Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0001783

Physical Location & Seq

Genomic Coordinates Reverse (-)

34755303 .. 34769742

Gene Structure 14440 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1255500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4728 bp

ATGGGCCTTTGCTGCTACCGGATATGGATGATCAAGATGAGTTCAAAAGCCCGGAGGTTTCGATTGAGGACGAATTACTACAATTACATTTTGGGTTTGTTGGCTGGTTACTTCACTGCACAGCCCTTACTAAGGTTGCTGATGGGCATGTCATATTTTAACTTCAACACACAATCCGGCTCTGCTCCTTTTGAGATGACTTCTGCAGTCATTGAGGCAATTGCTTGGTGCTCCATGCTAATTATGATTGGCTTGGAGACTAAGATATACGTAAAGGAATTCCGGTGGTATGTAAGGTTTGGAGTCATTTATGTCTTGGTGGGAGATGCTGTGGTGCTAAGCCTTATGCTTTCAGTGGCAGATTACTACAACAGAGGTACTCTGTATCTGTATATAAGCACGGTCTGTTGCCAGGTGCTGTTTGGAATTCTACTTCTCGTTTATATTCCTAATTTGGATCCTTATCCGGGTTACATTCCTCTTCAATCTGAGCCTCTTGATAATGTCGAATATGAAGCACTACCTGGAGAAGAACAAATTTTCCCTGAAAGACATGTTAATATATTTTCCAGAATATATTTCGGATGGATGACTCCACTCATGCAGCTAGGGTATCGAAAACCCATCACCGAATCAGATGTTTGGAAGTTGGATGCGTGGGATCAGACTGAGACACTAATTAATAAGTTCCAGACATGTTGGGATAAGGAATCTCAAAGGCCCAAGCCATGGCTTCTAAGAGCATTGAACTGTAGCCTTGGAGGAAGGTTCTGGTGGGGAGGCTTATTCAAGATTGGAAATGATCTTTCTCAGTTTGCGGGTCCGGTTCTACTAAGTCATCTCTTGCAGTCAATGCAACAAGGCGATCCGACTTGGATTGGTTACATCTATGCTTTCTTAATTTTCACTGGTGTGTCACTTGGTGTGCTCTGTGAAGCTCAGTATTTCCAGAATGTTCAGCGGGTCGGTTTTAGGCTCAGATCAACTTTGGTTGCTGCTATATTCCGTAAATCTATAAGAATAACTCATGAAGGCCGTAAGAAGTTTCCATCTGGGAAGATAACAAATATGATGTCAACAGATGCTAATGCAGTCCAGCAAATATGTCAACAACTTCATGGATTGTGGTCAGCTCCTTTTCGTATCACTGTAGCTATGGTTCTTCTTTACCAGCAACTAGGTGTGGCATCACTCATTGGATCTGGAATGCTGGTTCTCATGATCCCCTTACAGACATTTGTGATCAGCAAAATGCGAAAACTGATAAAAGATGGACTACAGCAAACTGACAAGAGAGTTGGCCTCATGAATGAAATTTTGGCAGCCATGGATACAGTGAAATGTTACGCCTGGGAGACAAGTTTCCAACACCGAGTTCAAAGTATAAGGAATGATGAGTTGTCAAGGTTCCGTAAAGCGCAATTACTATCTTCTTTTAACAGTTTTATACTGAACAGCATCCCAGTTTTTGTGACATTAACATCGTTCGGGGTGTTCACTGCTCTTGGTGGCGAATTGACACCTGCAAGGGCATTTACGTCCCTTTCTCTTTTTGCAGTGCTACGGTTTCCTCTAATCATGCTGCCTAGCTTACTAAGTCAGATTGTGAATGCAAATGTATCGTTACAACGTTTGGAGGAACTATTTTTAACTGAGGAGAGAATTCTAGTACCGAACTTGCCTCTTGAACCAGGGCTTCCAGCCATCTCAATTAAGGATGGGTACTTTTCATGGGATTCAGAGGCAGAGAATCCCACATTATCGAACATCAACTTAGATATACCAGTTGGCAGCTTAGTAGCAGTTGTTGGTGGAACTGGAGAAGGGAAGACATCGCTGGTATCAGCCATGCTTGGTGAGCTGCCTCCCAGAACAGATGCCAGTGTTGTAGTCAGGGGGACTGTAGCTTATGTTCCCCAAGTTTCATGGATTTTCAACGCTACTGTACGCGAAAATATATTATTTGGATCTAAATTCGAATCTGAACGATATTGGAAGGCCATTGATTTGACCGAGTTACAGCACGATCTAGACTTACTTCCAGGTCGAGATCTAACAGAGATAGGTGAAAGAGGGGTGAATATTAGCGGCGGTCAAAAGCAAAGAGTTTCAATGGCTAGAGCTGTATATTCTGATTCAGATGTTTACATATTTGACGACCCGTTAAGTGCTCTAGATGCTCATGTGGCGAGAGAGGTTTTCAACCACTGTATCAAGGAAGAGTTGCAAGGGAAAACTCGGGTGCTGGTCACAAATCAGCTACATTTTCTTCCTCAAGTGGATCGTATTATTTTAGTCTCTGAAGGTATGATTAAAGGTGAGGGAACCTTTAAGGAGCTCTCTGAAAGCAGTACGCTGTTCCAGAAGCTGATGGAAAATGCAGGGAAATTGGAAGCACATGTCGAAGAAAAGGAAGAGAGTGAAAATGATAACCATGAGAGCTCAACACCTACTTCCAATGGGGTGTCGAATGAGTTGCCAAAAGATGCAAGCAACCCAGAGAAAGGGAAAGGGGCGAAATCCGTACTCATTAAACAAGAGGAAAGGGAAACAGGCATTGTCAGTTGGGAAATTCTAATGAGGTACAAAAATGCATTAGGAGGACTATGGGTGGTCATGGTTCTATTTGCGTGTTATACATTAACAGAAGTTCTTCGAGTTTCGAGCAGCACATGGTTAAGTGTTTGGACATCAAAAAGCACATCGAAGAGTTACAAACCTGGATTCTTCATTCTTGTGTATGGTCTTCTGTCATTTGGTCAGGTAACAGTGACACTGACAAACTCTGTTTGGTTAATCACATCAAGCCTTCGTGCAGCCAGAAGATTGCACGATGCTCTGCTTAATGCTATAATGAGAGCTCCAATGGTATTCTTCCACACTAATCCAACAGGACGGATAATCAATAGGTTTGCGAAAGATCTGGGTGACATAGATCGCGGTGTAGCAATGGTTGTGAACATGTTTATGGGTCAAGTGTGGCAGCTCTTTGCAACTTTTGTGCTCATAGGCATTGTGAGCACAATATCCCTCTGGGCCATAATGCCACTTCTGATTTTGTTCTATGCAGCCTATCTTTTTTATCAGAGCACATCCCGTGAAGTGAAACGCTTGGATTCCATTAGCAGATCTCCAGTTTATGCACAATTTGGAGAAGCATTGAATGGTTTGTCAACCATTCGCGCCTACAAAGCGTATGATCGGATGGCCAACATTAGCGGAAGGTCCATGGATAAGAGCATCAGATTTACCCTTGTGAATATGAGTTCAAATCGCTGGCTTACGATTAGGTTGGAAACGCTCGGAGGTCTGATGATTTGGTTGATAGCAAGCTTTGCTGTCATGCAAAACGGAAGAGCAGAAGACAAAGTAGCATTTGCATCTACAATGGGTCTACTTCTCACTTATACTTTAAATATCACAAATTTGTTGAGCGGTGTTCTTAGGACAGCCAGCCTGGCTGAAAATAGTTTAAATGCCGTTGAGCGTGTTGGATCCTATATAGAACTGCCTTCTGAGGCGCCGGCTGTAATTGAAAGCAACCGTCCCCAACCTGGGTGGCCATCATCAGGATCAATCGAGTTTGAAGATGTCGTCCTGCGTTATAGGCCTGGACTTCCTCCGGTATTGCACGGATTATCTTTTACAGTTTCTGCGAGTGAAAAGCTAGGAATAGTCGGAAGAACTGGTGCAGGGAAATCTAGCATGATTAATGCATTGTTTAGAATAGTAGAAATCGAAAAGGGAAGAATCTTGATAGATAGTTGTGATATTTCCAAGTTTGGACTGACGGATTTGCGAAAAGTTCTTAGTATCATACCGCAGTCACCCGTTCTTTTTTCAGGAACCGTGCGGTTTAACCTTGATCCCTTCAGCGAGCATAGCGACGCTGACCTTTGGGAAGCTCTAGAGAGGGCACATCTAAAGGATGTCATTAGGCGGAATTCTCTCGGTCTGGATGCTGAGGTTTCCGAAGGAGGTGAAAACTTTAGCGTTGGGCAGAGGCAACTATTAAGTCTTGCTAGAGCATTGCTGCGAAGATCAAAGATTCTTGTTCTTGATGAAGCAACTGCTGCTGTCGACGTTGGGACTGATGCTCTTATCCAGAAAACAATTCGCGAAGAGTTCAAATCGTGCACCATGCTCGTTATTGCTCACAGATTAAATACCATAATTGACTGTGATCAAATTCTGGTTCTGGATGCCGGTCAGGTCCTGGAACATGACTCCCCAGACAAACTCCTATCAAACGAAGAGAGTGCCTTCTCCAAGATGGTCAAGAGCACGGGACCTGCAAATGCTGAGTACTTGCGCGGCCTGGTATTTGGAGGTAAACAGAATAAGGTGGTTAGAGACCAAACGAAGCAGCTGGCTGGTCAGAGCCAGAGGGGATGGTTAGCCTCTTCGCGCTGGGCCGCAGCTGCCCAGTTAGCCTTAGCTGTTAGTCTCACTTCTTCGCAAAATGACCTTCGAGGAATGGACATTGAAGACAAAAACAATATCCTCATGAAAACAAATGACGCAGTAATAACACTTCAGGGAGTTTTGGAAGGGAAGCATGACAAAGATATAGACGACACACTGAATCAACACCATATACCTAGGGAAGGATGGTGGTCGGCTTTCTTCAGAGTAGTAGAAGGTATGGCTGTGATGGGCAGGCTGGCTCACAACAGACTTCAGCCGTTAGAAGACGACTTTGAAAACAAAAAAATAGATTGGAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006810 GO:0008150 GO:0015399 GO:0015405 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022804 GO:0022857 GO:0031090 GO:0042623 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko01523 ko01524 ko02010 ko04976 map01523 map01524 map02010 map04976

