Rorug06G0044600

3-phosphatase and dual-specificity protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001990
Physical Location & Seq
Forward (+)
5678909 .. 5682441
3533 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0044600.1

Sequence Viewer

Length: 1404 bp
ATGGCACCACTCACTATGTGTTTGAGTGTGCTTCTCTTATTGGCATTCTCAGGCATTGCCACTTCTCAGACAATCGTTGAGACTCTACCTGGGTGGAATGGGACACTTCCCTTCAAACTTGAAACAGGTTACATTGCGGTGGATGATTTGGATGAGGTGCAACTATTCTACTATTTCGTCGAGTCGGAGAGAAACCCAATTGATGATCCTCTTGTGCTTTGGCTTACTGGAGGCCCTGGATGTTCCGGGTTTTCTGCAATGGCTTACGAAATTGGTCCATTTAGTTTTGATCTGCATTACAATGGAAGCATACCCACTATAATTTTGAATCCATATTCATGGACCAAGGTGGCCAACATAATCTTTATAGATGCACCAGTTGGTGCTGGGTTTTCGTACTCTACAACCTCGGAAGGGTACTATACTTCCGATACAAAATCAGCTGCACAGACCTATACTTTCTTAAGAAAGTGGTTATATGCACACCCAAGTTTTCAGGGAAATCAACTGTACATTGGTGGTGACTCATATTCAGGACTTATTGTTCCCATTCTTCTTAAGAATATACTAGAAGGTTTAGAGTCTGGGTTAAGGCCAAGAATGCAACTCCAAGGATACTTGCTTGGAAATCCAGTTTCAGATTCTTTTATTGATGTAAATGAGAGAATCCCATATGTTCACCGAGTGTCACTCATTTCAGATGAAATCTATGAGGCTGCTAAAACTAATTGCAGTGGGGACTATGTCAATGTGGAACTTAACAATACACTTTGTGTTACTGCTCTTCAAAAAATTAAAGATTGTCTTCTACAAATAAATCTTGCACAAATTTTGGAACCCCAATGTGCTTTTGCATCTGGTAGAACAACTGAGTTGGAGTGGGATCTTAGAGCTCGAGAAGCAAGTACTATGGATTACCTCCTTTCAGAGTCTAAGCTACCTGAATTGCCTTGCCGGGGATATACATATGTGCTTGCCTATAGGTGGATGAACGATGAAACAGTTCGGGCAGCTCTAGGTGTTCGAAATGGAACAGTCGAGAATTGGGTACGATGCCCCAAGGCCTTGGCATACTACACAGAAGACATCACAAGTTCTCTTGCGTATCAGAAAAATCTATCAGAAACAGGTCTTCGAGCTTTGATATACAGTGGTGATCATGACATATCAGTTCCATGGATTGCTACACAATATTGGATCAAAGCTCTTGATATAGCTGTTTTTGATGAATGGCGACCGTGGTATGTCAACGGTCAAATTGCAGGATACCAAGTGAAGTTCATGAATGATAACTTCAGGTTGACTTACGTGACTGTAAAGGGTGCTGGTCACACTGCTCCAGAGTACAAACACAAGGAAACACTTGCCATGTTTGACAGATTTTTAGCACGTTACCCCATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000278 GO:0000281 GO:0000910 GO:0001931 GO:0001932 GO:0001933 GO:0003674 GO:0003824 GO:0004721 GO:0004725 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005938 GO:0006464 GO:0006469 GO:0006470 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006807 GO:0006928 GO:0006935 GO:0006950 GO:0006996 GO:0007010 GO:0007015 GO:0007049 GO:0007154 GO:0007163 GO:0007165 GO:0007186 GO:0007187 GO:0007188 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0008154 GO:0009267 GO:0009555 GO:0009605 GO:0009892 GO:0009987 