Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0001783

Physical Location & Seq

Genomic Coordinates Forward (+)

12010026 .. 12027625

Gene Structure 17600 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000032201

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4857 bp

ATGGGTTTTAAGCCAATAGACTGGTATTGTCAGCCAGAAGCTAATGGGGTATGGGCCAAAGCAGTCAGTGCTTTTGGCTCATACACACCTTGTGCAATGGACTCTATGGTCATCTGCATTTCTCATTTACTTCTTTTCGGTTTGTGCTGCTATCGAATATGGATGATCAACAAGAATTTGAAAGCCGGGAGGTTCAGGTTGAGGTCAAATTACTATAATTACTTGTTGGGATTGTTGGCTGGTTACTCCACTGCTGAGCCTCTACTGAGGTTGGTGATGGGTTTATCACTTTTTAACCCGTCTGGCTTTGCTCCTTTTGAGATGACTTCTTCGATCATTGAAGCTCTTGCTTGGTGCTCTATGCTAATTTTGATTGGCTTGGAAACTAAGATATACATTCGCGAATGCCATTGGTACGTAAGGTTTGGAGTCCTTTATGTCTTGGTAGGAGATGCTGTGGTGCTAAATCTTATTCTTGGAGTGACAGATTTCTACAGTGGGTCTGCTCTGTATCTGTATATAAGCACCGTCTGTTGCCAGGTGTTGTTTGGAATTCTACTTCTCATCTATGTTCCCAATTTGGATCCTTATCCCGGGTACGCTGTTCTGCAATCTGAGTCACCTGACAATGCTGAATATGAAGCACTACCTGGAGAAGATCAAATTTGCCCTGAACGGCATGTTAATATATTCTCCAGATTATATTTTGGATGGATGACTCCACTCATGCAACTAGGGTACAGAAAACCCATCACTGAAACAGATGTTTGGAAGTTGGACACATGGGATCAGACCGAGACATTGATTAAAAGGTTTCTGGAATGCTGGGTTGAAGAATCTCGAAGGTCCAAGCCATGGCTTCTGAGAGCACTGAATTGTAGCCTTGGACGAAGATTCTGGTTGGGAGGTATTTTCAAGCTTGGCAATGATCTCTCTAAGTTTTCGGGTCCCATTCTATTAAATCATCTCTTGCAGTCAATGCAACGAGGTGATCCAGCTTGGATTGGTTATATATATGCGTTCTTAATTTTCATTGGTGTGTCATTTGGTGTGCTATCTGAATCTCAGTATTTCCAGAATGTTATGCGGGTTAGCTTTCGGTTGAGATCAACTTTGGTAGCTGCTATATTCCGTAAATCTATAAGAATAACACATGAGGGCCGTAAGAATTTTCCAACTGGGAAGATAATGAATATGATATCTTCAGATGCTAATGCACTCCAGAAAGCATGCGAACAACTTCACGGACTATGGTCTGCTCCTTTTCGTATCATTGTAGCTATGCTTCTTCTTTACCAACAATTAGGTGTTGCATCGCTTATTGGATCCTGGTCGCTAGTTCTCATGATCCCCATACAGACAACAGTGATCAGCAAAATGCGGAAACTGACGAAGGATGGATTGCAACAGACTGACAAGAGAGTTGGCCTCATGAATGAAATTTTAGCTGCCATGGATACGGTGAAATGTTATGCCTGGGAGACAAGTTTCCAGCAGCGAGTTCAAAGTGTACGGAATGATGAGCTTTTAAGGTTCCGTAAAGCGCAATTACTGTCAGCTTTGAACAGTTTTATTCTCAACAGCATCCCAGTCGCTGTTACATTAACTTCATTCGGTGCATTCACTTTTCTTGGAGGCGAATTGACACCTGCAAGGGCATTTACATCACTTTCTCTTTTTGCTGTGCTACGCTTTCCTTTAAACATGCTGCCCAACTTAATAAGTCAGGTTGTAAATGCAAATGTATCCTTGCAGCGTTTGGAGGAACTGTTTTTAACTGAGGAGCGAATTATAGTACCCAACCCACCACTGGAACCAGGGCTTCCAGCCATCTCAATTCAGGATGGGTACTTTTCATGGGATTCCAAGGCAGGGAAGCCCACATTATCAAACATCAGCTTAGATATACCAGTTGGGAGCTTAGTTGCAGTTGTTGGTGGCACTGGAGAAGGAAAGACATCACTTATATCAGCCATGCTTGGGGAGCTGCCTCCCATTGCGGATTCCAGTGTTGTAATCAGGGGAACTGTTGCTTATGTTCCCCAAGTTTCATGGATTTTCAATGCTACTGTACGTGAAAACATTTTATTTGGATCTGAATTTGACGCTGCTCGGTATTGGAAGGCTATTGATGTAACTGAGTTCCATCATGACCTAAACTTACTTCCAGGTCGAGATCTAACAGAGATAGGTGAAAGAGGGGTCAATATTAGTGGAGGTCAAAAGCAAAGGGTTTCTATGGCTAGAGCTGTATATTCTAATTCAGATGTTTACATATTTGATGACCCATTAAGTGCTCTAGATGCTCATGTTGCTAGAGAGGTTTTCAACCATTGTATCAAGGAAGAGTTGCAGGGGAAAACTCGGGTGCTTGTCACAAATCAGCTGCACTTTCTGCCTCAAGTGGATGAAATTATATTAGTCTCTGATGGTACGATTAAAGAGAAGGGAACCTTTAAGGATCTCTCTGAAAGCAGTTTGCTGTTCCAGAAGCTAATGGAAAATGCCGGGAAAATGGAAGAACATGTAGAAGAAAAGGAAGACAGCAAAATTGATTACCAGGAAAGCTCACTCCCAGTGTCTAACGGGGAGGTGACTGACTTGTCAAACGATGCAAGCTACACCAAGAAAGGGAAAGGGATGAGATCTGTACTCATTAAACAAGAGGAACGGGAAACAGGTGTTGTAAGTTGGAAGAATTTGCAGAGGTACAAACATGCATTAGGAGGCCTATGGGTTGTCATGGTTCTATTTACTTGTTATACACTGATAGAAGTTCTTCGAGTTTCAAGCAGCACATGGTTAAGTTTTTGGACAGACCAAAGCATGTCAGAGAGTTATGCACCTGGATTCTACATTCTTATATATGCGATACTCTCAATTGGTCAGGTAACGGTGACTCTGACGAACTCTTTTTGCCTAATCACTTCAAGTCTTCATGCAGCAAAAAACTTGCATGATCCCCTTCTACATGCTATATTAAAAGCTCCGATGGTATTCTTTCACACTAACCCAACTGGAAGGATAATCAATAGGTTTGCAAAGGATCTGGGTGATATAGATCGCAATGTAGCGAATTTTATGAACATGTTTCTGAGTCAAGTGTGGCAGCTCCTTTCGACTTTTGTGCTCATAGGCATTGTGAGCACAATATCCCTCTGGGCCATAATGCCACTTCTGATTTTGTTCTATGCAGCCTATCTATTCTATCAGAGCACATCCCGTGAAGTGAAACGCATGGATTCCATTACCAGATCTCCCGTTTATGCACTGTTTGGAGAAGCATTAAATGGTTTGTCATCAATTCGTGCCTATGAAGCTTATGATCGGATGGCAAACATCAGTGGTAGGTCTATGGATAATAATATCAGATTCACCCTTGTAAATATTAGTTCAAACCGCTGGCTTGAAATAAGGTTGGAAACACTAGGAGGTGTCATGATTTGGGTGATAGCAACCTTTGCTGTCATGCAAAATGGAAGAGCAGAAAACCAAGTACTATTTGCATCTACAGTTGGTTTACTTCTCACTTATACTTTGAATATCACAAGTCTGTTGAGTGGTGTTCTTAGAAAAGCTAGCAGGGCTGAAAATAGTTTAAATGCCGTTGAGCGGGTTGGAGCATATATAGATTTGCCTTCTGAGGCTCCAGCTGTTATTGAAAGCAACCGTCCCCCACATGGGTGGCCATCATCAGGATCAATCAAGTTTGAGGATGTTGTCCTCCGTTATAGACCTGGACTTCCACCTGTATTGCACGGATTATCATTTACAGTTTCCGCAAGTGAGAGGCTAGGAATAGTTGGAAGAACTGGTGCTGGCAAATCTAGCATGATTAATGCATTGTTTAGGATAGTTGAAATAGAAAAAGGAAGAATTTGTATTGACGGTTGTGATGTTTCCAAGTTTGGACTGACAGATTTACGAAAAGTTCTTAGCATCATACCGCAGGTACCAGTTCTTTTTTCAGGAACTGTGCGGTTTAATCTTGATCCTGTCCGTGAGCATAATGATGCTGACCTTTGGGAAGCCCTAGAAAGAGCACATCTAAGGGATGTCATTTGGAGGAATTCCTTTGGTCTCGATGCTGAGGTTTCTGAGGGCGGTGAGAATTTCAGTGTTGGACAGAGGCAGCTAATAAGTCTTGCTAGAGCATTGCTACGGAGATCAAAGATTCTTGTTCTTGATGAAGCAACTGCAGCTGTGGATGTTAGAACTGATGCTCTCATCCAAAAAACCATACGTGAAGAATTCAAATCATGCACCATGCTCGTGATTGCTCACAGATTGAACACCATTATCGACTGTGATCGAATTCTTGTTCTTGATGCTGGTCAGGTCCTAGAACATGGTTCCCCAGAAGAACTGCTAAATGAAGAGAGCGCATTCTCCAAGATGGTAAGGAGTACAGGGCCTTCTAATGCTCAGTACTTACACAGCCTGGTATTTGAAGGCAAGCAAAATAAGGTGAATAGAGACGAAACCGAGCAACTGGTTGGCCAGAGCCGCAGGAGATGGTTAGCCTCTTCGCACTGGGCCGCTGCTGCCCAATTTGCTTTGACCATTAATCTCGCATCCTCACAGAACGATCTCCAAAGGTTAGATATTGTAGACGAGGACAATATCCTCATGAAAACAAAGGATGCAGTTATAACACTACAGGGAGTTTTGGAAGGGAAGCATGATAAAAATATAGACATATCACTAAATCAGCACCATATTCCAAGAGAGGGGTGGTGGTCAGCTTTCTTCAGAATAGTTGAAGGTCTGGCCGTGATGGGTAGGCTTGCGCAGAACAGACTTCACCCTTTAGAAGATGACGATCGATTTAAAGACCAATCTATAGGTTGGAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006810 GO:0008150 GO:0015399 GO:0015405 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022804 GO:0022857 GO:0031090 GO:0042623 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko01523 ko01524 ko02010 ko04976 map01523 map01524 map02010 map04976

