RchiOBHm_Chr6g0267791

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0004434
Physical Location & Seq
Forward (+)
24583204 .. 24584503
1300 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ24019

Sequence Viewer

Length: 756 bp
ATGTACCAAGTCGGGGATCCAATTATAGACCACAATTGCTGGGAAAGACCAGAAGACATGGACACAGCCAGGACTGTATATGCCGTGGACTCACCGAATCCAGCTTCTGATGTTGCCGGTGAGACAGCTGCGGCGCTGTCAGCTTCCTCAATGGCATTCCGATCCTATGACCCTGGTTACACAGACACATTGTTGCGAAATGCCATGAAGACCTTCGAGTTTGCTGATACTTACAGAGGAGCTTACAGTGATAATGCCAATGTTAGAGATGGTGTCTGCCCATTTTACTGTGATTTCGATGGTTACCAAGACGAATTGCTATGGAGTGCAGCCTGGCTGAGGAGTGTCACTCAGGATGGTGGTTACCTTGACTACATACAAAACAATGGCAAAACTCTTGGTGCAGAGGACAATATAAACGAATTTGGTTGGGACAACAAGCATGCTGGCCTCAGTGTTCTAATCTCCAAGGAAGTCCTGGAAGGCAACATGTGCAACCTTGAATCCCACAAATCATCGGCTGATAGCTTCATGTGCACATTAATACCGGAATCATCATCCTCCCACATCGAGTACACTCCAGCCGGTCTCATCTACAAGCCAGGAGGCAGCAATCTGCAACATGTAACTTCCATTTCATTCCGGCCTACTACTAGTTTACGCCAATTACCTTGCACGAACCTCGCGATCCCTCAACTGTGGAAACATAGATGTCAGTCCGACAACGCTTCGCCAAATCGCCAAAAAGCAGGTTGA

Protein Analysis

251

Amino Acids

27.75

Weight (kDa)

4.75

Isoelectric Point (pI)

