Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0004434

Physical Location & Seq

Genomic Coordinates Reverse (-)

8666995 .. 8670721

Gene Structure 3727 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1063800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1500 bp

ATGAAAAAAACCCTCTCCTTCCCTCATTTTCTTGTTTCCGTTTTTACCCTAGTTGCCCTCATCGAGCTCCCAAGCTTTGCATCATCAAGCCATGACTACCAAGAGGCATTGTCCAAGGCCATACTATTCTTCGAGGGGCAGAGGTCGGGGGTCTTGCCTCAGGACCAGCGCCAATCATGGCGTGCCAATTCAGGACTCAGTGACGGGTGGACGTACAACACAGACTTGACCGGCGGCTATTACGACGCCGGTGATAATGTCAAGTTTGGTTTTCCGATGGCGTTCACGACGACAATGCTGGCTTGGAGTGTGATAGAGTTTGGAGATTCAATGCCTCCCAATGAGCTGAGGAACGCTCTCGTGGCAATCCGCTGGGCCACGGATTACTTGCTCAAGACTGTGTCTCAGCCTAACCGGATTTTTGTGCAGGTTGGTGACCCAATTATAGACCATAACTGTTGGGAAAGACCTGAAGACATGGACACAACTAGGACTGTATATGCTGTGGATTCGCCCAATCCCGCTTCGGAAGTCGCAGGTGAGACAGCAGCGGCCCTAGCAGCTTCGTCGATGGCATTCCGATCATCCGATCCTGGGTACTCGGACACATTGTTAAGAAATGCCAAGGAGGCCTTTGAGTTTGCTGATACTCACAGAGGTGCTTATAGTGATAATTCTAACATTAAAGATGGTGTCTGCCCATTTTATTGTGATTTTGATGGTTATCAAGACGAGTTGCTGTGGGGGGCAGCATGGTTGAGTAGGGTCACTCAAGATGGTGGTTACCTAGACTACATACAAAACAATGGCAAAACTCTTGGTGCCGATGACAACATAAACGAATTTGGTTGGGACAACAAGCATGCTGGCCTAAATGTTCTAGTCTCCAAGCAAGTCTTAGAAGGCAACATGTACAATCTCGAATCCTACAAATCATCGGCAGATAGCTTCATGTGCACGTTAATTCCGGACTCATCATCATCCCACATCGAGTACACTCCCGGCGGACTCATCTATAAGCCGGGAGGCAGCAATTTGCAGCATGTAACTTCCATCACATTCCTATTACTTGTTTACGCGAATTACCTTTCACGGACCTCGCAGTCCCTAAACTGCGGCAACATAAACGTCAGTCCAGCGACCCTACGCCAAATCGCCAAGAAACAAGTTGATTACATTTTGGGTGATAATCCAATGGGATTGTCATACATGGTTGGCTATGGTGACCATTTTCCGCAACGCATTCACCACCGTGGCTCTTCGTTGCCATCTATCAAGGACCACCCCCAAGCCATTGCTTGCAAGGAAGGCTCAGTGTACTTCAACTCTTCAGGCCCTAACCCTAATGTTCTTGTGGGTGCCCTCGTGGGAGGTCCAGGAGAAGACGACTTTTATGGGGATGATCGTGCAGACTTTAAGAAATCTGAGCCGACCACTTACATTAATGCACCATTTGTTGGCGTTTTGGCATACTTTCTGGCTAATCCAAACCCTAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0007275 GO:0008150 GO:0009664 GO:0009791 GO:0009827 GO:0009828 GO:0009900 GO:0009987 GO:0010047 GO:0010154 GO:0016043 GO:0022414 GO:0032501 GO:0032502 GO:0032504 GO:0042545 GO:0045229 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0061458 GO:0071554 GO:0071555 GO:0071669 GO:0071840 GO:1990059

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glyco_hydro_9

Protein Analysis

500

Amino Acids

54.88

Weight (kDa)

4.84

Isoelectric Point (pI)

46.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_9	PF00759	33 - 489	4.8e-159	Glycosyl hydrolase family 9

