Rw6G013520

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0004434
Physical Location & Seq
Forward (+)
25022159 .. 25026110
3952 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G013520.1

Sequence Viewer

Length: 1485 bp
ATGAAACCCATGACTCATTTCCTCTCCCTCCTTCTTCTCCTACTCTGCCTCCTCGCCACCGTGCACGGCGGAGGGCACGACTACCAGGACGCATTGTCGAAATGCATACTCTTTTTTGAGGGCCAGAGGTCGGGGGTTCTGCCTCAGGACCAACGCCAGTCGTGGCGGGCTAATTCCGGGTTGAGTGATGGCTGGACTTACAACACTGACCTCACCGGCGGCTACTACGACGCAGGAGACAACGTCAAGTTTGGGTTCCCGATGGCCTTCACGACCACAATGCTGGCGTGGAGTGTCATCGAGTTTGGGGACTCCATGCCTCCCAATGAGCACAGGAATGCGCTGGTTGCCCTTCGTTGGGGGACGGATTATCTCCTCAAAACGGTGTCGCAGCCCAATCGGATTTTCGTACAAGTCGGGGATCCAATTATAGACCACAATTGCTGGGAAAGACCAGAAGATATGGACACAGCCAGGACTGCATATGCCGTGGACTCGCCGAATCCAGCTTCTGATGTTGCCGGTGAGACAGCTGCAGCGCTGTCAGCTTCCTCAATGGCATTCCAATCCTCTGACCCTGGTTACGCAGACACATTGTTGCGAAATGCCATGAAGGCCTTCGAGTTTGCTAATACTTACAGAGGAGCTTACAGTGATAATGCCAATGTTAGAGATGGTGTCTGCCCCTTTTACTGTGATTTCGATGGTTACCAAGACGAATTGCTATGGAGTGCAGCCTGGCTGAGGAGAGTCACTCAGGATGGTGGTTACCTTGACTACATACAAAACAATGGCAAAACTCTTGGTGCAGAGGACAATATAAACGAATTTGGTTGGGACAACAAGCATGCTGGCCTCAATGTTCTAATCTCCAAGGAAGTCCTGGAAGGCAACATGTACAACCTTGAATCATACAAATCATCGGCTGATAGCTTCATGTGCACATTAATACCGGAGTCGTCATCCTCCCACATCGAGTACACTCCAGCCGGTCTCATCTACAAGCCAGGAGGCAGCAATCTGCAACATGTAACTTCGATTTCATTCCTACTACTAGTTTACGCCAATTACCTTGCACGAACCTCGCGATCCCTCAGCTGTGGTAACATAGATGTCAGTCCAACAACGCTTCGCCAAATCGCCAAAAAGCAGGTTGATTACATTTTGGGTGATAATCCAATGGGTTTGTCATACATGGTCGGATACGGCGACCATTATCCGCAGCGAATTCACCATCGTGGATCTTCTTTACCATCTATCAAGGATCATCCCCAACCCATTGCTTGCAAGGAAGGCTCAGTGTACTTCAATTCGTCAAACCCTAACCCTAATCCCCTTGTGGGAGCTCTAGTAGGAGGACCTGGAGAAGATGATGTCTATGAGGATGATCGCGCTGATTTTAGGAAGTCCGAACCGACCACATACATTAATGCACCATTTGTTGGTGTTTTGGCATACTTTGTGGCTAATCCCAGTCCAAACTAG

Protein Analysis

494

Amino Acids

54.48

Weight (kDa)

4.88

Isoelectric Point (pI)

48.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 28 - 484 6.7e-157 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1141
AccB7I CCANNNNNTGG 1 cut(s) 1442
AccII CGCG 2 cut(s) 1087, 1392
AciI CCGC 4 cut(s) 69, 166, 219, 1220
AclWI GGATC 5 cut(s) 416, 429, 1083, 1249, 1272
AcsI RAATTY 2 cut(s) 827, 1227
