Rh6AG156000

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0004434
Physical Location & Seq
Forward (+)
24303414 .. 24303719
306 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG156000.1

Sequence Viewer

Length: 306 bp
ATGGGTTTGTCATACATGGCCGGATACAGCTACCATTATCCACAGCGAATTCACCACCGTGGATCTTCTTTACCTTCTATCAAGGATCATCCCCAACCCATTGCTTGCAAGGAAGGCTCAGTGTACTTCAATTCGTCAAACCCTAACCCTAATCCCCTTGTGGGAGCCCTAGTAGGAGGATCTAGAGAAGATGATGTCTATGAGGATGATTGCGCTGATTTTAGGAAGTCTGAACCGACCACATACATTAATGCACCATTTGTTGGTGTTTTGGCATACTTTGTGGCTAATCCCAGTCCAAACTAG

Protein Analysis

101

Amino Acids

11.1

Weight (kDa)

5.67

Isoelectric Point (pI)

50.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 1 - 91 2.2e-24 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 263
AclWI GGATC 3 cut(s) 70, 93, 187
AcoI YGGCCR 1 cut(s) 18
AcsI RAATTY 1 cut(s) 48
AfaI GTAC 1 cut(s) 125
AfiI CCNNNNNNNGG 2 cut(s) 161, 263
AgsI TTSAA 1 cut(s) 130
AleI CACNNNNGTG 1 cut(s) 57
AluBI AGCT 1 cut(s) 30
AluI AGCT 1 cut(s) 30
AlwI GGATC 3 cut(s) 70, 93, 187
AoxI GGCC 1 cut(s) 18
ApoI RAATTY 1 cut(s) 48
AseI ATTAAT 1 cut(s) 249
AspLEI GCGC 1 cut(s) 215
AsuHPI GGTGA 1 cut(s) 44
BanII GRGCYC 1 cut(s) 169
BciVI GTATCC 1 cut(s) 17
BfaI CTAG 3 cut(s) 170, 183, 304
BfuI GTATCC 1 cut(s) 17
BmiI GGNNCC 1 cut(s) 166
BmrI ACTGGG 1 cut(s) 288
BmuI ACTGGG 1 cut(s) 288
BsaJI CCNNGG 1 cut(s) 58
BsaXI ACNNNNNCTCC 2 cut(s) 156, 186
Bsc4I CCNNNNNNNGG 2 cut(s) 161, 263
Bse1I ACTGG 1 cut(s) 294
Bse3DI GCAATG 1 cut(s) 99
BseDI CCNNGG 1 cut(s) 58
BseGI GGATG 2 cut(s) 88, 211
BseLI CCNNNNNNNGG 2 cut(s) 161, 263
BseMI GCAATG 1 cut(s) 99
BseMII CTCAG 1 cut(s) 132
BseNI ACTGG 1 cut(s) 294
BshFI GGCC 1 cut(s) 20
BsiSI CCGG 1 cut(s) 21
BslI CCNNNNNNNGG 2 cut(s) 161, 263
BsnI GGCC 1 cut(s) 20
Bsp1286I GDGCHC 1 cut(s) 169
Bsp143I GATC 3 cut(s) 62, 85, 179
BspANI GGCC 1 cut(s) 20
BspCNI CTCAG 1 cut(s) 131
BspLI GGNNCC 1 cut(s) 166
BspPI GGATC 3 cut(s) 70, 93, 187
BsrDI GCAATG 1 cut(s) 99
BsrI ACTGG 1 cut(s) 294
BssECI CCNNGG 1 cut(s) 58
BssMI GATC 3 cut(s) 62, 85, 179
Bst4CI ACNGT 1 cut(s) 59
BstC8I GCNNGC 1 cut(s) 106
BstDEI CTNAG 1 cut(s) 118
BstDSI CCRYGG 1 cut(s) 58
BstF5I GGATG 2 cut(s) 88, 211
BstHHI GCGC 1 cut(s) 215
BstKTI GATC 3 cut(s) 65, 88, 182
BstMBI GATC 3 cut(s) 62, 85, 179
BstMWI GCNNNNNNNGC 1 cut(s) 114
BstX2I RGATCY 2 cut(s) 62, 179
BstYI RGATCY 2 cut(s) 62, 179
BsuI GTATCC 1 cut(s) 17
BsuRI GGCC 1 cut(s) 20
BtgI CCRYGG 1 cut(s) 58
BtsCI GGATG 2 cut(s) 88, 211