Pfam Domains

Protein Families

ABC_membrane ABC_tran

Protein Analysis

1619

Amino Acids

181.52

Weight (kDa)

6.68

Isoelectric Point (pI)

41.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ABC_membrane	PF00664	300 - 567	4.2e-37	ABC transporter transmembrane region
ABC_tran	PF00005	629 - 764	1.7e-18	ABC transporter
ABC_membrane	PF00664	915 - 1168	6.4e-29	ABC transporter transmembrane region
ABC_tran	PF00005	1252 - 1400	1.2e-25	ABC transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001783)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30410 AT1G30410 AT1G30410 AT1G30410 AT1G30410 AT1G30410 AT1G30420 AT1G30420
fragaria_vesca	FvH4_2g14981 FvH4_2g14981 FvH4_2g14981 FvH4_2g14981 FvH4_2g14981 FvH4_2g14981 FvH4_2g14981 FvH4_3g09710 FvH4_3g09720
malus_domestica	MD10G1255300.v1.1 MD10G1255500.v1.1
prunus_persica	Prupe.4G088600_v2.0.a1 Prupe.4G088600_v2.0.a1
pyrus_communis	pycom10g21050
rosa_chinensis	RchiOBHm_Chr3g0492011 RchiOBHm_Chr5g0015441 RchiOBHm_Chr5g0015451 RchiOBHm_Chr5g0015481 RchiOBHm_Chr5g0015491
rosa_laevigata	RLG00000032199 RLG00000032201 RLG00000032202
rosa_multiflora	Rmu_sc0019960.1_g000002
rosa_roxburghii	Rroxscaffold_1G00061020 Rroxscaffold_1G00061040 Rroxscaffold_1G00061050 Rroxscaffold_6G00392640
rosa_rugosa	Rorug03G0251100 Rorug05G0027400 Rorug05G0027500 Rorug05G0027700
rosa_samantha	Rh3AG301500 Rh5AG121000 Rh5AG121100 Rh5CG130600 Rh5DG118900 Rh5DG119000
rosa_wichuraiana	Rw5G010450 Rw5G010470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	4652
AarI	CACCTGC	1 cut(s)	1657
AasI	GACNNNNNNGTC	2 cut(s)	107, 2602
Acc16I	TGCGCA	1 cut(s)	4791
Acc36I	ACCTGC	2 cut(s)	1657, 3940
Acc65I	GGTACC	1 cut(s)	3952
AccB1I	GGYRCC	1 cut(s)	3952
AccB7I	CCANNNNNTGG	1 cut(s)	855
AccBSI	CCGCTC	1 cut(s)	3613
AccI	GTMKAC	1 cut(s)	4611
AccII	CGCG	1 cut(s)	402
AcoI	YGGCCR	3 cut(s)	3686, 4498, 4770
AcuI	CTGAAG	2 cut(s)	1188, 4735
AdeI	CACNNNGTG	1 cut(s)	92
AflIII	ACRYGT	2 cut(s)	2524, 3087
AleI	CACNNNNGTG	1 cut(s)	3682
Alw21I	GWGCWC	7 cut(s)	359, 871, 2299, 3132, 3149, 3218, 4045
Alw26I	GTCTC	5 cut(s)	791, 1475, 2428, 4085, 4470
AlwNI	CAGNNNCTG	2 cut(s)	1595, 3974
Ama87I	CYCGRG	2 cut(s)	593, 2364
AseI	ATTAAT	2 cut(s)	3837, 4566
Asp700I	GAANNNNTTC	2 cut(s)	1239, 2778
Asp718I	GGTACC	1 cut(s)	3952
AspLEI	GCGC	3 cut(s)	1546, 4385, 4792
AspS9I	GGNCC	8 cut(s)	54, 846, 947, 1159, 3162, 4339, 4412, 4536
AsuC2I	CCSGG	4 cut(s)	187, 594, 595, 2509
AsuNHI	GCTAGC	1 cut(s)	3578
AvaI	CYCGRG	2 cut(s)	593, 2364
AvaII	GGWCC	3 cut(s)	846, 947, 4339
