Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0001286

Physical Location & Seq

Genomic Coordinates Reverse (-)

31162424 .. 31169147

Gene Structure 6724 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1244000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1410 bp

ATGCCTTCACAGAAGCGTTCGTCACCAGAGCAGCACAGCGACGATGAAGACCATCGCCACCACCAACGACCCAAGCAATCTCGACCGCAAGATGAACACCAACTGCTGCAACAAGAAGAAGAAGAGGAAGAAGAAGAAGTAGCAGTAGAAGAAAAAGATGAAGACGACGAACACGATGCCGATTCTGATGCTAGCGCATCTTCTACTTCTCAAGAAACTCCCGAATTTATATTCATACAACTGCCAGAACTCCGTAAAGATGTACACTGTCCAATATGTCTTGGAATTATTAAGAAAACACGAACTGTAATGGGATGCCTACACAGGTTTTGCAGGGAATGTATCGACAAGTCAATGCGAATGGGGAACAATGAGTGTCCTGCTTGTCGCACACACTGTGCAAGTCGGCGTTCATTGAGAGATGATCCAAAGTATGATGCCCTAATAGCAGCTTTATATCCAGATATTGAGAAGTATGAGGAGGAGGAATTGGCTTTCCATCAGGAGGAGAGGACTCGGAACGAGCAGATCCAAGCATCAATTGCCAAAGTTTTTGAACAACAATCAGAAGCTCTAATCAAGAGACGCGCATCTGGAAAAGATACACCAGATTCGTTTCCTGTGAGACCGCAGCGCAATAGTCGGAGTTCTGTTTTAAGGAGACGGAACTCTCGAGCCACTGATATTCAAGGATATAAAGACAATGAGGATGATAATGACAATGACAATAATAACATGGGCAAAAATTTATCTTCCACTGATGACCGGCGTGAAGTTAGGCCAAGACGGCGGAGAAGACGGGCAGGATTTCGATCCTCTCAGCCTACTTCATCTCTGGCCAATTCAAGTGGAGGATGCACTGAAAATGATATAGAAGGGAAACGAGAGAACCACGGAATTTCACCTGGTGTTGTCTGGAGCTCTGAAATGCTTGTTTGGGGGCGGGGTGGTGCCCGGAGTCACACACGGCATGGCATTGCTAGTGGTTGCAACAGTAAGAATTCTCGGAGCACTCGCATTTCGAAGTTGGTTGATTATCTCCGGAGCTTAGAGGAAAACAATGATGAGTTAGATGTTCATCTCTTGCTTGTTCCTAAGGATACAAGTCGTACACCAAAGTTGCAGCAGCCACACCTTTGCTGTCGGCCGAGTTTGTCAGTGAAGCACCTTTGTGAATATGTTTCTCACCAGACGTCGTTGAGGGCTGAAGAAGTTGAATTATTAGAAGTGCCTCAGAGTTTCAATCACATTTCTGATGATGAGGAATTGGATCCGGATTCTGTGATAGATCCGATCAAAAATGACCTGAAAATTCTGCAAGAAGAAGAAACTTTGGCAGAGGTTAGAGCCAATAGCATTTCTTGCAGGGACTATTTGATTATAGCATACAGGCGGAAGGAGATTAGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0000152 GO:0001709 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010073 GO:0010074 GO:0010076 GO:0010077 GO:0010468 GO:0010492 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0019827 GO:0030154 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031519 GO:0032501 GO:0032502 GO:0032991 GO:0035102 GO:0040029 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045165 GO:0045814 GO:0045892 GO:0045934 GO:0048507 GO:0048519 GO:0048523 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0060255 GO:0065007 GO:0080090 GO:0098727 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1990234 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-C3HC4_2

Protein Analysis

470

Amino Acids

53.89

Weight (kDa)

5.55

Isoelectric Point (pI)

