RchiOBHm_Chr6g0267661

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0004434
Physical Location & Seq
Forward (+)
24490069 .. 24491147
1079 bp
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UTR
Exon/CDS
Intron
PRQ24008

Sequence Viewer

Length: 552 bp
ATGGACACAGCTAGGACTGTATATGCCGTGGACTCGCCGAATCCAGCTTCTGATGTTGCTGGTGAGAAAGCTGCGGCGCTGTCAGCTTCCTCAATGGCATTCCGATCCTCTGACCCTGGTTATGCAGACACATTGTTGCGAAATGCCATGAAGGCCTTCGAGTTTGTGGATACTTACAGAGGAGCTTACAATGATAATGCCAATGTTAGAGATGGTGTCTGCCCATTTTACTGTGATTTCGATGGTTACCAAGATGGTGGTTACCTTGACTACATACAAAACAATGGCAAAACTCTTGGTGCAGAGGACAATATAAACGAATTTGGTTGGGACAACAAGCATGCTGGCCTCTATGTTCTAATCTCCAAGGAAGTCCTGGAAGGCAACATGTTCAACCTTGAATCGTACAAATCATCGGCTGATAGCTTCATGTGCACATTAATACCGGAATCATCATCCTCCCACATCGAGTACACTCCAGCCGGTCTCATCTACAAGCCAGGAGGCAGCAATCTGCAACATGTAACTTCGATTTCATTCCTACTATACTAG

Protein Analysis

183

Amino Acids

20.01

Weight (kDa)

4.43

Isoelectric Point (pI)

