RchiOBHm_Chr6g0267611

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0004434
Physical Location & Seq
Forward (+)
24454458 .. 24457907
3450 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ24004

Sequence Viewer

Length: 1485 bp
ATGAAACCCATGACTCATTTCCTCTCCCTCCTTCTTCTCCTAATCTGCCTCCTCGCCACCGTGCACGGCGGAGGGCACGACTACCAGGACGCATTGTCGAAATGCATACTCTTTTTTGAGGGCCAGAGGTCGGGGGTTCTGCCTCAGGACCAACGCCAGTCGTGGCGGGCTAATTCCGGGTTGAGTGATGGCTGGACTTACAACACTGACCTCACCGGCGGCTACTACGACGCCGGAGACAACGTCAAGTTCGGGTTCCCGATGGCCTTCACGACCACAATGCTGGCCTGGAGTGTCATCGAGTTTGGGGACTCCATGCCTCCCAATGAGCACAGGAATGCGCTGGTTGCCCTGCGTTGGGGGACGGATTACCTCCTCAAAACGGTGTCGCAGCCCAATCGGATTTTCGTACAAGTCGGGGATCCAATTATAGACCACAATTGCTGGGAAAGACCAGAAGACATGGACACAGCCAGGACTGTATATGCCGTGGACTCGCCGAATCCAGCTTCTGATGTTGCCGGTGAGACAGCTGCGGCGCTGTCAGCTTCCTCAATGGCATTCCGATCCTCTGACCCTAGTTACGCAGACACATTGTTGCGAAATGCCATGAAGGCCTTCGAGTTTGCTGATACTTACAGAGGAGCTTACAGTGATAATGCCAATGTTAGAGATGGTGTCTGCCCATTTTACTGTGATTTCGATGGTTACCAAGACGAATTGCTATGGAGTGCAGCCTGGCTGAGGAGAGTCACTCAGGATGGTGGTTACCTTGACTACATACAAAACAATGGCAAAACTCTTGGTGCAGAGGACAATATAAACGAATTTGGCTGGGACAACAAGCATGCTGGCCTCAATGTTCTAATCTCGAAGGAAGTCCTGGAAGGCAACATGTACAACCTTGAATCGTACAAATCATCGGCTGATAGCTTCATGTGCACATTAATACCGGAGTCGTCATCCTCCCACATCGAGTACACTCCAGCCGGTCTCATCTACAAGCCAGGAGGCAGCAATCTGCAACATGTAACTTCCATTTCATTCCTACTACTAGTTTACGCCAATTACCTTGCACGAACCTCGCGATCCCTCAACTGTGGAAACATAGATGTCAGTCCAACAACGCTTCGCCAAATCGCCAAAAAGCAGGTTGATTACATTTTGGGTGATAATCCAATGGGTTTGTCATACATGGTCGGATACAGCGACCATTATCCGCAGCGAATTCACCATCGTGGATCTTCTTTACCATCTATCAAGGATCATCCCCAACCCATTGCTTGCAAGGAAGGCTCAGTGTACTTCAATTCGTCAAACCCCAACCCTAATCCCCTTGTGGGAGCCCTAGTAGGAGGACCTGGAGAAGATGATGTCTATGAGGATGATCGCGCTGATTTTAGGAAGTCCGAACCGACCACATACATTAATGCACCATTTGTTGGTGTTTTGGCATACTTTGTGGCTAATCCCAGTCCAAACTAG

Protein Analysis

494

Amino Acids

54.62

Weight (kDa)

4.89

Isoelectric Point (pI)

47.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 28 - 484 4.7e-157 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1141
AccB7I CCANNNNNTGG 1 cut(s) 1442
AccII CGCG 2 cut(s) 1087, 1392
AciI CCGC 5 cut(s) 69, 166, 219, 536, 1220
AclWI GGATC 6 cut(s) 416, 429, 561, 1083, 1249, 1272