82.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	89 - 130	1e-07	Ring finger domain
zf-C3HC4_2	PF13923	90 - 129	6.1e-11	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	90 - 129	1.1e-09	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001286)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G44280 AT5G44280 AT5G44280
fragaria_vesca	FvH4_3g08840 FvH4_3g08840 FvH4_3g08840 FvH4_3g08840 FvH4_6g26540 FvH4_6g26540 FvH4_6g26540 FvH4_6g26540 FvH4_6g26540 FvH4_6g26540 FvH4_6g26540 FvH4_6g26540
malus_domestica	MD05G1312000.v1.1 MD09G1244000.v1.1 MD10G1291500.v1.1 MD17G1235900.v1.1
prunus_persica	Prupe.3G139900_v2.0.a1 Prupe.3G139900_v2.0.a1 Prupe.3G139900_v2.0.a1 Prupe.3G139900_v2.0.a1 Prupe.3G139900_v2.0.a1 Prupe.4G050700_v2.0.a1 Prupe.4G050700_v2.0.a1 Prupe.4G050700_v2.0.a1
pyrus_communis	pycom05g28800 pycom09g16250 pycom10g24370
rosa_chinensis	RchiOBHm_Chr2g0127611 RchiOBHm_Chr5g0008911
rosa_laevigata	RLG00000018950 RLG00000031615
rosa_multiflora	Rmu_co8349609.1_g000001 Rmu_sc0004677.1_g000011 Rmu_ssc0000200.1_g000019
rosa_roxburghii	Rroxscaffold_1G00067100 Rroxscaffold_1G00067720 Rroxscaffold_2G00116570
rosa_rugosa	Rorug02G0272500 Rorug02G0272500 Rorug02G0272500 Rorug02G0272500 Rorug04G0440100
rosa_samantha	Rh2AG324500 Rh2BG333800 Rh2CG312000 Rh2DG352000 Rh5AG068800 Rh5BG065500 Rh5CG076700 Rh5DG064500
rosa_wichuraiana	Rw2G026350 Rw5G006440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1196
AccB1I	GGYRCC	1 cut(s)	950
AccII	CGCG	1 cut(s)	588
AccIII	TCCGGA	2 cut(s)	1041, 1273
AciI	CCGC	5 cut(s)	86, 629, 790, 943, 1393
AclWI	GGATC	6 cut(s)	419, 523, 807, 1265, 1278, 1283
AcoI	YGGCCR	2 cut(s)	837, 1145
AcsI	RAATTY	5 cut(s)	224, 745, 897, 1000, 1311
AcuI	CTGAAG	1 cut(s)	1227
AcyI	GRCGYC	1 cut(s)	1193
AdeI	CACNNNGTG	2 cut(s)	398, 908
AfaI	GTAC	2 cut(s)	264, 1111
AfiI	CCNNNNNNNGG	1 cut(s)	505
AgsI	TTSAA	5 cut(s)	557, 689, 846, 1217, 1243
AjnI	CCWGG	1 cut(s)	904
AleI	CACNNNNGTG	1 cut(s)	1170
AluBI	AGCT	4 cut(s)	452, 572, 921, 1047
AluI	AGCT	4 cut(s)	452, 572, 921, 1047
