MD10G1186600.v1.1

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0004434
Physical Location & Seq
Forward (+)
28085557 .. 28086018
462 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1186600.v1.1.491

Sequence Viewer

Length: 462 bp
ATGGGCACGTCACGCACCGTGTACAAGATCGATAAGAACCATCCGGGGTCTGACGTGGCAGGCGAAACAGCCACTGCTCTCGCCGTTGCTTCTATCGTGTTCAGATCACTTGACCCAGCTTACTTTAGATTCCTCCTCAATCGAGCCGTTAGGGTGTTCGAGTTTGCTGACAGGCACCGGGGTGCGTACAGCGTCAGCCTGCACCATGTCGTGTGCCCTTTTTACTGCGACGTGAACGGCTTCCAGCATGAGTTACTGTGGGGAGCAGTGTGGCTGCACAAGGCATCGAAGAGGCGTGTATACAGAAAATATATAGTGAAGAACGAGGTGGTGTTGCGAGCAGGTGATACGATTAATGAGTTTGGTTGGGACAACAAACATGCTTGGATTAACGTCCTCATTTCCACGGGCCTCCGTATTTGGGAGCGGTGGGGGTCAGAGTTCTTTGAACTATCTTTTTAG

Protein Analysis

154

Amino Acids

17.72

Weight (kDa)

9.39

Isoelectric Point (pI)