AcsI RAATTY 2 cut(s) 827, 1227
AcyI GRCGYC 1 cut(s) 231
AfaI GTAC 5 cut(s) 411, 899, 914, 980, 1304
AfiI CCNNNNNNNGG 7 cut(s) 130, 357, 358, 382, 744, 1340, 1442
AflIII ACRYGT 2 cut(s) 894, 1027
AgsI TTSAA 2 cut(s) 908, 1309
AhdI GACNNNNNGTC 1 cut(s) 94
AhlI ACTAGT 1 cut(s) 1054
AjnI CCWGG 7 cut(s) 84, 287, 473, 737, 882, 1006, 1360
AjuI GAANNNNNNNTTGG 2 cut(s) 389, 421
AleI CACNNNNGTG 1 cut(s) 1236
AluBI AGCT 5 cut(s) 509, 533, 548, 647, 933
AluI AGCT 5 cut(s) 509, 533, 548, 647, 933
Alw21I GWGCWC 3 cut(s) 66, 333, 944
Alw26I GTCTC 3 cut(s) 231, 521, 998
Alw44I GTGCAC 2 cut(s) 62, 940
AlwI GGATC 6 cut(s) 416, 429, 561, 1083, 1249, 1272
AlwNI CAGNNNCTG 1 cut(s) 512
AoxI GGCC 5 cut(s) 121, 264, 285, 615, 853
ApaLI GTGCAC 2 cut(s) 62, 940
ApeKI GCWGC 5 cut(s) 391, 533, 734, 1014, 1222
ApoI RAATTY 2 cut(s) 827, 1227
AseI ATTAAT 2 cut(s) 947, 1428
Asp700I GAANNNNTTC 1 cut(s) 617
AspLEI GCGC 3 cut(s) 343, 541, 1394
AspS9I GGNCC 3 cut(s) 121, 148, 1358
AsuC2I CCSGG 1 cut(s) 178
AsuHPI GGTGA 4 cut(s) 205, 536, 1181, 1223
AvaII GGWCC 2 cut(s) 148, 1358
AxyI CCTNAGG 1 cut(s) 144
BaeGI GKGCMC 3 cut(s) 66, 78, 944
BamHI GGATCC 1 cut(s) 421
BanII GRGCYC 1 cut(s) 1348
BbsI GAAGAC 1 cut(s) 465
Bbv12I GWGCWC 3 cut(s) 66, 333, 944
BbvCI CCTCAGC 1 cut(s) 743
BbvI GCAGC 5 cut(s) 403, 520, 746, 1026, 1234
BccI CCATC 7 cut(s) 182, 256, 668, 698, 755, 1242, 1261
BceAI ACGGC 2 cut(s) 82, 473
BcgI CGANNNNNNTGC 6 cut(s) 262, 296, 380, 414, 1065, 1099
BciT130I CCWGG 7 cut(s) 86, 289, 475, 739, 884, 1008, 1362
BciVI GTATCC 1 cut(s) 1196
BcnI CCSGG 1 cut(s) 178
BcoDI GTCTC 3 cut(s) 231, 521, 998
BcuI ACTAGT 1 cut(s) 1054
BfaI CTAG 4 cut(s) 579, 1055, 1349, 1483
BfoI RGCGCY 1 cut(s) 542
BfuAI ACCTGC 1 cut(s) 1141
BfuI GTATCC 1 cut(s) 1196
BglI GCCNNNNNGGC 1 cut(s) 614
BisI GCNGC 7 cut(s) 220, 392, 534, 537, 735, 1015, 1223
BlsI GCNGC 7 cut(s) 221, 393, 535, 538, 736, 1016, 1224
Bme1390I CCNGG 8 cut(s) 86, 178, 289, 475, 739, 884, 1008, 1362
Bme18I GGWCC 2 cut(s) 148, 1358
BmeRI GACNNNNNGTC 1 cut(s) 94
BmgT120I GGNCC 3 cut(s) 121, 148, 1358
BmiI GGNNCC 3 cut(s) 257, 423, 1345
