Rh2AG229200

DA1-related 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0008106
Physical Location & Seq
Forward (+)
24382021 .. 24384310
2290 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG229200.1

Sequence Viewer

Length: 1533 bp
ATGGCATCAAGCACCCAAAGAGCCTCTACGTCCATTCCTTCTGCTTCTTTGTGGAAATATGATGCGTTTTTGAGCTTTAGGGGGAAAGAAACCCGCAAGACTTTTACAGACCATCTACATAAAGCATTGGCGGGGAAAAGAATCAAAGTTTTCAAGGATGAAAAACTTGAGAGAGGCACAACTATCTCTTCAGAGCTCTTGACTGCAATCTCAAAATCAAGGTTTGCCATTATTGTCATCTCACAAGATTACGCCAGTTCAAAATGGTGCTTAGATGAACTCGTAGAGATTTTTGAATGCATGAAGGACTGGAAGACAATTATCCCGATTTTTTATGATGTGGATCCATCCGATGTAAGAAACCAAAGAGGAACTTTTAAAGATGCCTTCAATAAGCATGAAAAAGACGGTATACATGACACAGAAAAGTTGCGAGGGTGGAAGAAGGCTTTGACAGCAGTTGGTGGCCTATCTGGGTGGATTTGGGGACAATATCGGTATGAAACCAAGTTTATCGAAGAAATTGTAGACTCATTGTATAACAAACTGGTGTGCGCTGGCTGCAACAGGAGGGTTGAAGATGATGGCCTCAACTGCTTGGATGCTCTTTGGCATCGAGCATGTCTTCTCTGCAAAGGCTGCAATCGTCCGTTTACTGATCTTCAATTTTGGGAATGTGGGAATCAGCCTTACCATGAACGCTGCTATGAGCTACAATGTCAAGTATGCAATGTTTGCAAGAAATCTATCCAAGATAATTCAGATGGTCAAGTTGAGTATATGGAACATCCATTCTGGCGACAAAGATATTGTCCCTCCCATGTAAAGGATGGGACGCCTTGGTGTTGTAGCTGTGAAAGATTGAAGCCAAGGGACATAACATATTATAAGCTTGATGATGGTCGAAAACTATGTCCGGAATGTAAAGAGTCTATTATCAATACTGATAGCGGACGCCGATCCATTTGCCTTGAAGTACGAAAATTTTATAAGAAATTAAATGTGAAAGTAGAGAAGATTCCAGTGCGGTTGGTCAGCAGTCAAGACATAATCGATGTCTTGAAGGAGGCAAATGATGGTTCTGATCCCCAATTGCCGGTAATAAGAGCTGTCTGCTTGTGTGAAGAGCGTACTGTTACCAGCACTATTTGGGGGAGGCCGAGCCAGCTTTGCTGTGAAGTTAAAGAAATTTTAATCTGGTACGGCTTTCCAAGAGTACTGACAGGGTCACTTCTAGCTCAACAGATGATGAGTGCATGGCTAAGGCTTAATCGTTATCCAAATTTGCGCCCTGAGGTTGAAAAAGGTATCTGCCGATATTTTGAAAATAGGTGGTTGGACTACTCTTCTAATTCTGATTCATCTATAAATTGCTCTGAGTTTGAGAAGAAGCTTTGTAAATTTCGGAAACGCAAAATTGAATCAGACTGTGAAGCATTACTGACAGCAAAGGAAGCGGTGCGCAAGCATGGTCTAGGAATGACACTAGAAACAATTCGGAAGACAGGAAAGTTTCCATCTGTAACGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0032182 GO:0043130 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DA1-like LIM

Protein Analysis

510

Amino Acids

59.11

Weight (kDa)

8.73

Isoelectric Point (pI)