Alw21I	GWGCWC	2 cut(s)	923, 1013
Alw26I	GTCTC	3 cut(s)	577, 619, 655
AlwI	GGATC	6 cut(s)	419, 523, 807, 1265, 1278, 1283
Ama87I	CYCGRG	1 cut(s)	672
Aor13HI	TCCGGA	2 cut(s)	1041, 1273
AoxI	GGCC	3 cut(s)	779, 837, 1145
ApeKI	GCWGC	6 cut(s)	31, 106, 449, 631, 1123, 1126
ApoI	RAATTY	5 cut(s)	224, 745, 897, 1000, 1311
AspLEI	GCGC	3 cut(s)	197, 590, 636
AsuC2I	CCSGG	1 cut(s)	955
AsuHPI	GGTGA	3 cut(s)	15, 894, 1178
AsuII	TTCGAA	1 cut(s)	1022
AsuNHI	GCTAGC	1 cut(s)	191
AvaI	CYCGRG	1 cut(s)	672
AxyI	CCTNAGG	1 cut(s)	1095
BaeGI	GKGCMC	1 cut(s)	955
BalI	TGGCCA	1 cut(s)	839
BamHI	GGATCC	1 cut(s)	1270
BanI	GGYRCC	1 cut(s)	950
BanII	GRGCYC	1 cut(s)	923
BbsI	GAAGAC	3 cut(s)	54, 168, 802
Bbv12I	GWGCWC	2 cut(s)	923, 1013
BbvI	GCAGC	6 cut(s)	43, 93, 461, 643, 1135, 1138
BccI	CCATC	2 cut(s)	60, 507
BceAI	ACGGC	2 cut(s)	803, 983
BcgI	CGANNNNNNTGC	4 cut(s)	158, 170, 192, 204
BciT130I	CCWGG	1 cut(s)	906
BciVI	GTATCC	1 cut(s)	1093
BcnI	CCSGG	1 cut(s)	955
BcoDI	GTCTC	3 cut(s)	577, 619, 655
BfaI	CTAG	2 cut(s)	192, 981
BfuI	GTATCC	1 cut(s)	1093
BglI	GCCNNNNNGGC	1 cut(s)	787
BisI	GCNGC	6 cut(s)	32, 107, 450, 632, 1124, 1127
BlsI	GCNGC	6 cut(s)	33, 108, 451, 633, 1125, 1128
Bme1390I	CCNGG	2 cut(s)	906, 955
BmeT110I	CYCGRG	1 cut(s)	672
BmiI	GGNNCC	2 cut(s)	952, 1272
BmrFI	CCNGG	2 cut(s)	906, 955
BmsI	GCATC	8 cut(s)	166, 178, 206, 305, 427, 545, 599, 845
BmtI	GCTAGC	1 cut(s)	195
BpiI	GAAGAC	3 cut(s)	54, 168, 802
BpmI	CTGGAG	1 cut(s)	937
Bpu14I	TTCGAA	1 cut(s)	1022
BpuEI	CTTGAG	1 cut(s)	195
BpuMI	CCSGG	1 cut(s)	955
BsaBI	GATNNNNATC	2 cut(s)	811, 1077
BsaHI	GRCGYC	1 cut(s)	1193
BsaI	GGTCTC	1 cut(s)	619
BsaJI	CCNNGG	1 cut(s)	892
BsaWI	WCCGGW	2 cut(s)	1041, 1273
Bsc4I	CCNNNNNNNGG	1 cut(s)	505
Bse118I	RCCGGY	1 cut(s)	765
Bse21I	CCTNAGG	1 cut(s)	1095
Bse3DI	GCAATG	1 cut(s)	975
Bse8I	GATNNNNATC	2 cut(s)	811, 1077
BseAI	TCCGGA	2 cut(s)	1041, 1273
BseBI	CCWGG	1 cut(s)	906
BseDI	CCNNGG	1 cut(s)	892
BseGI	GGATG	3 cut(s)	320, 715, 860
BseJI	GATNNNNATC	2 cut(s)	811, 1077
BseLI	CCNNNNNNNGG	1 cut(s)	505