AfaI GTAC 4 cut(s) 411, 899, 980, 1304
AfeI AGCGCT 1 cut(s) 540
AfiI CCNNNNNNNGG 7 cut(s) 130, 357, 358, 382, 744, 1340, 1442
AflIII ACRYGT 2 cut(s) 894, 1027
AgsI TTSAA 2 cut(s) 908, 1309
AhdI GACNNNNNGTC 1 cut(s) 94
AhlI ACTAGT 1 cut(s) 1054
AjnI CCWGG 7 cut(s) 84, 473, 577, 737, 882, 1006, 1360
AjuI GAANNNNNNNTTGG 2 cut(s) 389, 421
AleI CACNNNNGTG 1 cut(s) 1236
AluBI AGCT 7 cut(s) 509, 533, 548, 647, 933, 1098, 1346
AluI AGCT 7 cut(s) 509, 533, 548, 647, 933, 1098, 1346
Alw21I GWGCWC 4 cut(s) 66, 333, 944, 1348
Alw26I GTCTC 3 cut(s) 231, 521, 998
Alw44I GTGCAC 2 cut(s) 62, 940
AlwI GGATC 5 cut(s) 416, 429, 1083, 1249, 1272
AlwNI CAGNNNCTG 1 cut(s) 512
Aor51HI AGCGCT 1 cut(s) 540
AoxI GGCC 4 cut(s) 121, 264, 615, 853
ApaLI GTGCAC 2 cut(s) 62, 940
ApeKI GCWGC 6 cut(s) 391, 533, 536, 734, 1014, 1222
ApoI RAATTY 2 cut(s) 827, 1227
AseI ATTAAT 2 cut(s) 947, 1428
Asp700I GAANNNNTTC 1 cut(s) 617
AspLEI GCGC 3 cut(s) 343, 541, 1394
AspS9I GGNCC 3 cut(s) 121, 148, 1358
AsuC2I CCSGG 1 cut(s) 178
AsuHPI GGTGA 4 cut(s) 205, 536, 1181, 1223
AvaII GGWCC 2 cut(s) 148, 1358
AxyI CCTNAGG 1 cut(s) 144
BaeGI GKGCMC 3 cut(s) 66, 78, 944
BamHI GGATCC 1 cut(s) 421
BanII GRGCYC 1 cut(s) 1348
Bbv12I GWGCWC 4 cut(s) 66, 333, 944, 1348
BbvCI CCTCAGC 2 cut(s) 743, 1094
BbvI GCAGC 6 cut(s) 403, 520, 548, 746, 1026, 1234
BccI CCATC 7 cut(s) 182, 256, 668, 698, 755, 1242, 1261
BceAI ACGGC 3 cut(s) 82, 473, 1222
BcgI CGANNNNNNTGC 6 cut(s) 262, 296, 380, 414, 1065, 1099
BciT130I CCWGG 7 cut(s) 86, 475, 579, 739, 884, 1008, 1362
BciVI GTATCC 1 cut(s) 1196
BcnI CCSGG 1 cut(s) 178
BcoDI GTCTC 3 cut(s) 231, 521, 998
BcuI ACTAGT 1 cut(s) 1054
BfaI CTAG 3 cut(s) 1055, 1349, 1483
BfmI CTRYAG 1 cut(s) 534
BfoI RGCGCY 1 cut(s) 542
BfuAI ACCTGC 1 cut(s) 1141
BfuI GTATCC 1 cut(s) 1196
BglI GCCNNNNNGGC 1 cut(s) 614
BisI GCNGC 7 cut(s) 220, 392, 534, 537, 735, 1015, 1223
BlsI GCNGC 7 cut(s) 221, 393, 535, 538, 736, 1016, 1224
Bme1390I CCNGG 8 cut(s) 86, 178, 475, 579, 739, 884, 1008, 1362
Bme18I GGWCC 2 cut(s) 148, 1358
BmeRI GACNNNNNGTC 1 cut(s) 94
BmgT120I GGNCC 3 cut(s) 121, 148, 1358
BmiI GGNNCC 2 cut(s) 257, 423