BmrFI CCNGG 8 cut(s) 86, 178, 289, 475, 739, 884, 1008, 1362
BmrI ACTGGG 1 cut(s) 1467
BmuI ACTGGG 1 cut(s) 1467
BpiI GAAGAC 1 cut(s) 465
BplI GAGNNNNNCTC 2 cut(s) 739, 771
BpmI CTGGAG 3 cut(s) 310, 969, 1383
Bpu10I CCTNAGC 1 cut(s) 743
BpuMI CCSGG 1 cut(s) 178
BsaHI GRCGYC 1 cut(s) 231
BsaI GGTCTC 1 cut(s) 998
BsaJI CCNNGG 1 cut(s) 489
BsaWI WCCGGW 1 cut(s) 952
BsaXI ACNNNNNCTCC 4 cut(s) 228, 258, 1335, 1365
Bsc4I CCNNNNNNNGG 7 cut(s) 130, 357, 358, 382, 744, 1340, 1442
Bse118I RCCGGY 3 cut(s) 215, 521, 989
Bse1I ACTGG 2 cut(s) 157, 1473
Bse21I CCTNAGG 1 cut(s) 144
Bse3DI GCAATG 1 cut(s) 1278
BseBI CCWGG 7 cut(s) 86, 289, 475, 739, 884, 1008, 1362
BseDI CCNNGG 1 cut(s) 489
BseGI GGATG 4 cut(s) 766, 962, 1267, 1390
BseLI CCNNNNNNNGG 7 cut(s) 130, 357, 358, 382, 744, 1340, 1442
BseMI GCAATG 1 cut(s) 1278
BseMII CTCAG 4 cut(s) 158, 734, 770, 1311
BseNI ACTGG 2 cut(s) 157, 1473
BseRI GAGGAG 4 cut(s) 41, 365, 657, 760
BseSI GKGCMC 3 cut(s) 66, 78, 944
BseXI GCAGC 5 cut(s) 403, 520, 746, 1026, 1234
BseYI CCCAGC 2 cut(s) 444, 834
BsgI GTGCAG 2 cut(s) 753, 828
Bsh1236I CGCG 2 cut(s) 1087, 1392
BshFI GGCC 5 cut(s) 123, 266, 287, 617, 855
BsiHKAI GWGCWC 3 cut(s) 66, 333, 944
BsiSI CCGG 6 cut(s) 177, 216, 234, 522, 953, 990
BslFI GGGAC 3 cut(s) 323, 376, 851
BslI CCNNNNNNNGG 7 cut(s) 130, 357, 358, 382, 744, 1340, 1442
BsmAI GTCTC 3 cut(s) 231, 521, 998
BsmFI GGGAC 3 cut(s) 323, 376, 851
BsmI GAATGC 2 cut(s) 343, 560
BsnI GGCC 5 cut(s) 123, 266, 287, 617, 855
Bso31I GGTCTC 1 cut(s) 998
Bsp1286I GDGCHC 5 cut(s) 66, 78, 333, 944, 1348
Bsp1407I TGTACA 1 cut(s) 897
Bsp143I GATC 6 cut(s) 421, 566, 1088, 1241, 1264, 1387
Bsp68I TCGCGA 1 cut(s) 1087
BspACI CCGC 5 cut(s) 69, 166, 219, 536, 1220
BspANI GGCC 5 cut(s) 123, 266, 287, 617, 855
BspCNI CTCAG 4 cut(s) 157, 735, 769, 1310
BspFNI CGCG 2 cut(s) 1087, 1392
BspLI GGNNCC 3 cut(s) 257, 423, 1345
BspMI ACCTGC 1 cut(s) 1141
BspPI GGATC 6 cut(s) 416, 429, 561, 1083, 1249, 1272
BspTNI GGTCTC 1 cut(s) 998
BsrDI GCAATG 1 cut(s) 1278
BsrFI RCCGGY 3 cut(s) 215, 521, 989
BsrGI TGTACA 1 cut(s) 897
BsrI ACTGG 2 cut(s) 157, 1473
BssAI RCCGGY 3 cut(s) 215, 521, 989
