RLG00000013983

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0004434
Physical Location & Seq
Reverse (-)
45102111 .. 45105630
3520 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013983

Sequence Viewer

Length: 1482 bp
ATGAAACCCATGACTCATTTCATCTCCCTCCTTCTCCTACTCTGCCTCCTCGCCACCGTCCACGGCGGAGGGCACGACTACCAAGACGCATTGTCGAAATGCATACTCTTTTTTGAGGGCCAGAGGTCGGGGGTTCTGCCTCAGGACCAACGCCAGTCGTGGCGGGCTAATTCCGGGTTGAGTGATGGCTGGACTTACAACACTGACCTCACCGGCGGCTACTACGACGCCGGAGACAACGTCAAGTTCGGGTTCCCGATGGCCTTCACGACCACAATGCTGGCGTGGAGTGTCATCGAGTTTGGGGACTCCATGCCTCCCAATGAGCACAGGAATGCGCTGGTTGCCCTTCGTTGGGGGACGGATTATCTCCTCAAAACGGTGTCGCAGCCCAATCGGATTTTCGTACAAGTCGGGGATCCAATTATAGACCACAATTGCTGGGAAAGACCAGAAGATATGGACACAGCCAGGATTGTATATGCCGTGGACTCGCCGAATCCAGCTTCTGATGTTGCCGGTGAGACAGCTGCGGCGCTGTCAGCTTCCTCAATGGCATTCCGATCCTCTGACCCTGGTTACGCAGACACATTGTTGCGAAATGCCATGAAGGCCTTCGAGTTTGCTAATACTTACAGAGGAGCTTACAGTGATAATGCCAATGTTAGAGATGGTGTCTGCCCATTTTACTGTGATTTCGATGGTTACCAAGACGAATTGCTATGGAGTGCAGCCTGGCTGAGGAGAGTCACTCAGGATGGTGGTTACCTTGACTACATACAAAACAATGGCAAAACTCTTGGTGCAGAGGACAATATAAACGAATTTGGTTGGGACAACAAGCATGCTGGCCTCAATGTTCTAATCTCCAAGGAAGTCCTGGAAGGCAACATGTACAACCTTGAATCATACAAATCATCGGCTGATAGCTTCATGTGCACATTAATACCGGAGTCGTCATCCTCCCACATCGAGTACACTCCAGCCGGTCTCATCTACAAGCCAGGAGGCAGCAATCTACAACATGTAACTTCGATTTCATTCCTACTACTAGTTTACGCCAATTACCTTGCACGAACCTCGCGATCCCTCAACTGTGGTAACATAGATGTCAGTCCAACAACGCTTCGCCAAATCGCCAAAAAGCAGGTTGATTATATTTTGGGTGATAATCCCATGGGTTTGTCATACATGGTTGGATACAGTGACCATTATCCGCAGCGAATTCACCACCGTGGATCTTCTTTACCATCTATCAAAGATCATCCCCAACCCATTGCTTGCAAGGAAGGCTCAGTGTACTTCAATTCGTCAAACCCTAACCCTAATCCCCTTGTGGGAGCCCTAGTAGGAGGACCTGGAGAAGATGATGTCTATGAGGATGATCGCGCTGATTTTAGGAAGTCCGAACCGACCACGTACATTAATGCACCATTTGTTGGTGTTTTGGCCTACTTTGTGGCTAATCCCAGTCAAAACTAG

Protein Analysis

494

Amino Acids

54.52

Weight (kDa)

4.93

Isoelectric Point (pI)

46.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 27 - 483 4.2e-156 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1138
AccB7I CCANNNNNTGG 1 cut(s) 1439
AccII CGCG 2 cut(s) 1084, 1389
AciI CCGC 5 cut(s) 66, 163, 216, 533, 1217
AclWI GGATC 5 cut(s) 413, 426, 558, 1080, 1246
AcsI RAATTY 2 cut(s) 824, 1224
AcyI GRCGYC 1 cut(s) 228