BmrFI CCNGG 8 cut(s) 86, 178, 475, 579, 739, 884, 1008, 1362
BmrI ACTGGG 1 cut(s) 1467
BmuI ACTGGG 1 cut(s) 1467
BplI GAGNNNNNCTC 2 cut(s) 739, 771
BpmI CTGGAG 2 cut(s) 969, 1383
Bpu10I CCTNAGC 2 cut(s) 743, 1094
BpuMI CCSGG 1 cut(s) 178
BsaI GGTCTC 1 cut(s) 998
BsaJI CCNNGG 3 cut(s) 489, 577, 873
BsaWI WCCGGW 1 cut(s) 952
BsaXI ACNNNNNCTCC 6 cut(s) 33, 63, 228, 258, 1335, 1365
Bsc4I CCNNNNNNNGG 7 cut(s) 130, 357, 358, 382, 744, 1340, 1442
Bse118I RCCGGY 3 cut(s) 215, 521, 989
Bse1I ACTGG 2 cut(s) 157, 1473
Bse21I CCTNAGG 1 cut(s) 144
Bse3DI GCAATG 1 cut(s) 1278
BseBI CCWGG 7 cut(s) 86, 475, 579, 739, 884, 1008, 1362
BseDI CCNNGG 3 cut(s) 489, 577, 873
BseGI GGATG 4 cut(s) 766, 962, 1267, 1390
BseLI CCNNNNNNNGG 7 cut(s) 130, 357, 358, 382, 744, 1340, 1442
BseMI GCAATG 1 cut(s) 1278
BseMII CTCAG 5 cut(s) 158, 734, 770, 1108, 1311
BseNI ACTGG 2 cut(s) 157, 1473
BseRI GAGGAG 4 cut(s) 41, 365, 657, 760
BseSI GKGCMC 3 cut(s) 66, 78, 944
BseXI GCAGC 6 cut(s) 403, 520, 548, 746, 1026, 1234
BseYI CCCAGC 1 cut(s) 444
BsgI GTGCAG 2 cut(s) 753, 828
Bsh1236I CGCG 2 cut(s) 1087, 1392
BshFI GGCC 4 cut(s) 123, 266, 617, 855
BsiHKAI GWGCWC 4 cut(s) 66, 333, 944, 1348
BsiSI CCGG 5 cut(s) 177, 216, 522, 953, 990
BslFI GGGAC 3 cut(s) 323, 376, 851
BslI CCNNNNNNNGG 7 cut(s) 130, 357, 358, 382, 744, 1340, 1442
BsmAI GTCTC 3 cut(s) 231, 521, 998
BsmFI GGGAC 3 cut(s) 323, 376, 851
BsmI GAATGC 2 cut(s) 343, 560
BsnI GGCC 4 cut(s) 123, 266, 617, 855
Bso31I GGTCTC 1 cut(s) 998
Bsp1286I GDGCHC 5 cut(s) 66, 78, 333, 944, 1348
Bsp1407I TGTACA 1 cut(s) 897
Bsp143I GATC 5 cut(s) 421, 1088, 1241, 1264, 1387
Bsp68I TCGCGA 1 cut(s) 1087
BspACI CCGC 4 cut(s) 69, 166, 219, 1220
BspANI GGCC 4 cut(s) 123, 266, 617, 855
BspCNI CTCAG 5 cut(s) 157, 735, 769, 1107, 1310
BspFNI CGCG 2 cut(s) 1087, 1392
BspLI GGNNCC 2 cut(s) 257, 423
BspMAI CTGCAG 1 cut(s) 538
BspMI ACCTGC 1 cut(s) 1141
BspPI GGATC 5 cut(s) 416, 429, 1083, 1249, 1272
BspTNI GGTCTC 1 cut(s) 998
BsrDI GCAATG 1 cut(s) 1278
BsrFI RCCGGY 3 cut(s) 215, 521, 989
BsrGI TGTACA 1 cut(s) 897
BsrI ACTGG 2 cut(s) 157, 1473
BssAI RCCGGY 3 cut(s) 215, 521, 989
BssECI CCNNGG 3 cut(s) 489, 577, 873
