Rw2G017740

DA1-related 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0008106
Physical Location & Seq
Forward (+)
22069022 .. 22070647
1626 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G017740.1

Sequence Viewer

Length: 1044 bp
ATGGCATCAAATACCCAAAGAGCCTCTACGTCAGAGACCGAGTTCATCGAAAAAGTTGTAGACAAACTGTATAACAAACTGGTATGCGCTGGCTGCAACGGAAGGGCTGGTGATGGCCTCAACTGCTTGGGTGCTGTTTGGCATCAAGCATGTCTTCTGACTCTTCTCTGCAAAGGTTGCAACCATCCGTTTACTGATCTAGAGATTCGGGAATGTGGGGATCAGCAGCCTTACCATGAACGCTGCTATGAGCTAGAATATCTTGTATGCAATGTTTGCAAGAAATCTATCCAAGATGGTCAAGTTGAGTACAAGGAATATCCATTCTGGCGACGAAGATATTGTTCCTCCCATGAAAATGATGGGACTCCTAGGTGTTGTAGCTGTGAGAGATTGAAGCCAAGGGGCACAACATATTATAAGCTTGATGATGGTCGAAAACTATGTCAGGAGTGTAAAGAGTCCATTATCAATACTGGTCAAGGACACCGATCCATTTGCCTTGAAGTACAAAAATTTTATAAGAAGTTAAATGTGAAAGTAGAGAAAATTCCAGTGCGGTTGGTCGGCAGACAAGTCATAATCGATGCCATGAAGGAGGGAAATGATGATTCTGATCGCCAATTACCGGTAATAAGAGCAGTCGGCTTGTTTGAAAAGAGTCTTACCAGCGCTATTATGGGGAGGCCGAGCCAGCTTTGCTGTGAAGTTAAAGAAATTTTAATCTGGTACGGCCTTCCTAGAATATTGACTGGGTCACTTCTAGCTCAACAGATGATGAGGGCATGGCTAAGGCTTAATTGCTGTCCGACTATGAGCCCTGAGGTTGAAAAAGGTATCTGCCGATATTTTGCACATAGGTGGTTGGAGTACTCTTCTAGTTCCTATTCATCTAAATTGTCTGAGTTTGAGGAGAAGCTTTGTAGGAAACGCCAAACTGAGTTAGGTTGTGAAGCATTACTGACAGCTAAGAAAGCTGTGTGCAAGCATGGTCTAGGAATGACACTTGAAACAATCCGAAATACAGGAGAGTTTCCATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0032182 GO:0043130 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DA1-like LIM

Protein Analysis

347

Amino Acids

39.67

Weight (kDa)

8.56

Isoelectric Point (pI)

