Rh6DG145700

Epimerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0003144
Physical Location & Seq
Forward (+)
21042957 .. 21048394
5438 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG145700.1

Sequence Viewer

Length: 669 bp
ATGGAGATAAAGAAAGAGATCAAGCAGAGCAGGGTTAGAGTCACCTCTAAGGTAAGCAATTTAATAAATGATTTACCAGATGCGGTCCGACCTTCAGTCTTGGTTAGTGCAACAGCCGTCGGTTACTATGGTACTAGTGAGACACGCGTATTTGATGAACAGAGTCCATCAGGAAATGATTACTTGGCTGAGGTTTGCAGAGAATGGGAAGGAACTGCTCTCAAAGTAAATAAGGATGTTCGGTTAGCGCTCATTCGTATTGGTGTTGTTCTTGGTAAAGATGGAGGAGCTTTAGCTAAAATGATCCCTCTCTTCATGGTTTTTGCTGGGGGACCCTTGGGCTCTGGAAAACAATGGTTTTCCTGGATTCATCTGGATGATATAGTGAACCTGATATATGAAGCTCTCTCTAATCCATCTTATAAAGGAGTTATCAATGGAACTGCACCCAACCCTGTTCGATTCACAGAAATGTGTCAACATTTGGGAAATGTCTTGGGTAGGCCTTCATGGCTGCCTGTGCCTGATTTCGCCTTGAAAGCAGTCCTTGGAGAAGGTGCTTCAGTGGTGCTGGATGGGCAAAGGGTACTACCACTTAAAGCGAAAGAGTTGGGTTTTTCTTTCAAATATTCTTATATCAAAGATGCATTAAAAGCGATTATGGCATGA

Protein Analysis

222

Amino Acids

24.23

Weight (kDa)

8.98

Isoelectric Point (pI)

