Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0001783

Physical Location & Seq

Genomic Coordinates Forward (+)

11445107 .. 11456564

Gene Structure 11458 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G010450.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4860 bp

ATGGGTTTTAAGCCAATAGACTGTTATTGTCAACCAGAAGCTAATGGGGTATGGGCCAAAGCAGTCAGTGCTTTTGCCTCATACACTCCTTGTGCAATGGACTCTATGGTCATCTGCATTTCTCATTTAGTCCTTTTTGGACTGTGCTGCTTTCGAATATGGATGATCAAGAAGAATTTGAAATCCCGGAGGTTTAGGTTGAGGTCAAATTACTATAACTACTTGTTGGGTTTGTTGGCTGGTTACTCCACTGCTGAGCCTCTACTGAGGTTGGTGATGGGTTTATCACTTTTTAACCCGTTTGGAAAAACTGGCTTTGCTCCTTTTGAGATGACTTCTTCAATCATTGAAGCTCTTTCTTGGTGCTCTATGCTAATTTTAATTGGCTTGGAAACTAGAATTTACATTCGGGAATTCCGATGGTACGTAAGGTTTGGGGTCCTTTACGTCTTGGTAGGAGATGTTGTGCTGCTAAATCTTATTCTTGGAGTGACAGATTCCTACAGCAGATCTGCTCTGTATCTGTATATAAGCACTGTCTGTTGCCAGGTCTTGTTTGGAATTCTACTTCTCTTCTATGTCCCCAATTTGGATCCTTATCCCGGCTATGTTGTTCTGCAATCCGAGTCACTTGACAATGCTGAATATGAAGCACTACAAGGAGAAGATCAAATTTGCCCTGAACGGCATGTTAATATATTTTCCAGAATATATTTTGGATGGATGACTCCACTTATGCAGCTAGGGTACAGAAAACCCATCACTGAAGCAGATGTTTGGAAGTTGGACACATGGGATCAGACCGAGACATTGATTAAGAGGTTTCAGGAATGTTGGGTTGAAGAATCAAAAAGGTCCAAGCCATGGCTTCTGAGAGCATTGAATTGTAGCCTTGGACGAAGGTTCTGGTTGGGAGGCTTTTTCAAGATTGGCAATGATCTCTCCCAGTTTTCGGGTCCCATTCTATTAAATCATCTCTTGCAGTCAATGCAACGAGGTGATCCAGCTTGGATGGGTTACATATATGCCTTCTTAATTTTCATTGGTGTGTCATTTGGTGTGCTCTCTGAATCTCAGTATTTCCAGAATGTTATGAGGGTCGGCTTTCGGTTGAGATCAACTTTGGTAGCTGCTATATTCCGTAAATCTATAAGAATAACGCATGAGGGCCGCAAGAATTTTCCAACTGGGAAGATAACGAATATGATGTCTACAGATGCTAATGCACTCCAGCAAATATGCCAACAACTTCACGGACTATGGTCTGCTCCTTTCCGTATCATTGTAGCTATGACTCTTCTCTACCAACAATTAGGTGTTGCATCGCTTATTGGATCCTTGATGCTAGTTCTCATGATCCCCATACAGACAACTATCATCAGCAAAATGCGGAAACTGACAAAGGATGGACTGCAACAGACTGACAAGAGAGTTGGCCTCATGAATGAAATTTTAGCTGCCATGGACACTGTGAAATGTTATGCCTGGGAGACAAGTTTCCAGCAGCGAGTTCAAAGTATACGGAATGATGAGCTCTCAAGGTTCCGTAAAGCACAATTACTATCTGCTTTGAACAGTTTTATTCTCAACAGCATCCCAGTTGTTGTGACAGTTACTTCATTTGGAGTATTCACTTTTCTTGGAGGTGAATTGACACCTGCTAGGGCGTTTACATCACTTTCTCTTTTTGCAGTGCTACGCTTTCCTTTAAACATGCTGCCCAACTTGTTAAGTCAGGTTGTAAATGCAAACGTATCCTTGCAGCGTTTGGAGGAACTATTTTTAACTGAGGAGAGAATTCTAGTACCCAACCCGCCACTGGAACCAGGGCTTCCAGCCATCTCAATTCAGGATGGGTACTTTTCATGGGATTCAAAGGCAGAGAAACCCACATTATCAAACATCAACTTAGATATACCAGTTGGAAGATTAGTTGCAGTTGTTGGGGGCACTGGAGAAGGAAAGACATCACTTATATCAGCCATGCTTGGGGAGCTGCCTCCCATTGCAGGTGCCAGTGTTGTAATCAGGGGGACTGTTGCTTATGTTCCCCAAGTTTCATGGATTTTCAATGCTACTGTACGTGAAAACATTTTATTTGGATCTGAGTTTGAAGCTGCACGATATTGGAAGGCTATTGATGTGACTGAGTTCCATCATGACCTAGACCTACTTCCAGGTCGAGATCTTACAGAGATAGGTGAAAGAGGGGTCAATATTAGTGGAGGTCAAAAGCAAAGGGTTTCTATGGCTCGAGCTGTATATTCTAATTCAGATGTTTACATATTTGATGACCCATTAAGTGCTTTAGATGCTCATGTTGCTAGAGAGGTTTTCAACCACTGTATCAAGGAAGAGTTGCAGGGGAAAACTAGGGTGCTTGTCACGAATCAGCTACACTTTCTGCCTCAAGTGGATGAAATTATATTAGTCTCTGATGGTACGATTAAAGAGAAGGGAACCTTTAAGGATCTCTCTGAAAACAGTTTGCTGTTCCAGAAGCTAATGGAAAATGCCGGAAAAATGGAAGAACATGTAGAAGAACAGGAAGCCAGCAAAACTGATAAACAGGAAACCTCACTTCCTGTGTCTAACGGGGTGGCAAATGACTTGCCAAAAGATACAAACTACACAAAGAAAGGGAAAGGGATGAGATCTGTACTCATTAGACAAGAGGAACGGGAAACAGGTGTTGTAAGTTGGAAAATTTTGCAGAGGTATAAGCATGCATTAGGAGGCCTATGGGTGGTCATGGTTCTATTTACTTGTTATACACTAACAGAAGTTCTTCGAGTTTCAAGCAGCACATGGTTAAGTTTTTGGACAGACCAAAGCACATCAAAGAGTTATGCACCTGGATTCTACATTCTTATATATGCAATTCTGTCTATTGGTCAGGTAACGGTGACTCTGACGAACTCTTTTTGGCTTATCACTTCAAGTCTTCGTGCAGCCAGAAAATTGCATGATGCCCTGCTACATGCTATACTAAAAGCTCCAATGGTATTCTTTCACACTAACCCAATTGGAAGGATAATCAATAGGTTTGCAAAGGATCTTGGTGATATAGATCGCAATGTAGCCAACTTTATGAACATGTTTCTGGGTCAAGTGTGGCAGCTCCTTTCAACTTTTGTGCTCATAGGCATTGTGAGCACAATATCCCTCTGGGCCATAATGCCACTTCTAATTTTGTTCTATGCAGCCTATTTATTCTATCAGAGCACATCCCGTGAAGTGAAACGCATGGATTCCATTACCAGATCTCCAGTTTATGCACAGTTTGGAGAAGCATTAAATGGTTTGTCATCAATTCGTGCCTATAAAGCTTATGATCGGATGGCAAACATCAGTGGAAGGTCTATGGATAATAACATCAGATTCACCCTTGTGAATATTAGTTCAAACCGCTGGCTTACAATAAGGTTGGAAACACTAGGAGGTATCATGATCTGGGTGATTGCAACCTTTGCTGTCATGCAAAATGGAAGAGCAGAAAACCAAGTACAATTTGCATCTACAATGGGTCTGCTTCTCACTTATACTTTGAATATCACTAGTCTGTTGAGTGGTGTTCTTAGACAAGCTAGCAGGGCTGAAAATAGTTTAAATGCCGTTGAGCGGGTTGGATCATATATAGAATTGCCTTCTGAGGCTCCAGCTGTTATTGAAAGCAACCGTCCTCCACATGGGTGGCCATCATCAGGATCAATCAAGTTTGAGGATGTTGTCCTCCGTTATAGGCCTGGACTTCCACCTGTACTGCATGGATTATCGTTTACAGTTTCTGCAAGTGAGAAGCTAGGAATAGTTGGAAGAACTGGTGCTGGCAAATCTAGCATGATTAATGCATTGTTTAGGATAGTAGAAATAGAAAAAGGAAGCATTTTGATTGATGGTTGTAATGTTTCTAAGTTTGGACTGACAGATTTGAGAAAAGTTCTTAGCATCATACCACAATCACCGGTTCTTTTTTCAGGAACTGTGCGGTTTAATCTTGATCCTTTCAGTGAGCATAATGATGCTGACCTTTGGGAAGCCCTGGAAAGGGCACATCTAAAGGATGTCATTAGGAGGAATTCCTTCGGTCTCGATGCTGAGGTTTCCGAGGGTGGTGAGAATTTCAGTGTTGGACAGAGGCAGCTAATTAGTCTAGCTAGAGCATTGCTACGGAGATCAAAGATTCTTGTTCTTGATGAAGCGACTGCAGCTGTGGATGTTAGAACTGATGCTCTCATCCAAAAGACCATACGTGAAGAATTCAAATCATGCACCATGCTCGTTATTGCTCACAGATTGAACACCATTATCGACTGTGATCGAATTCTTGTTCTTGATGCTGGTCAGGTCCTAGAACACGGTTCCCCAGAAGAACTACTGCTAAATGAAGAGAGCGCATTCTCCAAGATGGTCAGGAGTACAGGCCCTTCTAATGCGCAGTACTTACGCAGCCTGGTATTTGAAGGCAAGCAGAATAAGGTGAATAGAGACGAAGCTGAGCAACTGTTTGGCCAGAGCCAGAGGAGATGGTTAGCCTCTTCGCGCTGGGCAGCTGCTGCCCAATTTGCATTGGCTGTCAGTCTCACATCCTCACAGAACGATCTCCAGAGGTTAGATATTGGAGACGAGGACAATATCCTCATGAAAACAAAGGATGCAGTTATAACACTACAGGGAGTTTTGGAAGGAAAGCATGATGAAGATATAGACATATCACTAAATCAGCACCGTATTCCAAGGGAGGGGTGGTGGTCAGCTTTCTTCAGAATAGTTGAAGGTCTGGCCGTGATGGGCAGGCTGGCTCAGAACAGACTCCACCCTTTAGAAGATGACGATTTTGAAGACCAGTCTTTATACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006810 GO:0008150 GO:0015399 GO:0015405 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022804 GO:0022857 GO:0031090 GO:0042623 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko01523 ko01524 ko02010 ko04976 map01523 map01524 map02010 map04976

