MD10G1075500.v1.1

RNA recognition motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0004608
Physical Location & Seq
Reverse (-)
10717720 .. 10718925
1206 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1075500.v1.1.491

Sequence Viewer

Length: 654 bp
ATGGTTAACCCTAGTCGAGGGTGCCCGAGGGCCTATATTAACGAGGGTTGGGGGATTGCTGGCCCTCGTGCTCTCTTGTTCTCTTGTTCTTTCGACGTAAAATTCGAGTCCCATTTGTCACTAGGGTTACTGGTAGCCATGGCCTCTGCCGAGATCGAGTTCCGTTGCTTCGTCGGAGGGCTCGCATGGGCCACCGACAATGAGGCGCTGGAGAGGGCCTTTTCCCAGTATGGTGAGATCATCGAATCGAAGATCATCAACGACCGTGAGACTGGGAGGTCCAGGGGCTTCGGCTTCGTCACCTTCGGCAGCGAGCAGGCGATGAGGGACGCGATCGAAGGCATGAACGGCCAGAACCTCGACGGCCGCAACATCACCGTCAACGAGGCTCAGTCTCGCGGATCCGGTGGTGGTGGTGGCGGAGGTGGCTACAGCCGCGGAGGCGGCGGTGGCGGCTACGGAGGTGGTGGTGGTGGCTACGGAGGTGGCGGTCGCCGTGAAGGCGGTGGTGGTGGGTACAGCCGTGGAGGTGGTGGCGGTGGATACGGAGGTGGTGGTGGTCGTCGTGAGGGTGGCTATGGAGGCGGTGAAGGTGGCGGTGCTCGCTACTCCAGAGGAGGTGGTGGCGCTGAAGGCGGCAGCTGGAGGAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
RRM_1

Protein Analysis

218

Amino Acids

21.66

Weight (kDa)

7.7

Isoelectric Point (pI)