BaeI	ACNNNNGTAYC	2 cut(s)	2320, 2353
BalI	TGGCCA	2 cut(s)	3688, 4500
BamHI	GGATCC	2 cut(s)	583, 1325
BanI	GGYRCC	1 cut(s)	3952
BarI	GAAGNNNNNNTAC	4 cut(s)	1269, 1301, 4399, 4431
BauI	CACGAG	1 cut(s)	4271
BbsI	GAAGAC	2 cut(s)	2547, 2927
Bbv12I	GWGCWC	7 cut(s)	359, 871, 2299, 3132, 3149, 3218, 4045
BbvCI	CCTCAGC	1 cut(s)	4089
BceAI	ACGGC	4 cut(s)	692, 1146, 3590, 4757
BcgI	CGANNNNNNTGC	6 cut(s)	1573, 1607, 3109, 3143, 4252, 4286
BciVI	GTATCC	2 cut(s)	1450, 1756
BclI	TGATCA	2 cut(s)	165, 1368
BcnI	CCSGG	4 cut(s)	187, 594, 595, 2509
BcoDI	GTCTC	5 cut(s)	791, 1475, 2428, 4085, 4470
BfmI	CTRYAG	5 cut(s)	493, 3510, 4197, 4658, 4842
BfuAI	ACCTGC	2 cut(s)	1657, 3940
BfuI	GTATCC	2 cut(s)	1450, 1756
BglII	AGATCT	3 cut(s)	2176, 2645, 3254
BlpI	GCTNAGC	1 cut(s)	255
BmcAI	AGTACT	2 cut(s)	3498, 4430
Bme18I	GGWCC	3 cut(s)	846, 947, 4339
BmeT110I	CYCGRG	2 cut(s)	593, 2364
BmgT120I	GGNCC	8 cut(s)	54, 846, 947, 1159, 3162, 4339, 4412, 4536
BmrI	ACTGGG	4 cut(s)	1188, 1583, 2570, 4543
BmtI	GCTAGC	1 cut(s)	3582
BmuI	ACTGGG	4 cut(s)	1188, 1583, 2570, 4543
BoxI	GACNNNNGTC	1 cut(s)	1252
BpiI	GAAGAC	2 cut(s)	2547, 2927
BplI	GAGNNNNNCTC	2 cut(s)	1413, 1445
BpmI	CTGGAG	5 cut(s)	672, 679, 1205, 1965, 3633
Bpu10I	CCTNAGC	1 cut(s)	4089
Bpu1102I	GCTNAGC	1 cut(s)	255
BpuEI	CTTGAG	1 cut(s)	2385
BpuMI	CCSGG	4 cut(s)	187, 594, 595, 2509
Bsa29I	ATCGAT	1 cut(s)	4825
BsaAI	YACGTR	3 cut(s)	418, 2075, 4244
BsaBI	GATNNNNATC	3 cut(s)	588, 3060, 4821
BsaI	GGTCTC	1 cut(s)	4085
BsaJI	CCNNGG	7 cut(s)	593, 854, 883, 1452, 1476, 1817, 1866
BsaXI	ACNNNNNCTCC	6 cut(s)	441, 471, 1754, 1784, 3241, 3271
Bse3DI	GCAATG	5 cut(s)	102, 931, 1995, 3073, 4154
Bse8I	GATNNNNATC	3 cut(s)	588, 3060, 4821
BseCI	ATCGAT	1 cut(s)	4825
BseDI	CCNNGG	7 cut(s)	593, 854, 883, 1452, 1476, 1817, 1866
BseJI	GATNNNNATC	3 cut(s)	588, 3060, 4821
BseMI	GCAATG	5 cut(s)	102, 931, 1995, 3073, 4154
BseRI	GAGGAG	1 cut(s)	1796
BseYI	CCCAGC	1 cut(s)	825
BsgI	GTGCAG	1 cut(s)	2372
Bsh1236I	CGCG	1 cut(s)	402
Bsh1285I	CGRYCG	1 cut(s)	4825
BshNI	GGYRCC	1 cut(s)	3952
BshVI	ATCGAT	1 cut(s)	4825
BsiEI	CGRYCG	1 cut(s)	4825
BsiHKAI	GWGCWC	7 cut(s)	359, 871, 2299, 3132, 3149, 3218, 4045
BsiHKCI	CYCGRG	2 cut(s)	593, 2364
BsiSI	CCGG	3 cut(s)	186, 594, 2508
BslFI	GGGAC	2 cut(s)	933, 3657
BsmAI	GTCTC	5 cut(s)	791, 1475, 2428, 4085, 4470
BsmBI	CGTCTC	1 cut(s)	4470
BsmFI	GGGAC	2 cut(s)	933, 3657
BsmI	GAATGC	4 cut(s)	410, 827, 1619, 4385
Bso31I	GGTCTC	1 cut(s)	4085
BsoBI	CYCGRG	2 cut(s)	593, 2364
Bsp1286I	GDGCHC	7 cut(s)	359, 871, 2299, 3132, 3149, 3218, 4045