BseMI	GCAATG	1 cut(s)	975
BseMII	CTCAG	2 cut(s)	833, 1247
BseRI	GAGGAG	3 cut(s)	494, 497, 521
BseSI	GKGCMC	1 cut(s)	955
BseX3I	CGGCCG	1 cut(s)	1145
BseXI	GCAGC	6 cut(s)	43, 93, 461, 643, 1135, 1138
Bsh1236I	CGCG	1 cut(s)	588
Bsh1285I	CGRYCG	2 cut(s)	86, 1148
BshFI	GGCC	3 cut(s)	781, 839, 1147
BshNI	GGYRCC	1 cut(s)	950
BsiEI	CGRYCG	2 cut(s)	86, 1148
BsiHKAI	GWGCWC	2 cut(s)	923, 1013
BsiHKCI	CYCGRG	1 cut(s)	672
BsiSI	CCGG	4 cut(s)	766, 955, 1042, 1274
BslFI	GGGAC	1 cut(s)	1382
BslI	CCNNNNNNNGG	1 cut(s)	505
BsmAI	GTCTC	3 cut(s)	577, 619, 655
BsmBI	CGTCTC	2 cut(s)	577, 655
BsmFI	GGGAC	1 cut(s)	1382
BsnI	GGCC	3 cut(s)	781, 839, 1147
Bso31I	GGTCTC	1 cut(s)	619
BsoBI	CYCGRG	1 cut(s)	672
Bsp119I	TTCGAA	1 cut(s)	1022
Bsp1286I	GDGCHC	3 cut(s)	923, 955, 1013
Bsp13I	TCCGGA	2 cut(s)	1041, 1273
Bsp1407I	TGTACA	1 cut(s)	262
Bsp143I	GATC	6 cut(s)	424, 528, 812, 1270, 1288, 1293
BspACI	CCGC	5 cut(s)	86, 629, 790, 943, 1393
BspANI	GGCC	3 cut(s)	781, 839, 1147
BspCNI	CTCAG	2 cut(s)	832, 1246
BspEI	TCCGGA	2 cut(s)	1041, 1273
BspFNI	CGCG	1 cut(s)	588
BspLI	GGNNCC	2 cut(s)	952, 1272
BspOI	GCTAGC	1 cut(s)	195
BspPI	GGATC	6 cut(s)	419, 523, 807, 1265, 1278, 1283
BspT104I	TTCGAA	1 cut(s)	1022
BspT107I	GGYRCC	1 cut(s)	950
BspTNI	GGTCTC	1 cut(s)	619
BsrDI	GCAATG	1 cut(s)	975
BsrFI	RCCGGY	1 cut(s)	765
BsrGI	TGTACA	1 cut(s)	262
BssAI	RCCGGY	1 cut(s)	765
BssECI	CCNNGG	1 cut(s)	892
BssMI	GATC	6 cut(s)	424, 528, 812, 1270, 1288, 1293
BssNI	GRCGYC	1 cut(s)	1193
Bst2UI	CCWGG	1 cut(s)	906
Bst4CI	ACNGT	4 cut(s)	269, 307, 398, 995
Bst6I	CTCTTC	1 cut(s)	117
BstACI	GRCGYC	1 cut(s)	1193
BstAPI	GCANNNNNTGC	2 cut(s)	542, 1362
BstAUI	TGTACA	1 cut(s)	262
BstBI	TTCGAA	1 cut(s)	1022
BstC8I	GCNNGC	1 cut(s)	193
BstDEI	CTNAG	4 cut(s)	819, 1048, 1095, 1233
BstDSI	CCRYGG	1 cut(s)	892
BstF5I	GGATG	3 cut(s)	320, 715, 860
BstFNI	CGCG	1 cut(s)	588
BstHHI	GCGC	3 cut(s)	197, 590, 636
BstKTI	GATC	6 cut(s)	427, 531, 815, 1273, 1291, 1296
BstMAI	GTCTC	3 cut(s)	577, 619, 655
BstMBI	GATC	6 cut(s)	424, 528, 812, 1270, 1288, 1293
