Rh2BG242000

DA1-related 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0008106
Physical Location & Seq
Forward (+)
24260233 .. 24262849
2617 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG242000.1

Sequence Viewer

Length: 1068 bp
ATGTGTCTGGCCAACAAGAACTTCTTGGCCGGCCAGCTTGATTGGAGTTCATGGTCAGAGACCGAGTTCATCGAAAAAGTTGTAGACAAACTGTATAACAAACTGGTATGCGCTGGCTGCAACGGAAGGGCTGGTGATGGCCTCAACTGCTTGGGTGCTGTTTGGCATCGAGCATGTCTTCTGACTCTTCTCTGCAAAGGTTGCAACCATCCGTTTACTGATCTAGAGATTCGGGAATGTGGGGATCAGCAGCCTTACCATGAACGCTGCTATGAGCTAGAATATCTTGTATGCAATGTTTGCAAGAAATCTATCCAAGATGGTCAAGTTGAGTACAAGGAATATCCATTCTGGCGACGAAGATATTGTTCCTCCCATGAAAATGATGGGACTCCTAGGTGTTGTAGCTGTGAGAGATTGAAGCCAAGGGGCACAACATATTATAAGCTTGATGATGGTCGAAAACTATGTCAGGAGTGTAAAGAGTCCATTATCAATACTGGTCAAGGACACCGATCCATTTGCCTTGAAGTACAAAAATTTTATAAGAAGTTAAATGTGAAAGTAGAGAAAATTCCAGTGCGGTTGGTCGGCAGACAAGTCATAATCGATGCCATGAAGGAGGGAAATGATGATTCTGATCGCCAATTGCCGGTAATAAGAGCAGTCGGCTTGTTTGAAAAGAGTCTTACCAGCGCTATTATGGGGAGGCCGAGCCAGCTTTGCTGTGAAGTTAAAGAAATTTTAATCTGGTACGGCCTTCCTAGAATATTGACTGGGTCACTTCTAGCTCAACAGATGATGAGGGCATGGCTAAGGCTTAATTGCTGTCCGAATATGAGCCCTGAGGTTGAAAAAGGTATCTGCCGATATTTTGCACATAGGTGGTTGGAGTACTCTTCTAGTTCCTATTCATCTAAATTGTCTGAGTTTGAGGAGAAGCTTTGTAGGAAACGCCAAACTGAGTTAGGTTGTGAAGCATTACTGACAGCTAAGAAAGCTGTGTGCAAGCATGGTCTAGGAATGACACTTGAAACAATCCGAAATACAGGAAAGTTTCCATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0032182 GO:0043130 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DA1-like LIM

Protein Analysis

355

Amino Acids

40.73

Weight (kDa)

8.66

Isoelectric Point (pI)

