Rh6BG157000

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0004434
Physical Location & Seq
Forward (+)
25353641 .. 25357065
3425 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG157000.1

Sequence Viewer

Length: 1023 bp
ATGGACACAGCCAGGACTGTATATGCCGTGGACTCGCCGAATCCAGCTTCTGATGTTGCCGGTGAGACAGCTGCGGCGCTGTCAGCTTCCTCAATGGCATTCCGATCCTCTGACCCTAGTTACGCAGACACATTGTTGCGAAATGCCATGAAGGCCTTCGAGTTTGCTGATACTTACAGAGGAGCTTACAGTGATAATGCCAATGTTAGAGATGGTGTCTGCCCATTTTACTGTGATTTCGATGGTTACCAAGACGAATTGCTATGGAGTGCAGCCTGGCTGAGGAGAGTCACTCAGGATGGTGGTTACCTTGACTACATACAAAACAATGGCAAAACTCTTGGTGCAGAGGACAATATAAACGAATTTGGCTGGGACAACAAGCATGCTGGCCTCAATGTTCTAATCTCCAAGGAAGTCCTGGAAGGCAACATGTACAACCTTGAATCCTACAAATCATCGGCTGATAGCTTCATATGCACATTAATACCGGAGTCATCATCCTCCCACATCGAGTACACTCCAGCCGGTCTCATCTACAAGCCAGGAGGCAGCAATCTGCAACATGTAACTTCCATTTCATTTCTACTACTAGTTTATGCCAATTACCTTGCACGAACCTCGCGATCCCTCAGCTGTGGTAACATAGATGTCAGTCCAGCAACGCTTCGCCAAATCGCCAAAAAGCAGGTTGATTACATTTTGGGTGATAATCCAATGGGTTTGTCATACATGGTCGGATACAGCGACCATTATCCGCAGCGAATTCACCATCGTGGATCTTCTTTACCATCTATCAAGGATCATCCCCAACCCATTGCTTGCAAGGAAGGCTCAGTGTACTTCAATTCGTCAAACCCTAACCCTAACCCCCTTGTGGGAGCTCTAGTAGGAGGACCTGGAGAAGATGATGTCTATGAGGATGATCGCGCTGATTTTAGGAAGTCCAAACCGACCACATACATTAATGCACCATTTGTTGGTGTTTTGGCATACTTTGTGGCTAATCCCAGTCCAAACTAG

Protein Analysis

340

Amino Acids

37.19

Weight (kDa)

4.91

Isoelectric Point (pI)

