Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0001286

Physical Location & Seq

Genomic Coordinates Reverse (-)

37999325 .. 38004714

Gene Structure 5390 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1291500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1320 bp

ATGCCGGCACAGAAGCGCACCCACGAGGCCATGGAAGATGATCCTCCGCAGAACTACCGAGAAAACAGCCACGAAACGCCGCAAGACGAAGAGGAGTCCGATCGAAGCCCGTTACAGAGCGCCGAAGAGAAAGACGAATTTGTCATAGTGAAATTGGCGGAAATTCGGAAAGAAGTCCAGTGCCCTATCTGTTTAGGGATCATTCGGAAAACAAGGACAGTGATGGAATGCCTGCATCGCTTCTGCAGGGAATGCATTGACAAGTCCATGCGCCTGGGGAACAATGAATGCCCTGCATGCCGCACCCATTGTGCTAGTCGTCGCTCTTTGAGAGATGACCCAAACTACGATGCCTTAATTGCAGCTATATATCCAGATATTGACAAGTATGAGGAAGAGGAATTGGCCTTTCATGAAGAGGAGAAGGCTCGCAATAAGCAGATCCAAGCTTCCATTGCCCAGACATTTCAACGACAAACCGAGGCACTAGGAAGGAAACGAACAACAGCTAAAGCCACTGCAGCTGCATTTATGAGGAGATCACGGGGTAGCTATCGAAATGCGAGGGGGAGAAGGAACTATAGAAATGTTGCTGAACATCATGGGTCCGATGACACTGAGGATATAAATGGTGATGATGGCAGTAGAGATTTATCTTCTGGTGATGAGCGCACAGAACCAAGACCAAAGAGATCCAAAAGATGGAGAGGAGGTCGATATTATCAGCCCTCGTCGGCAGCTGCTAATGCAGATAGTGATGATGAAAATGATTATGAAATGAACAGATCAGAGATTCTGGGTGCATCTGTGGGGCGTGTTGGCAGCTCAGAGAGGCTTTCCTGGGGAAAAGGTGGCATGCGGAGTCATACACGGTATGGCAGTTCAAGTGGTGGCAATGGTAAAAATGCCAGGAACATCCGCCTCTCTAGGTTGGTAGAGTATCTTCGGAACTCGGGAGATAATGATGACGAGTTGGACATCCACCTCCTGCTTGTTTCTCTCGATGAGCAAAGAATACCAAGTTTGCAGCGGCCATATCTTTGCTGCAGGTCTACCCTTTCAGTTGGACATTTATGTCAGTATGTAGCTCTCCGAACCGCTTCTCAAGCTGAAGAAATTGAAATATACTTGGTGAAAGAGCTGCATGCCGAATTCGGTCATTCAACTGCCACCAATATTAAAATTTCCAAGTCTCTTGTTCTAGATTCAAGCAAAGAAAACCTGCAACTTTTAAAGGAGGAAGAAACTTTGGCAGAACTTAGGACTCACAATCTCATTCCGGGCTATCTGATTTTGGCATATCAGAAGAAATGGAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0000152 GO:0001709 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010073 GO:0010074 GO:0010076 GO:0010077 GO:0010468 GO:0010492 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0019827 GO:0030154 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031519 GO:0032501 GO:0032502 GO:0032991 GO:0035102 GO:0040029 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045165 GO:0045814 GO:0045892 GO:0045934 GO:0048507 GO:0048519 GO:0048523 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0060255 GO:0065007 GO:0080090 GO:0098727 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1990234 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-C3HC4_2

Protein Analysis

440

Amino Acids

50.08

Weight (kDa)

6.39

Isoelectric Point (pI)

