RLG00000013989

Epimerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0003144
Physical Location & Seq
Forward (+)
45261170 .. 45266721
5552 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013989

Sequence Viewer

Length: 564 bp
ATGGGTGATGGAAAATTGGAAACGAGTACAAGTGAGACATGCATATTTGATGAACAGAGTCCATCAGGAAATGATTACTTGGCTGAGGTTTGCAGAGAATGGGAAGGAACTGCCCTCAAAGTGAATAAGGATGTTCGGTTAGCGCTCATTCGTATTGGTGTTGTTCTTGGTAAAGATGGAGGAGCTTTAGCTAAAATGATCCCTCTCTTCATGATGTTTGCTGGGGGACCCTTGGGCTCTGGAAAACAATGGTTTTCCTGGATTCATCTGGATGACATAGTAAACCTGATATATGAAGCTCTCTCTAATCCATCTTCTAAAGGAGTTATCAATGGAACTGCACCCAACCCTGTTCGATTCACAGAAATGTGTCAACATTTGGGAAATGTCTTGGGTAGGCCTTCATGGCTGCCTGTGCCTGATTTCGCCTTGAAAGCAGTCCTTGGAGAAGGCGCTTCAGTGGTGCTGGATGGGAAAAGGGTACTGCCACTTAAAGCGAAAGAGTTGGGATTTTCTTTTAAATACGCTTATATCAAAGATGCATTAAAAGCGATTATGACGTGA

Protein Analysis

188

Amino Acids

20.26

Weight (kDa)

6.73

Isoelectric Point (pI)