BssMI GATC 5 cut(s) 421, 1088, 1241, 1264, 1387
BssT1I CCWWGG 1 cut(s) 873
Bst2UI CCWGG 7 cut(s) 86, 475, 579, 739, 884, 1008, 1362
Bst4CI ACNGT 4 cut(s) 61, 385, 653, 695
BstAUI TGTACA 1 cut(s) 897
BstC8I GCNNGC 5 cut(s) 168, 285, 849, 853, 1285
BstDEI CTNAG 5 cut(s) 144, 743, 756, 1094, 1297
BstDSI CCRYGG 1 cut(s) 489
BstEII GGTNACC 2 cut(s) 707, 767
BstENI CCTNNNNNAGG 1 cut(s) 742
BstF5I GGATG 4 cut(s) 766, 962, 1267, 1390
BstFNI CGCG 2 cut(s) 1087, 1392
BstH2I RGCGCY 1 cut(s) 542
BstHHI GCGC 3 cut(s) 343, 541, 1394
BstKTI GATC 5 cut(s) 424, 1091, 1244, 1267, 1390
BstMAI GTCTC 3 cut(s) 231, 521, 998
BstMBI GATC 5 cut(s) 421, 1088, 1241, 1264, 1387
BstMWI GCNNNNNNNGC 6 cut(s) 347, 479, 545, 614, 939, 1293
BstNI CCWGG 7 cut(s) 86, 475, 579, 739, 884, 1008, 1362
BstNSI RCATGY 3 cut(s) 851, 898, 1031
BstPI GGTNACC 2 cut(s) 707, 767
BstSCI CCNGG 8 cut(s) 84, 176, 473, 577, 737, 882, 1006, 1360
BstSFI CTRYAG 1 cut(s) 534
BstSLI GKGCMC 3 cut(s) 66, 78, 944
BstUI CGCG 2 cut(s) 1087, 1392
BstV1I GCAGC 6 cut(s) 403, 520, 548, 746, 1026, 1234
BstX2I RGATCY 2 cut(s) 421, 1241
BstXI CCANNNNNNTGG 1 cut(s) 283
BstYI RGATCY 2 cut(s) 421, 1241
Bsu36I CCTNAGG 1 cut(s) 144
BsuI GTATCC 1 cut(s) 1196
BsuRI GGCC 4 cut(s) 123, 266, 617, 855
BtgI CCRYGG 1 cut(s) 489
BtsCI GGATG 4 cut(s) 766, 962, 1267, 1390
BtsIMutI CAGTG 3 cut(s) 204, 658, 1305
BtuMI TCGCGA 1 cut(s) 1087
BveI ACCTGC 1 cut(s) 1141
Cac8I GCNNGC 5 cut(s) 168, 285, 849, 853, 1285
CaiI CAGNNNCTG 1 cut(s) 512
CfoI GCGC 3 cut(s) 343, 541, 1394
Cfr10I RCCGGY 3 cut(s) 215, 521, 989
Cfr13I GGNCC 3 cut(s) 121, 148, 1358
CseI GACGC 2 cut(s) 98, 239
Csp6I GTAC 4 cut(s) 410, 898, 979, 1303
CviAII CATG 8 cut(s) 10, 316, 610, 848, 895, 937, 1028, 1195
CviQI GTAC 4 cut(s) 410, 898, 979, 1303
DdeI CTNAG 5 cut(s) 144, 743, 756, 1094, 1297
DpnI GATC 5 cut(s) 423, 1090, 1243, 1266, 1389
DpnII GATC 5 cut(s) 421, 1088, 1241, 1264, 1387
DriI GACNNNNNGTC 1 cut(s) 94
Eam1105I GACNNNNNGTC 1 cut(s) 94
EciI GGCGGA 1 cut(s) 84
Ecl136II GAGCTC 1 cut(s) 1346
Eco130I CCWWGG 1 cut(s) 873
Eco147I AGGCCT 1 cut(s) 617
Eco24I GRGCYC 1 cut(s) 1348
Eco31I GGTCTC 1 cut(s) 998
Eco47I GGWCC 2 cut(s) 148, 1358