40.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DA1-like PF12315 177 - 299 1.2e-24 Protein DA1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 420, 522
AccI GTMKAC 1 cut(s) 60
AciI CCGC 1 cut(s) 559
AclWI GGATC 2 cut(s) 228, 486
AcsI RAATTY 3 cut(s) 515, 549, 717
AfaI GTAC 4 cut(s) 311, 510, 731, 872
AfeI AGCGCT 1 cut(s) 673
AfiI CCNNNNNNNGG 1 cut(s) 628
AgeI ACCGGT 1 cut(s) 628
AgsI TTSAA 5 cut(s) 397, 506, 656, 830, 1010
AleI CACNNNNGTG 1 cut(s) 859
AluBI AGCT 8 cut(s) 253, 384, 424, 697, 767, 919, 968, 977
AluI AGCT 8 cut(s) 253, 384, 424, 697, 767, 919, 968, 977
Alw26I GTCTC 1 cut(s) 29
AlwI GGATC 2 cut(s) 228, 486
Aor51HI AGCGCT 1 cut(s) 673
AoxI GGCC 3 cut(s) 115, 686, 733
ApeKI GCWGC 3 cut(s) 93, 226, 243
ApoI RAATTY 3 cut(s) 515, 549, 717
ArsI GACNNNNNNTTYG 2 cut(s) 430, 462
AsiGI ACCGGT 1 cut(s) 628
AspA2I CCTAGG 1 cut(s) 371
AspLEI GCGC 2 cut(s) 89, 674
AsuHPI GGTGA 1 cut(s) 122
AvrII CCTAGG 1 cut(s) 371
AxyI CCTNAGG 1 cut(s) 822
BaeGI GKGCMC 1 cut(s) 410
BanII GRGCYC 1 cut(s) 821
BbsI GAAGAC 1 cut(s) 146
BbvI GCAGC 3 cut(s) 80, 230, 238
BccI CCATC 5 cut(s) 107, 192, 290, 356, 425
BceAI ACGGC 1 cut(s) 748
BcgI CGANNNNNNTGC 2 cut(s) 480, 514
BcoDI GTCTC 1 cut(s) 29
BfaI CTAG 7 cut(s) 200, 254, 372, 741, 764, 879, 995
BfoI RGCGCY 1 cut(s) 675
BisI GCNGC 3 cut(s) 94, 227, 244
BlnI CCTAGG 1 cut(s) 371
BlsI GCNGC 3 cut(s) 95, 228, 245
BmcAI AGTACT 1 cut(s) 872
BmrI ACTGGG 1 cut(s) 762
BmsI GCATC 3 cut(s) 14, 151, 577
BmuI ACTGGG 1 cut(s) 762
BpiI GAAGAC 1 cut(s) 146
Bpu10I CCTNAGC 1 cut(s) 791
Bsa29I ATCGAT 1 cut(s) 585
BsaBI GATNNNNATC 1 cut(s) 615
BsaI GGTCTC 1 cut(s) 29
BsaJI CCNNGG 2 cut(s) 371, 401
BsaWI WCCGGW 1 cut(s) 628
Bsc4I CCNNNNNNNGG 1 cut(s) 628
Bse118I RCCGGY 1 cut(s) 628
Bse1I ACTGG 4 cut(s) 84, 481, 554, 757
Bse21I CCTNAGG 1 cut(s) 822
Bse3DI GCAATG 1 cut(s) 277
Bse8I GATNNNNATC 1 cut(s) 615
BseCI ATCGAT 1 cut(s) 585
BseDI CCNNGG 2 cut(s) 371, 401
BseGI GGATG 1 cut(s) 184
BseJI GATNNNNATC 1 cut(s) 615
BseLI CCNNNNNNNGG 1 cut(s) 628
BseMI GCAATG 1 cut(s) 277
BseMII CTCAG 3 cut(s) 813, 894, 930
BseNI ACTGG 4 cut(s) 84, 481, 554, 757
BseRI GAGGAG 1 cut(s) 926
BseSI GKGCMC 1 cut(s) 410
BseXI GCAGC 3 cut(s) 80, 230, 238
BshFI GGCC 3 cut(s) 117, 688, 735
BshTI ACCGGT 1 cut(s) 628
BshVI ATCGAT 1 cut(s) 585
BsiSI CCGG 1 cut(s) 629
BslFI GGGAC 1 cut(s) 379
BslI CCNNNNNNNGG 1 cut(s) 628
BsmAI GTCTC 1 cut(s) 29
BsmFI GGGAC 1 cut(s) 379
BsnI GGCC 3 cut(s) 117, 688, 735
Bso31I GGTCTC 1 cut(s) 29
Bsp1286I GDGCHC 2 cut(s) 410, 821
Bsp143I GATC 4 cut(s) 196, 220, 491, 616
BspACI CCGC 1 cut(s) 559
BspANI GGCC 3 cut(s) 117, 688, 735
BspCNI CTCAG 3 cut(s) 814, 895, 931
BspDI ATCGAT 1 cut(s) 585
BspPI GGATC 2 cut(s) 228, 486
BspTNI GGTCTC 1 cut(s) 29