41.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 1 - 182 5e-33 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 74
AclWI GGATC 1 cut(s) 99
AcsI RAATTY 1 cut(s) 320
AfaI GTAC 2 cut(s) 407, 473
AflIII ACRYGT 2 cut(s) 387, 520
AgsI TTSAA 2 cut(s) 394, 401
AjnI CCWGG 3 cut(s) 115, 375, 499
AluBI AGCT 6 cut(s) 11, 47, 71, 86, 185, 426
AluI AGCT 6 cut(s) 11, 47, 71, 86, 185, 426
Alw21I GWGCWC 1 cut(s) 437
Alw26I GTCTC 1 cut(s) 491
Alw44I GTGCAC 1 cut(s) 433
AlwI GGATC 1 cut(s) 99
AlwNI CAGNNNCTG 1 cut(s) 50
AoxI GGCC 2 cut(s) 153, 346
ApaLI GTGCAC 1 cut(s) 433
ApeKI GCWGC 2 cut(s) 71, 507
ApoI RAATTY 1 cut(s) 320
AseI ATTAAT 1 cut(s) 440
Asp700I GAANNNNTTC 1 cut(s) 155
AspLEI GCGC 1 cut(s) 79
AsuHPI GGTGA 1 cut(s) 74
BaeGI GKGCMC 1 cut(s) 437
Bbv12I GWGCWC 1 cut(s) 437
BbvI GCAGC 2 cut(s) 58, 519
BccI CCATC 3 cut(s) 206, 236, 248
BceAI ACGGC 1 cut(s) 11
BciT130I CCWGG 3 cut(s) 117, 377, 501
BciVI GTATCC 1 cut(s) 163
BcoDI GTCTC 1 cut(s) 491
BfaI CTAG 2 cut(s) 12, 550
BfoI RGCGCY 1 cut(s) 80
BfuI GTATCC 1 cut(s) 163
BglI GCCNNNNNGGC 1 cut(s) 152
BisI GCNGC 3 cut(s) 72, 75, 508
BlsI GCNGC 3 cut(s) 73, 76, 509
Bme1390I CCNGG 3 cut(s) 117, 377, 501
BmrFI CCNGG 3 cut(s) 117, 377, 501
BpmI CTGGAG 1 cut(s) 462
BsaI GGTCTC 1 cut(s) 491
BsaJI CCNNGG 3 cut(s) 27, 115, 366
BsaWI WCCGGW 1 cut(s) 445
Bse118I RCCGGY 1 cut(s) 482
BseBI CCWGG 3 cut(s) 117, 377, 501
BseDI CCNNGG 3 cut(s) 27, 115, 366
BseGI GGATG 1 cut(s) 455
BseRI GAGGAG 1 cut(s) 195
BseSI GKGCMC 1 cut(s) 437
BseXI GCAGC 2 cut(s) 58, 519
BsgI GTGCAG 1 cut(s) 321
BshFI GGCC 2 cut(s) 155, 348
BsiHKAI GWGCWC 1 cut(s) 437
BsiSI CCGG 2 cut(s) 446, 483
BslFI GGGAC 1 cut(s) 344
BsmAI GTCTC 1 cut(s) 491
BsmFI GGGAC 1 cut(s) 344
BsmI GAATGC 1 cut(s) 98
BsnI GGCC 2 cut(s) 155, 348
Bso31I GGTCTC 1 cut(s) 491
Bsp1286I GDGCHC 1 cut(s) 437
Bsp143I GATC 1 cut(s) 104
BspACI CCGC 1 cut(s) 74
BspANI GGCC 2 cut(s) 155, 348
BspPI GGATC 1 cut(s) 99
BspTNI GGTCTC 1 cut(s) 491
BsrFI RCCGGY 1 cut(s) 482
BssAI RCCGGY 1 cut(s) 482
BssECI CCNNGG 3 cut(s) 27, 115, 366
BssMI GATC 1 cut(s) 104
BssT1I CCWWGG 1 cut(s) 366
Bst2UI CCWGG 3 cut(s) 117, 377, 501
Bst4CI ACNGT 2 cut(s) 19, 233
BstC8I GCNNGC 2 cut(s) 342, 346
BstDSI CCRYGG 1 cut(s) 27
BstEII GGTNACC 2 cut(s) 245, 260
BstF5I GGATG 1 cut(s) 455
BstH2I RGCGCY 1 cut(s) 80
BstHHI GCGC 1 cut(s) 79
BstKTI GATC 1 cut(s) 107
BstMAI GTCTC 1 cut(s) 491
BstMBI GATC 1 cut(s) 104
BstMWI GCNNNNNNNGC 3 cut(s) 83, 152, 432
BstNI CCWGG 3 cut(s) 117, 377, 501
BstNSI RCATGY 3 cut(s) 344, 391, 524
BstPI GGTNACC 2 cut(s) 245, 260
BstSCI CCNGG 3 cut(s) 115, 375, 499
BstSLI GKGCMC 1 cut(s) 437
BstV1I GCAGC 2 cut(s) 58, 519
BstXI CCANNNNNNTGG 1 cut(s) 257
BsuI GTATCC 1 cut(s) 163
BsuRI GGCC 2 cut(s) 155, 348
BtgI CCRYGG 1 cut(s) 27
BtsCI GGATG 1 cut(s) 455
Cac8I GCNNGC 2 cut(s) 342, 346
CaiI CAGNNNCTG 1 cut(s) 50
CfoI GCGC 1 cut(s) 79
Cfr10I RCCGGY 1 cut(s) 482
Csp6I GTAC 2 cut(s) 406, 472
CviAII CATG 5 cut(s) 148, 341, 388, 430, 521
CviQI GTAC 2 cut(s) 406, 472
DpnI GATC 1 cut(s) 106
DpnII GATC 1 cut(s) 104
Eco130I CCWWGG 1 cut(s) 366
Eco147I AGGCCT 1 cut(s) 155
Eco31I GGTCTC 1 cut(s) 491
Eco91I GGTNACC 2 cut(s) 245, 260