48.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 1 - 330 2.2e-96 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 679
AccB7I CCANNNNNTGG 1 cut(s) 980
AccII CGCG 2 cut(s) 625, 930
AciI CCGC 2 cut(s) 74, 758
AclWI GGATC 4 cut(s) 99, 621, 787, 810
AcsI RAATTY 2 cut(s) 365, 765
AfaI GTAC 3 cut(s) 437, 518, 842
AfiI CCNNNNNNNGG 4 cut(s) 282, 877, 878, 980
AflIII ACRYGT 2 cut(s) 432, 565
AgsI TTSAA 2 cut(s) 446, 847
AhlI ACTAGT 1 cut(s) 592
AjnI CCWGG 5 cut(s) 11, 275, 420, 544, 898
AleI CACNNNNGTG 1 cut(s) 774
AluBI AGCT 7 cut(s) 47, 71, 86, 185, 471, 636, 884
AluI AGCT 7 cut(s) 47, 71, 86, 185, 471, 636, 884
Alw21I GWGCWC 1 cut(s) 886
Alw26I GTCTC 2 cut(s) 59, 536
AlwI GGATC 4 cut(s) 99, 621, 787, 810
AlwNI CAGNNNCTG 1 cut(s) 50
AoxI GGCC 2 cut(s) 153, 391
ApeKI GCWGC 4 cut(s) 71, 272, 552, 760
ApoI RAATTY 2 cut(s) 365, 765
AseI ATTAAT 2 cut(s) 485, 966
Asp700I GAANNNNTTC 1 cut(s) 155
AspLEI GCGC 2 cut(s) 79, 932
AspS9I GGNCC 1 cut(s) 896
AsuHPI GGTGA 3 cut(s) 74, 719, 761
AvaII GGWCC 1 cut(s) 896
BanII GRGCYC 1 cut(s) 886
Bbv12I GWGCWC 1 cut(s) 886
BbvCI CCTCAGC 2 cut(s) 281, 632
BbvI GCAGC 4 cut(s) 58, 284, 564, 772
BccI CCATC 5 cut(s) 206, 236, 293, 780, 799
BceAI ACGGC 1 cut(s) 11
BcgI CGANNNNNNTGC 2 cut(s) 603, 637
BciT130I CCWGG 5 cut(s) 13, 277, 422, 546, 900
BciVI GTATCC 1 cut(s) 734
BcoDI GTCTC 2 cut(s) 59, 536
BcuI ACTAGT 1 cut(s) 592
BfaI CTAG 4 cut(s) 117, 593, 887, 1021
BfoI RGCGCY 1 cut(s) 80
BfuAI ACCTGC 1 cut(s) 679
BfuI GTATCC 1 cut(s) 734
BglI GCCNNNNNGGC 1 cut(s) 152
BisI GCNGC 5 cut(s) 72, 75, 273, 553, 761
BlsI GCNGC 5 cut(s) 73, 76, 274, 554, 762
Bme1390I CCNGG 5 cut(s) 13, 277, 422, 546, 900
Bme18I GGWCC 1 cut(s) 896
BmgT120I GGNCC 1 cut(s) 896
BmrFI CCNGG 5 cut(s) 13, 277, 422, 546, 900
BmrI ACTGGG 1 cut(s) 1005
BmuI ACTGGG 1 cut(s) 1005
BplI GAGNNNNNCTC 2 cut(s) 277, 309
BpmI CTGGAG 2 cut(s) 507, 921
Bpu10I CCTNAGC 2 cut(s) 281, 632
BsaI GGTCTC 1 cut(s) 536
BsaJI CCNNGG 2 cut(s) 27, 411
BsaWI WCCGGW 1 cut(s) 490
BsaXI ACNNNNNCTCC 2 cut(s) 873, 903
Bsc4I CCNNNNNNNGG 4 cut(s) 282, 877, 878, 980
Bse118I RCCGGY 2 cut(s) 59, 527
Bse1I ACTGG 1 cut(s) 1011
Bse3DI GCAATG 1 cut(s) 816
BseBI CCWGG 5 cut(s) 13, 277, 422, 546, 900
BseDI CCNNGG 2 cut(s) 27, 411
BseGI GGATG 4 cut(s) 304, 500, 805, 928
BseLI CCNNNNNNNGG 4 cut(s) 282, 877, 878, 980
BseMI GCAATG 1 cut(s) 816
BseMII CTCAG 4 cut(s) 272, 308, 646, 849
BseNI ACTGG 1 cut(s) 1011
BseRI GAGGAG 2 cut(s) 195, 298
BseXI GCAGC 4 cut(s) 58, 284, 564, 772
BseYI CCCAGC 1 cut(s) 372