62.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-C3HC4_2	PF13923	60 - 100	1.3e-11	Zinc finger, C3HC4 type (RING finger)
zf-RING_2	PF13639	60 - 101	1.3e-07	Ring finger domain
zf-C3HC4	PF00097	61 - 100	9.6e-10	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001286)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G44280 AT5G44280 AT5G44280
fragaria_vesca	FvH4_3g08840 FvH4_3g08840 FvH4_3g08840 FvH4_3g08840 FvH4_6g26540 FvH4_6g26540 FvH4_6g26540 FvH4_6g26540 FvH4_6g26540 FvH4_6g26540 FvH4_6g26540 FvH4_6g26540
malus_domestica	MD05G1312000.v1.1 MD09G1244000.v1.1 MD10G1291500.v1.1 MD17G1235900.v1.1
prunus_persica	Prupe.3G139900_v2.0.a1 Prupe.3G139900_v2.0.a1 Prupe.3G139900_v2.0.a1 Prupe.3G139900_v2.0.a1 Prupe.3G139900_v2.0.a1 Prupe.4G050700_v2.0.a1 Prupe.4G050700_v2.0.a1 Prupe.4G050700_v2.0.a1
pyrus_communis	pycom05g28800 pycom09g16250 pycom10g24370
rosa_chinensis	RchiOBHm_Chr2g0127611 RchiOBHm_Chr5g0008911
rosa_laevigata	RLG00000018950 RLG00000031615
rosa_multiflora	Rmu_co8349609.1_g000001 Rmu_sc0004677.1_g000011 Rmu_ssc0000200.1_g000019
rosa_roxburghii	Rroxscaffold_1G00067100 Rroxscaffold_1G00067720 Rroxscaffold_2G00116570
rosa_rugosa	Rorug02G0272500 Rorug02G0272500 Rorug02G0272500 Rorug02G0272500 Rorug04G0440100
rosa_samantha	Rh2AG324500 Rh2BG333800 Rh2CG312000 Rh2DG352000 Rh5AG068800 Rh5BG065500 Rh5CG076700 Rh5DG064500
rosa_wichuraiana	Rw2G026350 Rw5G006440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	140, 1074
Acc36I	ACCTGC	2 cut(s)	1038, 1230
AccB7I	CCANNNNNTGG	1 cut(s)	702
AccI	GTMKAC	1 cut(s)	1052
AciI	CCGC	8 cut(s)	47, 80, 158, 301, 859, 919, 1030, 1098
AclWI	GGATC	4 cut(s)	35, 206, 436, 687
AcoI	YGGCCR	1 cut(s)	1031
AcsI	RAATTY	4 cut(s)	137, 162, 1151, 1182
AcuI	CTGAAG	1 cut(s)	1131
AfiI	CCNNNNNNNGG	1 cut(s)	702
AgsI	TTSAA	5 cut(s)	470, 885, 1121, 1164, 1209
AjnI	CCWGG	3 cut(s)	273, 839, 908
Alw26I	GTCTC	1 cut(s)	1197
AlwI	GGATC	4 cut(s)	35, 206, 436, 687
Ama87I	CYCGRG	1 cut(s)	952
AoxI	GGCC	3 cut(s)	27, 405, 1031
ApeKI	GCWGC	9 cut(s)	362, 521, 524, 737, 740, 822, 1027, 1044, 1141
ApoI	RAATTY	4 cut(s)	137, 162, 1151, 1182
Asp700I	GAANNNNTTC	1 cut(s)	1099
AspLEI	GCGC	4 cut(s)	18, 122, 273, 672
