RchiOBHm_Chr6g0267801

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0004434
Physical Location & Seq
Forward (+)
24584505 .. 24584827
323 bp
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UTR
Exon/CDS
Intron
PRQ24020

Sequence Viewer

Length: 306 bp
ATGGGTTTGTCATACATGGTCGGATACAGCGACCATTATCCACAGCGAATTCACCACCGTGGATCTTCTTTACCATCTATCAAGGATCATCCCCAACCCATTGCTTGCAAGGAAGGCTCAGTGTACTTCAATTCGTCAAGCCCTAACCTTAATCCCCTTGTGGGAGCCCTAGTAGGAGGACCTGGAGAAGATGATGTCTATGAGGATGATCGCGCTTATTTTAGGAAGTCTGAACCGACCACATACATTAATGCACCATTTGTTGGTGTTTTGGCATACTTTGTGGCTAATCCCAGTCCAAACTAG

Protein Analysis

101

Amino Acids

11.08

Weight (kDa)

5.67

Isoelectric Point (pI)

52.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 1 - 91 6.2e-26 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 263
AccII CGCG 1 cut(s) 213
AclWI GGATC 2 cut(s) 70, 93
AcsI RAATTY 1 cut(s) 48
AfaI GTAC 1 cut(s) 125
AfiI CCNNNNNNNGG 2 cut(s) 161, 263
AgsI TTSAA 1 cut(s) 130
AjnI CCWGG 1 cut(s) 181
AleI CACNNNNGTG 1 cut(s) 57
AlwI GGATC 2 cut(s) 70, 93
ApoI RAATTY 1 cut(s) 48
AseI ATTAAT 1 cut(s) 249
AspLEI GCGC 1 cut(s) 215
AspS9I GGNCC 1 cut(s) 179
AsuHPI GGTGA 1 cut(s) 44
AvaII GGWCC 1 cut(s) 179
BanII GRGCYC 1 cut(s) 169
BccI CCATC 1 cut(s) 82
BciT130I CCWGG 1 cut(s) 183
BciVI GTATCC 1 cut(s) 17
BfaI CTAG 2 cut(s) 170, 304
BfuI GTATCC 1 cut(s) 17
Bme1390I CCNGG 1 cut(s) 183
Bme18I GGWCC 1 cut(s) 179
BmgT120I GGNCC 1 cut(s) 179
BmiI GGNNCC 1 cut(s) 166
BmrFI CCNGG 1 cut(s) 183
BmrI ACTGGG 1 cut(s) 288
BmuI ACTGGG 1 cut(s) 288
BpmI CTGGAG 1 cut(s) 204
BsaJI CCNNGG 1 cut(s) 58
BsaXI ACNNNNNCTCC 2 cut(s) 156, 186
Bsc4I CCNNNNNNNGG 2 cut(s) 161, 263
Bse1I ACTGG 1 cut(s) 294
Bse3DI GCAATG 1 cut(s) 99
BseBI CCWGG 1 cut(s) 183
BseDI CCNNGG 1 cut(s) 58
BseGI GGATG 2 cut(s) 88, 211
BseLI CCNNNNNNNGG 2 cut(s) 161, 263
BseMI GCAATG 1 cut(s) 99
BseMII CTCAG 1 cut(s) 132
BseNI ACTGG 1 cut(s) 294
Bsh1236I CGCG 1 cut(s) 213
BslI CCNNNNNNNGG 2 cut(s) 161, 263
Bsp1286I GDGCHC 1 cut(s) 169
Bsp143I GATC 3 cut(s) 62, 85, 208
BspCNI CTCAG 1 cut(s) 131
BspFNI CGCG 1 cut(s) 213
BspLI GGNNCC 1 cut(s) 166
BspPI GGATC 2 cut(s) 70, 93
BsrDI GCAATG 1 cut(s) 99
BsrI ACTGG 1 cut(s) 294
BssECI CCNNGG 1 cut(s) 58
BssMI GATC 3 cut(s) 62, 85, 208
Bst2UI CCWGG 1 cut(s) 183
Bst4CI ACNGT 1 cut(s) 59
BstC8I GCNNGC 1 cut(s) 106
BstDEI CTNAG 1 cut(s) 118
BstDSI CCRYGG 1 cut(s) 58
BstF5I GGATG 2 cut(s) 88, 211
BstFNI CGCG 1 cut(s) 213
BstHHI GCGC 1 cut(s) 215
BstKTI GATC 3 cut(s) 65, 88, 211
BstMBI GATC 3 cut(s) 62, 85, 208
BstMWI GCNNNNNNNGC 1 cut(s) 114
BstNI CCWGG 1 cut(s) 183
BstSCI CCNGG 1 cut(s) 181
BstUI CGCG 1 cut(s) 213
BstX2I RGATCY 1 cut(s) 62
BstYI RGATCY 1 cut(s) 62
BsuI GTATCC 1 cut(s) 17
BtgI CCRYGG 1 cut(s) 58
BtsCI GGATG 2 cut(s) 88, 211