Pfam Domains

Protein Families

ABC_membrane ABC_tran

Protein Analysis

1576

Amino Acids

176.3

Weight (kDa)

7.55

Isoelectric Point (pI)

40.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ABC_membrane	PF00664	260 - 525	1.1e-38	ABC transporter transmembrane region
ABC_tran	PF00005	587 - 722	8.6e-18	ABC transporter
ABC_membrane	PF00664	873 - 1130	9.9e-32	ABC transporter transmembrane region
ABC_tran	PF00005	1210 - 1358	2e-26	ABC transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0001783)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30410 AT1G30410 AT1G30410 AT1G30410 AT1G30410 AT1G30410 AT1G30420 AT1G30420
fragaria_vesca	FvH4_2g14981 FvH4_2g14981 FvH4_2g14981 FvH4_2g14981 FvH4_2g14981 FvH4_2g14981 FvH4_2g14981 FvH4_3g09710 FvH4_3g09720
malus_domestica	MD10G1255300.v1.1 MD10G1255500.v1.1
prunus_persica	Prupe.4G088600_v2.0.a1 Prupe.4G088600_v2.0.a1
pyrus_communis	pycom10g21050
rosa_chinensis	RchiOBHm_Chr3g0492011 RchiOBHm_Chr5g0015441 RchiOBHm_Chr5g0015451 RchiOBHm_Chr5g0015481 RchiOBHm_Chr5g0015491
rosa_laevigata	RLG00000032199 RLG00000032201 RLG00000032202
rosa_multiflora	Rmu_sc0019960.1_g000002
rosa_roxburghii	Rroxscaffold_1G00061020 Rroxscaffold_1G00061040 Rroxscaffold_1G00061050 Rroxscaffold_6G00392640
rosa_rugosa	Rorug03G0251100 Rorug05G0027400 Rorug05G0027500 Rorug05G0027700
rosa_samantha	Rh3AG301500 Rh5AG121000 Rh5AG121100 Rh5CG130600 Rh5DG118900 Rh5DG119000
rosa_wichuraiana	Rw5G010450 Rw5G010470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1531
Acc36I	ACCTGC	2 cut(s)	1531, 4300
AccB1I	GGYRCC	1 cut(s)	3520
AccB7I	CCANNNNNTGG	1 cut(s)	729
AccBSI	CCGCTC	1 cut(s)	3435
AccI	GTMKAC	2 cut(s)	3394, 4080
AccII	CGCG	6 cut(s)	1950, 2940, 3183, 4119, 4314, 4408
AclI	AACGTT	1 cut(s)	1630
AcoI	YGGCCR	2 cut(s)	3207, 3560
AcuI	CTGAAG	5 cut(s)	2322, 3856, 4520, 4612, 4664
AcyI	GRCGYC	1 cut(s)	3521
AdeI	CACNNNGTG	1 cut(s)	923
AfaI	GTAC	8 cut(s)	379, 1671, 1724, 1947, 2353, 2526, 2585, 4307
AfiI	CCNNNNNNNGG	8 cut(s)	132, 467, 729, 2504, 3554, 3555, 3569, 4607
AflIII	ACRYGT	4 cut(s)	553, 695, 2398, 2961
AjuI	GAANNNNNNNTTGG	4 cut(s)	1301, 1333, 1359, 1391
AleI	CACNNNNGTG	1 cut(s)	911
AloI	GAACNNNNNNTCC	4 cut(s)	525, 557, 1197, 1229
Alw26I	GTCTC	6 cut(s)	251, 665, 1349, 2302, 4347, 4451
Alw44I	GTGCAC	1 cut(s)	4135
AlwNI	CAGNNNCTG	4 cut(s)	418, 2368, 3653, 4216
Ama87I	CYCGRG	1 cut(s)	2238
ApaLI	GTGCAC	1 cut(s)	4135
ArsI	GACNNNNNNTTYG	2 cut(s)	1058, 1090