Pfam Domains

Protein Families

ABC_membrane ABC_tran

Protein Analysis

1619

Amino Acids

182.02

Weight (kDa)

8.07

Isoelectric Point (pI)

42.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ABC_membrane	PF00664	303 - 570	4.5e-39	ABC transporter transmembrane region
ABC_tran	PF00005	632 - 767	7.5e-19	ABC transporter
ABC_membrane	PF00664	918 - 1179	1.9e-29	ABC transporter transmembrane region
ABC_tran	PF00005	1255 - 1403	2.5e-26	ABC transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001783)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30410 AT1G30410 AT1G30410 AT1G30410 AT1G30410 AT1G30410 AT1G30420 AT1G30420
fragaria_vesca	FvH4_2g14981 FvH4_2g14981 FvH4_2g14981 FvH4_2g14981 FvH4_2g14981 FvH4_2g14981 FvH4_2g14981 FvH4_3g09710 FvH4_3g09720
malus_domestica	MD10G1255300.v1.1 MD10G1255500.v1.1
prunus_persica	Prupe.4G088600_v2.0.a1 Prupe.4G088600_v2.0.a1
pyrus_communis	pycom10g21050
rosa_chinensis	RchiOBHm_Chr3g0492011 RchiOBHm_Chr5g0015441 RchiOBHm_Chr5g0015451 RchiOBHm_Chr5g0015481 RchiOBHm_Chr5g0015491
rosa_laevigata	RLG00000032199 RLG00000032201 RLG00000032202
rosa_multiflora	Rmu_sc0019960.1_g000002
rosa_roxburghii	Rroxscaffold_1G00061020 Rroxscaffold_1G00061040 Rroxscaffold_1G00061050 Rroxscaffold_6G00392640
rosa_rugosa	Rorug03G0251100 Rorug05G0027400 Rorug05G0027500 Rorug05G0027700
rosa_samantha	Rh3AG301500 Rh5AG121000 Rh5AG121100 Rh5CG130600 Rh5DG118900 Rh5DG119000
rosa_wichuraiana	Rw5G010450 Rw5G010470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	4664
AarI	CACCTGC	2 cut(s)	1666, 2000
AasI	GACNNNNNNGTC	1 cut(s)	107
Acc16I	TGCGCA	1 cut(s)	4437
Acc36I	ACCTGC	2 cut(s)	1666, 2000
AccB1I	GGYRCC	1 cut(s)	2012
AccB7I	CCANNNNNTGG	1 cut(s)	864
AccBSI	CCGCTC	1 cut(s)	3622
AccI	GTMKAC	2 cut(s)	1211, 1519
AccII	CGCG	1 cut(s)	4543
AciI	CCGC	6 cut(s)	1171, 1390, 1814, 3409, 3622, 3988
AcoI	YGGCCR	3 cut(s)	3695, 4510, 4782
AcuI	CTGAAG	2 cut(s)	786, 4747
AflIII	ACRYGT	2 cut(s)	2533, 3096
AgeI	ACCGGT	1 cut(s)	3964
AhlI	ACTAGT	1 cut(s)	3557
AjnI	CCWGG	8 cut(s)	546, 1484, 1825, 2176, 2854, 3745, 4041, 4452
AleI	CACNNNNGTG	2 cut(s)	3389, 3691
Alw21I	GWGCWC	6 cut(s)	368, 1065, 1536, 3141, 3158, 3227
Alw26I	GTCTC	7 cut(s)	800, 1484, 2437, 4094, 4482, 4586, 4617
AlwNI	CAGNNNCTG	3 cut(s)	3788, 3983, 4556
Ama87I	CYCGRG	1 cut(s)	2253
