Rroxscaffold_7G00200240

Epimerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0003144
Physical Location & Seq
Reverse (-)
47576285 .. 47580175
3891 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00200240.1

Sequence Viewer

Length: 972 bp
ATGAATGTTTCCTTGCTATTTGTAGTCAAGGACTTTACAGGAATCGTAATTGCAGAGGAGCCAGAGTGGAAAGATAGCATTCGAGGTTCAACTGGTGTTGTAAATTTGGCTGGAATGCCCATTAGTACAAGATGGACTTCTGAGATAAAGAAAGAGATCAAGCAGAGCAGGGTTAGAGTCACCTCTAAGGTCATAGATTTAATAAATGATTTACCAGGTGCGGTCCGACCTTCAGTCTTGGTTAGTGCAACAGCCGTCGGTTACTATGGTACTAGTGAGACAAACGTATTTGATGAACAGAGTCCATCAGGAAATGATTACTTGGCTGAGTTTTCTGATAATGCTGTATGTATAATTCGCTTGGTCAACAGCACAGCCAATGTAGACAAGTTAAAGTTAGTTGTTATTATGGCTCCTGGTCCACTTTGGGATTGTCCTGCTACAAGTGATCGATTCTACCATGGAATACGGATAATTATTATCTCTAGAGAATGGGAAGGAACTGCTCTCAAAGTAAATAAGGATGTTCGGTTAGCGCTCATTCGTATTGGTGTTGTTCTTGGTAAAGATGGAGGAGCTTTAGCTAAAATGATCCCTCTCTTCATGGTTTTTGCTGGGGGACCCTTGGGCTCTGGAAAACAATGGTATGGTACTCTAGGGTTTTCCTGGATTCATCTGGATGATATAGTGAACCTGATATATGAAGCTCTCTCTAATCCATCTTATAAAGGAGTTATCAATGGAACTGCACCCAACCCTGTTCGATTCACAGAAATGTGTCAACATTTGGGAAATGTCTTGGGTAGGCCTTCATGGCTGCCTGTGCCTGATTTCGCCTTGAAAGCAGTCCTTGGAGAAGGCGCTTCAGTGGCGCTGGATGGGCAAAGGGTACTACCACTTAAAGCGAAAGAGTTGGGTTTTTCTTTCAAATATTCTTATGTCAAAGATGCATTAAAAGCGATTATGACATGA

Protein Analysis

323

Amino Acids

35.17

Weight (kDa)

8.62

Isoelectric Point (pI)