BstMCI	CGRYCG	2 cut(s)	86, 1148
BstMWI	GCNNNNNNNGC	4 cut(s)	446, 542, 787, 1362
BstNI	CCWGG	1 cut(s)	906
BstSCI	CCNGG	2 cut(s)	904, 953
BstSLI	GKGCMC	1 cut(s)	955
BstUI	CGCG	1 cut(s)	588
BstV1I	GCAGC	6 cut(s)	43, 93, 461, 643, 1135, 1138
BstV2I	GAAGAC	3 cut(s)	54, 168, 802
BstX2I	RGATCY	3 cut(s)	528, 1270, 1288
BstYI	RGATCY	3 cut(s)	528, 1270, 1288
BstZI	CGGCCG	1 cut(s)	1145
Bsu36I	CCTNAGG	1 cut(s)	1095
BsuI	GTATCC	1 cut(s)	1093
BsuRI	GGCC	3 cut(s)	781, 839, 1147
BtgI	CCRYGG	1 cut(s)	892
BtgZI	GCGATG	1 cut(s)	38
BtsCI	GGATG	3 cut(s)	320, 715, 860
BtsIMutI	CAGTG	6 cut(s)	265, 394, 678, 756, 858, 1164
Cac8I	GCNNGC	1 cut(s)	193
CfoI	GCGC	3 cut(s)	197, 590, 636
Cfr10I	RCCGGY	1 cut(s)	765
CseI	GACGC	1 cut(s)	594
CsiI	ACCWGGT	1 cut(s)	904
Csp6I	GTAC	2 cut(s)	263, 1110
CspCI	CAANNNNNGTGG	2 cut(s)	829, 864
CviAII	CATG	2 cut(s)	736, 971
CviQI	GTAC	2 cut(s)	263, 1110
DdeI	CTNAG	4 cut(s)	819, 1048, 1095, 1233
DpnI	GATC	6 cut(s)	426, 530, 814, 1272, 1290, 1295
DpnII	GATC	6 cut(s)	424, 528, 812, 1270, 1288, 1293
DraIII	CACNNNGTG	2 cut(s)	398, 908
EaeI	YGGCCR	2 cut(s)	837, 1145
EagI	CGGCCG	1 cut(s)	1145
Eam1104I	CTCTTC	1 cut(s)	117
EarI	CTCTTC	1 cut(s)	117
EciI	GGCGGA	2 cut(s)	805, 1408
Ecl136II	GAGCTC	1 cut(s)	921
EclXI	CGGCCG	1 cut(s)	1145
Eco24I	GRGCYC	1 cut(s)	923
Eco31I	GGTCTC	1 cut(s)	619
Eco52I	CGGCCG	1 cut(s)	1145
Eco53kI	GAGCTC	1 cut(s)	921
Eco57I	CTGAAG	1 cut(s)	1227
Eco81I	CCTNAGG	1 cut(s)	1095
Eco88I	CYCGRG	1 cut(s)	672
EcoICRI	GAGCTC	1 cut(s)	921
EcoRI	GAATTC	1 cut(s)	1000
EcoRII	CCWGG	1 cut(s)	904
EcoT38I	GRGCYC	1 cut(s)	923
Esp3I	CGTCTC	2 cut(s)	577, 655
FaeI	CATG	2 cut(s)	739, 974
FaqI	GGGAC	1 cut(s)	1382
FatI	CATG	2 cut(s)	735, 970
FauI	CCCGC	1 cut(s)	936
Fnu4HI	GCNGC	6 cut(s)	32, 107, 450, 632, 1124, 1127
FokI	GGATG	3 cut(s)	327, 722, 867
FriOI	GRGCYC	1 cut(s)	923
Fsp4HI	GCNGC	6 cut(s)	32, 107, 450, 632, 1124, 1127
FspBI	CTAG	2 cut(s)	192, 981
GlaI	GCGC	3 cut(s)	196, 589, 635
GluI	GCNGC	6 cut(s)	32, 107, 450, 632, 1124, 1127
GsuI	CTGGAG	1 cut(s)	937