BsgI GTGCAG 2 cut(s) 291, 366
Bsh1236I CGCG 2 cut(s) 625, 930
BshFI GGCC 2 cut(s) 155, 393
BsiHKAI GWGCWC 1 cut(s) 886
BsiSI CCGG 3 cut(s) 60, 491, 528
BslFI GGGAC 1 cut(s) 389
BslI CCNNNNNNNGG 4 cut(s) 282, 877, 878, 980
BsmAI GTCTC 2 cut(s) 59, 536
BsmFI GGGAC 1 cut(s) 389
BsmI GAATGC 1 cut(s) 98
BsnI GGCC 2 cut(s) 155, 393
Bso31I GGTCTC 1 cut(s) 536
Bsp1286I GDGCHC 1 cut(s) 886
Bsp1407I TGTACA 1 cut(s) 435
Bsp143I GATC 5 cut(s) 104, 626, 779, 802, 925
Bsp68I TCGCGA 1 cut(s) 625
BspACI CCGC 2 cut(s) 74, 758
BspANI GGCC 2 cut(s) 155, 393
BspCNI CTCAG 4 cut(s) 273, 307, 645, 848
BspFNI CGCG 2 cut(s) 625, 930
BspMI ACCTGC 1 cut(s) 679
BspPI GGATC 4 cut(s) 99, 621, 787, 810
BspTNI GGTCTC 1 cut(s) 536
BsrDI GCAATG 1 cut(s) 816
BsrFI RCCGGY 2 cut(s) 59, 527
BsrGI TGTACA 1 cut(s) 435
BsrI ACTGG 1 cut(s) 1011
BssAI RCCGGY 2 cut(s) 59, 527
BssECI CCNNGG 2 cut(s) 27, 411
BssMI GATC 5 cut(s) 104, 626, 779, 802, 925
BssT1I CCWWGG 1 cut(s) 411
Bst2UI CCWGG 5 cut(s) 13, 277, 422, 546, 900
Bst4CI ACNGT 3 cut(s) 19, 191, 233
BstAUI TGTACA 1 cut(s) 435
BstC8I GCNNGC 3 cut(s) 387, 391, 823
BstDEI CTNAG 4 cut(s) 281, 294, 632, 835
BstDSI CCRYGG 1 cut(s) 27
BstEII GGTNACC 2 cut(s) 245, 305
BstENI CCTNNNNNAGG 1 cut(s) 280
BstF5I GGATG 4 cut(s) 304, 500, 805, 928
BstFNI CGCG 2 cut(s) 625, 930
BstH2I RGCGCY 1 cut(s) 80
BstHHI GCGC 2 cut(s) 79, 932
BstKTI GATC 5 cut(s) 107, 629, 782, 805, 928
BstMAI GTCTC 2 cut(s) 59, 536
BstMBI GATC 5 cut(s) 104, 626, 779, 802, 925
BstMWI GCNNNNNNNGC 4 cut(s) 83, 152, 477, 831
BstNI CCWGG 5 cut(s) 13, 277, 422, 546, 900
BstNSI RCATGY 3 cut(s) 389, 436, 569
BstPI GGTNACC 2 cut(s) 245, 305
BstSCI CCNGG 5 cut(s) 11, 275, 420, 544, 898
BstUI CGCG 2 cut(s) 625, 930
BstV1I GCAGC 4 cut(s) 58, 284, 564, 772
BstX2I RGATCY 1 cut(s) 779
BstYI RGATCY 1 cut(s) 779
BsuI GTATCC 1 cut(s) 734
BsuRI GGCC 2 cut(s) 155, 393
BtgI CCRYGG 1 cut(s) 27
BtsCI GGATG 4 cut(s) 304, 500, 805, 928
BtsIMutI CAGTG 2 cut(s) 196, 843
BtuMI TCGCGA 1 cut(s) 625
BveI ACCTGC 1 cut(s) 679
Cac8I GCNNGC 3 cut(s) 387, 391, 823
CaiI CAGNNNCTG 1 cut(s) 50
CfoI GCGC 2 cut(s) 79, 932
Cfr10I RCCGGY 2 cut(s) 59, 527
Cfr13I GGNCC 1 cut(s) 896
Csp6I GTAC 3 cut(s) 436, 517, 841
CviAII CATG 5 cut(s) 148, 386, 433, 566, 733
CviQI GTAC 3 cut(s) 436, 517, 841
DdeI CTNAG 4 cut(s) 281, 294, 632, 835
DpnI GATC 5 cut(s) 106, 628, 781, 804, 927
DpnII GATC 5 cut(s) 104, 626, 779, 802, 925
Ecl136II GAGCTC 1 cut(s) 884
Eco130I CCWWGG 1 cut(s) 411
Eco147I AGGCCT 1 cut(s) 155
Eco24I GRGCYC 1 cut(s) 886
