MD17G1056300.v1.1

3-phosphatase and dual-specificity protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0001990
Physical Location & Seq
Reverse (-)
4445206 .. 4448133
2928 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1056300.v1.1.491

Sequence Viewer

Length: 1242 bp
ATGGGGTTGAAGTTCTCAAGGCAAGTGCCTGCAGGGAAGTTTGAAAGCTTAACCCTACAACACAAACTGATCAATTGTCTGAGTAAAAGCTTTTACATCCGTAACTTGGTGTCAAAGCAGCGGAGGCGAATGCTTGTTGCTGGCTATGATCTCGACATGACTTACATCACGGATCGCATTTTGGCAATGTCATTCCCTGCAGAACGAATGCGGGCAATGTATCGTAATCCTCTTTGGCAGGTCAAGTCTGTGCTTGATATGAGACATCAAGATCATTACAAGATCTACAACCTTTGCATCGAAGAAAATTATGACCCGTCTCATTTTCATGGTCGTGTGGAAAGGTTTCCTTTTGATGACAATCATGTTCCACATCTAGAAATGATCAAGCTCTTCTGTGAAAGTGTGGATTCATGGCTATCACAGGATCCGGAAAACATTGCCGTTATACACTGCATGGCAGGCAAAGGTCGCACGGGCTTAATGCTATGTGCTTACCTAGTCTACTGTGGGATGCCAGCAGAGGAAGCTCTTCAGTTGTATGCTCAAAGGCGGACCGTCAATAATGAAGGAGTCTCAATACCAAGCCAACGCCGTTATGTAGAATACTGGTCGAACTGTCTCTCTTTTCCTCGCGGAGTTGAAGATGTGCCTCCCGATGTGAACTTGCCTCAGCAATGTAGCAGAGAATTGAGACGAATCCGATTTTATGACACGATTAACATTGAAAAAATCTTTTTTGTGGTCTCAGAGTTGCAAGAGATTTCCGGCCAGCTTTATCGTCCATCACTAGAAGTTTCTAGGAGTTCCTGCAAACAAATCCAGAAAGGATGTCAGAGGACGAGTAGTCCACGATACTATGTCTCATTCGTTGAAGGCGAAGAGGAAGACAAGAAAGCAGAATCGGATGAGCCTCATGTTGTAGTCCAAATGGATACAGAAAGCTCCGTACTTTACCAGAAGGCCTGTCTTGAATATTACTTCGATATGCCTGTGCAGATAACTGGAGATGTGCGAGTCATATTCTATCAAAAAATGTATGGAAGTCGCCTCTTCTATGCTTGCTTCAACACAGCTTTCATCAGGAACAGCTTGCTACAGCTCACTGTACGGGATTTGGATAAAGTTGGGAAAAAAGGAGGATCGGTATGCGGCCCTGCCTTCTGCTTAGAGCTACTATTTGGTCCTGCGAATGCAAGACCCTCATTCACAAGTCCATCTGATCATCGTGAATTGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000278 GO:0000281 GO:0000910 GO:0001931 GO:0001932 GO:0001933 GO:0003674 GO:0003824 GO:0004721 GO:0004725 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005938 GO:0006464 GO:0006469 GO:0006470 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006807 GO:0006928 GO:0006935 GO:0006950 GO:0006996 GO:0007010 GO:0007015 GO:0007049 GO:0007154 GO:0007163 GO:0007165 GO:0007186 GO:0007187 GO:0007188 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0008154 GO:0009267 GO:0009555 GO:0009605 GO:0009892 GO:0009987 GO:0009991 GO:0010563 GO:0010605 GO:0014065 GO:0016020 GO:0016043 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019220 GO:0019222 GO:0019538 GO:0019637 