57.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 57 - 126 6.4e-22 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 277
AccB1I GGYRCC 1 cut(s) 21
AccII CGCG 3 cut(s) 332, 399, 438
AclWI GGATC 2 cut(s) 396, 409
AcoI YGGCCR 2 cut(s) 349, 364
AcsI RAATTY 1 cut(s) 101
AcuI CTGAAG 1 cut(s) 651
AfaI GTAC 1 cut(s) 518
AfiI CCNNNNNNNGG 1 cut(s) 17
AjnI CCWGG 1 cut(s) 281
AluBI AGCT 1 cut(s) 642
AluI AGCT 1 cut(s) 642
Alw21I GWGCWC 2 cut(s) 73, 604
Alw26I GTCTC 2 cut(s) 263, 399
AlwI GGATC 2 cut(s) 396, 409
Ama87I CYCGRG 1 cut(s) 25
AoxI GGCC 7 cut(s) 30, 61, 141, 189, 216, 349, 364
ApeKI GCWGC 2 cut(s) 309, 639
ApoI RAATTY 1 cut(s) 101
ArsI GACNNNNNNTTYG 2 cut(s) 86, 118
AspLEI GCGC 2 cut(s) 208, 629
AspS9I GGNCC 5 cut(s) 30, 62, 189, 216, 279
AsuHPI GGTGA 4 cut(s) 245, 292, 367, 599
AvaI CYCGRG 1 cut(s) 25
AvaII GGWCC 1 cut(s) 279
BaeGI GKGCMC 1 cut(s) 26
BaeI ACNNNNGTAYC 4 cut(s) 508, 535, 541, 568
BamHI GGATCC 1 cut(s) 401
BanI GGYRCC 1 cut(s) 21
BanII GRGCYC 1 cut(s) 183
BauI CACGAG 1 cut(s) 66
Bbv12I GWGCWC 2 cut(s) 73, 604
BbvI GCAGC 1 cut(s) 321
BceAI ACGGC 4 cut(s) 364, 379, 480, 507
BciT130I CCWGG 1 cut(s) 283
BciVI GTATCC 1 cut(s) 536
BcoDI GTCTC 2 cut(s) 263, 399
BfaI CTAG 2 cut(s) 12, 122
BfmI CTRYAG 1 cut(s) 430
BfoI RGCGCY 2 cut(s) 209, 630
BfuI GTATCC 1 cut(s) 536
BglI GCCNNNNNGGC 2 cut(s) 441, 501
BisI GCNGC 7 cut(s) 310, 367, 436, 445, 454, 637, 640
BlsI GCNGC 7 cut(s) 311, 368, 437, 446, 455, 638, 641
Bme1390I CCNGG 1 cut(s) 283
Bme18I GGWCC 1 cut(s) 279
BmeT110I CYCGRG 1 cut(s) 25
BmgT120I GGNCC 5 cut(s) 30, 62, 189, 216, 279
BmiI GGNNCC 2 cut(s) 23, 403
BmrFI CCNGG 1 cut(s) 283
BmrI ACTGGG 2 cut(s) 220, 282
BmuI ACTGGG 2 cut(s) 220, 282
BpmI CTGGAG 2 cut(s) 230, 595
BsaJI CCNNGG 5 cut(s) 26, 138, 282, 436, 523
BsaWI WCCGGW 1 cut(s) 404
Bsc4I CCNNNNNNNGG 1 cut(s) 17
Bse1I ACTGG 3 cut(s) 135, 226, 277
BseBI CCWGG 1 cut(s) 283
BseDI CCNNGG 5 cut(s) 26, 138, 282, 436, 523
BseLI CCNNNNNNNGG 1 cut(s) 17
BseMII CTCAG 1 cut(s) 404
BseNI ACTGG 3 cut(s) 135, 226, 277
BseRI GAGGAG 1 cut(s) 630
BseSI GKGCMC 1 cut(s) 26
BseX3I CGGCCG 1 cut(s) 364
BseXI GCAGC 1 cut(s) 321
Bsh1236I CGCG 3 cut(s) 332, 399, 438
Bsh1285I CGRYCG 4 cut(s) 265, 336, 367, 493
BshFI GGCC 7 cut(s) 32, 63, 143, 191, 218, 351, 366
BshNI GGYRCC 1 cut(s) 21
BsiEI CGRYCG 4 cut(s) 265, 336, 367, 493
BsiHKAI GWGCWC 2 cut(s) 73, 604
BsiHKCI CYCGRG 1 cut(s) 25
BsiSI CCGG 1 cut(s) 405
BslFI GGGAC 2 cut(s) 94, 341
BslI CCNNNNNNNGG 1 cut(s) 17
BsmAI GTCTC 2 cut(s) 263, 399
BsmFI GGGAC 2 cut(s) 94, 341
BsnI GGCC 7 cut(s) 32, 63, 143, 191, 218, 351, 366
BsoBI CYCGRG 1 cut(s) 25
Bsp1286I GDGCHC 4 cut(s) 26, 73, 183, 604
Bsp143I GATC 5 cut(s) 153, 237, 252, 333, 401
Bsp19I CCATGG 1 cut(s) 138
BspANI GGCC 7 cut(s) 32, 63, 143, 191, 218, 351, 366
BspCNI CTCAG 1 cut(s) 403
