MD09G1060900.v1.1

3-phosphatase and dual-specificity protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0001990
Physical Location & Seq
Reverse (-)
4157991 .. 4160716
2726 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1060900.v1.1.491

Sequence Viewer

Length: 1242 bp
ATGGGGTTGAAGTTTTCAAGGCAAGTGCCGGCTGGGAAGTTTGAAAGCTTGACCATACAACACAAAGTGATCAATTGTCTGAGTAAAAGTCTTTACATCCGTAACTTGGTGTCGAAGCAACGGAGGCGAATGCTTGTTGCTGGTTATGATCTCGACATGACTTACATTACGGATCGCATTTTGGCAATGTCATTCCCTTCAGAACGAATGCGGGCAATGTATCGTAATCCTCTTTGGCAGGTCAAGTCTGTGCTTGATATGAGACATCAAGATCATTACAAGATCTATAACCTTTGCATCGAAGAAAGTTATGACCCGTCTCATTTTCATGGTCGTGTGGAGAGGTTTCCTTTTGATGACAATCATGTTCCACATCTAGAAATGATCAAGCTCTTCTGTGAAAATGTTGATTCATGGCTATCACAGGATCCGAAAAACATTGCCGTTATACACTGCATGGTAGGCAAAGGTAGAACGGGTTTAATGGTATGTGCTTACCTAGTCTACTGTGGGATGCCAGCAGAGGAAGCTCTTCTGTTGTATGCTCAAAGGCGGACCATCAATAATGAAGGAGTCTCAATACCAAGTCAACGCCGTTATGTAGGATACTGGTCGAACTGTCTCTCTTTACCTCACGGAGTTGAAGATGCACCACCTGATGTGAACTTGCCTCAGCAATGTAGCAGAGAATTGAGGAGAATCCGATTTTATGACACGGTTAACATCGAGAAAATCTTTTTTGTGGTGTCGGAGCTGCAAGAGATTTCCGGCCAGGTTTATCGTCTGTCACTTGAAGTTTCTAGGAGTTCCTGCAAACAAATCCAGAAAGGATGTCAGAGGACGAGTAGTCCACGATACTATCTCTCATTCGTCGAAGGCGATGAGGAAGATAAGAAATCAGAATCGGATGAGCCTCATGTTGTAGTCCAAATGGATACAGAAAGCTCGGCACTTTACCAGAAGGCCTGTCTTGAATATTACTTCGATAAGCCTGTGCAGGTAACTGGAGATGTGCGAGTCATTTTCTATCAAAAAATGTATGGAAGTCGCCTCTTCTATGCTTGCTTCAACACGGCTTTCATCAGGAACAGCTTGCTACAGCTCACTGTTCGGGATTTGGATAAAGTTGGAAAAAAAGGAAGATCGGTATGCGGCCCTGCCTTCTGCTTAGAGCTACTATTTGGTCCTGTCAATGCAAGACCCTCATTCACAACTCCATTGGATCATCATGAATTGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000278 GO:0000281 GO:0000910 GO:0001931 GO:0001932 GO:0001933 GO:0003674 GO:0003824 GO:0004721 GO:0004725 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005938 GO:0006464 GO:0006469 GO:0006470 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006807 GO:0006928 GO:0006935 GO:0006950 GO:0006996 GO:0007010 GO:0007015 GO:0007049 GO:0007154 GO:0007163 GO:0007165 GO:0007186 GO:0007187 GO:0007188 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0008154 GO:0009267 GO:0009555 GO:0009605 GO:0009892 GO:0009987 GO:0009991 GO:0010563 GO:0010605 GO:0014065 GO:0016020 GO:0016043 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019220 GO:0019222 GO:0019538 GO:0019637 