AfaI GTAC 5 cut(s) 408, 896, 977, 1301, 1421
AfiI CCNNNNNNNGG 7 cut(s) 127, 354, 355, 379, 741, 1337, 1439
AflIII ACRYGT 2 cut(s) 891, 1024
AgsI TTSAA 2 cut(s) 905, 1306
AhdI GACNNNNNGTC 1 cut(s) 91
AhlI ACTAGT 1 cut(s) 1051
AjnI CCWGG 6 cut(s) 470, 574, 734, 879, 1003, 1357
AjuI GAANNNNNNNTTGG 2 cut(s) 386, 418
AleI CACNNNNGTG 1 cut(s) 1233
AluBI AGCT 5 cut(s) 506, 530, 545, 644, 930
AluI AGCT 5 cut(s) 506, 530, 545, 644, 930
Alw21I GWGCWC 2 cut(s) 330, 941
Alw26I GTCTC 3 cut(s) 228, 518, 995
Alw44I GTGCAC 1 cut(s) 937
AlwI GGATC 5 cut(s) 413, 426, 558, 1080, 1246
AlwNI CAGNNNCTG 1 cut(s) 509
AoxI GGCC 5 cut(s) 118, 261, 612, 850, 1449
ApaLI GTGCAC 1 cut(s) 937
ApeKI GCWGC 5 cut(s) 388, 530, 731, 1011, 1219
ApoI RAATTY 2 cut(s) 824, 1224
AseI ATTAAT 2 cut(s) 944, 1425
Asp700I GAANNNNTTC 1 cut(s) 614
AspLEI GCGC 3 cut(s) 340, 538, 1391
AspS9I GGNCC 3 cut(s) 118, 145, 1355
AsuC2I CCSGG 1 cut(s) 175
AsuHPI GGTGA 4 cut(s) 202, 533, 1178, 1220
AvaII GGWCC 2 cut(s) 145, 1355
AxyI CCTNAGG 1 cut(s) 141
BaeGI GKGCMC 2 cut(s) 75, 941
BamHI GGATCC 1 cut(s) 418
BanII GRGCYC 1 cut(s) 1345
Bbv12I GWGCWC 2 cut(s) 330, 941
BbvCI CCTCAGC 1 cut(s) 740
BbvI GCAGC 5 cut(s) 400, 517, 743, 1023, 1231
BccI CCATC 6 cut(s) 179, 253, 665, 695, 752, 1258
BceAI ACGGC 2 cut(s) 79, 470
BcgI CGANNNNNNTGC 6 cut(s) 259, 293, 377, 411, 1062, 1096
BciT130I CCWGG 6 cut(s) 472, 576, 736, 881, 1005, 1359
BciVI GTATCC 1 cut(s) 1193
BcnI CCSGG 1 cut(s) 175
BcoDI GTCTC 3 cut(s) 228, 518, 995
BcuI ACTAGT 1 cut(s) 1051
BfaI CTAG 3 cut(s) 1052, 1346, 1480
BfoI RGCGCY 1 cut(s) 539
BfuAI ACCTGC 1 cut(s) 1138
BfuI GTATCC 1 cut(s) 1193
BglI GCCNNNNNGGC 1 cut(s) 611
BisI GCNGC 7 cut(s) 217, 389, 531, 534, 732, 1012, 1220
BlsI GCNGC 7 cut(s) 218, 390, 532, 535, 733, 1013, 1221
Bme1390I CCNGG 7 cut(s) 175, 472, 576, 736, 881, 1005, 1359
Bme18I GGWCC 2 cut(s) 145, 1355
BmeRI GACNNNNNGTC 1 cut(s) 91
BmgT120I GGNCC 3 cut(s) 118, 145, 1355
BmiI GGNNCC 3 cut(s) 254, 420, 1342
BmrFI CCNGG 7 cut(s) 175, 472, 576, 736, 881, 1005, 1359
BmrI ACTGGG 1 cut(s) 1464
BmuI ACTGGG 1 cut(s) 1464
BplI GAGNNNNNCTC 2 cut(s) 736, 768
BpmI CTGGAG 2 cut(s) 966, 1380