ArsI	GACNNNNNNTTYG	2 cut(s)	1193, 1225
AseI	ATTAAT	1 cut(s)	3846
AsiGI	ACCGGT	1 cut(s)	3964
Asp700I	GAANNNNTTC	2 cut(s)	2787, 4082
AspLEI	GCGC	3 cut(s)	4397, 4438, 4545
AspS9I	GGNCC	8 cut(s)	54, 439, 855, 956, 1168, 3171, 4348, 4424
AsuC2I	CCSGG	2 cut(s)	187, 603
AsuII	TTCGAA	1 cut(s)	154
AsuNHI	GCTAGC	1 cut(s)	3587
AvaI	CYCGRG	1 cut(s)	2253
AvaII	GGWCC	4 cut(s)	439, 855, 956, 4348
BaeGI	GKGCMC	2 cut(s)	1952, 4054
BaeI	ACNNNNGTAYC	2 cut(s)	2329, 2362
BalI	TGGCCA	2 cut(s)	3697, 4512
BamHI	GGATCC	2 cut(s)	592, 1334
BanI	GGYRCC	1 cut(s)	2012
BanII	GRGCYC	1 cut(s)	1536
BarI	GAAGNNNNNNTAC	2 cut(s)	4411, 4443
BbsI	GAAGAC	2 cut(s)	2936, 4848
Bbv12I	GWGCWC	6 cut(s)	368, 1065, 1536, 3141, 3158, 3227
BbvCI	CCTCAGC	1 cut(s)	4098
BceAI	ACGGC	3 cut(s)	701, 3599, 4769
BcgI	CGANNNNNNTGC	2 cut(s)	4261, 4295
BciT130I	CCWGG	8 cut(s)	548, 1486, 1827, 2178, 2856, 3747, 4043, 4454
BciVI	GTATCC	1 cut(s)	1765
BclI	TGATCA	1 cut(s)	165
BcnI	CCSGG	2 cut(s)	187, 603
BcoDI	GTCTC	7 cut(s)	800, 1484, 2437, 4094, 4482, 4586, 4617
BcuI	ACTAGT	1 cut(s)	3557
BfmI	CTRYAG	4 cut(s)	502, 1212, 4206, 4670
BfuAI	ACCTGC	2 cut(s)	1666, 2000
BfuI	GTATCC	1 cut(s)	1765
BglII	AGATCT	4 cut(s)	509, 2185, 2654, 3263
BlpI	GCTNAGC	2 cut(s)	255, 4497
BmcAI	AGTACT	1 cut(s)	4442
Bme18I	GGWCC	4 cut(s)	439, 855, 956, 4348
BmeT110I	CYCGRG	1 cut(s)	2253
BmgT120I	GGNCC	8 cut(s)	54, 439, 855, 956, 1168, 3171, 4348, 4424
BmrI	ACTGGG	3 cut(s)	940, 1197, 1592
BmtI	GCTAGC	1 cut(s)	3591
BmuI	ACTGGG	3 cut(s)	940, 1197, 1592
BoxI	GACNNNNGTC	1 cut(s)	1261
BpiI	GAAGAC	2 cut(s)	2936, 4848
BplI	GAGNNNNNCTC	2 cut(s)	1422, 1454
BpmI	CTGGAG	5 cut(s)	1214, 1974, 3252, 3642, 4589
Bpu10I	CCTNAGC	1 cut(s)	4098
Bpu1102I	GCTNAGC	2 cut(s)	255, 4497
Bpu14I	TTCGAA	1 cut(s)	154
BpuEI	CTTGAG	2 cut(s)	1522, 2394
BpuMI	CCSGG	2 cut(s)	187, 603
BsaAI	YACGTR	3 cut(s)	427, 2084, 4253
BsaBI	GATNNNNATC	2 cut(s)	597, 3069
BsaI	GGTCTC	1 cut(s)	4094
BsaJI	CCNNGG	8 cut(s)	863, 892, 1461, 1485, 1826, 4041, 4107, 4736
BsaWI	WCCGGW	1 cut(s)	3964
BsaXI	ACNNNNNCTCC	6 cut(s)	450, 480, 3250, 3280, 4733, 4763
Bse118I	RCCGGY	1 cut(s)	3964
Bse3DI	GCAATG	5 cut(s)	102, 940, 2004, 3082, 4163
Bse8I	GATNNNNATC	2 cut(s)	597, 3069
BseBI	CCWGG	8 cut(s)	548, 1486, 1827, 2178, 2856, 3747, 4043, 4454
BseDI	CCNNGG	8 cut(s)	863, 892, 1461, 1485, 1826, 4041, 4107, 4736