AseI	ATTAAT	2 cut(s)	681, 3711
Asp700I	GAANNNNTTC	1 cut(s)	2652
AspA2I	CCTAGG	1 cut(s)	4601
AspLEI	GCGC	5 cut(s)	1420, 3185, 3523, 4314, 4410
AspS9I	GGNCC	8 cut(s)	4, 720, 821, 3036, 3225, 4213, 4289, 4413
AsuC2I	CCSGG	2 cut(s)	52, 468
AsuHPI	GGTGA	8 cut(s)	619, 1868, 2078, 2089, 2330, 2939, 3819, 3992
AsuII	TTCGAA	1 cut(s)	1977
AvaI	CYCGRG	1 cut(s)	2238
AvaII	GGWCC	4 cut(s)	821, 3225, 4213, 4289
AvrII	CCTAGG	1 cut(s)	4601
BaeGI	GKGCMC	2 cut(s)	3919, 4139
BaeI	ACNNNNGTAYC	2 cut(s)	2194, 2227
BalI	TGGCCA	2 cut(s)	3209, 3562
BamHI	GGATCC	2 cut(s)	457, 3494
BanI	GGYRCC	1 cut(s)	3520
BanII	GRGCYC	3 cut(s)	2340, 2444, 2863
BarI	GAAGNNNNNNTAC	2 cut(s)	4518, 4550
BbsI	GAAGAC	5 cut(s)	1835, 2738, 3369, 4494, 4698
BbvCI	CCTCAGC	1 cut(s)	3963
BceAI	ACGGC	3 cut(s)	1020, 3464, 4669
BcgI	CGANNNNNNTGC	8 cut(s)	1602, 1636, 2458, 2492, 4126, 4160, 4241, 4275
BciVI	GTATCC	1 cut(s)	1324
BclI	TGATCA	3 cut(s)	30, 1242, 4183
BcnI	CCSGG	2 cut(s)	52, 468
BcoDI	GTCTC	6 cut(s)	251, 665, 1349, 2302, 4347, 4451
BfmI	CTRYAG	5 cut(s)	204, 751, 1149, 1277, 1902
BfoI	RGCGCY	1 cut(s)	3524
BfuAI	ACCTGC	2 cut(s)	1531, 4300
BfuI	GTATCC	1 cut(s)	1324
BglI	GCCNNNNNGGC	1 cut(s)	3458
BglII	AGATCT	3 cut(s)	2050, 2920, 3128
BlnI	CCTAGG	1 cut(s)	4601
BlpI	GCTNAGC	1 cut(s)	338
BmcAI	AGTACT	1 cut(s)	4307
Bme18I	GGWCC	4 cut(s)	821, 3225, 4213, 4289
BmeT110I	CYCGRG	1 cut(s)	2238
BmgT120I	GGNCC	8 cut(s)	4, 720, 821, 3036, 3225, 4213, 4289, 4413
BmiI	GGNNCC	8 cut(s)	459, 822, 1409, 2326, 3496, 3522, 3847, 4290
BmrI	ACTGGG	2 cut(s)	1457, 4420
BmuI	ACTGGG	2 cut(s)	1457, 4420
BpiI	GAAGAC	5 cut(s)	1835, 2738, 3369, 4494, 4698
BplI	GAGNNNNNCTC	2 cut(s)	1287, 1319
BpmI	CTGGAG	3 cut(s)	546, 1839, 3117
Bpu10I	CCTNAGC	2 cut(s)	3963, 4435
Bpu1102I	GCTNAGC	1 cut(s)	338
Bpu14I	TTCGAA	1 cut(s)	1977
BpuEI	CTTGAG	1 cut(s)	2259
BpuMI	CCSGG	2 cut(s)	52, 468
BsaAI	YACGTR	1 cut(s)	271
BsaBI	GATNNNNATC	1 cut(s)	462
BsaHI	GRCGYC	1 cut(s)	3521
BsaI	GGTCTC	1 cut(s)	4347
BsaJI	CCNNGG	8 cut(s)	728, 757, 1326, 1350, 1691, 3228, 3554, 4601
BsaWI	WCCGGW	4 cut(s)	18, 282, 823, 3622
BsaXI	ACNNNNNCTCC	2 cut(s)	315, 345
Bsc4I	CCNNNNNNNGG	8 cut(s)	132, 467, 729, 2504, 3554, 3555, 3569, 4607
Bse118I	RCCGGY	2 cut(s)	3523, 4205
Bse1I	ACTGG	8 cut(s)	913, 1463, 1784, 1822, 1881, 3134, 3691, 4426
Bse3DI	GCAATG	2 cut(s)	2955, 4028
Bse8I	GATNNNNATC	1 cut(s)	462
BseDI	CCNNGG	8 cut(s)	728, 757, 1326, 1350, 1691, 3228, 3554, 4601
BseJI	GATNNNNATC	1 cut(s)	462
