RLG00000013948

Epimerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0003144
Physical Location & Seq
Reverse (-)
44609919 .. 44620279
10361 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013948

Sequence Viewer

Length: 834 bp
ATGCTCTTCACCTTCGACTTCCCTATTCTTGGTCAGTTTGATCTTACCAATTTGAAGATACTTTTCTTGTTCGCTCCCTACGTGCAACAGAGAGAAAAACTTCGACATCAGAACGGACCGGTGCCATTGTGGGTTTTGAAAACAATCAAGGAGATGTATCTGCAACCGCATCTGCATAGGCCGGCAGCTTTGGTCAGGTTACATCGTATGATGCTTGAAGATTTGCTGAATGGTATTGTGGATGAGATAAAGAAAGAGATCAAGCAGAGCAGGATTAGAGTCACCTCTAAGGGTTCTGAAAAGCATTTTGGTGGTAAATATGACATTGTGACTCCTAAGACATGCTTAAGACTTCTAGTTTGCAGAGAACGGGAAGGAACTGCCCTCAAAGTGAATAAGGATGTTCGGTTAGCGCTCATTCGTATTGGTGTTGTTCTTGGTAAAGATGGAGGAGCTTTAGCTAAAATGATCCCTCTCTTCATGATGTTTGCCGGGGGACCCTTGGGCTCTGGAAAACAATGGTTTTCCTGGATTCATCTGGATGACATGGTAAACCTGATATATGAAGCTCTCTCTAATCCATCTTATAAAGGAGTTATCAATGGAACTGCACCCAACCCTGTTCGATTCACAGAAATGTGTCAACATTTGGGAAATGTCTTGGGTAGGCCTTCATGGCTGCCTGTGCCTGATTTCGCCTTGAAAGCAGTCCTTGGAGAAGGTGCTTCAGTGGTGCTGGATGGGCAAAAGGTGCTACCACTTAAAGCGAAAGAGTTAGGATTTTCTTTCAAATACGCTTATATCAAAGATGCATTAAAAGCGATTATGACGTGA

Protein Analysis

278

Amino Acids

31.18

Weight (kDa)

9.7

Isoelectric Point (pI)