GO:0009991 GO:0010563 GO:0010605 GO:0014065 GO:0016020 GO:0016043 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019220 GO:0019222 GO:0019538 GO:0019637 GO:0022402 GO:0022603 GO:0022604 GO:0022607 GO:0023052 GO:0030010 GO:0030029 GO:0030030 GO:0030031 GO:0030036 GO:0030041 GO:0030258 GO:0030587 GO:0031152 GO:0031254 GO:0031257 GO:0031268 GO:0031269 GO:0031272 GO:0031279 GO:0031323 GO:0031324 GO:0031344 GO:0031399 GO:0031400 GO:0031667 GO:0031668 GO:0031669 GO:0032153 GO:0032155 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032796 GO:0032879 GO:0033036 GO:0033554 GO:0033673 GO:0034461 GO:0034485 GO:0034593 GO:0034594 GO:0034595 GO:0034613 GO:0034622 GO:0035335 GO:0035556 GO:0036051 GO:0036052 GO:0036211 GO:0040011 GO:0042221 GO:0042325 GO:0042326 GO:0042330 GO:0042578 GO:0042594 GO:0042995 GO:0043086 GO:0043170 GO:0043327 GO:0043412 GO:0043549 GO:0043933 GO:0044085 GO:0044087 GO:0044092 GO:0044237 GO:0044238 GO:0044255 GO:0044260 GO:0044267 GO:0044424 GO:0044425 GO:0044444 GO:0044448 GO:0044459 GO:0044464 GO:0044764 GO:0045761 GO:0045859 GO:0045936 GO:0046486 GO:0046488 GO:0046839 GO:0046856 GO:0048015 GO:0048017 GO:0048229 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048856 GO:0048870 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0050919 GO:0051128 GO:0051171 GO:0051172 GO:0051174 GO:0051179 GO:0051246 GO:0051248 GO:0051258 GO:0051270 GO:0051272 GO:0051285 GO:0051286 GO:0051301 GO:0051338 GO:0051339 GO:0051348 GO:0051641 GO:0051674 GO:0051703 GO:0051704 GO:0051716 GO:0051800 GO:0052866 GO:0060255 GO:0060491 GO:0061640 GO:0065003 GO:0065007 GO:0065009 GO:0070727 GO:0071496 GO:0071704 GO:0071840 GO:0071900 GO:0071901 GO:0071944 GO:0080090 GO:0090702 GO:0097435 GO:0098630 GO:0098743 GO:0099120 GO:0099568 GO:0099738 GO:0106017 GO:0120025 GO:0120031 GO:0120032 GO:0120035 GO:0120036 GO:0140096 GO:1901564 GO:1903047 GO:1990753
KEGG Pathways
Metabolic & Signaling
ko00562 ko01521 ko04068 ko04070 ko04071 ko04115 ko04140 ko04150 ko04151 ko04212 ko04218 ko04510 ko04931 ko05161 ko05165 ko05200 ko05206 ko05213 ko05214 ko05215 ko05218 ko05222 ko05224 ko05225 ko05230 map00562 map01521 map04068 map04070 map04071 map04115 map04140 map04150 map04151 map04212 map04218 map04510 map04931 map05161 map05165 map05200 map05206 map05213 map05214 map05215 map05218 map05222 map05224 map05225 map05230