GO:0022402 GO:0022603 GO:0022604 GO:0022607 GO:0023052 GO:0030010 GO:0030029 GO:0030030 GO:0030031 GO:0030036 GO:0030041 GO:0030258 GO:0030587 GO:0031152 GO:0031254 GO:0031257 GO:0031268 GO:0031269 GO:0031272 GO:0031279 GO:0031323 GO:0031324 GO:0031344 GO:0031399 GO:0031400 GO:0031667 GO:0031668 GO:0031669 GO:0032153 GO:0032155 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032796 GO:0032879 GO:0033036 GO:0033554 GO:0033673 GO:0034461 GO:0034485 GO:0034593 GO:0034594 GO:0034595 GO:0034613 GO:0034622 GO:0035335 GO:0035556 GO:0036051 GO:0036052 GO:0036211 GO:0040011 GO:0042221 GO:0042325 GO:0042326 GO:0042330 GO:0042578 GO:0042594 GO:0042995 GO:0043086 GO:0043170 GO:0043327 GO:0043412 GO:0043549 GO:0043933 GO:0044085 GO:0044087 GO:0044092 GO:0044237 GO:0044238 GO:0044255 GO:0044260 GO:0044267 GO:0044424 GO:0044425 GO:0044444 GO:0044448 GO:0044459 GO:0044464 GO:0044764 GO:0045761 GO:0045859 GO:0045936 GO:0046486 GO:0046488 GO:0046839 GO:0046856 GO:0048015 GO:0048017 GO:0048229 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048856 GO:0048870 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0050919 GO:0051128 GO:0051171 GO:0051172 GO:0051174 GO:0051179 GO:0051246 GO:0051248 GO:0051258 GO:0051270 GO:0051272 GO:0051285 GO:0051286 GO:0051301 GO:0051338 GO:0051339 GO:0051348 GO:0051641 GO:0051674 GO:0051703 GO:0051704 GO:0051716 GO:0051800 GO:0052866 GO:0060255 GO:0060491 GO:0061640 GO:0065003 GO:0065007 GO:0065009 GO:0070727 GO:0071496 GO:0071704 GO:0071840 GO:0071900 GO:0071901 GO:0071944 GO:0080090 GO:0090702 GO:0097435 GO:0098630 GO:0098743 GO:0099120 GO:0099568 GO:0099738 GO:0106017 GO:0120025 GO:0120031 GO:0120032 GO:0120035 GO:0120036 GO:0140096 GO:1901564 GO:1903047 GO:1990753
KEGG Pathways
Metabolic & Signaling
ko00562 ko01521 ko04068 ko04070 ko04071 ko04115 ko04140 ko04150 ko04151 ko04212 ko04218 ko04510 ko04931 ko05161 ko05165 ko05200 ko05206 ko05213 ko05214 ko05215 ko05218 ko05222 ko05224 ko05225 ko05230 map00562 map01521 map04068 map04070 map04071 map04115 map04140 map04150 map04151 map04212 map04218 map04510 map04931 map05161 map05165 map05200 map05206 map05213 map05214 map05215 map05218 map05222 map05224 map05225 map05230
Pfam Domains
Protein Families
DSPc PTEN_C2 Y_phosphatase3

