MD10G1020800.v1.1

Protein DA1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008106
Physical Location & Seq
Reverse (-)
2546853 .. 2547398
546 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1020800.v1.1.491

Sequence Viewer

Length: 546 bp
ATGTATCTTGGTGATTATTTGCTATGTCAACAGTGTCAAAAGTTGGCAATCAGGAGTACATACAAATGCCAATCCCTCTACAATGAAGTAGAAAAATTCTGTCACGATTTAAACATGAGGTTGCAAATTCCTGTGGACTTGGTGGACAGGCGAACAGTTCTCATCTGGCGTGGCCTTCCTCGGTTTGTAATGGGATCATCGCTAGCGAAAGTGATGATGCAAGCATGGATAAGGATTAACGGATATCCAAATCTAAGACAGGAAGTGCAAGAAGGTATCTGTCTGGCCTTCTCGCGCATGTGGCTGGAGTCTCAGCAGATGCATTCTCGGTCTAGTACTGATCATGATGCCTCCTCCTCTAGTTTTGTTGGATCTAAACAACTGTCCGAATTTGAGAAGAAGCTTCTTGAATACAGGAAACGAACAATTCGGTTATTGGAGTATGGAGAAGCATCCACAGTAGCTATCAAAGCAGTGCGCAAGCACGGTCTAGGGAATACCCTTGAACATATTCGAACCACAGGAAATTTTCCTCCTGTAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DA1-like LIM NB-ARC TIR

Protein Analysis

182

Amino Acids

20.99

Weight (kDa)

9.28

Isoelectric Point (pI)