27.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1731 PF08338 174 - 220 8.1e-18 Domain of unknown function (DUF1731)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 423
AccII CGCG 1 cut(s) 147
AciI CCGC 1 cut(s) 83
AclWI GGATC 1 cut(s) 298
AcuI CTGAAG 2 cut(s) 78, 546
AfaI GTAC 2 cut(s) 133, 588
AfeI AGCGCT 1 cut(s) 249
AflIII ACRYGT 1 cut(s) 145
AgsI TTSAA 2 cut(s) 538, 625
AhdI GACNNNNNGTC 1 cut(s) 95
AhlI ACTAGT 1 cut(s) 134
AjnI CCWGG 1 cut(s) 362
AluBI AGCT 3 cut(s) 290, 296, 404
AluI AGCT 3 cut(s) 290, 296, 404
Alw26I GTCTC 1 cut(s) 134
AlwI GGATC 1 cut(s) 298
Aor51HI AGCGCT 1 cut(s) 249
AoxI GGCC 1 cut(s) 503
ApeKI GCWGC 1 cut(s) 514
AspLEI GCGC 1 cut(s) 250
AspS9I GGNCC 2 cut(s) 85, 332
AsuHPI GGTGA 1 cut(s) 34
AvaII GGWCC 2 cut(s) 85, 332
BanII GRGCYC 1 cut(s) 344
BbvCI CCTCAGC 1 cut(s) 189
BbvI GCAGC 1 cut(s) 501
BccI CCATC 4 cut(s) 175, 275, 424, 569
BceAI ACGGC 1 cut(s) 101
BciT130I CCWGG 1 cut(s) 364
BcoDI GTCTC 1 cut(s) 134
BcuI ACTAGT 1 cut(s) 134
BfaI CTAG 1 cut(s) 135
BfoI RGCGCY 1 cut(s) 251
BglI GCCNNNNNGGC 1 cut(s) 511
BisI GCNGC 1 cut(s) 515
BlsI GCNGC 1 cut(s) 516
Bme1390I CCNGG 1 cut(s) 364
Bme18I GGWCC 2 cut(s) 85, 332
BmeRI GACNNNNNGTC 1 cut(s) 95
BmgT120I GGNCC 2 cut(s) 85, 332
BmiI GGNNCC 2 cut(s) 333, 334
BmrFI CCNGG 1 cut(s) 364
BmsI GCATC 2 cut(s) 70, 634
Bpu10I CCTNAGC 1 cut(s) 189
BsaJI CCNNGG 2 cut(s) 336, 547
BseBI CCWGG 1 cut(s) 364
BseDI CCNNGG 2 cut(s) 336, 547
BseGI GGATG 3 cut(s) 241, 382, 580
BseMII CTCAG 1 cut(s) 180
BseRI GAGGAG 1 cut(s) 300
BseXI GCAGC 1 cut(s) 501
BseYI CCCAGC 1 cut(s) 326
BsgI GTGCAG 1 cut(s) 429
Bsh1236I CGCG 1 cut(s) 147
BshFI GGCC 1 cut(s) 505
BslFI GGGAC 1 cut(s) 345
BsmAI GTCTC 1 cut(s) 134
BsmFI GGGAC 1 cut(s) 345
BsnI GGCC 1 cut(s) 505
Bsp1286I GDGCHC 1 cut(s) 344
Bsp143I GATC 2 cut(s) 18, 303
BspACI CCGC 1 cut(s) 83
BspANI GGCC 1 cut(s) 505
BspCNI CTCAG 1 cut(s) 181
BspFNI CGCG 1 cut(s) 147
BspLI GGNNCC 2 cut(s) 333, 334
BspPI GGATC 1 cut(s) 298
BssECI CCNNGG 2 cut(s) 336, 547
BssMI GATC 2 cut(s) 18, 303
BssT1I CCWWGG 2 cut(s) 336, 547
Bst2UI CCWGG 1 cut(s) 364
Bst6I CTCTTC 1 cut(s) 317
BstDEI CTNAG 2 cut(s) 48, 189
BstF5I GGATG 3 cut(s) 241, 382, 580
BstFNI CGCG 1 cut(s) 147
BstH2I RGCGCY 1 cut(s) 251
BstHHI GCGC 1 cut(s) 250
BstKTI GATC 2 cut(s) 21, 306
BstMAI GTCTC 1 cut(s) 134
BstMBI GATC 2 cut(s) 18, 303
BstMWI GCNNNNNNNGC 6 cut(s) 511, 520, 539, 577, 653, 662
BstNI CCWGG 1 cut(s) 364
BstSCI CCNGG 1 cut(s) 362
BstUI CGCG 1 cut(s) 147
BstV1I GCAGC 1 cut(s) 501
BsuRI GGCC 1 cut(s) 505
BtsCI GGATG 3 cut(s) 241, 382, 580
BtsIMutI CAGTG 1 cut(s) 570
CfoI GCGC 1 cut(s) 250
Cfr13I GGNCC 2 cut(s) 85, 332
CpoI CGGWCCG 1 cut(s) 85
Csp6I GTAC 2 cut(s) 132, 587
CspI CGGWCCG 1 cut(s) 85
CviAII CATG 3 cut(s) 316, 510, 666
CviJI RGCY 8 cut(s) 116, 188, 290, 296, 342, 404, 505, 514
CviKI_1 RGCY 8 cut(s) 116, 188, 290, 296, 342, 404, 505, 514
CviQI GTAC 2 cut(s) 132, 587
DdeI CTNAG 2 cut(s) 48, 189
DpnI GATC 2 cut(s) 20, 305
DpnII GATC 2 cut(s) 18, 303
DriI GACNNNNNGTC 1 cut(s) 95
Eam1104I CTCTTC 1 cut(s) 317
Eam1105I GACNNNNNGTC 1 cut(s) 95
EarI CTCTTC 1 cut(s) 317
Eco130I CCWWGG 2 cut(s) 336, 547
Eco147I AGGCCT 1 cut(s) 505
Eco24I GRGCYC 1 cut(s) 344
Eco47I GGWCC 2 cut(s) 85, 332
Eco47III AGCGCT 1 cut(s) 249
Eco57I CTGAAG 2 cut(s) 78, 546
EcoO109I RGGNCCY 1 cut(s) 332
EcoRII CCWGG 1 cut(s) 362
EcoT14I CCWWGG 2 cut(s) 336, 547
EcoT22I ATGCAT 1 cut(s) 649