BssECI CCNNGG 1 cut(s) 489
BssMI GATC 6 cut(s) 421, 566, 1088, 1241, 1264, 1387
BssNI GRCGYC 1 cut(s) 231
Bst2UI CCWGG 7 cut(s) 86, 289, 475, 739, 884, 1008, 1362
Bst4CI ACNGT 6 cut(s) 61, 385, 481, 653, 695, 1100
BstACI GRCGYC 1 cut(s) 231
BstAUI TGTACA 1 cut(s) 897
BstC8I GCNNGC 5 cut(s) 168, 285, 849, 853, 1285
BstDEI CTNAG 4 cut(s) 144, 743, 756, 1297
BstDSI CCRYGG 1 cut(s) 489
BstEII GGTNACC 2 cut(s) 707, 767
BstENI CCTNNNNNAGG 1 cut(s) 742
BstF5I GGATG 4 cut(s) 766, 962, 1267, 1390
BstFNI CGCG 2 cut(s) 1087, 1392
BstH2I RGCGCY 1 cut(s) 542
BstHHI GCGC 3 cut(s) 343, 541, 1394
BstKTI GATC 6 cut(s) 424, 569, 1091, 1244, 1267, 1390
BstMAI GTCTC 3 cut(s) 231, 521, 998
BstMBI GATC 6 cut(s) 421, 566, 1088, 1241, 1264, 1387
BstMWI GCNNNNNNNGC 5 cut(s) 347, 545, 614, 939, 1293
BstNI CCWGG 7 cut(s) 86, 289, 475, 739, 884, 1008, 1362
BstNSI RCATGY 3 cut(s) 851, 898, 1031
BstPI GGTNACC 2 cut(s) 707, 767
BstSCI CCNGG 8 cut(s) 84, 176, 287, 473, 737, 882, 1006, 1360
BstSLI GKGCMC 3 cut(s) 66, 78, 944
BstUI CGCG 2 cut(s) 1087, 1392
BstV1I GCAGC 5 cut(s) 403, 520, 746, 1026, 1234
BstV2I GAAGAC 1 cut(s) 465
BstX2I RGATCY 2 cut(s) 421, 1241
BstXI CCANNNNNNTGG 1 cut(s) 283
BstYI RGATCY 2 cut(s) 421, 1241
Bsu36I CCTNAGG 1 cut(s) 144
BsuI GTATCC 1 cut(s) 1196
BsuRI GGCC 5 cut(s) 123, 266, 287, 617, 855
BtgI CCRYGG 1 cut(s) 489
BtsCI GGATG 4 cut(s) 766, 962, 1267, 1390
BtsIMutI CAGTG 3 cut(s) 204, 658, 1305
BtuMI TCGCGA 1 cut(s) 1087
BveI ACCTGC 1 cut(s) 1141
Cac8I GCNNGC 5 cut(s) 168, 285, 849, 853, 1285
CaiI CAGNNNCTG 1 cut(s) 512
CfoI GCGC 3 cut(s) 343, 541, 1394
Cfr10I RCCGGY 3 cut(s) 215, 521, 989
Cfr13I GGNCC 3 cut(s) 121, 148, 1358
CseI GACGC 2 cut(s) 98, 239
Csp6I GTAC 5 cut(s) 410, 898, 913, 979, 1303
CviAII CATG 9 cut(s) 10, 316, 463, 610, 848, 895, 937, 1028, 1195
CviQI GTAC 5 cut(s) 410, 898, 913, 979, 1303
DdeI CTNAG 4 cut(s) 144, 743, 756, 1297
DpnI GATC 6 cut(s) 423, 568, 1090, 1243, 1266, 1389
DpnII GATC 6 cut(s) 421, 566, 1088, 1241, 1264, 1387
DriI GACNNNNNGTC 1 cut(s) 94
Eam1105I GACNNNNNGTC 1 cut(s) 94
EciI GGCGGA 1 cut(s) 84
Eco147I AGGCCT 1 cut(s) 617
Eco24I GRGCYC 1 cut(s) 1348