27.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1731 PF08338 275 - 321 7.9e-17 Domain of unknown function (DUF1731)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 726
AccI GTMKAC 1 cut(s) 384
AciI CCGC 1 cut(s) 221
AclWI GGATC 1 cut(s) 586
AcsI RAATTY 1 cut(s) 103
AcuI CTGAAG 2 cut(s) 216, 849
AfaI GTAC 4 cut(s) 127, 271, 652, 891
AfeI AGCGCT 1 cut(s) 537
AgsI TTSAA 3 cut(s) 90, 841, 928
AhdI GACNNNNNGTC 1 cut(s) 233
AhlI ACTAGT 1 cut(s) 272
AjnI CCWGG 3 cut(s) 214, 415, 665
AluBI AGCT 3 cut(s) 578, 584, 707
AluI AGCT 3 cut(s) 578, 584, 707
Alw26I GTCTC 1 cut(s) 272
AlwI GGATC 1 cut(s) 586
Aor51HI AGCGCT 1 cut(s) 537
AoxI GGCC 1 cut(s) 806
ApeKI GCWGC 1 cut(s) 817
ApoI RAATTY 1 cut(s) 103
AspLEI GCGC 3 cut(s) 538, 863, 874
AspS9I GGNCC 3 cut(s) 223, 419, 620
AsuHPI GGTGA 1 cut(s) 172
AvaII GGWCC 3 cut(s) 223, 419, 620
BanII GRGCYC 1 cut(s) 632
BbvI GCAGC 1 cut(s) 804
BccI CCATC 5 cut(s) 126, 313, 563, 727, 872
BceAI ACGGC 1 cut(s) 239
BciT130I CCWGG 3 cut(s) 216, 417, 667
BcoDI GTCTC 1 cut(s) 272
BcuI ACTAGT 1 cut(s) 272
BfaI CTAG 3 cut(s) 273, 486, 656
BfoI RGCGCY 3 cut(s) 539, 864, 875
BglI GCCNNNNNGGC 1 cut(s) 814
BisI GCNGC 1 cut(s) 818
BlsI GCNGC 1 cut(s) 819
Bme1390I CCNGG 3 cut(s) 216, 417, 667
Bme18I GGWCC 3 cut(s) 223, 419, 620
BmeRI GACNNNNNGTC 1 cut(s) 233
BmgT120I GGNCC 3 cut(s) 223, 419, 620
BmiI GGNNCC 4 cut(s) 60, 414, 621, 622
BmrFI CCNGG 3 cut(s) 216, 417, 667
BmsI GCATC 1 cut(s) 937
Bsa29I ATCGAT 1 cut(s) 451
BsaJI CCNNGG 3 cut(s) 460, 624, 850
BsaXI ACNNNNNCTCC 2 cut(s) 50, 80
Bse1I ACTGG 1 cut(s) 97
BseBI CCWGG 3 cut(s) 216, 417, 667
BseCI ATCGAT 1 cut(s) 451
BseDI CCNNGG 3 cut(s) 460, 624, 850
BseGI GGATG 3 cut(s) 529, 685, 883
BseMII CTCAG 2 cut(s) 132, 318
BseNI ACTGG 1 cut(s) 97
BseRI GAGGAG 2 cut(s) 71, 588
BseXI GCAGC 1 cut(s) 804
BseYI CCCAGC 1 cut(s) 614
BsgI GTGCAG 1 cut(s) 732
BshFI GGCC 1 cut(s) 808
BshVI ATCGAT 1 cut(s) 451
BslFI GGGAC 1 cut(s) 633
BsmAI GTCTC 1 cut(s) 272
BsmFI GGGAC 1 cut(s) 633
BsmI GAATGC 2 cut(s) 78, 120
BsnI GGCC 1 cut(s) 808
Bsp1286I GDGCHC 1 cut(s) 632
Bsp143I GATC 3 cut(s) 156, 448, 591
Bsp19I CCATGG 1 cut(s) 460
BspACI CCGC 1 cut(s) 221
BspANI GGCC 1 cut(s) 808
BspCNI CTCAG 2 cut(s) 133, 319
BspDI ATCGAT 1 cut(s) 451
BspLI GGNNCC 4 cut(s) 60, 414, 621, 622
BspPI GGATC 1 cut(s) 586
BsrI ACTGG 1 cut(s) 97
BssECI CCNNGG 3 cut(s) 460, 624, 850
BssMI GATC 3 cut(s) 156, 448, 591
BssT1I CCWWGG 3 cut(s) 460, 624, 850
Bst2UI CCWGG 3 cut(s) 216, 417, 667
Bst6I CTCTTC 1 cut(s) 605
BstDEI CTNAG 3 cut(s) 141, 186, 327
BstDSI CCRYGG 1 cut(s) 460
BstF5I GGATG 3 cut(s) 529, 685, 883
BstH2I RGCGCY 3 cut(s) 539, 864, 875
BstHHI GCGC 3 cut(s) 538, 863, 874
BstKTI GATC 3 cut(s) 159, 451, 594