BspFNI CGCG 3 cut(s) 332, 399, 438
BspLI GGNNCC 2 cut(s) 23, 403
BspPI GGATC 2 cut(s) 396, 409
BspT107I GGYRCC 1 cut(s) 21
BsrI ACTGG 3 cut(s) 135, 226, 277
BssECI CCNNGG 5 cut(s) 26, 138, 282, 436, 523
BssMI GATC 5 cut(s) 153, 237, 252, 333, 401
BssSI CACGAG 1 cut(s) 66
BssT1I CCWWGG 1 cut(s) 138
Bst2BI CACGAG 1 cut(s) 66
Bst2UI CCWGG 1 cut(s) 283
Bst4CI ACNGT 2 cut(s) 266, 379
BstC8I GCNNGC 5 cut(s) 61, 183, 314, 318, 604
BstDEI CTNAG 1 cut(s) 390
BstDSI CCRYGG 3 cut(s) 138, 436, 523
BstENI CCTNNNNNAGG 1 cut(s) 15
BstFNI CGCG 3 cut(s) 332, 399, 438
BstH2I RGCGCY 2 cut(s) 209, 630
BstHHI GCGC 2 cut(s) 208, 629
BstKTI GATC 5 cut(s) 156, 240, 255, 336, 404
BstMAI GTCTC 2 cut(s) 263, 399
BstMBI GATC 5 cut(s) 153, 237, 252, 333, 401
BstMCI CGRYCG 4 cut(s) 265, 336, 367, 493
BstNI CCWGG 1 cut(s) 283
BstSCI CCNGG 1 cut(s) 281
BstSFI CTRYAG 1 cut(s) 430
BstSLI GKGCMC 1 cut(s) 26
BstUI CGCG 3 cut(s) 332, 399, 438
BstV1I GCAGC 1 cut(s) 321
BstX2I RGATCY 1 cut(s) 401
BstYI RGATCY 1 cut(s) 401
BstZI CGGCCG 1 cut(s) 364
BsuI GTATCC 1 cut(s) 536
BsuRI GGCC 7 cut(s) 32, 63, 143, 191, 218, 351, 366
BtgI CCRYGG 3 cut(s) 138, 436, 523
BtgZI GCGATG 1 cut(s) 335
Cac8I GCNNGC 5 cut(s) 61, 183, 314, 318, 604
CfoI GCGC 2 cut(s) 208, 629
Cfr13I GGNCC 5 cut(s) 30, 62, 189, 216, 279
Cfr42I CCGCGG 1 cut(s) 439
CseI GACGC 1 cut(s) 338
Csp6I GTAC 1 cut(s) 517
CviAII CATG 3 cut(s) 139, 186, 343
CviQI GTAC 1 cut(s) 517
DdeI CTNAG 1 cut(s) 390
DpnI GATC 5 cut(s) 155, 239, 254, 335, 403
DpnII GATC 5 cut(s) 153, 237, 252, 333, 401
DrdI GACNNNNNNGTC 1 cut(s) 277
DseDI GACNNNNNNGTC 1 cut(s) 277
EaeI YGGCCR 2 cut(s) 349, 364
EagI CGGCCG 1 cut(s) 364
EciI GGCGGA 1 cut(s) 435
EclXI CGGCCG 1 cut(s) 364
Eco130I CCWWGG 1 cut(s) 138
Eco24I GRGCYC 1 cut(s) 183
Eco47I GGWCC 1 cut(s) 279
Eco52I CGGCCG 1 cut(s) 364
Eco57I CTGAAG 1 cut(s) 651
Eco88I CYCGRG 1 cut(s) 25
EcoNI CCTNNNNNAGG 1 cut(s) 15
EcoO109I RGGNCCY 2 cut(s) 30, 216
EcoRII CCWGG 1 cut(s) 281
EcoT14I CCWWGG 1 cut(s) 138
EcoT38I GRGCYC 1 cut(s) 183
ErhI CCWWGG 1 cut(s) 138
FaeI CATG 3 cut(s) 142, 189, 346
FaiI YATR 6 cut(s) 36, 140, 187, 231, 344, 579
FaqI GGGAC 2 cut(s) 94, 341
FatI CATG 3 cut(s) 138, 185, 342
Fnu4HI GCNGC 7 cut(s) 310, 367, 436, 445, 454, 637, 640
FriOI GRGCYC 1 cut(s) 183
Fsp4HI GCNGC 7 cut(s) 310, 367, 436, 445, 454, 637, 640
FspBI CTAG 2 cut(s) 12, 122
GlaI GCGC 2 cut(s) 207, 628
GluI GCNGC 7 cut(s) 310, 367, 436, 445, 454, 637, 640
GsuI CTGGAG 2 cut(s) 230, 595
HaeII RGCGCY 2 cut(s) 209, 630
HaeIII GGCC 7 cut(s) 32, 63, 143, 191, 218, 351, 366
HapII CCGG 1 cut(s) 405
HgaI GACGC 1 cut(s) 338
HhaI GCGC 2 cut(s) 208, 629
Hin1II CATG 3 cut(s) 142, 189, 346
Hin6I GCGC 2 cut(s) 206, 627
HinP1I GCGC 2 cut(s) 206, 627
HincII GTYRAC 2 cut(s) 7, 382
HindII GTYRAC 2 cut(s) 7, 382
HinfI GANTC 2 cut(s) 107, 245
