Rw6G013530

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0004434
Physical Location & Seq
Forward (+)
25063691 .. 25065003
1313 bp
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UTR
Exon/CDS
Intron
Rw6G013530.1

Sequence Viewer

Length: 816 bp
ATGGACACAGCCAGGACTATATATGCCGTGGACTCGCCGAATCCAGCTTCTGATGTTGCCGGTGAGACAGCTGCGGCACTGTCAGTTTCCTCAATGGCATTCCGATCCGCTAACCTAGGTTACGCAGACACATTGTTGCGAAATGCCATGAAGGCCTTCGAGTTTGCTGATACTTACAGAGGAGCTTACAGTGATAATGCCAATGTTAGAGATGGTGTCTGCCCATTTTACTGTGATTTCGATGGTTACCAAGACGAATTGCTATGGAGTGCAGCCAGGCTGAGGAGAGCCACTCAGGATGGTGGTTACCTTGACAACATACAAAACAATGGCAAAACTCTTGGTGCAGAGGACAATATAAACGAATTCGGTTGGGACAACAAGCATGCTGGCCTCAATGAAGTCCTGGAAGGCAACATGTACAACCTTGAATCCTACAAATCATCGGCTGATAGCTTCATGTGCACATGCATTAATACCGGAATCATCATCCTCCACATCGAGTACACTCCAGCCGGTCTCATCTACAAGCCAGGAGGCAGCAATCTGCAACATGCAACTTCGATTTCATTCCTACTACTAGTTTACGCCAATTACCTTGCACGAACCTCGCGATCCCTCAACTGTGGAAACATAGATGTCAGTCCAAAAACGCTTCGCCAAATAGCCAATGGGTTTGTCATACATGGTCGGATACAGCGACCATTATCCACAGCGAATTCACCACCGTGGATCTTCTTTACCTTCTATCAAGGATCATCCCCAAACCATTGCTTGCAAGGAAGGCTCAGTGTACTTCAATTCGTCAAACCCTAA

Protein Analysis

271

Amino Acids

29.81

Weight (kDa)

5.71

Isoelectric Point (pI)