Bpu10I CCTNAGC 1 cut(s) 740
BpuMI CCSGG 1 cut(s) 175
BsaAI YACGTR 1 cut(s) 1419
BsaHI GRCGYC 1 cut(s) 228
BsaI GGTCTC 1 cut(s) 995
BsaJI CCNNGG 6 cut(s) 61, 486, 574, 870, 1176, 1234
BsaWI WCCGGW 1 cut(s) 949
BsaXI ACNNNNNCTCC 6 cut(s) 30, 60, 225, 255, 1332, 1362
Bsc4I CCNNNNNNNGG 7 cut(s) 127, 354, 355, 379, 741, 1337, 1439
Bse118I RCCGGY 3 cut(s) 212, 518, 986
Bse1I ACTGG 2 cut(s) 154, 1470
Bse21I CCTNAGG 1 cut(s) 141
Bse3DI GCAATG 1 cut(s) 1275
BseBI CCWGG 6 cut(s) 472, 576, 736, 881, 1005, 1359
BseDI CCNNGG 6 cut(s) 61, 486, 574, 870, 1176, 1234
BseGI GGATG 4 cut(s) 763, 959, 1264, 1387
BseLI CCNNNNNNNGG 7 cut(s) 127, 354, 355, 379, 741, 1337, 1439
BseMI GCAATG 1 cut(s) 1275
BseMII CTCAG 4 cut(s) 155, 731, 767, 1308
BseNI ACTGG 2 cut(s) 154, 1470
BseRI GAGGAG 4 cut(s) 38, 362, 654, 757
BseSI GKGCMC 2 cut(s) 75, 941
BseXI GCAGC 5 cut(s) 400, 517, 743, 1023, 1231
BseYI CCCAGC 1 cut(s) 441
BsgI GTGCAG 2 cut(s) 750, 825
Bsh1236I CGCG 2 cut(s) 1084, 1389
BshFI GGCC 5 cut(s) 120, 263, 614, 852, 1451
BsiHKAI GWGCWC 2 cut(s) 330, 941
BsiSI CCGG 6 cut(s) 174, 213, 231, 519, 950, 987
BslFI GGGAC 3 cut(s) 320, 373, 848
BslI CCNNNNNNNGG 7 cut(s) 127, 354, 355, 379, 741, 1337, 1439
BsmAI GTCTC 3 cut(s) 228, 518, 995
BsmFI GGGAC 3 cut(s) 320, 373, 848
BsmI GAATGC 2 cut(s) 340, 557
BsnI GGCC 5 cut(s) 120, 263, 614, 852, 1451
Bso31I GGTCTC 1 cut(s) 995
Bsp1286I GDGCHC 4 cut(s) 75, 330, 941, 1345
Bsp1407I TGTACA 1 cut(s) 894
Bsp143I GATC 6 cut(s) 418, 563, 1085, 1238, 1261, 1384
Bsp19I CCATGG 1 cut(s) 1176
Bsp68I TCGCGA 1 cut(s) 1084
BspACI CCGC 5 cut(s) 66, 163, 216, 533, 1217
BspANI GGCC 5 cut(s) 120, 263, 614, 852, 1451
BspCNI CTCAG 4 cut(s) 154, 732, 766, 1307
BspFNI CGCG 2 cut(s) 1084, 1389
BspLI GGNNCC 3 cut(s) 254, 420, 1342
BspMI ACCTGC 1 cut(s) 1138
BspPI GGATC 5 cut(s) 413, 426, 558, 1080, 1246
BspTNI GGTCTC 1 cut(s) 995
BsrDI GCAATG 1 cut(s) 1275
BsrFI RCCGGY 3 cut(s) 212, 518, 986
BsrGI TGTACA 1 cut(s) 894
BsrI ACTGG 2 cut(s) 154, 1470
BssAI RCCGGY 3 cut(s) 212, 518, 986
BssECI CCNNGG 6 cut(s) 61, 486, 574, 870, 1176, 1234
BssMI GATC 6 cut(s) 418, 563, 1085, 1238, 1261, 1384
BssNI GRCGYC 1 cut(s) 228
BssT1I CCWWGG 2 cut(s) 870, 1176