BseLI	CCNNNNNNNGG	8 cut(s)	132, 467, 729, 2504, 3554, 3555, 3569, 4607
BseMI	GCAATG	2 cut(s)	2955, 4028
BseMII	CTCAG	9 cut(s)	480, 660, 824, 953, 991, 1644, 3507, 3954, 4293
BseNI	ACTGG	8 cut(s)	913, 1463, 1784, 1822, 1881, 3134, 3691, 4426
BseRI	GAGGAG	1 cut(s)	1670
BseSI	GKGCMC	2 cut(s)	3919, 4139
BseYI	CCCAGC	1 cut(s)	4410
BsgI	GTGCAG	3 cut(s)	102, 2835, 3711
Bsh1236I	CGCG	6 cut(s)	1950, 2940, 3183, 4119, 4314, 4408
BshNI	GGYRCC	1 cut(s)	3520
BsiHKCI	CYCGRG	1 cut(s)	2238
BsiSI	CCGG	9 cut(s)	19, 52, 177, 283, 467, 824, 3524, 3623, 4206
BslFI	GGGAC	5 cut(s)	1525, 1912, 3531, 4102, 4302
BslI	CCNNNNNNNGG	8 cut(s)	132, 467, 729, 2504, 3554, 3555, 3569, 4607
BsmAI	GTCTC	6 cut(s)	251, 665, 1349, 2302, 4347, 4451
BsmFI	GGGAC	5 cut(s)	1525, 1912, 3531, 4102, 4302
BsmI	GAATGC	2 cut(s)	1212, 1615
Bso31I	GGTCTC	1 cut(s)	4347
BsoBI	CYCGRG	1 cut(s)	2238
Bsp119I	TTCGAA	1 cut(s)	1977
Bsp1720I	GCTNAGC	1 cut(s)	338
Bsp19I	CCATGG	3 cut(s)	728, 1326, 3228
Bsp68I	TCGCGA	1 cut(s)	4119
BspCNI	CTCAG	9 cut(s)	481, 661, 823, 952, 990, 1645, 3508, 3955, 4294
BspFNI	CGCG	6 cut(s)	1950, 2940, 3183, 4119, 4314, 4408
BspHI	TCATGA	4 cut(s)	1027, 1218, 1305, 4506
BspLI	GGNNCC	8 cut(s)	459, 822, 1409, 2326, 3496, 3522, 3847, 4290
BspMAI	CTGCAG	1 cut(s)	208
BspMI	ACCTGC	2 cut(s)	1531, 4300
BspQI	GCTCTTC	1 cut(s)	3349
BspT104I	TTCGAA	1 cut(s)	1977
BspT107I	GGYRCC	1 cut(s)	3520
BspTNI	GGTCTC	1 cut(s)	4347
BsrBI	CCGCTC	1 cut(s)	3435
BsrDI	GCAATG	2 cut(s)	2955, 4028
BsrFI	RCCGGY	2 cut(s)	3523, 4205
BsrI	ACTGG	8 cut(s)	913, 1463, 1784, 1822, 1881, 3134, 3691, 4426
BssAI	RCCGGY	2 cut(s)	3523, 4205
BssECI	CCNNGG	8 cut(s)	728, 757, 1326, 1350, 1691, 3228, 3554, 4601
BssNI	GRCGYC	1 cut(s)	3521
BssT1I	CCWWGG	5 cut(s)	728, 757, 1326, 3228, 4601
Bst6I	CTCTTC	8 cut(s)	486, 2214, 2409, 2702, 3349, 4116, 4248, 4408
BstACI	GRCGYC	1 cut(s)	3521
BstAPI	GCANNNNNTGC	3 cut(s)	853, 3335, 4073
BstBAI	YACGTR	1 cut(s)	271
BstBI	TTCGAA	1 cut(s)	1977
BstC8I	GCNNGC	9 cut(s)	2491, 3278, 3331, 3454, 3525, 3878, 4371, 4661, 4665
BstDSI	CCRYGG	3 cut(s)	728, 1326, 3228
BstENI	CCTNNNNNAGG	2 cut(s)	130, 4605
BstFNI	CGCG	6 cut(s)	1950, 2940, 3183, 4119, 4314, 4408
BstH2I	RGCGCY	1 cut(s)	3524
BstHHI	GCGC	5 cut(s)	1420, 3185, 3523, 4314, 4410
BstMAI	GTCTC	6 cut(s)	251, 665, 1349, 2302, 4347, 4451
BstNSI	RCATGY	5 cut(s)	151, 557, 699, 2402, 2965
BstSFI	CTRYAG	5 cut(s)	204, 751, 1149, 1277, 1902
BstSLI	GKGCMC	2 cut(s)	3919, 4139
