Rh6DG146400

Epimerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0003144
Physical Location & Seq
Forward (+)
21151158 .. 21156595
5438 bp
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UTR
Exon/CDS
Intron
Rh6DG146400.1

Sequence Viewer

Length: 795 bp
ATGGAGGTCTGCAGAGCCACTGCTTTCTCATGGACCCATACCATCTCCACCTCCCTTCACGTTTCTCTGCCCCCCTCTAAGTGGGAGACTAAGAGACTCACTGTTTGGTGTGCCTCTGACCAAACCCAGAAGATAAAGAAAGAGATCAAGCAGAGCAGGGTTAGAGTCACCTCTAAGGTCATAGATTTAATAAATGATTTACCAGATGCGGTCCGACCTTCAGTCTTGGTTAGTGCAACAGCCGTCGGTTACTATGGTACTAGTGAGACACGCGTATTTGATGAACAGAGTCCATCAGGAAATGATTACTTGGCTGAGGTTTGCAGAGAATGGGAAGGAACTGCTCTCAAAGTAAATAAGGATGTTCGGTTAGCGCTCATTCGTATTGGTGTTGTTCTTGGTAAAGATGGAGGAGCTTTAGCTAAAATGATCCCTCTCTTCATGGTTTTTGCTGGGGGACCCTTGGGCTCTGGAAAACAATGGTTTTCCTGGATTCATCTGGATGATATAGTGAACCTGATATATGAAGCTCTCTCTAATCCATCTTATAAAGGAGTTATCAATGGAACTGCACCCAACCCTGTTCGATTCACAGAAATGTGTCAACATTTGGGAAATGTCTTGGGTAGGCCTTCATGGCTGCCTGTGCCTGATTTCGCCTTGAAAGCAGTCCTTGGAGAAGGTGCTTCAGTGGTGCTGGATGGGCAAAGGGTACTACCACTTAAAGCGAAAGAGTTGGGTTTTTCTTTCAAATATTCTTATATCAAAGATGCATTAAAAGCGATTATGGCATGA

Protein Analysis

264

Amino Acids

29.02

Weight (kDa)

9.08

Isoelectric Point (pI)

