MD11G1061200.v1.1

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0003466
Physical Location & Seq
Forward (+)
5324074 .. 5325955
1882 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1061200.v1.1.491

Sequence Viewer

Length: 969 bp
ATGGCTATTCTCCGATCATTTCTAGCGATCATCTGTATTGTATTGGTTGCTGCCACTAGTATGAAACCAACATCTGCAAATCTAACTACTCATTTCTACACGAAAGGGTGCCCTCAGGCACTACCAGCCATCAGAAAAGCTGTCGAGCGGGCCATCAAACGCGAGCCACGGATGGGGGCCTCTCTAGTTCGCCTGCACTTCCATGACTGCTTTGTTAATGGGTGTGATGAGTCAATTCTGCTTGATGACACTTCCAACTTCACCGGTGAGAAGACAGCCGTGCCAAATGCGAATTCGATCAGGGGATTAAACGTGGTCGATGATATCAAAAAAGCTGTTGACAAAGCTTGCAAGAAAAGTGTGGTCTCATGCGCAGATATATTAGCAGTTGCAGCTCGTGATTCCGTTTCCATTTTGGGAGGCCCAACTTACAAAGTACAATTGGGAAGAAGGGATGCAAGAACTGCAAGCGTAAATGATGCAAACAGAAATCTTCCTCCTCCATTCTTCAACTTCCCACAGCTTCTCTCAAACTTCGAAGCTCATGGTCTAAACCTAAAAGACTTAGTTGTGCTCTCAGCAGCCCACACCCTTGGACTTGCTCGGTGCACCACCTTCCGTGCCAGGATTTATAACGATACCAATATAGACCCACAATTTGCAGCCTTGGCTAAAAAAAATTGCCCAACAAGTGGAGGAGATGACAACACAAGGCCACTAGATGCAACTTCAAAGAGATTCGATACTGTCTACTTTGAGGCTCTGTTGAAATCAAAGGGTCTCCTCCACTCTGATCAGGAGCTCTTTAAGGGTAATGGTAGCGATAGTGATAAGTTGGTGCAGCATTACAGCAAGAACTCAGCTGCTTTTGGAAAAGACTTCGCAAATTCTATGATCAAGATGGGCAACATCAAGCCTCTCACTGGAAACGAGGGAGAAGTCAGACTGGACTGCAGGAAAATCAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
peroxidase

Protein Analysis

323

Amino Acids

35.06

Weight (kDa)

9.24

Isoelectric Point (pI)