Bst2UI CCWGG 6 cut(s) 472, 576, 736, 881, 1005, 1359
Bst4CI ACNGT 7 cut(s) 58, 382, 650, 692, 1097, 1205, 1235
BstACI GRCGYC 1 cut(s) 228
BstAUI TGTACA 1 cut(s) 894
BstBAI YACGTR 1 cut(s) 1419
BstC8I GCNNGC 5 cut(s) 165, 282, 846, 850, 1282
BstDEI CTNAG 4 cut(s) 141, 740, 753, 1294
BstDSI CCRYGG 4 cut(s) 61, 486, 1176, 1234
BstEII GGTNACC 2 cut(s) 704, 764
BstENI CCTNNNNNAGG 1 cut(s) 739
BstF5I GGATG 4 cut(s) 763, 959, 1264, 1387
BstFNI CGCG 2 cut(s) 1084, 1389
BstH2I RGCGCY 1 cut(s) 539
BstHHI GCGC 3 cut(s) 340, 538, 1391
BstKTI GATC 6 cut(s) 421, 566, 1088, 1241, 1264, 1387
BstMAI GTCTC 3 cut(s) 228, 518, 995
BstMBI GATC 6 cut(s) 418, 563, 1085, 1238, 1261, 1384
BstMWI GCNNNNNNNGC 5 cut(s) 344, 542, 611, 936, 1290
BstNI CCWGG 6 cut(s) 472, 576, 736, 881, 1005, 1359
BstNSI RCATGY 3 cut(s) 848, 895, 1028
BstPI GGTNACC 2 cut(s) 704, 764
BstSCI CCNGG 7 cut(s) 173, 470, 574, 734, 879, 1003, 1357
BstSLI GKGCMC 2 cut(s) 75, 941
BstUI CGCG 2 cut(s) 1084, 1389
BstV1I GCAGC 5 cut(s) 400, 517, 743, 1023, 1231
BstX2I RGATCY 2 cut(s) 418, 1238
BstXI CCANNNNNNTGG 1 cut(s) 280
BstYI RGATCY 2 cut(s) 418, 1238
Bsu36I CCTNAGG 1 cut(s) 141
BsuI GTATCC 1 cut(s) 1193
BsuRI GGCC 5 cut(s) 120, 263, 614, 852, 1451
BtgI CCRYGG 4 cut(s) 61, 486, 1176, 1234
BtsCI GGATG 4 cut(s) 763, 959, 1264, 1387
BtsIMutI CAGTG 4 cut(s) 201, 655, 1210, 1302
BtuMI TCGCGA 1 cut(s) 1084
BveI ACCTGC 1 cut(s) 1138
Cac8I GCNNGC 5 cut(s) 165, 282, 846, 850, 1282
CaiI CAGNNNCTG 1 cut(s) 509
CfoI GCGC 3 cut(s) 340, 538, 1391
Cfr10I RCCGGY 3 cut(s) 212, 518, 986
Cfr13I GGNCC 3 cut(s) 118, 145, 1355
CseI GACGC 2 cut(s) 95, 236
Csp6I GTAC 5 cut(s) 407, 895, 976, 1300, 1420
CviAII CATG 9 cut(s) 10, 313, 607, 845, 892, 934, 1025, 1177, 1192
CviQI GTAC 5 cut(s) 407, 895, 976, 1300, 1420
DdeI CTNAG 4 cut(s) 141, 740, 753, 1294
DpnI GATC 6 cut(s) 420, 565, 1087, 1240, 1263, 1386
DpnII GATC 6 cut(s) 418, 563, 1085, 1238, 1261, 1384
DriI GACNNNNNGTC 1 cut(s) 91
Eam1105I GACNNNNNGTC 1 cut(s) 91
EciI GGCGGA 1 cut(s) 81
Eco130I CCWWGG 2 cut(s) 870, 1176
Eco147I AGGCCT 1 cut(s) 614
Eco24I GRGCYC 1 cut(s) 1345
Eco31I GGTCTC 1 cut(s) 995
Eco47I GGWCC 2 cut(s) 145, 1355