Pfam Domains
Protein Families
DSPc PTEN_C2 Y_phosphatase3

Protein Analysis

467

Amino Acids

52.56

Weight (kDa)

4.92

Isoelectric Point (pI)

28.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 30 - 463 2.6e-121 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 4
AciI CCGC 1 cut(s) 137
AclWI GGATC 3 cut(s) 200, 891, 1205
AcoI YGGCCR 1 cut(s) 351
AcsI RAATTY 1 cut(s) 828
AcuI CTGAAG 1 cut(s) 1279
AdeI CACNNNGTG 3 cut(s) 18, 685, 773
AfaI GTAC 6 cut(s) 398, 419, 512, 907, 1050, 1346
AfiI CCNNNNNNNGG 3 cut(s) 414, 956, 984
AflII CTTAAG 2 cut(s) 463, 557
AgsI TTSAA 4 cut(s) 115, 122, 328, 788
AjnI CCWGG 2 cut(s) 88, 235
AloI GAACNNNNNNTCC 2 cut(s) 528, 560
AluBI AGCT 7 cut(s) 443, 893, 937, 1013, 1139, 1205, 1217
AluI AGCT 7 cut(s) 443, 893, 937, 1013, 1139, 1205, 1217
Alw21I GWGCWC 1 cut(s) 895
Alw26I GTCTC 1 cut(s) 74
AlwI GGATC 3 cut(s) 200, 891, 1205
Ama87I CYCGRG 1 cut(s) 894
AoxI GGCC 4 cut(s) 232, 351, 593, 1062
ApeKI GCWGC 3 cut(s) 443, 716, 1010
ApoI RAATTY 1 cut(s) 828
Asp700I GAANNNNTTC 1 cut(s) 1002
AspS9I GGNCC 3 cut(s) 233, 275, 342
AsuC2I CCSGG 2 cut(s) 247, 956
AsuHPI GGTGA 3 cut(s) 533, 671, 1166
AsuII TTCGAA 1 cut(s) 1024
AvaI CYCGRG 1 cut(s) 894
AvaII GGWCC 2 cut(s) 275, 342
BalI TGGCCA 1 cut(s) 353
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 895
BbsI GAAGAC 3 cut(s) 797, 1089, 1124
Bbv12I GWGCWC 1 cut(s) 895
BbvI GCAGC 3 cut(s) 430, 703, 1022
BciT130I CCWGG 2 cut(s) 90, 237
BciVI GTATCC 2 cut(s) 608, 1259
BclI TGATCA 1 cut(s) 1156
BcnI CCSGG 2 cut(s) 247, 956
BcoDI GTCTC 1 cut(s) 74
BfaI CTAG 3 cut(s) 569, 1016, 1402
BfmI CTRYAG 1 cut(s) 979
BfrI CTTAAG 2 cut(s) 463, 557
BfuI GTATCC 2 cut(s) 608, 1259
BisI GCNGC 3 cut(s) 444, 717, 1011
BlsI GCNGC 3 cut(s) 445, 718, 1012
BmcAI AGTACT 1 cut(s) 907
Bme1390I CCNGG 4 cut(s) 90, 237, 247, 956
Bme18I GGWCC 2 cut(s) 275, 342
BmeT110I CYCGRG 1 cut(s) 894
BmgT120I GGNCC 3 cut(s) 233, 275, 342
BmiI GGNNCC 2 cut(s) 6, 837
BmrFI CCNGG 4 cut(s) 90, 237, 247, 956
BmsI GCATC 3 cut(s) 361, 863, 1043
BpiI GAAGAC 3 cut(s) 797, 1089, 1124
BplI GAGNNNNNCTC 2 cut(s) 675, 707
BpmI CTGGAG 2 cut(s) 249, 1323
Bpu14I TTCGAA 1 cut(s) 1024
BpuMI CCSGG 2 cut(s) 247, 956