BstSNI	TACGTA	1 cut(s)	271
BstUI	CGCG	6 cut(s)	1950, 2940, 3183, 4119, 4314, 4408
BstV2I	GAAGAC	5 cut(s)	1835, 2738, 3369, 4494, 4698
BstX2I	RGATCY	7 cut(s)	457, 1199, 1967, 2050, 2920, 3128, 3494
BstYI	RGATCY	7 cut(s)	457, 1199, 1967, 2050, 2920, 3128, 3494
BsuI	GTATCC	1 cut(s)	1324
BtgI	CCRYGG	3 cut(s)	728, 1326, 3228
BtgZI	GCGATG	1 cut(s)	1818
BtsI	GCAGTG	3 cut(s)	114, 1497, 1563
BtuMI	TCGCGA	1 cut(s)	4119
BveI	ACCTGC	2 cut(s)	1531, 4300
Cac8I	GCNNGC	9 cut(s)	2491, 3278, 3331, 3454, 3525, 3878, 4371, 4661, 4665
CaiI	CAGNNNCTG	4 cut(s)	418, 2368, 3653, 4216
CciI	TCATGA	4 cut(s)	1027, 1218, 1305, 4506
CfoI	GCGC	5 cut(s)	1420, 3185, 3523, 4314, 4410
Cfr10I	RCCGGY	2 cut(s)	3523, 4205
Cfr13I	GGNCC	8 cut(s)	4, 720, 821, 3036, 3225, 4213, 4289, 4413
CseI	GACGC	2 cut(s)	3896, 4529
Csp6I	GTAC	8 cut(s)	378, 1670, 1723, 1946, 2352, 2525, 2584, 4306
CspCI	CAANNNNNGTGG	2 cut(s)	3539, 3574
CviQI	GTAC	8 cut(s)	378, 1670, 1723, 1946, 2352, 2525, 2584, 4306
DinI	GGCGCC	1 cut(s)	3522
DraI	TTTAAA	2 cut(s)	3414, 3474
DraIII	CACNNNGTG	1 cut(s)	923
EaeI	YGGCCR	2 cut(s)	3207, 3560
Eam1104I	CTCTTC	8 cut(s)	486, 2214, 2409, 2702, 3349, 4116, 4248, 4408
EarI	CTCTTC	8 cut(s)	486, 2214, 2409, 2702, 3349, 4116, 4248, 4408
EciI	GGCGGA	1 cut(s)	3955
Ecl136II	GAGCTC	3 cut(s)	2338, 2442, 2861
Eco105I	TACGTA	1 cut(s)	271
Eco130I	CCWWGG	5 cut(s)	728, 757, 1326, 3228, 4601
Eco147I	AGGCCT	1 cut(s)	3610
Eco24I	GRGCYC	3 cut(s)	2340, 2444, 2863
Eco31I	GGTCTC	1 cut(s)	4347
Eco47I	GGWCC	4 cut(s)	821, 3225, 4213, 4289
Eco53kI	GAGCTC	3 cut(s)	2338, 2442, 2861
Eco57I	CTGAAG	5 cut(s)	2322, 3856, 4520, 4612, 4664
Eco88I	CYCGRG	1 cut(s)	2238
EcoICRI	GAGCTC	3 cut(s)	2338, 2442, 2861
EcoNI	CCTNNNNNAGG	2 cut(s)	130, 4605
EcoO109I	RGGNCCY	2 cut(s)	4213, 4289
EcoRI	GAATTC	4 cut(s)	278, 426, 1662, 3943
EcoT14I	CCWWGG	5 cut(s)	728, 757, 1326, 3228, 4601
EcoT22I	ATGCAT	2 cut(s)	2596, 3718
EcoT38I	GRGCYC	3 cut(s)	2340, 2444, 2863
EgeI	GGCGCC	1 cut(s)	3522
EheI	GGCGCC	1 cut(s)	3522
ErhI	CCWWGG	5 cut(s)	728, 757, 1326, 3228, 4601
FaqI	GGGAC	5 cut(s)	1525, 1912, 3531, 4102, 4302
FauI	CCCGC	2 cut(s)	811, 954
FbaI	TGATCA	3 cut(s)	30, 1242, 4183
FblI	GTMKAC	2 cut(s)	3394, 4080
FriOI	GRGCYC	3 cut(s)	2340, 2444, 2863
GlaI	GCGC	5 cut(s)	1419, 3184, 3522, 4313, 4409
GsaI	CCCAGC	1 cut(s)	4414
GsuI	CTGGAG	3 cut(s)	546, 1839, 3117
HaeII	RGCGCY	1 cut(s)	3524
HapII	CCGG	9 cut(s)	19, 52, 177, 283, 467, 824, 3524, 3623, 4206