25.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1731 PF08338 216 - 262 1.1e-17 Domain of unknown function (DUF1731)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 549
AccII CGCG 1 cut(s) 273
AciI CCGC 1 cut(s) 209
AclWI GGATC 1 cut(s) 424
AcuI CTGAAG 2 cut(s) 204, 672
AfaI GTAC 2 cut(s) 259, 714
AfeI AGCGCT 1 cut(s) 375
AfiI CCNNNNNNNGG 1 cut(s) 81
AflIII ACRYGT 1 cut(s) 271
AgsI TTSAA 2 cut(s) 664, 751
AhdI GACNNNNNGTC 1 cut(s) 221
AhlI ACTAGT 1 cut(s) 260
AjnI CCWGG 1 cut(s) 488
AluBI AGCT 3 cut(s) 416, 422, 530
AluI AGCT 3 cut(s) 416, 422, 530
Alw26I GTCTC 3 cut(s) 80, 88, 260
AlwI GGATC 1 cut(s) 424
Aor51HI AGCGCT 1 cut(s) 375
AoxI GGCC 1 cut(s) 629
ApeKI GCWGC 1 cut(s) 640
ArsI GACNNNNNNTTYG 2 cut(s) 87, 119
AspLEI GCGC 1 cut(s) 376
AspS9I GGNCC 3 cut(s) 33, 211, 458
AsuHPI GGTGA 1 cut(s) 160
AvaII GGWCC 3 cut(s) 33, 211, 458
BanII GRGCYC 1 cut(s) 470
BbvCI CCTCAGC 1 cut(s) 315
BbvI GCAGC 1 cut(s) 627
BccI CCATC 5 cut(s) 50, 301, 401, 550, 695
BceAI ACGGC 1 cut(s) 227
BciT130I CCWGG 1 cut(s) 490
BcoDI GTCTC 3 cut(s) 80, 88, 260
BcuI ACTAGT 1 cut(s) 260
BfaI CTAG 1 cut(s) 261
BfmI CTRYAG 1 cut(s) 10
BfoI RGCGCY 1 cut(s) 377
BglI GCCNNNNNGGC 1 cut(s) 637
BisI GCNGC 1 cut(s) 641
BlsI GCNGC 1 cut(s) 642
Bme1390I CCNGG 1 cut(s) 490
Bme18I GGWCC 3 cut(s) 33, 211, 458
BmeRI GACNNNNNGTC 1 cut(s) 221
BmgT120I GGNCC 3 cut(s) 33, 211, 458
BmiI GGNNCC 3 cut(s) 35, 459, 460
BmrFI CCNGG 1 cut(s) 490
BmsI GCATC 2 cut(s) 196, 760
Bpu10I CCTNAGC 1 cut(s) 315
BsaJI CCNNGG 2 cut(s) 462, 673
Bsc4I CCNNNNNNNGG 1 cut(s) 81
BseBI CCWGG 1 cut(s) 490
BseDI CCNNGG 2 cut(s) 462, 673
BseGI GGATG 3 cut(s) 367, 508, 706
BseLI CCNNNNNNNGG 1 cut(s) 81
BseMII CTCAG 1 cut(s) 306
BseRI GAGGAG 1 cut(s) 426
BseXI GCAGC 1 cut(s) 627
BseYI CCCAGC 1 cut(s) 452
BsgI GTGCAG 1 cut(s) 555
Bsh1236I CGCG 1 cut(s) 273
BshFI GGCC 1 cut(s) 631
BslFI GGGAC 1 cut(s) 471
BslI CCNNNNNNNGG 1 cut(s) 81
BsmAI GTCTC 3 cut(s) 80, 88, 260
BsmFI GGGAC 1 cut(s) 471
BsnI GGCC 1 cut(s) 631
Bsp1286I GDGCHC 1 cut(s) 470
Bsp143I GATC 2 cut(s) 144, 429
BspACI CCGC 1 cut(s) 209
BspANI GGCC 1 cut(s) 631
BspCNI CTCAG 1 cut(s) 307
BspFNI CGCG 1 cut(s) 273
BspLI GGNNCC 3 cut(s) 35, 459, 460
BspMAI CTGCAG 1 cut(s) 14
BspPI GGATC 1 cut(s) 424
BssECI CCNNGG 2 cut(s) 462, 673
BssMI GATC 2 cut(s) 144, 429
BssT1I CCWWGG 2 cut(s) 462, 673
Bst2UI CCWGG 1 cut(s) 490
Bst4CI ACNGT 1 cut(s) 103
Bst6I CTCTTC 1 cut(s) 443
BstDEI CTNAG 4 cut(s) 78, 90, 174, 315
BstF5I GGATG 3 cut(s) 367, 508, 706