51.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 2 - 214 6.6e-57 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 740
AccII CGCG 1 cut(s) 686
AciI CCGC 1 cut(s) 131
AclWI GGATC 4 cut(s) 11, 24, 156, 682
AcsI RAATTY 1 cut(s) 422
AfaI GTAC 2 cut(s) 5, 575
AfiI CCNNNNNNNGG 2 cut(s) 13, 339
AflIII ACRYGT 2 cut(s) 489, 622
AgsI TTSAA 1 cut(s) 503
AhlI ACTAGT 1 cut(s) 653
AjnI CCWGG 5 cut(s) 68, 172, 332, 477, 601
AluBI AGCT 5 cut(s) 104, 128, 143, 242, 528
AluI AGCT 5 cut(s) 104, 128, 143, 242, 528
Alw21I GWGCWC 1 cut(s) 539
Alw26I GTCTC 2 cut(s) 116, 593
Alw44I GTGCAC 1 cut(s) 535
AlwI GGATC 4 cut(s) 11, 24, 156, 682
AlwNI CAGNNNCTG 1 cut(s) 107
AoxI GGCC 2 cut(s) 448, 644
ApaLI GTGCAC 1 cut(s) 535
ApeKI GCWGC 3 cut(s) 128, 329, 609
ApoI RAATTY 1 cut(s) 422
AseI ATTAAT 1 cut(s) 542
Asp700I GAANNNNTTC 1 cut(s) 212
AspLEI GCGC 1 cut(s) 136
AsuHPI GGTGA 2 cut(s) 84, 131
BaeGI GKGCMC 1 cut(s) 539
BamHI GGATCC 1 cut(s) 16
BbsI GAAGAC 2 cut(s) 60, 215
Bbv12I GWGCWC 1 cut(s) 539
BbvCI CCTCAGC 1 cut(s) 338
BbvI GCAGC 3 cut(s) 115, 341, 621
BccI CCATC 3 cut(s) 263, 293, 350
BceAI ACGGC 1 cut(s) 68
BcgI CGANNNNNNTGC 2 cut(s) 664, 698
BciT130I CCWGG 5 cut(s) 70, 174, 334, 479, 603
BcoDI GTCTC 2 cut(s) 116, 593
BcuI ACTAGT 1 cut(s) 653
BfaI CTAG 1 cut(s) 654
BfoI RGCGCY 1 cut(s) 137
BfuAI ACCTGC 1 cut(s) 740
BisI GCNGC 4 cut(s) 129, 132, 330, 610
BlsI GCNGC 4 cut(s) 130, 133, 331, 611
Bme1390I CCNGG 5 cut(s) 70, 174, 334, 479, 603
BmiI GGNNCC 1 cut(s) 18
BmrFI CCNGG 5 cut(s) 70, 174, 334, 479, 603
BpiI GAAGAC 2 cut(s) 60, 215
BplI GAGNNNNNCTC 2 cut(s) 334, 366
BpmI CTGGAG 1 cut(s) 564
Bpu10I CCTNAGC 1 cut(s) 338
BsaI GGTCTC 1 cut(s) 593
BsaJI CCNNGG 3 cut(s) 84, 172, 468
BsaWI WCCGGW 1 cut(s) 547
Bsc4I CCNNNNNNNGG 2 cut(s) 13, 339
Bse118I RCCGGY 2 cut(s) 116, 584
BseBI CCWGG 5 cut(s) 70, 174, 334, 479, 603
BseDI CCNNGG 3 cut(s) 84, 172, 468
BseGI GGATG 2 cut(s) 361, 557
BseLI CCNNNNNNNGG 2 cut(s) 13, 339
BseMII CTCAG 3 cut(s) 329, 365, 466
BseRI GAGGAG 2 cut(s) 252, 355
BseSI GKGCMC 1 cut(s) 539
BseXI GCAGC 3 cut(s) 115, 341, 621
BseYI CCCAGC 1 cut(s) 39
BsgI GTGCAG 2 cut(s) 348, 423
Bsh1236I CGCG 1 cut(s) 686
BshFI GGCC 2 cut(s) 450, 646
BsiHKAI GWGCWC 1 cut(s) 539
BsiSI CCGG 4 cut(s) 117, 548, 585, 643
BslFI GGGAC 1 cut(s) 446
BslI CCNNNNNNNGG 2 cut(s) 13, 339
BsmAI GTCTC 2 cut(s) 116, 593
BsmFI GGGAC 1 cut(s) 446
BsmI GAATGC 1 cut(s) 155
BsnI GGCC 2 cut(s) 450, 646
Bso31I GGTCTC 1 cut(s) 593
Bsp1286I GDGCHC 1 cut(s) 539
Bsp143I GATC 3 cut(s) 16, 161, 687
Bsp68I TCGCGA 1 cut(s) 686
BspACI CCGC 1 cut(s) 131
BspANI GGCC 2 cut(s) 450, 646
BspCNI CTCAG 3 cut(s) 330, 364, 465
BspFNI CGCG 1 cut(s) 686
BspLI GGNNCC 1 cut(s) 18
BspMI ACCTGC 1 cut(s) 740
BspPI GGATC 4 cut(s) 11, 24, 156, 682
BspTNI GGTCTC 1 cut(s) 593
BsrFI RCCGGY 2 cut(s) 116, 584