25.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 44 - 286 5e-80 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 633
Acc16I TGCGCA 1 cut(s) 373
AccB1I GGYRCC 1 cut(s) 108
AccB7I CCANNNNNTGG 1 cut(s) 692
AccBSI CCGCTC 1 cut(s) 148
AccI GTMKAC 1 cut(s) 750
AccII CGCG 1 cut(s) 162
AciI CCGC 1 cut(s) 148
AcsI RAATTY 2 cut(s) 292, 886
AfaI GTAC 1 cut(s) 438
AfiI CCNNNNNNNGG 3 cut(s) 173, 692, 923
AgeI ACCGGT 1 cut(s) 263
AgsI TTSAA 3 cut(s) 511, 732, 769
AhlI ACTAGT 1 cut(s) 56
AjnI CCWGG 1 cut(s) 623
AjuI GAANNNNNNNTTGG 2 cut(s) 277, 309
AluBI AGCT 8 cut(s) 140, 335, 347, 395, 523, 542, 802, 863
AluI AGCT 8 cut(s) 140, 335, 347, 395, 523, 542, 802, 863
Alw21I GWGCWC 3 cut(s) 576, 611, 804
Alw26I GTCTC 2 cut(s) 370, 785
Alw44I GTGCAC 1 cut(s) 607
AoxI GGCC 4 cut(s) 150, 177, 421, 713
ApaLI GTGCAC 1 cut(s) 607
ApeKI GCWGC 6 cut(s) 50, 392, 581, 662, 841, 863
ApoI RAATTY 2 cut(s) 292, 886
ArsI GACNNNNNNTTYG 2 cut(s) 641, 673
AsiGI ACCGGT 1 cut(s) 263
AspLEI GCGC 1 cut(s) 374
AspS9I GGNCC 3 cut(s) 150, 177, 422
AsuHPI GGTGA 2 cut(s) 253, 278
AsuII TTCGAA 1 cut(s) 537
AxyI CCTNAGG 1 cut(s) 114
BaeGI GKGCMC 2 cut(s) 113, 611
BanI GGYRCC 1 cut(s) 108
BanII GRGCYC 1 cut(s) 804
BauI CACGAG 1 cut(s) 396
BbsI GAAGAC 1 cut(s) 278
Bbv12I GWGCWC 3 cut(s) 576, 611, 804
BbvI GCAGC 6 cut(s) 37, 404, 593, 674, 850, 853
BccI CCATC 4 cut(s) 137, 161, 166, 895
BceAI ACGGC 1 cut(s) 263
BciT130I CCWGG 1 cut(s) 625
BclI TGATCA 2 cut(s) 793, 894
BcoDI GTCTC 2 cut(s) 370, 785
BcuI ACTAGT 1 cut(s) 56
BfaI CTAG 5 cut(s) 23, 57, 185, 719, 967
BfmI CTRYAG 1 cut(s) 952
BisI GCNGC 6 cut(s) 51, 393, 582, 663, 842, 864
BlsI GCNGC 6 cut(s) 52, 394, 583, 664, 843, 865
Bme1390I CCNGG 1 cut(s) 625
BmgT120I GGNCC 3 cut(s) 150, 177, 422
BmiI GGNNCC 2 cut(s) 110, 178
BmrFI CCNGG 1 cut(s) 625
BmsI GCATC 3 cut(s) 445, 469, 712
BpiI GAAGAC 1 cut(s) 278
Bpu14I TTCGAA 1 cut(s) 537
BsaI GGTCTC 2 cut(s) 370, 785
BsaJI CCNNGG 3 cut(s) 167, 592, 666
BsaWI WCCGGW 1 cut(s) 263
Bsc4I CCNNNNNNNGG 3 cut(s) 173, 692, 923
Bse118I RCCGGY 1 cut(s) 263
Bse1I ACTGG 2 cut(s) 928, 951
Bse21I CCTNAGG 1 cut(s) 114
BseBI CCWGG 1 cut(s) 625
BseDI CCNNGG 3 cut(s) 167, 592, 666
BseGI GGATG 2 cut(s) 177, 460
BseLI CCNNNNNNNGG 3 cut(s) 173, 692, 923
BseMII CTCAG 3 cut(s) 128, 591, 873