44.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 19 - 183 9.3e-50 TIR domain
TIR_2 PF13676 23 - 128 1.6e-13 TIR domain
LIM PF00412 185 - 237 1.1e-07 LIM domain
DA1-like PF12315 333 - 501 5e-26 Protein DA1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 888, 990
Acc16I TGCGCA 1 cut(s) 1463
AccI GTMKAC 2 cut(s) 412, 528
AccIII TCCGGA 1 cut(s) 916
AciI CCGC 5 cut(s) 94, 131, 951, 1027, 1457
AclWI GGATC 4 cut(s) 338, 351, 954, 1079
AcsI RAATTY 4 cut(s) 983, 1188, 1282, 1400
AcuI CTGAAG 1 cut(s) 174
AcyI GRCGYC 2 cut(s) 836, 955
AfaI GTAC 4 cut(s) 978, 1132, 1202, 1218
AfiI CCNNNNNNNGG 2 cut(s) 826, 1096
AluBI AGCT 9 cut(s) 75, 196, 712, 852, 892, 1109, 1168, 1238, 1393
AluI AGCT 9 cut(s) 75, 196, 712, 852, 892, 1109, 1168, 1238, 1393
Alw21I GWGCWC 1 cut(s) 198
AlwI GGATC 4 cut(s) 338, 351, 954, 1079
Aor13HI TCCGGA 1 cut(s) 916
AoxI GGCC 3 cut(s) 466, 586, 1157
ApeKI GCWGC 3 cut(s) 561, 639, 702
ApoI RAATTY 4 cut(s) 983, 1188, 1282, 1400
ArsI GACNNNNNNTTYG 4 cut(s) 796, 828, 898, 930
Asp700I GAANNNNTTC 1 cut(s) 1494
AspLEI GCGC 3 cut(s) 557, 1290, 1464
AxyI CCTNAGG 1 cut(s) 1293
BamHI GGATCC 1 cut(s) 343
BanII GRGCYC 1 cut(s) 198
BbsI GAAGAC 3 cut(s) 320, 617, 1508
Bbv12I GWGCWC 1 cut(s) 198
BbvI GCAGC 3 cut(s) 548, 626, 689
BccI CCATC 8 cut(s) 120, 355, 578, 758, 824, 893, 1070, 1525
BceAI ACGGC 1 cut(s) 1219
BcgI CGANNNNNNTGC 2 cut(s) 948, 982
BfaI CTAG 3 cut(s) 1235, 1475, 1487
BisI GCNGC 3 cut(s) 562, 640, 703
BlsI GCNGC 3 cut(s) 563, 641, 704
BmcAI AGTACT 1 cut(s) 1218
BmiI GGNNCC 1 cut(s) 345
BmsI GCATC 5 cut(s) 14, 52, 373, 592, 622
BpiI GAAGAC 3 cut(s) 320, 617, 1508
Bpu10I CCTNAGC 1 cut(s) 1262
BpuEI CTTGAG 1 cut(s) 188
Bsa29I ATCGAT 1 cut(s) 1053
BsaBI GATNNNNATC 1 cut(s) 342
BsaHI GRCGYC 2 cut(s) 836, 955
BsaJI CCNNGG 2 cut(s) 839, 869
BsaWI WCCGGW 1 cut(s) 916
Bsc4I CCNNNNNNNGG 2 cut(s) 826, 1096
Bse118I RCCGGY 1 cut(s) 1096
Bse1I ACTGG 4 cut(s) 255, 314, 552, 1022
Bse21I CCTNAGG 1 cut(s) 1293
Bse3DI GCAATG 1 cut(s) 736
Bse8I GATNNNNATC 1 cut(s) 342
BseAI TCCGGA 1 cut(s) 916
BseCI ATCGAT 1 cut(s) 1053
BseDI CCNNGG 2 cut(s) 839, 869
BseGI GGATG 5 cut(s) 163, 347, 607, 787, 835
BseJI GATNNNNATC 1 cut(s) 342
BseLI CCNNNNNNNGG 2 cut(s) 826, 1096
BseMI GCAATG 1 cut(s) 736
BseMII CTCAG 2 cut(s) 1284, 1368
BseNI ACTGG 4 cut(s) 255, 314, 552, 1022
BseXI GCAGC 3 cut(s) 548, 626, 689