BsrDI GCAATG 1 cut(s) 277
BsrFI RCCGGY 1 cut(s) 628
BsrI ACTGG 4 cut(s) 84, 481, 554, 757
BssAI RCCGGY 1 cut(s) 628
BssECI CCNNGG 2 cut(s) 371, 401
BssMI GATC 4 cut(s) 196, 220, 491, 616
BssT1I CCWWGG 2 cut(s) 371, 401
Bst4CI ACNGT 1 cut(s) 69
Bst6I CTCTTC 2 cut(s) 168, 880
BstAPI GCANNNNNTGC 2 cut(s) 177, 276
BstC8I GCNNGC 3 cut(s) 91, 695, 986
BstDEI CTNAG 5 cut(s) 791, 822, 903, 939, 969
BstF5I GGATG 1 cut(s) 184
BstH2I RGCGCY 1 cut(s) 675
BstHHI GCGC 2 cut(s) 89, 674
BstKTI GATC 4 cut(s) 199, 223, 494, 619
BstMAI GTCTC 1 cut(s) 29
BstMBI GATC 4 cut(s) 196, 220, 491, 616
BstMWI GCNNNNNNNGC 7 cut(s) 93, 123, 177, 276, 694, 699, 974
BstNSI RCATGY 1 cut(s) 153
BstSLI GKGCMC 1 cut(s) 410
BstV1I GCAGC 3 cut(s) 80, 230, 238
BstV2I GAAGAC 1 cut(s) 146
Bsu15I ATCGAT 1 cut(s) 585
Bsu36I CCTNAGG 1 cut(s) 822
BsuRI GGCC 3 cut(s) 117, 688, 735
BsuTUI ATCGAT 1 cut(s) 585
BtsCI GGATG 1 cut(s) 184
BtsIMutI CAGTG 1 cut(s) 561
Cac8I GCNNGC 3 cut(s) 91, 695, 986
CfoI GCGC 2 cut(s) 89, 674
Cfr10I RCCGGY 1 cut(s) 628
ClaI ATCGAT 1 cut(s) 585
Csp6I GTAC 4 cut(s) 310, 509, 730, 871
CspAI ACCGGT 1 cut(s) 628
CviAII CATG 6 cut(s) 150, 236, 353, 592, 786, 989
CviQI GTAC 4 cut(s) 310, 509, 730, 871
DdeI CTNAG 5 cut(s) 791, 822, 903, 939, 969
DpnI GATC 4 cut(s) 198, 222, 493, 618
DpnII GATC 4 cut(s) 196, 220, 491, 616
Eam1104I CTCTTC 2 cut(s) 168, 880
EarI CTCTTC 2 cut(s) 168, 880
Eco130I CCWWGG 2 cut(s) 371, 401
Eco24I GRGCYC 1 cut(s) 821
Eco31I GGTCTC 1 cut(s) 29
Eco47III AGCGCT 1 cut(s) 673
Eco81I CCTNAGG 1 cut(s) 822
EcoT14I CCWWGG 2 cut(s) 371, 401
EcoT38I GRGCYC 1 cut(s) 821
ErhI CCWWGG 2 cut(s) 371, 401
FaeI CATG 6 cut(s) 153, 239, 356, 595, 789, 992
FalI AAGNNNNNCTT 2 cut(s) 138, 170
FaqI GGGAC 1 cut(s) 379
FatI CATG 6 cut(s) 149, 235, 352, 591, 785, 988
FblI GTMKAC 1 cut(s) 60
Fnu4HI GCNGC 3 cut(s) 94, 227, 244
FokI GGATG 1 cut(s) 171
FriOI GRGCYC 1 cut(s) 821
Fsp4HI GCNGC 3 cut(s) 94, 227, 244
FspBI CTAG 7 cut(s) 200, 254, 372, 741, 764, 879, 995
GlaI GCGC 2 cut(s) 88, 673
GluI GCNGC 3 cut(s) 94, 227, 244
HaeII RGCGCY 1 cut(s) 675
HaeIII GGCC 3 cut(s) 117, 688, 735
HapII CCGG 1 cut(s) 629
HhaI GCGC 2 cut(s) 89, 674
Hin1II CATG 6 cut(s) 153, 239, 356, 595, 789, 992
Hin6I GCGC 2 cut(s) 87, 672
HinP1I GCGC 2 cut(s) 87, 672
HindIII AAGCTT 2 cut(s) 422, 917
HinfI GANTC 6 cut(s) 160, 205, 367, 461, 611, 661
HpaII CCGG 1 cut(s) 629
HphI GGTGA 1 cut(s) 122
Hpy166II GTNNAC 2 cut(s) 61, 192
Hpy188I TCNGA 6 cut(s) 34, 159, 616, 810, 904, 1019
Hpy188III TCNNGA 3 cut(s) 200, 209, 449
Hpy8I GTNNAC 2 cut(s) 61, 192
Hpy99I CGWCG 1 cut(s) 336
HpyAV CCTTC 3 cut(s) 96, 589, 746
HpyCH4III ACNGT 1 cut(s) 69
HpyCH4IV ACGT 1 cut(s) 29
HpyCH4V TGCA 7 cut(s) 96, 171, 180, 270, 279, 854, 984
HpyF10VI GCNNNNNNNGC 7 cut(s) 93, 123, 177, 276, 694, 699, 974
