RLG00000013964

Epimerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0003144
Physical Location & Seq
Reverse (-)
44777394 .. 44783168
5775 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013964

Sequence Viewer

Length: 957 bp
ATGGAGGTCTGCCGAGCCACTGGTTTCTCGTGGACCCATACCATCTCCACCTCCCTTCACGTTTCACTGCTCCCCTCTAAGTGGGAGACCAAGAGACTCACTGTTTGGTGTGCCTCTGACCAAACCCAGAAGGCAAATCAGATGACTGTATCAGTAACGGGAGCTACTGGTTTTATCGGTAGAAGATTGAAACCACAAAAAGCCATGGAAGAATACCTTTTTTTCCCCTCTAAAATTGTTATAAACACTAATGGCTTAAGGTTGCAGGGGTGTGATTCAAACAATCTTTTAATAATAAAGAAAGAGATCAAGCAGAGCAGGGTTAGAGTCACCTCTAAGGTCATAGATTTAATAAATGATTTACCAGATGCGGTCCGACCTTCAGTCTTGGTCAGCGCAACAGCCGTCGGTTACTATGGTACTAGTGAGACACGTGTATTTGATGAACAGAGTCCATCAGGAAATGATTACTTGGCTGAGGTTTGCAGAGAATGGGAAGGAACTGCTCTCAAAGTAAATAAGGATGTTCGGTTAGGGCTCATTCGTATTGGTGTTGTTCTTGGTAAAGATGGAGGAGCTTTAGCTAAAATGATCCCTCTCTTCATGGTTTTTGCTGGGGGACCCTTGGGCTCTGGAAAACAATGGTTTTCCTGGATTCATCTGGATGATATAGTGAACCTGATATATGAAGCTCTCTCTAATCCATCTTATAAAGGAGTTATCAATGGAACTGCACCCAACCCTGTTCGATTCACAGAAATGTGTCAACATTTGGGAAATGTCTTGGGTAGGCCTTCATGGCTGCCTGTGCCTGATTTCGCCTTGAAAGCAGTCCTTGGAGAAGGCGCTTCAGTGGTGCTGGATGGGCAAAGGGTACTACCACTTAAAGCGAAAGAGTTGGGTTTTTCTTTCAAATATTCTGATATCAAAGATGCATTAAAAGCGATTATGACATGA

Protein Analysis

319

Amino Acids

34.93

Weight (kDa)

9.2

Isoelectric Point (pI)

