Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0003144

Physical Location & Seq

Genomic Coordinates Reverse (-)

8528426 .. 8534420

Gene Structure 5995 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1062800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1062 bp

ATGGAGCTCTGTGGAGCCACTGCTCTCTCATGGACCCACACTGTCTCCACCCCTCCTCACGTCTCTCTGCCCCTCTCTAAGTGTGGGACTCTGAGGCTCAGTGTTTGGTGTGCCTCTGATCAAACCCAGACGGCAAATCAGATGACTGTATCAATAACTGGAGCTACGGGTTTTATCGGGAGAAGATTGGTGCAAAGGCTGCATGCAGATAATCATAGTGTACGTGTCTTGACACGGTCCAGATCAAAGGCTGAGTTAATTTTTCCGGTCAAGGACTTTCCAGGAATCATGATTGCAGAGGAGGCACAGTGGAAAGACAGCATTCAAGGTTCAACCGGTGTCGTAAATTTGGCTGGACTGCCCATAAGCACAAGATGGTCTCCTGAGATAAAGAAAGAGATCAAGACTAGCAGGATAAGGGTCACCTCAAAGGTCATAGATTTAATAAACGATTTACCAGATGCAGTTCGACCTACAGTCTTGGTTAGCGCAACAGCTGTCGGTTACTATGGTACTAGTGAGACACAAGTATTTGATGAGCAAAGTCCGTCAGGGAATGATTACTTAGCTGAGGTTTGTAGAGAATGGGAAGGAACTGCCCGCAAAGTGAATAAGGATGTTAGGCTAGCACTCATTCGCATTGGTGTTGTCCTTGGTAAAGATGGCGGAGCTTTAGCTAAAATGATCCCTCTCTTTATGGTGTTTGCTGGAGGACCGTTGGGCTCTGGAAAGCAATGGTTTTCCTGGATTCATCTGGATGACATAGTGAACCTGATATATGAAGCCCTATCCAATCCATCTTATGAAGGAGTTATAAATGGAACTGCACCCAACCCTGTTCGATTTGCAGAAATGTGTGAACATTTGGGAAATGTCTTGGGCAGGCCCTCATGGCTGCCTGTACCGGACTTTGCCCTGAAAGCAGTCCTTGGCGAAGGTGCTTCTGTGGTTCTGGATGGGCAGAGGGTGCTTCCAGTTAGAGCCAAGGAGCTGGGTTTCTCGTTCAAATACTCGTATGTGAGAGATGCATTACAATCCATTATTTCACAAAAGGCGGCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006996 GO:0008150 GO:0009507 GO:0009526 GO:0009536 GO:0009657 GO:0009658 GO:0009941 GO:0009987 GO:0010020 GO:0016043 GO:0031967 GO:0031975 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043572 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046983 GO:0048285 GO:0071840

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF1731 Epimerase

Protein Analysis

354

Amino Acids

38.42

Weight (kDa)

8.74

Isoelectric Point (pI)