EcoO65I GGTNACC 2 cut(s) 245, 260
EcoRII CCWGG 3 cut(s) 115, 375, 499
EcoT14I CCWWGG 1 cut(s) 366
ErhI CCWWGG 1 cut(s) 366
FaeI CATG 5 cut(s) 151, 344, 391, 433, 524
FaqI GGGAC 1 cut(s) 344
FatI CATG 5 cut(s) 147, 340, 387, 429, 520
Fnu4HI GCNGC 3 cut(s) 72, 75, 508
FokI GGATG 1 cut(s) 442
Fsp4HI GCNGC 3 cut(s) 72, 75, 508
FspBI CTAG 2 cut(s) 12, 550
GlaI GCGC 1 cut(s) 78
GluI GCNGC 3 cut(s) 72, 75, 508
GsuI CTGGAG 1 cut(s) 462
HaeII RGCGCY 1 cut(s) 80
HaeIII GGCC 2 cut(s) 155, 348
HapII CCGG 2 cut(s) 446, 483
HhaI GCGC 1 cut(s) 79
Hin1II CATG 5 cut(s) 151, 344, 391, 433, 524
Hin6I GCGC 1 cut(s) 77
HinP1I GCGC 1 cut(s) 77
HinfI GANTC 4 cut(s) 32, 40, 401, 449
HpaII CCGG 2 cut(s) 446, 483
HphI GGTGA 1 cut(s) 74
Hpy166II GTNNAC 3 cut(s) 31, 435, 474
Hpy188I TCNGA 3 cut(s) 52, 104, 112
Hpy8I GTNNAC 3 cut(s) 31, 435, 474
HpyAV CCTTC 3 cut(s) 145, 166, 374
HpyCH4III ACNGT 2 cut(s) 19, 233
HpyCH4V TGCA 4 cut(s) 125, 302, 435, 517
HpyF10VI GCNNNNNNNGC 3 cut(s) 83, 152, 432
Hsp92II CATG 5 cut(s) 151, 344, 391, 433, 524
HspAI GCGC 1 cut(s) 77
Kzo9I GATC 1 cut(s) 104
LmnI GCTCC 1 cut(s) 182
Lsp1109I GCAGC 2 cut(s) 58, 519
MaeI CTAG 2 cut(s) 12, 550
MaeIII GTNAC 3 cut(s) 245, 260, 523
MalI GATC 1 cut(s) 106
MboI GATC 1 cut(s) 104
MhlI GDGCHC 1 cut(s) 437
MluCI AATT 1 cut(s) 320
MlyI GAGTC 1 cut(s) 26
MnlI CCTC 7 cut(s) 100, 118, 173, 298, 359, 469, 497
MroXI GAANNNNTTC 1 cut(s) 155
MseI TTAA 1 cut(s) 440
MspI CCGG 2 cut(s) 446, 483
MspR9I CCNGG 3 cut(s) 117, 377, 501
Mva1269I GAATGC 1 cut(s) 98
MvaI CCWGG 3 cut(s) 117, 377, 501
MwoI GCNNNNNNNGC 3 cut(s) 83, 152, 432
NdeII GATC 1 cut(s) 104
NlaIII CATG 5 cut(s) 151, 344, 391, 433, 524
NspI RCATGY 3 cut(s) 344, 391, 524
PaeI GCATGC 1 cut(s) 344
PceI AGGCCT 1 cut(s) 155
PciI ACATGT 2 cut(s) 387, 520
PctI GAATGC 1 cut(s) 98
PdmI GAANNNNTTC 1 cut(s) 155
PfeI GAWTC 3 cut(s) 40, 401, 449
PfoI TCCNGGA 1 cut(s) 375
PkrI GCNGC 3 cut(s) 73, 76, 509
PleI GAGTC 1 cut(s) 26
PpsI GAGTC 1 cut(s) 26
PscI ACATGT 2 cut(s) 387, 520
PshBI ATTAAT 1 cut(s) 440
Psp6I CCWGG 3 cut(s) 115, 375, 499
PspEI GGTNACC 2 cut(s) 245, 260
PspGI CCWGG 3 cut(s) 115, 375, 499
PstNI CAGNNNCTG 1 cut(s) 50
RsaI GTAC 2 cut(s) 407, 473
RsaNI GTAC 2 cut(s) 406, 472
SaqAI TTAA 1 cut(s) 440
SatI GCNGC 3 cut(s) 72, 75, 508
Sau3AI GATC 1 cut(s) 104
SchI GAGTC 1 cut(s) 26
ScrFI CCNGG 3 cut(s) 117, 377, 501
SduI GDGCHC 1 cut(s) 437
SetI ASST 8 cut(s) 13, 49, 73, 88, 187, 267, 399, 428
SphI GCATGC 1 cut(s) 344
Sse9I AATT 1 cut(s) 320
SseBI AGGCCT 1 cut(s) 155
SsiI CCGC 1 cut(s) 74
SspMI CTAG 2 cut(s) 12, 550
StuI AGGCCT 1 cut(s) 155
StyD4I CCNGG 3 cut(s) 115, 375, 499
StyI CCWWGG 1 cut(s) 366
TaaI ACNGT 2 cut(s) 19, 233
TaqI TCGA 4 cut(s) 159, 240, 468, 530
TasI AATT 1 cut(s) 320
TatI WGTACW 1 cut(s) 471
TauI GCSGC 1 cut(s) 77
TfiI GAWTC 3 cut(s) 40, 401, 449
Tru1I TTAA 1 cut(s) 440
Tru9I TTAA 1 cut(s) 440
TseI GCWGC 2 cut(s) 71, 507
TspDTI ATGAA 3 cut(s) 164, 418, 525
VneI GTGCAC 1 cut(s) 433
VspI ATTAAT 1 cut(s) 440
XapI RAATTY 1 cut(s) 320
XceI RCATGY 3 cut(s) 344, 391, 524
XcmI CCANNNNNNNNNTGG 1 cut(s) 373
XmnI GAANNNNTTC 1 cut(s) 155
XspI CTAG 2 cut(s) 12, 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.