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004434)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G39010 AT4G39010
fragaria_vesca	FvH4_2g10330
malus_domestica	MD10G1063800.v1.1 MD10G1186600.v1.1
prunus_persica	Prupe.1G331000_v2.0.a1 Prupe.8G085400_v2.0.a1
pyrus_communis	pycom10g04930
rosa_chinensis	RchiOBHm_Chr6g0267611 RchiOBHm_Chr6g0267661 RchiOBHm_Chr6g0267791 RchiOBHm_Chr6g0267801
rosa_laevigata	RLG00000013979 RLG00000013983
rosa_multiflora	Rmu_sc0001833.1_g000001 Rmu_sc0005400.1_g000030
rosa_roxburghii	Rroxscaffold_7G00200500 Rroxscaffold_7G00200540
rosa_rugosa	Rorug06G0033900 Rorug06G0041700.1 Rorug06G0041800
rosa_samantha	Rh6AG155700 Rh6AG155900 Rh6AG156000 Rh6BG156900 Rh6BG157000 Rh6CG152500
rosa_wichuraiana	Rw6G013370 Rw6G013400 Rw6G013520 Rw6G013530 Rw6G013940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	527
AasI	GACNNNNNNGTC	1 cut(s)	1102
Acc36I	ACCTGC	2 cut(s)	418, 527
AccB1I	GGYRCC	2 cut(s)	821, 1358
AccB7I	CCANNNNNTGG	1 cut(s)	1457
AccII	CGCG	1 cut(s)	1079
AccIII	TCCGGA	1 cut(s)	967
AciI	CCGC	7 cut(s)	234, 370, 522, 551, 1005, 1116, 1235
AclWI	GGATC	1 cut(s)	584
AcsI	RAATTY	1 cut(s)	842
AcuI	CTGAAG	2 cut(s)	492, 1314
AcyI	GRCGYC	1 cut(s)	246
AfaI	GTAC	5 cut(s)	215, 599, 914, 995, 1319
AfiI	CCNNNNNNNGG	2 cut(s)	594, 1457
AflIII	ACRYGT	1 cut(s)	909
AgsI	TTSAA	2 cut(s)	330, 1324
AjnI	CCWGG	2 cut(s)	592, 1375
AleI	CACNNNNGTG	2 cut(s)	657, 1251
AluBI	AGCT	5 cut(s)	67, 75, 346, 563, 948
AluI	AGCT	5 cut(s)	67, 75, 346, 563, 948
Alw21I	GWGCWC	2 cut(s)	69, 959
Alw26I	GTCTC	3 cut(s)	408, 536, 889
Alw44I	GTGCAC	1 cut(s)	955
AlwI	GGATC	1 cut(s)	584
Aor13HI	TCCGGA	1 cut(s)	967
AoxI	GGCC	6 cut(s)	117, 375, 552, 630, 868, 1333
ApaLI	GTGCAC	1 cut(s)	955
ApeKI	GCWGC	5 cut(s)	548, 560, 749, 1029, 1039
ApoI	RAATTY	1 cut(s)	842
AseI	ATTAAT	1 cut(s)	1443
AspLEI	GCGC	1 cut(s)	171
AspS9I	GGNCC	7 cut(s)	163, 375, 553, 1095, 1279, 1334, 1373
AsuC2I	CCSGG	2 cut(s)	1002, 1023
AsuHPI	GGTGA	6 cut(s)	263, 446, 551, 1196, 1235, 1238
AvaII	GGWCC	4 cut(s)	163, 1095, 1279, 1373
AxyI	CCTNAGG	1 cut(s)	159
BaeGI	GKGCMC	2 cut(s)	959, 1363
BanI	GGYRCC	2 cut(s)	821, 1358
BanII	GRGCYC	1 cut(s)	69
BauI	CACGAG	2 cut(s)	359, 1364