HpyF3I CTNAG 5 cut(s) 791, 822, 903, 939, 969
HpySE526I ACGT 1 cut(s) 29
Hsp92II CATG 6 cut(s) 153, 239, 356, 595, 789, 992
HspAI GCGC 2 cut(s) 87, 672
Kzo9I GATC 4 cut(s) 196, 220, 491, 616
Lsp1109I GCAGC 3 cut(s) 80, 230, 238
LweI GCATC 3 cut(s) 14, 151, 577
MaeI CTAG 7 cut(s) 200, 254, 372, 741, 764, 879, 995
MaeII ACGT 1 cut(s) 29
MaeIII GTNAC 1 cut(s) 756
MalI GATC 4 cut(s) 198, 222, 493, 618
MboI GATC 4 cut(s) 196, 220, 491, 616
MboII GAAGA 4 cut(s) 146, 155, 348, 867
MhlI GDGCHC 2 cut(s) 410, 821
MluCI AATT 6 cut(s) 515, 549, 623, 717, 799, 896
MlyI GAGTC 4 cut(s) 154, 361, 470, 670
MmeI TCCRAC 2 cut(s) 833, 846
MnlI CCTC 8 cut(s) 34, 128, 358, 592, 678, 774, 817, 904
MseI TTAA 4 cut(s) 530, 711, 722, 798
MslI CAYNNNNRTG 2 cut(s) 357, 859
MspI CCGG 1 cut(s) 629
MwoI GCNNNNNNNGC 7 cut(s) 93, 123, 177, 276, 694, 699, 974
NdeII GATC 4 cut(s) 196, 220, 491, 616
NlaIII CATG 6 cut(s) 153, 239, 356, 595, 789, 992
NmeAIII GCCGAG 1 cut(s) 714
NmuCI GTSAC 1 cut(s) 756
NspI RCATGY 1 cut(s) 153
OliI CACNNNNGTG 1 cut(s) 859
PfeI GAWTC 2 cut(s) 205, 611
PflFI GACNNNGTC 1 cut(s) 754
PinAI ACCGGT 1 cut(s) 628
PkrI GCNGC 3 cut(s) 95, 228, 245
PleI GAGTC 4 cut(s) 154, 361, 469, 669
PpsI GAGTC 4 cut(s) 154, 361, 469, 669
PsiI TTATAA 2 cut(s) 420, 522
PsyI GACNNNGTC 1 cut(s) 754
RsaI GTAC 4 cut(s) 311, 510, 731, 872
RsaNI GTAC 4 cut(s) 310, 509, 730, 871
RseI CAYNNNNRTG 2 cut(s) 357, 859
SaqAI TTAA 4 cut(s) 530, 711, 722, 798
SatI GCNGC 3 cut(s) 94, 227, 244
Sau3AI GATC 4 cut(s) 196, 220, 491, 616
ScaI AGTACT 1 cut(s) 872
SchI GAGTC 4 cut(s) 154, 361, 470, 670
SduI GDGCHC 2 cut(s) 410, 821
SfaNI GCATC 3 cut(s) 14, 151, 577
SmiMI CAYNNNNRTG 2 cut(s) 357, 859
Sse9I AATT 6 cut(s) 515, 549, 623, 717, 799, 896
SsiI CCGC 1 cut(s) 559
SspI AATATT 1 cut(s) 747
SspMI CTAG 7 cut(s) 200, 254, 372, 741, 764, 879, 995
StyI CCWWGG 2 cut(s) 371, 401
TaaI ACNGT 1 cut(s) 69
TaiI ACGT 1 cut(s) 32
TaqI TCGA 3 cut(s) 48, 436, 585
TaqII GACCGA 1 cut(s) 53
TasI AATT 6 cut(s) 515, 549, 623, 717, 799, 896
TatI WGTACW 3 cut(s) 309, 508, 870
TfiI GAWTC 2 cut(s) 205, 611
Tru1I TTAA 4 cut(s) 530, 711, 722, 798
Tru9I TTAA 4 cut(s) 530, 711, 722, 798
TscAI CASTG 1 cut(s) 561
TseFI GTSAC 1 cut(s) 756
TseI GCWGC 3 cut(s) 93, 226, 243
Tsp45I GTSAC 1 cut(s) 756
TspDTI ATGAA 5 cut(s) 34, 252, 369, 608, 879
TspGWI ACGGA 2 cut(s) 114, 177
TspRI CASTG 1 cut(s) 561
Tth111I GACNNNGTC 1 cut(s) 754
XapI RAATTY 3 cut(s) 515, 549, 717
XbaI TCTAGA 1 cut(s) 199
XceI RCATGY 1 cut(s) 153
XcmI CCANNNNNNNNNTGG 2 cut(s) 359, 676
XmaJI CCTAGG 1 cut(s) 371
XmiI GTMKAC 1 cut(s) 60
XspI CTAG 7 cut(s) 200, 254, 372, 741, 764, 879, 995
ZrmI AGTACT 1 cut(s) 872
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.