Eco47III AGCGCT 1 cut(s) 540
Eco53kI GAGCTC 1 cut(s) 1346
Eco81I CCTNAGG 1 cut(s) 144
Eco91I GGTNACC 2 cut(s) 707, 767
EcoICRI GAGCTC 1 cut(s) 1346
EcoNI CCTNNNNNAGG 1 cut(s) 742
EcoO109I RGGNCCY 1 cut(s) 1358
EcoO65I GGTNACC 2 cut(s) 707, 767
EcoRI GAATTC 1 cut(s) 1227
EcoRII CCWGG 7 cut(s) 84, 473, 577, 737, 882, 1006, 1360
EcoT14I CCWWGG 1 cut(s) 873
EcoT22I ATGCAT 1 cut(s) 107
EcoT38I GRGCYC 1 cut(s) 1348
ErhI CCWWGG 1 cut(s) 873
FaeI CATG 8 cut(s) 13, 319, 613, 851, 898, 940, 1031, 1198
FaqI GGGAC 3 cut(s) 323, 376, 851
FatI CATG 8 cut(s) 9, 315, 609, 847, 894, 936, 1027, 1194
FauI CCCGC 1 cut(s) 159
FauNDI CATATG 1 cut(s) 484
Fnu4HI GCNGC 7 cut(s) 220, 392, 534, 537, 735, 1015, 1223
FokI GGATG 4 cut(s) 773, 949, 1254, 1397
FriOI GRGCYC 1 cut(s) 1348
Fsp4HI GCNGC 7 cut(s) 220, 392, 534, 537, 735, 1015, 1223
FspBI CTAG 3 cut(s) 1055, 1349, 1483
GlaI GCGC 3 cut(s) 342, 540, 1393
GluI GCNGC 7 cut(s) 220, 392, 534, 537, 735, 1015, 1223
GsaI CCCAGC 1 cut(s) 448
GsuI CTGGAG 2 cut(s) 969, 1383
HaeII RGCGCY 1 cut(s) 542
HaeIII GGCC 4 cut(s) 123, 266, 617, 855
HapII CCGG 5 cut(s) 177, 216, 522, 953, 990
HgaI GACGC 2 cut(s) 98, 239
HhaI GCGC 3 cut(s) 343, 541, 1394
Hin1II CATG 8 cut(s) 13, 319, 613, 851, 898, 940, 1031, 1198
Hin6I GCGC 3 cut(s) 341, 539, 1392
HinP1I GCGC 3 cut(s) 341, 539, 1392
HinfI GANTC 7 cut(s) 13, 311, 494, 502, 750, 908, 956
HpaII CCGG 5 cut(s) 177, 216, 522, 953, 990
HphI GGTGA 4 cut(s) 205, 536, 1181, 1223
Hpy166II GTNNAC 6 cut(s) 64, 493, 942, 981, 1060, 1303
Hpy188I TCNGA 5 cut(s) 402, 514, 574, 1202, 1411
Hpy188III TCNNGA 5 cut(s) 146, 259, 271, 758, 1086
Hpy8I GTNNAC 6 cut(s) 64, 493, 942, 981, 1060, 1303
Hpy99I CGWCG 1 cut(s) 233
HpyAV CCTTC 7 cut(s) 41, 277, 362, 607, 628, 881, 1286
HpyCH4III ACNGT 4 cut(s) 61, 385, 653, 695
HpyCH4IV ACGT 1 cut(s) 243
HpyF10VI GCNNNNNNNGC 6 cut(s) 347, 479, 545, 614, 939, 1293
HpyF3I CTNAG 5 cut(s) 144, 743, 756, 1094, 1297
HpySE526I ACGT 1 cut(s) 243
Hsp92II CATG 8 cut(s) 13, 319, 613, 851, 898, 940, 1031, 1198
HspAI GCGC 3 cut(s) 341, 539, 1392
Kzo9I GATC 5 cut(s) 421, 1088, 1241, 1264, 1387
LmnI GCTCC 2 cut(s) 644, 1343