Protein Analysis

414

Amino Acids

47.93

Weight (kDa)

7.87

Isoelectric Point (pI)

68.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTP-NADK PF22741 81 - 192 4.2e-07 DSP-PTPase phosphatase fused to NAD+ Kinase
PTP-SAK PF22784 101 - 178 3.6e-09 Swiss Army Knife protein, DSP-PTPase phosphatase domain
Tc-R-P PF22785 113 - 171 2.8e-10 Polymorphic toxin system, DSP-PTPase phosphatase
DSPc PF00782 117 - 188 8.6e-06 Dual specificity phosphatase, catalytic domain
PTEN_C2 PF10409 314 - 395 3.5e-11 C2 domain of PTEN tumour-suppressor protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 229
AccI GTMKAC 1 cut(s) 504
AccII CGCG 1 cut(s) 636
AccIII TCCGGA 1 cut(s) 430
AciI CCGC 5 cut(s) 121, 211, 553, 636, 1152
AclWI GGATC 4 cut(s) 180, 422, 435, 1150
AcoI YGGCCR 1 cut(s) 769
AcuI CTGAAG 1 cut(s) 518
AfaI GTAC 2 cut(s) 951, 1110
AfiI CCNNNNNNNGG 1 cut(s) 106
AgsI TTSAA 7 cut(s) 10, 44, 644, 728, 875, 974, 1069
AhdI GACNNNNNGTC 1 cut(s) 846
Alw26I GTCTC 7 cut(s) 256, 324, 580, 626, 688, 751, 868
AlwI GGATC 4 cut(s) 180, 422, 435, 1150
Aor13HI TCCGGA 1 cut(s) 430
AoxI GGCC 3 cut(s) 769, 963, 1153
ApeKI GCWGC 1 cut(s) 118
Asp700I GAANNNNTTC 2 cut(s) 345, 531
AspS9I GGNCC 3 cut(s) 555, 1154, 1184
AvaII GGWCC 2 cut(s) 555, 1184
BamHI GGATCC 1 cut(s) 427
BbsI GAAGAC 1 cut(s) 894
BbvCI CCTCAGC 1 cut(s) 672
BbvI GCAGC 1 cut(s) 130
BccI CCATC 2 cut(s) 793, 1225
BceAI ACGGC 2 cut(s) 428, 579
BciVI GTATCC 1 cut(s) 928
BclI TGATCA 3 cut(s) 69, 384, 1222
BcoDI GTCTC 7 cut(s) 256, 324, 580, 626, 688, 751, 868
BfaI CTAG 4 cut(s) 377, 500, 791, 801
BfmI CTRYAG 3 cut(s) 30, 198, 1097
BfuAI ACCTGC 1 cut(s) 229
BfuI GTATCC 1 cut(s) 928
BglII AGATCT 1 cut(s) 282
BisI GCNGC 2 cut(s) 119, 1153
BlsI GCNGC 2 cut(s) 120, 1154
Bme18I GGWCC 2 cut(s) 555, 1184
BmeRI GACNNNNNGTC 1 cut(s) 846
BmgT120I GGNCC 3 cut(s) 555, 1154, 1184
BmiI GGNNCC 1 cut(s) 429
BmsI GCATC 2 cut(s) 306, 504
BpiI GAAGAC 1 cut(s) 894
BpmI CTGGAG 1 cut(s) 1026
Bpu10I CCTNAGC 1 cut(s) 672
BsaBI GATNNNNATC 1 cut(s) 360
BsaI GGTCTC 1 cut(s) 751
BsaWI WCCGGW 1 cut(s) 430
BsaXI ACNNNNNCTCC 2 cut(s) 1131, 1161
Bsc4I CCNNNNNNNGG 1 cut(s) 106
Bse1I ACTGG 2 cut(s) 614, 1009
Bse3DI GCAATG 4 cut(s) 192, 222, 438, 683
Bse8I GATNNNNATC 1 cut(s) 360
BseAI TCCGGA 1 cut(s) 430