Bsp1720I	GCTNAGC	1 cut(s)	255
Bsp19I	CCATGG	2 cut(s)	854, 1452
Bsp68I	TCGCGA	1 cut(s)	402
BspDI	ATCGAT	1 cut(s)	4825
BspFNI	CGCG	1 cut(s)	402
BspHI	TCATGA	5 cut(s)	1344, 1431, 2149, 3438, 4629
BspMAI	CTGCAG	1 cut(s)	4201
BspMI	ACCTGC	2 cut(s)	1657, 3940
BspOI	GCTAGC	1 cut(s)	3582
BspQI	GCTCTTC	1 cut(s)	3475
BspT107I	GGYRCC	1 cut(s)	3952
BspTNI	GGTCTC	1 cut(s)	4085
BsrBI	CCGCTC	1 cut(s)	3613
BsrDI	GCAATG	5 cut(s)	102, 931, 1995, 3073, 4154
BssECI	CCNNGG	7 cut(s)	593, 854, 883, 1452, 1476, 1817, 1866
BssSI	CACGAG	1 cut(s)	4271
BssT1I	CCWWGG	4 cut(s)	854, 883, 1452, 1866
Bst2BI	CACGAG	1 cut(s)	4271
Bst6I	CTCTTC	4 cut(s)	2340, 3475, 4371, 4531
BstAPI	GCANNNNNTGC	4 cut(s)	68, 979, 2395, 3461
BstBAI	YACGTR	3 cut(s)	418, 2075, 4244
BstC8I	GCNNGC	7 cut(s)	1231, 2617, 3404, 3580, 3820, 4457, 4788
BstDSI	CCRYGG	2 cut(s)	854, 1452
BstFNI	CGCG	1 cut(s)	402
BstHHI	GCGC	3 cut(s)	1546, 4385, 4792
BstMAI	GTCTC	5 cut(s)	791, 1475, 2428, 4085, 4470
BstMCI	CGRYCG	1 cut(s)	4825
BstNSI	RCATGY	8 cut(s)	683, 1233, 1708, 2528, 2720, 2830, 2975, 3091
BstPAI	GACNNNNGTC	1 cut(s)	1252
BstSFI	CTRYAG	5 cut(s)	493, 3510, 4197, 4658, 4842
BstSNI	TACGTA	1 cut(s)	418
BstUI	CGCG	1 cut(s)	402
BstV2I	GAAGAC	2 cut(s)	2547, 2927
BstX2I	RGATCY	8 cut(s)	583, 1325, 2093, 2176, 2461, 2645, 3046, 3254
BstXI	CCANNNNNNTGG	2 cut(s)	21, 3684
BstYI	RGATCY	8 cut(s)	583, 1325, 2093, 2176, 2461, 2645, 3046, 3254
Bsu15I	ATCGAT	1 cut(s)	4825
BsuI	GTATCC	2 cut(s)	1450, 1756
BsuTUI	ATCGAT	1 cut(s)	4825
BtgI	CCRYGG	2 cut(s)	854, 1452
BtgZI	GCGATG	1 cut(s)	1299
BtsI	GCAGTG	1 cut(s)	249
BtuMI	TCGCGA	1 cut(s)	402
BveI	ACCTGC	2 cut(s)	1657, 3940
Cac8I	GCNNGC	7 cut(s)	1231, 2617, 3404, 3580, 3820, 4457, 4788
CaiI	CAGNNNCTG	2 cut(s)	1595, 3974
CciI	TCATGA	5 cut(s)	1344, 1431, 2149, 3438, 4629
CfoI	GCGC	3 cut(s)	1546, 4385, 4792
Cfr13I	GGNCC	8 cut(s)	54, 846, 947, 1159, 3162, 4339, 4412, 4536
Cfr9I	CCCGGG	1 cut(s)	593
ClaI	ATCGAT	1 cut(s)	4825
CseI	GACGC	1 cut(s)	2114
CspCI	CAANNNNNGTGG	4 cut(s)	2194, 2229, 3735, 3770
DraI	TTTAAA	3 cut(s)	1701, 3600, 4831
DraIII	CACNNNGTG	1 cut(s)	92
DrdI	GACNNNNNNGTC	2 cut(s)	107, 2602
DseDI	GACNNNNNNGTC	2 cut(s)	107, 2602
EaeI	YGGCCR	3 cut(s)	3686, 4498, 4770
Eam1104I	CTCTTC	4 cut(s)	2340, 3475, 4371, 4531
EarI	CTCTTC	4 cut(s)	2340, 3475, 4371, 4531
Eco105I	TACGTA	1 cut(s)	418
Eco130I	CCWWGG	4 cut(s)	854, 883, 1452, 1866
Eco147I	AGGCCT	1 cut(s)	2730
Eco31I	GGTCTC	1 cut(s)	4085
Eco32I	GATATC	1 cut(s)	1200
Eco47I	GGWCC	3 cut(s)	846, 947, 4339
Eco57I	CTGAAG	2 cut(s)	1188, 4735