43.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DA1-like PF12315 51 - 173 2.2e-27 Protein DA1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 479
AccII CGCG 1 cut(s) 295
AclWI GGATC 2 cut(s) 202, 379
AcsI RAATTY 4 cut(s) 95, 126, 389, 526
AfaI GTAC 2 cut(s) 58, 337
AgsI TTSAA 2 cut(s) 410, 506
AluBI AGCT 2 cut(s) 403, 464
AluI AGCT 2 cut(s) 403, 464
Alw26I GTCTC 1 cut(s) 315
AlwI GGATC 2 cut(s) 202, 379
AoxI GGCC 2 cut(s) 172, 285
ApoI RAATTY 4 cut(s) 95, 126, 389, 526
Asp700I GAANNNNTTC 1 cut(s) 510
AspLEI GCGC 2 cut(s) 297, 480
AsuHPI GGTGA 1 cut(s) 23
AsuII TTCGAA 1 cut(s) 514
AsuNHI GCTAGC 1 cut(s) 202
BclI TGATCA 1 cut(s) 340
BcoDI GTCTC 1 cut(s) 315
BfaI CTAG 4 cut(s) 203, 333, 360, 491
BfmI CTRYAG 1 cut(s) 537
BmcAI AGTACT 1 cut(s) 337
BmsI GCATC 4 cut(s) 207, 309, 337, 461
BmtI GCTAGC 1 cut(s) 206
BpmI CTGGAG 1 cut(s) 326
Bpu14I TTCGAA 1 cut(s) 514
BsaJI CCNNGG 1 cut(s) 179
BseDI CCNNGG 1 cut(s) 179
BseGI GGATG 1 cut(s) 452
BseMII CTCAG 1 cut(s) 326
BseRI GAGGAG 2 cut(s) 343, 346
Bsh1236I CGCG 1 cut(s) 295
BshFI GGCC 2 cut(s) 174, 287
BsmAI GTCTC 1 cut(s) 315
BsmI GAATGC 1 cut(s) 322
BsnI GGCC 2 cut(s) 174, 287
Bsp119I TTCGAA 1 cut(s) 514
Bsp143I GATC 3 cut(s) 194, 340, 371
BspANI GGCC 2 cut(s) 174, 287
BspCNI CTCAG 1 cut(s) 325
BspFNI CGCG 1 cut(s) 295
BspHI TCATGA 1 cut(s) 343
BspOI GCTAGC 1 cut(s) 206
BspPI GGATC 2 cut(s) 202, 379
BspT104I TTCGAA 1 cut(s) 514
BssECI CCNNGG 1 cut(s) 179
BssMI GATC 3 cut(s) 194, 340, 371
Bst4CI ACNGT 5 cut(s) 33, 157, 384, 460, 488
BstBI TTCGAA 1 cut(s) 514
BstC8I GCNNGC 3 cut(s) 204, 222, 482
BstDEI CTNAG 2 cut(s) 254, 312
BstF5I GGATG 1 cut(s) 452
BstFNI CGCG 1 cut(s) 295
BstHHI GCGC 2 cut(s) 297, 480
BstKTI GATC 3 cut(s) 197, 343, 374
BstMAI GTCTC 1 cut(s) 315
BstMBI GATC 3 cut(s) 194, 340, 371
BstMWI GCNNNNNNNGC 2 cut(s) 301, 470
BstNSI RCATGY 1 cut(s) 301
BstSFI CTRYAG 1 cut(s) 537
BstUI CGCG 1 cut(s) 295
BstX2I RGATCY 1 cut(s) 371
BstYI RGATCY 1 cut(s) 371
BsuRI GGCC 2 cut(s) 174, 287
BtgZI GCGATG 1 cut(s) 183
BtsCI GGATG 1 cut(s) 452
BtsI GCAGTG 1 cut(s) 480
BtsIMutI CAGTG 2 cut(s) 38, 480
Cac8I GCNNGC 3 cut(s) 204, 222, 482
CciI TCATGA 1 cut(s) 343
CfoI GCGC 2 cut(s) 297, 480
Csp6I GTAC 2 cut(s) 57, 336
CviAII CATG 4 cut(s) 115, 225, 298, 344
CviJI RGCY 5 cut(s) 174, 287, 304, 403, 464
CviKI_1 RGCY 5 cut(s) 174, 287, 304, 403, 464
CviQI GTAC 2 cut(s) 57, 336
DdeI CTNAG 2 cut(s) 254, 312
DpnI GATC 3 cut(s) 196, 342, 373
DpnII GATC 3 cut(s) 194, 340, 371
DraI TTTAAA 1 cut(s) 111
Eco32I GATATC 1 cut(s) 245
EcoRV GATATC 1 cut(s) 245
EcoT22I ATGCAT 1 cut(s) 324
FaeI CATG 4 cut(s) 118, 228, 301, 347
FaiI YATR 8 cut(s) 25, 61, 116, 226, 299, 345, 444, 510
FatI CATG 4 cut(s) 114, 224, 297, 343
FbaI TGATCA 1 cut(s) 340
FokI GGATG 1 cut(s) 439
FspBI CTAG 4 cut(s) 203, 333, 360, 491
FspI TGCGCA 1 cut(s) 479
GlaI GCGC 2 cut(s) 296, 479