BbsI	GAAGAC	2 cut(s)	480, 1389
Bbv12I	GWGCWC	2 cut(s)	69, 959
BbvCI	CCTCAGC	1 cut(s)	347
BbvI	GCAGC	5 cut(s)	560, 572, 761, 1041, 1051
BccI	CCATC	7 cut(s)	271, 565, 683, 713, 770, 1061, 1276
BcgI	CGANNNNNNTGC	4 cut(s)	277, 311, 549, 583
BciT130I	CCWGG	2 cut(s)	594, 1377
BcnI	CCSGG	2 cut(s)	1002, 1023
BcoDI	GTCTC	3 cut(s)	408, 536, 889
BfaI	CTAG	5 cut(s)	50, 489, 557, 788, 881
BfoI	RGCGCY	1 cut(s)	172
BfuAI	ACCTGC	2 cut(s)	418, 527
BglI	GCCNNNNNGGC	1 cut(s)	629
BisI	GCNGC	8 cut(s)	235, 549, 552, 561, 750, 1030, 1040, 1117
BlsI	GCNGC	8 cut(s)	236, 550, 553, 562, 751, 1031, 1041, 1118
Bme1390I	CCNGG	4 cut(s)	594, 1002, 1023, 1377
Bme18I	GGWCC	4 cut(s)	163, 1095, 1279, 1373
BmgT120I	GGNCC	7 cut(s)	163, 375, 553, 1095, 1279, 1334, 1373
BmiI	GGNNCC	2 cut(s)	823, 1360
BmrFI	CCNGG	4 cut(s)	594, 1002, 1023, 1377
BmsI	GCATC	1 cut(s)	89
BpiI	GAAGAC	2 cut(s)	480, 1389
BplI	GAGNNNNNCTC	2 cut(s)	340, 372
Bpu10I	CCTNAGC	1 cut(s)	347
BpuEI	CTTGAG	2 cut(s)	377, 756
BpuMI	CCSGG	2 cut(s)	1002, 1023
BsaBI	GATNNNNATC	1 cut(s)	723
BsaHI	GRCGYC	1 cut(s)	246
BsaJI	CCNNGG	5 cut(s)	114, 378, 593, 624, 1252
BsaWI	WCCGGW	2 cut(s)	414, 967
Bsc4I	CCNNNNNNNGG	2 cut(s)	594, 1457
Bse118I	RCCGGY	2 cut(s)	230, 248
Bse21I	CCTNAGG	1 cut(s)	159
Bse3DI	GCAATG	1 cut(s)	1293
Bse8I	GATNNNNATC	1 cut(s)	723
BseAI	TCCGGA	1 cut(s)	967
BseBI	CCWGG	2 cut(s)	594, 1377
BseDI	CCNNGG	5 cut(s)	114, 378, 593, 624, 1252
BseGI	GGATG	3 cut(s)	584, 980, 1405
BseJI	GATNNNNATC	1 cut(s)	723
BseLI	CCNNNNNNNGG	2 cut(s)	594, 1457
BseMI	GCAATG	1 cut(s)	1293
BseMII	CTCAG	6 cut(s)	173, 211, 338, 419, 1326, 1416
BseSI	GKGCMC	2 cut(s)	959, 1363
BseXI	GCAGC	5 cut(s)	560, 572, 761, 1041, 1051
BseYI	CCCAGC	1 cut(s)	372
BsgI	GTGCAG	2 cut(s)	446, 1428
Bsh1236I	CGCG	1 cut(s)	1079
BshFI	GGCC	6 cut(s)	119, 377, 554, 632, 870, 1335
BshNI	GGYRCC	2 cut(s)	821, 1358
BsiHKAI	GWGCWC	2 cut(s)	69, 959
BsiSI	CCGG	6 cut(s)	231, 249, 415, 968, 1002, 1022
BslFI	GGGAC	2 cut(s)	866, 1090
BslI	CCNNNNNNNGG	2 cut(s)	594, 1457
BsmAI	GTCTC	3 cut(s)	408, 536, 889
BsmFI	GGGAC	2 cut(s)	866, 1090
BsmI	GAATGC	2 cut(s)	575, 1242
BsnI	GGCC	6 cut(s)	119, 377, 554, 632, 870, 1335
Bsp1286I	GDGCHC	3 cut(s)	69, 959, 1363
Bsp13I	TCCGGA	1 cut(s)	967