GO:0022402 GO:0022603 GO:0022604 GO:0022607 GO:0023052 GO:0030010 GO:0030029 GO:0030030 GO:0030031 GO:0030036 GO:0030041 GO:0030258 GO:0030587 GO:0031152 GO:0031254 GO:0031257 GO:0031268 GO:0031269 GO:0031272 GO:0031279 GO:0031323 GO:0031324 GO:0031344 GO:0031399 GO:0031400 GO:0031667 GO:0031668 GO:0031669 GO:0032153 GO:0032155 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032796 GO:0032879 GO:0033036 GO:0033554 GO:0033673 GO:0034461 GO:0034485 GO:0034593 GO:0034594 GO:0034595 GO:0034613 GO:0034622 GO:0035335 GO:0035556 GO:0036051 GO:0036052 GO:0036211 GO:0040011 GO:0042221 GO:0042325 GO:0042326 GO:0042330 GO:0042578 GO:0042594 GO:0042995 GO:0043086 GO:0043170 GO:0043327 GO:0043412 GO:0043549 GO:0043933 GO:0044085 GO:0044087 GO:0044092 GO:0044237 GO:0044238 GO:0044255 GO:0044260 GO:0044267 GO:0044424 GO:0044425 GO:0044444 GO:0044448 GO:0044459 GO:0044464 GO:0044764 GO:0045761 GO:0045859 GO:0045936 GO:0046486 GO:0046488 GO:0046839 GO:0046856 GO:0048015 GO:0048017 GO:0048229 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048856 GO:0048870 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0050919 GO:0051128 GO:0051171 GO:0051172 GO:0051174 GO:0051179 GO:0051246 GO:0051248 GO:0051258 GO:0051270 GO:0051272 GO:0051285 GO:0051286 GO:0051301 GO:0051338 GO:0051339 GO:0051348 GO:0051641 GO:0051674 GO:0051703 GO:0051704 GO:0051716 GO:0051800 GO:0052866 GO:0060255 GO:0060491 GO:0061640 GO:0065003 GO:0065007 GO:0065009 GO:0070727 GO:0071496 GO:0071704 GO:0071840 GO:0071900 GO:0071901 GO:0071944 GO:0080090 GO:0090702 GO:0097435 GO:0098630 GO:0098743 GO:0099120 GO:0099568 GO:0099738 GO:0106017 GO:0120025 GO:0120031 GO:0120032 GO:0120035 GO:0120036 GO:0140096 GO:1901564 GO:1903047 GO:1990753
KEGG Pathways
Metabolic & Signaling
ko00562 ko01521 ko04068 ko04070 ko04071 ko04115 ko04140 ko04150 ko04151 ko04212 ko04218 ko04510 ko04931 ko05161 ko05165 ko05200 ko05206 ko05213 ko05214 ko05215 ko05218 ko05222 ko05224 ko05225 ko05230 map00562 map01521 map04068 map04070 map04071 map04115 map04140 map04150 map04151 map04212 map04218 map04510 map04931 map05161 map05165 map05200 map05206 map05213 map05214 map05215 map05218 map05222 map05224 map05225 map05230
Pfam Domains
Protein Families
DSPc PTEN_C2 Y_phosphatase3