BseNI ACTGG 2 cut(s) 928, 951
BseRI GAGGAG 3 cut(s) 489, 711, 773
BseSI GKGCMC 2 cut(s) 113, 611
BseXI GCAGC 6 cut(s) 37, 404, 593, 674, 850, 853
BsgI GTGCAG 2 cut(s) 179, 860
Bsh1236I CGCG 1 cut(s) 162
BshFI GGCC 4 cut(s) 152, 179, 423, 715
BshNI GGYRCC 1 cut(s) 108
BshTI ACCGGT 1 cut(s) 263
BsiHKAI GWGCWC 3 cut(s) 576, 611, 804
BsiSI CCGG 1 cut(s) 264
BslI CCNNNNNNNGG 3 cut(s) 173, 692, 923
BsmAI GTCTC 2 cut(s) 370, 785
BsnI GGCC 4 cut(s) 152, 179, 423, 715
Bso31I GGTCTC 2 cut(s) 370, 785
Bsp119I TTCGAA 1 cut(s) 537
Bsp1286I GDGCHC 4 cut(s) 113, 576, 611, 804
Bsp143I GATC 5 cut(s) 14, 27, 297, 793, 894
BspACI CCGC 1 cut(s) 148
BspANI GGCC 4 cut(s) 152, 179, 423, 715
BspCNI CTCAG 3 cut(s) 127, 590, 872
BspFNI CGCG 1 cut(s) 162
BspLI GGNNCC 2 cut(s) 110, 178
BspMAI CTGCAG 1 cut(s) 956
BspT104I TTCGAA 1 cut(s) 537
BspT107I GGYRCC 1 cut(s) 108
BspTNI GGTCTC 2 cut(s) 370, 785
BsrBI CCGCTC 1 cut(s) 148
BsrFI RCCGGY 1 cut(s) 263
BsrI ACTGG 2 cut(s) 928, 951
BssAI RCCGGY 1 cut(s) 263
BssECI CCNNGG 3 cut(s) 167, 592, 666
BssMI GATC 5 cut(s) 14, 27, 297, 793, 894
BssSI CACGAG 1 cut(s) 396
BssT1I CCWWGG 2 cut(s) 592, 666
Bst2BI CACGAG 1 cut(s) 396
Bst2UI CCWGG 1 cut(s) 625
Bst4CI ACNGT 1 cut(s) 748
BstAPI GCANNNNNTGC 1 cut(s) 464
BstBI TTCGAA 1 cut(s) 537
BstC8I GCNNGC 5 cut(s) 150, 164, 194, 349, 469
BstDEI CTNAG 4 cut(s) 114, 565, 577, 859
BstDSI CCRYGG 1 cut(s) 167
BstF5I GGATG 2 cut(s) 177, 460
BstFNI CGCG 1 cut(s) 162
BstHHI GCGC 1 cut(s) 374
BstKTI GATC 5 cut(s) 17, 30, 300, 796, 897
BstMAI GTCTC 2 cut(s) 370, 785
BstMBI GATC 5 cut(s) 14, 27, 297, 793, 894
BstMWI GCNNNNNNNGC 4 cut(s) 125, 392, 464, 668
BstNI CCWGG 1 cut(s) 625
BstSCI CCNGG 1 cut(s) 623
BstSFI CTRYAG 1 cut(s) 952
BstSLI GKGCMC 2 cut(s) 113, 611
BstUI CGCG 1 cut(s) 162
BstV1I GCAGC 6 cut(s) 37, 404, 593, 674, 850, 853
BstV2I GAAGAC 1 cut(s) 278
BstXI CCANNNNNNTGG 1 cut(s) 593
Bsu36I CCTNAGG 1 cut(s) 114
BsuRI GGCC 4 cut(s) 152, 179, 423, 715
BtgI CCRYGG 1 cut(s) 167
BtsCI GGATG 2 cut(s) 177, 460
BtsIMutI CAGTG 1 cut(s) 921
Cac8I GCNNGC 5 cut(s) 150, 164, 194, 349, 469
CfoI GCGC 1 cut(s) 374
Cfr10I RCCGGY 1 cut(s) 263
Cfr13I GGNCC 3 cut(s) 150, 177, 422
Csp6I GTAC 1 cut(s) 437
CspAI ACCGGT 1 cut(s) 263
CviAII CATG 3 cut(s) 203, 369, 545
CviQI GTAC 1 cut(s) 437