BstMAI GTCTC 1 cut(s) 272
BstMBI GATC 3 cut(s) 156, 448, 591
BstMWI GCNNNNNNNGC 6 cut(s) 814, 823, 842, 869, 880, 956
BstNI CCWGG 3 cut(s) 216, 417, 667
BstSCI CCNGG 3 cut(s) 214, 415, 665
BstV1I GCAGC 1 cut(s) 804
Bsu15I ATCGAT 1 cut(s) 451
BsuRI GGCC 1 cut(s) 808
BsuTUI ATCGAT 1 cut(s) 451
BtgI CCRYGG 1 cut(s) 460
BtsCI GGATG 3 cut(s) 529, 685, 883
BtsIMutI CAGTG 1 cut(s) 873
CfoI GCGC 3 cut(s) 538, 863, 874
Cfr13I GGNCC 3 cut(s) 223, 419, 620
ClaI ATCGAT 1 cut(s) 451
CpoI CGGWCCG 1 cut(s) 223
CsiI ACCWGGT 1 cut(s) 214
Csp6I GTAC 4 cut(s) 126, 270, 651, 890
CspI CGGWCCG 1 cut(s) 223
CviAII CATG 4 cut(s) 461, 604, 813, 969
CviQI GTAC 4 cut(s) 126, 270, 651, 890
DdeI CTNAG 3 cut(s) 141, 186, 327
DpnI GATC 3 cut(s) 158, 450, 593
DpnII GATC 3 cut(s) 156, 448, 591
DriI GACNNNNNGTC 1 cut(s) 233
Eam1104I CTCTTC 1 cut(s) 605
Eam1105I GACNNNNNGTC 1 cut(s) 233
EarI CTCTTC 1 cut(s) 605
Eco130I CCWWGG 3 cut(s) 460, 624, 850
Eco147I AGGCCT 1 cut(s) 808
Eco24I GRGCYC 1 cut(s) 632
Eco47I GGWCC 3 cut(s) 223, 419, 620
Eco47III AGCGCT 1 cut(s) 537
Eco57I CTGAAG 2 cut(s) 216, 849
EcoO109I RGGNCCY 1 cut(s) 620
EcoRII CCWGG 3 cut(s) 214, 415, 665
EcoT14I CCWWGG 3 cut(s) 460, 624, 850
EcoT22I ATGCAT 1 cut(s) 952
EcoT38I GRGCYC 1 cut(s) 632
ErhI CCWWGG 3 cut(s) 460, 624, 850
FaeI CATG 4 cut(s) 464, 607, 816, 972
FalI AAGNNNNNCTT 4 cut(s) 121, 153, 834, 866
FaqI GGGAC 1 cut(s) 633
FatI CATG 4 cut(s) 460, 603, 812, 968
FblI GTMKAC 1 cut(s) 384
Fnu4HI GCNGC 1 cut(s) 818
FokI GGATG 3 cut(s) 536, 692, 890
FriOI GRGCYC 1 cut(s) 632
Fsp4HI GCNGC 1 cut(s) 818
FspBI CTAG 3 cut(s) 273, 486, 656
GlaI GCGC 3 cut(s) 537, 862, 873
GluI GCNGC 1 cut(s) 818
GsaI CCCAGC 1 cut(s) 618
HaeII RGCGCY 3 cut(s) 539, 864, 875
HaeIII GGCC 1 cut(s) 808
HhaI GCGC 3 cut(s) 538, 863, 874
Hin1II CATG 4 cut(s) 464, 607, 816, 972
Hin6I GCGC 3 cut(s) 536, 861, 872
HinP1I GCGC 3 cut(s) 536, 861, 872
HincII GTYRAC 2 cut(s) 367, 782
HindII GTYRAC 2 cut(s) 367, 782
HinfI GANTC 6 cut(s) 42, 177, 301, 453, 670, 765
HphI GGTGA 1 cut(s) 172
Hpy166II GTNNAC 5 cut(s) 367, 385, 422, 691, 782
Hpy188I TCNGA 3 cut(s) 142, 227, 337
Hpy188III TCNNGA 4 cut(s) 309, 486, 633, 677
Hpy8I GTNNAC 5 cut(s) 367, 385, 422, 691, 782
Hpy99I CGWCG 1 cut(s) 260
HpyAV CCTTC 4 cut(s) 240, 491, 819, 851
HpyCH4IV ACGT 1 cut(s) 285
HpyCH4V TGCA 4 cut(s) 53, 248, 749, 950
HpyF10VI GCNNNNNNNGC 6 cut(s) 814, 823, 842, 869, 880, 956
HpyF3I CTNAG 3 cut(s) 141, 186, 327
HpySE526I ACGT 1 cut(s) 285
Hsp92II CATG 4 cut(s) 464, 607, 816, 972
HspAI GCGC 3 cut(s) 536, 861, 872
KflI GGGWCCC 1 cut(s) 620
Kzo9I GATC 3 cut(s) 156, 448, 591
LmnI GCTCC 3 cut(s) 58, 418, 575
Lsp1109I GCAGC 1 cut(s) 804
LweI GCATC 1 cut(s) 937
MabI ACCWGGT 1 cut(s) 214