HgaI	GACGC	2 cut(s)	3896, 4529
HhaI	GCGC	5 cut(s)	1420, 3185, 3523, 4314, 4410
Hin1I	GRCGYC	1 cut(s)	3521
Hin6I	GCGC	5 cut(s)	1418, 3183, 3521, 4312, 4408
HinP1I	GCGC	5 cut(s)	1418, 3183, 3521, 4312, 4408
HincII	GTYRAC	4 cut(s)	1077, 1109, 3174, 4081
HindII	GTYRAC	4 cut(s)	1077, 1109, 3174, 4081
HindIII	AAGCTT	1 cut(s)	3331
HpaII	CCGG	9 cut(s)	19, 52, 177, 283, 467, 824, 3524, 3623, 4206
HphI	GGTGA	8 cut(s)	619, 1868, 2078, 2089, 2330, 2939, 3819, 3992
Hpy99I	CGWCG	2 cut(s)	3890, 4085
HpyCH4IV	ACGT	4 cut(s)	270, 1538, 1630, 4083
HpySE526I	ACGT	4 cut(s)	270, 1538, 1630, 4083
Hsp92I	GRCGYC	1 cut(s)	3521
HspAI	GCGC	5 cut(s)	1418, 3183, 3521, 4312, 4408
KasI	GGCGCC	1 cut(s)	3520
KroI	GCCGGC	1 cut(s)	3523
KroNI	GCCGGC	1 cut(s)	3525
Ksp22I	TGATCA	3 cut(s)	30, 1242, 4183
LguI	GCTCTTC	1 cut(s)	3349
LmnI	GCTCC	5 cut(s)	190, 236, 1138, 2335, 2866
MaeII	ACGT	4 cut(s)	270, 1538, 1630, 4083
MbiI	CCGCTC	1 cut(s)	3435
MfeI	CAATTG	1 cut(s)	219
MflI	RGATCY	7 cut(s)	457, 1199, 1967, 2050, 2920, 3128, 3494
MlsI	TGGCCA	2 cut(s)	3209, 3562
MluNI	TGGCCA	2 cut(s)	3209, 3562
Mly113I	GGCGCC	1 cut(s)	3521
MlyI	GAGTC	3 cut(s)	312, 586, 4220
MmeI	TCCRAC	7 cut(s)	630, 893, 1390, 2912, 3273, 3472, 3658
Mox20I	TGGCCA	2 cut(s)	3209, 3562
Mph1103I	ATGCAT	2 cut(s)	2596, 3718
MroNI	GCCGGC	1 cut(s)	3523
MroXI	GAANNNNTTC	1 cut(s)	2652
MscI	TGGCCA	2 cut(s)	3209, 3562
MslI	CAYNNNNRTG	3 cut(s)	911, 2735, 2966
Msp20I	TGGCCA	2 cut(s)	3209, 3562
MspA1I	CMGCKG	3 cut(s)	961, 4369, 4421
MspI	CCGG	9 cut(s)	19, 52, 177, 283, 467, 824, 3524, 3623, 4206
MunI	CAATTG	1 cut(s)	219
Mva1269I	GAATGC	2 cut(s)	1212, 1615
MvnI	CGCG	6 cut(s)	1950, 2940, 3183, 4119, 4314, 4408
NaeI	GCCGGC	1 cut(s)	3525
NarI	GGCGCC	1 cut(s)	3521
NciI	CCSGG	2 cut(s)	52, 468
NcoI	CCATGG	3 cut(s)	728, 1326, 3228
NgoMIV	GCCGGC	1 cut(s)	3523
NlaIV	GGNNCC	8 cut(s)	459, 822, 1409, 2326, 3496, 3522, 3847, 4290
NmuCI	GTSAC	6 cut(s)	915, 1471, 2248, 2770, 2927, 3825
NruI	TCGCGA	1 cut(s)	4119
NsiI	ATGCAT	2 cut(s)	2596, 3718
NspI	RCATGY	5 cut(s)	151, 557, 699, 2402, 2965
NspV	TTCGAA	1 cut(s)	1977
OliI	CACNNNNGTG	1 cut(s)	911
PagI	TCATGA	4 cut(s)	1027, 1218, 1305, 4506
PaqCI	CACCTGC	1 cut(s)	1531
PceI	AGGCCT	1 cut(s)	3610
PciI	ACATGT	4 cut(s)	553, 695, 2398, 2961
PciSI	GCTCTTC	1 cut(s)	3349
PctI	GAATGC	2 cut(s)	1212, 1615
PdiI	GCCGGC	1 cut(s)	3525
PdmI	GAANNNNTTC	1 cut(s)	2652
PflMI	CCANNNNNTGG	1 cut(s)	729
PfoI	TCCNGGA	1 cut(s)	4215