BseGI GGATG 4 cut(s) 96, 519, 836, 913
BseJI GATNNNNATC 1 cut(s) 360
BseLI CCNNNNNNNGG 1 cut(s) 106
BseMI GCAATG 4 cut(s) 192, 222, 438, 683
BseMII CTCAG 3 cut(s) 71, 686, 762
BseNI ACTGG 2 cut(s) 614, 1009
BseXI GCAGC 1 cut(s) 130
BsgI GTGCAG 1 cut(s) 1016
Bsh1236I CGCG 1 cut(s) 636
BshFI GGCC 3 cut(s) 771, 965, 1155
BsiSI CCGG 2 cut(s) 431, 768
BslI CCNNNNNNNGG 1 cut(s) 106
BsmAI GTCTC 7 cut(s) 256, 324, 580, 626, 688, 751, 868
BsmBI CGTCTC 2 cut(s) 324, 688
BsmI GAATGC 3 cut(s) 135, 213, 1198
BsnI GGCC 3 cut(s) 771, 965, 1155
Bso31I GGTCTC 1 cut(s) 751
Bsp13I TCCGGA 1 cut(s) 430
Bsp143I GATC 9 cut(s) 69, 148, 172, 271, 282, 384, 427, 1142, 1222
BspACI CCGC 5 cut(s) 121, 211, 553, 636, 1152
BspANI GGCC 3 cut(s) 771, 965, 1155
BspCNI CTCAG 3 cut(s) 72, 685, 761
BspEI TCCGGA 1 cut(s) 430
BspFNI CGCG 1 cut(s) 636
BspLI GGNNCC 1 cut(s) 429
BspMAI CTGCAG 2 cut(s) 34, 202
BspMI ACCTGC 1 cut(s) 229
BspPI GGATC 4 cut(s) 180, 422, 435, 1150
BspQI GCTCTTC 2 cut(s) 398, 537
BspTNI GGTCTC 1 cut(s) 751
BsrDI GCAATG 4 cut(s) 192, 222, 438, 683
BsrI ACTGG 2 cut(s) 614, 1009
BssMI GATC 9 cut(s) 69, 148, 172, 271, 282, 384, 427, 1142, 1222
Bst4CI ACNGT 4 cut(s) 509, 559, 620, 1108
Bst6I CTCTTC 4 cut(s) 398, 537, 876, 1058
BstC8I GCNNGC 8 cut(s) 30, 142, 213, 463, 519, 773, 1063, 1094
BstDEI CTNAG 4 cut(s) 80, 672, 748, 1168
BstF5I GGATG 4 cut(s) 96, 519, 836, 913
BstFNI CGCG 1 cut(s) 636
BstKTI GATC 9 cut(s) 72, 151, 175, 274, 285, 387, 430, 1145, 1225
BstMAI GTCTC 7 cut(s) 256, 324, 580, 626, 688, 751, 868
BstMBI GATC 9 cut(s) 69, 148, 172, 271, 282, 384, 427, 1142, 1222
BstMWI GCNNNNNNNGC 4 cut(s) 124, 462, 471, 527
BstSFI CTRYAG 3 cut(s) 30, 198, 1097
BstUI CGCG 1 cut(s) 636
BstV1I GCAGC 1 cut(s) 130
BstV2I GAAGAC 1 cut(s) 894
BstX2I RGATCY 2 cut(s) 282, 427
BstYI RGATCY 2 cut(s) 282, 427
BsuI GTATCC 1 cut(s) 928
BsuRI GGCC 3 cut(s) 771, 965, 1155
BtsCI GGATG 4 cut(s) 96, 519, 836, 913
BtsI GCAGTG 1 cut(s) 451
BtsIMutI CAGTG 2 cut(s) 451, 1104
BveI ACCTGC 1 cut(s) 229
Cac8I GCNNGC 8 cut(s) 30, 142, 213, 463, 519, 773, 1063, 1094
Cfr13I GGNCC 3 cut(s) 555, 1154, 1184
CpoI CGGWCCG 1 cut(s) 555
Csp6I GTAC 2 cut(s) 950, 1109
CspI CGGWCCG 1 cut(s) 555
CviAII CATG 6 cut(s) 157, 329, 365, 414, 457, 917
CviQI GTAC 2 cut(s) 950, 1109
DdeI CTNAG 4 cut(s) 80, 672, 748, 1168