28.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1731 PF08338 229 - 275 4.4e-17 Domain of unknown function (DUF1731)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 588
AccB1I GGYRCC 1 cut(s) 121
AciI CCGC 1 cut(s) 167
AclWI GGATC 1 cut(s) 463
AcuI CTGAAG 1 cut(s) 711
AfeI AGCGCT 1 cut(s) 414
AfiI CCNNNNNNNGG 1 cut(s) 29
AflII CTTAAG 1 cut(s) 346
AgeI ACCGGT 1 cut(s) 118
AgsI TTSAA 5 cut(s) 55, 139, 218, 703, 790
AjiI CACGTC 1 cut(s) 831
AjnI CCWGG 1 cut(s) 527
AjuI GAANNNNNNNTTGG 2 cut(s) 291, 323
AluBI AGCT 4 cut(s) 188, 455, 461, 569
AluI AGCT 4 cut(s) 188, 455, 461, 569
AlwI GGATC 1 cut(s) 463
Aor51HI AGCGCT 1 cut(s) 414
AoxI GGCC 2 cut(s) 179, 668
ApeKI GCWGC 2 cut(s) 185, 679
ArsI GACNNNNNNTTYG 2 cut(s) 342, 374
AsiGI ACCGGT 1 cut(s) 118
Asp700I GAANNNNTTC 1 cut(s) 99
AspLEI GCGC 1 cut(s) 415
AspS9I GGNCC 2 cut(s) 116, 497
AsuC2I CCSGG 1 cut(s) 493
AsuHPI GGTGA 1 cut(s) 274
AvaII GGWCC 2 cut(s) 116, 497
BanI GGYRCC 1 cut(s) 121
BanII GRGCYC 1 cut(s) 509
BbvI GCAGC 2 cut(s) 197, 666
BccI CCATC 3 cut(s) 440, 589, 734
BciT130I CCWGG 1 cut(s) 529
BcnI CCSGG 1 cut(s) 493
BfaI CTAG 1 cut(s) 356
BfoI RGCGCY 1 cut(s) 416
BfrI CTTAAG 1 cut(s) 346
BglI GCCNNNNNGGC 1 cut(s) 676
BisI GCNGC 2 cut(s) 186, 680
BlsI GCNGC 2 cut(s) 187, 681
Bme1390I CCNGG 2 cut(s) 493, 529
Bme18I GGWCC 2 cut(s) 116, 497
BmgBI CACGTC 1 cut(s) 831
BmgT120I GGNCC 2 cut(s) 116, 497
BmiI GGNNCC 3 cut(s) 123, 498, 499
BmrFI CCNGG 2 cut(s) 493, 529
BmsI GCATC 3 cut(s) 178, 201, 799
BpuMI CCSGG 1 cut(s) 493
BsaAI YACGTR 1 cut(s) 82
BsaJI CCNNGG 3 cut(s) 492, 501, 712
BsaWI WCCGGW 1 cut(s) 118
Bsc4I CCNNNNNNNGG 1 cut(s) 29
Bse118I RCCGGY 2 cut(s) 118, 181
BseBI CCWGG 1 cut(s) 529
BseDI CCNNGG 3 cut(s) 492, 501, 712
BseGI GGATG 4 cut(s) 247, 406, 547, 745
BseLI CCNNNNNNNGG 1 cut(s) 29
BseRI GAGGAG 1 cut(s) 465
BseXI GCAGC 2 cut(s) 197, 666
BsgI GTGCAG 1 cut(s) 594
BshFI GGCC 2 cut(s) 181, 670
BshNI GGYRCC 1 cut(s) 121
BshTI ACCGGT 1 cut(s) 118
BsiSI CCGG 3 cut(s) 119, 182, 492
BslFI GGGAC 1 cut(s) 510
BslI CCNNNNNNNGG 1 cut(s) 29
BsmFI GGGAC 1 cut(s) 510
BsnI GGCC 2 cut(s) 181, 670
Bsp1286I GDGCHC 1 cut(s) 509
Bsp143I GATC 3 cut(s) 40, 258, 468
BspACI CCGC 1 cut(s) 167
BspANI GGCC 2 cut(s) 181, 670
BspHI TCATGA 1 cut(s) 480
BspLI GGNNCC 3 cut(s) 123, 498, 499
BspPI GGATC 1 cut(s) 463
BspQI GCTCTTC 1 cut(s) 11
BspT107I GGYRCC 1 cut(s) 121
BspTI CTTAAG 1 cut(s) 346
BsrFI RCCGGY 2 cut(s) 118, 181
BssAI RCCGGY 2 cut(s) 118, 181
BssECI CCNNGG 3 cut(s) 492, 501, 712
BssMI GATC 3 cut(s) 40, 258, 468
BssT1I CCWWGG 2 cut(s) 501, 712
Bst2UI CCWGG 1 cut(s) 529
Bst6I CTCTTC 2 cut(s) 11, 482
BstAFI CTTAAG 1 cut(s) 346
BstAPI GCANNNNNTGC 1 cut(s) 751
BstBAI YACGTR 1 cut(s) 82
BstC8I GCNNGC 1 cut(s) 183
BstDEI CTNAG 2 cut(s) 288, 336