BshFI GGCC 3 cut(s) 468, 588, 1159
BshVI ATCGAT 1 cut(s) 1053
BsiHKAI GWGCWC 1 cut(s) 198
BsiSI CCGG 2 cut(s) 917, 1097
BslFI GGGAC 4 cut(s) 501, 798, 847, 887
BslI CCNNNNNNNGG 2 cut(s) 826, 1096
BsmFI GGGAC 4 cut(s) 501, 798, 847, 887
BsmI GAATGC 1 cut(s) 302
BsnI GGCC 3 cut(s) 468, 588, 1159
Bsp1286I GDGCHC 1 cut(s) 198
Bsp13I TCCGGA 1 cut(s) 916
Bsp143I GATC 4 cut(s) 343, 658, 959, 1084
BspACI CCGC 5 cut(s) 94, 131, 951, 1027, 1457
BspANI GGCC 3 cut(s) 468, 588, 1159
BspCNI CTCAG 2 cut(s) 1285, 1369
BspDI ATCGAT 1 cut(s) 1053
BspEI TCCGGA 1 cut(s) 916
BspLI GGNNCC 1 cut(s) 345
BspPI GGATC 4 cut(s) 338, 351, 954, 1079
BspQI GCTCTTC 1 cut(s) 1119
BsrDI GCAATG 1 cut(s) 736
BsrFI RCCGGY 1 cut(s) 1096
BsrI ACTGG 4 cut(s) 255, 314, 552, 1022
BssAI RCCGGY 1 cut(s) 1096
BssECI CCNNGG 2 cut(s) 839, 869
BssMI GATC 4 cut(s) 343, 658, 959, 1084
BssNAI GTATAC 1 cut(s) 413
BssNI GRCGYC 2 cut(s) 836, 955
BssT1I CCWWGG 2 cut(s) 839, 869
Bst1107I GTATAC 1 cut(s) 413
Bst4CI ACNGT 3 cut(s) 410, 1135, 1430
Bst6I CTCTTC 3 cut(s) 193, 1119, 1351
BstACI GRCGYC 2 cut(s) 836, 955
BstAPI GCANNNNNTGC 2 cut(s) 639, 735
BstC8I GCNNGC 3 cut(s) 559, 1166, 1466
BstDEI CTNAG 4 cut(s) 271, 1262, 1293, 1377
BstF5I GGATG 5 cut(s) 163, 347, 607, 787, 835
BstHHI GCGC 3 cut(s) 557, 1290, 1464
BstKTI GATC 4 cut(s) 346, 661, 962, 1087
BstMBI GATC 4 cut(s) 343, 658, 959, 1084
BstMWI GCNNNNNNNGC 8 cut(s) 455, 561, 594, 639, 735, 1165, 1170, 1454
BstNSI RCATGY 1 cut(s) 624
BstV1I GCAGC 3 cut(s) 548, 626, 689
BstV2I GAAGAC 3 cut(s) 320, 617, 1508
BstX2I RGATCY 1 cut(s) 343
BstYI RGATCY 1 cut(s) 343
BstZ17I GTATAC 1 cut(s) 413
Bsu15I ATCGAT 1 cut(s) 1053
Bsu36I CCTNAGG 1 cut(s) 1293
BsuRI GGCC 3 cut(s) 468, 588, 1159
BsuTUI ATCGAT 1 cut(s) 1053
BtsCI GGATG 5 cut(s) 163, 347, 607, 787, 835
BtsIMutI CAGTG 2 cut(s) 1029, 1528
Cac8I GCNNGC 3 cut(s) 559, 1166, 1466
CfoI GCGC 3 cut(s) 557, 1290, 1464
Cfr10I RCCGGY 1 cut(s) 1096
ClaI ATCGAT 1 cut(s) 1053
CseI GACGC 2 cut(s) 844, 963
Csp6I GTAC 4 cut(s) 977, 1131, 1201, 1217
CviAII CATG 8 cut(s) 301, 398, 416, 621, 695, 821, 1257, 1469
CviQI GTAC 4 cut(s) 977, 1131, 1201, 1217
DdeI CTNAG 4 cut(s) 271, 1262, 1293, 1377
DpnI GATC 4 cut(s) 345, 660, 961, 1086
DpnII GATC 4 cut(s) 343, 658, 959, 1084
DraI TTTAAA 1 cut(s) 379
Eam1104I CTCTTC 3 cut(s) 193, 1119, 1351
EarI CTCTTC 3 cut(s) 193, 1119, 1351
Ecl136II GAGCTC 1 cut(s) 196