DpnI GATC 9 cut(s) 71, 150, 174, 273, 284, 386, 429, 1144, 1224
DpnII GATC 9 cut(s) 69, 148, 172, 271, 282, 384, 427, 1142, 1222
DriI GACNNNNNGTC 1 cut(s) 846
EaeI YGGCCR 1 cut(s) 769
Eam1104I CTCTTC 4 cut(s) 398, 537, 876, 1058
Eam1105I GACNNNNNGTC 1 cut(s) 846
EarI CTCTTC 4 cut(s) 398, 537, 876, 1058
EciI GGCGGA 1 cut(s) 568
Eco147I AGGCCT 1 cut(s) 965
Eco31I GGTCTC 1 cut(s) 751
Eco47I GGWCC 2 cut(s) 555, 1184
Eco57I CTGAAG 1 cut(s) 518
Esp3I CGTCTC 2 cut(s) 324, 688
FaeI CATG 6 cut(s) 160, 332, 368, 417, 460, 920
FalI AAGNNNNNCTT 2 cut(s) 334, 366
FatI CATG 6 cut(s) 156, 328, 364, 413, 456, 916
FauI CCCGC 1 cut(s) 204
FbaI TGATCA 3 cut(s) 69, 384, 1222
FblI GTMKAC 1 cut(s) 504
Fnu4HI GCNGC 2 cut(s) 119, 1153
FokI GGATG 4 cut(s) 83, 526, 843, 920
Fsp4HI GCNGC 2 cut(s) 119, 1153
FspBI CTAG 4 cut(s) 377, 500, 791, 801
GluI GCNGC 2 cut(s) 119, 1153
GsuI CTGGAG 1 cut(s) 1026
HaeIII GGCC 3 cut(s) 771, 965, 1155
HapII CCGG 2 cut(s) 431, 768
Hin1II CATG 6 cut(s) 160, 332, 368, 417, 460, 920
HindIII AAGCTT 2 cut(s) 46, 88
HinfI GANTC 5 cut(s) 410, 573, 699, 902, 1017
HpaII CCGG 2 cut(s) 431, 768
Hpy166II GTNNAC 3 cut(s) 505, 664, 851
Hpy188I TCNGA 6 cut(s) 81, 704, 751, 837, 907, 1222
Hpy188III TCNNGA 9 cut(s) 152, 269, 377, 431, 656, 823, 971, 1084, 1229
Hpy8I GTNNAC 3 cut(s) 505, 664, 851
HpyAV CCTTC 4 cut(s) 563, 869, 955, 1171
HpyCH4III ACNGT 4 cut(s) 509, 559, 620, 1108
HpyCH4V TGCA 8 cut(s) 32, 200, 297, 456, 757, 813, 997, 1196
HpyF10VI GCNNNNNNNGC 4 cut(s) 124, 462, 471, 527
HpyF3I CTNAG 4 cut(s) 80, 672, 748, 1168
Hsp92II CATG 6 cut(s) 160, 332, 368, 417, 460, 920
Kpn2I TCCGGA 1 cut(s) 430
Ksp22I TGATCA 3 cut(s) 69, 384, 1222
Kzo9I GATC 9 cut(s) 69, 148, 172, 271, 282, 384, 427, 1142, 1222
LguI GCTCTTC 2 cut(s) 398, 537
LmnI GCTCC 1 cut(s) 950
Lsp1109I GCAGC 1 cut(s) 130
LweI GCATC 2 cut(s) 306, 504
MaeI CTAG 4 cut(s) 377, 500, 791, 801
MaeIII GTNAC 1 cut(s) 101
MalI GATC 9 cut(s) 71, 150, 174, 273, 284, 386, 429, 1144, 1224
MboI GATC 9 cut(s) 69, 148, 172, 271, 282, 384, 427, 1142, 1222
MboII GAAGA 7 cut(s) 314, 385, 524, 656, 893, 899, 1045
MfeI CAATTG 1 cut(s) 73
MflI RGATCY 2 cut(s) 282, 427
MluCI AATT 4 cut(s) 73, 307, 689, 1232
MlyI GAGTC 2 cut(s) 582, 1026
MroI TCCGGA 1 cut(s) 430
MroXI GAANNNNTTC 2 cut(s) 345, 531