BsaAI YACGTR 1 cut(s) 1309
Bsc4I CCNNNNNNNGG 3 cut(s) 414, 956, 984
Bse1I ACTGG 3 cut(s) 232, 377, 632
Bse3DI GCAATG 3 cut(s) 54, 132, 264
BseBI CCWGG 2 cut(s) 90, 237
BseGI GGATG 4 cut(s) 148, 157, 245, 993
BseLI CCNNNNNNNGG 3 cut(s) 414, 956, 984
BseMI GCAATG 3 cut(s) 54, 132, 264
BseMII CTCAG 3 cut(s) 63, 80, 861
BseNI ACTGG 3 cut(s) 232, 377, 632
BseXI GCAGC 3 cut(s) 430, 703, 1022
BseYI CCCAGC 1 cut(s) 386
BsgI GTGCAG 1 cut(s) 429
Bsh1285I CGRYCG 1 cut(s) 1238
BshFI GGCC 4 cut(s) 234, 353, 595, 1064
BshNI GGYRCC 1 cut(s) 4
BsiEI CGRYCG 1 cut(s) 1238
BsiHKAI GWGCWC 1 cut(s) 895
BsiHKCI CYCGRG 1 cut(s) 894
BsiSI CCGG 2 cut(s) 246, 955
BslFI GGGAC 2 cut(s) 115, 752
BslI CCNNNNNNNGG 3 cut(s) 414, 956, 984
BsmAI GTCTC 1 cut(s) 74
BsmFI GGGAC 2 cut(s) 115, 752
BsmI GAATGC 2 cut(s) 44, 606
BsnI GGCC 4 cut(s) 234, 353, 595, 1064
BsoBI CYCGRG 1 cut(s) 894
Bsp119I TTCGAA 1 cut(s) 1024
Bsp1286I GDGCHC 1 cut(s) 895
Bsp1407I TGTACA 1 cut(s) 510
Bsp143I GATC 5 cut(s) 205, 289, 883, 1156, 1197
Bsp19I CCATGG 1 cut(s) 1175
BspACI CCGC 1 cut(s) 137
BspANI GGCC 4 cut(s) 234, 353, 595, 1064
BspCNI CTCAG 3 cut(s) 62, 79, 862
BspHI TCATGA 2 cut(s) 1159, 1281
BspLI GGNNCC 2 cut(s) 6, 837
BspPI GGATC 3 cut(s) 200, 891, 1205
BspQI GCTCTTC 1 cut(s) 789
BspT104I TTCGAA 1 cut(s) 1024
BspT107I GGYRCC 1 cut(s) 4
BspTI CTTAAG 2 cut(s) 463, 557
BsrDI GCAATG 3 cut(s) 54, 132, 264
BsrGI TGTACA 1 cut(s) 510
BsrI ACTGG 3 cut(s) 232, 377, 632
BssMI GATC 5 cut(s) 205, 289, 883, 1156, 1197
BssT1I CCWWGG 5 cut(s) 345, 610, 1059, 1065, 1175
Bst2UI CCWGG 2 cut(s) 90, 237
Bst4CI ACNGT 7 cut(s) 510, 1003, 1036, 1151, 1239, 1253, 1315
Bst6I CTCTTC 1 cut(s) 789
BstAFI CTTAAG 2 cut(s) 463, 557
BstAUI TGTACA 1 cut(s) 510
BstBAI YACGTR 1 cut(s) 1309
BstBI TTCGAA 1 cut(s) 1024
BstC8I GCNNGC 1 cut(s) 975
BstDEI CTNAG 5 cut(s) 49, 66, 870, 887, 933
BstDSI CCRYGG 2 cut(s) 1175, 1238
BstF5I GGATG 4 cut(s) 148, 157, 245, 993
BstKTI GATC 5 cut(s) 208, 292, 886, 1159, 1200
BstMAI GTCTC 1 cut(s) 74
BstMBI GATC 5 cut(s) 205, 289, 883, 1156, 1197
BstMCI CGRYCG 1 cut(s) 1238
BstMWI GCNNNNNNNGC 2 cut(s) 601, 899
BstNI CCWGG 2 cut(s) 90, 237
BstSCI CCNGG 4 cut(s) 88, 235, 245, 954
BstSFI CTRYAG 1 cut(s) 979
BstV1I GCAGC 3 cut(s) 430, 703, 1022
