, 1672
TaqI TCGA 3 cut(s) 9, 1063, 1463
TasI AATT 7 cut(s) 15, 358, 514, 830, 937, 1000, 1260
TatI WGTACW 3 cut(s) 37, 687, 1577
TfiI GAWTC 2 cut(s) 239, 1151
Tru1I TTAA 4 cut(s) 14, 555, 1046, 1719
Tru9I TTAA 4 cut(s) 14, 555, 1046, 1719
TscAI CASTG 2 cut(s) 472, 1335
TseFI GTSAC 1 cut(s) 1432
Tsp45I GTSAC 1 cut(s) 1432
TspDTI ATGAA 8 cut(s) 182, 252, 260, 279, 297, 362, 660, 1143
TspRI CASTG 2 cut(s) 472, 1335
VpaK11BI GGWCC 3 cut(s) 111, 500, 908
VspI ATTAAT 1 cut(s) 1719
XapI RAATTY 2 cut(s) 830, 1260
XbaI TCTAGA 1 cut(s) 613
XceI RCATGY 1 cut(s) 770
XmnI GAANNNNTTC 1 cut(s) 651
XspI CTAG 4 cut(s) 384, 614, 1565, 1575
ZrmI AGTACT 1 cut(s) 1579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.