Eco88I	CYCGRG	2 cut(s)	593, 2364
EcoO109I	RGGNCCY	3 cut(s)	947, 4339, 4412
EcoRI	GAATTC	4 cut(s)	552, 4069, 4250, 4314
EcoRV	GATATC	1 cut(s)	1200
EcoT14I	CCWWGG	4 cut(s)	854, 883, 1452, 1866
EcoT22I	ATGCAT	2 cut(s)	2722, 3844
ErhI	CCWWGG	4 cut(s)	854, 883, 1452, 1866
Esp3I	CGTCTC	1 cut(s)	4470
FalI	AAGNNNNNCTT	2 cut(s)	2438, 2470
FaqI	GGGAC	2 cut(s)	933, 3657
FauI	CCCGC	2 cut(s)	1080, 3606
FbaI	TGATCA	2 cut(s)	165, 1368
FblI	GTMKAC	1 cut(s)	4611
FspI	TGCGCA	1 cut(s)	4791
GlaI	GCGC	3 cut(s)	1545, 4384, 4791
GsaI	CCCAGC	1 cut(s)	829
GsuI	CTGGAG	5 cut(s)	672, 679, 1205, 1965, 3633
HapII	CCGG	3 cut(s)	186, 594, 2508
HgaI	GACGC	1 cut(s)	2114
HhaI	GCGC	3 cut(s)	1546, 4385, 4792
Hin6I	GCGC	3 cut(s)	1544, 4383, 4790
HinP1I	GCGC	3 cut(s)	1544, 4383, 4790
HindIII	AAGCTT	2 cut(s)	917, 3318
HpaII	CCGG	3 cut(s)	186, 594, 2508
Hpy166II	GTNNAC	4 cut(s)	1511, 2272, 3521, 4612
Hpy8I	GTNNAC	4 cut(s)	1511, 2272, 3521, 4612
HpyCH4IV	ACGT	3 cut(s)	417, 2074, 4243
HpySE526I	ACGT	3 cut(s)	417, 2074, 4243
HspAI	GCGC	3 cut(s)	1544, 4383, 4790
KflI	GGGWCCC	1 cut(s)	947
KpnI	GGTACC	1 cut(s)	3956
Ksp22I	TGATCA	2 cut(s)	165, 1368
LguI	GCTCTTC	1 cut(s)	3475
LmnI	GCTCC	9 cut(s)	316, 1264, 1783, 1917, 1984, 2992, 3117, 3620, 3652
MaeII	ACGT	3 cut(s)	417, 2074, 4243
MaeIII	GTNAC	9 cut(s)	242, 481, 618, 1597, 2134, 2374, 2593, 2890, 2896
MbiI	CCGCTC	1 cut(s)	3613
MfeI	CAATTG	1 cut(s)	2880
MflI	RGATCY	8 cut(s)	583, 1325, 2093, 2176, 2461, 2645, 3046, 3254
MhlI	GDGCHC	7 cut(s)	359, 871, 2299, 3132, 3149, 3218, 4045
MlsI	TGGCCA	2 cut(s)	3688, 4500
MluNI	TGGCCA	2 cut(s)	3688, 4500
MlyI	GAGTC	6 cut(s)	95, 438, 626, 712, 2893, 3106
MmeI	TCCRAC	8 cut(s)	756, 1199, 2672, 3399, 3598, 3784, 4102, 4829
Mox20I	TGGCCA	2 cut(s)	3688, 4500
Mph1103I	ATGCAT	2 cut(s)	2722, 3844
MroXI	GAANNNNTTC	2 cut(s)	1239, 2778
MscI	TGGCCA	2 cut(s)	3688, 4500
MslI	CAYNNNNRTG	4 cut(s)	1037, 3682, 4011, 4271
Msp20I	TGGCCA	2 cut(s)	3688, 4500
MspA1I	CMGCKG	5 cut(s)	2386, 3402, 3653, 4202, 4541
MspI	CCGG	3 cut(s)	186, 594, 2508
MunI	CAATTG	1 cut(s)	2880
Mva1269I	GAATGC	4 cut(s)	410, 827, 1619, 4385
MvnI	CGCG	1 cut(s)	402
NciI	CCSGG	4 cut(s)	187, 594, 595, 2509
NcoI	CCATGG	2 cut(s)	854, 1452
NheI	GCTAGC	1 cut(s)	3578
NmuCI	GTSAC	5 cut(s)	481, 618, 2374, 2593, 2896
NruI	TCGCGA	1 cut(s)	402
NsbI	TGCGCA	1 cut(s)	4791
NsiI	ATGCAT	2 cut(s)	2722, 3844
NspI	RCATGY	8 cut(s)	683, 1233, 1708, 2528, 2720, 2830, 2975, 3091
OliI	CACNNNNGTG	1 cut(s)	3682
PaeI	GCATGC	1 cut(s)	1233
PagI	TCATGA	5 cut(s)	1344, 1431, 2149, 3438, 4629
PaqCI	CACCTGC	1 cut(s)	1657