41.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DA1-like PF12315 185 - 307 6.2e-25 Protein DA1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 444, 546
AccI GTMKAC 1 cut(s) 84
AciI CCGC 1 cut(s) 583
AclWI GGATC 2 cut(s) 252, 510
AcoI YGGCCR 3 cut(s) 9, 27, 31
AcsI RAATTY 3 cut(s) 539, 573, 741
AfaI GTAC 4 cut(s) 335, 534, 755, 896
AfeI AGCGCT 1 cut(s) 697
AfiI CCNNNNNNNGG 1 cut(s) 652
AgsI TTSAA 5 cut(s) 421, 530, 680, 854, 1034
AjuI GAANNNNNNNTTGG 1 cut(s) 37
AleI CACNNNNGTG 1 cut(s) 883
AluBI AGCT 9 cut(s) 37, 277, 408, 448, 721, 791, 943, 992, 1001
AluI AGCT 9 cut(s) 37, 277, 408, 448, 721, 791, 943, 992, 1001
Alw26I GTCTC 1 cut(s) 53
AlwI GGATC 2 cut(s) 252, 510
Aor51HI AGCGCT 1 cut(s) 697
AoxI GGCC 6 cut(s) 9, 27, 31, 139, 710, 757
ApeKI GCWGC 3 cut(s) 117, 250, 267
ApoI RAATTY 3 cut(s) 539, 573, 741
ArsI GACNNNNNNTTYG 2 cut(s) 454, 486
AspA2I CCTAGG 1 cut(s) 395
AspLEI GCGC 2 cut(s) 113, 698
AsuHPI GGTGA 1 cut(s) 146
AvrII CCTAGG 1 cut(s) 395
AxyI CCTNAGG 1 cut(s) 846
BaeGI GKGCMC 1 cut(s) 434
BalI TGGCCA 1 cut(s) 11
BanII GRGCYC 1 cut(s) 845
BbsI GAAGAC 1 cut(s) 170
BbvI GCAGC 3 cut(s) 104, 254, 262
BccI CCATC 5 cut(s) 131, 216, 314, 380, 449
BceAI ACGGC 1 cut(s) 772
BcgI CGANNNNNNTGC 2 cut(s) 504, 538
BcoDI GTCTC 1 cut(s) 53
BfaI CTAG 7 cut(s) 224, 278, 396, 765, 788, 903, 1019
BfoI RGCGCY 1 cut(s) 699
BisI GCNGC 3 cut(s) 118, 251, 268
BlnI CCTAGG 1 cut(s) 395
BlsI GCNGC 3 cut(s) 119, 252, 269
BmcAI AGTACT 1 cut(s) 896
BmrI ACTGGG 1 cut(s) 786
BmsI GCATC 2 cut(s) 175, 601
BmuI ACTGGG 1 cut(s) 786
BpiI GAAGAC 1 cut(s) 170
Bpu10I CCTNAGC 1 cut(s) 815
Bsa29I ATCGAT 1 cut(s) 609
BsaBI GATNNNNATC 1 cut(s) 639
BsaI GGTCTC 1 cut(s) 53
BsaJI CCNNGG 2 cut(s) 395, 425
Bsc4I CCNNNNNNNGG 1 cut(s) 652
Bse118I RCCGGY 2 cut(s) 29, 652
Bse1I ACTGG 4 cut(s) 108, 505, 578, 781
Bse21I CCTNAGG 1 cut(s) 846
Bse3DI GCAATG 1 cut(s) 301
Bse8I GATNNNNATC 1 cut(s) 639
BseCI ATCGAT 1 cut(s) 609
BseDI CCNNGG 2 cut(s) 395, 425
BseGI GGATG 1 cut(s) 208
BseJI GATNNNNATC 1 cut(s) 639
BseLI CCNNNNNNNGG 1 cut(s) 652
BseMI GCAATG 1 cut(s) 301
BseMII CTCAG 3 cut(s) 837, 918, 954
BseNI ACTGG 4 cut(s) 108, 505, 578, 781
BseRI GAGGAG 1 cut(s) 950
BseSI GKGCMC 1 cut(s) 434
BseXI GCAGC 3 cut(s) 104, 254, 262
BshFI GGCC 6 cut(s) 11, 29, 33, 141, 712, 759
BshVI ATCGAT 1 cut(s) 609
BsiSI CCGG 2 cut(s) 30, 653
BslFI GGGAC 1 cut(s) 403
BslI CCNNNNNNNGG 1 cut(s) 652
BsmAI GTCTC 1 cut(s) 53
BsmFI GGGAC 1 cut(s) 403
BsnI GGCC 6 cut(s) 11, 29, 33, 141, 712, 759
Bso31I GGTCTC 1 cut(s) 53
Bsp1286I GDGCHC 2 cut(s) 434, 845
Bsp143I GATC 4 cut(s) 220, 244, 515, 640
BspACI CCGC 1 cut(s) 583
BspANI GGCC 6 cut(s) 11, 29, 33, 141, 712, 759
BspCNI CTCAG 3 cut(s) 838, 919, 955
BspDI ATCGAT 1 cut(s) 609
BspPI GGATC 2 cut(s) 252, 510
BspTNI GGTCTC 1 cut(s) 53
BsrDI GCAATG 1 cut(s) 301
BsrFI RCCGGY 2 cut(s) 29, 652
BsrI ACTGG 4 cut(s) 108, 505, 578, 781