Eco81I CCTNAGG 1 cut(s) 141
Eco91I GGTNACC 2 cut(s) 704, 764
EcoNI CCTNNNNNAGG 1 cut(s) 739
EcoO109I RGGNCCY 1 cut(s) 1355
EcoO65I GGTNACC 2 cut(s) 704, 764
EcoRI GAATTC 1 cut(s) 1224
EcoRII CCWGG 6 cut(s) 470, 574, 734, 879, 1003, 1357
EcoT14I CCWWGG 2 cut(s) 870, 1176
EcoT22I ATGCAT 1 cut(s) 104
EcoT38I GRGCYC 1 cut(s) 1345
ErhI CCWWGG 2 cut(s) 870, 1176
FaeI CATG 9 cut(s) 13, 316, 610, 848, 895, 937, 1028, 1180, 1195
FaqI GGGAC 3 cut(s) 320, 373, 848
FatI CATG 9 cut(s) 9, 312, 606, 844, 891, 933, 1024, 1176, 1191
FauI CCCGC 1 cut(s) 156
Fnu4HI GCNGC 7 cut(s) 217, 389, 531, 534, 732, 1012, 1220
FokI GGATG 4 cut(s) 770, 946, 1251, 1394
FriOI GRGCYC 1 cut(s) 1345
Fsp4HI GCNGC 7 cut(s) 217, 389, 531, 534, 732, 1012, 1220
FspBI CTAG 3 cut(s) 1052, 1346, 1480
GlaI GCGC 3 cut(s) 339, 537, 1390
GluI GCNGC 7 cut(s) 217, 389, 531, 534, 732, 1012, 1220
GsaI CCCAGC 1 cut(s) 445
GsuI CTGGAG 2 cut(s) 966, 1380
HaeII RGCGCY 1 cut(s) 539
HaeIII GGCC 5 cut(s) 120, 263, 614, 852, 1451
HapII CCGG 6 cut(s) 174, 213, 231, 519, 950, 987
HgaI GACGC 2 cut(s) 95, 236
HhaI GCGC 3 cut(s) 340, 538, 1391
Hin1I GRCGYC 1 cut(s) 228
Hin1II CATG 9 cut(s) 13, 316, 610, 848, 895, 937, 1028, 1180, 1195
Hin6I GCGC 3 cut(s) 338, 536, 1389
HinP1I GCGC 3 cut(s) 338, 536, 1389
HinfI GANTC 7 cut(s) 13, 308, 491, 499, 747, 905, 953
HpaII CCGG 6 cut(s) 174, 213, 231, 519, 950, 987
HphI GGTGA 4 cut(s) 202, 533, 1178, 1220
Hpy166II GTNNAC 6 cut(s) 61, 490, 939, 978, 1057, 1300
Hpy188I TCNGA 5 cut(s) 399, 511, 563, 571, 1408
Hpy188III TCNNGA 5 cut(s) 143, 256, 268, 755, 1083
Hpy8I GTNNAC 6 cut(s) 61, 490, 939, 978, 1057, 1300
Hpy99I CGWCG 1 cut(s) 230
HpyAV CCTTC 7 cut(s) 41, 274, 359, 604, 625, 878, 1283
HpyCH4III ACNGT 7 cut(s) 58, 382, 650, 692, 1097, 1205, 1235
HpyCH4IV ACGT 2 cut(s) 240, 1418
HpyCH4V TGCA 7 cut(s) 102, 731, 806, 939, 1073, 1284, 1430
HpyF10VI GCNNNNNNNGC 5 cut(s) 344, 542, 611, 936, 1290
HpyF3I CTNAG 4 cut(s) 141, 740, 753, 1294
HpySE526I ACGT 2 cut(s) 240, 1418
Hsp92I GRCGYC 1 cut(s) 228
Hsp92II CATG 9 cut(s) 13, 316, 610, 848, 895, 937, 1028, 1180, 1195
HspAI GCGC 3 cut(s) 338, 536, 1389
Kzo9I GATC 6 cut(s) 418, 563, 1085, 1238, 1261, 1384