BssAI RCCGGY 2 cut(s) 116, 584
BssECI CCNNGG 3 cut(s) 84, 172, 468
BssMI GATC 3 cut(s) 16, 161, 687
BssT1I CCWWGG 1 cut(s) 468
Bst2UI CCWGG 5 cut(s) 70, 174, 334, 479, 603
Bst4CI ACNGT 4 cut(s) 76, 248, 290, 699
BstAPI GCANNNNNTGC 1 cut(s) 492
BstC8I GCNNGC 2 cut(s) 444, 448
BstDEI CTNAG 3 cut(s) 338, 351, 452
BstDSI CCRYGG 1 cut(s) 84
BstEII GGTNACC 2 cut(s) 302, 362
BstENI CCTNNNNNAGG 1 cut(s) 337
BstF5I GGATG 2 cut(s) 361, 557
BstFNI CGCG 1 cut(s) 686
BstH2I RGCGCY 1 cut(s) 137
BstHHI GCGC 1 cut(s) 136
BstKTI GATC 3 cut(s) 19, 164, 690
BstMAI GTCTC 2 cut(s) 116, 593
BstMBI GATC 3 cut(s) 16, 161, 687
BstMWI GCNNNNNNNGC 3 cut(s) 140, 492, 534
BstNI CCWGG 5 cut(s) 70, 174, 334, 479, 603
BstNSI RCATGY 3 cut(s) 446, 493, 626
BstPI GGTNACC 2 cut(s) 302, 362
BstSCI CCNGG 5 cut(s) 68, 172, 332, 477, 601
BstSLI GKGCMC 1 cut(s) 539
BstUI CGCG 1 cut(s) 686
BstV1I GCAGC 3 cut(s) 115, 341, 621
BstV2I GAAGAC 2 cut(s) 60, 215
BstX2I RGATCY 1 cut(s) 16
BstYI RGATCY 1 cut(s) 16
BsuRI GGCC 2 cut(s) 450, 646
BtgI CCRYGG 1 cut(s) 84
BtsCI GGATG 2 cut(s) 361, 557
BtsIMutI CAGTG 2 cut(s) 253, 460
BtuMI TCGCGA 1 cut(s) 686
BveI ACCTGC 1 cut(s) 740
Cac8I GCNNGC 2 cut(s) 444, 448
CaiI CAGNNNCTG 1 cut(s) 107
CfoI GCGC 1 cut(s) 136
Cfr10I RCCGGY 2 cut(s) 116, 584
Csp6I GTAC 2 cut(s) 4, 574
CviAII CATG 6 cut(s) 58, 205, 443, 490, 532, 623
CviQI GTAC 2 cut(s) 4, 574
DdeI CTNAG 3 cut(s) 338, 351, 452
DpnI GATC 3 cut(s) 18, 163, 689
DpnII GATC 3 cut(s) 16, 161, 687
Eco130I CCWWGG 1 cut(s) 468
Eco31I GGTCTC 1 cut(s) 593
Eco91I GGTNACC 2 cut(s) 302, 362
EcoNI CCTNNNNNAGG 1 cut(s) 337
EcoO65I GGTNACC 2 cut(s) 302, 362
EcoRII CCWGG 5 cut(s) 68, 172, 332, 477, 601
EcoT14I CCWWGG 1 cut(s) 468
ErhI CCWWGG 1 cut(s) 468
FaeI CATG 6 cut(s) 61, 208, 446, 493, 535, 626
FaqI GGGAC 1 cut(s) 446
FatI CATG 6 cut(s) 57, 204, 442, 489, 531, 622
Fnu4HI GCNGC 4 cut(s) 129, 132, 330, 610
FokI GGATG 2 cut(s) 368, 544
Fsp4HI GCNGC 4 cut(s) 129, 132, 330, 610
FspBI CTAG 1 cut(s) 654
GlaI GCGC 1 cut(s) 135
GluI GCNGC 4 cut(s) 129, 132, 330, 610
GsaI CCCAGC 1 cut(s) 43
GsuI CTGGAG 1 cut(s) 564
HaeII RGCGCY 1 cut(s) 137
HaeIII GGCC 2 cut(s) 450, 646
HapII CCGG 4 cut(s) 117, 548, 585, 643
HhaI GCGC 1 cut(s) 136
Hin1II CATG 6 cut(s) 61, 208, 446, 493, 535, 626
Hin6I GCGC 1 cut(s) 134
HinP1I GCGC 1 cut(s) 134
HinfI GANTC 4 cut(s) 89, 97, 503, 551
HpaII CCGG 4 cut(s) 117, 548, 585, 643
HphI GGTGA 2 cut(s) 84, 131
Hpy166II GTNNAC 4 cut(s) 88, 537, 576, 659
Hpy188I TCNGA 3 cut(s) 109, 161, 721
Hpy188III TCNNGA 2 cut(s) 353, 685
Hpy8I GTNNAC 4 cut(s) 88, 537, 576, 659
HpyAV CCTTC 2 cut(s) 223, 476
HpyCH4III ACNGT 4 cut(s) 76, 248, 290, 699
HpyCH4V TGCA 6 cut(s) 329, 404, 495, 537, 619, 675
HpyF10VI GCNNNNNNNGC 3 cut(s) 140, 492, 534
HpyF3I CTNAG 3 cut(s) 338, 351, 452
Hsp92II CATG 6 cut(s) 61, 208, 446, 493, 535, 626