BssAI RCCGGY 2 cut(s) 29, 652
BssECI CCNNGG 2 cut(s) 395, 425
BssMI GATC 4 cut(s) 220, 244, 515, 640
BssT1I CCWWGG 2 cut(s) 395, 425
Bst4CI ACNGT 1 cut(s) 93
Bst6I CTCTTC 2 cut(s) 192, 904
BstAPI GCANNNNNTGC 2 cut(s) 201, 300
BstC8I GCNNGC 5 cut(s) 31, 35, 115, 719, 1010
BstDEI CTNAG 5 cut(s) 815, 846, 927, 963, 993
BstF5I GGATG 1 cut(s) 208
BstH2I RGCGCY 1 cut(s) 699
BstHHI GCGC 2 cut(s) 113, 698
BstKTI GATC 4 cut(s) 223, 247, 518, 643
BstMAI GTCTC 1 cut(s) 53
BstMBI GATC 4 cut(s) 220, 244, 515, 640
BstMWI GCNNNNNNNGC 7 cut(s) 117, 147, 201, 300, 718, 723, 998
BstNSI RCATGY 1 cut(s) 177
BstSLI GKGCMC 1 cut(s) 434
BstV1I GCAGC 3 cut(s) 104, 254, 262
BstV2I GAAGAC 1 cut(s) 170
Bsu15I ATCGAT 1 cut(s) 609
Bsu36I CCTNAGG 1 cut(s) 846
BsuRI GGCC 6 cut(s) 11, 29, 33, 141, 712, 759
BsuTUI ATCGAT 1 cut(s) 609
BtsCI GGATG 1 cut(s) 208
BtsIMutI CAGTG 1 cut(s) 585
Cac8I GCNNGC 5 cut(s) 31, 35, 115, 719, 1010
CfoI GCGC 2 cut(s) 113, 698
Cfr10I RCCGGY 2 cut(s) 29, 652
ClaI ATCGAT 1 cut(s) 609
Csp6I GTAC 4 cut(s) 334, 533, 754, 895
CviAII CATG 7 cut(s) 51, 174, 260, 377, 616, 810, 1013
CviQI GTAC 4 cut(s) 334, 533, 754, 895
DdeI CTNAG 5 cut(s) 815, 846, 927, 963, 993
DpnI GATC 4 cut(s) 222, 246, 517, 642
DpnII GATC 4 cut(s) 220, 244, 515, 640
EaeI YGGCCR 3 cut(s) 9, 27, 31
Eam1104I CTCTTC 2 cut(s) 192, 904
EarI CTCTTC 2 cut(s) 192, 904
Eco130I CCWWGG 2 cut(s) 395, 425
Eco24I GRGCYC 1 cut(s) 845
Eco31I GGTCTC 1 cut(s) 53
Eco47III AGCGCT 1 cut(s) 697
Eco81I CCTNAGG 1 cut(s) 846
EcoT14I CCWWGG 2 cut(s) 395, 425
EcoT38I GRGCYC 1 cut(s) 845
ErhI CCWWGG 2 cut(s) 395, 425
FaeI CATG 7 cut(s) 54, 177, 263, 380, 619, 813, 1016
FalI AAGNNNNNCTT 2 cut(s) 8, 40
FaqI GGGAC 1 cut(s) 403
FatI CATG 7 cut(s) 50, 173, 259, 376, 615, 809, 1012
FblI GTMKAC 1 cut(s) 84
Fnu4HI GCNGC 3 cut(s) 118, 251, 268
FokI GGATG 1 cut(s) 195
FriOI GRGCYC 1 cut(s) 845
FseI GGCCGGCC 1 cut(s) 33
Fsp4HI GCNGC 3 cut(s) 118, 251, 268
FspBI CTAG 7 cut(s) 224, 278, 396, 765, 788, 903, 1019
GlaI GCGC 2 cut(s) 112, 697
GluI GCNGC 3 cut(s) 118, 251, 268
HaeII RGCGCY 1 cut(s) 699
HaeIII GGCC 6 cut(s) 11, 29, 33, 141, 712, 759
HapII CCGG 2 cut(s) 30, 653
HhaI GCGC 2 cut(s) 113, 698
Hin1II CATG 7 cut(s) 54, 177, 263, 380, 619, 813, 1016
Hin6I GCGC 2 cut(s) 111, 696
HinP1I GCGC 2 cut(s) 111, 696
HindIII AAGCTT 2 cut(s) 446, 941
HinfI GANTC 6 cut(s) 184, 229, 391, 485, 635, 685
HpaII CCGG 2 cut(s) 30, 653
HphI GGTGA 1 cut(s) 146
Hpy166II GTNNAC 2 cut(s) 85, 216
Hpy188I TCNGA 6 cut(s) 58, 183, 640, 834, 928, 1043
Hpy188III TCNNGA 3 cut(s) 224, 233, 473
Hpy8I GTNNAC 2 cut(s) 85, 216
Hpy99I CGWCG 1 cut(s) 360
HpyAV CCTTC 3 cut(s) 120, 613, 770
HpyCH4III ACNGT 1 cut(s) 93
HpyCH4V TGCA 7 cut(s) 120, 195, 204, 294, 303, 878, 1008
HpyF10VI GCNNNNNNNGC 7 cut(s) 117, 147, 201, 300, 718, 723, 998
HpyF3I CTNAG 5 cut(s) 815, 846, 927, 963, 993
Hsp92II CATG 7 cut(s) 54, 177, 263, 380, 619, 813, 1016