25.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1731 PF08338 270 - 316 1.6e-18 Domain of unknown function (DUF1731)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 242, 711
AciI CCGC 1 cut(s) 371
AclWI GGATC 1 cut(s) 586
AcuI CTGAAG 2 cut(s) 366, 834
AcvI CACGTG 1 cut(s) 434
AfaI GTAC 2 cut(s) 421, 876
AfiI CCNNNNNNNGG 1 cut(s) 81
AflII CTTAAG 1 cut(s) 256
AflIII ACRYGT 2 cut(s) 431, 433
AgsI TTSAA 4 cut(s) 190, 279, 826, 913
AhdI GACNNNNNGTC 1 cut(s) 383
AhlI ACTAGT 1 cut(s) 422
AjnI CCWGG 1 cut(s) 650
AluBI AGCT 4 cut(s) 164, 578, 584, 692
AluI AGCT 4 cut(s) 164, 578, 584, 692
Alw26I GTCTC 3 cut(s) 80, 88, 422
AlwI GGATC 1 cut(s) 586
AoxI GGCC 1 cut(s) 791
ApeKI GCWGC 1 cut(s) 802
ArsI GACNNNNNNTTYG 2 cut(s) 87, 119
AspLEI GCGC 2 cut(s) 398, 848
AspS9I GGNCC 3 cut(s) 33, 373, 620
AsuHPI GGTGA 1 cut(s) 322
AvaII GGWCC 3 cut(s) 33, 373, 620
BanII GRGCYC 2 cut(s) 540, 632
BauI CACGAG 1 cut(s) 28
BbrPI CACGTG 1 cut(s) 434
BbvCI CCTCAGC 1 cut(s) 477
BbvI GCAGC 1 cut(s) 789
BccI CCATC 5 cut(s) 50, 463, 563, 712, 857
BceAI ACGGC 1 cut(s) 389
BciT130I CCWGG 1 cut(s) 652
BcoDI GTCTC 3 cut(s) 80, 88, 422
BcuI ACTAGT 1 cut(s) 422
BfaI CTAG 1 cut(s) 423
BfoI RGCGCY 1 cut(s) 849
BfrI CTTAAG 1 cut(s) 256
BglI GCCNNNNNGGC 1 cut(s) 799
BisI GCNGC 1 cut(s) 803
BlsI GCNGC 1 cut(s) 804
Bme1390I CCNGG 1 cut(s) 652
Bme18I GGWCC 3 cut(s) 33, 373, 620
BmeRI GACNNNNNGTC 1 cut(s) 383
BmgT120I GGNCC 3 cut(s) 33, 373, 620
BmiI GGNNCC 3 cut(s) 35, 621, 622
BmrFI CCNGG 1 cut(s) 652
BmsI GCATC 2 cut(s) 358, 922
Bpu10I CCTNAGC 1 cut(s) 477
BsaAI YACGTR 1 cut(s) 434
BsaI GGTCTC 1 cut(s) 80
BsaJI CCNNGG 3 cut(s) 204, 624, 835
Bsc4I CCNNNNNNNGG 1 cut(s) 81
Bse1I ACTGG 2 cut(s) 25, 172
BseBI CCWGG 1 cut(s) 652
BseDI CCNNGG 3 cut(s) 204, 624, 835
BseGI GGATG 3 cut(s) 529, 670, 868
BseLI CCNNNNNNNGG 1 cut(s) 81
BseMII CTCAG 1 cut(s) 468
BseNI ACTGG 2 cut(s) 25, 172
BseRI GAGGAG 1 cut(s) 588
BseXI GCAGC 1 cut(s) 789
BseYI CCCAGC 1 cut(s) 614
BsgI GTGCAG 1 cut(s) 717
BshFI GGCC 1 cut(s) 793
BslFI GGGAC 1 cut(s) 633
BslI CCNNNNNNNGG 1 cut(s) 81
BsmAI GTCTC 3 cut(s) 80, 88, 422
BsmFI GGGAC 1 cut(s) 633
BsnI GGCC 1 cut(s) 793
Bso31I GGTCTC 1 cut(s) 80
Bsp1286I GDGCHC 2 cut(s) 540, 632
Bsp143I GATC 2 cut(s) 306, 591
Bsp19I CCATGG 1 cut(s) 204
BspACI CCGC 1 cut(s) 371
BspANI GGCC 1 cut(s) 793
BspCNI CTCAG 1 cut(s) 469
BspLI GGNNCC 3 cut(s) 35, 621, 622
BspPI GGATC 1 cut(s) 586
BspTI CTTAAG 1 cut(s) 256
BspTNI GGTCTC 1 cut(s) 80
BsrI ACTGG 2 cut(s) 25, 172
BssECI CCNNGG 3 cut(s) 204, 624, 835
BssMI GATC 2 cut(s) 306, 591
BssSI CACGAG 1 cut(s) 28
BssT1I CCWWGG 3 cut(s) 204, 624, 835
Bst2BI CACGAG 1 cut(s) 28
Bst2UI CCWGG 1 cut(s) 652
Bst4CI ACNGT 2 cut(s) 103, 148
Bst6I CTCTTC 1 cut(s) 605
BstAFI CTTAAG 1 cut(s) 256