AspS9I	GGNCC	1 cut(s)	606
AsuC2I	CCSGG	1 cut(s)	1281
AsuHPI	GGTGA	3 cut(s)	644, 674, 1144
AvaI	CYCGRG	1 cut(s)	952
AvaII	GGWCC	1 cut(s)	606
BaeGI	GKGCMC	1 cut(s)	185
BarI	GAAGNNNNNNTAC	2 cut(s)	927, 959
BauI	CACGAG	1 cut(s)	23
BbvI	GCAGC	9 cut(s)	374, 511, 533, 727, 749, 834, 1031, 1039, 1128
BccI	CCATC	3 cut(s)	217, 632, 696
BciT130I	CCWGG	3 cut(s)	275, 841, 910
BcnI	CCSGG	1 cut(s)	1281
BcoDI	GTCTC	1 cut(s)	1197
BfaI	CTAG	4 cut(s)	315, 488, 927, 1202
BfmI	CTRYAG	4 cut(s)	244, 519, 580, 1045
BfoI	RGCGCY	1 cut(s)	123
BfuAI	ACCTGC	2 cut(s)	1038, 1230
Bme1390I	CCNGG	4 cut(s)	275, 841, 910, 1281
Bme18I	GGWCC	1 cut(s)	606
BmeT110I	CYCGRG	1 cut(s)	952
BmgT120I	GGNCC	1 cut(s)	606
BmiI	GGNNCC	1 cut(s)	607
BmrFI	CCNGG	4 cut(s)	275, 841, 910, 1281
BmsI	GCATC	3 cut(s)	244, 340, 812
BpuEI	CTTGAG	1 cut(s)	1089
BpuMI	CCSGG	1 cut(s)	1281
BsaJI	CCNNGG	4 cut(s)	30, 274, 480, 840
BsaXI	ACNNNNNCTCC	2 cut(s)	697, 727
Bsc4I	CCNNNNNNNGG	1 cut(s)	702
Bse118I	RCCGGY	1 cut(s)	4
Bse1I	ACTGG	1 cut(s)	178
Bse3DI	GCAATG	2 cut(s)	453, 901
BseBI	CCWGG	3 cut(s)	275, 841, 910
BseDI	CCNNGG	4 cut(s)	30, 274, 480, 840
BseGI	GGATG	2 cut(s)	915, 978
BseLI	CCNNNNNNNGG	1 cut(s)	702
BseMI	GCAATG	2 cut(s)	453, 901
BseMII	CTCAG	2 cut(s)	609, 840
BseNI	ACTGG	1 cut(s)	178
BseRI	GAGGAG	4 cut(s)	107, 434, 550, 723
BseSI	GKGCMC	1 cut(s)	185
BseXI	GCAGC	9 cut(s)	374, 511, 533, 727, 749, 834, 1031, 1039, 1128
Bsh1285I	CGRYCG	1 cut(s)	103
BshFI	GGCC	3 cut(s)	29, 407, 1033
BsiEI	CGRYCG	1 cut(s)	103
BsiHKCI	CYCGRG	1 cut(s)	952
BsiSI	CCGG	2 cut(s)	5, 1280
BslI	CCNNNNNNNGG	1 cut(s)	702
BsmAI	GTCTC	1 cut(s)	1197
BsmI	GAATGC	3 cut(s)	233, 257, 293
BsnI	GGCC	3 cut(s)	29, 407, 1033
BsoBI	CYCGRG	1 cut(s)	952
Bsp1286I	GDGCHC	1 cut(s)	185
Bsp143I	GATC	7 cut(s)	40, 100, 198, 441, 539, 692, 785
Bsp19I	CCATGG	1 cut(s)	30
BspACI	CCGC	8 cut(s)	47, 80, 158, 301, 859, 919, 1030, 1098
BspANI	GGCC	3 cut(s)	29, 407, 1033
BspCNI	CTCAG	2 cut(s)	610, 839
BspHI	TCATGA	1 cut(s)	412
BspLI	GGNNCC	1 cut(s)	607
BspMAI	CTGCAG	3 cut(s)	248, 523, 1049
BspMI	ACCTGC	2 cut(s)	1038, 1230
BspPI	GGATC	4 cut(s)	35, 206, 436, 687
BsrDI	GCAATG	2 cut(s)	453, 901
BsrFI	RCCGGY	1 cut(s)	4
BsrI	ACTGG	1 cut(s)	178