PceI	AGGCCT	1 cut(s)	2730
PciI	ACATGT	2 cut(s)	2524, 3087
PciSI	GCTCTTC	1 cut(s)	3475
PctI	GAATGC	4 cut(s)	410, 827, 1619, 4385
PdmI	GAANNNNTTC	2 cut(s)	1239, 2778
PfeI	GAWTC	9 cut(s)	836, 894, 1061, 1862, 2003, 2850, 3242, 3372, 4174
PflMI	CCANNNNNTGG	1 cut(s)	855
Ple19I	CGATCG	1 cut(s)	4825
PleI	GAGTC	6 cut(s)	95, 437, 625, 712, 2893, 3105
PpsI	GAGTC	6 cut(s)	95, 437, 625, 712, 2893, 3105
Ppu21I	YACGTR	3 cut(s)	418, 2075, 4244
PpuMI	RGGWCCY	2 cut(s)	947, 4339
PscI	ACATGT	2 cut(s)	2524, 3087
PshAI	GACNNNNGTC	1 cut(s)	1252
PshBI	ATTAAT	2 cut(s)	3837, 4566
PsiI	TTATAA	1 cut(s)	4652
Psp5II	RGGWCCY	2 cut(s)	947, 4339
PspFI	CCCAGC	1 cut(s)	825
PspPI	GGNCC	8 cut(s)	54, 846, 947, 1159, 3162, 4339, 4412, 4536
PspPPI	RGGWCCY	2 cut(s)	947, 4339
PstI	CTGCAG	1 cut(s)	4201
PstNI	CAGNNNCTG	2 cut(s)	1595, 3974
PsuI	RGATCY	8 cut(s)	583, 1325, 2093, 2176, 2461, 2645, 3046, 3254
PvuI	CGATCG	1 cut(s)	4825
PvuII	CAGCTG	3 cut(s)	2386, 3653, 4202
RruI	TCGCGA	1 cut(s)	402
RseI	CAYNNNNRTG	4 cut(s)	1037, 3682, 4011, 4271
SapI	GCTCTTC	1 cut(s)	3475
Sau96I	GGNCC	8 cut(s)	54, 846, 947, 1159, 3162, 4339, 4412, 4536
ScaI	AGTACT	2 cut(s)	3498, 4430
SchI	GAGTC	6 cut(s)	95, 438, 626, 712, 2893, 3106
SduI	GDGCHC	7 cut(s)	359, 871, 2299, 3132, 3149, 3218, 4045
SfcI	CTRYAG	5 cut(s)	493, 3510, 4197, 4658, 4842
SinI	GGWCC	3 cut(s)	846, 947, 4339
SmaI	CCCGGG	1 cut(s)	595
SmiMI	CAYNNNNRTG	4 cut(s)	1037, 3682, 4011, 4271
SmlI	CTYRAG	1 cut(s)	2400
SmoI	CTYRAG	1 cut(s)	2400
SnaBI	TACGTA	1 cut(s)	418
SphI	GCATGC	1 cut(s)	1233
SseBI	AGGCCT	1 cut(s)	2730
SspI	AATATT	2 cut(s)	2209, 3388
StuI	AGGCCT	1 cut(s)	2730
StyI	CCWWGG	4 cut(s)	854, 883, 1452, 1866
TaiI	ACGT	3 cut(s)	420, 2077, 4246
TaqII	GACCGA	1 cut(s)	809
TatI	WGTACW	4 cut(s)	2650, 3496, 4406, 4428
TauI	GCSGC	2 cut(s)	4509, 4541
TfiI	GAWTC	9 cut(s)	836, 894, 1061, 1862, 2003, 2850, 3242, 3372, 4174
TseFI	GTSAC	5 cut(s)	481, 618, 2374, 2593, 2896
Tsp45I	GTSAC	5 cut(s)	481, 618, 2374, 2593, 2896
TspGWI	ACGGA	8 cut(s)	1121, 1260, 1526, 1528, 3716, 3774, 3989, 4177
TspMI	CCCGGG	1 cut(s)	593
Van91I	CCANNNNNTGG	1 cut(s)	855
VpaK11BI	GGWCC	3 cut(s)	846, 947, 4339
VspI	ATTAAT	2 cut(s)	3837, 4566
XbaI	TCTAGA	1 cut(s)	2299
XceI	RCATGY	8 cut(s)	683, 1233, 1708, 2528, 2720, 2830, 2975, 3091
XcmI	CCANNNNNNNNNTGG	3 cut(s)	545, 1807, 4731
XmaI	CCCGGG	1 cut(s)	593
XmiI	GTMKAC	1 cut(s)	4611
XmnI	GAANNNNTTC	2 cut(s)	1239, 2778
ZrmI	AGTACT	2 cut(s)	3498, 4430
Zsp2I	ATGCAT	2 cut(s)	2722, 3844

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.