Eco31I GGTCTC 1 cut(s) 998
Eco47I GGWCC 2 cut(s) 148, 1358
Eco81I CCTNAGG 1 cut(s) 144
Eco91I GGTNACC 2 cut(s) 707, 767
EcoNI CCTNNNNNAGG 1 cut(s) 742
EcoO109I RGGNCCY 1 cut(s) 1358
EcoO65I GGTNACC 2 cut(s) 707, 767
EcoRI GAATTC 1 cut(s) 1227
EcoRII CCWGG 7 cut(s) 84, 287, 473, 737, 882, 1006, 1360
EcoT22I ATGCAT 1 cut(s) 107
EcoT38I GRGCYC 1 cut(s) 1348
FaeI CATG 9 cut(s) 13, 319, 466, 613, 851, 898, 940, 1031, 1198
FaqI GGGAC 3 cut(s) 323, 376, 851
FatI CATG 9 cut(s) 9, 315, 462, 609, 847, 894, 936, 1027, 1194
FauI CCCGC 1 cut(s) 159
Fnu4HI GCNGC 7 cut(s) 220, 392, 534, 537, 735, 1015, 1223
FokI GGATG 4 cut(s) 773, 949, 1254, 1397
FriOI GRGCYC 1 cut(s) 1348
Fsp4HI GCNGC 7 cut(s) 220, 392, 534, 537, 735, 1015, 1223
FspBI CTAG 4 cut(s) 579, 1055, 1349, 1483
GlaI GCGC 3 cut(s) 342, 540, 1393
GluI GCNGC 7 cut(s) 220, 392, 534, 537, 735, 1015, 1223
GsaI CCCAGC 2 cut(s) 448, 838
GsuI CTGGAG 3 cut(s) 310, 969, 1383
HaeII RGCGCY 1 cut(s) 542
HaeIII GGCC 5 cut(s) 123, 266, 287, 617, 855
HapII CCGG 6 cut(s) 177, 216, 234, 522, 953, 990
HgaI GACGC 2 cut(s) 98, 239
HhaI GCGC 3 cut(s) 343, 541, 1394
Hin1I GRCGYC 1 cut(s) 231
Hin1II CATG 9 cut(s) 13, 319, 466, 613, 851, 898, 940, 1031, 1198
Hin6I GCGC 3 cut(s) 341, 539, 1392
HinP1I GCGC 3 cut(s) 341, 539, 1392
HinfI GANTC 7 cut(s) 13, 311, 494, 502, 750, 908, 956
HpaII CCGG 6 cut(s) 177, 216, 234, 522, 953, 990
HphI GGTGA 4 cut(s) 205, 536, 1181, 1223
Hpy166II GTNNAC 6 cut(s) 64, 493, 942, 981, 1060, 1303
Hpy188I TCNGA 6 cut(s) 402, 514, 566, 574, 1202, 1411
Hpy188III TCNNGA 6 cut(s) 146, 259, 271, 758, 871, 1086
Hpy8I GTNNAC 6 cut(s) 64, 493, 942, 981, 1060, 1303
Hpy99I CGWCG 1 cut(s) 233
HpyAV CCTTC 7 cut(s) 41, 277, 607, 628, 868, 881, 1286
HpyCH4III ACNGT 6 cut(s) 61, 385, 481, 653, 695, 1100
HpyCH4IV ACGT 1 cut(s) 243
HpyCH4V TGCA 9 cut(s) 64, 105, 734, 809, 942, 1024, 1076, 1287, 1433
HpyF10VI GCNNNNNNNGC 5 cut(s) 347, 545, 614, 939, 1293
HpyF3I CTNAG 4 cut(s) 144, 743, 756, 1297
HpySE526I ACGT 1 cut(s) 243
Hsp92I GRCGYC 1 cut(s) 231
Hsp92II CATG 9 cut(s) 13, 319, 466, 613, 851, 898, 940, 1031, 1198
HspAI GCGC 3 cut(s) 341, 539, 1392