BstV2I GAAGAC 3 cut(s) 797, 1089, 1124
BstX2I RGATCY 1 cut(s) 883
BstXI CCANNNNNNTGG 2 cut(s) 339, 1066
BstYI RGATCY 1 cut(s) 883
BsuI GTATCC 2 cut(s) 608, 1259
BsuRI GGCC 4 cut(s) 234, 353, 595, 1064
BtgI CCRYGG 2 cut(s) 1175, 1238
BtsCI GGATG 4 cut(s) 148, 157, 245, 993
BtsI GCAGTG 2 cut(s) 739, 1332
BtsIMutI CAGTG 3 cut(s) 739, 1156, 1332
Cac8I GCNNGC 1 cut(s) 975
CciI TCATGA 2 cut(s) 1159, 1281
Cfr13I GGNCC 3 cut(s) 233, 275, 342
Csp6I GTAC 6 cut(s) 397, 418, 511, 906, 1049, 1345
CviAII CATG 5 cut(s) 339, 1160, 1176, 1282, 1369
CviQI GTAC 6 cut(s) 397, 418, 511, 906, 1049, 1345
DdeI CTNAG 5 cut(s) 49, 66, 870, 887, 933
DpnI GATC 5 cut(s) 207, 291, 885, 1158, 1199
DpnII GATC 5 cut(s) 205, 289, 883, 1156, 1197
DraIII CACNNNGTG 3 cut(s) 18, 685, 773
EaeI YGGCCR 1 cut(s) 351
Eam1104I CTCTTC 1 cut(s) 789
EarI CTCTTC 1 cut(s) 789
Ecl136II GAGCTC 1 cut(s) 893
Eco130I CCWWGG 5 cut(s) 345, 610, 1059, 1065, 1175
Eco147I AGGCCT 1 cut(s) 1064
Eco24I GRGCYC 1 cut(s) 895
Eco47I GGWCC 2 cut(s) 275, 342
Eco53kI GAGCTC 1 cut(s) 893
Eco57I CTGAAG 1 cut(s) 1279
Eco88I CYCGRG 1 cut(s) 894
EcoICRI GAGCTC 1 cut(s) 893
EcoO109I RGGNCCY 1 cut(s) 233
EcoRII CCWGG 2 cut(s) 88, 235
EcoT14I CCWWGG 5 cut(s) 345, 610, 1059, 1065, 1175
EcoT38I GRGCYC 1 cut(s) 895
ErhI CCWWGG 5 cut(s) 345, 610, 1059, 1065, 1175
FaeI CATG 5 cut(s) 342, 1163, 1179, 1285, 1372
FalI AAGNNNNNCTT 2 cut(s) 789, 821
FaqI GGGAC 2 cut(s) 115, 752
FatI CATG 5 cut(s) 338, 1159, 1175, 1281, 1368
FauNDI CATATG 2 cut(s) 673, 967
FbaI TGATCA 1 cut(s) 1156
Fnu4HI GCNGC 3 cut(s) 444, 717, 1011
FokI GGATG 4 cut(s) 155, 164, 252, 1000
FriOI GRGCYC 1 cut(s) 895
Fsp4HI GCNGC 3 cut(s) 444, 717, 1011
FspBI CTAG 3 cut(s) 569, 1016, 1402
GluI GCNGC 3 cut(s) 444, 717, 1011
GsaI CCCAGC 1 cut(s) 390
GsuI CTGGAG 2 cut(s) 249, 1323
HaeIII GGCC 4 cut(s) 234, 353, 595, 1064
HapII CCGG 2 cut(s) 246, 955
Hin1II CATG 5 cut(s) 342, 1163, 1179, 1285, 1372
HincII GTYRAC 2 cut(s) 1249, 1302
HindII GTYRAC 2 cut(s) 1249, 1302
HinfI GANTC 8 cut(s) 82, 182, 328, 524, 581, 641, 666, 929
HpaII CCGG 2 cut(s) 246, 955
HphI GGTGA 3 cut(s) 533, 671, 1166
Hpy166II GTNNAC 3 cut(s) 679, 1249, 1302
Hpy188I TCNGA 9 cut(s) 69, 187, 412, 430, 640, 700, 928, 1110, 1123
Hpy188III TCNNGA 7 cut(s) 534, 896, 1039, 1160, 1208, 1282, 1340