BstF5I GGATG 4 cut(s) 247, 406, 547, 745
BstH2I RGCGCY 1 cut(s) 416
BstHHI GCGC 1 cut(s) 415
BstKTI GATC 3 cut(s) 43, 261, 471
BstMBI GATC 3 cut(s) 40, 258, 468
BstMWI GCNNNNNNNGC 6 cut(s) 676, 685, 704, 742, 751, 818
BstNI CCWGG 1 cut(s) 529
BstNSI RCATGY 1 cut(s) 345
BstSCI CCNGG 2 cut(s) 491, 527
BstV1I GCAGC 2 cut(s) 197, 666
BsuRI GGCC 2 cut(s) 181, 670
BtrI CACGTC 1 cut(s) 831
BtsCI GGATG 4 cut(s) 247, 406, 547, 745
BtsIMutI CAGTG 1 cut(s) 735
Cac8I GCNNGC 1 cut(s) 183
CciI TCATGA 1 cut(s) 480
CfoI GCGC 1 cut(s) 415
Cfr10I RCCGGY 2 cut(s) 118, 181
Cfr13I GGNCC 2 cut(s) 116, 497
CpoI CGGWCCG 1 cut(s) 116
CspAI ACCGGT 1 cut(s) 118
CspI CGGWCCG 1 cut(s) 116
CviAII CATG 4 cut(s) 342, 481, 547, 675
CviJI RGCY 8 cut(s) 181, 188, 455, 461, 507, 569, 670, 679
CviKI_1 RGCY 8 cut(s) 181, 188, 455, 461, 507, 569, 670, 679
DdeI CTNAG 2 cut(s) 288, 336
DpnI GATC 3 cut(s) 42, 260, 470
DpnII GATC 3 cut(s) 40, 258, 468
Eam1104I CTCTTC 2 cut(s) 11, 482
EarI CTCTTC 2 cut(s) 11, 482
Eco130I CCWWGG 2 cut(s) 501, 712
Eco147I AGGCCT 1 cut(s) 670
Eco24I GRGCYC 1 cut(s) 509
Eco47I GGWCC 2 cut(s) 116, 497
Eco47III AGCGCT 1 cut(s) 414
Eco57I CTGAAG 1 cut(s) 711
EcoO109I RGGNCCY 1 cut(s) 497
EcoRII CCWGG 1 cut(s) 527
EcoT14I CCWWGG 2 cut(s) 501, 712
EcoT22I ATGCAT 1 cut(s) 814
EcoT38I GRGCYC 1 cut(s) 509
ErhI CCWWGG 2 cut(s) 501, 712
FaeI CATG 4 cut(s) 345, 484, 550, 678
FalI AAGNNNNNCTT 4 cut(s) 329, 361, 696, 728
FaqI GGGAC 1 cut(s) 510
FatI CATG 4 cut(s) 341, 480, 546, 674
Fnu4HI GCNGC 2 cut(s) 186, 680
FokI GGATG 4 cut(s) 254, 413, 554, 752
FriOI GRGCYC 1 cut(s) 509
Fsp4HI GCNGC 2 cut(s) 186, 680
FspBI CTAG 1 cut(s) 356
GlaI GCGC 1 cut(s) 414
GluI GCNGC 2 cut(s) 186, 680
HaeII RGCGCY 1 cut(s) 416
HaeIII GGCC 2 cut(s) 181, 670
HapII CCGG 3 cut(s) 119, 182, 492
HhaI GCGC 1 cut(s) 415
Hin1II CATG 4 cut(s) 345, 484, 550, 678
Hin6I GCGC 1 cut(s) 413
HinP1I GCGC 1 cut(s) 413
HincII GTYRAC 1 cut(s) 644
HindII GTYRAC 1 cut(s) 644
HinfI GANTC 4 cut(s) 279, 331, 532, 627
HpaII CCGG 3 cut(s) 119, 182, 492
HphI GGTGA 1 cut(s) 274
Hpy166II GTNNAC 2 cut(s) 553, 644
Hpy188I TCNGA 2 cut(s) 111, 298
Hpy188III TCNNGA 3 cut(s) 481, 510, 539
Hpy8I GTNNAC 2 cut(s) 553, 644
HpyAV CCTTC 4 cut(s) 22, 368, 681, 713
HpyCH4IV ACGT 2 cut(s) 81, 830
HpyCH4V TGCA 6 cut(s) 85, 163, 175, 363, 611, 812
HpyF10VI GCNNNNNNNGC 6 cut(s) 676, 685, 704, 742, 751, 818
HpyF3I CTNAG 2 cut(s) 288, 336
HpySE526I ACGT 2 cut(s) 81, 830
Hsp92II CATG 4 cut(s) 345, 484, 550, 678
HspAI GCGC 1 cut(s) 413
KflI GGGWCCC 1 cut(s) 497
KroI GCCGGC 1 cut(s) 181
KroNI GCCGGC 1 cut(s) 183
Kzo9I GATC 3 cut(s) 40, 258, 468
LguI GCTCTTC 1 cut(s) 11
LmnI GCTCC 2 cut(s) 79, 452
Lsp1109I GCAGC 2 cut(s) 197, 666
LweI GCATC 3 cut(s) 178, 201, 799