50.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 1 - 223 2.4e-46 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 613
AciI CCGC 2 cut(s) 74, 108
AclWI GGATC 4 cut(s) 99, 609, 740, 763
AcsI RAATTY 2 cut(s) 365, 718
AfaI GTAC 3 cut(s) 422, 506, 795
AfiI CCNNNNNNNGG 1 cut(s) 282
AflIII ACRYGT 1 cut(s) 417
AgsI TTSAA 2 cut(s) 431, 800
AhlI ACTAGT 1 cut(s) 580
AjnI CCWGG 4 cut(s) 11, 275, 405, 532
AleI CACNNNNGTG 1 cut(s) 727
AluBI AGCT 4 cut(s) 47, 71, 185, 456
AluI AGCT 4 cut(s) 47, 71, 185, 456
Alw21I GWGCWC 1 cut(s) 467
Alw26I GTCTC 2 cut(s) 59, 524
Alw44I GTGCAC 1 cut(s) 463
AlwI GGATC 4 cut(s) 99, 609, 740, 763
AlwNI CAGNNNCTG 1 cut(s) 50
AoxI GGCC 2 cut(s) 153, 391
ApaLI GTGCAC 1 cut(s) 463
ApeKI GCWGC 3 cut(s) 71, 272, 540
ApoI RAATTY 2 cut(s) 365, 718
AseI ATTAAT 1 cut(s) 474
Asp700I GAANNNNTTC 1 cut(s) 155
AspA2I CCTAGG 1 cut(s) 115
AsuHPI GGTGA 2 cut(s) 74, 714
AvrII CCTAGG 1 cut(s) 115
BaeGI GKGCMC 1 cut(s) 467
Bbv12I GWGCWC 1 cut(s) 467
BbvCI CCTCAGC 1 cut(s) 281
BbvI GCAGC 3 cut(s) 58, 284, 552
BccI CCATC 3 cut(s) 206, 236, 293
BceAI ACGGC 1 cut(s) 11
BcgI CGANNNNNNTGC 2 cut(s) 591, 625
BciT130I CCWGG 4 cut(s) 13, 277, 407, 534
BciVI GTATCC 1 cut(s) 687
BcoDI GTCTC 2 cut(s) 59, 524
BcuI ACTAGT 1 cut(s) 580
BfaI CTAG 2 cut(s) 116, 581
BfuI GTATCC 1 cut(s) 687
BglI GCCNNNNNGGC 1 cut(s) 152
BisI GCNGC 4 cut(s) 72, 75, 273, 541
BlnI CCTAGG 1 cut(s) 115
BlsI GCNGC 4 cut(s) 73, 76, 274, 542
Bme1390I CCNGG 4 cut(s) 13, 277, 407, 534
BmrFI CCNGG 4 cut(s) 13, 277, 407, 534
BplI GAGNNNNNCTC 2 cut(s) 277, 309
BpmI CTGGAG 1 cut(s) 495
Bpu10I CCTNAGC 1 cut(s) 281
BsaI GGTCTC 1 cut(s) 524
BsaJI CCNNGG 3 cut(s) 27, 115, 728
BsaWI WCCGGW 1 cut(s) 479
Bsc4I CCNNNNNNNGG 1 cut(s) 282
Bse118I RCCGGY 2 cut(s) 59, 515
Bse3DI GCAATG 1 cut(s) 769
BseBI CCWGG 4 cut(s) 13, 277, 407, 534
BseDI CCNNGG 3 cut(s) 27, 115, 728
BseGI GGATG 3 cut(s) 304, 489, 758
BseLI CCNNNNNNNGG 1 cut(s) 282
BseMI GCAATG 1 cut(s) 769
BseMII CTCAG 3 cut(s) 272, 308, 802
BseRI GAGGAG 2 cut(s) 195, 298
BseSI GKGCMC 1 cut(s) 467
BseXI GCAGC 3 cut(s) 58, 284, 552
BsgI GTGCAG 2 cut(s) 291, 366
Bsh1236I CGCG 1 cut(s) 613
BshFI GGCC 2 cut(s) 155, 393
BsiHKAI GWGCWC 1 cut(s) 467
BsiSI CCGG 3 cut(s) 60, 480, 516
BslFI GGGAC 1 cut(s) 389
BslI CCNNNNNNNGG 1 cut(s) 282
BsmAI GTCTC 2 cut(s) 59, 524
BsmFI GGGAC 1 cut(s) 389
BsmI GAATGC 1 cut(s) 98
BsnI GGCC 2 cut(s) 155, 393
Bso31I GGTCTC 1 cut(s) 524
Bsp1286I GDGCHC 1 cut(s) 467
Bsp1407I TGTACA 1 cut(s) 420
Bsp143I GATC 4 cut(s) 104, 614, 732, 755
Bsp68I TCGCGA 1 cut(s) 613
BspACI CCGC 2 cut(s) 74, 108
BspANI GGCC 2 cut(s) 155, 393
BspCNI CTCAG 3 cut(s) 273, 307, 801
BspFNI CGCG 1 cut(s) 613
BspPI GGATC 4 cut(s) 99, 609, 740, 763
BspTNI GGTCTC 1 cut(s) 524
BsrDI GCAATG 1 cut(s) 769
BsrFI RCCGGY 2 cut(s) 59, 515
BsrGI TGTACA 1 cut(s) 420