BssAI	RCCGGY	1 cut(s)	4
BssECI	CCNNGG	4 cut(s)	30, 274, 480, 840
BssMI	GATC	7 cut(s)	40, 100, 198, 441, 539, 692, 785
BssSI	CACGAG	1 cut(s)	23
BssT1I	CCWWGG	1 cut(s)	30
Bst2BI	CACGAG	1 cut(s)	23
Bst2UI	CCWGG	3 cut(s)	275, 841, 910
Bst4CI	ACNGT	2 cut(s)	220, 873
Bst6I	CTCTTC	4 cut(s)	84, 120, 390, 411
BstAPI	GCANNNNNTGC	1 cut(s)	252
BstC8I	GCNNGC	6 cut(s)	6, 233, 298, 430, 857, 1146
BstDEI	CTNAG	3 cut(s)	618, 826, 1259
BstDSI	CCRYGG	1 cut(s)	30
BstF5I	GGATG	2 cut(s)	915, 978
BstH2I	RGCGCY	1 cut(s)	123
BstHHI	GCGC	4 cut(s)	18, 122, 273, 672
BstKTI	GATC	7 cut(s)	43, 103, 201, 444, 542, 695, 788
BstMAI	GTCTC	1 cut(s)	1197
BstMBI	GATC	7 cut(s)	40, 100, 198, 441, 539, 692, 785
BstMCI	CGRYCG	1 cut(s)	103
BstMWI	GCNNNNNNNGC	8 cut(s)	237, 252, 297, 359, 455, 521, 746, 1106
BstNI	CCWGG	3 cut(s)	275, 841, 910
BstNSI	RCATGY	3 cut(s)	300, 859, 1148
BstSCI	CCNGG	4 cut(s)	273, 839, 908, 1279
BstSFI	CTRYAG	4 cut(s)	244, 519, 580, 1045
BstSLI	GKGCMC	1 cut(s)	185
BstV1I	GCAGC	9 cut(s)	374, 511, 533, 727, 749, 834, 1031, 1039, 1128
BstX2I	RGATCY	2 cut(s)	441, 692
BstXI	CCANNNNNNTGG	1 cut(s)	274
BstYI	RGATCY	2 cut(s)	441, 692
BsuRI	GGCC	3 cut(s)	29, 407, 1033
BtgI	CCRYGG	1 cut(s)	30
BtgZI	GCGATG	1 cut(s)	221
BtsCI	GGATG	2 cut(s)	915, 978
BtsI	GCAGTG	1 cut(s)	516
BtsIMutI	CAGTG	4 cut(s)	185, 225, 516, 615
BveI	ACCTGC	2 cut(s)	1038, 1230
Cac8I	GCNNGC	6 cut(s)	6, 233, 298, 430, 857, 1146
CciI	TCATGA	1 cut(s)	412
CfoI	GCGC	4 cut(s)	18, 122, 273, 672
Cfr10I	RCCGGY	1 cut(s)	4
Cfr13I	GGNCC	1 cut(s)	606
CviAII	CATG	7 cut(s)	31, 268, 297, 413, 602, 856, 1145
DdeI	CTNAG	3 cut(s)	618, 826, 1259
DpnI	GATC	7 cut(s)	42, 102, 200, 443, 541, 694, 787
DpnII	GATC	7 cut(s)	40, 100, 198, 441, 539, 692, 785
DraI	TTTAAA	1 cut(s)	1233
DrdI	GACNNNNNNGTC	2 cut(s)	140, 1074
DseDI	GACNNNNNNGTC	2 cut(s)	140, 1074
EaeI	YGGCCR	1 cut(s)	1031
Eam1104I	CTCTTC	4 cut(s)	84, 120, 390, 411
EarI	CTCTTC	4 cut(s)	84, 120, 390, 411
EciI	GGCGGA	2 cut(s)	173, 908
Eco130I	CCWWGG	1 cut(s)	30
Eco47I	GGWCC	1 cut(s)	606
Eco57I	CTGAAG	1 cut(s)	1131
Eco88I	CYCGRG	1 cut(s)	952
EcoRI	GAATTC	1 cut(s)	1151
EcoRII	CCWGG	3 cut(s)	273, 839, 908
EcoT14I	CCWWGG	1 cut(s)	30
EcoT22I	ATGCAT	1 cut(s)	257