Protein Analysis

414

Amino Acids

47.87

Weight (kDa)

8.43

Isoelectric Point (pI)

60.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTP-NADK PF22741 81 - 186 8.5e-07 DSP-PTPase phosphatase fused to NAD+ Kinase
PTP-SAK PF22784 112 - 178 5e-08 Swiss Army Knife protein, DSP-PTPase phosphatase domain
Tc-R-P PF22785 113 - 171 3.6e-09 Polymorphic toxin system, DSP-PTPase phosphatase
PTEN_C2 PF10409 312 - 395 2e-12 C2 domain of PTEN tumour-suppressor protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 229, 988
AccI GTMKAC 1 cut(s) 504
AciI CCGC 3 cut(s) 211, 553, 1152
AclWI GGATC 4 cut(s) 180, 422, 435, 1230
AcoI YGGCCR 1 cut(s) 769
AcuI CTGAAG 1 cut(s) 183
AdeI CACNNNGTG 1 cut(s) 67
AfiI CCNNNNNNNGG 1 cut(s) 106
AgsI TTSAA 7 cut(s) 10, 18, 44, 644, 794, 974, 1069
AhdI GACNNNNNGTC 1 cut(s) 846
AjnI CCWGG 1 cut(s) 771
AluBI AGCT 8 cut(s) 48, 391, 530, 754, 945, 1092, 1102, 1174
AluI AGCT 8 cut(s) 48, 391, 530, 754, 945, 1092, 1102, 1174
Alw26I GTCTC 4 cut(s) 256, 324, 580, 626
AlwI GGATC 4 cut(s) 180, 422, 435, 1230
AoxI GGCC 3 cut(s) 769, 963, 1153
ApeKI GCWGC 1 cut(s) 754
Asp700I GAANNNNTTC 1 cut(s) 531
AspS9I GGNCC 3 cut(s) 555, 1154, 1184
AvaII GGWCC 2 cut(s) 555, 1184
BamHI GGATCC 1 cut(s) 427
BbvCI CCTCAGC 1 cut(s) 672
BbvI GCAGC 1 cut(s) 741
BccI CCATC 1 cut(s) 566
BceAI ACGGC 3 cut(s) 428, 579, 1089
BciT130I CCWGG 1 cut(s) 773
BciVI GTATCC 2 cut(s) 599, 928
BclI TGATCA 2 cut(s) 69, 384
BcoDI GTCTC 4 cut(s) 256, 324, 580, 626
BfaI CTAG 3 cut(s) 377, 500, 801
BfmI CTRYAG 1 cut(s) 1097
BfuAI ACCTGC 2 cut(s) 229, 988
BfuI GTATCC 2 cut(s) 599, 928
BglII AGATCT 1 cut(s) 282
BisI GCNGC 2 cut(s) 755, 1153
BlsI GCNGC 2 cut(s) 756, 1154
Bme1390I CCNGG 1 cut(s) 773
Bme18I GGWCC 2 cut(s) 555, 1184
BmeRI GACNNNNNGTC 1 cut(s) 846
BmgT120I GGNCC 3 cut(s) 555, 1154, 1184
BmiI GGNNCC 1 cut(s) 429
BmrFI CCNGG 1 cut(s) 773
BmsI GCATC 3 cut(s) 306, 504, 637
BpmI CTGGAG 1 cut(s) 1026
Bpu10I CCTNAGC 1 cut(s) 672
BsaBI GATNNNNATC 1 cut(s) 360
Bsc4I CCNNNNNNNGG 1 cut(s) 106
Bse118I RCCGGY 1 cut(s) 28
Bse1I ACTGG 2 cut(s) 614, 1009
Bse3DI GCAATG 4 cut(s) 192, 222, 438, 683
Bse8I GATNNNNATC 1 cut(s) 360
BseBI CCWGG 1 cut(s) 773
BseGI GGATG 4 cut(s) 96, 519, 836, 913
BseJI GATNNNNATC 1 cut(s) 360
BseLI CCNNNNNNNGG 1 cut(s) 106