29.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 3 - 136 2.6e-34 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 332
Acc36I ACCTGC 1 cut(s) 332
AccB1I GGYRCC 1 cut(s) 174
AccBSI CCGCTC 1 cut(s) 427
AccI GTMKAC 1 cut(s) 300
AciI CCGC 1 cut(s) 427
AfaI GTAC 2 cut(s) 23, 188
AfiI CCNNNNNNNGG 1 cut(s) 421
AgsI TTSAA 1 cut(s) 449
AjiI CACGTC 3 cut(s) 9, 55, 232
AleI CACNNNNGTG 1 cut(s) 180
AluBI AGCT 1 cut(s) 119
AluI AGCT 1 cut(s) 119
AlwNI CAGNNNCTG 1 cut(s) 74
AoxI GGCC 1 cut(s) 409
ApeKI GCWGC 1 cut(s) 274
AseI ATTAAT 1 cut(s) 354
Asp700I GAANNNNTTC 1 cut(s) 239
AspS9I GGNCC 1 cut(s) 409
AsuC2I CCSGG 2 cut(s) 45, 179
AsuHPI GGTGA 1 cut(s) 356
BaeGI GKGCMC 2 cut(s) 8, 218
BanI GGYRCC 1 cut(s) 174
BbvI GCAGC 1 cut(s) 261
BccI CCATC 1 cut(s) 48
BceAI ACGGC 3 cut(s) 68, 131, 253
BcnI CCSGG 2 cut(s) 45, 179
BfuAI ACCTGC 1 cut(s) 332
BisI GCNGC 1 cut(s) 275
BlsI GCNGC 1 cut(s) 276
Bme1390I CCNGG 2 cut(s) 45, 179
BmgBI CACGTC 3 cut(s) 9, 55, 232
BmgT120I GGNCC 1 cut(s) 409
BmiI GGNNCC 1 cut(s) 176
BmrFI CCNGG 2 cut(s) 45, 179
BmsI GCATC 1 cut(s) 293
BpuMI CCSGG 2 cut(s) 45, 179
Bsa29I ATCGAT 1 cut(s) 30
BsaJI CCNNGG 3 cut(s) 44, 178, 405
Bsc4I CCNNNNNNNGG 1 cut(s) 421
BseCI ATCGAT 1 cut(s) 30
BseDI CCNNGG 3 cut(s) 44, 178, 405
BseGI GGATG 1 cut(s) 40
BseLI CCNNNNNNNGG 1 cut(s) 421
BseRI GAGGAG 1 cut(s) 125
BseSI GKGCMC 2 cut(s) 8, 218
BseXI GCAGC 1 cut(s) 261
BseYI CCCAGC 1 cut(s) 115
BsgI GTGCAG 2 cut(s) 185, 260
BshFI GGCC 1 cut(s) 411
BshNI GGYRCC 1 cut(s) 174
BshVI ATCGAT 1 cut(s) 30
BsiSI CCGG 2 cut(s) 44, 178
BslFI GGGAC 1 cut(s) 383
BslI CCNNNNNNNGG 1 cut(s) 421
BsmFI GGGAC 1 cut(s) 383
BsnI GGCC 1 cut(s) 411
Bsp1286I GDGCHC 2 cut(s) 8, 218
Bsp1407I TGTACA 1 cut(s) 21
Bsp143I GATC 2 cut(s) 27, 104
BspACI CCGC 1 cut(s) 427
BspANI GGCC 1 cut(s) 411
BspDI ATCGAT 1 cut(s) 30
BspLI GGNNCC 1 cut(s) 176
BspMI ACCTGC 1 cut(s) 332
BspT107I GGYRCC 1 cut(s) 174
BsrBI CCGCTC 1 cut(s) 427
BsrGI TGTACA 1 cut(s) 21
BssECI CCNNGG 3 cut(s) 44, 178, 405
BssMI GATC 2 cut(s) 27, 104
BssNAI GTATAC 1 cut(s) 301
Bst1107I GTATAC 1 cut(s) 301
Bst4CI ACNGT 2 cut(s) 19, 258
Bst6I CTCTTC 1 cut(s) 284
BstAUI TGTACA 1 cut(s) 21
BstC8I GCNNGC 3 cut(s) 61, 200, 339
BstDSI CCRYGG 1 cut(s) 405
BstF5I GGATG 1 cut(s) 40
BstKTI GATC 2 cut(s) 30, 107
BstMBI GATC 2 cut(s) 27, 104
BstMWI GCNNNNNNNGC 1 cut(s) 12
BstNSI RCATGY 1 cut(s) 383
BstSCI CCNGG 2 cut(s) 43, 177
BstSLI GKGCMC 2 cut(s) 8, 218
BstV1I GCAGC 1 cut(s) 261
BstZ17I GTATAC 1 cut(s) 301
Bsu15I ATCGAT 1 cut(s) 30
BsuRI GGCC 1 cut(s) 411
BsuTUI ATCGAT 1 cut(s) 30
BtgI CCRYGG 1 cut(s) 405
BtrI CACGTC 3 cut(s) 9, 55, 232
BtsCI GGATG 1 cut(s) 40
BtsI GCAGTG 2 cut(s) 72, 273
BtsIMutI CAGTG 2 cut(s) 72, 273
BveI ACCTGC 1 cut(s) 332
Cac8I GCNNGC 3 cut(s) 61, 200, 339
CaiI CAGNNNCTG 1 cut(s) 74
Cfr13I GGNCC 1 cut(s) 409
ClaI ATCGAT 1 cut(s) 30
CseI GACGC 1 cut(s) 181
Csp6I GTAC 2 cut(s) 22, 187
CviAII CATG 3 cut(s) 206, 248, 380
CviJI RGCY 7 cut(s) 71, 119, 146, 198, 240, 274, 411