BssAI RCCGGY 2 cut(s) 59, 515
BssECI CCNNGG 3 cut(s) 27, 115, 728
BssMI GATC 4 cut(s) 104, 614, 732, 755
BssT1I CCWWGG 1 cut(s) 115
Bst2UI CCWGG 4 cut(s) 13, 277, 407, 534
Bst4CI ACNGT 5 cut(s) 81, 191, 233, 626, 729
BstAUI TGTACA 1 cut(s) 420
BstC8I GCNNGC 3 cut(s) 387, 391, 776
BstDEI CTNAG 3 cut(s) 281, 294, 788
BstDSI CCRYGG 2 cut(s) 27, 728
BstEII GGTNACC 2 cut(s) 245, 305
BstF5I GGATG 3 cut(s) 304, 489, 758
BstFNI CGCG 1 cut(s) 613
BstKTI GATC 4 cut(s) 107, 617, 735, 758
BstMAI GTCTC 2 cut(s) 59, 524
BstMBI GATC 4 cut(s) 104, 614, 732, 755
BstMWI GCNNNNNNNGC 3 cut(s) 152, 462, 784
BstNI CCWGG 4 cut(s) 13, 277, 407, 534
BstNSI RCATGY 4 cut(s) 389, 421, 471, 557
BstPI GGTNACC 2 cut(s) 245, 305
BstSCI CCNGG 4 cut(s) 11, 275, 405, 532
BstSLI GKGCMC 1 cut(s) 467
BstUI CGCG 1 cut(s) 613
BstV1I GCAGC 3 cut(s) 58, 284, 552
BstX2I RGATCY 1 cut(s) 732
BstYI RGATCY 1 cut(s) 732
BsuI GTATCC 1 cut(s) 687
BsuRI GGCC 2 cut(s) 155, 393
BtgI CCRYGG 2 cut(s) 27, 728
BtsCI GGATG 3 cut(s) 304, 489, 758
BtsIMutI CAGTG 3 cut(s) 77, 196, 796
BtuMI TCGCGA 1 cut(s) 613
Cac8I GCNNGC 3 cut(s) 387, 391, 776
CaiI CAGNNNCTG 1 cut(s) 50
Cfr10I RCCGGY 2 cut(s) 59, 515
Csp6I GTAC 3 cut(s) 421, 505, 794
CviAII CATG 7 cut(s) 148, 386, 418, 460, 468, 554, 686
CviQI GTAC 3 cut(s) 421, 505, 794
DdeI CTNAG 3 cut(s) 281, 294, 788
DpnI GATC 4 cut(s) 106, 616, 734, 757
DpnII GATC 4 cut(s) 104, 614, 732, 755
Eco130I CCWWGG 1 cut(s) 115
Eco147I AGGCCT 1 cut(s) 155
Eco31I GGTCTC 1 cut(s) 524
Eco91I GGTNACC 2 cut(s) 245, 305
EcoO65I GGTNACC 2 cut(s) 245, 305
EcoRI GAATTC 2 cut(s) 365, 718
EcoRII CCWGG 4 cut(s) 11, 275, 405, 532
EcoT14I CCWWGG 1 cut(s) 115
EcoT22I ATGCAT 1 cut(s) 473
ErhI CCWWGG 1 cut(s) 115
FaeI CATG 7 cut(s) 151, 389, 421, 463, 471, 557, 689
FaqI GGGAC 1 cut(s) 389
FatI CATG 7 cut(s) 147, 385, 417, 459, 467, 553, 685
Fnu4HI GCNGC 4 cut(s) 72, 75, 273, 541
FokI GGATG 3 cut(s) 311, 476, 745
Fsp4HI GCNGC 4 cut(s) 72, 75, 273, 541
FspBI CTAG 2 cut(s) 116, 581
GluI GCNGC 4 cut(s) 72, 75, 273, 541
GsuI CTGGAG 1 cut(s) 495
HaeIII GGCC 2 cut(s) 155, 393
HapII CCGG 3 cut(s) 60, 480, 516
Hin1II CATG 7 cut(s) 151, 389, 421, 463, 471, 557, 689
HinfI GANTC 4 cut(s) 32, 40, 431, 483
HpaII CCGG 3 cut(s) 60, 480, 516
HphI GGTGA 2 cut(s) 74, 714
Hpy166II GTNNAC 5 cut(s) 31, 465, 507, 586, 794
Hpy188I TCNGA 3 cut(s) 52, 104, 693
Hpy188III TCNNGA 2 cut(s) 296, 612
Hpy8I GTNNAC 5 cut(s) 31, 465, 507, 586, 794
HpyAV CCTTC 5 cut(s) 145, 166, 404, 754, 777
HpyCH4III ACNGT 5 cut(s) 81, 191, 233, 626, 729
HpyCH4V TGCA 8 cut(s) 272, 347, 465, 471, 550, 557, 602, 778
HpyF10VI GCNNNNNNNGC 3 cut(s) 152, 462, 784
HpyF3I CTNAG 3 cut(s) 281, 294, 788
Hsp92II CATG 7 cut(s) 151, 389, 421, 463, 471, 557, 689
Kzo9I GATC 4 cut(s) 104, 614, 732, 755
LmnI GCTCC 1 cut(s) 182
Lsp1109I GCAGC 3 cut(s) 58, 284, 552