BstBAI YACGTR 1 cut(s) 434
BstDEI CTNAG 3 cut(s) 78, 336, 477
BstDSI CCRYGG 1 cut(s) 204
BstF5I GGATG 3 cut(s) 529, 670, 868
BstH2I RGCGCY 1 cut(s) 849
BstHHI GCGC 2 cut(s) 398, 848
BstKTI GATC 2 cut(s) 309, 594
BstMAI GTCTC 3 cut(s) 80, 88, 422
BstMBI GATC 2 cut(s) 306, 591
BstMWI GCNNNNNNNGC 5 cut(s) 799, 808, 827, 865, 941
BstNI CCWGG 1 cut(s) 652
BstSCI CCNGG 1 cut(s) 650
BstV1I GCAGC 1 cut(s) 789
BsuRI GGCC 1 cut(s) 793
BtgI CCRYGG 1 cut(s) 204
BtsCI GGATG 3 cut(s) 529, 670, 868
BtsI GCAGTG 1 cut(s) 65
BtsIMutI CAGTG 4 cut(s) 18, 65, 99, 858
CfoI GCGC 2 cut(s) 398, 848
Cfr13I GGNCC 3 cut(s) 33, 373, 620
CpoI CGGWCCG 1 cut(s) 373
Csp6I GTAC 2 cut(s) 420, 875
CspI CGGWCCG 1 cut(s) 373
CviAII CATG 4 cut(s) 205, 604, 798, 954
CviQI GTAC 2 cut(s) 420, 875
DdeI CTNAG 3 cut(s) 78, 336, 477
DpnI GATC 2 cut(s) 308, 593
DpnII GATC 2 cut(s) 306, 591
DriI GACNNNNNGTC 1 cut(s) 383
Eam1104I CTCTTC 1 cut(s) 605
Eam1105I GACNNNNNGTC 1 cut(s) 383
EarI CTCTTC 1 cut(s) 605
Eco130I CCWWGG 3 cut(s) 204, 624, 835
Eco147I AGGCCT 1 cut(s) 793
Eco24I GRGCYC 2 cut(s) 540, 632
Eco31I GGTCTC 1 cut(s) 80
Eco32I GATATC 1 cut(s) 925
Eco47I GGWCC 3 cut(s) 33, 373, 620
Eco57I CTGAAG 2 cut(s) 366, 834
Eco72I CACGTG 1 cut(s) 434
EcoO109I RGGNCCY 1 cut(s) 620
EcoRII CCWGG 1 cut(s) 650
EcoRV GATATC 1 cut(s) 925
EcoT14I CCWWGG 3 cut(s) 204, 624, 835
EcoT22I ATGCAT 1 cut(s) 937
EcoT38I GRGCYC 2 cut(s) 540, 632
ErhI CCWWGG 3 cut(s) 204, 624, 835
FaeI CATG 4 cut(s) 208, 607, 801, 957
FalI AAGNNNNNCTT 4 cut(s) 201, 233, 819, 851
FaqI GGGAC 1 cut(s) 633
FatI CATG 4 cut(s) 204, 603, 797, 953
Fnu4HI GCNGC 1 cut(s) 803
FokI GGATG 3 cut(s) 536, 677, 875
FriOI GRGCYC 2 cut(s) 540, 632
Fsp4HI GCNGC 1 cut(s) 803
FspBI CTAG 1 cut(s) 423
GlaI GCGC 2 cut(s) 397, 847
GluI GCNGC 1 cut(s) 803
GsaI CCCAGC 1 cut(s) 618
HaeII RGCGCY 1 cut(s) 849
HaeIII GGCC 1 cut(s) 793
HhaI GCGC 2 cut(s) 398, 848
Hin1II CATG 4 cut(s) 208, 607, 801, 957
Hin6I GCGC 2 cut(s) 396, 846
HinP1I GCGC 2 cut(s) 396, 846
HincII GTYRAC 1 cut(s) 767
HindII GTYRAC 1 cut(s) 767
HinfI GANTC 6 cut(s) 96, 275, 327, 451, 655, 750
HphI GGTGA 1 cut(s) 322
Hpy166II GTNNAC 3 cut(s) 33, 676, 767
Hpy188I TCNGA 4 cut(s) 118, 141, 377, 922
Hpy188III TCNNGA 3 cut(s) 459, 633, 662
Hpy8I GTNNAC 3 cut(s) 33, 676, 767
Hpy99I CGWCG 1 cut(s) 410
HpyAV CCTTC 6 cut(s) 65, 124, 390, 491, 804, 836
HpyCH4III ACNGT 2 cut(s) 103, 148
HpyCH4IV ACGT 2 cut(s) 60, 433
HpyCH4V TGCA 4 cut(s) 265, 486, 734, 935
HpyF10VI GCNNNNNNNGC 5 cut(s) 799, 808, 827, 865, 941
HpyF3I CTNAG 3 cut(s) 78, 336, 477
HpySE526I ACGT 2 cut(s) 60, 433
Hsp92II CATG 4 cut(s) 208, 607, 801, 957
HspAI GCGC 2 cut(s) 396, 846
KflI GGGWCCC 1 cut(s) 620
Kzo9I GATC 2 cut(s) 306, 591
LmnI GCTCC 3 cut(s) 75, 161, 575