BseJI	GATNNNNATC	2 cut(s)	597, 3069
BseMI	GCAATG	5 cut(s)	102, 940, 2004, 3082, 4163
BseRI	GAGGAG	2 cut(s)	1805, 4537
BseSI	GKGCMC	2 cut(s)	1952, 4054
BseYI	CCCAGC	1 cut(s)	4545
BsgI	GTGCAG	2 cut(s)	2103, 2970
Bsh1236I	CGCG	1 cut(s)	4543
BshNI	GGYRCC	1 cut(s)	2012
BshTI	ACCGGT	1 cut(s)	3964
BsiHKAI	GWGCWC	6 cut(s)	368, 1065, 1536, 3141, 3158, 3227
BsiHKCI	CYCGRG	1 cut(s)	2253
BsiSI	CCGG	4 cut(s)	187, 603, 2517, 3965
BslFI	GGGAC	3 cut(s)	566, 942, 2047
BsmAI	GTCTC	7 cut(s)	800, 1484, 2437, 4094, 4482, 4586, 4617
BsmBI	CGTCTC	2 cut(s)	4482, 4617
BsmFI	GGGAC	3 cut(s)	566, 942, 2047
BsmI	GAATGC	1 cut(s)	4397
Bso31I	GGTCTC	1 cut(s)	4094
BsoBI	CYCGRG	1 cut(s)	2253
Bsp119I	TTCGAA	1 cut(s)	154
Bsp1286I	GDGCHC	8 cut(s)	368, 1065, 1536, 1952, 3141, 3158, 3227, 4054
Bsp1720I	GCTNAGC	2 cut(s)	255, 4497
Bsp19I	CCATGG	2 cut(s)	863, 1461
BspACI	CCGC	6 cut(s)	1171, 1390, 1814, 3409, 3622, 3988
BspFNI	CGCG	1 cut(s)	4543
BspHI	TCATGA	5 cut(s)	1353, 1440, 2158, 3447, 4641
BspMAI	CTGCAG	1 cut(s)	4210
BspMI	ACCTGC	2 cut(s)	1666, 2000
BspOI	GCTAGC	1 cut(s)	3591
BspQI	GCTCTTC	1 cut(s)	3484
BspT104I	TTCGAA	1 cut(s)	154
BspT107I	GGYRCC	1 cut(s)	2012
BspTNI	GGTCTC	1 cut(s)	4094
BsrBI	CCGCTC	1 cut(s)	3622
BsrDI	GCAATG	5 cut(s)	102, 940, 2004, 3082, 4163
BsrFI	RCCGGY	1 cut(s)	3964
BssAI	RCCGGY	1 cut(s)	3964
BssECI	CCNNGG	8 cut(s)	863, 892, 1461, 1485, 1826, 4041, 4107, 4736
BssNAI	GTATAC	1 cut(s)	1520
BssT1I	CCWWGG	4 cut(s)	863, 892, 1461, 4736
Bst1107I	GTATAC	1 cut(s)	1520
Bst2UI	CCWGG	8 cut(s)	548, 1486, 1827, 2178, 2856, 3747, 4043, 4454
Bst6I	CTCTTC	6 cut(s)	578, 1302, 2349, 3484, 4383, 4543
BstAPI	GCANNNNNTGC	4 cut(s)	68, 988, 3470, 4556
BstBAI	YACGTR	3 cut(s)	427, 2084, 4253
BstBI	TTCGAA	1 cut(s)	154
BstC8I	GCNNGC	8 cut(s)	2554, 2727, 3413, 3589, 3829, 4469, 4796, 4800
BstDSI	CCRYGG	2 cut(s)	863, 1461
BstFNI	CGCG	1 cut(s)	4543
BstHHI	GCGC	3 cut(s)	4397, 4438, 4545
BstMAI	GTCTC	7 cut(s)	800, 1484, 2437, 4094, 4482, 4586, 4617
BstNI	CCWGG	8 cut(s)	548, 1486, 1827, 2178, 2856, 3747, 4043, 4454
BstNSI	RCATGY	6 cut(s)	692, 1717, 2537, 2729, 2984, 3100
BstPAI	GACNNNNGTC	1 cut(s)	1261
BstSFI	CTRYAG	4 cut(s)	502, 1212, 4206, 4670
BstSLI	GKGCMC	2 cut(s)	1952, 4054
BstSNI	TACGTA	1 cut(s)	427
BstUI	CGCG	1 cut(s)	4543
BstV2I	GAAGAC	2 cut(s)	2936, 4848
BstX2I	RGATCY	9 cut(s)	509, 592, 1334, 2102, 2185, 2470, 2654, 3055, 3263