BseMI GCAATG 4 cut(s) 192, 222, 438, 683
BseMII CTCAG 2 cut(s) 71, 686
BseNI ACTGG 2 cut(s) 614, 1009
BseRI GAGGAG 1 cut(s) 709
BseXI GCAGC 1 cut(s) 741
BseYI CCCAGC 1 cut(s) 32
BsgI GTGCAG 1 cut(s) 1016
BshFI GGCC 3 cut(s) 771, 965, 1155
BsiSI CCGG 2 cut(s) 29, 768
BslI CCNNNNNNNGG 1 cut(s) 106
BsmAI GTCTC 4 cut(s) 256, 324, 580, 626
BsmBI CGTCTC 1 cut(s) 324
BsmI GAATGC 2 cut(s) 135, 213
BsnI GGCC 3 cut(s) 771, 965, 1155
Bsp143I GATC 9 cut(s) 69, 148, 172, 271, 282, 384, 427, 1142, 1222
BspACI CCGC 3 cut(s) 211, 553, 1152
BspANI GGCC 3 cut(s) 771, 965, 1155
BspCNI CTCAG 2 cut(s) 72, 685
BspHI TCATGA 1 cut(s) 1228
BspLI GGNNCC 1 cut(s) 429
BspMI ACCTGC 2 cut(s) 229, 988
BspPI GGATC 4 cut(s) 180, 422, 435, 1230
BspQI GCTCTTC 2 cut(s) 398, 537
BsrDI GCAATG 4 cut(s) 192, 222, 438, 683
BsrFI RCCGGY 1 cut(s) 28
BsrI ACTGG 2 cut(s) 614, 1009
BssAI RCCGGY 1 cut(s) 28
BssMI GATC 9 cut(s) 69, 148, 172, 271, 282, 384, 427, 1142, 1222
Bst2UI CCWGG 1 cut(s) 773
Bst4CI ACNGT 4 cut(s) 509, 620, 718, 1108
Bst6I CTCTTC 3 cut(s) 398, 537, 1058
BstC8I GCNNGC 5 cut(s) 30, 213, 519, 1063, 1094
BstDEI CTNAG 3 cut(s) 80, 672, 1168
BstF5I GGATG 4 cut(s) 96, 519, 836, 913
BstKTI GATC 9 cut(s) 72, 151, 175, 274, 285, 387, 430, 1145, 1225
BstMAI GTCTC 4 cut(s) 256, 324, 580, 626
BstMBI GATC 9 cut(s) 69, 148, 172, 271, 282, 384, 427, 1142, 1222
BstMWI GCNNNNNNNGC 3 cut(s) 124, 462, 527
BstNI CCWGG 1 cut(s) 773
BstSCI CCNGG 1 cut(s) 771
BstSFI CTRYAG 1 cut(s) 1097
BstV1I GCAGC 1 cut(s) 741
BstX2I RGATCY 2 cut(s) 282, 427
BstYI RGATCY 2 cut(s) 282, 427
BsuI GTATCC 2 cut(s) 599, 928
BsuRI GGCC 3 cut(s) 771, 965, 1155
BtgZI GCGATG 1 cut(s) 894
BtsCI GGATG 4 cut(s) 96, 519, 836, 913
BtsI GCAGTG 1 cut(s) 451
BtsIMutI CAGTG 2 cut(s) 451, 1104
BveI ACCTGC 2 cut(s) 229, 988
Cac8I GCNNGC 5 cut(s) 30, 213, 519, 1063, 1094
CciI TCATGA 1 cut(s) 1228
Cfr10I RCCGGY 1 cut(s) 28
Cfr13I GGNCC 3 cut(s) 555, 1154, 1184
CviAII CATG 7 cut(s) 157, 329, 365, 414, 457, 917, 1229
DdeI CTNAG 3 cut(s) 80, 672, 1168
DpnI GATC 9 cut(s) 71, 150, 174, 273, 284, 386, 429, 1144, 1224
DpnII GATC 9 cut(s) 69, 148, 172, 271, 282, 384, 427, 1142, 1222
DraIII CACNNNGTG 1 cut(s) 67
DriI GACNNNNNGTC 1 cut(s) 846
EaeI YGGCCR 1 cut(s) 769
Eam1104I CTCTTC 3 cut(s) 398, 537, 1058