Eco31I GGTCTC 1 cut(s) 536
Eco47I GGWCC 1 cut(s) 896
Eco53kI GAGCTC 1 cut(s) 884
Eco91I GGTNACC 2 cut(s) 245, 305
EcoICRI GAGCTC 1 cut(s) 884
EcoNI CCTNNNNNAGG 1 cut(s) 280
EcoO109I RGGNCCY 1 cut(s) 896
EcoO65I GGTNACC 2 cut(s) 245, 305
EcoRI GAATTC 1 cut(s) 765
EcoRII CCWGG 5 cut(s) 11, 275, 420, 544, 898
EcoT14I CCWWGG 1 cut(s) 411
EcoT38I GRGCYC 1 cut(s) 886
ErhI CCWWGG 1 cut(s) 411
FaeI CATG 5 cut(s) 151, 389, 436, 569, 736
FaqI GGGAC 1 cut(s) 389
FatI CATG 5 cut(s) 147, 385, 432, 565, 732
FauNDI CATATG 1 cut(s) 476
Fnu4HI GCNGC 5 cut(s) 72, 75, 273, 553, 761
FokI GGATG 4 cut(s) 311, 487, 792, 935
FriOI GRGCYC 1 cut(s) 886
Fsp4HI GCNGC 5 cut(s) 72, 75, 273, 553, 761
FspBI CTAG 4 cut(s) 117, 593, 887, 1021
GlaI GCGC 2 cut(s) 78, 931
GluI GCNGC 5 cut(s) 72, 75, 273, 553, 761
GsaI CCCAGC 1 cut(s) 376
GsuI CTGGAG 2 cut(s) 507, 921
HaeII RGCGCY 1 cut(s) 80
HaeIII GGCC 2 cut(s) 155, 393
HapII CCGG 3 cut(s) 60, 491, 528
HhaI GCGC 2 cut(s) 79, 932
Hin1II CATG 5 cut(s) 151, 389, 436, 569, 736
Hin6I GCGC 2 cut(s) 77, 930
HinP1I GCGC 2 cut(s) 77, 930
HinfI GANTC 5 cut(s) 32, 40, 288, 446, 494
HpaII CCGG 3 cut(s) 60, 491, 528
HphI GGTGA 3 cut(s) 74, 719, 761
Hpy166II GTNNAC 3 cut(s) 31, 519, 841
Hpy188I TCNGA 4 cut(s) 52, 104, 112, 740
Hpy188III TCNNGA 2 cut(s) 296, 624
Hpy8I GTNNAC 3 cut(s) 31, 519, 841
HpyAV CCTTC 4 cut(s) 145, 166, 419, 824
HpyCH4III ACNGT 3 cut(s) 19, 191, 233
HpyCH4V TGCA 7 cut(s) 272, 347, 480, 562, 614, 825, 971
HpyF10VI GCNNNNNNNGC 4 cut(s) 83, 152, 477, 831
HpyF3I CTNAG 4 cut(s) 281, 294, 632, 835
Hsp92II CATG 5 cut(s) 151, 389, 436, 569, 736
HspAI GCGC 2 cut(s) 77, 930
Kzo9I GATC 5 cut(s) 104, 626, 779, 802, 925
LmnI GCTCC 2 cut(s) 182, 881
Lsp1109I GCAGC 4 cut(s) 58, 284, 564, 772
MaeI CTAG 4 cut(s) 117, 593, 887, 1021
MaeIII GTNAC 6 cut(s) 119, 245, 289, 305, 568, 641
MalI GATC 5 cut(s) 106, 628, 781, 804, 927
MboI GATC 5 cut(s) 104, 626, 779, 802, 925
MboII GAAGA 2 cut(s) 774, 917
MflI RGATCY 1 cut(s) 779
MhlI GDGCHC 1 cut(s) 886
MluCI AATT 5 cut(s) 257, 365, 604, 765, 847
MlyI GAGTC 3 cut(s) 26, 297, 503
MmeI TCCRAC 1 cut(s) 718
MroXI GAANNNNTTC 1 cut(s) 155
MseI TTAA 2 cut(s) 485, 966
MslI CAYNNNNRTG 1 cut(s) 774
MspA1I CMGCKG 2 cut(s) 71, 636
MspI CCGG 3 cut(s) 60, 491, 528
MspR9I CCNGG 5 cut(s) 13, 277, 422, 546, 900
Mva1269I GAATGC 1 cut(s) 98
MvaI CCWGG 5 cut(s) 13, 277, 422, 546, 900
MvnI CGCG 2 cut(s) 625, 930
MwoI GCNNNNNNNGC 4 cut(s) 83, 152, 477, 831
NdeI CATATG 1 cut(s) 476
NdeII GATC 5 cut(s) 104, 626, 779, 802, 925