BstXI	CCANNNNNNTGG	1 cut(s)	3693
BstYI	RGATCY	9 cut(s)	509, 592, 1334, 2102, 2185, 2470, 2654, 3055, 3263
BstZ17I	GTATAC	1 cut(s)	1520
BsuI	GTATCC	1 cut(s)	1765
BtgI	CCRYGG	2 cut(s)	863, 1461
BtgZI	GCGATG	1 cut(s)	1308
BtsI	GCAGTG	2 cut(s)	249, 1698
BveI	ACCTGC	2 cut(s)	1666, 2000
Cac8I	GCNNGC	8 cut(s)	2554, 2727, 3413, 3589, 3829, 4469, 4796, 4800
CaiI	CAGNNNCTG	3 cut(s)	3788, 3983, 4556
CciI	TCATGA	5 cut(s)	1353, 1440, 2158, 3447, 4641
CfoI	GCGC	3 cut(s)	4397, 4438, 4545
Cfr10I	RCCGGY	1 cut(s)	3964
Cfr13I	GGNCC	8 cut(s)	54, 439, 855, 956, 1168, 3171, 4348, 4424
CspAI	ACCGGT	1 cut(s)	3964
CspCI	CAANNNNNGTGG	2 cut(s)	2203, 2238
DraI	TTTAAA	2 cut(s)	1710, 3609
DrdI	GACNNNNNNGTC	1 cut(s)	107
DseDI	GACNNNNNNGTC	1 cut(s)	107
EaeI	YGGCCR	3 cut(s)	3695, 4510, 4782
Eam1104I	CTCTTC	6 cut(s)	578, 1302, 2349, 3484, 4383, 4543
EarI	CTCTTC	6 cut(s)	578, 1302, 2349, 3484, 4383, 4543
Ecl136II	GAGCTC	1 cut(s)	1534
Eco105I	TACGTA	1 cut(s)	427
Eco130I	CCWWGG	4 cut(s)	863, 892, 1461, 4736
Eco147I	AGGCCT	2 cut(s)	2739, 3745
Eco24I	GRGCYC	1 cut(s)	1536
Eco31I	GGTCTC	1 cut(s)	4094
Eco47I	GGWCC	4 cut(s)	439, 855, 956, 4348
Eco53kI	GAGCTC	1 cut(s)	1534
Eco57I	CTGAAG	2 cut(s)	786, 4747
Eco88I	CYCGRG	1 cut(s)	2253
EcoICRI	GAGCTC	1 cut(s)	1534
EcoO109I	RGGNCCY	4 cut(s)	439, 956, 4348, 4424
EcoRI	GAATTC	6 cut(s)	413, 561, 1797, 4078, 4259, 4323
EcoRII	CCWGG	8 cut(s)	546, 1484, 1825, 2176, 2854, 3745, 4041, 4452
EcoT14I	CCWWGG	4 cut(s)	863, 892, 1461, 4736
EcoT22I	ATGCAT	2 cut(s)	2731, 3853
EcoT38I	GRGCYC	1 cut(s)	1536
ErhI	CCWWGG	4 cut(s)	863, 892, 1461, 4736
Esp3I	CGTCTC	2 cut(s)	4482, 4617
FalI	AAGNNNNNCTT	2 cut(s)	2447, 2479
FaqI	GGGAC	3 cut(s)	566, 942, 2047
FauI	CCCGC	2 cut(s)	1821, 3615
FbaI	TGATCA	1 cut(s)	165
FblI	GTMKAC	2 cut(s)	1211, 1519
FriOI	GRGCYC	1 cut(s)	1536
FspI	TGCGCA	1 cut(s)	4437
GlaI	GCGC	3 cut(s)	4396, 4437, 4544
GsaI	CCCAGC	1 cut(s)	4549
GsuI	CTGGAG	5 cut(s)	1214, 1974, 3252, 3642, 4589
HapII	CCGG	4 cut(s)	187, 603, 2517, 3965
HhaI	GCGC	3 cut(s)	4397, 4438, 4545
Hin6I	GCGC	3 cut(s)	4395, 4436, 4543
HinP1I	GCGC	3 cut(s)	4395, 4436, 4543
HincII	GTYRAC	1 cut(s)	32
HindII	GTYRAC	1 cut(s)	32
HindIII	AAGCTT	1 cut(s)	3327
HpaII	CCGG	4 cut(s)	187, 603, 2517, 3965
Hpy166II	GTNNAC	6 cut(s)	32, 1212, 1520, 1671, 2281, 3780