Eam1105I GACNNNNNGTC 1 cut(s) 846
EarI CTCTTC 3 cut(s) 398, 537, 1058
EciI GGCGGA 1 cut(s) 568
Eco147I AGGCCT 1 cut(s) 965
Eco47I GGWCC 2 cut(s) 555, 1184
Eco57I CTGAAG 1 cut(s) 183
EcoRII CCWGG 1 cut(s) 771
Esp3I CGTCTC 1 cut(s) 324
FaeI CATG 7 cut(s) 160, 332, 368, 417, 460, 920, 1232
FatI CATG 7 cut(s) 156, 328, 364, 413, 456, 916, 1228
FauI CCCGC 1 cut(s) 204
FbaI TGATCA 2 cut(s) 69, 384
FblI GTMKAC 1 cut(s) 504
Fnu4HI GCNGC 2 cut(s) 755, 1153
FokI GGATG 4 cut(s) 83, 526, 843, 920
Fsp4HI GCNGC 2 cut(s) 755, 1153
FspBI CTAG 3 cut(s) 377, 500, 801
GluI GCNGC 2 cut(s) 755, 1153
GsaI CCCAGC 1 cut(s) 36
GsuI CTGGAG 1 cut(s) 1026
HaeIII GGCC 3 cut(s) 771, 965, 1155
HapII CCGG 2 cut(s) 29, 768
Hin1II CATG 7 cut(s) 160, 332, 368, 417, 460, 920, 1232
HincII GTYRAC 2 cut(s) 590, 721
HindII GTYRAC 2 cut(s) 590, 721
HindIII AAGCTT 1 cut(s) 46
HinfI GANTC 5 cut(s) 410, 573, 699, 902, 1017
HpaI GTTAAC 1 cut(s) 721
HpaII CCGG 2 cut(s) 29, 768
Hpy166II GTNNAC 5 cut(s) 505, 590, 664, 721, 851
Hpy188I TCNGA 8 cut(s) 81, 202, 432, 704, 751, 837, 901, 907
Hpy188III TCNNGA 9 cut(s) 152, 269, 377, 727, 823, 971, 1084, 1112, 1229
Hpy8I GTNNAC 5 cut(s) 505, 590, 664, 721, 851
Hpy99I CGWCG 1 cut(s) 875
HpyAV CCTTC 5 cut(s) 207, 563, 869, 955, 1171
HpyCH4III ACNGT 4 cut(s) 509, 620, 718, 1108
HpyCH4V TGCA 7 cut(s) 297, 456, 650, 757, 813, 997, 1196
HpyF10VI GCNNNNNNNGC 3 cut(s) 124, 462, 527
HpyF3I CTNAG 3 cut(s) 80, 672, 1168
Hsp92II CATG 7 cut(s) 160, 332, 368, 417, 460, 920, 1232
KroI GCCGGC 1 cut(s) 28
KroNI GCCGGC 1 cut(s) 30
Ksp22I TGATCA 2 cut(s) 69, 384
KspAI GTTAAC 1 cut(s) 721
Kzo9I GATC 9 cut(s) 69, 148, 172, 271, 282, 384, 427, 1142, 1222
LguI GCTCTTC 2 cut(s) 398, 537
LmnI GCTCC 1 cut(s) 751
Lsp1109I GCAGC 1 cut(s) 741
LweI GCATC 3 cut(s) 306, 504, 637
MaeI CTAG 3 cut(s) 377, 500, 801
MaeIII GTNAC 3 cut(s) 101, 786, 1000
MalI GATC 9 cut(s) 71, 150, 174, 273, 284, 386, 429, 1144, 1224
MboI GATC 9 cut(s) 69, 148, 172, 271, 282, 384, 427, 1142, 1222
MboII GAAGA 7 cut(s) 314, 385, 524, 656, 899, 1045, 1152
MfeI CAATTG 1 cut(s) 73
MflI RGATCY 2 cut(s) 282, 427
MluCI AATT 3 cut(s) 73, 689, 1232
MlyI GAGTC 2 cut(s) 582, 1026
MmeI TCCRAC 2 cut(s) 729, 1108
MroNI GCCGGC 1 cut(s) 28
MroXI GAANNNNTTC 1 cut(s) 531