HspAI GCGC 2 cut(s) 111, 696
KroI GCCGGC 1 cut(s) 29
KroNI GCCGGC 1 cut(s) 31
Kzo9I GATC 4 cut(s) 220, 244, 515, 640
Lsp1109I GCAGC 3 cut(s) 104, 254, 262
LweI GCATC 2 cut(s) 175, 601
MaeI CTAG 7 cut(s) 224, 278, 396, 765, 788, 903, 1019
MaeIII GTNAC 1 cut(s) 780
MalI GATC 4 cut(s) 222, 246, 517, 642
MboI GATC 4 cut(s) 220, 244, 515, 640
MboII GAAGA 4 cut(s) 170, 179, 372, 891
MfeI CAATTG 1 cut(s) 647
MhlI GDGCHC 2 cut(s) 434, 845
MlsI TGGCCA 1 cut(s) 11
MluCI AATT 6 cut(s) 539, 573, 647, 741, 823, 920
MluNI TGGCCA 1 cut(s) 11
MlyI GAGTC 4 cut(s) 178, 385, 494, 694
MmeI TCCRAC 1 cut(s) 870
MnlI CCTC 7 cut(s) 152, 382, 616, 702, 798, 841, 928
Mox20I TGGCCA 1 cut(s) 11
MroNI GCCGGC 1 cut(s) 29
MscI TGGCCA 1 cut(s) 11
MseI TTAA 4 cut(s) 554, 735, 746, 822
MslI CAYNNNNRTG 2 cut(s) 381, 883
Msp20I TGGCCA 1 cut(s) 11
MspI CCGG 2 cut(s) 30, 653
MunI CAATTG 1 cut(s) 647
MwoI GCNNNNNNNGC 7 cut(s) 117, 147, 201, 300, 718, 723, 998
NaeI GCCGGC 1 cut(s) 31
NdeII GATC 4 cut(s) 220, 244, 515, 640
NgoMIV GCCGGC 1 cut(s) 29
NlaIII CATG 7 cut(s) 54, 177, 263, 380, 619, 813, 1016
NmeAIII GCCGAG 1 cut(s) 738
NmuCI GTSAC 1 cut(s) 780
NspI RCATGY 1 cut(s) 177
OliI CACNNNNGTG 1 cut(s) 883
PdiI GCCGGC 1 cut(s) 31
PfeI GAWTC 2 cut(s) 229, 635
PflFI GACNNNGTC 1 cut(s) 778
PkrI GCNGC 3 cut(s) 119, 252, 269
PleI GAGTC 4 cut(s) 178, 385, 493, 693
PpsI GAGTC 4 cut(s) 178, 385, 493, 693
PsiI TTATAA 2 cut(s) 444, 546
PsyI GACNNNGTC 1 cut(s) 778
RigI GGCCGGCC 1 cut(s) 33
RsaI GTAC 4 cut(s) 335, 534, 755, 896
RsaNI GTAC 4 cut(s) 334, 533, 754, 895
RseI CAYNNNNRTG 2 cut(s) 381, 883
SaqAI TTAA 4 cut(s) 554, 735, 746, 822
SatI GCNGC 3 cut(s) 118, 251, 268
Sau3AI GATC 4 cut(s) 220, 244, 515, 640
ScaI AGTACT 1 cut(s) 896
SchI GAGTC 4 cut(s) 178, 385, 494, 694
SduI GDGCHC 2 cut(s) 434, 845
SfaNI GCATC 2 cut(s) 175, 601
SmiMI CAYNNNNRTG 2 cut(s) 381, 883
Sse9I AATT 6 cut(s) 539, 573, 647, 741, 823, 920
SsiI CCGC 1 cut(s) 583
SspI AATATT 1 cut(s) 771
SspMI CTAG 7 cut(s) 224, 278, 396, 765, 788, 903, 1019
StyI CCWWGG 2 cut(s) 395, 425
TaaI ACNGT 1 cut(s) 93
TaqI TCGA 4 cut(s) 72, 169, 460, 609
TaqII GACCGA 1 cut(s) 77
TasI AATT 6 cut(s) 539, 573, 647, 741, 823, 920
TatI WGTACW 3 cut(s) 333, 532, 894
TfiI GAWTC 2 cut(s) 229, 635
Tru1I TTAA 4 cut(s) 554, 735, 746, 822
Tru9I TTAA 4 cut(s) 554, 735, 746, 822
TscAI CASTG 1 cut(s) 585
TseFI GTSAC 1 cut(s) 780
TseI GCWGC 3 cut(s) 117, 250, 267
Tsp45I GTSAC 1 cut(s) 780
TspDTI ATGAA 6 cut(s) 39, 58, 276, 393, 632, 903
TspGWI ACGGA 2 cut(s) 138, 201
TspRI CASTG 1 cut(s) 585
Tth111I GACNNNGTC 1 cut(s) 778
XapI RAATTY 3 cut(s) 539, 573, 741
XbaI TCTAGA 1 cut(s) 223
XceI RCATGY 1 cut(s) 177
XcmI CCANNNNNNNNNTGG 2 cut(s) 383, 700
XmaJI CCTAGG 1 cut(s) 395
XmiI GTMKAC 1 cut(s) 84
XspI CTAG 7 cut(s) 224, 278, 396, 765, 788, 903, 1019
ZrmI AGTACT 1 cut(s) 896
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.