BtsIMutI CAGTG 1 cut(s) 126
Cac8I GCNNGC 1 cut(s) 106
CfoI GCGC 1 cut(s) 215
Cfr13I GGNCC 1 cut(s) 179
Csp6I GTAC 1 cut(s) 124
CviAII CATG 1 cut(s) 16
CviJI RGCY 4 cut(s) 117, 141, 167, 287
CviKI_1 RGCY 4 cut(s) 117, 141, 167, 287
CviQI GTAC 1 cut(s) 124
DdeI CTNAG 1 cut(s) 118
DpnI GATC 3 cut(s) 64, 87, 210
DpnII GATC 3 cut(s) 62, 85, 208
Eco24I GRGCYC 1 cut(s) 169
Eco47I GGWCC 1 cut(s) 179
EcoO109I RGGNCCY 1 cut(s) 179
EcoRI GAATTC 1 cut(s) 48
EcoRII CCWGG 1 cut(s) 181
EcoT38I GRGCYC 1 cut(s) 169
FaeI CATG 1 cut(s) 19
FaiI YATR 5 cut(s) 13, 17, 201, 244, 277
FatI CATG 1 cut(s) 15
FokI GGATG 2 cut(s) 75, 218
FriOI GRGCYC 1 cut(s) 169
FspBI CTAG 2 cut(s) 170, 304
GlaI GCGC 1 cut(s) 214
GsuI CTGGAG 1 cut(s) 204
HhaI GCGC 1 cut(s) 215
Hin1II CATG 1 cut(s) 19
Hin6I GCGC 1 cut(s) 213
HinP1I GCGC 1 cut(s) 213
HphI GGTGA 1 cut(s) 44
Hpy166II GTNNAC 1 cut(s) 124
Hpy188I TCNGA 2 cut(s) 23, 232
Hpy8I GTNNAC 1 cut(s) 124
HpyAV CCTTC 1 cut(s) 107
HpyCH4III ACNGT 1 cut(s) 59
HpyCH4V TGCA 2 cut(s) 108, 254
HpyF10VI GCNNNNNNNGC 1 cut(s) 114
HpyF3I CTNAG 1 cut(s) 118
Hsp92II CATG 1 cut(s) 19
HspAI GCGC 1 cut(s) 213
Kzo9I GATC 3 cut(s) 62, 85, 208
LmnI GCTCC 1 cut(s) 164
LpnPI CCDG 2 cut(s) 168, 195
MaeI CTAG 2 cut(s) 170, 304
MalI GATC 3 cut(s) 64, 87, 210
MboI GATC 3 cut(s) 62, 85, 208
MboII GAAGA 2 cut(s) 57, 200
MflI RGATCY 1 cut(s) 62
MhlI GDGCHC 1 cut(s) 169
MluCI AATT 2 cut(s) 48, 130
MnlI CCTC 2 cut(s) 170, 196
MseI TTAA 2 cut(s) 150, 249
MslI CAYNNNNRTG 1 cut(s) 57
MspR9I CCNGG 1 cut(s) 183
MvaI CCWGG 1 cut(s) 183
MvnI CGCG 1 cut(s) 213
MwoI GCNNNNNNNGC 1 cut(s) 114
NdeII GATC 3 cut(s) 62, 85, 208
NlaIII CATG 1 cut(s) 19
NlaIV GGNNCC 1 cut(s) 166
OliI CACNNNNGTG 1 cut(s) 57
PcsI WCGNNNNNNNCGW 1 cut(s) 27
PflMI CCANNNNNTGG 1 cut(s) 263
PpuMI RGGWCCY 1 cut(s) 179
PshBI ATTAAT 1 cut(s) 249
Psp5II RGGWCCY 1 cut(s) 179
Psp6I CCWGG 1 cut(s) 181
PspGI CCWGG 1 cut(s) 181
PspN4I GGNNCC 1 cut(s) 166
PspPI GGNCC 1 cut(s) 179
PspPPI RGGWCCY 1 cut(s) 179
PsuI RGATCY 1 cut(s) 62
RsaI GTAC 1 cut(s) 125
RsaNI GTAC 1 cut(s) 124
RseI CAYNNNNRTG 1 cut(s) 57
SaqAI TTAA 2 cut(s) 150, 249
Sau3AI GATC 3 cut(s) 62, 85, 208
Sau96I GGNCC 1 cut(s) 179
ScrFI CCNGG 1 cut(s) 183
SduI GDGCHC 1 cut(s) 169
SetI ASST 2 cut(s) 150, 184
SinI GGWCC 1 cut(s) 179
SmiMI CAYNNNNRTG 1 cut(s) 57
Sse9I AATT 2 cut(s) 48, 130
SspMI CTAG 2 cut(s) 170, 304
StyD4I CCNGG 1 cut(s) 181
TaaI ACNGT 1 cut(s) 59
TasI AATT 2 cut(s) 48, 130
TatI WGTACW 1 cut(s) 123
Tru1I TTAA 2 cut(s) 150, 249
Tru9I TTAA 2 cut(s) 150, 249
TscAI CASTG 1 cut(s) 126
TspRI CASTG 1 cut(s) 126
Van91I CCANNNNNTGG 1 cut(s) 263
VpaK11BI GGWCC 1 cut(s) 179
VspI ATTAAT 1 cut(s) 249
XapI RAATTY 1 cut(s) 48
XspI CTAG 2 cut(s) 170, 304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.