MaeI CTAG 1 cut(s) 356
MaeII ACGT 2 cut(s) 81, 830
MaeIII GTNAC 3 cut(s) 198, 280, 328
MalI GATC 3 cut(s) 42, 260, 470
MboI GATC 3 cut(s) 40, 258, 468
MboII GAAGA 3 cut(s) 67, 230, 469
MhlI GDGCHC 1 cut(s) 509
MluCI AATT 1 cut(s) 49
MlyI GAGTC 2 cut(s) 288, 325
MnlI CCTC 4 cut(s) 295, 395, 443, 483
Mph1103I ATGCAT 1 cut(s) 814
MroNI GCCGGC 1 cut(s) 181
MroXI GAANNNNTTC 1 cut(s) 99
MseI TTAA 3 cut(s) 347, 762, 815
MslI CAYNNNNRTG 3 cut(s) 309, 540, 635
MspCI CTTAAG 1 cut(s) 346
MspI CCGG 3 cut(s) 119, 182, 492
MspR9I CCNGG 2 cut(s) 493, 529
MvaI CCWGG 1 cut(s) 529
MwoI GCNNNNNNNGC 6 cut(s) 676, 685, 704, 742, 751, 818
NaeI GCCGGC 1 cut(s) 183
NciI CCSGG 1 cut(s) 493
NdeII GATC 3 cut(s) 40, 258, 468
NgoMIV GCCGGC 1 cut(s) 181
NlaIII CATG 4 cut(s) 345, 484, 550, 678
NlaIV GGNNCC 3 cut(s) 123, 498, 499
NmuCI GTSAC 2 cut(s) 280, 328
NsiI ATGCAT 1 cut(s) 814
NspI RCATGY 1 cut(s) 345
PagI TCATGA 1 cut(s) 480
PceI AGGCCT 1 cut(s) 670
PciSI GCTCTTC 1 cut(s) 11
PcsI WCGNNNNNNNCGW 1 cut(s) 78
PdiI GCCGGC 1 cut(s) 183
PdmI GAANNNNTTC 1 cut(s) 99
PfeI GAWTC 2 cut(s) 532, 627
PfoI TCCNGGA 1 cut(s) 527
PinAI ACCGGT 1 cut(s) 118
PkrI GCNGC 2 cut(s) 187, 681
PleI GAGTC 2 cut(s) 287, 325
PpsI GAGTC 2 cut(s) 287, 325
Ppu21I YACGTR 1 cut(s) 82
PpuMI RGGWCCY 1 cut(s) 497
PsiI TTATAA 1 cut(s) 588
Psp5II RGGWCCY 1 cut(s) 497
Psp6I CCWGG 1 cut(s) 527
PspGI CCWGG 1 cut(s) 527
PspN4I GGNNCC 3 cut(s) 123, 498, 499
PspPI GGNCC 2 cut(s) 116, 497
PspPPI RGGWCCY 1 cut(s) 497
RseI CAYNNNNRTG 3 cut(s) 309, 540, 635
Rsr2I CGGWCCG 1 cut(s) 116
RsrII CGGWCCG 1 cut(s) 116
SapI GCTCTTC 1 cut(s) 11
SaqAI TTAA 3 cut(s) 347, 762, 815
SatI GCNGC 2 cut(s) 186, 680
Sau3AI GATC 3 cut(s) 40, 258, 468
Sau96I GGNCC 2 cut(s) 116, 497
SchI GAGTC 2 cut(s) 288, 325
ScrFI CCNGG 2 cut(s) 493, 529
SduI GDGCHC 1 cut(s) 509
SfaNI GCATC 3 cut(s) 178, 201, 799
SinI GGWCC 2 cut(s) 116, 497
SmiMI CAYNNNNRTG 3 cut(s) 309, 540, 635
SmlI CTYRAG 1 cut(s) 346
SmoI CTYRAG 1 cut(s) 346
Sse9I AATT 1 cut(s) 49
SseBI AGGCCT 1 cut(s) 670
SsiI CCGC 1 cut(s) 167
SspMI CTAG 1 cut(s) 356
StuI AGGCCT 1 cut(s) 670
StyD4I CCNGG 2 cut(s) 491, 527
StyI CCWWGG 2 cut(s) 501, 712
TaiI ACGT 2 cut(s) 84, 833
TaqI TCGA 3 cut(s) 15, 103, 625
TasI AATT 1 cut(s) 49
TfiI GAWTC 2 cut(s) 532, 627
Tru1I TTAA 3 cut(s) 347, 762, 815
Tru9I TTAA 3 cut(s) 347, 762, 815
TscAI CASTG 1 cut(s) 735
TseFI GTSAC 2 cut(s) 280, 328
TseI GCWGC 2 cut(s) 185, 679
Tsp45I GTSAC 2 cut(s) 280, 328
TspDTI ATGAA 4 cut(s) 469, 524, 579, 663
TspGWI ACGGA 1 cut(s) 129
TspRI CASTG 1 cut(s) 735
Vha464I CTTAAG 1 cut(s) 346
VpaK11BI GGWCC 2 cut(s) 116, 497
XceI RCATGY 1 cut(s) 345
XmnI GAANNNNTTC 1 cut(s) 99
XspI CTAG 1 cut(s) 356
Zsp2I ATGCAT 1 cut(s) 814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.