25.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1731 PF08338 139 - 185 1.6e-16 Domain of unknown function (DUF1731)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 193
AcuI CTGAAG 1 cut(s) 441
AfaI GTAC 2 cut(s) 28, 483
AfeI AGCGCT 1 cut(s) 144
AgsI TTSAA 1 cut(s) 433
AjiI CACGTC 1 cut(s) 561
AjnI CCWGG 1 cut(s) 257
AluBI AGCT 3 cut(s) 185, 191, 299
AluI AGCT 3 cut(s) 185, 191, 299
Alw26I GTCTC 1 cut(s) 29
AlwI GGATC 1 cut(s) 193
Aor51HI AGCGCT 1 cut(s) 144
AoxI GGCC 1 cut(s) 398
ApeKI GCWGC 1 cut(s) 409
AspLEI GCGC 2 cut(s) 145, 455
AspS9I GGNCC 1 cut(s) 227
AsuHPI GGTGA 1 cut(s) 17
AvaII GGWCC 1 cut(s) 227
BanII GRGCYC 1 cut(s) 239
BbvCI CCTCAGC 1 cut(s) 84
BbvI GCAGC 1 cut(s) 396
BccI CCATC 5 cut(s) 2, 70, 170, 319, 464
BciT130I CCWGG 1 cut(s) 259
BcoDI GTCTC 1 cut(s) 29
BfoI RGCGCY 2 cut(s) 146, 456
BglI GCCNNNNNGGC 1 cut(s) 406
BisI GCNGC 1 cut(s) 410
BlsI GCNGC 1 cut(s) 411
Bme1390I CCNGG 1 cut(s) 259
Bme18I GGWCC 1 cut(s) 227
BmgBI CACGTC 1 cut(s) 561
BmgT120I GGNCC 1 cut(s) 227
BmiI GGNNCC 2 cut(s) 228, 229
BmrFI CCNGG 1 cut(s) 259
BmsI GCATC 1 cut(s) 529
Bpu10I CCTNAGC 1 cut(s) 84
BsaJI CCNNGG 2 cut(s) 231, 442
BseBI CCWGG 1 cut(s) 259
BseDI CCNNGG 2 cut(s) 231, 442
BseGI GGATG 3 cut(s) 136, 277, 475
BseMII CTCAG 1 cut(s) 75
BseRI GAGGAG 1 cut(s) 195
BseXI GCAGC 1 cut(s) 396
BseYI CCCAGC 1 cut(s) 221
BsgI GTGCAG 1 cut(s) 324
BshFI GGCC 1 cut(s) 400
BslFI GGGAC 1 cut(s) 240
BsmAI GTCTC 1 cut(s) 29
BsmFI GGGAC 1 cut(s) 240
BsnI GGCC 1 cut(s) 400
Bsp1286I GDGCHC 1 cut(s) 239
Bsp143I GATC 1 cut(s) 198
BspANI GGCC 1 cut(s) 400
BspCNI CTCAG 1 cut(s) 76
BspHI TCATGA 1 cut(s) 210
BspLI GGNNCC 2 cut(s) 228, 229
BspPI GGATC 1 cut(s) 193
BssECI CCNNGG 2 cut(s) 231, 442
BssMI GATC 1 cut(s) 198
BssT1I CCWWGG 2 cut(s) 231, 442
Bst2UI CCWGG 1 cut(s) 259
Bst6I CTCTTC 1 cut(s) 212
BstDEI CTNAG 1 cut(s) 84
BstF5I GGATG 3 cut(s) 136, 277, 475
BstH2I RGCGCY 2 cut(s) 146, 456
BstHHI GCGC 2 cut(s) 145, 455
BstKTI GATC 1 cut(s) 201
BstMAI GTCTC 1 cut(s) 29
BstMBI GATC 1 cut(s) 198
BstMWI GCNNNNNNNGC 4 cut(s) 406, 415, 434, 548
BstNI CCWGG 1 cut(s) 259
BstNSI RCATGY 1 cut(s) 42
BstSCI CCNGG 1 cut(s) 257
BstV1I GCAGC 1 cut(s) 396
BsuRI GGCC 1 cut(s) 400
BtrI CACGTC 1 cut(s) 561
BtsCI GGATG 3 cut(s) 136, 277, 475
BtsIMutI CAGTG 1 cut(s) 465
CciI TCATGA 1 cut(s) 210
CfoI GCGC 2 cut(s) 145, 455
Cfr13I GGNCC 1 cut(s) 227
Csp6I GTAC 2 cut(s) 27, 482
CviAII CATG 3 cut(s) 39, 211, 405
CviJI RGCY 7 cut(s) 83, 185, 191, 237, 299, 400, 409
CviKI_1 RGCY 7 cut(s) 83, 185, 191, 237, 299, 400, 409
CviQI GTAC 2 cut(s) 27, 482
DdeI CTNAG 1 cut(s) 84
DpnI GATC 1 cut(s) 200
DpnII GATC 1 cut(s) 198
DraI TTTAAA 1 cut(s) 520
Eam1104I CTCTTC 1 cut(s) 212
EarI CTCTTC 1 cut(s) 212
Eco130I CCWWGG 2 cut(s) 231, 442
Eco147I AGGCCT 1 cut(s) 400
Eco24I GRGCYC 1 cut(s) 239
Eco47I GGWCC 1 cut(s) 227
Eco47III AGCGCT 1 cut(s) 144
Eco57I CTGAAG 1 cut(s) 441
EcoO109I RGGNCCY 1 cut(s) 227
EcoRII CCWGG 1 cut(s) 257
EcoT14I CCWWGG 2 cut(s) 231, 442
EcoT22I ATGCAT 2 cut(s) 44, 544
EcoT38I GRGCYC 1 cut(s) 239
ErhI CCWWGG 2 cut(s) 231, 442