Lsp1109I GCAGC 1 cut(s) 789
LweI GCATC 2 cut(s) 358, 922
MaeI CTAG 1 cut(s) 423
MaeII ACGT 2 cut(s) 60, 433
MaeIII GTNAC 3 cut(s) 154, 328, 410
MalI GATC 2 cut(s) 308, 593
MboI GATC 2 cut(s) 306, 591
MboII GAAGA 3 cut(s) 195, 221, 592
MhlI GDGCHC 2 cut(s) 540, 632
MluCI AATT 1 cut(s) 234
MlyI GAGTC 3 cut(s) 90, 336, 460
MmeI TCCRAC 1 cut(s) 400
MnlI CCTC 8 cut(s) 61, 85, 124, 238, 343, 472, 566, 606
Mph1103I ATGCAT 1 cut(s) 937
MseI TTAA 5 cut(s) 257, 290, 350, 885, 938
MslI CAYNNNNRTG 2 cut(s) 663, 758
MspCI CTTAAG 1 cut(s) 256
MspR9I CCNGG 1 cut(s) 652
MvaI CCWGG 1 cut(s) 652
MwoI GCNNNNNNNGC 5 cut(s) 799, 808, 827, 865, 941
NcoI CCATGG 1 cut(s) 204
NdeII GATC 2 cut(s) 306, 591
NlaIII CATG 4 cut(s) 208, 607, 801, 957
NlaIV GGNNCC 3 cut(s) 35, 621, 622
NmeAIII GCCGAG 1 cut(s) 38
NmuCI GTSAC 1 cut(s) 328
NsiI ATGCAT 1 cut(s) 937
PceI AGGCCT 1 cut(s) 793
PfeI GAWTC 3 cut(s) 275, 655, 750
PfoI TCCNGGA 1 cut(s) 650
PkrI GCNGC 1 cut(s) 804
PleI GAGTC 3 cut(s) 90, 335, 459
PmaCI CACGTG 1 cut(s) 434
PmlI CACGTG 1 cut(s) 434
PpsI GAGTC 3 cut(s) 90, 335, 459
Ppu21I YACGTR 1 cut(s) 434
PpuMI RGGWCCY 1 cut(s) 620
PsiI TTATAA 2 cut(s) 242, 711
Psp5II RGGWCCY 1 cut(s) 620
Psp6I CCWGG 1 cut(s) 650
PspCI CACGTG 1 cut(s) 434
PspFI CCCAGC 1 cut(s) 614
PspGI CCWGG 1 cut(s) 650
PspN4I GGNNCC 3 cut(s) 35, 621, 622
PspPI GGNCC 3 cut(s) 33, 373, 620
PspPPI RGGWCCY 1 cut(s) 620
RsaI GTAC 2 cut(s) 421, 876
RsaNI GTAC 2 cut(s) 420, 875
RseI CAYNNNNRTG 2 cut(s) 663, 758
Rsr2I CGGWCCG 1 cut(s) 373
RsrII CGGWCCG 1 cut(s) 373
SaqAI TTAA 5 cut(s) 257, 290, 350, 885, 938
SatI GCNGC 1 cut(s) 803
Sau3AI GATC 2 cut(s) 306, 591
Sau96I GGNCC 3 cut(s) 33, 373, 620
SchI GAGTC 3 cut(s) 90, 336, 460
ScrFI CCNGG 1 cut(s) 652
SduI GDGCHC 2 cut(s) 540, 632
SfaNI GCATC 2 cut(s) 358, 922
SinI GGWCC 3 cut(s) 33, 373, 620
SmiMI CAYNNNNRTG 2 cut(s) 663, 758
SmlI CTYRAG 1 cut(s) 256
SmoI CTYRAG 1 cut(s) 256
SpeI ACTAGT 1 cut(s) 422
Sse9I AATT 1 cut(s) 234
SseBI AGGCCT 1 cut(s) 793
SsiI CCGC 1 cut(s) 371
SspI AATATT 1 cut(s) 917
SspMI CTAG 1 cut(s) 423
StuI AGGCCT 1 cut(s) 793
StyD4I CCNGG 1 cut(s) 650
StyI CCWWGG 3 cut(s) 204, 624, 835
TaaI ACNGT 2 cut(s) 103, 148
TaiI ACGT 2 cut(s) 63, 436
TaqI TCGA 1 cut(s) 748
TasI AATT 1 cut(s) 234
TfiI GAWTC 3 cut(s) 275, 655, 750
Tru1I TTAA 5 cut(s) 257, 290, 350, 885, 938
Tru9I TTAA 5 cut(s) 257, 290, 350, 885, 938
TscAI CASTG 4 cut(s) 25, 72, 106, 858
TseFI GTSAC 1 cut(s) 328
TseI GCWGC 1 cut(s) 802
Tsp45I GTSAC 1 cut(s) 328
TspDTI ATGAA 5 cut(s) 459, 592, 647, 702, 786
TspRI CASTG 4 cut(s) 25, 72, 106, 858
Vha464I CTTAAG 1 cut(s) 256
VpaK11BI GGWCC 3 cut(s) 33, 373, 620
XspI CTAG 1 cut(s) 423
Zsp2I ATGCAT 1 cut(s) 937
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.