Hpy8I GTNNAC 3 cut(s) 679, 1249, 1302
Hpy99I CGWCG 1 cut(s) 182
HpyAV CCTTC 3 cut(s) 121, 407, 566
HpyCH4III ACNGT 7 cut(s) 510, 1003, 1036, 1151, 1239, 1253, 1315
HpyCH4IV ACGT 2 cut(s) 1308, 1390
HpyF10VI GCNNNNNNNGC 2 cut(s) 601, 899
HpyF3I CTNAG 5 cut(s) 49, 66, 870, 887, 933
HpySE526I ACGT 2 cut(s) 1308, 1390
Hsp92II CATG 5 cut(s) 342, 1163, 1179, 1285, 1372
Ksp22I TGATCA 1 cut(s) 1156
Kzo9I GATC 5 cut(s) 205, 289, 883, 1156, 1197
LguI GCTCTTC 1 cut(s) 789
LmnI GCTCC 1 cut(s) 1342
Lsp1109I GCAGC 3 cut(s) 430, 703, 1022
LweI GCATC 3 cut(s) 361, 863, 1043
MaeI CTAG 3 cut(s) 569, 1016, 1402
MaeII ACGT 2 cut(s) 1308, 1390
MaeIII GTNAC 7 cut(s) 128, 521, 687, 775, 1309, 1328, 1391
MalI GATC 5 cut(s) 207, 291, 885, 1158, 1199
MboI GATC 5 cut(s) 205, 289, 883, 1156, 1197
MboII GAAGA 5 cut(s) 545, 776, 797, 1094, 1124
MfeI CAATTG 1 cut(s) 198
MflI RGATCY 1 cut(s) 883
MhlI GDGCHC 1 cut(s) 895
MlsI TGGCCA 1 cut(s) 353
MluCI AATT 9 cut(s) 198, 270, 321, 727, 792, 828, 944, 1042, 1257
MluNI TGGCCA 1 cut(s) 353
MlyI GAGTC 5 cut(s) 76, 191, 518, 590, 938
MmeI TCCRAC 2 cut(s) 165, 855
MnlI CCTC 6 cut(s) 148, 219, 224, 418, 706, 929
Mox20I TGGCCA 1 cut(s) 353
MroXI GAANNNNTTC 1 cut(s) 1002
MscI TGGCCA 1 cut(s) 353
MseI TTAA 5 cut(s) 464, 558, 590, 759, 795
MslI CAYNNNNRTG 3 cut(s) 137, 300, 337
Msp20I TGGCCA 1 cut(s) 353
MspA1I CMGCKG 1 cut(s) 443
MspCI CTTAAG 2 cut(s) 463, 557
MspI CCGG 2 cut(s) 246, 955
MspR9I CCNGG 4 cut(s) 90, 237, 247, 956
MunI CAATTG 1 cut(s) 198
Mva1269I GAATGC 2 cut(s) 44, 606
MvaI CCWGG 2 cut(s) 90, 237
MwoI GCNNNNNNNGC 2 cut(s) 601, 899
NciI CCSGG 2 cut(s) 247, 956
NcoI CCATGG 1 cut(s) 1175
NdeI CATATG 2 cut(s) 673, 967
NdeII GATC 5 cut(s) 205, 289, 883, 1156, 1197
NlaIII CATG 5 cut(s) 342, 1163, 1179, 1285, 1372
NlaIV GGNNCC 2 cut(s) 6, 837
NmuCI GTSAC 4 cut(s) 521, 687, 1309, 1328
NspV TTCGAA 1 cut(s) 1024
PaeR7I CTCGAG 1 cut(s) 894
PagI TCATGA 2 cut(s) 1159, 1281
PceI AGGCCT 1 cut(s) 1064
PciSI GCTCTTC 1 cut(s) 789
PctI GAATGC 2 cut(s) 44, 606
PdmI GAANNNNTTC 1 cut(s) 1002
PfeI GAWTC 3 cut(s) 328, 641, 666
PflFI GACNNNGTC 1 cut(s) 743
PkrI GCNGC 3 cut(s) 445, 718, 1012
PleI GAGTC 5 cut(s) 76, 190, 518, 589, 937
PpsI GAGTC 5 cut(s) 76, 190, 518, 589, 937
Ppu21I YACGTR 1 cut(s) 1309