31.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Epimerase	PF01370	51 - 267	1.1e-19	NAD dependent epimerase/dehydratase family
DUF1731	PF08338	301 - 347	3.1e-17	Domain of unknown function (DUF1731)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003144)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G21280 AT2G21280
fragaria_vesca	FvH4_2g10480
malus_domestica	MD05G1055500.v1.1 MD10G1062800.v1.1
prunus_persica	Prupe.8G083600_v2.0.a1 Prupe.8G083600_v2.0.a1 Prupe.8G083600_v2.0.a1
pyrus_communis	pycom05g04580 pycom10g04850
rosa_chinensis	RchiOBHm_Chr6g0267981
rosa_laevigata	RLG00000013948 RLG00000013951 RLG00000013955 RLG00000013960 RLG00000013964 RLG00000013989
rosa_multiflora	Rmu_sc0004529.1_g000001 Rmu_sc0008552.1_g000009 Rmu_sc0014221.1_g000002
rosa_roxburghii	Rroxscaffold_3G00234730 Rroxscaffold_7G00200240 Rroxscaffold_7G00200260
rosa_rugosa	Rorug06G0036300 Rorug06G0036400 Rorug06G0036500 Rorug06G0036600
rosa_samantha	Rh6BG160000 Rh6BG160900 Rh6BG161600 Rh6CG156900 Rh6DG145700 Rh6DG146400 Rh6DG147100
rosa_wichuraiana	Rw0G019640 Rw0G021320 Rw6G013740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	815
AciI	CCGC	3 cut(s)	601, 666, 1055
AclWI	GGATC	1 cut(s)	679
AcsI	RAATTY	1 cut(s)	346
AfaI	GTAC	3 cut(s)	222, 514, 903
AflIII	ACRYGT	1 cut(s)	223
AgeI	ACCGGT	1 cut(s)	335
AgsI	TTSAA	3 cut(s)	326, 333, 1006
AhdI	GACNNNNNGTC	1 cut(s)	476
AhlI	ACTAGT	1 cut(s)	515
AjiI	CACGTC	1 cut(s)	61
AjnI	CCWGG	2 cut(s)	280, 743
AluBI	AGCT	7 cut(s)	7, 164, 497, 569, 671, 677, 991
AluI	AGCT	7 cut(s)	7, 164, 497, 569, 671, 677, 991
Alw21I	GWGCWC	1 cut(s)	9
Alw26I	GTCTC	4 cut(s)	49, 67, 384, 515
AlwI	GGATC	1 cut(s)	679
AoxI	GGCC	2 cut(s)	884, 1056
ApeKI	GCWGC	2 cut(s)	199, 895
ApoI	RAATTY	1 cut(s)	346
AsiGI	ACCGGT	1 cut(s)	335
AspLEI	GCGC	1 cut(s)	491
AspS9I	GGNCC	4 cut(s)	33, 237, 713, 885
AsuHPI	GGTGA	1 cut(s)	415
AsuNHI	GCTAGC	1 cut(s)	625
AvaII	GGWCC	3 cut(s)	33, 237, 713
BanII	GRGCYC	2 cut(s)	9, 725
Bbv12I	GWGCWC	1 cut(s)	9
BbvCI	CCTCAGC	1 cut(s)	570
BbvI	GCAGC	2 cut(s)	186, 882
BccI	CCATC	4 cut(s)	369, 656, 805, 950
BceAI	ACGGC	1 cut(s)	147
BciT130I	CCWGG	2 cut(s)	282, 745
BclI	TGATCA	1 cut(s)	118
BcoDI	GTCTC	4 cut(s)	49, 67, 384, 515
BcuI	ACTAGT	1 cut(s)	515
BfaI	CTAG	3 cut(s)	408, 516, 626
BfmI	CTRYAG	1 cut(s)	474
BglI	GCCNNNNNGGC	1 cut(s)	892
BisI	GCNGC	3 cut(s)	200, 896, 1056
BlsI	GCNGC	3 cut(s)	201, 897, 1057
Bme1390I	CCNGG	2 cut(s)	282, 745
Bme18I	GGWCC	3 cut(s)	33, 237, 713
BmeRI	GACNNNNNGTC	1 cut(s)	476
BmgBI	CACGTC	1 cut(s)	61
BmgT120I	GGNCC	4 cut(s)	33, 237, 713, 885
BmiI	GGNNCC	2 cut(s)	16, 35