DdeI CTNAG 4 cut(s) 114, 565, 577, 859
DpnI GATC 5 cut(s) 16, 29, 299, 795, 896
DpnII GATC 5 cut(s) 14, 27, 297, 793, 894
Ecl136II GAGCTC 1 cut(s) 802
Eco130I CCWWGG 2 cut(s) 592, 666
Eco24I GRGCYC 1 cut(s) 804
Eco31I GGTCTC 2 cut(s) 370, 785
Eco32I GATATC 1 cut(s) 325
Eco53kI GAGCTC 1 cut(s) 802
Eco81I CCTNAGG 1 cut(s) 114
EcoICRI GAGCTC 1 cut(s) 802
EcoO109I RGGNCCY 1 cut(s) 177
EcoRI GAATTC 1 cut(s) 292
EcoRII CCWGG 1 cut(s) 623
EcoRV GATATC 1 cut(s) 325
EcoT14I CCWWGG 2 cut(s) 592, 666
EcoT38I GRGCYC 1 cut(s) 804
ErhI CCWWGG 2 cut(s) 592, 666
FaeI CATG 3 cut(s) 206, 372, 548
FaiI YATR 8 cut(s) 62, 204, 370, 380, 546, 633, 647, 893
FatI CATG 3 cut(s) 202, 368, 544
FauI CCCGC 1 cut(s) 141
FbaI TGATCA 2 cut(s) 793, 894
FblI GTMKAC 1 cut(s) 750
Fnu4HI GCNGC 6 cut(s) 51, 393, 582, 663, 842, 864
FokI GGATG 2 cut(s) 184, 467
FriOI GRGCYC 1 cut(s) 804
Fsp4HI GCNGC 6 cut(s) 51, 393, 582, 663, 842, 864
FspBI CTAG 5 cut(s) 23, 57, 185, 719, 967
FspI TGCGCA 1 cut(s) 373
GlaI GCGC 1 cut(s) 373
GluI GCNGC 6 cut(s) 51, 393, 582, 663, 842, 864
HaeIII GGCC 4 cut(s) 152, 179, 423, 715
HapII CCGG 1 cut(s) 264
HhaI GCGC 1 cut(s) 374
Hin1II CATG 3 cut(s) 206, 372, 548
Hin6I GCGC 1 cut(s) 372
HinP1I GCGC 1 cut(s) 372
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HindIII AAGCTT 1 cut(s) 345
HinfI GANTC 3 cut(s) 230, 401, 738
HpaII CCGG 1 cut(s) 264
HphI GGTGA 2 cut(s) 253, 278
Hpy166II GTNNAC 3 cut(s) 340, 609, 751
Hpy188I TCNGA 4 cut(s) 14, 134, 793, 944
Hpy188III TCNNGA 3 cut(s) 398, 797, 898
Hpy8I GTNNAC 3 cut(s) 340, 609, 751
HpyAV CCTTC 2 cut(s) 444, 625
HpyCH4III ACNGT 1 cut(s) 748
HpyCH4IV ACGT 1 cut(s) 312
HpyF10VI GCNNNNNNNGC 4 cut(s) 125, 392, 464, 668
HpyF3I CTNAG 4 cut(s) 114, 565, 577, 859
HpySE526I ACGT 1 cut(s) 312
Hsp92II CATG 3 cut(s) 206, 372, 548
HspAI GCGC 1 cut(s) 372
Ksp22I TGATCA 2 cut(s) 793, 894
Kzo9I GATC 5 cut(s) 14, 27, 297, 793, 894
LmnI GCTCC 1 cut(s) 799
Lsp1109I GCAGC 6 cut(s) 37, 404, 593, 674, 850, 853
LweI GCATC 3 cut(s) 445, 469, 712
MaeI CTAG 5 cut(s) 23, 57, 185, 719, 967
MaeII ACGT 1 cut(s) 312
MalI GATC 5 cut(s) 16, 29, 299, 795, 896
MbiI CCGCTC 1 cut(s) 148
MboI GATC 5 cut(s) 14, 27, 297, 793, 894
MboII GAAGA 4 cut(s) 283, 459, 485, 499
MfeI CAATTG 1 cut(s) 440
MhlI GDGCHC 4 cut(s) 113, 576, 611, 804