Bsp1407I	TGTACA	1 cut(s)	912
Bsp143I	GATC	3 cut(s)	581, 589, 1402
BspACI	CCGC	7 cut(s)	234, 370, 522, 551, 1005, 1116, 1235
BspANI	GGCC	6 cut(s)	119, 377, 554, 632, 870, 1335
BspCNI	CTCAG	6 cut(s)	172, 210, 339, 418, 1325, 1417
BspEI	TCCGGA	1 cut(s)	967
BspFNI	CGCG	1 cut(s)	1079
BspLI	GGNNCC	2 cut(s)	823, 1360
BspMI	ACCTGC	2 cut(s)	418, 527
BspPI	GGATC	1 cut(s)	584
BspQI	GCTCTTC	1 cut(s)	1264
BspT107I	GGYRCC	2 cut(s)	821, 1358
BsrDI	GCAATG	1 cut(s)	1293
BsrFI	RCCGGY	2 cut(s)	230, 248
BsrGI	TGTACA	1 cut(s)	912
BssAI	RCCGGY	2 cut(s)	230, 248
BssECI	CCNNGG	5 cut(s)	114, 378, 593, 624, 1252
BssMI	GATC	3 cut(s)	581, 589, 1402
BssNI	GRCGYC	1 cut(s)	246
BssSI	CACGAG	2 cut(s)	359, 1364
BssT1I	CCWWGG	2 cut(s)	114, 624
Bst2BI	CACGAG	2 cut(s)	359, 1364
Bst2UI	CCWGG	2 cut(s)	594, 1377
Bst4CI	ACNGT	4 cut(s)	400, 458, 496, 1253
Bst6I	CTCTTC	2 cut(s)	1264, 1333
BstACI	GRCGYC	1 cut(s)	246
BstAUI	TGTACA	1 cut(s)	912
BstC8I	GCNNGC	5 cut(s)	183, 300, 864, 868, 1300
BstDEI	CTNAG	7 cut(s)	159, 197, 347, 405, 898, 1312, 1425
BstDSI	CCRYGG	2 cut(s)	378, 1252
BstEII	GGTNACC	3 cut(s)	434, 782, 1223
BstF5I	GGATG	3 cut(s)	584, 980, 1405
BstFNI	CGCG	1 cut(s)	1079
BstH2I	RGCGCY	1 cut(s)	172
BstHHI	GCGC	1 cut(s)	171
BstKTI	GATC	3 cut(s)	584, 592, 1405
BstMAI	GTCTC	3 cut(s)	408, 536, 889
BstMBI	GATC	3 cut(s)	581, 589, 1402
BstMWI	GCNNNNNNNGC	6 cut(s)	362, 557, 560, 629, 954, 1308
BstNI	CCWGG	2 cut(s)	594, 1377
BstNSI	RCATGY	3 cut(s)	866, 913, 1046
BstPI	GGTNACC	3 cut(s)	434, 782, 1223
BstSCI	CCNGG	4 cut(s)	592, 1000, 1021, 1375
BstSLI	GKGCMC	2 cut(s)	959, 1363
BstUI	CGCG	1 cut(s)	1079
BstV1I	GCAGC	5 cut(s)	560, 572, 761, 1041, 1051
BstV2I	GAAGAC	2 cut(s)	480, 1389
Bsu36I	CCTNAGG	1 cut(s)	159
BsuRI	GGCC	6 cut(s)	119, 377, 554, 632, 870, 1335
BtgI	CCRYGG	2 cut(s)	378, 1252
BtsCI	GGATG	3 cut(s)	584, 980, 1405
BtsIMutI	CAGTG	2 cut(s)	205, 1320
BveI	ACCTGC	2 cut(s)	418, 527
Cac8I	GCNNGC	5 cut(s)	183, 300, 864, 868, 1300
CfoI	GCGC	1 cut(s)	171
Cfr10I	RCCGGY	2 cut(s)	230, 248
Cfr13I	GGNCC	7 cut(s)	163, 375, 553, 1095, 1279, 1334, 1373
CseI	GACGC	1 cut(s)	254
Csp6I	GTAC	5 cut(s)	214, 598, 913, 994, 1318
CviAII	CATG	9 cut(s)	92, 177, 478, 753, 863, 910, 952, 1043, 1210