HaeIII	GGCC	3 cut(s)	781, 839, 1147
HapII	CCGG	4 cut(s)	766, 955, 1042, 1274
HgaI	GACGC	1 cut(s)	594
HhaI	GCGC	3 cut(s)	197, 590, 636
Hin1I	GRCGYC	1 cut(s)	1193
Hin1II	CATG	2 cut(s)	739, 974
Hin6I	GCGC	3 cut(s)	195, 588, 634
HinP1I	GCGC	3 cut(s)	195, 588, 634
HinfI	GANTC	5 cut(s)	182, 514, 611, 958, 1277
HpaII	CCGG	4 cut(s)	766, 955, 1042, 1274
HphI	GGTGA	3 cut(s)	15, 894, 1178
Hpy166II	GTNNAC	2 cut(s)	265, 1112
Hpy188I	TCNGA	9 cut(s)	187, 519, 568, 645, 925, 1008, 1236, 1255, 1293
Hpy8I	GTNNAC	2 cut(s)	265, 1112
Hpy99I	CGWCG	3 cut(s)	44, 170, 1198
HpyAV	CCTTC	3 cut(s)	15, 869, 1390
HpyCH4III	ACNGT	4 cut(s)	269, 307, 398, 995
HpyCH4IV	ACGT	1 cut(s)	1193
HpyCH4V	TGCA	8 cut(s)	109, 333, 401, 858, 990, 1123, 1318, 1365
HpyF10VI	GCNNNNNNNGC	4 cut(s)	446, 542, 787, 1362
HpyF3I	CTNAG	4 cut(s)	819, 1048, 1095, 1233
HpySE526I	ACGT	1 cut(s)	1193
Hsp92I	GRCGYC	1 cut(s)	1193
Hsp92II	CATG	2 cut(s)	739, 974
HspAI	GCGC	3 cut(s)	195, 588, 634
Kpn2I	TCCGGA	2 cut(s)	1041, 1273
Kzo9I	GATC	6 cut(s)	424, 528, 812, 1270, 1288, 1293
LmnI	GCTCC	3 cut(s)	918, 1008, 1044
Lsp1109I	GCAGC	6 cut(s)	43, 93, 461, 643, 1135, 1138
LweI	GCATC	8 cut(s)	166, 178, 206, 305, 427, 545, 599, 845
MabI	ACCWGGT	1 cut(s)	904
MaeI	CTAG	2 cut(s)	192, 981
MaeII	ACGT	1 cut(s)	1193
MaeIII	GTNAC	2 cut(s)	21, 959
MalI	GATC	6 cut(s)	426, 530, 814, 1272, 1290, 1295
MboI	GATC	6 cut(s)	424, 528, 812, 1270, 1288, 1293
MfeI	CAATTG	1 cut(s)	540
MflI	RGATCY	3 cut(s)	528, 1270, 1288
MhlI	GDGCHC	3 cut(s)	923, 955, 1013
MlsI	TGGCCA	1 cut(s)	839
MluNI	TGGCCA	1 cut(s)	839
MlyI	GAGTC	2 cut(s)	508, 967
MmeI	TCCRAC	1 cut(s)	623
Mox20I	TGGCCA	1 cut(s)	839
MroI	TCCGGA	2 cut(s)	1041, 1273
MscI	TGGCCA	1 cut(s)	839
MseI	TTAA	2 cut(s)	291, 656
MslI	CAYNNNNRTG	1 cut(s)	1170
Msp20I	TGGCCA	1 cut(s)	839
MspI	CCGG	4 cut(s)	766, 955, 1042, 1274
MspR9I	CCNGG	2 cut(s)	906, 955
MunI	CAATTG	1 cut(s)	540
MvaI	CCWGG	1 cut(s)	906
MvnI	CGCG	1 cut(s)	588
MwoI	GCNNNNNNNGC	4 cut(s)	446, 542, 787, 1362
NciI	CCSGG	1 cut(s)	955
NdeII	GATC	6 cut(s)	424, 528, 812, 1270, 1288, 1293