Eco130I CCWWGG 2 cut(s) 839, 869
Eco24I GRGCYC 1 cut(s) 198
Eco53kI GAGCTC 1 cut(s) 196
Eco57I CTGAAG 1 cut(s) 174
Eco81I CCTNAGG 1 cut(s) 1293
EcoICRI GAGCTC 1 cut(s) 196
EcoT14I CCWWGG 2 cut(s) 839, 869
EcoT22I ATGCAT 1 cut(s) 302
EcoT38I GRGCYC 1 cut(s) 198
ErhI CCWWGG 2 cut(s) 839, 869
FaeI CATG 8 cut(s) 304, 401, 419, 624, 698, 824, 1260, 1472
FalI AAGNNNNNCTT 2 cut(s) 358, 390
FaqI GGGAC 4 cut(s) 501, 798, 847, 887
FatI CATG 8 cut(s) 300, 397, 415, 620, 694, 820, 1256, 1468
FauI CCCGC 2 cut(s) 101, 124
FblI GTMKAC 2 cut(s) 412, 528
Fnu4HI GCNGC 3 cut(s) 562, 640, 703
FokI GGATG 5 cut(s) 170, 334, 614, 774, 842
FriOI GRGCYC 1 cut(s) 198
Fsp4HI GCNGC 3 cut(s) 562, 640, 703
FspBI CTAG 3 cut(s) 1235, 1475, 1487
FspI TGCGCA 1 cut(s) 1463
GlaI GCGC 3 cut(s) 556, 1289, 1463
GluI GCNGC 3 cut(s) 562, 640, 703
HaeIII GGCC 3 cut(s) 468, 588, 1159
HapII CCGG 2 cut(s) 917, 1097
HgaI GACGC 2 cut(s) 844, 963
HhaI GCGC 3 cut(s) 557, 1290, 1464
Hin1I GRCGYC 2 cut(s) 836, 955
Hin1II CATG 8 cut(s) 304, 401, 419, 624, 698, 824, 1260, 1472
Hin6I GCGC 3 cut(s) 555, 1288, 1462
HinP1I GCGC 3 cut(s) 555, 1288, 1462
HindIII AAGCTT 2 cut(s) 890, 1391
HinfI GANTC 7 cut(s) 141, 530, 682, 929, 1018, 1358, 1421
HpaII CCGG 2 cut(s) 917, 1097
Hpy166II GTNNAC 3 cut(s) 413, 529, 654
Hpy188I TCNGA 9 cut(s) 193, 352, 763, 1084, 1357, 1378, 1407, 1426, 1500
Hpy188III TCNNGA 5 cut(s) 199, 325, 917, 1043, 1060
Hpy8I GTNNAC 3 cut(s) 413, 529, 654
HpyAV CCTTC 5 cut(s) 48, 298, 397, 439, 1057
HpyCH4III ACNGT 3 cut(s) 410, 1135, 1430
HpyCH4IV ACGT 1 cut(s) 29
HpyCH4V TGCA 8 cut(s) 206, 300, 564, 633, 642, 729, 738, 1256
HpyF10VI GCNNNNNNNGC 8 cut(s) 455, 561, 594, 639, 735, 1165, 1170, 1454
HpyF3I CTNAG 4 cut(s) 271, 1262, 1293, 1377
HpySE526I ACGT 1 cut(s) 29
Hsp92I GRCGYC 2 cut(s) 836, 955
Hsp92II CATG 8 cut(s) 304, 401, 419, 624, 698, 824, 1260, 1472
HspAI GCGC 3 cut(s) 555, 1288, 1462
Kpn2I TCCGGA 1 cut(s) 916
Kzo9I GATC 4 cut(s) 343, 658, 959, 1084
LguI GCTCTTC 1 cut(s) 1119
Lsp1109I GCAGC 3 cut(s) 548, 626, 689
LweI GCATC 5 cut(s) 14, 52, 373, 592, 622
MaeI CTAG 3 cut(s) 1235, 1475, 1487
MaeII ACGT 1 cut(s) 29
MaeIII GTNAC 3 cut(s) 1135, 1227, 1522
MalI GATC 4 cut(s) 345, 660, 961, 1086
MboI GATC 4 cut(s) 343, 658, 959, 1084
MfeI CAATTG 1 cut(s) 1091
MflI RGATCY 1 cut(s) 343
MhlI GDGCHC 1 cut(s) 198
MlyI GAGTC 2 cut(s) 524, 938