ErhI	CCWWGG	1 cut(s)	30
FaeI	CATG	7 cut(s)	34, 271, 300, 416, 605, 859, 1148
FatI	CATG	7 cut(s)	30, 267, 296, 412, 601, 855, 1144
FblI	GTMKAC	1 cut(s)	1052
FokI	GGATG	2 cut(s)	902, 965
FspBI	CTAG	4 cut(s)	315, 488, 927, 1202
GlaI	GCGC	4 cut(s)	17, 121, 272, 671
HaeII	RGCGCY	1 cut(s)	123
HaeIII	GGCC	3 cut(s)	29, 407, 1033
HapII	CCGG	2 cut(s)	5, 1280
HhaI	GCGC	4 cut(s)	18, 122, 273, 672
Hin1II	CATG	7 cut(s)	34, 271, 300, 416, 605, 859, 1148
Hin6I	GCGC	4 cut(s)	16, 120, 271, 670
HinP1I	GCGC	4 cut(s)	16, 120, 271, 670
HindIII	AAGCTT	1 cut(s)	447
HinfI	GANTC	5 cut(s)	95, 793, 862, 1205, 1264
HpaII	CCGG	2 cut(s)	5, 1280
HphI	GGTGA	3 cut(s)	644, 674, 1144
Hpy166II	GTNNAC	1 cut(s)	1053
Hpy188III	TCNNGA	5 cut(s)	374, 413, 954, 1001, 1202
Hpy8I	GTNNAC	1 cut(s)	1053
Hpy99I	CGWCG	2 cut(s)	324, 736
HpyAV	CCTTC	3 cut(s)	418, 486, 567
HpyCH4III	ACNGT	2 cut(s)	220, 873
HpyF10VI	GCNNNNNNNGC	8 cut(s)	237, 252, 297, 359, 455, 521, 746, 1106
HpyF3I	CTNAG	3 cut(s)	618, 826, 1259
Hsp92II	CATG	7 cut(s)	34, 271, 300, 416, 605, 859, 1148
HspAI	GCGC	4 cut(s)	16, 120, 271, 670
KroI	GCCGGC	1 cut(s)	4
KroNI	GCCGGC	1 cut(s)	6
Kzo9I	GATC	7 cut(s)	40, 100, 198, 441, 539, 692, 785
Lsp1109I	GCAGC	9 cut(s)	374, 511, 533, 727, 749, 834, 1031, 1039, 1128
LweI	GCATC	3 cut(s)	244, 340, 812
MaeI	CTAG	4 cut(s)	315, 488, 927, 1202
MaeIII	GTNAC	1 cut(s)	111
MalI	GATC	7 cut(s)	42, 102, 200, 443, 541, 694, 787
MboI	GATC	7 cut(s)	40, 100, 198, 441, 539, 692, 785
MflI	RGATCY	2 cut(s)	441, 692
MhlI	GDGCHC	1 cut(s)	185
MluCI	AATT	8 cut(s)	137, 152, 162, 357, 401, 1116, 1151, 1182
MlyI	GAGTC	3 cut(s)	104, 871, 1258
MmeI	TCCRAC	2 cut(s)	954, 1045
Mph1103I	ATGCAT	1 cut(s)	257
MroNI	GCCGGC	1 cut(s)	4
MroXI	GAANNNNTTC	1 cut(s)	1099
MseI	TTAA	3 cut(s)	356, 1179, 1232
MspA1I	CMGCKG	3 cut(s)	524, 740, 1030
MspI	CCGG	2 cut(s)	5, 1280
MspR9I	CCNGG	4 cut(s)	275, 841, 910, 1281
Mva1269I	GAATGC	3 cut(s)	233, 257, 293
MvaI	CCWGG	3 cut(s)	275, 841, 910
MwoI	GCNNNNNNNGC	8 cut(s)	237, 252, 297, 359, 455, 521, 746, 1106
NaeI	GCCGGC	1 cut(s)	6
NciI	CCSGG	1 cut(s)	1281
NcoI	CCATGG	1 cut(s)	30
NdeII	GATC	7 cut(s)	40, 100, 198, 441, 539, 692, 785
NgoMIV	GCCGGC	1 cut(s)	4