HspAI GCGC 1 cut(s) 134
Kzo9I GATC 3 cut(s) 16, 161, 687
LmnI GCTCC 1 cut(s) 239
Lsp1109I GCAGC 3 cut(s) 115, 341, 621
MaeI CTAG 1 cut(s) 654
MaeIII GTNAC 5 cut(s) 176, 302, 346, 362, 625
MalI GATC 3 cut(s) 18, 163, 689
MboI GATC 3 cut(s) 16, 161, 687
MboII GAAGA 2 cut(s) 65, 220
MfeI CAATTG 1 cut(s) 34
MflI RGATCY 1 cut(s) 16
MhlI GDGCHC 1 cut(s) 539
MluCI AATT 5 cut(s) 21, 34, 314, 422, 665
MlyI GAGTC 1 cut(s) 83
MmeI TCCRAC 1 cut(s) 744
MnlI CCTC 9 cut(s) 157, 230, 333, 400, 461, 571, 599, 692, 702
MroXI GAANNNNTTC 1 cut(s) 212
MseI TTAA 1 cut(s) 542
MspA1I CMGCKG 1 cut(s) 128
MspI CCGG 4 cut(s) 117, 548, 585, 643
MspR9I CCNGG 5 cut(s) 70, 174, 334, 479, 603
MunI CAATTG 1 cut(s) 34
Mva1269I GAATGC 1 cut(s) 155
MvaI CCWGG 5 cut(s) 70, 174, 334, 479, 603
MvnI CGCG 1 cut(s) 686
MwoI GCNNNNNNNGC 3 cut(s) 140, 492, 534
NdeII GATC 3 cut(s) 16, 161, 687
NlaIII CATG 6 cut(s) 61, 208, 446, 493, 535, 626
NlaIV GGNNCC 1 cut(s) 18
NmuCI GTSAC 1 cut(s) 346
NruI TCGCGA 1 cut(s) 686
NspI RCATGY 3 cut(s) 446, 493, 626
PaeI GCATGC 1 cut(s) 446
PciI ACATGT 2 cut(s) 489, 622
PcsI WCGNNNNNNNCGW 1 cut(s) 683
PctI GAATGC 1 cut(s) 155
PdmI GAANNNNTTC 1 cut(s) 212
PfeI GAWTC 3 cut(s) 97, 503, 551
PfoI TCCNGGA 1 cut(s) 477
PkrI GCNGC 4 cut(s) 130, 133, 331, 611
PleI GAGTC 1 cut(s) 83
PpsI GAGTC 1 cut(s) 83
PscI ACATGT 2 cut(s) 489, 622
PshBI ATTAAT 1 cut(s) 542
Psp6I CCWGG 5 cut(s) 68, 172, 332, 477, 601
PspEI GGTNACC 2 cut(s) 302, 362
PspFI CCCAGC 1 cut(s) 39
PspGI CCWGG 5 cut(s) 68, 172, 332, 477, 601
PspN4I GGNNCC 1 cut(s) 18
PstNI CAGNNNCTG 1 cut(s) 107
PsuI RGATCY 1 cut(s) 16
PvuII CAGCTG 1 cut(s) 128
RruI TCGCGA 1 cut(s) 686
RsaI GTAC 2 cut(s) 5, 575
RsaNI GTAC 2 cut(s) 4, 574
SaqAI TTAA 1 cut(s) 542
SatI GCNGC 4 cut(s) 129, 132, 330, 610
Sau3AI GATC 3 cut(s) 16, 161, 687
SchI GAGTC 1 cut(s) 83
ScrFI CCNGG 5 cut(s) 70, 174, 334, 479, 603
SduI GDGCHC 1 cut(s) 539
SpeI ACTAGT 1 cut(s) 653
SphI GCATGC 1 cut(s) 446
Sse9I AATT 5 cut(s) 21, 34, 314, 422, 665
SsiI CCGC 1 cut(s) 131
SspMI CTAG 1 cut(s) 654
StyD4I CCNGG 5 cut(s) 68, 172, 332, 477, 601
StyI CCWWGG 1 cut(s) 468
TaaI ACNGT 4 cut(s) 76, 248, 290, 699
TaqI TCGA 3 cut(s) 216, 297, 570
TasI AATT 5 cut(s) 21, 34, 314, 422, 665
TatI WGTACW 1 cut(s) 573
TauI GCSGC 1 cut(s) 134
TfiI GAWTC 3 cut(s) 97, 503, 551
Tru1I TTAA 1 cut(s) 542
Tru9I TTAA 1 cut(s) 542
TscAI CASTG 2 cut(s) 253, 460
TseFI GTSAC 1 cut(s) 346
TseI GCWGC 3 cut(s) 128, 329, 609
Tsp45I GTSAC 1 cut(s) 346
TspDTI ATGAA 3 cut(s) 221, 520, 627
TspRI CASTG 2 cut(s) 253, 460
VneI GTGCAC 1 cut(s) 535
VspI ATTAAT 1 cut(s) 542
XagI CCTNNNNNAGG 1 cut(s) 337
XapI RAATTY 1 cut(s) 422
XceI RCATGY 3 cut(s) 446, 493, 626
XcmI CCANNNNNNNNNTGG 1 cut(s) 475
XmnI GAANNNNTTC 1 cut(s) 212
XspI CTAG 1 cut(s) 654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.