Hpy8I	GTNNAC	6 cut(s)	32, 1212, 1520, 1671, 2281, 3780
HpyCH4IV	ACGT	5 cut(s)	426, 447, 1752, 2083, 4252
HpySE526I	ACGT	5 cut(s)	426, 447, 1752, 2083, 4252
HspAI	GCGC	3 cut(s)	4395, 4436, 4543
KflI	GGGWCCC	1 cut(s)	956
Ksp22I	TGATCA	1 cut(s)	165
LguI	GCTCTTC	1 cut(s)	3484
LmnI	GCTCC	6 cut(s)	325, 1273, 1993, 3001, 3126, 3661
MaeII	ACGT	5 cut(s)	426, 447, 1752, 2083, 4252
MbiI	CCGCTC	1 cut(s)	3622
MfeI	CAATTG	1 cut(s)	3024
MflI	RGATCY	9 cut(s)	509, 592, 1334, 2102, 2185, 2470, 2654, 3055, 3263
MhlI	GDGCHC	8 cut(s)	368, 1065, 1536, 1952, 3141, 3158, 3227, 4054
MlsI	TGGCCA	2 cut(s)	3697, 4512
MluNI	TGGCCA	2 cut(s)	3697, 4512
MlyI	GAGTC	6 cut(s)	95, 635, 721, 1288, 2902, 4806
MmeI	TCCRAC	8 cut(s)	765, 1208, 1903, 2681, 3408, 3607, 3793, 4111
Mox20I	TGGCCA	2 cut(s)	3697, 4512
Mph1103I	ATGCAT	2 cut(s)	2731, 3853
MroXI	GAANNNNTTC	2 cut(s)	2787, 4082
MscI	TGGCCA	2 cut(s)	3697, 4512
MslI	CAYNNNNRTG	4 cut(s)	1046, 3389, 3691, 4020
Msp20I	TGGCCA	2 cut(s)	3697, 4512
MspA1I	CMGCKG	4 cut(s)	3411, 3662, 4211, 4553
MspI	CCGG	4 cut(s)	187, 603, 2517, 3965
MunI	CAATTG	1 cut(s)	3024
Mva1269I	GAATGC	1 cut(s)	4397
MvaI	CCWGG	8 cut(s)	548, 1486, 1827, 2178, 2856, 3747, 4043, 4454
MvnI	CGCG	1 cut(s)	4543
NciI	CCSGG	2 cut(s)	187, 603
NcoI	CCATGG	2 cut(s)	863, 1461
NheI	GCTAGC	1 cut(s)	3587
NmuCI	GTSAC	6 cut(s)	490, 627, 1606, 2143, 2383, 2905
NsbI	TGCGCA	1 cut(s)	4437
NsiI	ATGCAT	2 cut(s)	2731, 3853
NspI	RCATGY	6 cut(s)	692, 1717, 2537, 2729, 2984, 3100
NspV	TTCGAA	1 cut(s)	154
OliI	CACNNNNGTG	2 cut(s)	3389, 3691
PaeI	GCATGC	1 cut(s)	2729
PaeR7I	CTCGAG	1 cut(s)	2253
PagI	TCATGA	5 cut(s)	1353, 1440, 2158, 3447, 4641
PaqCI	CACCTGC	2 cut(s)	1666, 2000
PceI	AGGCCT	2 cut(s)	2739, 3745
PciI	ACATGT	2 cut(s)	2533, 3096
PciSI	GCTCTTC	1 cut(s)	3484
PcsI	WCGNNNNNNNCGW	1 cut(s)	415
PctI	GAATGC	1 cut(s)	4397
PdmI	GAANNNNTTC	2 cut(s)	2787, 4082
PfeI	GAWTC	9 cut(s)	497, 845, 1070, 1871, 2389, 2859, 3251, 3381, 4183
PflMI	CCANNNNNTGG	1 cut(s)	864
PfoI	TCCNGGA	1 cut(s)	185
PinAI	ACCGGT	1 cut(s)	3964
PleI	GAGTC	6 cut(s)	95, 634, 721, 1288, 2902, 4806
PpsI	GAGTC	6 cut(s)	95, 634, 721, 1288, 2902, 4806
Ppu21I	YACGTR	3 cut(s)	427, 2084, 4253
PpuMI	RGGWCCY	3 cut(s)	439, 956, 4348
PscI	ACATGT	2 cut(s)	2533, 3096
PshAI	GACNNNNGTC	1 cut(s)	1261
PshBI	ATTAAT	1 cut(s)	3846
PsiI	TTATAA	1 cut(s)	4664