CviQI	GTAC	5 cut(s)	214, 598, 913, 994, 1318
DdeI	CTNAG	7 cut(s)	159, 197, 347, 405, 898, 1312, 1425
DpnI	GATC	3 cut(s)	583, 591, 1404
DpnII	GATC	3 cut(s)	581, 589, 1402
DrdI	GACNNNNNNGTC	1 cut(s)	1102
DseDI	GACNNNNNNGTC	1 cut(s)	1102
Eam1104I	CTCTTC	2 cut(s)	1264, 1333
EarI	CTCTTC	2 cut(s)	1264, 1333
EciI	GGCGGA	1 cut(s)	1020
Ecl136II	GAGCTC	1 cut(s)	67
Eco130I	CCWWGG	2 cut(s)	114, 624
Eco147I	AGGCCT	1 cut(s)	632
Eco24I	GRGCYC	1 cut(s)	69
Eco47I	GGWCC	4 cut(s)	163, 1095, 1279, 1373
Eco53kI	GAGCTC	1 cut(s)	67
Eco57I	CTGAAG	2 cut(s)	492, 1314
Eco81I	CCTNAGG	1 cut(s)	159
Eco91I	GGTNACC	3 cut(s)	434, 782, 1223
EcoICRI	GAGCTC	1 cut(s)	67
EcoO109I	RGGNCCY	1 cut(s)	1334
EcoO65I	GGTNACC	3 cut(s)	434, 782, 1223
EcoRII	CCWGG	2 cut(s)	592, 1375
EcoT14I	CCWWGG	2 cut(s)	114, 624
EcoT38I	GRGCYC	1 cut(s)	69
ErhI	CCWWGG	2 cut(s)	114, 624
FaeI	CATG	9 cut(s)	95, 180, 481, 756, 866, 913, 955, 1046, 1213
FalI	AAGNNNNNCTT	4 cut(s)	617, 649, 881, 913
FaqI	GGGAC	2 cut(s)	866, 1090
FatI	CATG	9 cut(s)	91, 176, 477, 752, 862, 909, 951, 1042, 1209
FauI	CCCGC	1 cut(s)	529
Fnu4HI	GCNGC	8 cut(s)	235, 549, 552, 561, 750, 1030, 1040, 1117
FokI	GGATG	3 cut(s)	571, 967, 1412
FriOI	GRGCYC	1 cut(s)	69
Fsp4HI	GCNGC	8 cut(s)	235, 549, 552, 561, 750, 1030, 1040, 1117
FspBI	CTAG	5 cut(s)	50, 489, 557, 788, 881
GlaI	GCGC	1 cut(s)	170
GluI	GCNGC	8 cut(s)	235, 549, 552, 561, 750, 1030, 1040, 1117
GsaI	CCCAGC	1 cut(s)	376
HaeII	RGCGCY	1 cut(s)	172
HaeIII	GGCC	6 cut(s)	119, 377, 554, 632, 870, 1335
HapII	CCGG	6 cut(s)	231, 249, 415, 968, 1002, 1022
HgaI	GACGC	1 cut(s)	254
HhaI	GCGC	1 cut(s)	171
Hin1I	GRCGYC	1 cut(s)	246
Hin1II	CATG	9 cut(s)	95, 180, 481, 756, 866, 913, 955, 1046, 1213
Hin6I	GCGC	1 cut(s)	169
HinP1I	GCGC	1 cut(s)	169
HindIII	AAGCTT	1 cut(s)	73
HinfI	GANTC	6 cut(s)	195, 326, 509, 923, 971, 1008
HpaII	CCGG	6 cut(s)	231, 249, 415, 968, 1002, 1022
HphI	GGTGA	6 cut(s)	263, 446, 551, 1196, 1235, 1238
Hpy166II	GTNNAC	6 cut(s)	210, 285, 957, 996, 1075, 1318
Hpy188I	TCNGA	6 cut(s)	276, 529, 581, 589, 604, 1426
Hpy188III	TCNNGA	8 cut(s)	161, 192, 286, 394, 728, 773, 920, 968
Hpy8I	GTNNAC	6 cut(s)	210, 285, 957, 996, 1075, 1318
Hpy99I	CGWCG	3 cut(s)	248, 292, 571
HpyAV	CCTTC	3 cut(s)	28, 896, 1301