HpaI GTTAAC 1 cut(s) 7
HpaII CCGG 1 cut(s) 405
HphI GGTGA 4 cut(s) 245, 292, 367, 599
Hpy166II GTNNAC 2 cut(s) 7, 382
Hpy188I TCNGA 1 cut(s) 176
Hpy188III TCNNGA 2 cut(s) 566, 612
Hpy8I GTNNAC 2 cut(s) 7, 382
Hpy99I CGWCG 4 cut(s) 98, 176, 365, 567
HpyAV CCTTC 5 cut(s) 313, 332, 494, 584, 626
HpyCH4III ACNGT 2 cut(s) 266, 379
HpyCH4IV ACGT 1 cut(s) 96
HpyF3I CTNAG 1 cut(s) 390
HpySE526I ACGT 1 cut(s) 96
Hsp92II CATG 3 cut(s) 142, 189, 346
HspAI GCGC 2 cut(s) 206, 627
KspAI GTTAAC 1 cut(s) 7
KspI CCGCGG 1 cut(s) 439
Kzo9I GATC 5 cut(s) 153, 237, 252, 333, 401
Lsp1109I GCAGC 1 cut(s) 321
MaeI CTAG 2 cut(s) 12, 122
MaeII ACGT 1 cut(s) 96
MaeIII GTNAC 3 cut(s) 117, 126, 298
MalI GATC 5 cut(s) 155, 239, 254, 335, 403
MboI GATC 5 cut(s) 153, 237, 252, 333, 401
MboII GAAGA 1 cut(s) 262
MflI RGATCY 1 cut(s) 401
MhlI GDGCHC 4 cut(s) 26, 73, 183, 604
MluCI AATT 1 cut(s) 101
MlyI GAGTC 1 cut(s) 116
MmeI TCCRAC 1 cut(s) 154
MseI TTAA 2 cut(s) 6, 39
MspA1I CMGCKG 2 cut(s) 438, 642
MspI CCGG 1 cut(s) 405
MspR9I CCNGG 1 cut(s) 283
MvaI CCWGG 1 cut(s) 283
MvnI CGCG 3 cut(s) 332, 399, 438
NcoI CCATGG 1 cut(s) 138
NdeII GATC 5 cut(s) 153, 237, 252, 333, 401
NlaIII CATG 3 cut(s) 142, 189, 346
NlaIV GGNNCC 2 cut(s) 23, 403
NmeAIII GCCGAG 1 cut(s) 175
NmuCI GTSAC 2 cut(s) 117, 298
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PfeI GAWTC 1 cut(s) 245
PkrI GCNGC 7 cut(s) 311, 368, 437, 446, 455, 638, 641
Ple19I CGATCG 1 cut(s) 336
PleI GAGTC 1 cut(s) 115
PpsI GAGTC 1 cut(s) 115
Psp6I CCWGG 1 cut(s) 281
PspGI CCWGG 1 cut(s) 281
PspN4I GGNNCC 2 cut(s) 23, 403
PspPI GGNCC 5 cut(s) 30, 62, 189, 216, 279
PsuI RGATCY 1 cut(s) 401
PvuI CGATCG 1 cut(s) 336
PvuII CAGCTG 1 cut(s) 642
RsaI GTAC 1 cut(s) 518
RsaNI GTAC 1 cut(s) 517
SacII CCGCGG 1 cut(s) 439
SaqAI TTAA 2 cut(s) 6, 39
SatI GCNGC 7 cut(s) 310, 367, 436, 445, 454, 637, 640
Sau3AI GATC 5 cut(s) 153, 237, 252, 333, 401
Sau96I GGNCC 5 cut(s) 30, 62, 189, 216, 279
SchI GAGTC 1 cut(s) 116
ScrFI CCNGG 1 cut(s) 283
SduI GDGCHC 4 cut(s) 26, 73, 183, 604
SfcI CTRYAG 1 cut(s) 430
Sfr303I CCGCGG 1 cut(s) 439
SgrBI CCGCGG 1 cut(s) 439
SinI GGWCC 1 cut(s) 279
Sse9I AATT 1 cut(s) 101
SspMI CTAG 2 cut(s) 12, 122
StyD4I CCNGG 1 cut(s) 281
StyI CCWWGG 1 cut(s) 138
TaaI ACNGT 2 cut(s) 266, 379
TaiI ACGT 1 cut(s) 99
TaqI TCGA 8 cut(s) 16, 93, 105, 156, 243, 248, 336, 360
TasI AATT 1 cut(s) 101
TauI GCSGC 5 cut(s) 369, 438, 447, 456, 639
TfiI GAWTC 1 cut(s) 245
Tru1I TTAA 2 cut(s) 6, 39
Tru9I TTAA 2 cut(s) 6, 39
TseFI GTSAC 2 cut(s) 117, 298
TseI GCWGC 2 cut(s) 309, 639
Tsp45I GTSAC 2 cut(s) 117, 298
TspDTI ATGAA 1 cut(s) 359
TspGWI ACGGA 4 cut(s) 152, 474, 495, 561
VpaK11BI GGWCC 1 cut(s) 279
XagI CCTNNNNNAGG 1 cut(s) 15
XapI RAATTY 1 cut(s) 101
XspI CTAG 2 cut(s) 12, 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.