MmeI TCCRAC 1 cut(s) 1317
Mph1103I ATGCAT 1 cut(s) 302
MroI TCCGGA 1 cut(s) 916
MroXI GAANNNNTTC 1 cut(s) 1494
MseI TTAA 5 cut(s) 378, 998, 1182, 1193, 1269
MspI CCGG 2 cut(s) 917, 1097
MunI CAATTG 1 cut(s) 1091
Mva1269I GAATGC 1 cut(s) 302
MwoI GCNNNNNNNGC 8 cut(s) 455, 561, 594, 639, 735, 1165, 1170, 1454
NdeII GATC 4 cut(s) 343, 658, 959, 1084
NlaIII CATG 8 cut(s) 304, 401, 419, 624, 698, 824, 1260, 1472
NlaIV GGNNCC 1 cut(s) 345
NmeAIII GCCGAG 1 cut(s) 1185
NmuCI GTSAC 1 cut(s) 1227
NsbI TGCGCA 1 cut(s) 1463
NsiI ATGCAT 1 cut(s) 302
NspI RCATGY 1 cut(s) 624
PciSI GCTCTTC 1 cut(s) 1119
PctI GAATGC 1 cut(s) 302
PdmI GAANNNNTTC 1 cut(s) 1494
PfeI GAWTC 5 cut(s) 141, 682, 1018, 1358, 1421
PflFI GACNNNGTC 1 cut(s) 1225
PkrI GCNGC 3 cut(s) 563, 641, 704
PleI GAGTC 2 cut(s) 524, 937
PpsI GAGTC 2 cut(s) 524, 937
PsiI TTATAA 2 cut(s) 888, 990
Psp124BI GAGCTC 1 cut(s) 198
PspN4I GGNNCC 1 cut(s) 345
PsuI RGATCY 1 cut(s) 343
PsyI GACNNNGTC 1 cut(s) 1225
RsaI GTAC 4 cut(s) 978, 1132, 1202, 1218
RsaNI GTAC 4 cut(s) 977, 1131, 1201, 1217
SacI GAGCTC 1 cut(s) 198
SapI GCTCTTC 1 cut(s) 1119
SaqAI TTAA 5 cut(s) 378, 998, 1182, 1193, 1269
SatI GCNGC 3 cut(s) 562, 640, 703
Sau3AI GATC 4 cut(s) 343, 658, 959, 1084
ScaI AGTACT 1 cut(s) 1218
SchI GAGTC 2 cut(s) 524, 938
SduI GDGCHC 1 cut(s) 198
SfaNI GCATC 5 cut(s) 14, 52, 373, 592, 622
SmlI CTYRAG 1 cut(s) 167
SmoI CTYRAG 1 cut(s) 167
SsiI CCGC 5 cut(s) 94, 131, 951, 1027, 1457
SspMI CTAG 3 cut(s) 1235, 1475, 1487
SstI GAGCTC 1 cut(s) 198
StyI CCWWGG 2 cut(s) 839, 869
TaaI ACNGT 3 cut(s) 410, 1135, 1430
TaiI ACGT 1 cut(s) 32
TaqI TCGA 4 cut(s) 516, 616, 904, 1053
TatI WGTACW 1 cut(s) 1216
TfiI GAWTC 5 cut(s) 141, 682, 1018, 1358, 1421
Tru1I TTAA 5 cut(s) 378, 998, 1182, 1193, 1269
Tru9I TTAA 5 cut(s) 378, 998, 1182, 1193, 1269
TscAI CASTG 1 cut(s) 1029
TseFI GTSAC 1 cut(s) 1227
TseI GCWGC 3 cut(s) 561, 639, 702
Tsp45I GTSAC 1 cut(s) 1227
TspDTI ATGAA 7 cut(s) 174, 291, 317, 414, 516, 711, 1350
TspGWI ACGGA 1 cut(s) 639
TspRI CASTG 1 cut(s) 1029
Tth111I GACNNNGTC 1 cut(s) 1225
XapI RAATTY 4 cut(s) 983, 1188, 1282, 1400
XceI RCATGY 1 cut(s) 624
XcmI CCANNNNNNNNNTGG 1 cut(s) 827
XmiI GTMKAC 2 cut(s) 412, 528
XmnI GAANNNNTTC 1 cut(s) 1494
XspI CTAG 3 cut(s) 1235, 1475, 1487
ZrmI AGTACT 1 cut(s) 1218
Zsp2I ATGCAT 1 cut(s) 302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.