Kzo9I GATC 6 cut(s) 421, 566, 1088, 1241, 1264, 1387
LmnI GCTCC 2 cut(s) 644, 1343
Lsp1109I GCAGC 5 cut(s) 403, 520, 746, 1026, 1234
MaeI CTAG 4 cut(s) 579, 1055, 1349, 1483
MaeII ACGT 1 cut(s) 243
MaeIII GTNAC 5 cut(s) 581, 707, 751, 767, 1030
MalI GATC 6 cut(s) 423, 568, 1090, 1243, 1266, 1389
MboI GATC 6 cut(s) 421, 566, 1088, 1241, 1264, 1387
MboII GAAGA 4 cut(s) 26, 470, 1236, 1379
MfeI CAATTG 1 cut(s) 439
MflI RGATCY 2 cut(s) 421, 1241
MhlI GDGCHC 5 cut(s) 66, 78, 333, 944, 1348
MluCI AATT 8 cut(s) 172, 426, 439, 719, 827, 1066, 1227, 1309
MlyI GAGTC 5 cut(s) 7, 305, 488, 759, 965
MmeI TCCRAC 2 cut(s) 1145, 1180
Mph1103I ATGCAT 1 cut(s) 107
MroXI GAANNNNTTC 1 cut(s) 617
MseI TTAA 2 cut(s) 947, 1428
MslI CAYNNNNRTG 2 cut(s) 336, 1236
MspA1I CMGCKG 1 cut(s) 533
MspI CCGG 6 cut(s) 177, 216, 234, 522, 953, 990
MspR9I CCNGG 8 cut(s) 86, 178, 289, 475, 739, 884, 1008, 1362
MunI CAATTG 1 cut(s) 439
Mva1269I GAATGC 2 cut(s) 343, 560
MvaI CCWGG 7 cut(s) 86, 289, 475, 739, 884, 1008, 1362
MvnI CGCG 2 cut(s) 1087, 1392
MwoI GCNNNNNNNGC 5 cut(s) 347, 545, 614, 939, 1293
NciI CCSGG 1 cut(s) 178
NdeII GATC 6 cut(s) 421, 566, 1088, 1241, 1264, 1387
NlaIII CATG 9 cut(s) 13, 319, 466, 613, 851, 898, 940, 1031, 1198
NlaIV GGNNCC 3 cut(s) 257, 423, 1345
NmuCI GTSAC 1 cut(s) 751
NruI TCGCGA 1 cut(s) 1087
NsiI ATGCAT 1 cut(s) 107
NspI RCATGY 3 cut(s) 851, 898, 1031
OliI CACNNNNGTG 1 cut(s) 1236
PaeI GCATGC 1 cut(s) 851
PceI AGGCCT 1 cut(s) 617
PciI ACATGT 2 cut(s) 894, 1027
PcsI WCGNNNNNNNCGW 2 cut(s) 1084, 1206
PctI GAATGC 2 cut(s) 343, 560
PdmI GAANNNNTTC 1 cut(s) 617
PfeI GAWTC 2 cut(s) 502, 908
PflFI GACNNNGTC 1 cut(s) 242
PflMI CCANNNNNTGG 1 cut(s) 1442
PfoI TCCNGGA 1 cut(s) 882
PkrI GCNGC 7 cut(s) 221, 393, 535, 538, 736, 1016, 1224
PleI GAGTC 5 cut(s) 7, 305, 488, 758, 964
PpsI GAGTC 5 cut(s) 7, 305, 488, 758, 964
PpuMI RGGWCCY 1 cut(s) 1358
PscI ACATGT 2 cut(s) 894, 1027
PshBI ATTAAT 2 cut(s) 947, 1428
Psp5II RGGWCCY 1 cut(s) 1358
Psp6I CCWGG 7 cut(s) 84, 287, 473, 737, 882, 1006, 1360
PspEI GGTNACC 2 cut(s) 707, 767
PspFI CCCAGC 2 cut(s) 444, 834