BstFNI CGCG 1 cut(s) 273
BstH2I RGCGCY 1 cut(s) 377
BstHHI GCGC 1 cut(s) 376
BstKTI GATC 2 cut(s) 147, 432
BstMAI GTCTC 3 cut(s) 80, 88, 260
BstMBI GATC 2 cut(s) 144, 429
BstMWI GCNNNNNNNGC 6 cut(s) 637, 646, 665, 703, 779, 788
BstNI CCWGG 1 cut(s) 490
BstSCI CCNGG 1 cut(s) 488
BstSFI CTRYAG 1 cut(s) 10
BstUI CGCG 1 cut(s) 273
BstV1I GCAGC 1 cut(s) 627
BsuRI GGCC 1 cut(s) 631
BtsCI GGATG 3 cut(s) 367, 508, 706
BtsI GCAGTG 1 cut(s) 18
BtsIMutI CAGTG 3 cut(s) 18, 99, 696
CfoI GCGC 1 cut(s) 376
Cfr13I GGNCC 3 cut(s) 33, 211, 458
CpoI CGGWCCG 1 cut(s) 211
Csp6I GTAC 2 cut(s) 258, 713
CspI CGGWCCG 1 cut(s) 211
CviAII CATG 4 cut(s) 30, 442, 636, 792
CviJI RGCY 9 cut(s) 17, 242, 314, 416, 422, 468, 530, 631, 640
CviKI_1 RGCY 9 cut(s) 17, 242, 314, 416, 422, 468, 530, 631, 640
CviQI GTAC 2 cut(s) 258, 713
DdeI CTNAG 4 cut(s) 78, 90, 174, 315
DpnI GATC 2 cut(s) 146, 431
DpnII GATC 2 cut(s) 144, 429
DriI GACNNNNNGTC 1 cut(s) 221
Eam1104I CTCTTC 1 cut(s) 443
Eam1105I GACNNNNNGTC 1 cut(s) 221
EarI CTCTTC 1 cut(s) 443
Eco130I CCWWGG 2 cut(s) 462, 673
Eco147I AGGCCT 1 cut(s) 631
Eco24I GRGCYC 1 cut(s) 470
Eco47I GGWCC 3 cut(s) 33, 211, 458
Eco47III AGCGCT 1 cut(s) 375
Eco57I CTGAAG 2 cut(s) 204, 672
EcoO109I RGGNCCY 1 cut(s) 458
EcoRII CCWGG 1 cut(s) 488
EcoT14I CCWWGG 2 cut(s) 462, 673
EcoT22I ATGCAT 1 cut(s) 775
EcoT38I GRGCYC 1 cut(s) 470
ErhI CCWWGG 2 cut(s) 462, 673
FaeI CATG 4 cut(s) 33, 445, 639, 795
FalI AAGNNNNNCTT 2 cut(s) 657, 689
FaqI GGGAC 1 cut(s) 471
FatI CATG 4 cut(s) 29, 441, 635, 791
Fnu4HI GCNGC 1 cut(s) 641
FokI GGATG 3 cut(s) 374, 515, 713
FriOI GRGCYC 1 cut(s) 470
Fsp4HI GCNGC 1 cut(s) 641
FspBI CTAG 1 cut(s) 261
GlaI GCGC 1 cut(s) 375
GluI GCNGC 1 cut(s) 641
GsaI CCCAGC 1 cut(s) 456
HaeII RGCGCY 1 cut(s) 377
HaeIII GGCC 1 cut(s) 631
HhaI GCGC 1 cut(s) 376
Hin1II CATG 4 cut(s) 33, 445, 639, 795
Hin6I GCGC 1 cut(s) 374
HinP1I GCGC 1 cut(s) 374
HincII GTYRAC 1 cut(s) 605
HindII GTYRAC 1 cut(s) 605
HinfI GANTC 5 cut(s) 96, 165, 289, 493, 588
HphI GGTGA 1 cut(s) 160
Hpy166II GTNNAC 2 cut(s) 514, 605
Hpy188I TCNGA 2 cut(s) 118, 215
Hpy188III TCNNGA 3 cut(s) 297, 471, 500
Hpy8I GTNNAC 2 cut(s) 514, 605
Hpy99I CGWCG 1 cut(s) 248
HpyAV CCTTC 5 cut(s) 65, 228, 329, 642, 674
HpyCH4III ACNGT 1 cut(s) 103
HpyCH4IV ACGT 1 cut(s) 60
HpyCH4V TGCA 5 cut(s) 12, 236, 324, 572, 773
HpyF10VI GCNNNNNNNGC 6 cut(s) 637, 646, 665, 703, 779, 788
HpyF3I CTNAG 4 cut(s) 78, 90, 174, 315
HpySE526I ACGT 1 cut(s) 60
Hsp92II CATG 4 cut(s) 33, 445, 639, 795