Psp124BI GAGCTC 1 cut(s) 895
Psp6I CCWGG 2 cut(s) 88, 235
PspFI CCCAGC 1 cut(s) 386
PspGI CCWGG 2 cut(s) 88, 235
PspN4I GGNNCC 2 cut(s) 6, 837
PspPI GGNCC 3 cut(s) 233, 275, 342
PsuI RGATCY 1 cut(s) 883
PsyI GACNNNGTC 1 cut(s) 743
PvuII CAGCTG 1 cut(s) 443
RsaI GTAC 6 cut(s) 398, 419, 512, 907, 1050, 1346
RsaNI GTAC 6 cut(s) 397, 418, 511, 906, 1049, 1345
RseI CAYNNNNRTG 3 cut(s) 137, 300, 337
SacI GAGCTC 1 cut(s) 895
SapI GCTCTTC 1 cut(s) 789
SaqAI TTAA 5 cut(s) 464, 558, 590, 759, 795
SatI GCNGC 3 cut(s) 444, 717, 1011
Sau3AI GATC 5 cut(s) 205, 289, 883, 1156, 1197
Sau96I GGNCC 3 cut(s) 233, 275, 342
ScaI AGTACT 1 cut(s) 907
SchI GAGTC 5 cut(s) 76, 191, 518, 590, 938
ScrFI CCNGG 4 cut(s) 90, 237, 247, 956
SduI GDGCHC 1 cut(s) 895
SfaNI GCATC 3 cut(s) 361, 863, 1043
SfcI CTRYAG 1 cut(s) 979
Sfr274I CTCGAG 1 cut(s) 894
SfuI TTCGAA 1 cut(s) 1024
SinI GGWCC 2 cut(s) 275, 342
SlaI CTCGAG 1 cut(s) 894
SmiMI CAYNNNNRTG 3 cut(s) 137, 300, 337
SmlI CTYRAG 3 cut(s) 463, 557, 894
SmoI CTYRAG 3 cut(s) 463, 557, 894
Sse9I AATT 9 cut(s) 198, 270, 321, 727, 792, 828, 944, 1042, 1257
SseBI AGGCCT 1 cut(s) 1064
SsiI CCGC 1 cut(s) 137
SspI AATATT 1 cut(s) 1193
SspMI CTAG 3 cut(s) 569, 1016, 1402
SstI GAGCTC 1 cut(s) 895
StuI AGGCCT 1 cut(s) 1064
StyD4I CCNGG 4 cut(s) 88, 235, 245, 954
StyI CCWWGG 5 cut(s) 345, 610, 1059, 1065, 1175
TaaI ACNGT 7 cut(s) 510, 1003, 1036, 1151, 1239, 1253, 1315
TaiI ACGT 2 cut(s) 1311, 1393
TaqI TCGA 5 cut(s) 180, 895, 1024, 1038, 1135
TasI AATT 9 cut(s) 198, 270, 321, 727, 792, 828, 944, 1042, 1257
TatI WGTACW 3 cut(s) 510, 905, 1344
TfiI GAWTC 3 cut(s) 328, 641, 666
Tru1I TTAA 5 cut(s) 464, 558, 590, 759, 795
Tru9I TTAA 5 cut(s) 464, 558, 590, 759, 795
TscAI CASTG 3 cut(s) 739, 1156, 1339
TseFI GTSAC 4 cut(s) 521, 687, 1309, 1328
TseI GCWGC 3 cut(s) 443, 716, 1010
Tsp45I GTSAC 4 cut(s) 521, 687, 1309, 1328
TspDTI ATGAA 7 cut(s) 327, 717, 1004, 1011, 1242, 1270, 1298
TspRI CASTG 3 cut(s) 739, 1156, 1339
Tth111I GACNNNGTC 1 cut(s) 743
Vha464I CTTAAG 2 cut(s) 463, 557
VpaK11BI GGWCC 2 cut(s) 275, 342
XapI RAATTY 1 cut(s) 828
XhoI CTCGAG 1 cut(s) 894
XmnI GAANNNNTTC 1 cut(s) 1002
XspI CTAG 3 cut(s) 569, 1016, 1402
ZrmI AGTACT 1 cut(s) 907
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.