MseI TTAA 2 cut(s) 482, 720
MslI CAYNNNNRTG 2 cut(s) 327, 333
MspI CCGG 2 cut(s) 29, 768
MspR9I CCNGG 1 cut(s) 773
MunI CAATTG 1 cut(s) 73
Mva1269I GAATGC 2 cut(s) 135, 213
MvaI CCWGG 1 cut(s) 773
MwoI GCNNNNNNNGC 3 cut(s) 124, 462, 527
NaeI GCCGGC 1 cut(s) 30
NdeII GATC 9 cut(s) 69, 148, 172, 271, 282, 384, 427, 1142, 1222
NgoMIV GCCGGC 1 cut(s) 28
NlaIII CATG 7 cut(s) 160, 332, 368, 417, 460, 920, 1232
NlaIV GGNNCC 1 cut(s) 429
NmeAIII GCCGAG 1 cut(s) 926
NmuCI GTSAC 1 cut(s) 786
PagI TCATGA 1 cut(s) 1228
PceI AGGCCT 1 cut(s) 965
PciSI GCTCTTC 2 cut(s) 398, 537
PcsI WCGNNNNNNNCGW 1 cut(s) 876
PctI GAATGC 2 cut(s) 135, 213
PdiI GCCGGC 1 cut(s) 30
PdmI GAANNNNTTC 1 cut(s) 531
PfeI GAWTC 3 cut(s) 410, 699, 902
PkrI GCNGC 2 cut(s) 756, 1154
PleI GAGTC 2 cut(s) 581, 1025
PpsI GAGTC 2 cut(s) 581, 1025
Psp6I CCWGG 1 cut(s) 771
PspFI CCCAGC 1 cut(s) 32
PspGI CCWGG 1 cut(s) 771
PspN4I GGNNCC 1 cut(s) 429
PspPI GGNCC 3 cut(s) 555, 1154, 1184
PsuI RGATCY 2 cut(s) 282, 427
RseI CAYNNNNRTG 2 cut(s) 327, 333
SapI GCTCTTC 2 cut(s) 398, 537
SaqAI TTAA 2 cut(s) 482, 720
SatI GCNGC 2 cut(s) 755, 1153
Sau3AI GATC 9 cut(s) 69, 148, 172, 271, 282, 384, 427, 1142, 1222
Sau96I GGNCC 3 cut(s) 555, 1154, 1184
SchI GAGTC 2 cut(s) 582, 1026
ScrFI CCNGG 1 cut(s) 773
SfaNI GCATC 3 cut(s) 306, 504, 637
SfcI CTRYAG 1 cut(s) 1097
SinI GGWCC 2 cut(s) 555, 1184
SmiMI CAYNNNNRTG 2 cut(s) 327, 333
Sse9I AATT 3 cut(s) 73, 689, 1232
SseBI AGGCCT 1 cut(s) 965
SsiI CCGC 3 cut(s) 211, 553, 1152
SspI AATATT 1 cut(s) 977
SspMI CTAG 3 cut(s) 377, 500, 801
StuI AGGCCT 1 cut(s) 965
StyD4I CCNGG 1 cut(s) 771
TaaI ACNGT 4 cut(s) 509, 620, 718, 1108
TaqI TCGA 7 cut(s) 113, 153, 300, 614, 726, 873, 984
TasI AATT 3 cut(s) 73, 689, 1232
TauI GCSGC 1 cut(s) 1155
TfiI GAWTC 3 cut(s) 410, 699, 902
Tru1I TTAA 2 cut(s) 482, 720
Tru9I TTAA 2 cut(s) 482, 720
TscAI CASTG 2 cut(s) 458, 1111
TseFI GTSAC 1 cut(s) 786
TseI GCWGC 1 cut(s) 754
Tsp45I GTSAC 1 cut(s) 786
TspDTI ATGAA 4 cut(s) 317, 402, 582, 1069
TspGWI ACGGA 4 cut(s) 89, 136, 185, 651
TspRI CASTG 2 cut(s) 458, 1111
VpaK11BI GGWCC 2 cut(s) 555, 1184
XbaI TCTAGA 1 cut(s) 376
XmiI GTMKAC 1 cut(s) 504
XmnI GAANNNNTTC 1 cut(s) 531
XspI CTAG 3 cut(s) 377, 500, 801
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.