PspGI CCWGG 7 cut(s) 84, 287, 473, 737, 882, 1006, 1360
PspN4I GGNNCC 3 cut(s) 257, 423, 1345
PspPI GGNCC 3 cut(s) 121, 148, 1358
PspPPI RGGWCCY 1 cut(s) 1358
PstNI CAGNNNCTG 1 cut(s) 512
PsuI RGATCY 2 cut(s) 421, 1241
PsyI GACNNNGTC 1 cut(s) 242
PvuII CAGCTG 1 cut(s) 533
RruI TCGCGA 1 cut(s) 1087
RsaI GTAC 5 cut(s) 411, 899, 914, 980, 1304
RsaNI GTAC 5 cut(s) 410, 898, 913, 979, 1303
RseI CAYNNNNRTG 2 cut(s) 336, 1236
SaqAI TTAA 2 cut(s) 947, 1428
SatI GCNGC 7 cut(s) 220, 392, 534, 537, 735, 1015, 1223
Sau3AI GATC 6 cut(s) 421, 566, 1088, 1241, 1264, 1387
Sau96I GGNCC 3 cut(s) 121, 148, 1358
SchI GAGTC 5 cut(s) 7, 305, 488, 759, 965
ScrFI CCNGG 8 cut(s) 86, 178, 289, 475, 739, 884, 1008, 1362
SduI GDGCHC 5 cut(s) 66, 78, 333, 944, 1348
SgrAI CRCCGGYG 1 cut(s) 215
SinI GGWCC 2 cut(s) 148, 1358
SmiMI CAYNNNNRTG 2 cut(s) 336, 1236
SpeI ACTAGT 1 cut(s) 1054
SphI GCATGC 1 cut(s) 851
Sse9I AATT 8 cut(s) 172, 426, 439, 719, 827, 1066, 1227, 1309
SseBI AGGCCT 1 cut(s) 617
SsiI CCGC 5 cut(s) 69, 166, 219, 536, 1220
SspMI CTAG 4 cut(s) 579, 1055, 1349, 1483
StuI AGGCCT 1 cut(s) 617
StyD4I CCNGG 8 cut(s) 84, 176, 287, 473, 737, 882, 1006, 1360
TaaI ACNGT 6 cut(s) 61, 385, 481, 653, 695, 1100
TaiI ACGT 1 cut(s) 246
TaqI TCGA 6 cut(s) 98, 300, 621, 702, 872, 975
TasI AATT 8 cut(s) 172, 426, 439, 719, 827, 1066, 1227, 1309
TatI WGTACW 3 cut(s) 897, 978, 1302
TauI GCSGC 2 cut(s) 222, 539
TfiI GAWTC 2 cut(s) 502, 908
Tru1I TTAA 2 cut(s) 947, 1428
Tru9I TTAA 2 cut(s) 947, 1428
TscAI CASTG 3 cut(s) 211, 658, 1305
TseFI GTSAC 1 cut(s) 751
TseI GCWGC 5 cut(s) 391, 533, 734, 1014, 1222
Tsp45I GTSAC 1 cut(s) 751
TspDTI ATGAA 4 cut(s) 17, 626, 925, 1032
TspGWI ACGGA 1 cut(s) 380
TspRI CASTG 3 cut(s) 211, 658, 1305
Tth111I GACNNNGTC 1 cut(s) 242
Van91I CCANNNNNTGG 1 cut(s) 1442
VneI GTGCAC 2 cut(s) 62, 940
VpaK11BI GGWCC 2 cut(s) 148, 1358
VspI ATTAAT 2 cut(s) 947, 1428
XagI CCTNNNNNAGG 1 cut(s) 742
XapI RAATTY 2 cut(s) 827, 1227
XceI RCATGY 3 cut(s) 851, 898, 1031
XmnI GAANNNNTTC 1 cut(s) 617
XspI CTAG 4 cut(s) 579, 1055, 1349, 1483
Zsp2I ATGCAT 1 cut(s) 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.