HspAI GCGC 1 cut(s) 374
KflI GGGWCCC 1 cut(s) 458
Kzo9I GATC 2 cut(s) 144, 429
LmnI GCTCC 1 cut(s) 413
Lsp1109I GCAGC 1 cut(s) 627
LweI GCATC 2 cut(s) 196, 760
MaeI CTAG 1 cut(s) 261
MaeII ACGT 1 cut(s) 60
MaeIII GTNAC 2 cut(s) 166, 248
MalI GATC 2 cut(s) 146, 431
MboI GATC 2 cut(s) 144, 429
MboII GAAGA 2 cut(s) 142, 430
MhlI GDGCHC 1 cut(s) 470
MluI ACGCGT 1 cut(s) 271
MlyI GAGTC 3 cut(s) 90, 174, 298
MmeI TCCRAC 1 cut(s) 238
MnlI CCTC 7 cut(s) 61, 85, 124, 181, 310, 404, 444
Mph1103I ATGCAT 1 cut(s) 775
MseI TTAA 3 cut(s) 188, 723, 776
MslI CAYNNNNRTG 2 cut(s) 501, 596
MspR9I CCNGG 1 cut(s) 490
MvaI CCWGG 1 cut(s) 490
MvnI CGCG 1 cut(s) 273
MwoI GCNNNNNNNGC 6 cut(s) 637, 646, 665, 703, 779, 788
NdeII GATC 2 cut(s) 144, 429
NlaIII CATG 4 cut(s) 33, 445, 639, 795
NlaIV GGNNCC 3 cut(s) 35, 459, 460
NmuCI GTSAC 1 cut(s) 166
NsiI ATGCAT 1 cut(s) 775
PceI AGGCCT 1 cut(s) 631
PfeI GAWTC 2 cut(s) 493, 588
PfoI TCCNGGA 1 cut(s) 488
PkrI GCNGC 1 cut(s) 642
PleI GAGTC 3 cut(s) 90, 173, 297
PpsI GAGTC 3 cut(s) 90, 173, 297
PpuMI RGGWCCY 1 cut(s) 458
PsiI TTATAA 1 cut(s) 549
Psp5II RGGWCCY 1 cut(s) 458
Psp6I CCWGG 1 cut(s) 488
PspFI CCCAGC 1 cut(s) 452
PspGI CCWGG 1 cut(s) 488
PspN4I GGNNCC 3 cut(s) 35, 459, 460
PspPI GGNCC 3 cut(s) 33, 211, 458
PspPPI RGGWCCY 1 cut(s) 458
PstI CTGCAG 1 cut(s) 14
RsaI GTAC 2 cut(s) 259, 714
RsaNI GTAC 2 cut(s) 258, 713
RseI CAYNNNNRTG 2 cut(s) 501, 596
Rsr2I CGGWCCG 1 cut(s) 211
RsrII CGGWCCG 1 cut(s) 211
SaqAI TTAA 3 cut(s) 188, 723, 776
SatI GCNGC 1 cut(s) 641
Sau3AI GATC 2 cut(s) 144, 429
Sau96I GGNCC 3 cut(s) 33, 211, 458
SchI GAGTC 3 cut(s) 90, 174, 298
ScrFI CCNGG 1 cut(s) 490
SduI GDGCHC 1 cut(s) 470
SfaNI GCATC 2 cut(s) 196, 760
SfcI CTRYAG 1 cut(s) 10
SinI GGWCC 3 cut(s) 33, 211, 458
SmiMI CAYNNNNRTG 2 cut(s) 501, 596
SpeI ACTAGT 1 cut(s) 260
SseBI AGGCCT 1 cut(s) 631
SsiI CCGC 1 cut(s) 209
SspI AATATT 1 cut(s) 755
SspMI CTAG 1 cut(s) 261
StuI AGGCCT 1 cut(s) 631
StyD4I CCNGG 1 cut(s) 488
StyI CCWWGG 2 cut(s) 462, 673
TaaI ACNGT 1 cut(s) 103
TaiI ACGT 1 cut(s) 63
TaqI TCGA 1 cut(s) 586
TfiI GAWTC 2 cut(s) 493, 588
Tru1I TTAA 3 cut(s) 188, 723, 776
Tru9I TTAA 3 cut(s) 188, 723, 776
TscAI CASTG 3 cut(s) 25, 106, 696
TseFI GTSAC 1 cut(s) 166
TseI GCWGC 1 cut(s) 640
Tsp45I GTSAC 1 cut(s) 166
TspDTI ATGAA 5 cut(s) 297, 430, 485, 540, 624
TspRI CASTG 3 cut(s) 25, 106, 696
VpaK11BI GGWCC 3 cut(s) 33, 211, 458
XspI CTAG 1 cut(s) 261
Zsp2I ATGCAT 1 cut(s) 775
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.