LmnI GCTCC 2 cut(s) 641, 1340
Lsp1109I GCAGC 5 cut(s) 400, 517, 743, 1023, 1231
MaeI CTAG 3 cut(s) 1052, 1346, 1480
MaeII ACGT 2 cut(s) 240, 1418
MaeIII GTNAC 7 cut(s) 578, 704, 748, 764, 1027, 1100, 1205
MalI GATC 6 cut(s) 420, 565, 1087, 1240, 1263, 1386
MboI GATC 6 cut(s) 418, 563, 1085, 1238, 1261, 1384
MboII GAAGA 3 cut(s) 467, 1233, 1376
MfeI CAATTG 1 cut(s) 436
MflI RGATCY 2 cut(s) 418, 1238
MhlI GDGCHC 4 cut(s) 75, 330, 941, 1345
MluCI AATT 8 cut(s) 169, 423, 436, 716, 824, 1063, 1224, 1306
MlyI GAGTC 5 cut(s) 7, 302, 485, 756, 962
MmeI TCCRAC 2 cut(s) 1142, 1177
Mph1103I ATGCAT 1 cut(s) 104
MroXI GAANNNNTTC 1 cut(s) 614
MseI TTAA 2 cut(s) 944, 1425
MslI CAYNNNNRTG 2 cut(s) 333, 1233
MspA1I CMGCKG 1 cut(s) 530
MspI CCGG 6 cut(s) 174, 213, 231, 519, 950, 987
MspR9I CCNGG 7 cut(s) 175, 472, 576, 736, 881, 1005, 1359
MunI CAATTG 1 cut(s) 436
Mva1269I GAATGC 2 cut(s) 340, 557
MvaI CCWGG 6 cut(s) 472, 576, 736, 881, 1005, 1359
MvnI CGCG 2 cut(s) 1084, 1389
MwoI GCNNNNNNNGC 5 cut(s) 344, 542, 611, 936, 1290
NciI CCSGG 1 cut(s) 175
NcoI CCATGG 1 cut(s) 1176
NdeII GATC 6 cut(s) 418, 563, 1085, 1238, 1261, 1384
NlaIII CATG 9 cut(s) 13, 316, 610, 848, 895, 937, 1028, 1180, 1195
NlaIV GGNNCC 3 cut(s) 254, 420, 1342
NmuCI GTSAC 2 cut(s) 748, 1205
NruI TCGCGA 1 cut(s) 1084
NsiI ATGCAT 1 cut(s) 104
NspI RCATGY 3 cut(s) 848, 895, 1028
OliI CACNNNNGTG 1 cut(s) 1233
PaeI GCATGC 1 cut(s) 848
PceI AGGCCT 1 cut(s) 614
PciI ACATGT 2 cut(s) 891, 1024
PcsI WCGNNNNNNNCGW 1 cut(s) 1081
PctI GAATGC 2 cut(s) 340, 557
PdmI GAANNNNTTC 1 cut(s) 614
PfeI GAWTC 2 cut(s) 499, 905
PflFI GACNNNGTC 1 cut(s) 239
PflMI CCANNNNNTGG 1 cut(s) 1439
PfoI TCCNGGA 1 cut(s) 879
PkrI GCNGC 7 cut(s) 218, 390, 532, 535, 733, 1013, 1221
PleI GAGTC 5 cut(s) 7, 302, 485, 755, 961
PpsI GAGTC 5 cut(s) 7, 302, 485, 755, 961
Ppu21I YACGTR 1 cut(s) 1419
PpuMI RGGWCCY 1 cut(s) 1355
PscI ACATGT 2 cut(s) 891, 1024
PshBI ATTAAT 2 cut(s) 944, 1425
Psp5II RGGWCCY 1 cut(s) 1355
Psp6I CCWGG 6 cut(s) 470, 574, 734, 879, 1003, 1357
PspEI GGTNACC 2 cut(s) 704, 764
PspFI CCCAGC 1 cut(s) 441
PspGI CCWGG 6 cut(s) 470, 574, 734, 879, 1003, 1357