BmrFI	CCNGG	2 cut(s)	282, 745
BmsI	GCATC	2 cut(s)	451, 1015
BmtI	GCTAGC	1 cut(s)	629
BpmI	CTGGAG	2 cut(s)	180, 729
Bpu10I	CCTNAGC	1 cut(s)	570
BsaAI	YACGTR	1 cut(s)	224
BsaI	GGTCTC	1 cut(s)	384
BsaJI	CCNNGG	3 cut(s)	652, 928, 984
BsaWI	WCCGGW	3 cut(s)	265, 335, 904
BsaXI	ACNNNNNCTCC	4 cut(s)	29, 59, 293, 323
Bse118I	RCCGGY	1 cut(s)	335
Bse1I	ACTGG	2 cut(s)	163, 974
Bse3DI	GCAATG	1 cut(s)	740
BseBI	CCWGG	2 cut(s)	282, 745
BseDI	CCNNGG	3 cut(s)	652, 928, 984
BseGI	GGATG	3 cut(s)	622, 763, 961
BseMI	GCAATG	1 cut(s)	740
BseMII	CTCAG	5 cut(s)	83, 112, 243, 375, 561
BseNI	ACTGG	2 cut(s)	163, 974
BseRI	GAGGAG	2 cut(s)	45, 314
BseXI	GCAGC	2 cut(s)	186, 882
BseYI	CCCAGC	1 cut(s)	991
BsgI	GTGCAG	1 cut(s)	810
BshFI	GGCC	2 cut(s)	886, 1058
BshTI	ACCGGT	1 cut(s)	335
BsiHKAI	GWGCWC	1 cut(s)	9
BsiSI	CCGG	3 cut(s)	266, 336, 905
BslFI	GGGAC	1 cut(s)	100
BsmAI	GTCTC	4 cut(s)	49, 67, 384, 515
BsmBI	CGTCTC	1 cut(s)	67
BsmFI	GGGAC	1 cut(s)	100
BsmI	GAATGC	1 cut(s)	321
BsnI	GGCC	2 cut(s)	886, 1058
Bso31I	GGTCTC	1 cut(s)	384
Bsp1286I	GDGCHC	2 cut(s)	9, 725
Bsp143I	GATC	4 cut(s)	118, 242, 399, 684
BspACI	CCGC	3 cut(s)	601, 666, 1055
BspANI	GGCC	2 cut(s)	886, 1058
BspCNI	CTCAG	5 cut(s)	84, 111, 244, 376, 562
BspHI	TCATGA	1 cut(s)	288
BspLI	GGNNCC	2 cut(s)	16, 35
BspOI	GCTAGC	1 cut(s)	629
BspPI	GGATC	1 cut(s)	679
BspTNI	GGTCTC	1 cut(s)	384
BsrDI	GCAATG	1 cut(s)	740
BsrFI	RCCGGY	1 cut(s)	335
BsrI	ACTGG	2 cut(s)	163, 974
BssAI	RCCGGY	1 cut(s)	335
BssECI	CCNNGG	3 cut(s)	652, 928, 984
BssMI	GATC	4 cut(s)	118, 242, 399, 684
BssT1I	CCWWGG	3 cut(s)	652, 928, 984
Bst2UI	CCWGG	2 cut(s)	282, 745
Bst4CI	ACNGT	6 cut(s)	43, 148, 237, 309, 478, 717
BstAPI	GCANNNNNTGC	2 cut(s)	199, 967
BstBAI	YACGTR	1 cut(s)	224
BstC8I	GCNNGC	4 cut(s)	204, 601, 627, 884
BstDEI	CTNAG	7 cut(s)	78, 92, 98, 252, 384, 565, 570
BstEII	GGTNACC	1 cut(s)	421
BstF5I	GGATG	3 cut(s)	622, 763, 961
BstHHI	GCGC	1 cut(s)	491
BstKTI	GATC	4 cut(s)	121, 245, 402, 687
BstMAI	GTCTC	4 cut(s)	49, 67, 384, 515
BstMBI	GATC	4 cut(s)	118, 242, 399, 684
BstMWI	GCNNNNNNNGC	5 cut(s)	199, 302, 892, 920, 967
BstNI	CCWGG	2 cut(s)	282, 745
BstNSI	RCATGY	1 cut(s)	206
BstPI	GGTNACC	1 cut(s)	421
BstSCI	CCNGG	2 cut(s)	280, 743
BstSFI	CTRYAG	1 cut(s)	474
BstV1I	GCAGC	2 cut(s)	186, 882
BstXI	CCANNNNNNTGG	1 cut(s)	991
BsuRI	GGCC	2 cut(s)	886, 1058
BtrI	CACGTC	1 cut(s)	61
BtsCI	GGATG	3 cut(s)	622, 763, 961
BtsI	GCAGTG	1 cut(s)	18
BtsIMutI	CAGTG	4 cut(s)	18, 39, 106, 314
Cac8I	GCNNGC	4 cut(s)	204, 601, 627, 884
CciI	TCATGA	1 cut(s)	288
CfoI	GCGC	1 cut(s)	491
Cfr10I	RCCGGY	1 cut(s)	335