CviKI_1 RGCY 7 cut(s) 71, 119, 146, 198, 240, 274, 411
CviQI GTAC 2 cut(s) 22, 187
DpnI GATC 2 cut(s) 29, 106
DpnII GATC 2 cut(s) 27, 104
Eam1104I CTCTTC 1 cut(s) 284
EarI CTCTTC 1 cut(s) 284
FaeI CATG 3 cut(s) 209, 251, 383
FaiI YATR 6 cut(s) 207, 249, 301, 312, 314, 381
FaqI GGGAC 1 cut(s) 383
FatI CATG 3 cut(s) 205, 247, 379
FblI GTMKAC 1 cut(s) 300
Fnu4HI GCNGC 1 cut(s) 275
FokI GGATG 1 cut(s) 27
Fsp4HI GCNGC 1 cut(s) 275
GluI GCNGC 1 cut(s) 275
GsaI CCCAGC 1 cut(s) 119
HaeIII GGCC 1 cut(s) 411
HapII CCGG 2 cut(s) 44, 178
HgaI GACGC 1 cut(s) 181
Hin1II CATG 3 cut(s) 209, 251, 383
HinfI GANTC 1 cut(s) 129
HpaII CCGG 2 cut(s) 44, 178
HphI GGTGA 1 cut(s) 356
Hpy166II GTNNAC 3 cut(s) 22, 235, 301
Hpy188I TCNGA 3 cut(s) 52, 104, 439
Hpy8I GTNNAC 3 cut(s) 22, 235, 301
Hpy99I CGWCG 1 cut(s) 233
HpyCH4III ACNGT 2 cut(s) 19, 258
HpyCH4IV ACGT 4 cut(s) 8, 54, 231, 393
HpyCH4V TGCA 2 cut(s) 202, 277
HpyF10VI GCNNNNNNNGC 1 cut(s) 12
HpySE526I ACGT 4 cut(s) 8, 54, 231, 393
Hsp92II CATG 3 cut(s) 209, 251, 383
Kzo9I GATC 2 cut(s) 27, 104
LmnI GCTCC 2 cut(s) 263, 424
LpnPI CCDG 8 cut(s) 45, 57, 129, 157, 191, 212, 257, 327
Lsp1109I GCAGC 1 cut(s) 261
LweI GCATC 1 cut(s) 293
MaeII ACGT 4 cut(s) 8, 54, 231, 393
MaeIII GTNAC 2 cut(s) 9, 252
MalI GATC 2 cut(s) 29, 106
MbiI CCGCTC 1 cut(s) 427
MboI GATC 2 cut(s) 27, 104
MboII GAAGA 2 cut(s) 301, 331
MhlI GDGCHC 2 cut(s) 8, 218
MnlI CCTC 6 cut(s) 143, 146, 285, 319, 407, 422
MroXI GAANNNNTTC 1 cut(s) 239
MseI TTAA 2 cut(s) 354, 390
MslI CAYNNNNRTG 1 cut(s) 180
MspI CCGG 2 cut(s) 44, 178
MspR9I CCNGG 2 cut(s) 45, 179
MwoI GCNNNNNNNGC 1 cut(s) 12
NciI CCSGG 2 cut(s) 45, 179
NdeII GATC 2 cut(s) 27, 104
NlaIII CATG 3 cut(s) 209, 251, 383
NlaIV GGNNCC 1 cut(s) 176
NmuCI GTSAC 1 cut(s) 9
NspI RCATGY 1 cut(s) 383
OliI CACNNNNGTG 1 cut(s) 180
PaqCI CACCTGC 1 cut(s) 332
PcsI WCGNNNNNNNCGW 1 cut(s) 60
PdmI GAANNNNTTC 1 cut(s) 239
PfeI GAWTC 1 cut(s) 129
PkrI GCNGC 1 cut(s) 276
PshBI ATTAAT 1 cut(s) 354
PspFI CCCAGC 1 cut(s) 115
PspN4I GGNNCC 1 cut(s) 176
PspPI GGNCC 1 cut(s) 409
PstNI CAGNNNCTG 1 cut(s) 74
RsaI GTAC 2 cut(s) 23, 188
RsaNI GTAC 2 cut(s) 22, 187
RseI CAYNNNNRTG 1 cut(s) 180
SaqAI TTAA 2 cut(s) 354, 390
SatI GCNGC 1 cut(s) 275
Sau3AI GATC 2 cut(s) 27, 104
Sau96I GGNCC 1 cut(s) 409
ScrFI CCNGG 2 cut(s) 45, 179
SduI GDGCHC 2 cut(s) 8, 218
SetI ASST 7 cut(s) 11, 57, 121, 234, 330, 346, 396
SfaNI GCATC 1 cut(s) 293
SmiMI CAYNNNNRTG 1 cut(s) 180
SsiI CCGC 1 cut(s) 427
StyD4I CCNGG 2 cut(s) 43, 177
TaaI ACNGT 2 cut(s) 19, 258
TaiI ACGT 4 cut(s) 11, 57, 234, 396
TaqI TCGA 4 cut(s) 30, 142, 159, 287
TatI WGTACW 1 cut(s) 21
TfiI GAWTC 1 cut(s) 129
Tru1I TTAA 2 cut(s) 354, 390
Tru9I TTAA 2 cut(s) 354, 390
TscAI CASTG 2 cut(s) 79, 273
TseFI GTSAC 1 cut(s) 9
TseI GCWGC 1 cut(s) 274
Tsp45I GTSAC 1 cut(s) 9
TspGWI ACGGA 1 cut(s) 404
TspRI CASTG 2 cut(s) 79, 273
VspI ATTAAT 1 cut(s) 354
XceI RCATGY 1 cut(s) 383
XmiI GTMKAC 1 cut(s) 300
XmnI GAANNNNTTC 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.