Psp124BI	GAGCTC	1 cut(s)	1536
Psp5II	RGGWCCY	3 cut(s)	439, 956, 4348
Psp6I	CCWGG	8 cut(s)	546, 1484, 1825, 2176, 2854, 3745, 4041, 4452
PspFI	CCCAGC	1 cut(s)	4545
PspGI	CCWGG	8 cut(s)	546, 1484, 1825, 2176, 2854, 3745, 4041, 4452
PspPI	GGNCC	8 cut(s)	54, 439, 855, 956, 1168, 3171, 4348, 4424
PspPPI	RGGWCCY	3 cut(s)	439, 956, 4348
PspXI	VCTCGAGB	1 cut(s)	2253
PstI	CTGCAG	1 cut(s)	4210
PstNI	CAGNNNCTG	3 cut(s)	3788, 3983, 4556
PsuI	RGATCY	9 cut(s)	509, 592, 1334, 2102, 2185, 2470, 2654, 3055, 3263
PvuII	CAGCTG	3 cut(s)	3662, 4211, 4553
RseI	CAYNNNNRTG	4 cut(s)	1046, 3389, 3691, 4020
SacI	GAGCTC	1 cut(s)	1536
SapI	GCTCTTC	1 cut(s)	3484
Sau96I	GGNCC	8 cut(s)	54, 439, 855, 956, 1168, 3171, 4348, 4424
ScaI	AGTACT	1 cut(s)	4442
SchI	GAGTC	6 cut(s)	95, 635, 721, 1288, 2902, 4806
SduI	GDGCHC	8 cut(s)	368, 1065, 1536, 1952, 3141, 3158, 3227, 4054
SfcI	CTRYAG	4 cut(s)	502, 1212, 4206, 4670
Sfr274I	CTCGAG	1 cut(s)	2253
SfuI	TTCGAA	1 cut(s)	154
SinI	GGWCC	4 cut(s)	439, 855, 956, 4348
SlaI	CTCGAG	1 cut(s)	2253
SmiMI	CAYNNNNRTG	4 cut(s)	1046, 3389, 3691, 4020
SmlI	CTYRAG	3 cut(s)	1537, 2253, 2409
SmoI	CTYRAG	3 cut(s)	1537, 2253, 2409
SnaBI	TACGTA	1 cut(s)	427
SpeI	ACTAGT	1 cut(s)	3557
SphI	GCATGC	1 cut(s)	2729
SseBI	AGGCCT	2 cut(s)	2739, 3745
SsiI	CCGC	6 cut(s)	1171, 1390, 1814, 3409, 3622, 3988
SspI	AATATT	2 cut(s)	2218, 3397
SstI	GAGCTC	1 cut(s)	1536
StuI	AGGCCT	2 cut(s)	2739, 3745
StyI	CCWWGG	4 cut(s)	863, 892, 1461, 4736
TaiI	ACGT	5 cut(s)	429, 450, 1755, 2086, 4255
TaqI	TCGA	7 cut(s)	154, 2182, 2254, 2791, 4092, 4311, 4321
TaqII	GACCGA	2 cut(s)	818, 4076
TatI	WGTACW	5 cut(s)	2659, 3505, 3760, 4418, 4440
TauI	GCSGC	1 cut(s)	1173
TfiI	GAWTC	9 cut(s)	497, 845, 1070, 1871, 2389, 2859, 3251, 3381, 4183
TseFI	GTSAC	6 cut(s)	490, 627, 1606, 2143, 2383, 2905
Tsp45I	GTSAC	6 cut(s)	490, 627, 1606, 2143, 2383, 2905
TspGWI	ACGGA	7 cut(s)	1130, 1265, 1269, 1535, 1537, 3725, 4186
Van91I	CCANNNNNTGG	1 cut(s)	864
VpaK11BI	GGWCC	4 cut(s)	439, 855, 956, 4348
VspI	ATTAAT	1 cut(s)	3846
XceI	RCATGY	6 cut(s)	692, 1717, 2537, 2729, 2984, 3100
XcmI	CCANNNNNNNNNTGG	3 cut(s)	554, 1816, 4743
XhoI	CTCGAG	1 cut(s)	2253
XmiI	GTMKAC	2 cut(s)	1211, 1519
XmnI	GAANNNNTTC	2 cut(s)	2787, 4082
ZrmI	AGTACT	1 cut(s)	4442
Zsp2I	ATGCAT	2 cut(s)	2731, 3853

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.