EcoT38I GRGCYC 1 cut(s) 344
ErhI CCWWGG 2 cut(s) 336, 547
FaeI CATG 3 cut(s) 319, 513, 669
FalI AAGNNNNNCTT 2 cut(s) 531, 563
FaqI GGGAC 1 cut(s) 345
FatI CATG 3 cut(s) 315, 509, 665
Fnu4HI GCNGC 1 cut(s) 515
FokI GGATG 3 cut(s) 248, 389, 587
FriOI GRGCYC 1 cut(s) 344
Fsp4HI GCNGC 1 cut(s) 515
FspBI CTAG 1 cut(s) 135
GlaI GCGC 1 cut(s) 249
GluI GCNGC 1 cut(s) 515
GsaI CCCAGC 1 cut(s) 330
HaeII RGCGCY 1 cut(s) 251
HaeIII GGCC 1 cut(s) 505
HhaI GCGC 1 cut(s) 250
Hin1II CATG 3 cut(s) 319, 513, 669
Hin6I GCGC 1 cut(s) 248
HinP1I GCGC 1 cut(s) 248
HincII GTYRAC 1 cut(s) 479
HindII GTYRAC 1 cut(s) 479
HinfI GANTC 4 cut(s) 39, 163, 367, 462
HphI GGTGA 1 cut(s) 34
Hpy166II GTNNAC 2 cut(s) 388, 479
Hpy188I TCNGA 1 cut(s) 89
Hpy188III TCNNGA 3 cut(s) 171, 345, 374
Hpy8I GTNNAC 2 cut(s) 388, 479
Hpy99I CGWCG 1 cut(s) 122
HpyAV CCTTC 4 cut(s) 102, 203, 516, 548
HpyCH4V TGCA 4 cut(s) 110, 198, 446, 647
HpyF10VI GCNNNNNNNGC 6 cut(s) 511, 520, 539, 577, 653, 662
HpyF3I CTNAG 2 cut(s) 48, 189
Hsp92II CATG 3 cut(s) 319, 513, 669
HspAI GCGC 1 cut(s) 248
KflI GGGWCCC 1 cut(s) 332
Kzo9I GATC 2 cut(s) 18, 303
LmnI GCTCC 1 cut(s) 287
Lsp1109I GCAGC 1 cut(s) 501
LweI GCATC 2 cut(s) 70, 634
MaeI CTAG 1 cut(s) 135
MaeIII GTNAC 2 cut(s) 40, 122
MalI GATC 2 cut(s) 20, 305
MboI GATC 2 cut(s) 18, 303
MboII GAAGA 1 cut(s) 304
MhlI GDGCHC 1 cut(s) 344
MluCI AATT 1 cut(s) 58
MluI ACGCGT 1 cut(s) 145
MlyI GAGTC 2 cut(s) 48, 172
MmeI TCCRAC 1 cut(s) 112
MnlI CCTC 4 cut(s) 55, 184, 278, 318
Mph1103I ATGCAT 1 cut(s) 649
MseI TTAA 3 cut(s) 62, 597, 650
MslI CAYNNNNRTG 2 cut(s) 375, 470
MspR9I CCNGG 1 cut(s) 364
MvaI CCWGG 1 cut(s) 364
MvnI CGCG 1 cut(s) 147
MwoI GCNNNNNNNGC 6 cut(s) 511, 520, 539, 577, 653, 662
NdeII GATC 2 cut(s) 18, 303
NlaIII CATG 3 cut(s) 319, 513, 669
NlaIV GGNNCC 2 cut(s) 333, 334
NmuCI GTSAC 1 cut(s) 40
NsiI ATGCAT 1 cut(s) 649
PceI AGGCCT 1 cut(s) 505
PfeI GAWTC 2 cut(s) 367, 462
PfoI TCCNGGA 1 cut(s) 362
PkrI GCNGC 1 cut(s) 516
PleI GAGTC 2 cut(s) 47, 171
PpsI GAGTC 2 cut(s) 47, 171
PpuMI RGGWCCY 1 cut(s) 332
PsiI TTATAA 1 cut(s) 423
Psp5II RGGWCCY 1 cut(s) 332
Psp6I CCWGG 1 cut(s) 362
PspFI CCCAGC 1 cut(s) 326
PspGI CCWGG 1 cut(s) 362
PspN4I GGNNCC 2 cut(s) 333, 334
PspPI GGNCC 2 cut(s) 85, 332
PspPPI RGGWCCY 1 cut(s) 332
RsaI GTAC 2 cut(s) 133, 588
RsaNI GTAC 2 cut(s) 132, 587
RseI CAYNNNNRTG 2 cut(s) 375, 470
Rsr2I CGGWCCG 1 cut(s) 85
RsrII CGGWCCG 1 cut(s) 85
SaqAI TTAA 3 cut(s) 62, 597, 650
SatI GCNGC 1 cut(s) 515
Sau3AI GATC 2 cut(s) 18, 303
Sau96I GGNCC 2 cut(s) 85, 332
SchI GAGTC 2 cut(s) 48, 172
ScrFI CCNGG 1 cut(s) 364
SduI GDGCHC 1 cut(s) 344
SetI ASST 9 cut(s) 47, 54, 94, 195, 292, 298, 393, 406, 559
SfaNI GCATC 2 cut(s) 70, 634
SinI GGWCC 2 cut(s) 85, 332
SmiMI CAYNNNNRTG 2 cut(s) 375, 470
SpeI ACTAGT 1 cut(s) 134
Sse9I AATT 1 cut(s) 58
SseBI AGGCCT 1 cut(s) 505
SsiI CCGC 1 cut(s) 83
SspI AATATT 1 cut(s) 629
SspMI CTAG 1 cut(s) 135
StuI AGGCCT 1 cut(s) 505
StyD4I CCNGG 1 cut(s) 362
StyI CCWWGG 2 cut(s) 336, 547
TaqI TCGA 1 cut(s) 460
TasI AATT 1 cut(s) 58
TfiI GAWTC 2 cut(s) 367, 462
Tru1I TTAA 3 cut(s) 62, 597, 650
Tru9I TTAA 3 cut(s) 62, 597, 650
TscAI CASTG 1 cut(s) 570
TseFI GTSAC 1 cut(s) 40
TseI GCWGC 1 cut(s) 514
Tsp45I GTSAC 1 cut(s) 40
TspDTI ATGAA 5 cut(s) 171, 304, 359, 414, 498
TspRI CASTG 1 cut(s) 570
VpaK11BI GGWCC 2 cut(s) 85, 332
XspI CTAG 1 cut(s) 135
Zsp2I ATGCAT 1 cut(s) 649
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.