PspN4I GGNNCC 3 cut(s) 254, 420, 1342
PspPI GGNCC 3 cut(s) 118, 145, 1355
PspPPI RGGWCCY 1 cut(s) 1355
PstNI CAGNNNCTG 1 cut(s) 509
PsuI RGATCY 2 cut(s) 418, 1238
PsyI GACNNNGTC 1 cut(s) 239
PvuII CAGCTG 1 cut(s) 530
RruI TCGCGA 1 cut(s) 1084
RsaI GTAC 5 cut(s) 408, 896, 977, 1301, 1421
RsaNI GTAC 5 cut(s) 407, 895, 976, 1300, 1420
RseI CAYNNNNRTG 2 cut(s) 333, 1233
SaqAI TTAA 2 cut(s) 944, 1425
SatI GCNGC 7 cut(s) 217, 389, 531, 534, 732, 1012, 1220
Sau3AI GATC 6 cut(s) 418, 563, 1085, 1238, 1261, 1384
Sau96I GGNCC 3 cut(s) 118, 145, 1355
SchI GAGTC 5 cut(s) 7, 302, 485, 756, 962
ScrFI CCNGG 7 cut(s) 175, 472, 576, 736, 881, 1005, 1359
SduI GDGCHC 4 cut(s) 75, 330, 941, 1345
SgrAI CRCCGGYG 1 cut(s) 212
SinI GGWCC 2 cut(s) 145, 1355
SmiMI CAYNNNNRTG 2 cut(s) 333, 1233
SpeI ACTAGT 1 cut(s) 1051
SphI GCATGC 1 cut(s) 848
Sse9I AATT 8 cut(s) 169, 423, 436, 716, 824, 1063, 1224, 1306
SseBI AGGCCT 1 cut(s) 614
SsiI CCGC 5 cut(s) 66, 163, 216, 533, 1217
SspMI CTAG 3 cut(s) 1052, 1346, 1480
StuI AGGCCT 1 cut(s) 614
StyD4I CCNGG 7 cut(s) 173, 470, 574, 734, 879, 1003, 1357
StyI CCWWGG 2 cut(s) 870, 1176
TaaI ACNGT 7 cut(s) 58, 382, 650, 692, 1097, 1205, 1235
TaiI ACGT 2 cut(s) 243, 1421
TaqI TCGA 6 cut(s) 95, 297, 618, 699, 972, 1034
TasI AATT 8 cut(s) 169, 423, 436, 716, 824, 1063, 1224, 1306
TatI WGTACW 3 cut(s) 894, 975, 1299
TauI GCSGC 2 cut(s) 219, 536
TfiI GAWTC 2 cut(s) 499, 905
Tru1I TTAA 2 cut(s) 944, 1425
Tru9I TTAA 2 cut(s) 944, 1425
TscAI CASTG 4 cut(s) 208, 655, 1210, 1302
TseFI GTSAC 2 cut(s) 748, 1205
TseI GCWGC 5 cut(s) 388, 530, 731, 1011, 1219
Tsp45I GTSAC 2 cut(s) 748, 1205
TspDTI ATGAA 5 cut(s) 10, 17, 623, 922, 1029
TspGWI ACGGA 1 cut(s) 377
TspRI CASTG 4 cut(s) 208, 655, 1210, 1302
Tth111I GACNNNGTC 1 cut(s) 239
Van91I CCANNNNNTGG 1 cut(s) 1439
VneI GTGCAC 1 cut(s) 937
VpaK11BI GGWCC 2 cut(s) 145, 1355
VspI ATTAAT 2 cut(s) 944, 1425
XagI CCTNNNNNAGG 1 cut(s) 739
XapI RAATTY 2 cut(s) 824, 1224
XceI RCATGY 3 cut(s) 848, 895, 1028
XcmI CCANNNNNNNNNTGG 1 cut(s) 877
XmnI GAANNNNTTC 1 cut(s) 614
XspI CTAG 3 cut(s) 1052, 1346, 1480
Zsp2I ATGCAT 1 cut(s) 104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.