Lsp1109I GCAGC 6 cut(s) 403, 520, 548, 746, 1026, 1234
MaeI CTAG 3 cut(s) 1055, 1349, 1483
MaeII ACGT 1 cut(s) 243
MaeIII GTNAC 6 cut(s) 581, 707, 751, 767, 1030, 1103
MalI GATC 5 cut(s) 423, 1090, 1243, 1266, 1389
MboI GATC 5 cut(s) 421, 1088, 1241, 1264, 1387
MboII GAAGA 4 cut(s) 26, 470, 1236, 1379
MfeI CAATTG 1 cut(s) 439
MflI RGATCY 2 cut(s) 421, 1241
MhlI GDGCHC 5 cut(s) 66, 78, 333, 944, 1348
MluCI AATT 8 cut(s) 172, 426, 439, 719, 827, 1066, 1227, 1309
MlyI GAGTC 5 cut(s) 7, 305, 488, 759, 965
MmeI TCCRAC 2 cut(s) 1145, 1180
Mph1103I ATGCAT 1 cut(s) 107
MroXI GAANNNNTTC 1 cut(s) 617
MseI TTAA 2 cut(s) 947, 1428
MslI CAYNNNNRTG 2 cut(s) 336, 1236
MspA1I CMGCKG 2 cut(s) 533, 1098
MspI CCGG 5 cut(s) 177, 216, 522, 953, 990
MspR9I CCNGG 8 cut(s) 86, 178, 475, 579, 739, 884, 1008, 1362
MunI CAATTG 1 cut(s) 439
Mva1269I GAATGC 2 cut(s) 343, 560
MvaI CCWGG 7 cut(s) 86, 475, 579, 739, 884, 1008, 1362
MvnI CGCG 2 cut(s) 1087, 1392
MwoI GCNNNNNNNGC 6 cut(s) 347, 479, 545, 614, 939, 1293
NciI CCSGG 1 cut(s) 178
NdeI CATATG 1 cut(s) 484
NdeII GATC 5 cut(s) 421, 1088, 1241, 1264, 1387
NlaIII CATG 8 cut(s) 13, 319, 613, 851, 898, 940, 1031, 1198
NlaIV GGNNCC 2 cut(s) 257, 423
NmuCI GTSAC 1 cut(s) 751
NruI TCGCGA 1 cut(s) 1087
NsiI ATGCAT 1 cut(s) 107
NspI RCATGY 3 cut(s) 851, 898, 1031
OliI CACNNNNGTG 1 cut(s) 1236
PaeI GCATGC 1 cut(s) 851
PceI AGGCCT 1 cut(s) 617
PciI ACATGT 2 cut(s) 894, 1027
PcsI WCGNNNNNNNCGW 2 cut(s) 1084, 1206
PctI GAATGC 2 cut(s) 343, 560
PdmI GAANNNNTTC 1 cut(s) 617
PfeI GAWTC 2 cut(s) 502, 908
PflFI GACNNNGTC 1 cut(s) 242
PflMI CCANNNNNTGG 1 cut(s) 1442
PfoI TCCNGGA 1 cut(s) 882
PkrI GCNGC 7 cut(s) 221, 393, 535, 538, 736, 1016, 1224
PleI GAGTC 5 cut(s) 7, 305, 488, 758, 964
PpsI GAGTC 5 cut(s) 7, 305, 488, 758, 964
PpuMI RGGWCCY 1 cut(s) 1358
PscI ACATGT 2 cut(s) 894, 1027
PshBI ATTAAT 2 cut(s) 947, 1428
Psp124BI GAGCTC 1 cut(s) 1348
Psp5II RGGWCCY 1 cut(s) 1358
Psp6I CCWGG 7 cut(s) 84, 473, 577, 737, 882, 1006, 1360
PspEI GGTNACC 2 cut(s) 707, 767
PspFI CCCAGC 1 cut(s) 444
PspGI CCWGG 7 cut(s) 84, 473, 577, 737, 882, 1006, 1360
PspN4I GGNNCC 2 cut(s) 257, 423
PspPI GGNCC 3 cut(s) 121, 148, 1358