FaeI CATG 3 cut(s) 42, 214, 408
FaiI YATR 9 cut(s) 40, 44, 212, 278, 292, 294, 406, 531, 557
FalI AAGNNNNNCTT 2 cut(s) 426, 458
FaqI GGGAC 1 cut(s) 240
FatI CATG 3 cut(s) 38, 210, 404
Fnu4HI GCNGC 1 cut(s) 410
FokI GGATG 3 cut(s) 143, 284, 482
FriOI GRGCYC 1 cut(s) 239
Fsp4HI GCNGC 1 cut(s) 410
GlaI GCGC 2 cut(s) 144, 454
GluI GCNGC 1 cut(s) 410
GsaI CCCAGC 1 cut(s) 225
HaeII RGCGCY 2 cut(s) 146, 456
HaeIII GGCC 1 cut(s) 400
HhaI GCGC 2 cut(s) 145, 455
Hin1II CATG 3 cut(s) 42, 214, 408
Hin6I GCGC 2 cut(s) 143, 453
HinP1I GCGC 2 cut(s) 143, 453
HincII GTYRAC 1 cut(s) 374
HindII GTYRAC 1 cut(s) 374
HinfI GANTC 3 cut(s) 58, 262, 357
HphI GGTGA 1 cut(s) 17
Hpy166II GTNNAC 2 cut(s) 283, 374
Hpy188III TCNNGA 4 cut(s) 66, 211, 240, 269
Hpy8I GTNNAC 2 cut(s) 283, 374
HpyAV CCTTC 3 cut(s) 98, 411, 443
HpyCH4IV ACGT 1 cut(s) 560
HpyCH4V TGCA 4 cut(s) 42, 93, 341, 542
HpyF10VI GCNNNNNNNGC 4 cut(s) 406, 415, 434, 548
HpyF3I CTNAG 1 cut(s) 84
HpySE526I ACGT 1 cut(s) 560
Hsp92II CATG 3 cut(s) 42, 214, 408
HspAI GCGC 2 cut(s) 143, 453
KflI GGGWCCC 1 cut(s) 227
Kzo9I GATC 1 cut(s) 198
LmnI GCTCC 1 cut(s) 182
Lsp1109I GCAGC 1 cut(s) 396
LweI GCATC 1 cut(s) 529
MaeII ACGT 1 cut(s) 560
MalI GATC 1 cut(s) 200
MboI GATC 1 cut(s) 198
MboII GAAGA 2 cut(s) 199, 306
MhlI GDGCHC 1 cut(s) 239
MluCI AATT 1 cut(s) 14
MlyI GAGTC 1 cut(s) 67
MnlI CCTC 4 cut(s) 79, 125, 173, 213
Mph1103I ATGCAT 2 cut(s) 44, 544
MseI TTAA 3 cut(s) 492, 519, 545
MslI CAYNNNNRTG 2 cut(s) 270, 365
MspR9I CCNGG 1 cut(s) 259
MvaI CCWGG 1 cut(s) 259
MwoI GCNNNNNNNGC 4 cut(s) 406, 415, 434, 548
NdeII GATC 1 cut(s) 198
NlaIII CATG 3 cut(s) 42, 214, 408
NlaIV GGNNCC 2 cut(s) 228, 229
NsiI ATGCAT 2 cut(s) 44, 544
NspI RCATGY 1 cut(s) 42
PagI TCATGA 1 cut(s) 210
PceI AGGCCT 1 cut(s) 400
PfeI GAWTC 2 cut(s) 262, 357
PfoI TCCNGGA 1 cut(s) 257
PkrI GCNGC 1 cut(s) 411
PleI GAGTC 1 cut(s) 66
PpsI GAGTC 1 cut(s) 66
PpuMI RGGWCCY 1 cut(s) 227
Psp5II RGGWCCY 1 cut(s) 227
Psp6I CCWGG 1 cut(s) 257
PspFI CCCAGC 1 cut(s) 221
PspGI CCWGG 1 cut(s) 257
PspN4I GGNNCC 2 cut(s) 228, 229
PspPI GGNCC 1 cut(s) 227
PspPPI RGGWCCY 1 cut(s) 227
RsaI GTAC 2 cut(s) 28, 483
RsaNI GTAC 2 cut(s) 27, 482
RseI CAYNNNNRTG 2 cut(s) 270, 365
SaqAI TTAA 3 cut(s) 492, 519, 545
SatI GCNGC 1 cut(s) 410
Sau3AI GATC 1 cut(s) 198
Sau96I GGNCC 1 cut(s) 227
SchI GAGTC 1 cut(s) 67
ScrFI CCNGG 1 cut(s) 259
SduI GDGCHC 1 cut(s) 239
SetI ASST 6 cut(s) 90, 187, 193, 288, 301, 563
SfaNI GCATC 1 cut(s) 529
SinI GGWCC 1 cut(s) 227
SmiMI CAYNNNNRTG 2 cut(s) 270, 365
Sse9I AATT 1 cut(s) 14
SseBI AGGCCT 1 cut(s) 400
StuI AGGCCT 1 cut(s) 400
StyD4I CCNGG 1 cut(s) 257
StyI CCWWGG 2 cut(s) 231, 442
TaiI ACGT 1 cut(s) 563
TaqI TCGA 1 cut(s) 355
TasI AATT 1 cut(s) 14
TatI WGTACW 1 cut(s) 26
TfiI GAWTC 2 cut(s) 262, 357
Tru1I TTAA 3 cut(s) 492, 519, 545
Tru9I TTAA 3 cut(s) 492, 519, 545
TscAI CASTG 1 cut(s) 465
TseI GCWGC 1 cut(s) 409
TspDTI ATGAA 5 cut(s) 66, 199, 254, 309, 393
TspRI CASTG 1 cut(s) 465
VpaK11BI GGWCC 1 cut(s) 227
XceI RCATGY 1 cut(s) 42
Zsp2I ATGCAT 2 cut(s) 44, 544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.