PleI	GAGTC	3 cut(s)	311, 586, 4220
PluTI	GGCGCC	1 cut(s)	3524
PpsI	GAGTC	3 cut(s)	311, 586, 4220
Ppu21I	YACGTR	1 cut(s)	271
PpuMI	RGGWCCY	2 cut(s)	4213, 4289
PscI	ACATGT	4 cut(s)	553, 695, 2398, 2961
PshBI	ATTAAT	2 cut(s)	681, 3711
Psp124BI	GAGCTC	3 cut(s)	2340, 2444, 2863
Psp1406I	AACGTT	1 cut(s)	1630
Psp5II	RGGWCCY	2 cut(s)	4213, 4289
PspFI	CCCAGC	1 cut(s)	4410
PspN4I	GGNNCC	8 cut(s)	459, 822, 1409, 2326, 3496, 3522, 3847, 4290
PspPI	GGNCC	8 cut(s)	4, 720, 821, 3036, 3225, 4213, 4289, 4413
PspPPI	RGGWCCY	2 cut(s)	4213, 4289
PsrI	GAACNNNNNNTAC	4 cut(s)	1143, 1175, 1896, 1928
PstI	CTGCAG	1 cut(s)	208
PstNI	CAGNNNCTG	4 cut(s)	418, 2368, 3653, 4216
PsuI	RGATCY	7 cut(s)	457, 1199, 1967, 2050, 2920, 3128, 3494
PvuII	CAGCTG	2 cut(s)	4369, 4421
RruI	TCGCGA	1 cut(s)	4119
RsaI	GTAC	8 cut(s)	379, 1671, 1724, 1947, 2353, 2526, 2585, 4307
RsaNI	GTAC	8 cut(s)	378, 1670, 1723, 1946, 2352, 2525, 2584, 4306
RseI	CAYNNNNRTG	3 cut(s)	911, 2735, 2966
SacI	GAGCTC	3 cut(s)	2340, 2444, 2863
SalI	GTCGAC	1 cut(s)	4079
SapI	GCTCTTC	1 cut(s)	3349
Sau96I	GGNCC	8 cut(s)	4, 720, 821, 3036, 3225, 4213, 4289, 4413
ScaI	AGTACT	1 cut(s)	4307
SchI	GAGTC	3 cut(s)	312, 586, 4220
SfcI	CTRYAG	5 cut(s)	204, 751, 1149, 1277, 1902
SfoI	GGCGCC	1 cut(s)	3522
SfuI	TTCGAA	1 cut(s)	1977
SinI	GGWCC	4 cut(s)	821, 3225, 4213, 4289
SmiMI	CAYNNNNRTG	3 cut(s)	911, 2735, 2966
SmlI	CTYRAG	1 cut(s)	2274
SmoI	CTYRAG	1 cut(s)	2274
SnaBI	TACGTA	1 cut(s)	271
SseBI	AGGCCT	1 cut(s)	3610
SspDI	GGCGCC	1 cut(s)	3520
SspI	AATATT	1 cut(s)	2083
SstI	GAGCTC	3 cut(s)	2340, 2444, 2863
StuI	AGGCCT	1 cut(s)	3610
StyI	CCWWGG	5 cut(s)	728, 757, 1326, 3228, 4601
TaiI	ACGT	4 cut(s)	273, 1541, 1633, 4086
TaqII	GACCGA	2 cut(s)	2027, 3941
TatI	WGTACW	1 cut(s)	4305
TauI	GCSGC	3 cut(s)	2091, 4317, 4418
TseFI	GTSAC	6 cut(s)	915, 1471, 2248, 2770, 2927, 3825
Tsp45I	GTSAC	6 cut(s)	915, 1471, 2248, 2770, 2927, 3825
TspGWI	ACGGA	7 cut(s)	995, 1400, 2512, 2911, 3366, 3648, 3806
Van91I	CCANNNNNTGG	1 cut(s)	729
VneI	GTGCAC	1 cut(s)	4135
VpaK11BI	GGWCC	4 cut(s)	821, 3225, 4213, 4289
VspI	ATTAAT	2 cut(s)	681, 3711
XagI	CCTNNNNNAGG	2 cut(s)	130, 4605
XbaI	TCTAGA	3 cut(s)	2029, 2173, 3907
XceI	RCATGY	5 cut(s)	151, 557, 699, 2402, 2965
XcmI	CCANNNNNNNNNTGG	1 cut(s)	419
XmaJI	CCTAGG	1 cut(s)	4601
XmiI	GTMKAC	2 cut(s)	3394, 4080
XmnI	GAANNNNTTC	1 cut(s)	2652
ZrmI	AGTACT	1 cut(s)	4307
Zsp2I	ATGCAT	2 cut(s)	2596, 3718

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.