NlaIII CATG 5 cut(s) 151, 389, 436, 569, 736
NmuCI GTSAC 1 cut(s) 289
NruI TCGCGA 1 cut(s) 625
NspI RCATGY 3 cut(s) 389, 436, 569
OliI CACNNNNGTG 1 cut(s) 774
PaeI GCATGC 1 cut(s) 389
PceI AGGCCT 1 cut(s) 155
PciI ACATGT 2 cut(s) 432, 565
PcsI WCGNNNNNNNCGW 2 cut(s) 622, 744
PctI GAATGC 1 cut(s) 98
PdmI GAANNNNTTC 1 cut(s) 155
PfeI GAWTC 2 cut(s) 40, 446
PflMI CCANNNNNTGG 1 cut(s) 980
PfoI TCCNGGA 1 cut(s) 420
PkrI GCNGC 5 cut(s) 73, 76, 274, 554, 762
PleI GAGTC 3 cut(s) 26, 296, 502
PpsI GAGTC 3 cut(s) 26, 296, 502
PpuMI RGGWCCY 1 cut(s) 896
PscI ACATGT 2 cut(s) 432, 565
PshBI ATTAAT 2 cut(s) 485, 966
Psp124BI GAGCTC 1 cut(s) 886
Psp5II RGGWCCY 1 cut(s) 896
Psp6I CCWGG 5 cut(s) 11, 275, 420, 544, 898
PspEI GGTNACC 2 cut(s) 245, 305
PspFI CCCAGC 1 cut(s) 372
PspGI CCWGG 5 cut(s) 11, 275, 420, 544, 898
PspPI GGNCC 1 cut(s) 896
PspPPI RGGWCCY 1 cut(s) 896
PstNI CAGNNNCTG 1 cut(s) 50
PsuI RGATCY 1 cut(s) 779
PvuII CAGCTG 2 cut(s) 71, 636
RruI TCGCGA 1 cut(s) 625
RsaI GTAC 3 cut(s) 437, 518, 842
RsaNI GTAC 3 cut(s) 436, 517, 841
RseI CAYNNNNRTG 1 cut(s) 774
SacI GAGCTC 1 cut(s) 886
SaqAI TTAA 2 cut(s) 485, 966
SatI GCNGC 5 cut(s) 72, 75, 273, 553, 761
Sau3AI GATC 5 cut(s) 104, 626, 779, 802, 925
Sau96I GGNCC 1 cut(s) 896
SchI GAGTC 3 cut(s) 26, 297, 503
ScrFI CCNGG 5 cut(s) 13, 277, 422, 546, 900
SduI GDGCHC 1 cut(s) 886
SinI GGWCC 1 cut(s) 896
SmiMI CAYNNNNRTG 1 cut(s) 774
SpeI ACTAGT 1 cut(s) 592
SphI GCATGC 1 cut(s) 389
Sse9I AATT 5 cut(s) 257, 365, 604, 765, 847
SseBI AGGCCT 1 cut(s) 155
SsiI CCGC 2 cut(s) 74, 758
SspMI CTAG 4 cut(s) 117, 593, 887, 1021
SstI GAGCTC 1 cut(s) 886
StuI AGGCCT 1 cut(s) 155
StyD4I CCNGG 5 cut(s) 11, 275, 420, 544, 898
StyI CCWWGG 1 cut(s) 411
TaaI ACNGT 3 cut(s) 19, 191, 233
TaqI TCGA 3 cut(s) 159, 240, 513
TasI AATT 5 cut(s) 257, 365, 604, 765, 847
TatI WGTACW 3 cut(s) 435, 516, 840
TauI GCSGC 1 cut(s) 77
TfiI GAWTC 2 cut(s) 40, 446
Tru1I TTAA 2 cut(s) 485, 966
Tru9I TTAA 2 cut(s) 485, 966
TscAI CASTG 2 cut(s) 196, 843
TseFI GTSAC 1 cut(s) 289
TseI GCWGC 4 cut(s) 71, 272, 552, 760
Tsp45I GTSAC 1 cut(s) 289
TspDTI ATGAA 3 cut(s) 164, 463, 570
TspRI CASTG 2 cut(s) 196, 843
Van91I CCANNNNNTGG 1 cut(s) 980
VpaK11BI GGWCC 1 cut(s) 896
VspI ATTAAT 2 cut(s) 485, 966
XagI CCTNNNNNAGG 1 cut(s) 280
XapI RAATTY 2 cut(s) 365, 765
XceI RCATGY 3 cut(s) 389, 436, 569
XcmI CCANNNNNNNNNTGG 1 cut(s) 418
XmnI GAANNNNTTC 1 cut(s) 155
XspI CTAG 4 cut(s) 117, 593, 887, 1021
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.