Cfr13I	GGNCC	4 cut(s)	33, 237, 713, 885
Csp6I	GTAC	3 cut(s)	221, 513, 902
CspAI	ACCGGT	1 cut(s)	335
CviAII	CATG	4 cut(s)	30, 203, 289, 891
CviQI	GTAC	3 cut(s)	221, 513, 902
DdeI	CTNAG	7 cut(s)	78, 92, 98, 252, 384, 565, 570
DpnI	GATC	4 cut(s)	120, 244, 401, 686
DpnII	GATC	4 cut(s)	118, 242, 399, 684
DriI	GACNNNNNGTC	1 cut(s)	476
Eam1105I	GACNNNNNGTC	1 cut(s)	476
EciI	GGCGGA	1 cut(s)	681
Ecl136II	GAGCTC	1 cut(s)	7
Eco130I	CCWWGG	3 cut(s)	652, 928, 984
Eco24I	GRGCYC	2 cut(s)	9, 725
Eco31I	GGTCTC	1 cut(s)	384
Eco47I	GGWCC	3 cut(s)	33, 237, 713
Eco53kI	GAGCTC	1 cut(s)	7
Eco91I	GGTNACC	1 cut(s)	421
EcoICRI	GAGCTC	1 cut(s)	7
EcoO109I	RGGNCCY	1 cut(s)	885
EcoO65I	GGTNACC	1 cut(s)	421
EcoRII	CCWGG	2 cut(s)	280, 743
EcoT14I	CCWWGG	3 cut(s)	652, 928, 984
EcoT22I	ATGCAT	1 cut(s)	1030
EcoT38I	GRGCYC	2 cut(s)	9, 725
ErhI	CCWWGG	3 cut(s)	652, 928, 984
Esp3I	CGTCTC	1 cut(s)	67
FaeI	CATG	4 cut(s)	33, 206, 292, 894
FalI	AAGNNNNNCTT	2 cut(s)	912, 944
FaqI	GGGAC	1 cut(s)	100
FatI	CATG	4 cut(s)	29, 202, 288, 890
FauI	CCCGC	1 cut(s)	608
FbaI	TGATCA	1 cut(s)	118
Fnu4HI	GCNGC	3 cut(s)	200, 896, 1056
FokI	GGATG	3 cut(s)	629, 770, 968
FriOI	GRGCYC	2 cut(s)	9, 725
Fsp4HI	GCNGC	3 cut(s)	200, 896, 1056
FspBI	CTAG	3 cut(s)	408, 516, 626
GlaI	GCGC	1 cut(s)	490
GluI	GCNGC	3 cut(s)	200, 896, 1056
GsaI	CCCAGC	1 cut(s)	995
GsuI	CTGGAG	2 cut(s)	180, 729
HaeIII	GGCC	2 cut(s)	886, 1058
HapII	CCGG	3 cut(s)	266, 336, 905
HhaI	GCGC	1 cut(s)	491
Hin1II	CATG	4 cut(s)	33, 206, 292, 894
Hin6I	GCGC	1 cut(s)	489
HinP1I	GCGC	1 cut(s)	489
HinfI	GANTC	3 cut(s)	88, 285, 748
HpaII	CCGG	3 cut(s)	266, 336, 905
HphI	GGTGA	1 cut(s)	415
Hpy166II	GTNNAC	3 cut(s)	221, 769, 860
Hpy188I	TCNGA	3 cut(s)	93, 118, 141
Hpy188III	TCNNGA	9 cut(s)	178, 229, 240, 289, 383, 403, 726, 755, 953
Hpy8I	GTNNAC	3 cut(s)	221, 769, 860
HpyAV	CCTTC	3 cut(s)	584, 800, 929
HpyCH4III	ACNGT	6 cut(s)	43, 148, 237, 309, 478, 717
HpyCH4IV	ACGT	2 cut(s)	60, 223
HpyCH4V	TGCA	8 cut(s)	193, 202, 206, 296, 464, 827, 848, 1028
HpyF10VI	GCNNNNNNNGC	5 cut(s)	199, 302, 892, 920, 967
HpyF3I	CTNAG	7 cut(s)	78, 92, 98, 252, 384, 565, 570
HpySE526I	ACGT	2 cut(s)	60, 223
Hsp92II	CATG	4 cut(s)	33, 206, 292, 894
HspAI	GCGC	1 cut(s)	489
Ksp22I	TGATCA	1 cut(s)	118
Kzo9I	GATC	4 cut(s)	118, 242, 399, 684
LmnI	GCTCC	5 cut(s)	4, 14, 161, 668, 988
Lsp1109I	GCAGC	2 cut(s)	186, 882
LweI	GCATC	2 cut(s)	451, 1015
MaeI	CTAG	3 cut(s)	408, 516, 626
MaeII	ACGT	2 cut(s)	60, 223
MaeIII	GTNAC	2 cut(s)	421, 503
MalI	GATC	4 cut(s)	120, 244, 401, 686
MboI	GATC	4 cut(s)	118, 242, 399, 684
MboII	GAAGA	1 cut(s)	195