HpyCH4III	ACNGT	4 cut(s)	400, 458, 496, 1253
HpyCH4IV	ACGT	3 cut(s)	212, 959, 1128
HpyCH4V	TGCA	7 cut(s)	80, 427, 957, 1039, 1302, 1409, 1448
HpyF10VI	GCNNNNNNNGC	6 cut(s)	362, 557, 560, 629, 954, 1308
HpyF3I	CTNAG	7 cut(s)	159, 197, 347, 405, 898, 1312, 1425
HpySE526I	ACGT	3 cut(s)	212, 959, 1128
Hsp92I	GRCGYC	1 cut(s)	246
Hsp92II	CATG	9 cut(s)	95, 180, 481, 756, 866, 913, 955, 1046, 1213
HspAI	GCGC	1 cut(s)	169
Kpn2I	TCCGGA	1 cut(s)	967
Kzo9I	GATC	3 cut(s)	581, 589, 1402
LguI	GCTCTTC	1 cut(s)	1264
LmnI	GCTCC	1 cut(s)	72
Lsp1109I	GCAGC	5 cut(s)	560, 572, 761, 1041, 1051
LweI	GCATC	1 cut(s)	89
MaeI	CTAG	5 cut(s)	50, 489, 557, 788, 881
MaeII	ACGT	3 cut(s)	212, 959, 1128
MaeIII	GTNAC	6 cut(s)	200, 434, 766, 782, 1045, 1223
MalI	GATC	3 cut(s)	583, 591, 1404
MboI	GATC	3 cut(s)	581, 589, 1402
MboII	GAAGA	5 cut(s)	121, 485, 1251, 1320, 1394
MhlI	GDGCHC	3 cut(s)	69, 959, 1363
MluCI	AATT	8 cut(s)	187, 441, 673, 842, 963, 1033, 1081, 1495
MlyI	GAGTC	3 cut(s)	189, 965, 1002
MroI	TCCGGA	1 cut(s)	967
MseI	TTAA	5 cut(s)	614, 684, 962, 1416, 1443
MslI	CAYNNNNRTG	2 cut(s)	657, 1251
MspA1I	CMGCKG	2 cut(s)	372, 551
MspI	CCGG	6 cut(s)	231, 249, 415, 968, 1002, 1022
MspR9I	CCNGG	4 cut(s)	594, 1002, 1023, 1377
Mva1269I	GAATGC	2 cut(s)	575, 1242
MvaI	CCWGG	2 cut(s)	594, 1377
MvnI	CGCG	1 cut(s)	1079
MwoI	GCNNNNNNNGC	6 cut(s)	362, 557, 560, 629, 954, 1308
NciI	CCSGG	2 cut(s)	1002, 1023
NdeII	GATC	3 cut(s)	581, 589, 1402
NlaIII	CATG	9 cut(s)	95, 180, 481, 756, 866, 913, 955, 1046, 1213
NlaIV	GGNNCC	2 cut(s)	823, 1360
NmuCI	GTSAC	4 cut(s)	200, 434, 766, 1223
NspI	RCATGY	3 cut(s)	866, 913, 1046
OliI	CACNNNNGTG	2 cut(s)	657, 1251
PaeI	GCATGC	1 cut(s)	866
PaqCI	CACCTGC	1 cut(s)	527
PceI	AGGCCT	1 cut(s)	632
PciI	ACATGT	1 cut(s)	909
PciSI	GCTCTTC	1 cut(s)	1264
PctI	GAATGC	2 cut(s)	575, 1242
PfeI	GAWTC	3 cut(s)	326, 509, 923
PflFI	GACNNNGTC	1 cut(s)	400
PflMI	CCANNNNNTGG	1 cut(s)	1457
PfoI	TCCNGGA	1 cut(s)	1375
PkrI	GCNGC	8 cut(s)	236, 550, 553, 562, 751, 1031, 1041, 1118
PleI	GAGTC	3 cut(s)	189, 965, 1002
PpsI	GAGTC	3 cut(s)	189, 965, 1002
PscI	ACATGT	1 cut(s)	909
PshBI	ATTAAT	1 cut(s)	1443
Psp124BI	GAGCTC	1 cut(s)	69
Psp6I	CCWGG	2 cut(s)	592, 1375