NlaIII	CATG	7 cut(s)	34, 271, 300, 416, 605, 859, 1148
NlaIV	GGNNCC	1 cut(s)	607
NsiI	ATGCAT	1 cut(s)	257
NspI	RCATGY	3 cut(s)	300, 859, 1148
PaeI	GCATGC	3 cut(s)	300, 859, 1148
PagI	TCATGA	1 cut(s)	412
PctI	GAATGC	3 cut(s)	233, 257, 293
PdiI	GCCGGC	1 cut(s)	6
PdmI	GAANNNNTTC	1 cut(s)	1099
PfeI	GAWTC	2 cut(s)	793, 1205
PflMI	CCANNNNNTGG	1 cut(s)	702
Ple19I	CGATCG	1 cut(s)	103
PleI	GAGTC	3 cut(s)	103, 870, 1258
PpsI	GAGTC	3 cut(s)	103, 870, 1258
Psp6I	CCWGG	3 cut(s)	273, 839, 908
PspGI	CCWGG	3 cut(s)	273, 839, 908
PspN4I	GGNNCC	1 cut(s)	607
PspPI	GGNCC	1 cut(s)	606
PstI	CTGCAG	3 cut(s)	248, 523, 1049
PsuI	RGATCY	2 cut(s)	441, 692
PvuI	CGATCG	1 cut(s)	103
PvuII	CAGCTG	2 cut(s)	524, 740
SaqAI	TTAA	3 cut(s)	356, 1179, 1232
Sau3AI	GATC	7 cut(s)	40, 100, 198, 441, 539, 692, 785
Sau96I	GGNCC	1 cut(s)	606
SchI	GAGTC	3 cut(s)	104, 871, 1258
ScrFI	CCNGG	4 cut(s)	275, 841, 910, 1281
SduI	GDGCHC	1 cut(s)	185
SfaNI	GCATC	3 cut(s)	244, 340, 812
SfcI	CTRYAG	4 cut(s)	244, 519, 580, 1045
SinI	GGWCC	1 cut(s)	606
SmlI	CTYRAG	1 cut(s)	1104
SmoI	CTYRAG	1 cut(s)	1104
SphI	GCATGC	3 cut(s)	300, 859, 1148
Sse9I	AATT	8 cut(s)	137, 152, 162, 357, 401, 1116, 1151, 1182
SsiI	CCGC	8 cut(s)	47, 80, 158, 301, 859, 919, 1030, 1098
SspI	AATATT	1 cut(s)	1177
SspMI	CTAG	4 cut(s)	315, 488, 927, 1202
StyD4I	CCNGG	4 cut(s)	273, 839, 908, 1279
StyI	CCWWGG	1 cut(s)	30
TaaI	ACNGT	2 cut(s)	220, 873
TaqI	TCGA	4 cut(s)	103, 556, 715, 1002
TaqII	GACCGA	1 cut(s)	1145
TasI	AATT	8 cut(s)	137, 152, 162, 357, 401, 1116, 1151, 1182
TauI	GCSGC	3 cut(s)	82, 303, 1033
TfiI	GAWTC	2 cut(s)	793, 1205
Tru1I	TTAA	3 cut(s)	356, 1179, 1232
Tru9I	TTAA	3 cut(s)	356, 1179, 1232
TscAI	CASTG	4 cut(s)	185, 225, 523, 622
TseI	GCWGC	9 cut(s)	362, 521, 524, 737, 740, 822, 1027, 1044, 1141
TspDTI	ATGAA	6 cut(s)	300, 401, 429, 777, 789, 794
TspRI	CASTG	4 cut(s)	185, 225, 523, 622
Van91I	CCANNNNNTGG	1 cut(s)	702
VpaK11BI	GGWCC	1 cut(s)	606
XapI	RAATTY	4 cut(s)	137, 162, 1151, 1182
XbaI	TCTAGA	1 cut(s)	1201
XceI	RCATGY	3 cut(s)	300, 859, 1148
XmiI	GTMKAC	1 cut(s)	1052
XmnI	GAANNNNTTC	1 cut(s)	1099
XspI	CTAG	4 cut(s)	315, 488, 927, 1202
Zsp2I	ATGCAT	1 cut(s)	257

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.