BtsIMutI CAGTG 1 cut(s) 126
Cac8I GCNNGC 1 cut(s) 106
CfoI GCGC 1 cut(s) 215
Csp6I GTAC 1 cut(s) 124
CviAII CATG 1 cut(s) 16
CviJI RGCY 5 cut(s) 20, 30, 117, 167, 287
CviKI_1 RGCY 5 cut(s) 20, 30, 117, 167, 287
CviQI GTAC 1 cut(s) 124
DdeI CTNAG 1 cut(s) 118
DpnI GATC 3 cut(s) 64, 87, 181
DpnII GATC 3 cut(s) 62, 85, 179
EaeI YGGCCR 1 cut(s) 18
Eco24I GRGCYC 1 cut(s) 169
EcoRI GAATTC 1 cut(s) 48
EcoT38I GRGCYC 1 cut(s) 169
FaeI CATG 1 cut(s) 19
FaiI YATR 5 cut(s) 13, 17, 201, 244, 277
FatI CATG 1 cut(s) 15
FokI GGATG 2 cut(s) 75, 218
FriOI GRGCYC 1 cut(s) 169
FspBI CTAG 3 cut(s) 170, 183, 304
GlaI GCGC 1 cut(s) 214
HaeIII GGCC 1 cut(s) 20
HapII CCGG 1 cut(s) 21
HhaI GCGC 1 cut(s) 215
Hin1II CATG 1 cut(s) 19
Hin6I GCGC 1 cut(s) 213
HinP1I GCGC 1 cut(s) 213
HpaII CCGG 1 cut(s) 21
HphI GGTGA 1 cut(s) 44
Hpy166II GTNNAC 1 cut(s) 124
Hpy188I TCNGA 1 cut(s) 232
Hpy188III TCNNGA 1 cut(s) 183
Hpy8I GTNNAC 1 cut(s) 124
HpyAV CCTTC 2 cut(s) 84, 107
HpyCH4III ACNGT 1 cut(s) 59
HpyCH4V TGCA 2 cut(s) 108, 254
HpyF10VI GCNNNNNNNGC 1 cut(s) 114
HpyF3I CTNAG 1 cut(s) 118
Hsp92II CATG 1 cut(s) 19
HspAI GCGC 1 cut(s) 213
Kzo9I GATC 3 cut(s) 62, 85, 179
LmnI GCTCC 1 cut(s) 164
LpnPI CCDG 1 cut(s) 34
MaeI CTAG 3 cut(s) 170, 183, 304
MalI GATC 3 cut(s) 64, 87, 181
MboI GATC 3 cut(s) 62, 85, 179
MboII GAAGA 2 cut(s) 57, 200
MflI RGATCY 2 cut(s) 62, 179
MhlI GDGCHC 1 cut(s) 169
MluCI AATT 2 cut(s) 48, 130
MnlI CCTC 2 cut(s) 170, 196
MseI TTAA 1 cut(s) 249
MslI CAYNNNNRTG 1 cut(s) 57
MspI CCGG 1 cut(s) 21
MwoI GCNNNNNNNGC 1 cut(s) 114
NdeII GATC 3 cut(s) 62, 85, 179
NlaIII CATG 1 cut(s) 19
NlaIV GGNNCC 1 cut(s) 166
OliI CACNNNNGTG 1 cut(s) 57
PflMI CCANNNNNTGG 1 cut(s) 263
PshBI ATTAAT 1 cut(s) 249
PspN4I GGNNCC 1 cut(s) 166
PsuI RGATCY 2 cut(s) 62, 179
RsaI GTAC 1 cut(s) 125
RsaNI GTAC 1 cut(s) 124
RseI CAYNNNNRTG 1 cut(s) 57
SaqAI TTAA 1 cut(s) 249
Sau3AI GATC 3 cut(s) 62, 85, 179
SduI GDGCHC 1 cut(s) 169
SetI ASST 2 cut(s) 32, 76
SgeI CNNG 9 cut(s) 28, 33, 71, 94, 117, 121, 170, 182, 195
SmiMI CAYNNNNRTG 1 cut(s) 57
Sse9I AATT 2 cut(s) 48, 130
SspMI CTAG 3 cut(s) 170, 183, 304
TaaI ACNGT 1 cut(s) 59
TasI AATT 2 cut(s) 48, 130
TatI WGTACW 1 cut(s) 123
Tru1I TTAA 1 cut(s) 249
Tru9I TTAA 1 cut(s) 249
TscAI CASTG 1 cut(s) 126
TspRI CASTG 1 cut(s) 126
Van91I CCANNNNNTGG 1 cut(s) 263
VspI ATTAAT 1 cut(s) 249
XapI RAATTY 1 cut(s) 48
XbaI TCTAGA 1 cut(s) 182
XspI CTAG 3 cut(s) 170, 183, 304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.