PspPPI RGGWCCY 1 cut(s) 1358
PstI CTGCAG 1 cut(s) 538
PstNI CAGNNNCTG 1 cut(s) 512
PsuI RGATCY 2 cut(s) 421, 1241
PsyI GACNNNGTC 1 cut(s) 242
PvuII CAGCTG 2 cut(s) 533, 1098
RruI TCGCGA 1 cut(s) 1087
RsaI GTAC 4 cut(s) 411, 899, 980, 1304
RsaNI GTAC 4 cut(s) 410, 898, 979, 1303
RseI CAYNNNNRTG 2 cut(s) 336, 1236
SacI GAGCTC 1 cut(s) 1348
SaqAI TTAA 2 cut(s) 947, 1428
SatI GCNGC 7 cut(s) 220, 392, 534, 537, 735, 1015, 1223
Sau3AI GATC 5 cut(s) 421, 1088, 1241, 1264, 1387
Sau96I GGNCC 3 cut(s) 121, 148, 1358
SchI GAGTC 5 cut(s) 7, 305, 488, 759, 965
ScrFI CCNGG 8 cut(s) 86, 178, 475, 579, 739, 884, 1008, 1362
SduI GDGCHC 5 cut(s) 66, 78, 333, 944, 1348
SfcI CTRYAG 1 cut(s) 534
SgrAI CRCCGGYG 1 cut(s) 215
SinI GGWCC 2 cut(s) 148, 1358
SmiMI CAYNNNNRTG 2 cut(s) 336, 1236
SpeI ACTAGT 1 cut(s) 1054
SphI GCATGC 1 cut(s) 851
Sse9I AATT 8 cut(s) 172, 426, 439, 719, 827, 1066, 1227, 1309
SseBI AGGCCT 1 cut(s) 617
SsiI CCGC 4 cut(s) 69, 166, 219, 1220
SspMI CTAG 3 cut(s) 1055, 1349, 1483
SstI GAGCTC 1 cut(s) 1348
StuI AGGCCT 1 cut(s) 617
StyD4I CCNGG 8 cut(s) 84, 176, 473, 577, 737, 882, 1006, 1360
StyI CCWWGG 1 cut(s) 873
TaaI ACNGT 4 cut(s) 61, 385, 653, 695
TaiI ACGT 1 cut(s) 246
TaqI TCGA 6 cut(s) 98, 300, 621, 702, 975, 1037
TasI AATT 8 cut(s) 172, 426, 439, 719, 827, 1066, 1227, 1309
TatI WGTACW 3 cut(s) 897, 978, 1302
TauI GCSGC 1 cut(s) 222
TfiI GAWTC 2 cut(s) 502, 908
Tru1I TTAA 2 cut(s) 947, 1428
Tru9I TTAA 2 cut(s) 947, 1428
TscAI CASTG 3 cut(s) 211, 658, 1305
TseFI GTSAC 1 cut(s) 751
TseI GCWGC 6 cut(s) 391, 533, 536, 734, 1014, 1222
Tsp45I GTSAC 1 cut(s) 751
TspDTI ATGAA 4 cut(s) 17, 626, 925, 1032
TspGWI ACGGA 1 cut(s) 380
TspRI CASTG 3 cut(s) 211, 658, 1305
Tth111I GACNNNGTC 1 cut(s) 242
Van91I CCANNNNNTGG 1 cut(s) 1442
VneI GTGCAC 2 cut(s) 62, 940
VpaK11BI GGWCC 2 cut(s) 148, 1358
VspI ATTAAT 2 cut(s) 947, 1428
XagI CCTNNNNNAGG 1 cut(s) 742
XapI RAATTY 2 cut(s) 827, 1227
XceI RCATGY 3 cut(s) 851, 898, 1031
XcmI CCANNNNNNNNNTGG 1 cut(s) 880
XmnI GAANNNNTTC 1 cut(s) 617
XspI CTAG 3 cut(s) 1055, 1349, 1483
Zsp2I ATGCAT 1 cut(s) 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.