MluCI AATT 6 cut(s) 234, 292, 440, 656, 679, 886
MlyI GAGTC 1 cut(s) 239
MmeI TCCRAC 1 cut(s) 279
MseI TTAA 3 cut(s) 216, 308, 807
MslI CAYNNNNRTG 2 cut(s) 59, 201
MspA1I CMGCKG 1 cut(s) 863
MspI CCGG 1 cut(s) 264
MspR9I CCNGG 1 cut(s) 625
MunI CAATTG 1 cut(s) 440
MvaI CCWGG 1 cut(s) 625
MvnI CGCG 1 cut(s) 162
MwoI GCNNNNNNNGC 4 cut(s) 125, 392, 464, 668
NdeII GATC 5 cut(s) 14, 27, 297, 793, 894
NlaIII CATG 3 cut(s) 206, 372, 548
NlaIV GGNNCC 2 cut(s) 110, 178
NsbI TGCGCA 1 cut(s) 373
NspV TTCGAA 1 cut(s) 537
PfeI GAWTC 2 cut(s) 401, 738
PflMI CCANNNNNTGG 1 cut(s) 692
PinAI ACCGGT 1 cut(s) 263
PkrI GCNGC 6 cut(s) 52, 394, 583, 664, 843, 865
PleI GAGTC 1 cut(s) 238
PpsI GAGTC 1 cut(s) 238
PsiI TTATAA 1 cut(s) 633
Psp124BI GAGCTC 1 cut(s) 804
Psp6I CCWGG 1 cut(s) 623
PspGI CCWGG 1 cut(s) 623
PspN4I GGNNCC 2 cut(s) 110, 178
PspPI GGNCC 3 cut(s) 150, 177, 422
PstI CTGCAG 1 cut(s) 956
PvuII CAGCTG 1 cut(s) 863
RsaI GTAC 1 cut(s) 438
RsaNI GTAC 1 cut(s) 437
RseI CAYNNNNRTG 2 cut(s) 59, 201
SacI GAGCTC 1 cut(s) 804
SaqAI TTAA 3 cut(s) 216, 308, 807
SatI GCNGC 6 cut(s) 51, 393, 582, 663, 842, 864
Sau3AI GATC 5 cut(s) 14, 27, 297, 793, 894
Sau96I GGNCC 3 cut(s) 150, 177, 422
SchI GAGTC 1 cut(s) 239
ScrFI CCNGG 1 cut(s) 625
SduI GDGCHC 4 cut(s) 113, 576, 611, 804
SfaNI GCATC 3 cut(s) 445, 469, 712
SfcI CTRYAG 1 cut(s) 952
SfuI TTCGAA 1 cut(s) 537
SgrAI CRCCGGYG 1 cut(s) 263
SmiMI CAYNNNNRTG 2 cut(s) 59, 201
SpeI ACTAGT 1 cut(s) 56
Sse9I AATT 6 cut(s) 234, 292, 440, 656, 679, 886
SsiI CCGC 1 cut(s) 148
SspMI CTAG 5 cut(s) 23, 57, 185, 719, 967
SstI GAGCTC 1 cut(s) 804
StyD4I CCNGG 1 cut(s) 623
StyI CCWWGG 2 cut(s) 592, 666
TaaI ACNGT 1 cut(s) 748
TaiI ACGT 1 cut(s) 315
TaqI TCGA 5 cut(s) 144, 296, 318, 537, 741
TasI AATT 6 cut(s) 234, 292, 440, 656, 679, 886
TatI WGTACW 1 cut(s) 436
TfiI GAWTC 2 cut(s) 401, 738
Tru1I TTAA 3 cut(s) 216, 308, 807
Tru9I TTAA 3 cut(s) 216, 308, 807
TscAI CASTG 1 cut(s) 928
TseI GCWGC 6 cut(s) 50, 392, 581, 662, 841, 863
TspDTI ATGAA 1 cut(s) 77
TspGWI ACGGA 3 cut(s) 184, 394, 608
TspRI CASTG 1 cut(s) 928
Van91I CCANNNNNTGG 1 cut(s) 692
VneI GTGCAC 1 cut(s) 607
XapI RAATTY 2 cut(s) 292, 886
XmiI GTMKAC 1 cut(s) 750
XspI CTAG 5 cut(s) 23, 57, 185, 719, 967
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.