NheI	GCTAGC	1 cut(s)	191
NlaIII	CATG	2 cut(s)	739, 974
NlaIV	GGNNCC	2 cut(s)	952, 1272
NmeAIII	GCCGAG	1 cut(s)	1173
NmuCI	GTSAC	2 cut(s)	21, 959
NspV	TTCGAA	1 cut(s)	1022
OliI	CACNNNNGTG	1 cut(s)	1170
PaeR7I	CTCGAG	1 cut(s)	672
PcsI	WCGNNNNNNNCGW	2 cut(s)	171, 670
PfeI	GAWTC	3 cut(s)	182, 611, 1277
PkrI	GCNGC	6 cut(s)	33, 108, 451, 633, 1125, 1128
PleI	GAGTC	2 cut(s)	508, 966
PpsI	GAGTC	2 cut(s)	508, 966
Psp124BI	GAGCTC	1 cut(s)	923
Psp6I	CCWGG	1 cut(s)	904
PspGI	CCWGG	1 cut(s)	904
PspN4I	GGNNCC	2 cut(s)	952, 1272
PsuI	RGATCY	3 cut(s)	528, 1270, 1288
RsaI	GTAC	2 cut(s)	264, 1111
RsaNI	GTAC	2 cut(s)	263, 1110
RseI	CAYNNNNRTG	1 cut(s)	1170
SacI	GAGCTC	1 cut(s)	923
SaqAI	TTAA	2 cut(s)	291, 656
SatI	GCNGC	6 cut(s)	32, 107, 450, 632, 1124, 1127
Sau3AI	GATC	6 cut(s)	424, 528, 812, 1270, 1288, 1293
SchI	GAGTC	2 cut(s)	508, 967
ScrFI	CCNGG	2 cut(s)	906, 955
SduI	GDGCHC	3 cut(s)	923, 955, 1013
SexAI	ACCWGGT	1 cut(s)	904
SfaNI	GCATC	8 cut(s)	166, 178, 206, 305, 427, 545, 599, 845
Sfr274I	CTCGAG	1 cut(s)	672
SfuI	TTCGAA	1 cut(s)	1022
SlaI	CTCGAG	1 cut(s)	672
SmiMI	CAYNNNNRTG	1 cut(s)	1170
SmlI	CTYRAG	2 cut(s)	210, 672
SmoI	CTYRAG	2 cut(s)	210, 672
SsiI	CCGC	5 cut(s)	86, 629, 790, 943, 1393
SspMI	CTAG	2 cut(s)	192, 981
SstI	GAGCTC	1 cut(s)	923
StyD4I	CCNGG	2 cut(s)	904, 953
TaaI	ACNGT	4 cut(s)	269, 307, 398, 995
TaiI	ACGT	1 cut(s)	1196
TaqI	TCGA	5 cut(s)	82, 345, 673, 811, 1022
TatI	WGTACW	1 cut(s)	262
TfiI	GAWTC	3 cut(s)	182, 611, 1277
Tru1I	TTAA	2 cut(s)	291, 656
Tru9I	TTAA	2 cut(s)	291, 656
TscAI	CASTG	6 cut(s)	272, 401, 685, 763, 865, 1164
TseFI	GTSAC	2 cut(s)	21, 959
TseI	GCWGC	6 cut(s)	31, 106, 449, 631, 1123, 1126
Tsp45I	GTSAC	2 cut(s)	21, 959
TspDTI	ATGAA	7 cut(s)	60, 108, 174, 223, 402, 819, 1067
TspGWI	ACGGA	3 cut(s)	242, 679, 909
TspRI	CASTG	6 cut(s)	272, 401, 685, 763, 865, 1164
XapI	RAATTY	5 cut(s)	224, 745, 897, 1000, 1311
XhoI	CTCGAG	1 cut(s)	672
XspI	CTAG	2 cut(s)	192, 981
ZraI	GACGTC	1 cut(s)	1194

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.