GsuI CTGGAG 1 cut(s) 326
HaeIII GGCC 2 cut(s) 174, 287
HhaI GCGC 2 cut(s) 297, 480
Hin1II CATG 4 cut(s) 118, 228, 301, 347
Hin6I GCGC 2 cut(s) 295, 478
HinP1I GCGC 2 cut(s) 295, 478
HincII GTYRAC 1 cut(s) 29
HindII GTYRAC 1 cut(s) 29
HindIII AAGCTT 1 cut(s) 401
HinfI GANTC 1 cut(s) 308
HphI GGTGA 1 cut(s) 23
Hpy166II GTNNAC 3 cut(s) 29, 136, 145
Hpy188I TCNGA 1 cut(s) 388
Hpy188III TCNNGA 4 cut(s) 52, 104, 344, 407
Hpy8I GTNNAC 3 cut(s) 29, 136, 145
HpyAV CCTTC 3 cut(s) 185, 266, 298
HpyCH4III ACNGT 5 cut(s) 33, 157, 384, 460, 488
HpyCH4V TGCA 4 cut(s) 124, 220, 268, 322
HpyF10VI GCNNNNNNNGC 2 cut(s) 301, 470
HpyF3I CTNAG 2 cut(s) 254, 312
Hsp92II CATG 4 cut(s) 118, 228, 301, 347
HspAI GCGC 2 cut(s) 295, 478
Ksp22I TGATCA 1 cut(s) 340
Kzo9I GATC 3 cut(s) 194, 340, 371
LpnPI CCDG 9 cut(s) 37, 133, 144, 151, 245, 269, 290, 400, 507
LweI GCATC 4 cut(s) 207, 309, 337, 461
MaeI CTAG 4 cut(s) 203, 333, 360, 491
MaeIII GTNAC 1 cut(s) 101
MalI GATC 3 cut(s) 196, 342, 373
MboI GATC 3 cut(s) 194, 340, 371
MboII GAAGA 1 cut(s) 409
MflI RGATCY 1 cut(s) 371
MluCI AATT 5 cut(s) 95, 126, 389, 426, 526
MlyI GAGTC 1 cut(s) 317
MmeI TCCRAC 1 cut(s) 349
MnlI CCTC 7 cut(s) 86, 111, 189, 361, 364, 367, 543
Mph1103I ATGCAT 1 cut(s) 324
MroXI GAANNNNTTC 1 cut(s) 510
MseI TTAA 2 cut(s) 110, 237
MslI CAYNNNNRTG 1 cut(s) 64
Mva1269I GAATGC 1 cut(s) 322
MvnI CGCG 1 cut(s) 295
MwoI GCNNNNNNNGC 2 cut(s) 301, 470
NdeII GATC 3 cut(s) 194, 340, 371
NheI GCTAGC 1 cut(s) 202
NlaIII CATG 4 cut(s) 118, 228, 301, 347
NmuCI GTSAC 1 cut(s) 101
NsbI TGCGCA 1 cut(s) 479
NsiI ATGCAT 1 cut(s) 324
NspI RCATGY 1 cut(s) 301
NspV TTCGAA 1 cut(s) 514
PagI TCATGA 1 cut(s) 343
PctI GAATGC 1 cut(s) 322
PdmI GAANNNNTTC 1 cut(s) 510
PleI GAGTC 1 cut(s) 316
PpsI GAGTC 1 cut(s) 316
PsuI RGATCY 1 cut(s) 371
RsaI GTAC 2 cut(s) 58, 337
RsaNI GTAC 2 cut(s) 57, 336
RseI CAYNNNNRTG 1 cut(s) 64
SaqAI TTAA 2 cut(s) 110, 237
Sau3AI GATC 3 cut(s) 194, 340, 371
ScaI AGTACT 1 cut(s) 337
SchI GAGTC 1 cut(s) 317
SetI ASST 4 cut(s) 122, 277, 405, 466
SfaNI GCATC 4 cut(s) 207, 309, 337, 461
SfcI CTRYAG 1 cut(s) 537
SfuI TTCGAA 1 cut(s) 514
SmiMI CAYNNNNRTG 1 cut(s) 64
Sse9I AATT 5 cut(s) 95, 126, 389, 426, 526
SspMI CTAG 4 cut(s) 203, 333, 360, 491
TaaI ACNGT 5 cut(s) 33, 157, 384, 460, 488
TaqI TCGA 1 cut(s) 514
TaqII GACCGA 1 cut(s) 318
TasI AATT 5 cut(s) 95, 126, 389, 426, 526
TatI WGTACW 2 cut(s) 56, 335
Tru1I TTAA 2 cut(s) 110, 237
Tru9I TTAA 2 cut(s) 110, 237
TscAI CASTG 2 cut(s) 38, 480
TseFI GTSAC 1 cut(s) 101
Tsp45I GTSAC 1 cut(s) 101
TspDTI ATGAA 1 cut(s) 99
TspGWI ACGGA 1 cut(s) 255
TspRI CASTG 2 cut(s) 38, 480
XapI RAATTY 4 cut(s) 95, 126, 389, 526
XceI RCATGY 1 cut(s) 301
XmnI GAANNNNTTC 1 cut(s) 510
XspI CTAG 4 cut(s) 203, 333, 360, 491
ZrmI AGTACT 1 cut(s) 337
Zsp2I ATGCAT 1 cut(s) 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.