MaeI CTAG 3 cut(s) 273, 486, 656
MaeII ACGT 1 cut(s) 285
MaeIII GTNAC 2 cut(s) 178, 260
MalI GATC 3 cut(s) 158, 450, 593
MboI GATC 3 cut(s) 156, 448, 591
MboII GAAGA 1 cut(s) 592
MhlI GDGCHC 1 cut(s) 632
MluCI AATT 4 cut(s) 48, 103, 354, 474
MlyI GAGTC 2 cut(s) 186, 310
MmeI TCCRAC 1 cut(s) 250
MnlI CCTC 5 cut(s) 49, 77, 193, 566, 606
Mph1103I ATGCAT 1 cut(s) 952
MseI TTAA 4 cut(s) 200, 392, 900, 953
MslI CAYNNNNRTG 2 cut(s) 678, 773
MspR9I CCNGG 3 cut(s) 216, 417, 667
Mva1269I GAATGC 2 cut(s) 78, 120
MvaI CCWGG 3 cut(s) 216, 417, 667
MwoI GCNNNNNNNGC 6 cut(s) 814, 823, 842, 869, 880, 956
NcoI CCATGG 1 cut(s) 460
NdeII GATC 3 cut(s) 156, 448, 591
NlaIII CATG 4 cut(s) 464, 607, 816, 972
NlaIV GGNNCC 4 cut(s) 60, 414, 621, 622
NmuCI GTSAC 1 cut(s) 178
NsiI ATGCAT 1 cut(s) 952
PceI AGGCCT 1 cut(s) 808
PctI GAATGC 2 cut(s) 78, 120
PfeI GAWTC 4 cut(s) 42, 453, 670, 765
PfoI TCCNGGA 1 cut(s) 665
PkrI GCNGC 1 cut(s) 819
PleI GAGTC 2 cut(s) 185, 309
PpsI GAGTC 2 cut(s) 185, 309
PpuMI RGGWCCY 1 cut(s) 620
PsiI TTATAA 1 cut(s) 726
Psp5II RGGWCCY 1 cut(s) 620
Psp6I CCWGG 3 cut(s) 214, 415, 665
PspFI CCCAGC 1 cut(s) 614
PspGI CCWGG 3 cut(s) 214, 415, 665
PspN4I GGNNCC 4 cut(s) 60, 414, 621, 622
PspPI GGNCC 3 cut(s) 223, 419, 620
PspPPI RGGWCCY 1 cut(s) 620
RsaI GTAC 4 cut(s) 127, 271, 652, 891
RsaNI GTAC 4 cut(s) 126, 270, 651, 890
RseI CAYNNNNRTG 2 cut(s) 678, 773
Rsr2I CGGWCCG 1 cut(s) 223
RsrII CGGWCCG 1 cut(s) 223
SaqAI TTAA 4 cut(s) 200, 392, 900, 953
SatI GCNGC 1 cut(s) 818
Sau3AI GATC 3 cut(s) 156, 448, 591
Sau96I GGNCC 3 cut(s) 223, 419, 620
SchI GAGTC 2 cut(s) 186, 310
ScrFI CCNGG 3 cut(s) 216, 417, 667
SduI GDGCHC 1 cut(s) 632
SexAI ACCWGGT 1 cut(s) 214
SfaNI GCATC 1 cut(s) 937
SinI GGWCC 3 cut(s) 223, 419, 620
SmiMI CAYNNNNRTG 2 cut(s) 678, 773
SpeI ACTAGT 1 cut(s) 272
Sse9I AATT 4 cut(s) 48, 103, 354, 474
SseBI AGGCCT 1 cut(s) 808
SsiI CCGC 1 cut(s) 221
SspI AATATT 1 cut(s) 932
SspMI CTAG 3 cut(s) 273, 486, 656
StuI AGGCCT 1 cut(s) 808
StyD4I CCNGG 3 cut(s) 214, 415, 665
StyI CCWWGG 3 cut(s) 460, 624, 850
TaiI ACGT 1 cut(s) 288
TaqI TCGA 3 cut(s) 82, 451, 763
TasI AATT 4 cut(s) 48, 103, 354, 474
TatI WGTACW 1 cut(s) 125
TfiI GAWTC 4 cut(s) 42, 453, 670, 765
Tru1I TTAA 4 cut(s) 200, 392, 900, 953
Tru9I TTAA 4 cut(s) 200, 392, 900, 953
TscAI CASTG 1 cut(s) 873
TseFI GTSAC 1 cut(s) 178
TseI GCWGC 1 cut(s) 817
Tsp45I GTSAC 1 cut(s) 178
TspDTI ATGAA 6 cut(s) 17, 309, 592, 662, 717, 801
TspGWI ACGGA 1 cut(s) 484
TspRI CASTG 1 cut(s) 873
VpaK11BI GGWCC 3 cut(s) 223, 419, 620
XapI RAATTY 1 cut(s) 103
XbaI TCTAGA 1 cut(s) 485
XmiI GTMKAC 1 cut(s) 384
XspI CTAG 3 cut(s) 273, 486, 656
Zsp2I ATGCAT 1 cut(s) 952
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.