MaeI CTAG 2 cut(s) 116, 581
MaeIII GTNAC 3 cut(s) 119, 245, 305
MalI GATC 4 cut(s) 106, 616, 734, 757
MboI GATC 4 cut(s) 104, 614, 732, 755
MboII GAAGA 1 cut(s) 727
MflI RGATCY 1 cut(s) 732
MhlI GDGCHC 1 cut(s) 467
MluCI AATT 5 cut(s) 257, 365, 592, 718, 800
MlyI GAGTC 1 cut(s) 26
MmeI TCCRAC 1 cut(s) 671
MnlI CCTC 9 cut(s) 100, 173, 276, 343, 404, 503, 530, 619, 629
Mph1103I ATGCAT 1 cut(s) 473
MroXI GAANNNNTTC 1 cut(s) 155
MseI TTAA 1 cut(s) 474
MslI CAYNNNNRTG 1 cut(s) 727
MspA1I CMGCKG 1 cut(s) 71
MspI CCGG 3 cut(s) 60, 480, 516
MspR9I CCNGG 4 cut(s) 13, 277, 407, 534
Mva1269I GAATGC 1 cut(s) 98
MvaI CCWGG 4 cut(s) 13, 277, 407, 534
MvnI CGCG 1 cut(s) 613
MwoI GCNNNNNNNGC 3 cut(s) 152, 462, 784
NdeII GATC 4 cut(s) 104, 614, 732, 755
NlaIII CATG 7 cut(s) 151, 389, 421, 463, 471, 557, 689
NruI TCGCGA 1 cut(s) 613
NsiI ATGCAT 1 cut(s) 473
NspI RCATGY 4 cut(s) 389, 421, 471, 557
OliI CACNNNNGTG 1 cut(s) 727
PaeI GCATGC 1 cut(s) 389
PceI AGGCCT 1 cut(s) 155
PciI ACATGT 1 cut(s) 417
PcsI WCGNNNNNNNCGW 2 cut(s) 610, 697
PctI GAATGC 1 cut(s) 98
PdmI GAANNNNTTC 1 cut(s) 155
PfeI GAWTC 3 cut(s) 40, 431, 483
PfoI TCCNGGA 1 cut(s) 405
PkrI GCNGC 4 cut(s) 73, 76, 274, 542
PleI GAGTC 1 cut(s) 26
PpsI GAGTC 1 cut(s) 26
PscI ACATGT 1 cut(s) 417
PshBI ATTAAT 1 cut(s) 474
Psp6I CCWGG 4 cut(s) 11, 275, 405, 532
PspEI GGTNACC 2 cut(s) 245, 305
PspGI CCWGG 4 cut(s) 11, 275, 405, 532
PstNI CAGNNNCTG 1 cut(s) 50
PsuI RGATCY 1 cut(s) 732
PvuII CAGCTG 1 cut(s) 71
RruI TCGCGA 1 cut(s) 613
RsaI GTAC 3 cut(s) 422, 506, 795
RsaNI GTAC 3 cut(s) 421, 505, 794
RseI CAYNNNNRTG 1 cut(s) 727
SaqAI TTAA 1 cut(s) 474
SatI GCNGC 4 cut(s) 72, 75, 273, 541
Sau3AI GATC 4 cut(s) 104, 614, 732, 755
SchI GAGTC 1 cut(s) 26
ScrFI CCNGG 4 cut(s) 13, 277, 407, 534
SduI GDGCHC 1 cut(s) 467
SmiMI CAYNNNNRTG 1 cut(s) 727
SpeI ACTAGT 1 cut(s) 580
SphI GCATGC 1 cut(s) 389
Sse9I AATT 5 cut(s) 257, 365, 592, 718, 800
SseBI AGGCCT 1 cut(s) 155
SsiI CCGC 2 cut(s) 74, 108
SspMI CTAG 2 cut(s) 116, 581
StuI AGGCCT 1 cut(s) 155
StyD4I CCNGG 4 cut(s) 11, 275, 405, 532
StyI CCWWGG 1 cut(s) 115
TaaI ACNGT 5 cut(s) 81, 191, 233, 626, 729
TaqI TCGA 4 cut(s) 159, 240, 501, 563
TasI AATT 5 cut(s) 257, 365, 592, 718, 800
TatI WGTACW 3 cut(s) 420, 504, 793
TauI GCSGC 1 cut(s) 77
TfiI GAWTC 3 cut(s) 40, 431, 483
Tru1I TTAA 1 cut(s) 474
Tru9I TTAA 1 cut(s) 474
TscAI CASTG 3 cut(s) 84, 196, 796
TseI GCWGC 3 cut(s) 71, 272, 540
TspDTI ATGAA 4 cut(s) 164, 414, 448, 558
TspRI CASTG 3 cut(s) 84, 196, 796
VneI GTGCAC 1 cut(s) 463
VspI ATTAAT 1 cut(s) 474
XapI RAATTY 2 cut(s) 365, 718
XceI RCATGY 4 cut(s) 389, 421, 471, 557
XcmI CCANNNNNNNNNTGG 1 cut(s) 668
XmaJI CCTAGG 1 cut(s) 115
XmnI GAANNNNTTC 1 cut(s) 155
XspI CTAG 2 cut(s) 116, 581
Zsp2I ATGCAT 1 cut(s) 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.