MseI TTAA 3 cut(s) 50, 482, 720
MslI CAYNNNNRTG 2 cut(s) 327, 333
MspA1I CMGCKG 1 cut(s) 121
MspI CCGG 2 cut(s) 431, 768
MunI CAATTG 1 cut(s) 73
Mva1269I GAATGC 3 cut(s) 135, 213, 1198
MvnI CGCG 1 cut(s) 636
MwoI GCNNNNNNNGC 4 cut(s) 124, 462, 471, 527
NdeII GATC 9 cut(s) 69, 148, 172, 271, 282, 384, 427, 1142, 1222
NlaIII CATG 6 cut(s) 160, 332, 368, 417, 460, 920
NlaIV GGNNCC 1 cut(s) 429
PceI AGGCCT 1 cut(s) 965
PciSI GCTCTTC 2 cut(s) 398, 537
PcsI WCGNNNNNNNCGW 1 cut(s) 876
PctI GAATGC 3 cut(s) 135, 213, 1198
PdmI GAANNNNTTC 2 cut(s) 345, 531
PfeI GAWTC 3 cut(s) 410, 699, 902
PkrI GCNGC 2 cut(s) 120, 1154
PleI GAGTC 2 cut(s) 581, 1025
PpsI GAGTC 2 cut(s) 581, 1025
PspN4I GGNNCC 1 cut(s) 429
PspPI GGNCC 3 cut(s) 555, 1154, 1184
PstI CTGCAG 2 cut(s) 34, 202
PsuI RGATCY 2 cut(s) 282, 427
RsaI GTAC 2 cut(s) 951, 1110
RsaNI GTAC 2 cut(s) 950, 1109
RseI CAYNNNNRTG 2 cut(s) 327, 333
Rsr2I CGGWCCG 1 cut(s) 555
RsrII CGGWCCG 1 cut(s) 555
SapI GCTCTTC 2 cut(s) 398, 537
SaqAI TTAA 3 cut(s) 50, 482, 720
SatI GCNGC 2 cut(s) 119, 1153
Sau3AI GATC 9 cut(s) 69, 148, 172, 271, 282, 384, 427, 1142, 1222
Sau96I GGNCC 3 cut(s) 555, 1154, 1184
SbfI CCTGCAGG 1 cut(s) 34
SchI GAGTC 2 cut(s) 582, 1026
SdaI CCTGCAGG 1 cut(s) 34
SfaNI GCATC 2 cut(s) 306, 504
SfcI CTRYAG 3 cut(s) 30, 198, 1097
SinI GGWCC 2 cut(s) 555, 1184
SmiMI CAYNNNNRTG 2 cut(s) 327, 333
SmlI CTYRAG 1 cut(s) 16
SmoI CTYRAG 1 cut(s) 16
Sse8387I CCTGCAGG 1 cut(s) 34
Sse9I AATT 4 cut(s) 73, 307, 689, 1232
SseBI AGGCCT 1 cut(s) 965
SsiI CCGC 5 cut(s) 121, 211, 553, 636, 1152
SspI AATATT 1 cut(s) 977
SspMI CTAG 4 cut(s) 377, 500, 791, 801
StuI AGGCCT 1 cut(s) 965
TaaI ACNGT 4 cut(s) 509, 559, 620, 1108
TaqI TCGA 4 cut(s) 153, 300, 614, 984
TasI AATT 4 cut(s) 73, 307, 689, 1232
TauI GCSGC 1 cut(s) 1155
TfiI GAWTC 3 cut(s) 410, 699, 902
Tru1I TTAA 3 cut(s) 50, 482, 720
Tru9I TTAA 3 cut(s) 50, 482, 720
TscAI CASTG 2 cut(s) 458, 1111
TseI GCWGC 1 cut(s) 118
TspDTI ATGAA 4 cut(s) 317, 402, 582, 1069
TspGWI ACGGA 3 cut(s) 89, 185, 937
TspRI CASTG 2 cut(s) 458, 1111
VpaK11BI GGWCC 2 cut(s) 555, 1184
XbaI TCTAGA 1 cut(s) 376
XmiI GTMKAC 1 cut(s) 504
XmnI GAANNNNTTC 2 cut(s) 345, 531
XspI CTAG 4 cut(s) 377, 500, 791, 801
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.