MhlI	GDGCHC	2 cut(s)	9, 725
MluCI	AATT	2 cut(s)	258, 346
MlyI	GAGTC	1 cut(s)	82
Mph1103I	ATGCAT	1 cut(s)	1030
MseI	TTAA	2 cut(s)	257, 443
MslI	CAYNNNNRTG	1 cut(s)	756
MspA1I	CMGCKG	1 cut(s)	497
MspI	CCGG	3 cut(s)	266, 336, 905
MspR9I	CCNGG	2 cut(s)	282, 745
Mva1269I	GAATGC	1 cut(s)	321
MvaI	CCWGG	2 cut(s)	282, 745
MwoI	GCNNNNNNNGC	5 cut(s)	199, 302, 892, 920, 967
NdeII	GATC	4 cut(s)	118, 242, 399, 684
NheI	GCTAGC	1 cut(s)	625
NlaIII	CATG	4 cut(s)	33, 206, 292, 894
NlaIV	GGNNCC	2 cut(s)	16, 35
NmuCI	GTSAC	1 cut(s)	421
NsiI	ATGCAT	1 cut(s)	1030
NspI	RCATGY	1 cut(s)	206
PaeI	GCATGC	1 cut(s)	206
PagI	TCATGA	1 cut(s)	288
PctI	GAATGC	1 cut(s)	321
PfeI	GAWTC	2 cut(s)	285, 748
PflFI	GACNNNGTC	1 cut(s)	235
PfoI	TCCNGGA	2 cut(s)	280, 743
PinAI	ACCGGT	1 cut(s)	335
PkrI	GCNGC	3 cut(s)	201, 897, 1057
PleI	GAGTC	1 cut(s)	82
PpsI	GAGTC	1 cut(s)	82
Ppu21I	YACGTR	1 cut(s)	224
PsiI	TTATAA	1 cut(s)	815
Psp124BI	GAGCTC	1 cut(s)	9
Psp6I	CCWGG	2 cut(s)	280, 743
PspEI	GGTNACC	1 cut(s)	421
PspFI	CCCAGC	1 cut(s)	991
PspGI	CCWGG	2 cut(s)	280, 743
PspN4I	GGNNCC	2 cut(s)	16, 35
PspPI	GGNCC	4 cut(s)	33, 237, 713, 885
PsyI	GACNNNGTC	1 cut(s)	235
PvuII	CAGCTG	1 cut(s)	497
RsaI	GTAC	3 cut(s)	222, 514, 903
RsaNI	GTAC	3 cut(s)	221, 513, 902
RseI	CAYNNNNRTG	1 cut(s)	756
SacI	GAGCTC	1 cut(s)	9
SaqAI	TTAA	2 cut(s)	257, 443
SatI	GCNGC	3 cut(s)	200, 896, 1056
Sau3AI	GATC	4 cut(s)	118, 242, 399, 684
Sau96I	GGNCC	4 cut(s)	33, 237, 713, 885
SchI	GAGTC	1 cut(s)	82
ScrFI	CCNGG	2 cut(s)	282, 745
SduI	GDGCHC	2 cut(s)	9, 725
SfaNI	GCATC	2 cut(s)	451, 1015
SfcI	CTRYAG	1 cut(s)	474
SinI	GGWCC	3 cut(s)	33, 237, 713
SmiMI	CAYNNNNRTG	1 cut(s)	756
SpeI	ACTAGT	1 cut(s)	515
SphI	GCATGC	1 cut(s)	206
Sse9I	AATT	2 cut(s)	258, 346
SsiI	CCGC	3 cut(s)	601, 666, 1055
SspMI	CTAG	3 cut(s)	408, 516, 626
SstI	GAGCTC	1 cut(s)	9
StyD4I	CCNGG	2 cut(s)	280, 743
StyI	CCWWGG	3 cut(s)	652, 928, 984
TaaI	ACNGT	6 cut(s)	43, 148, 237, 309, 478, 717
TaiI	ACGT	2 cut(s)	63, 226
TaqI	TCGA	2 cut(s)	469, 841
TasI	AATT	2 cut(s)	258, 346
TauI	GCSGC	1 cut(s)	1058
TfiI	GAWTC	2 cut(s)	285, 748
Tru1I	TTAA	2 cut(s)	257, 443
Tru9I	TTAA	2 cut(s)	257, 443
TscAI	CASTG	4 cut(s)	25, 46, 106, 314
TseFI	GTSAC	1 cut(s)	421
TseI	GCWGC	2 cut(s)	199, 895
Tsp45I	GTSAC	1 cut(s)	421
TspDTI	ATGAA	3 cut(s)	740, 795, 819
TspGWI	ACGGA	1 cut(s)	537
TspRI	CASTG	4 cut(s)	25, 46, 106, 314
Tth111I	GACNNNGTC	1 cut(s)	235
VpaK11BI	GGWCC	3 cut(s)	33, 237, 713
XapI	RAATTY	1 cut(s)	346
XceI	RCATGY	1 cut(s)	206
XspI	CTAG	3 cut(s)	408, 516, 626
Zsp2I	ATGCAT	1 cut(s)	1030

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.