PspEI	GGTNACC	3 cut(s)	434, 782, 1223
PspFI	CCCAGC	1 cut(s)	372
PspGI	CCWGG	2 cut(s)	592, 1375
PspN4I	GGNNCC	2 cut(s)	823, 1360
PspPI	GGNCC	7 cut(s)	163, 375, 553, 1095, 1279, 1334, 1373
PsyI	GACNNNGTC	1 cut(s)	400
RsaI	GTAC	5 cut(s)	215, 599, 914, 995, 1319
RsaNI	GTAC	5 cut(s)	214, 598, 913, 994, 1318
RseI	CAYNNNNRTG	2 cut(s)	657, 1251
SacI	GAGCTC	1 cut(s)	69
SapI	GCTCTTC	1 cut(s)	1264
SaqAI	TTAA	5 cut(s)	614, 684, 962, 1416, 1443
SatI	GCNGC	8 cut(s)	235, 549, 552, 561, 750, 1030, 1040, 1117
Sau3AI	GATC	3 cut(s)	581, 589, 1402
Sau96I	GGNCC	7 cut(s)	163, 375, 553, 1095, 1279, 1334, 1373
SchI	GAGTC	3 cut(s)	189, 965, 1002
ScrFI	CCNGG	4 cut(s)	594, 1002, 1023, 1377
SduI	GDGCHC	3 cut(s)	69, 959, 1363
SfaNI	GCATC	1 cut(s)	89
SgrAI	CRCCGGYG	1 cut(s)	248
SinI	GGWCC	4 cut(s)	163, 1095, 1279, 1373
SmiMI	CAYNNNNRTG	2 cut(s)	657, 1251
SmlI	CTYRAG	2 cut(s)	392, 771
SmoI	CTYRAG	2 cut(s)	392, 771
SphI	GCATGC	1 cut(s)	866
Sse9I	AATT	8 cut(s)	187, 441, 673, 842, 963, 1033, 1081, 1495
SseBI	AGGCCT	1 cut(s)	632
SsiI	CCGC	7 cut(s)	234, 370, 522, 551, 1005, 1116, 1235
SspMI	CTAG	5 cut(s)	50, 489, 557, 788, 881
SstI	GAGCTC	1 cut(s)	69
StuI	AGGCCT	1 cut(s)	632
StyD4I	CCNGG	4 cut(s)	592, 1000, 1021, 1375
StyI	CCWWGG	2 cut(s)	114, 624
TaaI	ACNGT	4 cut(s)	400, 458, 496, 1253
TaiI	ACGT	3 cut(s)	215, 962, 1131
TaqI	TCGA	5 cut(s)	63, 132, 569, 921, 990
TasI	AATT	8 cut(s)	187, 441, 673, 842, 963, 1033, 1081, 1495
TatI	WGTACW	3 cut(s)	912, 993, 1317
TauI	GCSGC	3 cut(s)	237, 554, 1119
TfiI	GAWTC	3 cut(s)	326, 509, 923
Tru1I	TTAA	5 cut(s)	614, 684, 962, 1416, 1443
Tru9I	TTAA	5 cut(s)	614, 684, 962, 1416, 1443
TscAI	CASTG	2 cut(s)	205, 1320
TseFI	GTSAC	4 cut(s)	200, 434, 766, 1223
TseI	GCWGC	5 cut(s)	548, 560, 749, 1029, 1039
Tsp45I	GTSAC	4 cut(s)	200, 434, 766, 1223
TspDTI	ATGAA	2 cut(s)	17, 940
TspGWI	ACGGA	3 cut(s)	28, 395, 1108
TspRI	CASTG	2 cut(s)	205, 1320
Tth111I	GACNNNGTC	1 cut(s)	400
Van91I	CCANNNNNTGG	1 cut(s)	1457
VneI	GTGCAC	1 cut(s)	955
VpaK11BI	GGWCC	4 cut(s)	163, 1095, 1279, 1373
VspI	ATTAAT	1 cut(s)	1443
XapI	RAATTY	1 cut(s)	842
XceI	RCATGY	3 cut(s)	866, 913, 1046
XspI	CTAG	5 cut(s)	50, 489, 557, 788, 881

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.